17 results about "Biological toxin" patented technology

The invention relates to the technical field of vaccines, and discloses a preparation method and application of an anti-tumor vaccine based on an autoantigen and a synthetic antigen. The anti-tumor vaccine comprises a tumor autoantigen and a synthetic antigen, wherein the tumor autoantigen is extracted from a tumor cell membrane, a tumor cell lysate, a tumor cell secretion, an exosome antigen or an antigen released by a tumor microenvironment; the synthetic antigen is selected from at least one of a tumor associated antigen, a tumor specific antigen, a personalized neoantigen, a pathogen antigen, a biotoxin, a biomolecule antigen and coding nucleic acid of a peptide type antigen in the antigens; the coding nucleic acid is DNA or mRNA. The vaccine molecule composed of the tumor autoantigen and the synthetic antigen is easy to prepare, has a strong anti-tumor immune effect and good biological safety, solves the problems that the traditional tumor autoantigen has no specific precise target spot and the synthetic antigen is single in immunogen, and has a wide application prospect.

The invention provides synthesis of a T-2 toxin antigen and preparation and application of a monoclonal antibody. Relates to the technical field of biotoxin detection. The preparation method of the T-2 toxin antigen comprises the following steps: step 1, synthesizing an immunogen T-2-BSA by adopting a carbodiimide method, dissolving 2mg of T-2 adipic acid with 0.5 mL of dimethylformamide, and recording as a solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved with a 2-morpholine ethanesulfonic acid buffer solution with the pH being 5.5, and the solution is marked as a solution B; step 2, under the condition of stirring, dropwise adding the solution A into the solution B, stirring for 18 hours in a dark place at 4 DEG C, then dialyzing for 3 days by using a phosphate buffer solution, changing the solution once a day, centrifuging the dialysate for 10 minutes at 3,500 r / min, and discarding the precipitate; the adipic acid is coupled with the T-2 toxin through a chemical method, and the unique step enriches the structural characteristics of the hapten, so that the prepared T-2 toxin complete antigen is excellent in immune effect, can efficiently excite immune response of an organism, and shows high specificity and sensitivity.

The invention relates to the technical field of intelligent medical first aid and data processing, in particular to an intelligent first aid information studying and judging platform for war wounds and biological injuries. The system comprises a data acquisition module, a feature reconstruction module, an entropy research and judgment module and a decision generation module. The system executes causal inference decoupling based on the composite physiological data and the environmental parameters; the method is characterized in that dominant trauma and recessive biological injury characteristics are separated, study and judgment confidence and user execution entropy are calculated, and an emergency treatment strategy is determined according to the game relationship of the two; according to the method, the non-linear physiological masking effect caused by biological factors is effectively stripped, the problem of misjudgment caused by the fact that biological toxins cover wound signs is solved, and acute capture and early warning of recessive biological injuries are achieved.

The invention discloses a multi-dimensional high-precision detection method and system for biotoxins in agricultural products, and the method comprises the steps: carrying out sterile collection and grading pretreatment on an agricultural product sample to obtain a homogenized sample; extracting the homogenized sample by adopting a mixed solvent system, adding an internal standard substance, carrying out salting-out layering, and collecting supernate; sequentially carrying out QuEChERS purification, multi-mechanism adsorption column purification and molecularly imprinted polymer specific enrichment on the supernate; carrying out dual-mode detection on the target toxin enrichment liquid, respectively carrying out quantitative detection by adopting a liquid chromatography-tandem mass spectrometry method, and carrying out screening detection by adopting an intelligent electrochemical sensing method; performing optimization processing on the dual-mode detection signal, and comprehensively judging a sample detection result; through integrated application of a triple purification mechanism, a dual-mode detection system, machine learning signal analysis and a full-chain quality control technology, efficient enrichment and deep interference removal of target toxins in a complex matrix can be realized, and both precision and applicability are considered.

