39 results about "Fumonisin" patented technology

The invention discloses a fumonisins B1 fluorescent single particle intelligent detection method based on truncated aptamer mediated nucleic acid cascade amplification, and belongs to the field of biosensing detection. The invention successfully constructs a deep learning assisted fluorescent single-particle aptamer sensing detection method by taking a truncated aptamer with high affinity as a recognition element and AIE dye fluorescence coded microspheres with excellent luminescence property and good dispersibility as a signal marker, and can be used for high-sensitivity detection of FB1 in food. Compared with an existing reported FB1 detection method, the method has the advantages of being wide in linear range, high in sensitivity, low in sample consumption and the like, automatic, rapid and high-throughput recognition and counting detection of red particles in a fluorescence image can be achieved, and the analysis efficiency is greatly improved.

The invention discloses streptomyces allogeri and an application thereof in biological prevention and control of toxin-producing fungi. In particular to streptomyces allorgeus ZxBac193 CGMCC (China General Microbiological Culture Collection Center) No.32341 and an application thereof. The streptomyces allorgeus ZxBac193 shows a strong antagonistic effect on toxin-producing fungi such as aspergillus flavus, aspergillus ochraceus, fusarium graminearum, fusarium oxysporum, penicillium citrinum and penicillium islanigerum; the compound can inhibit the growth of aflatoxin producing fungi, ochratoxin producing fungi, deoxynivalenol producing fungi, zearalenone toxin producing fungi, fumonisins producing fungi, citrinin producing fungi and island penicillin producing fungi, and can be prepared into various biological control products aiming at mycotoxins, such as biopesticides, antifungal agents and the like. The compound is used for inhibiting toxic fungi in the environment and preventing or relieving mycotoxin pollution, and has good development and application prospects in the fields of prevention and treatment of fungal pollution of food crops and the like.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of fumonisins degrading enzyme by forming disulfide bonds. The thermal stability of the fumonisins degrading enzyme FumDPS mutants T194V, T194V-P40C / Y173C, T194V-N43C / D83C and T194V-F71C / S117C related to the invention is obviously improved compared with that of the wild type fumonisins degrading enzyme, and the thermal stability of the fumonisins degrading enzyme FumDPS mutants is obviously improved. The fumonisins enzyme is mainly applied to the industries of livestock feed processing, food production and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins degrading enzyme mutant with improved thermal stability is convenient to transport and store, so that the investment of industrial cost can be reduced.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a fumonisins degrading enzyme mutant with improved specific activity and thermal stability and application. According to the mutants of the fumonisins degrading enzyme FumDSB, which are provided by the invention, the relative activity and the thermal stability of the mutants A373R, A373R / S248P, A373R / Q259P, A373R / T282D, A373R / G321A, A373R / D330P and A373R / A378K are obviously improved compared with those of a wild type fumonisins degrading enzyme. The enzyme is mainly applied to the industries of agricultural products, animal feed, food and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The obtained fumonisins mutant with improved activity and thermal stability is beneficial to reducing the cost of the enzyme in industrial application.

The present disclosure concerns polypeptide having fumonisin esterase activity exhibiting increased fumonisin esterase activity, when measured at a temperature of at least 37° C., when compared to the control polypeptide comprising the amino acid of SEQ ID NO: 3. The polypeptides of the present disclosure include one or more amino acid variations which contribute to the increase fumonisin esterase activity. The present disclosure also includes compositions comprising the polypeptide having fumonisin esterase activity, methods for detoxifying a fumonisin mycotoxin as well as processes for making the compositions comprising the polypeptide having fumonisin esterase activity.

Polynucleotides and polypeptides conferring the ability to detoxify or metabolize the mycotoxin fumonisin are provided, as are constructs, compositions, and plants comprising the polynucleotides and polypeptides. Also provided are plants having the ability to detoxify fumonisin, methods of conferring fumonisin detoxification, methods of controlling fumonisin contamination, and kits and methods for identification of plants having the ability to detoxify fumonisin.

The present invention discloses an electrochemical immunosensor for detecting fumonisin and its preparation and application, and relates to the field of medicinal plant quality safety monitoring. The preparation method of the electrochemical immunosensor includes: (1) preparation of N-G@CS composite material; (2) preparation of N-G@PANI@CS modified electrode; (3) preparation of AuNPs / N-G@PANI@CS / GCE modified electrode; preparation of BSA / Anti-FB1 / AuNPs / N-G@PANI@CS / GCE electrochemical immunosensor. The BSA / Anti-FB1 / AuNPs / N-G@PANI@CS / GCE electrochemical immunosensor of the present invention can realize the highly sensitive and rapid detection of fumonisin (FB1), a marker of root rot of medicinal plants, with a lower detection limit and a wider detection range, and has good specificity and stability, and also has the advantages of simple operation and short detection time.

The present application relates to a novel fumonisin-degrading enzyme and uses thereof.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The present invention relates to fusarium toxin cleavage polypeptide variants of a fusarium toxin carboxylesterase of SEQ ID No. 46 wherein said polypeptide variants each have an amino acid sequence shortened by 47 amino acids at the N-terminus and said amino acid sequence has a sequence identity of at least 70%, preferably 80%, particularly preferably 100%, to the amino acid sequence segments 48-540 of SEQ ID No. 46, i.e. SEQ ID No. 1; and isolated polynucleotides encoding the polypeptide variants; and an additive for cleaving fusarium toxins comprising at least one polypeptide variant and optionally at least one adjuvant; the invention also relates to the use of said polypeptide variants or additives for hydrolytically cleaving at least one fusarium toxin, and to a method for hydrolytically cleaving at least one fusarium toxin.

