11 results about "Protein staining" patented technology

The application discloses a laundry detergent with high decontamination and a preparation method thereof, and belongs to the technical field of liquid detergents. The laundry detergent comprises non-ionic surfactant, anionic surfactant, chelating agent, biological enzyme additive, plant essential oil, preservative and solvent. The preparation method comprises the following steps: adding non-ionic surfactant into the solvent, stirring and dissolving, then adding anionic surfactant, stirring and dissolving, then adding chelating agent, biological enzyme additive, plant essential oil and preservative, adjusting the pH value to 6-8, and obtaining a laundry detergent with high decontamination. The biological enzyme additive added in the laundry detergent can improve the removal capacity of the laundry detergent to protein stains and fat stains through enzymolysis reaction, and the essential oil is used as a natural antibacterial agent to reduce the addition of synthetic antibacterial agents and improve the environmental protection and degradability of raw materials. The laundry detergent prepared by the application has good decontamination effect and excellent antibacterial performance, and the product is efficient and stable.

The invention discloses a multi-modal biophysical characterization device, a multi-modal biophysical characterization system, a multi-modal biophysical characterization method and application thereof. The multi-modal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage and configurable physical characterization of single cells or multi-cell polymers can be realized, stimulation (such as mechanical stimulation, electrical stimulation and light stimulation) of different types and intensities can be performed on a to-be-detected sample, and the in-vivo microenvironment can be better simulated; and carrying out operations (such as marking, curing, ablation, cutting, extraction, sorting and the like) on samples at specific positions or regions, and carrying out comparative analysis in combination with other characterization methods (such as protein staining, histochemical staining, single cell sequencing and the like). The multi-mode biophysical characterization device is suitable for the fields of synthetic biology, diagnosis, drug discovery, tumor early screening, cell therapy, precision medical treatment and the like.

Provided is a mutant protease having improved cleaning performance against protein stains in a high-concentration detergent liquid. A mutant protease consisting of the amino acid sequence of SEQ ID NO: 1 or an amino acid sequence having an identity of at least 90% with SEQ ID NO: 1 and comprising at least one selected from the group consisting of the following amino acid residues (a) to (g): (a) Arg or Lys at a position corresponding to position 191 of SEQ ID NO: 1; (b) Arg or Lys at a position corresponding to position 17 of SEQ ID NO: 1; (c) Ala or Phe at a position corresponding to position 141 of SEQ ID NO: 1; (d) Arg or Lys at a position corresponding to position 243 of SEQ ID NO: 1; (e) Arg or Lys at a position corresponding to position 300 of SEQ ID NO: 1; (f) Arg or Lys at a position corresponding to position 302 of SEQ ID NO: 1; and (g) Arg or Lys at a position corresponding to position 311 of SEQ ID NO: 1.

The present invention discloses a method for three-dimensional spatial omics of tissues based on three-dimensional coordinate spatial positioning. The method includes performing XYZ three-dimensional spatial encoding on analytes such as DNA, RNA, proteins, chromatin, and sugars in tissue cells through multiple rounds of DNA ligation reactions, and then analyzing the corresponding spatial positions and biological information through sequencing technology, thereby realizing three-dimensional spatial omics analysis of tissues. The method for three-dimensional spatial omics of tissues based on three-dimensional coordinate spatial positioning of the present invention has the advantages of convenient operation, low technical threshold, low cost, etc., can obtain the three-dimensional spatial distribution of heterogeneous cells in intact tissues and the expression of corresponding biological information, and is widely applied to research in the directions of neuroscience, pathology, tissue development, etc.

This application provides a washing machine control method, apparatus, washing machine, and computer-readable storage medium. The method includes: acquiring turbidity data of the washing machine using a turbidity sensor; calculating a turbidity change rate and a turbidity acceleration based on the turbidity data; determining the stain type based on the turbidity change rate and turbidity acceleration; the stain type includes at least one of particulate stains, grease stains, and protein stains; and controlling the washing process of the washing machine according to the stain type. This method can improve the flexibility of washing machine control.

