Rhodamin B double-sulfur fluorescence probe for detecting aqueous mediums and intracellular mercury ions, preparation and application thereof

Abstract

The invention discloses a rhodamin B double-sulfur fluorescence probe for detecting aqueous mediums and intracellular mercury ions, preparation and application thereof. According to rhodamin B double-sulfur fluorescence probe, molecular closed loops in the rhodamin fluorescence probe are opened in the aqueous mediums by chelating and coordinating the mercury ions, so that fluorescence is generated; the fluorescence intensity is in a good linear relation with the initial concentration of the mercury ions; the color change can be seen by naked eyes when a probe solution is changed from colorless to pink; meanwhile, the fluorescence probe is high in specific selectivity on the mercury ions; in addition, the fluorescence probe is high in biocompatibility and cell permeability and low in cytotoxicity, can be used for detecting the intracellular mercury ions and is high in detection sensitivity of the mercury ions. The preparation of the rhodamin B double-sulfur fluorescence probe is characterized by taking the rhodamin B as the raw material, and enabling the rhodamin B to react with phosphorus oxychloride to prepare a rhodamin B acyl chloride intermediate; the acyl chloride intermediate is further condensed with a double-sulfur intermediate to obtain a mercury ion probe with high sensitivity and high selectivity.

Description

technical field

[0001] The invention belongs to the field of environmental detection and biological diagnosis, and particularly relates to a rhodamine B disulfide fluorescent probe for detecting aqueous medium and intracellular mercury ions, a preparation method and application thereof. Background technique

[0002] Mercury ion is one of the most threatening and toxic metal ions to human health. It has important toxic effects on the central nervous system, digestive system and kidneys of the human body, and also has certain effects on the respiratory system, skin, blood and eyes. Mercury and its pollutants are widely distributed in water, air and soil through volcanic activities, mining, solid waste incineration and other methods, and can be further enriched in the human body through the food chain, and the toxic effect on the human body is continuously enhanced , thereby posing a serious threat to human health. Based on this, the detection of low-concentration mercury ion...

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