The application discloses a labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection, and the labeling method comprises the following steps: S1, double-fluorescent microsphere preparation: S1.1, performing carboxyl site blocking on aflatoxin B1, vomitoxin and zearalenone antibodies; S1.2, performing coupling on amino sites of the closed aflatoxin B1, vomitoxin and zearalenone antibodies; S1.3, activating the coupled aflatoxin B1, vomitoxin and zearalenone antibodies; and S1.4, coupling the activated aflatoxin B1, vomitoxin and zearalenone antibodies with magnetic fluorescent microspheres. The labeling method capable of improving fluorescence amplification effect and application thereof in biological toxin detection have the advantages that the sensitivity and detection limit of products can be improved, and the detection difference caused by the interference of sample matrix can be reduced.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The utility model discloses biotoxin detection equipment convenient to flexibly move and use, which comprises a detector body, an auxiliary moving mechanism is arranged at the rear end of the detector body, two sides of the auxiliary moving mechanism are rotatably connected with rollers, the auxiliary moving mechanism is clamped at the rear end of the detector body, and the rollers are rotatably connected with the auxiliary moving mechanism. A connecting plate is arranged above the rear end of the detector body, the middle part of the connecting plate is in threaded connection with an adjusting screw rod, and the bottom end of the adjusting screw rod is rotationally connected with the auxiliary moving mechanism; a pull rod is inserted into the bottom of the front end of the detector body, and the pull rod is telescopically connected with the detector body. According to the utility model, the auxiliary moving mechanism is arranged at the rear end of the detector body and can be adjusted up and down according to use requirements; a pull rod is arranged at the front end of the detector body, and the whole detector body can flexibly move through cooperation of the pull rod and the auxiliary moving mechanism, so that the whole detector can be conveniently moved to a specified position according to use requirements.

The application discloses a non-toxic genetically engineered toxoid molecule antigen vaccine based on a protective antigen functional domain structure and a preparation method and application thereof. It is determined that botulinum toxin and tetanus toxin both have two key protective antigen molecules, i.e. a receptor binding region Hc and a light chain-transmembrane region L-HN. Based on the important role of the functional domain in immunoprotective efficacy, a non-toxic genetically engineered toxoid or chimeric toxoid multivalent vaccine molecule is designed and prepared by using a biosynthesis technology. The vaccine has two important protective antigens of biological toxins, can produce strong complete protection at a low dose and a small number of immunization times, and can be used as a high-efficiency genetically engineered toxoid vaccine to replace formaldehyde inactivated toxoids for biological toxin prevention. The vaccine has important protective antigens of two different biological toxins, can produce protective efficacy against a plurality of different toxin biological agents, and can be used as a broad-spectrum multivalent vaccine for biological toxin prevention.

This invention proposes a method for synthesizing T-2 toxin antigen and preparing and applying monoclonal antibodies, relating to the field of biotoxin detection technology. The method for preparing T-2 toxin antigen includes the following steps: First, the immunogen T-2-BSA is synthesized using the carbodiimide method. 2 mg of T-2 adipic acid is dissolved in 0.5 mL of dimethylformamide, denoted as solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved in a 2-morpholine ethanesulfonic acid buffer solution at pH 5.5, denoted as solution B. Second, solution A is added dropwise to solution B under stirring. The mixture is stirred at 4°C in the dark for 18 hours, then dialyzed with phosphate buffer solution for 3 days, changing the solution daily. The dialysate is centrifuged at 3500 r / min for 10 min, and the precipitate is discarded. The unique step of chemically conjugating adipic acid to T-2 toxin not only enriches the structural characteristics of the hapten, but also results in a complete T-2 toxin antigen that exhibits excellent immunogenicity, efficiently stimulating the body's immune response and demonstrating high specificity and sensitivity.

The utility model relates to the technical field of specimen extraction, in particular to a specimen extraction device for food biotoxin detection, which comprises a food biotoxin specimen culture box, a plurality of through grooves are formed in the food biotoxin specimen culture box, and a same-column extraction structure is slidably mounted in each through groove. The same-column taking-out structure comprises sliding columns, each sliding column is partially exposed below the food biotoxin specimen culture box, the number of the sliding columns is five, a first connecting rod is fixedly installed between every two columns of sliding columns, and by arranging the same-column taking-out structure, a worker can lift up a handle on the side wall of a certain column of sliding columns to take out the food biotoxin specimen culture box; the handle generates a radial force to lift up the whole column of sliding columns through the handle rod, and the column of sliding columns slide upwards in the through groove of the food biotoxin specimen culture box, so that a worker can conveniently take out the specimen box subsequently; and the problem that the specimen boxes are inconvenient to take out by workers due to the fact that the specimen boxes are pushed together is solved.

The invention discloses a multi-mode ion source combination device and a detection method thereof.The multi-mode ion source combination device comprises a fixed shell, a heating module, two probe sample introduction modules, an ESI module, a voltage module and a control unit, the two probe sample introduction modules are located above and on the left side of the fixed shell respectively, the heating module is installed above the fixed shell, and the ESI module is installed on the left side of the fixed shell; the ESI module is installed on the left side of the fixed shell, the fixed shell is matched with a triple quadrupole mass spectrometer interface, and the control unit can control equipment to be switched in situ between a TD-ESI mode and a nano-ESI mode. The equipment does not need a solid-liquid conversion process, the detection speed is less than or equal to 1min, a 100-1000Da full molecular weight domain is covered, no sample transfer loss exists, complicated trace material evidences such as explosion residues, drug metabolites and biotoxins can be rapidly detected, the technical problems of difficulty in sampling, narrow range and low efficiency of an existing ion source are solved, and the equipment is suitable for on-site evidence collection and detection of public safety.

