Efficient biological enzyme detergent as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of biochemical environmental protection, and particularly discloses an efficient biological enzyme detergent which comprises a lipolytic enzyme SEQ NO: 1, a high-temperature-resistant amylolytic enzyme SEQ NO: 2, an alkali-resistant proteolytic enzyme SEQ NO: 3, a novel slow-release organic compound Compunder X and cocamidopropyl betaine and other auxiliaries. The biological enzyme has synergistic interaction aiming at different stains, the Compound X contains long-chain alkyl, ester group and hydroxyl, the long-chain alkyl is oleophylic, the ester group slowly releases and cleans, and the hydroxyl assists in dissolving and stabilizes the enzyme. The product has the advantages of excellent decontamination performance, obviously improved cleaning degree on oil stain, starch and protein stains compared with the traditional detergent, environmental protection, safety, good formula synergism, no damage to tableware materials, and wide prospect in the field of kitchen cleaning.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine and chemistry, and specifically relates to a high-efficiency biological enzyme detergent and a preparation method and application thereof. Background Art

[0002] In daily life, kitchen grease is a common and difficult cleaning problem. Traditional dishwashing liquids mainly rely on chemical synthetic surfactants to achieve the decontamination function, such as sodium alkylbenzene sulfonate. Although these chemical surfactants can emulsify and disperse grease to a certain extent, there are many disadvantages in long-term use. First, a large number of chemical synthetic surfactants are difficult to be quickly degraded by the natural environment after discharge, and are easily accumulated in water bodies and soil, causing continuous pollution to the ecological environment and threatening the survival and ecological balance of aquatic organisms. Secondly, some chemical substances may remain on the surface of tableware. If they are not thoroughly cleaned, they may cause potential harm to human health as people ingest them through their diet, such as causing gastrointestinal discomfort and allergic reactions. As a natural biocatalyst, bioenzyme is produced by microbial fermentation and has significant advantages such as high efficiency, specificity, and environmental protection. It can catalyze specific chemical reactions under mild conditions and decompose large molecular organic matter into small molecular substances, making it easier to be cleaned and removed. However, existing bio-enzyme dishwashing liquids still have deficiencies in enzyme type selection, activity maintenance, and overall formula optimization, resulting in unstable decontamination effects and limited removal of stubborn oil stains, making it difficult to meet consumers' demand for efficient, safe, and environmentally friendly kitchen cleaning products. Therefore, the development of a new type of efficient bio-enzyme dishwashing liquid is of great practical significance. Summary of the invention

[0003] The present invention provides a high-efficiency biological enzyme detergent, comprising the following components in weight percentage: lipolytic enzyme SEQ NO: 1 Ser-Leu-Ile-His-Asp-Gly-Lys-Thr: 4 parts to 10 parts;

[0004] High temperature resistant starch decomposing enzyme SEQ NO: 2Tyr-Asn-Glu-Val-Ala-Met-Pro-Phe: 3-8 parts; alkaline resistant protein decomposing enzyme SEQ NO: 3Arg-Cys-Gln-Thr-Leu-Ile-His-Ser: 3-7 parts; new sustained-release organic compound Compound X: 3-hydroxy-2-(dodecyloxycarbonyl)propyl-2-hydroxypropionate: 5-10 parts; surfactant: 10-20 parts of cocamidopropyl betaine, 8-15 parts of alkyl glycoside; cosolvent: 5-10 parts of ethanol, 6-12 parts of glycerol; chelating agent: 1-3 parts of sodium citrate; preservative: 0.1-0.5 parts of potassium sorbate; flavor: 0.05-0.2 parts of lemon flavor.

[0005] Furthermore, the lipolytic enzyme SEQ NO: 1 is dissolved in a phosphate buffer solution with a pH value of 7-9, the high temperature resistant starch decomposing enzyme SEQ NO: 2 is dissolved in a Tris-HCl buffer solution with a pH value of 6-8, and the alkaline resistant protein decomposing enzyme SEQ NO: 3 is dissolved in a glycine-sodium hydroxide buffer solution with a pH value of 8-10, and the enzyme concentration reaches 15 parts to 25 parts w / v before being added to the detergent system.

