39 results about "Biological toxin" patented technology

The invention discloses a composite functional bacterial agent for preventing and controlling wheat scab and vomitoxin and application of the composite functional bacterial agent. The composite functional bacterial agent is applied to wheat scab and deoxynivalenol pollution caused by fusarium graminearum. The composite functional bacterial agent comprises a biocontrol strain bacillus subtilis DC-1 (Bacillus subtilis DC-1) and a degradation strain nocardia sp. MOZ-1 (Nocardia sp. MOZ-1), and the composite functional bacterial agent is prepared from the biocontrol strain bacillus subtilis DC-1, the degradation strain nocardia sp. MOZ-1 and the degradation strain nocardia sp. MOZ-1. The complex microbial inoculant can significantly inhibit infection of fusarium graminearum and reduce accumulation of DON in wheat, is low in production cost, reduces use of chemical pesticides and pollution of biotoxins in agricultural products, and can be effectively applied to the field of agricultural biological control.

The invention relates to the technical field of biotoxin detection, and discloses a multidimensional data analysis-based fungaltoxin six-in-one detection card intelligent interpretation system and method.The method comprises the following steps: collecting first toxin detection data of six fungaltoxins, including a fluorescence response time sequence, a colloidal gold response time sequence and an environment variable time sequence; performing feature extraction on the second toxin detection data to obtain second toxin detection data; then establishing a historical detection database, constructing and training a mycotoxin classification model, and obtaining a preliminary recognition result of the six mycotoxins according to the second toxin detection data and the classification model; and finally, carrying out weighted fusion on the preliminary recognition result in combination with an environment variable time sequence to obtain an accurate recognition result. According to the invention, intelligent interpretation of mycotoxin detection is realized, and the detection accuracy and efficiency are greatly improved.

The invention relates to the technical field of vaccines, and discloses a preparation method and application of an anti-tumor vaccine based on an autoantigen and a synthetic antigen. The anti-tumor vaccine comprises a tumor autoantigen and a synthetic antigen, wherein the tumor autoantigen is extracted from a tumor cell membrane, a tumor cell lysate, a tumor cell secretion, an exosome antigen or an antigen released by a tumor microenvironment; the synthetic antigen is selected from at least one of a tumor associated antigen, a tumor specific antigen, a personalized neoantigen, a pathogen antigen, a biotoxin, a biomolecule antigen and coding nucleic acid of a peptide type antigen in the antigens; the coding nucleic acid is DNA or mRNA. The vaccine molecule composed of the tumor autoantigen and the synthetic antigen is easy to prepare, has a strong anti-tumor immune effect and good biological safety, solves the problems that the traditional tumor autoantigen has no specific precise target spot and the synthetic antigen is single in immunogen, and has a wide application prospect.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

The invention relates to the technical field of intelligent medical first aid and data processing, in particular to an intelligent first aid information studying and judging platform for war wounds and biological injuries. The system comprises a data acquisition module, a feature reconstruction module, an entropy research and judgment module and a decision generation module. The system executes causal inference decoupling based on the composite physiological data and the environmental parameters; the method is characterized in that dominant trauma and recessive biological injury characteristics are separated, study and judgment confidence and user execution entropy are calculated, and an emergency treatment strategy is determined according to the game relationship of the two; according to the method, the non-linear physiological masking effect caused by biological factors is effectively stripped, the problem of misjudgment caused by the fact that biological toxins cover wound signs is solved, and acute capture and early warning of recessive biological injuries are achieved.

The invention discloses a marine organism toxin detection system and a marine organism toxin detection method, and belongs to the technical field of marine organism toxin detection. The system comprises an acquisition unit and a processing unit, an automatic sample changing device in the collecting unit is used for transferring the samples collected by the sampling device to the processing unit; a primary screening device in the processing unit comprises a sampler and a voltage-gated ion channel, the sampler inputs samples conveyed by the automatic sample changing device into the voltage-gated ion channel, and the samples are preliminarily screened through the voltage-gated ion channel; through preliminary screening, different components in a sample enter corresponding electrochemical sensors respectively, specific binding of an antigen and an antibody is realized, an electric signal is generated, and detection of marine biological toxins is realized. According to the invention, the design of secondary detection is applied, the problem of cross reaction is solved, and the method has extremely high sensitivity and selectivity, does not need any harmful reagent, is high in safety, does not generate secondary pollutants, and is environment-friendly.

