This invention discloses a method and application for detecting
diazepam based on ratio Raman signals, belonging to the field of
food safety and chemical detection technology. The method involves mixing an Au@4-NTP@Ag@ZIF-8
composite material solution with the sample solution to be tested, then dropping the mixture onto a substrate. A Raman
spectrometer is used to acquire
spectral data at an
excitation wavelength of 532 nm, and the
intensity ratio I is calculated. 1590 / I 1107 Substitute
diazepam concentration to
intensity ratio I 1590 / I 1107 The concentration of
diazepam in the sample was calculated using a
standard curve. The detection method of this invention exhibits
high selectivity for diazepam and is unaffected by interference from macromolecules such as proteins, lipids, and glucose, as well as
small molecule chemicals in complex matrices. It enables trace, rapid, and accurate detection of diazepam residues in aquatic products, and has significant application prospects in practical scenarios such as port clearance and on-site law
enforcement.