[0006] Furthermore, the preparation method of the novel sustained-release organic compound Compound X is as follows: in a four-necked flask equipped with a stirrer, a thermometer, a reflux condenser and a dropping funnel, a certain amount of dodecanol and a small amount of concentrated sulfuric acid are added, stirred and heated to 60-70°C; acrylic acid is slowly added dropwise, the dropping speed is controlled to maintain the reaction temperature at 70-80°C, and after the addition is completed, the reaction is continued for 2-3 hours to obtain dodecyl acrylate.

[0007] Furthermore, the preparation method of the novel sustained-release organic compound Compound X also includes: washing with a saturated sodium carbonate solution until neutral, drying with anhydrous magnesium sulfate, and distilling under reduced pressure to obtain purified dodecyl acrylate; adding 3-hydroxypropionic acid and p-toluenesulfonic acid to an ethanol solution, stirring and dissolving, and heating to 70-80° C. Slowly dripping the prepared dodecyl acrylate, and after the dripping is completed, reflux reaction for 4-6 hours.

[0008] Furthermore, the preparation method of the novel sustained-release organic compound Compound X also includes: monitoring the progress of the reaction by thin-layer chromatography during the reaction; after the reaction is completed, cooling to room temperature, adjusting the pH value to neutral with a saturated sodium carbonate solution, and filtering to remove the generated salt. The filtrate is decompressed and distilled to remove ethanol to obtain a crude product; the crude product is purified by column chromatography, using petroleum ether / ethyl acetate in a volume ratio of 8:2-6:4 as an eluent, and the target product fraction is collected to obtain 3-hydroxy-2-(dodecyloxycarbonyl)propyl-2-hydroxypropionate.

[0009] Further, the preparation method thereof comprises the following steps:

[0010] Prepare solutions of lipolytic enzyme SEQ NO: 1, thermostable starch decomposing enzyme SEQ NO: 2 and alkaline-resistant protein decomposing enzyme SEQ NO: 3 respectively;

[0011] Dissolve Compound X in an appropriate amount of ethanol solution to prepare a solution with a concentration of 5-10 parts (w / v); add cocamidopropyl betaine (CAPB) and alkyl polyglycoside (APG) into deionized water, and stir to dissolve to form a surfactant solution;

[0012] Add ethanol and glycerol dissolved in Compound X, sodium citrate and potassium sorbate to the surfactant solution in turn, and stir until completely dissolved and mixed evenly; slowly add the bioenzyme solution to the above mixed solution and stir evenly; add lemon essence, stir evenly, adjust the total volume with deionized water, fine-tune the pH value to 7-9, filter to remove impurities, obtain the finished product and package it.

[0013] On the other hand, the present invention also provides an application of the high-efficiency biological enzyme detergent in kitchen cleaning, which is used to remove oil stains, starch stains, protein stains and other common stains on tableware, kitchen utensils and kitchen countertops, protect the material of the cleaning object, and is suitable for materials such as ceramics, glass, stainless steel, and plastic. Beneficial effects of the invention:

[0014] The biological enzyme detergent of the present invention contains fat decomposing enzymes, high temperature resistant starch decomposing enzymes and alkaline resistant protein decomposing enzymes for different types of stains, and there is a synergistic effect between the three biological enzymes, so it can efficiently remove a variety of complex stains in the kitchen. Whether it is grease stains (edible oil, animal fats, etc.), starch stains (rice, noodles, batter, etc.) or protein stains (meat, eggs, milk residues, etc.), they can all be thoroughly cleaned. Compared with traditional detergents, under the same washing conditions, the cleaning degree of complex stains can be increased by 40%-60%, which can make tableware and kitchen supplies cleaner and tidier, effectively solve the problem of cleaning kitchen oil stains, and meet consumers' demand for high-quality kitchen cleaning products.