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.

The invention belongs to the technical field of medicines, and particularly relates to a medicine for treating various biotoxins. The traditional Chinese medicine composition is prepared from the following components in parts by mass: 20 to 40 parts of radix sophorae flavescentis, 20 to 40 parts of fructus kochiae, 20 to 40 parts of herba taraxaci, 20 to 40 parts of liquorice root, 10 to 15 parts of fructus forsythiae, 10 to 15 parts of flos chrysanthemi indici, 10 to 15 parts of gynura bicolor, 10 to 15 parts of fructus cnidii and 10 to 15 parts of cortex dictamni. , 15-20 parts by mass of moleplant seeds, 22-26 parts by mass of bauhinia championii and 28-36 parts by mass of sculellaria barbata. The preparation method of the medicine comprises the following steps: step 1, drying the medicine; step 2, weighing the medicines in corresponding parts by mass; step 3, grinding the medicines; and step 4, finally mixing the medicines with wine or water. The traditional Chinese medicine can be used for quickly treating bite wounds of poisonous bees, scorpions, green snakes and green stauntonia moth, and is quick in treatment effect and good in effect.

A method of removing at least one of algae, bacteria, microorganisms, toxins, contaminants, excess nutrients and toxic substances from water, involving placing into or on at least one of the soil and the water a structure comprising an open-cell foam material together with a second material that is configured to adsorb or digest the at least one of algae, bacteria, microorganisms, toxins, contaminants, excess nutrients, and toxic substances, leaving the structure in place for sufficient time to adsorb or digest the at least one of algae, bacteria, microorganisms, toxins, contaminants, excess nutrients, and toxic substances, and then removing the structure from the at least one of the soil and the water.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The invention relates to the technical field of biological adsorption materials, in particular to modified peptidoglycan with an efficient adsorption effect and a preparation method and application of the modified peptidoglycan. The preparation method comprises the steps of grafting and modifying carboxyl / hydroxyl of peptidoglycan, so that multi-site and multi-mode functional modification on the surface of the peptidoglycan is realized. The physicochemical properties of functional groups on the surface of peptidoglycan are changed through modification treatment, the density and electronegativity of carboxyl and hydroxyl functional groups on the surface of peptidoglycan are improved, the hydrogen-bond interaction and electrostatic adsorption capacity between the peptidoglycan and biotoxin are enhanced, and the adsorption efficiency is improved. In addition, the peptidoglycan is subjected to carboxyl and hydroxyl modification at the same time, and the tetrodotoxin adsorption effect of the peptidoglycan can be further improved. The technology provides a new strategy for functionalization of natural polymer materials, has good theoretical popularization value and application prospects, and is suitable for the fields of food safety, removal and purification of biotoxins and the like.

The invention provides a variable-temperature infrared spectrum calculation method for protein biotoxins, which comprises the following steps of: putting a macromolecular bioprotein structure into a box with a boundary, which is established according to an actual chemical environment, continuously and randomly vibrating molecules in the box by using a molecular dynamics method, and continuously and randomly vibrating the molecules in the box during the period, and the system records the change of the total dipole moment in the box at each moment, and then converts a dipole moment signal into infrared frequency and intensity through Fourier transform, so that the infrared spectrum of the macromolecular bioprotein is obtained, and accurate analysis and rapid detection of the macromolecular biotoxin are realized. The difficulty that spectrum calculation of a large molecular weight system cannot be carried out due to the serious scale problem of a first principle and a density functional theory method is overcome, and a complete route of infrared spectrum dynamic properties of the system is obtained by adopting a molecular dynamics calculation method; therefore, infrared spectrum prediction of large-scale biological protein molecules is realized through a calculation means.

The utility model relates to the technical field of detection cards, and discloses a biotoxin detection card which comprises a shell and detection test paper, the shell comprises a bottom shell and a shell cover detachably installed on the bottom shell, the upper side face of the bottom shell is concaved inwards to form a driving groove and a containing groove which are communicated, and the containing groove is used for containing the detection test paper. A limiting piece for limiting the test paper in the placing groove is arranged in the driving groove in a sliding manner; the detection test paper comprises a bottom plate, a sample pad, a colloidal gold pad, a nitrocellulose membrane and an absorption pad are sequentially arranged on the bottom plate, a detection line is arranged on the nitrocellulose membrane, a sample adding hole corresponding to the sample pad is formed in the shell cover, and an observation opening corresponding to the nitrocellulose membrane is formed in the shell cover. The shell cover is detachably mounted on the bottom shell, meanwhile, the limiting piece moves in the driving groove, and the detection test paper is limited in the containing groove, so that the detection test paper can be better taken and placed, namely, the detection test paper of the detection card can be replaced, and the shell is repeatedly used.

