32 results about "Fumonisin" patented technology

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumDPS mutants T194V, T194V-P40C / Y173C, T194V-N43C / D83C and T194V-F71C / S117C related to the invention is obviously improved compared with that of the wild type fumonisins degrading enzyme, and the thermal stability of the fumonisins degrading enzyme FumDPS mutants is obviously improved. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is convenient to transport and store, so that the investment of industrial cost can be reduced.

The present disclosure concerns polypeptide having fumonisin esterase activity exhibiting increased fumonisin esterase activity, when measured at a temperature of at least 37° C., when compared to the control polypeptide comprising the amino acid of SEQ ID NO: 3. The polypeptides of the present disclosure include one or more amino acid variations which contribute to the increase fumonisin esterase activity. The present disclosure also includes compositions comprising the polypeptide having fumonisin esterase activity, methods for detoxifying a fumonisin mycotoxin as well as processes for making the compositions comprising the polypeptide having fumonisin esterase activity.

The present application relates to a novel fumonisin-degrading enzyme and uses thereof.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The present invention relates to fusarium toxin cleavage polypeptide variants of a fusarium toxin carboxylesterase of SEQ ID No. 46 wherein said polypeptide variants each have an amino acid sequence shortened by 47 amino acids at the N-terminus and said amino acid sequence has a sequence identity of at least 70%, preferably 80%, particularly preferably 100%, to the amino acid sequence segments 48-540 of SEQ ID No. 46, i.e. SEQ ID No. 1; and isolated polynucleotides encoding the polypeptide variants; and an additive for cleaving fusarium toxins comprising at least one polypeptide variant and optionally at least one adjuvant; the invention also relates to the use of said polypeptide variants or additives for hydrolytically cleaving at least one fusarium toxin, and to a method for hydrolytically cleaving at least one fusarium toxin.

An enzyme includes an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1, for fumonisin degradation in a sample. A method for fumonisin degradation is based on the incubation of the contaminated sample with an enzyme comprising an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The present invention relates to a method for improving the enzyme kinetics of a fumonisins esterase, to an improved fumonisins esterase and the use thereof, to a composition comprising said fumonisins esterase and to a method for modifying fumonisins.

The invention refers to a fumonisin-sensitive yeast, specifically Saccharomycescerevisiae, comprising disrupted or deleted genes SNQ2, PDR12, YOR1, CKA2, LCB3, and optionally any one of VPS51, VPS52, or VPS53 and its use for detecting inhibitory activity of fumonisins or detecting fumonisin detoxifying compounds in a sample. The fumonisin-sensitive yeast is also useful for screening of fumonisin detoxification or resistance conferring activity of heterologous polypeptides.

The invention relates to a fumonisins degrading enzyme with improved activity and application of the fumonisins degrading enzyme.

The present invention relates to the use of an animal feed additive that reduces the toxic effects of mycotoxins, especially fumonisin B1 and zearalenone. This additive is a mycotoxin adsorbent consisting of an organically modified phyllosilicate with 71% substitution of its cation exchange capacity with the organic compound dioctadecyldimethylammonium chloride.

The application relates to a traditional Chinese medicine and additive compound preparation for treating gizzarditis of laying hens and application, and relates to the technical field of veterinary drugs. The compound preparation is prepared by mixing a traditional Chinese medicine extract and a functional feed additive. The raw material composition of the traditional Chinese medicine extract comprises torrefied malt, torrefied medicated leaven, radix codonopsis, rhizoma atractylodis macrocephalae, rhizoma coptidis, andrographis, dried tangerine or orange peel, white arillus, and liquorice. The additive comprises neutral protease, a mould inhibitor and probiotics. The mould inhibitor is selected from aspergillus flavus B1 degrading enzyme, corn strobukenone degrading enzyme, fumonisin degrading enzyme and vomitoxin degrading enzyme. The probiotics are selected from lactobacillus plantarum, bacillus subtilis and fructo-oligosaccharide. The application is based on the traditional Chinese veterinary theory of food digestion and spleen health, dampness expelling and qi regulation, heat clearing and toxin resolving and mucosa repairing. In combination with the effects of the additive on digestion, mould expelling and intestinal micro-ecological regulation, the application has a remarkable treatment effect on gizzarditis of laying hens, can improve the feed intake and weight gain, reduce the gizzard index, promote the repair of gastric mucosa, and has no antibiotic residue and no toxic side effect.