[0015] As a natural biocatalyst, bioenzyme has good biodegradability and can be quickly decomposed into harmless substances in the natural environment. It will not pollute water and soil. Compared with the chemical synthetic ingredients in traditional dishwashing liquids, it is more friendly to the ecological environment. At the same time, bioenzyme itself is non-toxic to the human body, and other ingredients in dishwashing liquids, such as surfactants from natural plants and food-grade preservatives, are also highly safe. Even if there is a small amount of residue during the dishwashing process, it will not harm human health. In addition, it is gentle and non-irritating to the skin, and it is not easy to cause skin allergies, dryness and other problems even with frequent contact. It is particularly suitable for home use and places such as the catering industry that have high requirements for the safety of cleaning products.

[0016] The surfactants, cosolvents, chelating agents, preservatives and flavors in the detergent cooperate with the biological enzymes to play a synergistic role. The surfactant compounding improves the decontamination effect and stability, the cosolvents ensure the stability and performance of the system, the chelating agent improves the hard water adaptability, the preservatives ensure the product quality and safety, and the flavors give a pleasant fragrance. The addition of Compound X further enhances the decontamination effect, and its slow-release characteristics make the cleaning effect more durable. There is also a synergistic effect with the biological enzymes, which improves the overall cleaning performance by stabilizing the enzyme structure and continuously providing emulsification and dispersion capabilities. The various components complement each other, so that the detergent not only performs well in decontamination, but also has good stability, adaptability and use experience, has strong competitiveness in the cleaning products market, and provides consumers with a kitchen cleaning product with excellent comprehensive performance. During the cleaning process, the biological enzyme detergent of the present invention will not damage the material of the tableware due to the specific action of the biological enzyme and the mild formula design. Unlike the strongly alkaline or strongly oxidizing ingredients in some traditional dishwashing liquids, it can be safely used to clean a variety of common tableware and kitchenware materials such as ceramic, glass, stainless steel, and plastic. It will not cause problems such as fading, deformation, and corrosion of tableware, thereby extending the service life of tableware and kitchenware and, in the long run, reducing consumers' usage costs. DETAILED DESCRIPTION

[0017] Example 1

[0018] The detergent of the present invention comprises the following key biological enzymes:

[0019] Lipolytic enzyme: The present invention adopts a novel lipolytic enzyme SEQ NO: 1: Ser-Leu-Ile-His-Asp-Gly-Lys-Thr (SLIHDGKT). Its action principle is based on the specific interaction between the amino acid residues in the active center and the oil molecule. Among them, the His and Asp residues constitute part of the catalytic triad, which promotes the hydrolysis of the ester bond in the oil molecule through the acid-base catalytic mechanism. The Ser residue attacks the carbonyl carbon of the ester bond as a nucleophilic reagent, initiating the hydrolysis reaction. Leu, Ile and Gly residues play a key role in the structural construction of the enzyme molecule and the formation of the substrate binding pocket. Their hydrophobic side chains interact with the hydrophobic part of the oil molecule, enhancing the affinity of the enzyme for the oil, so that the enzyme can be more accurately positioned and act on the oil stains. Lys and Thr residues help maintain the overall structural stability of the enzyme and the suitable environment of the active center, ensuring that the lipolytic enzyme can effectively decompose oil stains, such as edible oil, animal fats and other common kitchen oil stains under different conditions.

[0020] Amylolytic enzyme: A thermostable amylolytic enzyme SEQ NO: 2: Tyr-Asn-Glu-Val-Ala-Met-Pro-Phe (YNEVAMPF) was selected. The enzyme can specifically recognize and catalyze the cleavage of glycosidic bonds in starch molecules. The Glu and Asp residues in its active center participate in the acid-base catalysis process, promoting the hydrolysis reaction of glycosidic bonds. The aromatic ring structure and hydrophobic side chains of Tyr, Phe and Val residues interact with the non-polar region of starch molecules, enhancing the binding ability of the enzyme to starch stains. Asn, Ala and Met residues play an important role in constructing substrate binding sites and maintaining the stability of the enzyme structure, while the Pro residue can increase the rigidity and stability of the enzyme molecule, so that the thermostable amylolytic enzyme can still maintain good activity at higher temperatures, effectively decompose starch-containing stains attached to tableware, such as residual stains such as rice, noodles, and batter, and can function stably even under hot water washing conditions.