The invention discloses an ochratoxin A hapten, an ochratoxin A artificial antigen, an ochratoxin A antibody and a preparation method and application thereof, and relates to the technical field of immunochemistry and biotoxin detection. The hapten is prepared from ochratoxin A and succinic anhydride through reaction, the artificial antigen is prepared from the hapten and isobutyl chloroformate, and the antibody is prepared from animals immunized by the artificial antigen. The antigen and the antibody can be applied to preparation of an ochratoxin A colloidal gold immunochromatography test strip, and can rapidly and sensitively detect ochratoxin A residues in animal-derived food.

The present invention discloses a biological toxin detection processor for meat food, which relates to the technical field of food detection devices. It includes a processing box. An ultrasonic extractor is arranged inside the processing box. A vibrating rod is installed at the output end of the ultrasonic extractor. Inside the processing box, a collection bottle body, a test tube and a base are arranged in sequence from top to bottom. A discharge pipe is connected to the lower end of the collection bottle body. A top cover is installed at the upper end of the collection bottle body. A screw blade is arranged inside the collection bottle body. A plurality of triangular pieces are arranged on the inner wall of the test tube. A lifting component and a translation component are installed on the outer side of the base; the translation component is used to push the base to slide directly below the vibrating rod, and the lifting component is used to raise the position of the base, so that the lower end of the vibrating rod penetrates into the test tube to perform ultrasonic oscillation work. After the extraction work is completed, the position of the base is lowered to separate the test tube from the vibrating rod, thereby achieving the effect of continuously processing meat food and avoiding contamination of the sample due to frequent transfer.

The utility model discloses biotoxin detection equipment convenient to flexibly move and use, which comprises a detector body, an auxiliary moving mechanism is arranged at the rear end of the detector body, two sides of the auxiliary moving mechanism are rotatably connected with rollers, the auxiliary moving mechanism is clamped at the rear end of the detector body, and the rollers are rotatably connected with the auxiliary moving mechanism. A connecting plate is arranged above the rear end of the detector body, the middle part of the connecting plate is in threaded connection with an adjusting screw rod, and the bottom end of the adjusting screw rod is rotationally connected with the auxiliary moving mechanism; a pull rod is inserted into the bottom of the front end of the detector body, and the pull rod is telescopically connected with the detector body. According to the utility model, the auxiliary moving mechanism is arranged at the rear end of the detector body and can be adjusted up and down according to use requirements; a pull rod is arranged at the front end of the detector body, and the whole detector body can flexibly move through cooperation of the pull rod and the auxiliary moving mechanism, so that the whole detector can be conveniently moved to a specified position according to use requirements.

Provided is a multi-biotoxin detection chip, comprising a PDMS chip layer and a microelectrode layer bonded to each other, wherein the PDMS chip layer is provided with an injection hole and at least two sample outlet holes, and its bonding surface is provided with pits and connection grooves between the injection hole and each sample outlet hole, which are connected in sequence; after bonding with the microelectrode layer, each connection groove forms a flow channel, and each pit forms a detection cavity; on the bonding surface of the microelectrode layer, each thin layer detection electrode unit respectively arranged in the bonding corresponding area of ​​each detection cavity comprises a working electrode and a counter electrode; wherein the working electrode is a BDD thin film electrode generated by boron-doped diamond, and each working electrode is respectively modified with a nucleic acid aptamer corresponding to the target biotoxin to be detected by the working electrode; the counter electrode is a platinum thin layer electrode formed by platinum 1000 through a sputtering process; the invention also provides a manufacturing method and a detection method of such a chip; the invention can realize high-sensitivity rapid detection of multiple indicators of biotoxins.