The invention belongs to the field of crop disease resistance identification. The invention discloses a method for accurately evaluating the resistance of a corn variety to fusarium verticillium ear rot. The method comprises the following steps: inoculating corn ears by adopting a needling inoculation method, harvesting, threshing and grinding the ears in a harvesting period, measuring the content of fumonisins in grains, and evaluating the resistance of a corn variety to fusarium verticillium ear rot through the content of fumonisins in the grains. According to the method, the resistance of the corn variety to the fusarium verticillium ear rot can be evaluated more accurately, and the corn variety harmful to human and livestock is eliminated, so that the food safety is guaranteed.

The invention relates to a natural composite trace element modified mycotoxin adsorbent and a preparation method thereof, the natural composite trace element modified mycotoxin adsorbent comprises the following raw materials by weight: 80-90 parts of natural rare earth zeolite eutectic ore, 5-10 parts of composite trace elements, 3-5 parts of an active ion modifier, and 1-2 parts of a dispersant; the natural rare earth zeolite eutectic ore originates from a Bayan Obo mining area, is high-purity crystallized hydrated sodium aluminosilicate calcium, and contains a zeolite and montmorillonite eutectic structure. Through synergistic modification of the natural mineral carrier and the compound trace elements, broad-spectrum efficient adsorption of various mycotoxins such as aflatoxin, fumonisins and zearalenone is achieved, and the adsorption stability in the full pH range of a digestive system is excellent; meanwhile, the feed has the multifunctional characteristics of resisting bacteria, removing heavy metals and promoting animal growth, does not influence absorption of nutritional ingredients such as vitamins and mineral substances in the feed, is nontoxic and harmless, is not accumulated in animal bodies, is simple and controllable in preparation process and low in cost, and is suitable for the fields of livestock and poultry and aquaculture.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of amine oxidase ACAO and a mutant. Compared with a wild type enzyme, the temperature tolerance of the mutant enzyme in a certain temperature range is superior to that of the wild type enzyme, and the thermal stability is remarkably improved. Through molecular modification on a wild type, the thermal stability of amine oxidase ACAO mutant enzyme is remarkably improved. The enzyme is mainly applied to the industries of food production, livestock feed processing and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The thermal stability improved amine oxidase ACAO mutant obtained by the invention is beneficial to improving the production efficiency of continuous production of enzyme in actual industrial application, has good industrial application prospects, and lays a foundation for further research on the structure of amine oxidase.

The invention discloses lazy bacillus for degrading fumonisins, a fumonisins degrading enzyme and application of the fumonisins degrading enzyme. The invention firstly discloses lazobium sp. And application of the lazobium sp. In degradation or removal of fumonisins. The invention further discloses a fumonisins degrading enzyme or a composition thereof and application of the fumonisins degrading enzyme or the composition thereof in degradation or removal of fumonisins. According to the lazelibacter ASAG15 and the three fumonisins degrading enzymes which are excavated from the lazelibacter ASAG15 and have good fumonisins degrading or removing effects, degradation products of the fumonisins can be further degraded or removed through combined use, and the lazelibacter ASAG15 and the three fumonisins degrading enzymes can be applied to industries such as agriculture, feed and food and have wide application prospects. The method has a good application prospect in the fields of degradation, removal, prevention and control of fumonisins.

The utility model relates to the technical field of test strips, and discloses a chromatography type fumonisins test strip which comprises a main body shell, a limiting groove is formed in the side wall of an inner cavity of the main body shell, a filter is fixedly connected to the side wall of the inner cavity of the main body shell, a first filtering hole is formed in the side face of the filter, and a second filtering hole is formed in the side face of the filter. A filter block is fixedly connected to an inner cavity of the main body shell, second filter holes are formed in the side face of the filter block, a liquid retention cavity is formed in the side face of the filter block, a liquid inlet is formed in the side face of the liquid retention cavity, and a liquid outlet pipe is fixedly connected to the side face of the liquid inlet. According to the device, grains such as corn and the like can be directly ground and fully and uniformly mixed with a diluent, then the mixture is directly poured into a filter, an extrusion block is used for extruding, residues are filtered and separated, separated liquid can directly flow into a liquid outlet pipe and then is dropped into a sample adding hole, and the situation that the liquid needs to be dropped into a sample adding hole through a suction pipe after filtering is conveniently omitted.