[0021] Proteolytic enzyme: An alkaline-resistant proteolytic enzyme SEQ NO: 3: Arg-Cys-Gln-Thr-Leu-Ile-His-Ser (RCQTLIHS) is used. The active center of this enzyme can specifically act on the peptide bonds in protein molecules. The His residue acts as an acid-base catalyst in the active center, synergistically with other amino acid residues to break the peptide bonds. The Arg residue is positively charged and can form electrostatic interactions with the acidic amino acid residues in the protein molecule, enhancing the binding force between the enzyme and protein stains. The Cys residue can form a disulfide bond, which helps to maintain the structural stability of the enzyme, especially in an alkaline environment. Gln, Thr, Leu, Ile and Ser residues together constitute the substrate binding region of the enzyme, enabling it to efficiently identify and decompose protein stains, such as protein components such as meat, eggs, and milk in food residues. It shows excellent activity in an alkaline washing environment and effectively removes protein stains on tableware.

[0022] In dishwashing liquid, these three biological enzymes cooperate with each other to produce a synergistic cleaning effect. In kitchen oil stains, there are often grease, starch and protein stains at the same time. For example, for stains containing a mixture of grease and protein (such as the oil stains on the bottom of the pot after frying meat), the lipase first decomposes the grease part, hydrolyzes the macromolecular grease into small molecules such as fatty acids and glycerol, so that the protein stains originally wrapped in grease are exposed. At this time, the alkaline-resistant protease can act more effectively on the protein stains and decompose them into small molecular polypeptides and amino acids. At the same time, the decomposition of protein stains also helps the lipase to further penetrate into the oil stains and accelerate the decomposition of grease, because the degradation of protein changes the overall structure and physical properties of the oil stains. For stains containing a mixture of starch and grease (such as tableware that has been used to hold noodles and has grease), the high-temperature resistant starch enzyme decomposes the starch stains, breaks the starch molecular chain, reduces the stickiness and adhesion of the stains, and creates conditions for the lipase to better contact and decompose the grease stains, while the decomposition of grease is conducive to the starch enzyme to completely remove the residual starch stains. When the three enzymes work together, the decomposition products of complex stains can be quickly emulsified and dispersed in the detergent, preventing them from redepositing on the surface of tableware, thereby greatly improving the cleaning ability of various complex stains in the kitchen. Compared with single enzyme detergents or traditional detergents, there are significant improvements in cleaning degree and cleaning efficiency.

[0023] The present invention also includes an unreported slow-release organic compound, named Compound X. The chemical structure of Compound X contains a long-chain alkyl group, an ester group and multiple hydroxyl groups. Design principle: The long-chain alkyl group of Compound X has strong hydrophobicity, which enables it to interact with the hydrophobic components in the oil stains and enhance the adsorption capacity of the oil stains. The ester group can undergo a slow hydrolysis reaction in water, and gradually releases small molecules with surface activity during the hydrolysis process. These small molecules can further emulsify and disperse the oil stains, thereby achieving a slow-release cleaning effect. Multiple hydroxyl groups increase the water solubility of the compound and its hydrogen bonding ability with water molecules, allowing it to be better dispersed in water, and the hydroxyl groups can also form hydrogen bonding interactions with certain amino acid residues in the biological enzyme molecules, stabilizing the active structure of the enzyme to a certain extent, and promoting the decomposition of stains by the biological enzyme.