Provided are a nucleic acid detection primer combination or kit, a nucleic acid hybridization method, a method for detecting a target nucleic acid, and a method for joint detection of a nucleic acid and a protein. By using the primer combination and the detection method, the incubation time for fluorescence reading / detecting a hybridization signal for a nucleic acid (such as RNA) can be shortened, ensuring that after hybridization of a nucleic acid molecular signal, multiple rounds of protein staining can still be carried out on a sample, i.e. an RNA-protein joint detection method, which provides two types of evidence for staining a target site by means of detection results.

The present application relates to cell biology, and more specifically, to a multimodal biophysical characterization apparatus, a multimodal biophysical characterization system, a multimodal biophysical characterization method, and its use. The present application can achieve multimodal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage, and configurable physical characterization of single cells or multicellular aggregates, and can subject the sample to be tested to stimuli of different types and intensities (such as mechanical stimulation, electrical stimulation, light stimulation, etc.) to better simulate the in vivo microenvironment, as well as perform operations on samples at specific positions or regions (such as marking, fixing, ablating, cutting, extracting, sorting, etc.), and combine with other characterization methods (such as protein staining, histochemical staining, single-cell sequencing, etc.) for comparative analysis. The multimodal biophysical characterization apparatus provided by the present application is suitable for fields such as synthetic biology, diagnostics, drug discovery, early tumor screening, cell therapy, and precision medicine.

The present invention relates to a washing process for improving protein stain removal on textiles and to the use of chelating agents for improving protein stain removal.

To shift an object to be washed from preliminary washing to main washing at a higher temperature without damaging detergency of proteinic stain, regarding a business-use washing device which performs steps of preliminary washing, main washing, rinsing, finishing and dewatering of an object to be washed sequentially.SOLUTION: A continuous type washing machine includes temperature raising means which increases the temperature of an object to be washed and preliminary washing water in a preliminary washing tub during a preliminary washing step of an inputted object to be washed and whose heat source is recovery heat. The temperature raising means starts an operation after the time set after the object to be washed is inputted in the preliminary washing tub or when the object to be washed is transferred to the next stage preliminary washing tub, and finishes the operation before the object to be washed is transferred to a main washing tub.SELECTED DRAWING: Figure 1

The invention discloses a laundry detergent with high detergency and a preparation method thereof, and belongs to the technical field of liquid detergents, the laundry detergent comprises a nonionic surfactant, an anionic surfactant, a chelating agent, a biological enzyme additive, plant essential oil, a preservative and a solvent, the preparation method comprises the following steps: adding the nonionic surfactant into the solvent, stirring and dissolving, then adding the anionic surfactant, stirring and dissolving, then adding the chelating agent, the biological enzyme additive, the plant essential oil and the preservative, and adjusting the pH value to 6-8 to obtain the laundry detergent with high detergency. The bio-enzyme additive added in the laundry detergent can improve the ability of the laundry detergent to remove protein stains and fat stains through enzymolysis reaction, and the essential oil is supplemented as a natural antibacterial agent, so that the addition of synthetic antibacterial agents is reduced, and the environment-friendly degradability of the raw materials is improved. The prepared laundry detergent has a good decontamination effect and excellent antibacterial performance, and the product is efficient and stable.

This application relates to the field of cell biology, and more specifically to a multimodal biophysical characterization apparatus, a multimodal biophysical characterization system, a multimodal biophysical characterization method, and its applications. This application enables multimodal, high-resolution, high-throughput, high-sensitivity, low-cost, low-damage, and configurable physical characterization of single cells or multicellular aggregates, and allows for the application of different types and intensities of stimuli (e.g., mechanical, electrical, or optical stimulation) to the sample under measurement to better mimic the in vivo microenvironment, and allows for manipulation (e.g., labeling, fixation, ablation, cutting, extraction, or sorting) of samples at specific locations or regions, and can be combined with other characterization methods (e.g., protein staining, histochemical staining, or single-cell sequencing) for comparative analysis. The multimodal biophysical characterization apparatus according to this application is suitable for fields such as synthetic biology, diagnostics, drug discovery, early tumor screening, cell therapy, and precision medicine.