The application belongs to the field of analysis and detection, and particularly relates to preparation of a bifunctional imprinting fluorescent probe and sensitive detection of marine biological toxins and antiviral drugs. The application adopts valganciclovir hydrochloride (VAL) similar in structure to saxitoxin (STX) as a pseudo-template, avoids the high toxicity of saxitoxin (STX) and the risk of template leakage, adopts a primary-secondary functional monomer synergistic strategy to construct a high-specificity recognition site, combines a fluorescent dye occupation-competition release mechanism to realize fluorescence signal transduction, and prepares an imprinting polymer fluorescent probe which has high selectivity, high sensitivity and fast response speed for STX and VAL, and can simultaneously adapt to the detection requirements of marine environmental samples and clinical biological samples, and still maintains excellent recovery rate and stability in a complex matrix.

The utility model belongs to the technical field of biotoxin detection, and particularly relates to a dropper for detecting horseshoe crab recombinant factor C high-sensitivity endotoxin. The dropper comprises a sampling tube and a rubber cap, the rubber cap is assembled at the top of the sampling tube, an overflow tube is fixedly connected to the side wall of the sampling tube, and a collecting assembly is assembled at the other end of the overflow tube. A one-way valve is assembled on the inner wall of the overflow pipe, and a first guide plate and a second guide plate are fixedly connected to the joint of the inner wall of the sampling pipe and the overflow pipe. The collecting assembly comprises a collecting pipe assembled at one end of the overflow pipe, a sealing cover is assembled at one end of the collecting pipe, baffles are fixedly connected to the side wall of the overflow pipe and the side wall of the collecting pipe respectively, and a rubber sleeve is connected between the two baffles. When the dropper is used for selecting solutions, redundant solutions can be guided out and collected, and the phenomenon that the solutions enter the rubber cap is effectively avoided.

The application provides a composite material based on platinum nanoparticle loaded nickel-cobalt double metal hydroxide, an electrochemical sensor and a method for detecting biological toxins, and belongs to the technical field of fungal toxin detection. The composite material comprises an electric signal amplification material, and the surface of the electric signal amplification material is modified with a nucleic acid aptamer; the electric signal amplification material comprises a carrier and an active component; the carrier is double metal hydroxide, and the double metal hydroxide is nickel-cobalt hydroxide; and the active metal element in the active component is at least one selected from Pt and Au. The electrochemical sensor constructed by the application shows the advantages of simple operation, high sensitivity and strong specificity in OTA detection, and simultaneously provides a path for the rapid detection of other biological toxins.

The application discloses a gene modified non-toxic toxoid vaccine based on light chain-transmembrane region L-HN and receptor binding region Hc, and a preparation method and application thereof. The gene modified non-toxic novel toxoid vaccine molecule has two important protective antigen functional domains of a biological toxin, the N terminal of the vaccine molecule is the light chain-transmembrane region L-HN, and the C terminal is the receptor binding region Hc. The two protective antigen functional domains can be derived from the same biological toxin, can produce stronger protection than a single antigen, can produce strong complete protection at a low dose and a small number of immunization times, and can be used as a high-efficiency gene engineering toxoid vaccine to replace formaldehyde inactivated toxoid for biological toxin prevention. The two protective antigen functional domains can also be derived from different biological toxins, can protect against attacks of respective biological toxins, can produce protection against multiple different toxin biological agents, and can be used as a broad-spectrum multivalent vaccine for biological toxin prevention.

The present application relates to the technical field of intelligent medical first aid and data processing, in particular to an intelligent first aid information research and judgment platform for war trauma combined with biological injury; comprising data acquisition, feature reconstruction, entropy value research and decision generation modules; the system performs causal inference decoupling based on composite physiological data and environmental parameters; the core is to separate the characteristics of overt trauma and latent biological injury, calculate the confidence and user execution entropy, and determine the first aid treatment strategy according to the game relationship between the two; the present application effectively removes the nonlinear physiological masking effect caused by biological factors, solves the misjudgment problem caused by biological toxins covering up trauma signs, and realizes the sensitive capture and early warning of latent biological injury.