The present invention relates to the use of an enzyme comprising an amino acid sequence having at least 80% sequence identity with sequence SEQ ID NO: 1 for degrading fumonisins in a sample. Furthermore, the present invention relates to a method of degrading fumonisins based on incubating a contaminated sample with an enzyme comprising an amino acid sequence having at least 80% sequence identity to the sequence SEQ ID NO: 1.

The invention belongs to the technical field of chemical synthesis. The invention provides a synthesis method of fumonisins series hidden toxins, and the synthesis method of the fumonisins series hidden toxins comprises the following steps: taking fumonisins as a raw material, and respectively carrying out synthesis of N-alkylated fumonisins, synthesis of hydrolyzed fumonisins and synthesis of N-amidated fumonisins. The invention also provides application of the fumonisins series hidden toxins synthesized by the synthesis method in preparation of toxin standard products. The fumonisins serve as raw materials, a series of fumonisins series hidden toxins are synthesized, the synthesis method is simple in step and efficient, and the purity of the synthesized fumonisins series hidden toxins is larger than 99%. The fumonisins series hidden toxins developed by the invention can be used for related detection, and have important significance on supervision and detection work in related food safety fields.

The invention relates to the technical field of agricultural biology, in particular to a nucleic acid aptamer for detoxification of fumonisins B1 and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer provided by the invention is shown as any one or more sequences of SEQ ID NO.1 to SEQ ID NO.3. The invention also provides a preparation method of the nucleic acid aptamer. The nucleic acid aptamer has the advantages of high efficiency, convenience in operation, no need of specific professional equipment, no influence on nutrient substances of agricultural and sideline products, no generation of harmful substances in the detoxification process and no need of multi-enzyme synergistic degradation in the detoxification aspect of the fumonisins B1, and also has the advantages of no requirement on environmental conditions and the like; and the detoxification of the fumonisins B1 can be completed at room temperature. The nucleic acid aptamer provided by the invention can overcome part of defects and limitations of the antibody, and is low in immunogenicity, easy in-vitro synthesis, simple in modification and the like. The nucleic acid aptamer can be applied to agriculture, feed and other industries, and harm of FB1 to animal and human health is reduced.

The application belongs to the technical field of protein engineering, and particularly relates to a fumonisin-degrading enzyme mutant with improved specific activity and thermal stability and application. The fumonisin-degrading enzyme FumDSB mutant A373R, A373R / S248P, A373R / Q259P, A373R / T282D, A373R / G321A, A373R / D330P and A373R / A378K of the application show significantly improved relative activity and thermal stability compared with wild-type fumonisin-degrading enzyme. The enzyme is mainly applied to the industries of agricultural products, animal feed, food and the like to reduce the health hazards of fumonisin to animals and humans. The fumonisin mutant with improved activity and thermal stability obtained by the application is conducive to reducing the cost of the enzyme in industrial application.

This invention discloses a strain of *Trichoderma viride* and its application in antibacterial and toxin degradation, belonging to the field of microbial technology. The *Trichoderma viride* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41619. The *Trichoderma viride* strain exhibits a significant inhibitory effect on the growth and infection of *Aspergillus flavus*, with an inhibitory effect superior to 4000 times dilution of tebuconazole. This *Trichoderma viride* strain can produce enzymes that degrade the cell walls of pathogenic fungi. Fungal cell walls are composed of cellulose, glucan, chitin, and glycoproteins. The chitinase and cellulase produced by *Trichoderma viride* can degrade the fungal cell walls, thereby inhibiting the growth, development, and infection of *Aspergillus flavus*. Furthermore, this invention also experimentally demonstrates that *Trichoderma viride* has a highly efficient degradation effect on aflatoxin and fumonisin.

This invention relates to polypeptide variants of lysing fusarium toxins by a carboxylesterase having SEQ ID No. 46, each of said polypeptide variants having an amino acid sequence shortened by 47 amino acids at the N-terminus, and said amino acid sequence having at least 70%, preferably 80%, particularly preferably 100% sequence identity with amino acid sequence segment 48-540 of SEQ ID No. 46, i.e., SEQ ID No. 1; and isolated polynucleotides encoding said polypeptide variants; and additives comprising at least one polypeptide variant and optionally at least one adjuvant for lysing fusarium toxins; and the use of said polypeptide variants or additives for hydrolyzing at least one fusarium toxin, and methods for hydrolyzing at least one fusarium toxin.