The application discloses a non-toxic genetically engineered toxoid molecule antigen vaccine based on a protective antigen functional domain structure and a preparation method and application thereof. It is determined that botulinum toxin and tetanus toxin both have two key protective antigen molecules, i.e. a receptor binding region Hc and a light chain-transmembrane region L-HN. Based on the important role of the functional domain in immunoprotective efficacy, a non-toxic genetically engineered toxoid or chimeric toxoid multivalent vaccine molecule is designed and prepared by using a biosynthesis technology. The vaccine has two important protective antigens of biological toxins, can produce strong complete protection at a low dose and a small number of immunization times, and can be used as a high-efficiency genetically engineered toxoid vaccine to replace formaldehyde inactivated toxoids for biological toxin prevention. The vaccine has important protective antigens of two different biological toxins, can produce protective efficacy against a plurality of different toxin biological agents, and can be used as a broad-spectrum multivalent vaccine for biological toxin prevention.

The present invention discloses a method for drying berries, which first uses a combined treatment of pulsed electric field and plasma on the berries, and then dries the berries after the combined treatment. This method can change the microstructure inside the berries, including increasing the surface roughness, increasing hydrophilicity, forming surface electroporation holes and internal membrane perforations, thereby promoting water migration, improving the drying efficiency and quality of the berries. This method also has the advantages of no residue and simple operation. By using a combined treatment of pulsed electric field and plasma to treat the berries, it can inactivate the microorganisms on the berries, degrade the biotoxins produced by the microorganisms, reduce the loss of nutrients caused by drying, and at the same time can inactivate oxidase and reduce the oxidation of antioxidant substances during the drying process.

This invention proposes a method for synthesizing T-2 toxin antigen and preparing and applying monoclonal antibodies, relating to the field of biotoxin detection technology. The method for preparing T-2 toxin antigen includes the following steps: First, the immunogen T-2-BSA is synthesized using the carbodiimide method. 2 mg of T-2 adipic acid is dissolved in 0.5 mL of dimethylformamide, denoted as solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved in a 2-morpholine ethanesulfonic acid buffer solution at pH 5.5, denoted as solution B. Second, solution A is added dropwise to solution B under stirring. The mixture is stirred at 4°C in the dark for 18 hours, then dialyzed with phosphate buffer solution for 3 days, changing the solution daily. The dialysate is centrifuged at 3500 r / min for 10 min, and the precipitate is discarded. The unique step of chemically conjugating adipic acid to T-2 toxin not only enriches the structural characteristics of the hapten, but also results in a complete T-2 toxin antigen that exhibits excellent immunogenicity, efficiently stimulating the body's immune response and demonstrating high specificity and sensitivity.

The utility model relates to the technical field of specimen extraction, in particular to a specimen extraction device for food biotoxin detection, which comprises a food biotoxin specimen culture box, a plurality of through grooves are formed in the food biotoxin specimen culture box, and a same-column extraction structure is slidably mounted in each through groove. The same-column taking-out structure comprises sliding columns, each sliding column is partially exposed below the food biotoxin specimen culture box, the number of the sliding columns is five, a first connecting rod is fixedly installed between every two columns of sliding columns, and by arranging the same-column taking-out structure, a worker can lift up a handle on the side wall of a certain column of sliding columns to take out the food biotoxin specimen culture box; the handle generates a radial force to lift up the whole column of sliding columns through the handle rod, and the column of sliding columns slide upwards in the through groove of the food biotoxin specimen culture box, so that a worker can conveniently take out the specimen box subsequently; and the problem that the specimen boxes are inconvenient to take out by workers due to the fact that the specimen boxes are pushed together is solved.

The present invention relates to the technical field of biological toxin detection, and discloses a system and method for intelligent interpretation of a six-in-one mycotoxin detection card based on multidimensional data analysis. The method comprises collecting first toxin detection data of six mycotoxins, including fluorescence response time series, colloidal gold response time series and environmental variable time series, and performing feature extraction to obtain second toxin detection data; then establishing a historical detection database, constructing and training a mycotoxin classification model, and obtaining preliminary identification results of the six mycotoxins based on the second toxin detection data and the classification model; finally, weighted fusion of the preliminary identification results is performed in combination with the environmental variable time series to obtain accurate identification results; the present invention realizes intelligent interpretation of mycotoxin detection and greatly improves detection accuracy and efficiency.