[0024] The preparation method of the novel sustained-release organic compound Compound X is as follows: in a four-necked flask equipped with an agitator, a thermometer, a reflux condenser and a dropping funnel, a certain amount of dodecanol and a small amount of concentrated sulfuric acid are added, stirred and heated to 60-70°C; acrylic acid is slowly dripped, the dripping speed is controlled, the reaction temperature is maintained at 70-80°C, and the reaction is continued for 2-3 hours after the dripping is completed to obtain dodecyl acrylate. Wash with a saturated sodium carbonate solution to neutrality, then dry with anhydrous magnesium sulfate, and distill under reduced pressure to obtain purified dodecyl acrylate; 3-hydroxypropionic acid and p-toluenesulfonic acid are added to the ethanol solution, stirred and dissolved, and heated to 70-80°C. The prepared dodecyl acrylate is slowly dripped, and refluxed for 4-6 hours after the dripping is completed. The reaction progress is monitored by thin layer chromatography during the reaction; after the reaction is completed, it is cooled to room temperature, the pH value is adjusted to neutral with a saturated sodium carbonate solution, and the generated salt is filtered out. The filtrate was distilled under reduced pressure to remove ethanol to obtain a crude product; the crude product was purified by column chromatography using petroleum ether / ethyl acetate in a volume ratio of 8:2-6:4 as an eluent, and the target product fractions were collected to obtain 3-hydroxy-2-(dodecyloxycarbonyl)propyl-2-hydroxypropionate.

[0025] The bio-enzyme detergent of the present invention contains the following ingredients in addition to the above-mentioned bio-enzyme and Compound X:

[0026] Surfactants from natural plant sources, such as cocamidopropyl betaine (CAPB) and alkyl polyglycoside (APG) are used in combination. CAPB is a zwitterionic surfactant with good detergency, foaming properties and biocompatibility. The amide group and quaternary ammonium group in its molecular structure enable it to maintain stable surface activity under different pH conditions, effectively remove stains on the surface of tableware, and is mild and non-irritating to the skin. APG is a new type of non-ionic surfactant synthesized from renewable resources, natural fatty alcohol and glucose. It has excellent emulsification, dispersibility and wetting properties, is easily biodegradable, and is environmentally friendly. Its glycoside structure enables it to form hydrogen bonds with water, enhancing its solubility in water. When combined with CAPB, it can play a synergistic role and improve the detergency effect of detergents, especially the emulsification and dispersibility of oil stains, and maintain good stability and cleaning performance under different water quality conditions.

[0027] Cosolvent: Add ethanol and glycerin. Ethanol can increase the solubility of other ingredients in water, making the system of detergent more uniform and stable. At the same time, it has a certain bactericidal and disinfecting effect, which can inhibit the growth of microorganisms during the storage and use of detergent. Glycerin has good moisturizing properties, which can prevent the detergent from drying and hardening due to water evaporation during use, maintaining its good fluidity and performance. Glycerin also has a moisturizing effect on the skin, which can reduce the dryness and irritation of the skin caused by detergent, so that the skin of the hands can remain relatively soft and comfortable after frequent contact with detergent.

[0028] Chelating agent: Use sodium citrate. Kitchen water may contain metal ions such as calcium and magnesium. These metal ions will combine with surfactants to form precipitation, reduce the activity of surfactants, and affect the cleaning effect of detergents. Sodium citrate can form stable chelates with metal ions, chelate metal ions, and prevent them from reacting with surfactants, thereby ensuring that detergents can also play a normal cleaning role in hard water or water containing metal ions, and improve the adaptability and cleaning performance of detergents.

[0029] Preservative: Potassium sorbate is used. It can inhibit the growth and reproduction of microorganisms during the production, storage and use of dishwashing liquid, ensure the quality and safety of the product, and extend the shelf life of the product. Potassium sorbate is a commonly used food-grade preservative with low toxicity and irritation. At the specified concentration, it has little impact on human health and the environment, and meets the requirements of environmental protection and safety.

[0030] Fragrance: Add lemon essence. Lemon essence can give the detergent a fresh lemon scent, making the dishes emit a pleasant aroma after washing, effectively removing kitchen odors, bringing users a pleasant experience, and also making the kitchen environment more refreshing and pleasant.