The present invention discloses a method for determining fumonisins and ochratoxins in cereals, comprising: adding cereal powder to a mixed isotope internal standard, then adding an extracting solution and vortexing and stirring for extraction, subjecting the extracting solution to low-temperature centrifugation, and after phase separation, removing a lower layer solution to obtain the extracting solution; the extracting solution is an acetonitrile-water solution containing aqueous ammonia. The method of the present invention adopts a low-temperature centrifugation induced phase separation integrated method to achieve a one-step operation process integrating target extraction, enrichment, purification and centrifugation, and by optimizing the extracting solution, fumonisins and ochratoxins of different polarity properties can be simultaneously transferred to the same small volume collection layer without the need for additional tedious manual operations, greatly improving the extraction, enrichment and purification efficiency, having the advantages of simple operation, short time consumption and low cost, and providing a new determination method for sample preparation and detection of fumonisins and ochratoxins in cereals.

The invention discloses a method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on a microbial nano-enzyme label and application, and the method can be used for simultaneously and accurately detecting various common mycotoxins, including aflatoxin B1, zearalenone and fumonisins B1, by utilizing the enhanced catalytic effect mediated by the microbial nano-enzyme label. The surface of Escherichia coli is coated with a SiO2 interlayer so as to improve the structural stability and the solution dispersity, and meanwhile, the surface of Escherichia coli is coated with a layer of catalytic shell composed of Au (at) Pt nanoparticles so as to generate excellent colorimetric and catalytic performance. According to the application, ESi-Au-Pt is introduced into a nano enzyme-ICA system, so that the sensitivity and accuracy of on-site analysis of a complex sample are remarkably improved, the limit of detection (LOD) of synchronous detection of target fungaltoxin is reduced to 6.1 / 1.6 / 5.4 pg / mL, the detection range spans 4-5 orders of magnitude, in addition, the established method shows excellent accuracy and stability in a complex matrix, and the method can be applied to the field analysis of fungaltoxin. The method shows a huge potential in the field-based real-time monitoring of small-molecule pollutants.

The present application belongs to the technical field of protein engineering, and particularly relates to a method for improving the thermal stability of fumonisin-degrading enzyme by forming disulfide bonds. The fumonisin-degrading enzyme FumD mutant according to the present application shows a significant improvement effect on the thermal stability compared with the wild-type fumonisin-degrading enzyme. The enzyme is mainly applied to the livestock feed processing and food production industries to reduce the health hazards of fumonisin to animals and humans. The fumonisin-degrading enzyme mutant with improved thermal stability obtained by the present application is beneficial to maintaining the activity of the enzyme at a higher temperature, adapting to a more high-temperature production environment, reducing the use amount and replacement frequency of the enzyme, and the improvement in stability facilitates transportation and storage, so that the industrial cost input can be reduced.

The application belongs to the technical field of biosensors, and particularly relates to a preparation method of a photocatalytic signal amplification electrochemical biosensor for detection of fumonisin B1 and application thereof. The synthetic composite material of graphdiyne-methylene blue-gold nanorod is used as a base material to generate an electrochemical signal; a single-stranded DNA is self-assembled to prepare a DNA tetrahedral nanostructure, the size of the tetrahedron is adjusted by controlling the number of bases, and the probe is controllably assembled on the electrode surface; the cadmium selenide quantum dots connected to the DNA composite structure are used as a signal transduction element to realize amplification of the amount of the fumonisin B1-induced electrochemical signal change by degrading the methylene blue. The electrochemical aptamer sensor constructed in the application is used for detection of fumonisin B1, has high sensitivity and good selectivity, the detection range thereof spans five orders of magnitude, and the detection limit is as low as 0.45fg / mL ‑1 , and remarkable effects are achieved.

The present invention provides a method for preparing an adsorbent for efficiently enriching multiple mycotoxins in cereals. The method adopts polydopamine and 1-carboxyethyl-3-butylimidazolium bromide dual-modified magnetic nanoparticles as a novel adsorbent to enrich multiple types of mycotoxins in cereals, such as aflatoxins, ochratoxins, fumonisins, zearalenone toxins, penicillin toxins, sterigmatocystin, and Class A and Class B trichothecenes toxins. The method reduces the co-adsorption of matrix components in the cereals, and provides a sample to be tested in which the target is enriched and interference is purified for subsequent analysis and detection.

The present application relates to a variant polypeptide having fumonisin degrading activity, a composition comprising the variant polypeptide, use of at least any one of the variant polypeptide and a host cell expressing same for detoxifying fumonisin present in food and / or feed, and a fumonisin degrading method using same.