The utility model relates to the technical field of food detection, and discloses a cleaning device for detecting biotoxin in food, which comprises a cleaning box, the right end of the cleaning box is fixedly connected with a motor, the driving end of the motor penetrates through the cleaning box and is fixedly connected with a rotating column, and one end of the rotating column far away from the motor is fixedly connected with a filter cartridge. Fixing rings are rotatably connected to the outer walls of the left end and the right end of the filter cartridge, supporting blocks are fixedly connected to the front ends and the rear ends of the fixing rings on the two sides, and the ends, away from the fixing rings, of the supporting blocks on the two sides are fixedly connected to the inner wall of the cleaning box. According to the filter, the rotating block, the connecting rod and the limiting block rotate in the through groove, the limiting block is separated from the limiting groove, and the spring and the pressing plate are matched, so that the barrel cover and the filter barrel are convenient to disassemble, the workload of operators is reduced, and the effect of improving the cleaning efficiency is achieved.

The utility model belongs to the field of biotoxin detection, particularly relates to a rapid extraction device for biotoxin in food, and aims to solve the problem that after a piston head is pulled for extraction in the prior art, the piston head is not relatively fixed, so that the piston head is squeezed during taking and moving, and an extract is squeezed out. The device comprises a device body, the device body comprises a sleeve and a needle head, the needle head is fixedly communicated with the bottom end of the sleeve, a piston head is arranged in the sleeve in a sliding mode, and a piston pull rod is fixedly arranged at the top end of the piston head. When a sample is extracted, the pressing block can be extruded to relieve the fixed state of the protective shell and the pull plate, then the needle head can be inserted into the sample, the pull plate is pulled to extract, then the arc-shaped ring is rotated to the original position to be fixed again, the operation is simple and convenient, and the effect of rapid extraction is achieved.

The invention discloses a multi-mode ion source combination device and a detection method thereof.The multi-mode ion source combination device comprises a fixed shell, a heating module, two probe sample introduction modules, an ESI module, a voltage module and a control unit, the two probe sample introduction modules are located above and on the left side of the fixed shell respectively, the heating module is installed above the fixed shell, and the ESI module is installed on the left side of the fixed shell; the ESI module is installed on the left side of the fixed shell, the fixed shell is matched with a triple quadrupole mass spectrometer interface, and the control unit can control equipment to be switched in situ between a TD-ESI mode and a nano-ESI mode. The equipment does not need a solid-liquid conversion process, the detection speed is less than or equal to 1min, a 100-1000Da full molecular weight domain is covered, no sample transfer loss exists, complicated trace material evidences such as explosion residues, drug metabolites and biotoxins can be rapidly detected, the technical problems of difficulty in sampling, narrow range and low efficiency of an existing ion source are solved, and the equipment is suitable for on-site evidence collection and detection of public safety.

To provide devices for viral, microbial and pathogen control and removal.SOLUTION: A device comprises a carrier material comprising a carbohydrate-based polymer, and a binding agent. The binding agent is attached by one or more covalent bonds to the carrier material, and the binding agent can bind to a target, the target being one or more of a biotoxin, a virus, a microbe, and a microbial component.SELECTED DRAWING: Figure 3A

The present invention relates to the field of pharmaceutical engineering technology, and specifically to a drug assembly and a preparation method and application thereof. The drug assembly provided by the present invention is obtained by self-assembly of a protein polypeptide calcium channel blocking drug and an amide local anesthetic; the protein polypeptide calcium channel blocking drug includes conotoxin or its derivatives. The present invention can not only quickly block nerve conduction to achieve rapid analgesia, but also prolong the analgesic time, and minimize systemic toxicity. The drug preparation can maintain long-term analgesia, providing a powerful tool for the use of biological toxin molecular sustained / controlled release preparations as non-addictive drugs to control severe pain.

The application belongs to the field of analysis and detection, and particularly relates to preparation of a bifunctional imprinting fluorescent probe and sensitive detection of marine biological toxins and antiviral drugs. The application adopts valganciclovir hydrochloride (VAL) similar in structure to saxitoxin (STX) as a pseudo-template, avoids the high toxicity of saxitoxin (STX) and the risk of template leakage, adopts a primary-secondary functional monomer synergistic strategy to construct a high-specificity recognition site, combines a fluorescent dye occupation-competition release mechanism to realize fluorescence signal transduction, and prepares an imprinting polymer fluorescent probe which has high selectivity, high sensitivity and fast response speed for STX and VAL, and can simultaneously adapt to the detection requirements of marine environmental samples and clinical biological samples, and still maintains excellent recovery rate and stability in a complex matrix.