[0031] First, three biological enzyme solutions are prepared respectively. The lipolytic enzyme SEQ NO: 1, the thermostable starch decomposing enzyme SEQ NO: 2 and the alkaline-resistant proteolytic enzyme SEQ NO: 3 are dissolved in an appropriate amount of buffer solution, and the pH value of the buffer solution is adjusted according to the optimal pH value of each enzyme to ensure the activity of the enzyme. For example, the lipolytic enzyme is dissolved in a phosphate buffer solution with a pH value of 7-9, the thermostable starch decomposing enzyme is dissolved in a Tris-HCl buffer solution with a pH value of 6-8, and the alkaline-resistant proteolytic enzyme is dissolved in a glycine-sodium hydroxide buffer solution with a pH value of 8-10, so that the concentration of the enzyme reaches 15%-25% (w / v). Compound X is dissolved in an appropriate amount of ethanol solution to prepare a solution with a concentration of 5%-10% (w / v). Cocoamidopropyl betaine (CAPB) and alkyl glucoside (APG) are added to deionized water, and stirred in a stirrer at a speed of 400-600 rpm to fully dissolve them to form a surfactant solution. Add the cosolvent ethanol (Compound X has been dissolved) and glycerol, the chelating agent sodium citrate, and the preservative potassium sorbate to the surfactant solution in turn, and continue stirring until all the ingredients are completely dissolved and mixed evenly. Slowly add the pre-prepared bio-enzyme solution to the above mixed solution, stirring while adding, and control the stirring speed at 300-500 rpm to ensure that the bio-enzyme is evenly dispersed in the detergent system to avoid enzyme inactivation. Finally, add the lemon flavor, stir evenly, adjust the total volume of the detergent with deionized water so that the concentration of each component reaches the specified range, and use citric acid or sodium hydroxide solution to fine-tune the pH value to 7-9 to ensure the stability of the detergent and the activity of the bio-enzyme. Then filter to remove possible impurities and insoluble particles to obtain a high-efficiency bio-enzyme detergent product, and package it.

[0032] The following three detergent samples were prepared:

[0033] Sample A: contains only lipolytic enzyme SEQ NO: 1 (4%), thermostable starch-degrading enzyme SEQ NO: 2 (3%), alkaline-resistant protein-degrading enzyme SEQ NO: 3 (3%), cocamidopropyl betaine (CAPB) (10%), alkyl glycoside (APG) (8%), ethanol (5%), glycerol (6%), sodium citrate (1%), potassium sorbate (0.1%), lemon essence (0.05%), and the rest is deionized water, and is prepared according to the above preparation method.

[0034] Sample B: Based on sample A, Compound X (5%) was added and the sample was prepared according to the above preparation method.

[0035] Sample C: a traditional brand of dishwashing liquid on the market.

[0036] Tableware of the same material (ceramic) and similar degree of contamination was selected and cleaned with the three detergents mentioned above. The stains on the tableware were mixed with equal amounts of cooking oil, rice and egg liquid. The cleaning conditions were: 10 ml of detergent was added to 1 liter of 40°C water, the tableware was soaked for 8 minutes, then wiped with the same sponge at the same intensity for 2 minutes, and finally rinsed with clean water.

[0037] The amount of stain residue on the surface of tableware after washing was tested with a professional stain residue detection instrument. The results showed that sample A had relatively more stain residue after washing, with a cleaning degree of 65%. Sample C had a cleaning degree of 50%. The amount of stain residue in sample B after washing was significantly reduced, with a cleaning degree of 85%. This shows that the addition of CompoundX significantly improves the decontamination effect of detergent, and produces a synergistic effect with biological enzymes and other ingredients, greatly improving the detergent's ability to clean complex stains.

Claims

1. A highly efficient biological enzyme detergent, characterized in that: The composition comprises the following ingredients in weight percentage: lipolytic enzyme SEQ NO: 1Ser-Leu-Ile-His-Asp-Gly-Lys-Thr: 4 parts to 10 parts; High temperature resistant starch decomposing enzyme SEQ NO: 2Tyr-Asn-Glu-Val-Ala-Met-Pro-Phe: 3-8 parts; Alkaline resistant protein decomposing enzyme SEQ NO: 3Arg-Cys-Gln-Thr-Leu-Ile-His-Ser: 3-7 parts; New sustained-release organic compound Compound X: 3-hydroxy-2-(dodecyloxycarbonyl)propyl-2-hydroxypropionate: 5-10 parts; Surfactant: 10-20 parts of cocamidopropyl betaine, 8-15 parts of alkyl glycoside; Cosolvent: 5-10 parts of ethanol, 6-12 parts of glycerol; Chelating agent: 1-3 parts of sodium citrate; Preservative: potassium sorbate 0.1-0.5 parts; Flavor: lemon flavor 0.05 parts - 0.2 parts.

2. The high-efficiency biological enzyme detergent according to claim 1, characterized in that: The lipolytic enzyme SEQ NO: 1 is dissolved in a phosphate buffer solution with a pH value of 7-9, the high temperature resistant starch decomposing enzyme SEQ NO: 2 is dissolved in a Tris-HCl buffer solution with a pH value of 6-8, and the alkaline resistant protein decomposing enzyme SEQ NO: 3 is dissolved in a glycine-sodium hydroxide buffer solution with a pH value of 8-10, and the enzyme concentration reaches 15 parts to 25 parts w / v before being added to the detergent system.

3. The high-efficiency biological enzyme detergent according to claim 1, characterized in that: The preparation method of the novel sustained-release organic compound Compound X is as follows: a certain amount of dodecanol and a small amount of concentrated sulfuric acid are added to a four-necked flask equipped with a stirrer, a thermometer, a reflux condenser and a dropping funnel, and the mixture is stirred and heated to 60-70° C.; acrylic acid is slowly added dropwise, and the dropping speed is controlled to maintain the reaction temperature at 70-80° C. After the addition is completed, the reaction is continued for 2-3 hours to obtain dodecyl acrylate.

4. The high-efficiency biological enzyme detergent according to claim 1, characterized in that: The preparation method of the novel sustained-release organic compound Compound X also includes: washing with a saturated sodium carbonate solution until neutral, drying with anhydrous magnesium sulfate, and distilling under reduced pressure to obtain purified dodecyl acrylate; adding 3-hydroxypropionic acid and p-toluenesulfonic acid to an ethanol solution, stirring and dissolving, and heating to 70-80° C. Slowly drop the prepared dodecyl acrylate, and after the dropwise addition is completed, reflux for reaction for 4-6 hours.

5. The high-efficiency biological enzyme detergent according to claim 1, characterized in that: The preparation method of the novel sustained-release organic compound Compound X also includes: monitoring the progress of the reaction by thin-layer chromatography during the reaction; after the reaction is completed, cooling to room temperature, adjusting the pH value to neutral with a saturated sodium carbonate solution, and filtering to remove the generated salt. The filtrate is decompressed and distilled to remove ethanol to obtain a crude product; the crude product is purified by column chromatography, using petroleum ether / ethyl acetate in a volume ratio of 8:2-6:4 as an eluent, and the target product fraction is collected to obtain 3-hydroxy-2-(dodecyloxycarbonyl)propyl-2-hydroxypropionate.

6. The high-efficiency biological enzyme detergent according to claim 1, wherein the preparation method comprises the following steps: Prepare solutions of lipolytic enzyme SEQ NO: 1, thermostable starch decomposing enzyme SEQ NO: 2 and alkaline-resistant protein decomposing enzyme SEQ NO: 3 respectively; Dissolve Compound X in an appropriate amount of ethanol solution to prepare a solution with a concentration of 5-10 parts (w / v); add cocamidopropyl betaine and alkyl glucoside into deionized water, and stir to dissolve to form a surfactant solution; Add ethanol and glycerol dissolved in Compound X, sodium citrate and potassium sorbate to the surfactant solution in turn, and stir until completely dissolved and mixed evenly; slowly add the bioenzyme solution to the above mixed solution and stir evenly; add lemon essence, stir evenly, adjust the total volume with deionized water, fine-tune the pH value to 7-9, filter to remove impurities, obtain the finished product and package it.

7. An application of the high-efficiency bio-enzyme detergent as described in any one of claims 1 to 6 in kitchen cleaning, for removing oil stains, starch stains, protein stains and other common stains from tableware, kitchen utensils and kitchen countertops, protecting the material of the cleaning object, and is suitable for materials such as ceramics, glass, stainless steel, and plastic.