An enzyme includes an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1, for fumonisin degradation in a sample. A method for fumonisin degradation is based on the incubation of the contaminated sample with an enzyme comprising an amino acid sequence with, at least, a 80% sequence identity to sequence SEQ ID NO: 1.

Polynucleotides and polypeptides conferring the ability to detoxify or metabolize the mycotoxin fumonisin are provided, as are constructs, compositions, and plants comprising the polynucleotides and polypeptides. Also provided are plants having the ability to detoxify fumonisin, methods of conferring fumonisin detoxification, methods of controlling fumonisin contamination, and kits and methods for identification of plants having the ability to detoxify fumonisin.

The application provides application of neutral protease in degradation of fumonisin, and belongs to the technical field of biology, and it is verified through experiments that the neutral protease has good degradation effect on FB1. The application also provides a compound enzyme preparation which comprises the following components: 1-5% neutral protease, 5-20% montmorillonite, 75-94% soluble starch, and only by adding 0.5wt%-1.5wt% of the compound enzyme preparation, the efficient degradation of FB1 in common contaminated feed can be realized; specifically, the degradation rate of the compound enzyme preparation on FB1 in corn flour can reach 95.73%, the degradation rate of the compound enzyme preparation on FB1 in DDGS can reach 96.43%, the degradation rate of the compound enzyme preparation on FB1 in corn steep liquor can reach 64.74%, and the degradation rate of the compound enzyme preparation on FB1 in corn cob powder can reach 93.74%.

The present invention relates to a method for improving the enzyme kinetics of a fumonisins esterase, to an improved fumonisins esterase and the use thereof, to a composition comprising said fumonisins esterase and to a method for modifying fumonisins.

The invention refers to a fumonisin-sensitive yeast, specifically Saccharomycescerevisiae, comprising disrupted or deleted genes SNQ2, PDR12, YOR1, CKA2, LCB3, and optionally any one of VPS51, VPS52, or VPS53 and its use for detecting inhibitory activity of fumonisins or detecting fumonisin detoxifying compounds in a sample. The fumonisin-sensitive yeast is also useful for screening of fumonisin detoxification or resistance conferring activity of heterologous polypeptides.

The invention relates to a fumonisins degrading enzyme with improved activity and application of the fumonisins degrading enzyme.

The present invention relates to the use of an animal feed additive that reduces the toxic effects of mycotoxins, especially fumonisin B1 and zearalenone. This additive is a mycotoxin adsorbent consisting of an organically modified phyllosilicate with 71% substitution of its cation exchange capacity with the organic compound dioctadecyldimethylammonium chloride.

The application relates to a traditional Chinese medicine and additive compound preparation for treating gizzarditis of laying hens and application, and relates to the technical field of veterinary drugs. The compound preparation is prepared by mixing a traditional Chinese medicine extract and a functional feed additive. The raw material composition of the traditional Chinese medicine extract comprises torrefied malt, torrefied medicated leaven, radix codonopsis, rhizoma atractylodis macrocephalae, rhizoma coptidis, andrographis, dried tangerine or orange peel, white arillus, and liquorice. The additive comprises neutral protease, a mould inhibitor and probiotics. The mould inhibitor is selected from aspergillus flavus B1 degrading enzyme, corn strobukenone degrading enzyme, fumonisin degrading enzyme and vomitoxin degrading enzyme. The probiotics are selected from lactobacillus plantarum, bacillus subtilis and fructo-oligosaccharide. The application is based on the traditional Chinese veterinary theory of food digestion and spleen health, dampness expelling and qi regulation, heat clearing and toxin resolving and mucosa repairing. In combination with the effects of the additive on digestion, mould expelling and intestinal micro-ecological regulation, the application has a remarkable treatment effect on gizzarditis of laying hens, can improve the feed intake and weight gain, reduce the gizzard index, promote the repair of gastric mucosa, and has no antibiotic residue and no toxic side effect.

The invention belongs to the field of crop disease resistance identification. The invention discloses a method for accurately evaluating the resistance of a corn variety to fusarium verticillium ear rot. The method comprises the following steps: inoculating corn ears by adopting a needling inoculation method, harvesting, threshing and grinding the ears in a harvesting period, measuring the content of fumonisins in grains, and evaluating the resistance of a corn variety to fusarium verticillium ear rot through the content of fumonisins in the grains. According to the method, the resistance of the corn variety to the fusarium verticillium ear rot can be evaluated more accurately, and the corn variety harmful to human and livestock is eliminated, so that the food safety is guaranteed.

The present invention belongs to the technical field of microorganism and plant disease prevention and control, and specifically, relates to a composition for preventing and controlling blueberry root and stem diseases and its application, wherein the composition comprises: a) Bacillus amyloliquefaciens, b) bamboo shoot fiber powder, corn pollen polysaccharide, cattle bone meal, mineral potassium humate and polyglutamic acid, and c) biomass power plant ash; wherein the preservation number of Bacillus amyloliquefaciens is CCTCC NO: M20221206. Applying the composition during the blueberry planting process can significantly reduce the content of fungal toxins such as fusaric acid, fumonisin and trichothecene in the rhizosphere soil of blueberries, increase the content of resistance substances such as salicylic acid, jasmonic acid and phenolic substances in the root system, and reduce the fungal toxins caused by Fusarium solani ( Fusarium solani ), Fusarium equisetum ( Fusarium horsetail ), and / or, Pythium ( Pythium sterile )、Rhizoctonia solani( Rhizoctonia solani )、Fusobacterium spp. Neofusicoccus minor ) to measure the disease index of blueberry rhizome diseases caused by infection, and ultimately achieve effective prevention and control of blueberry rhizome diseases.

The invention relates to a natural composite trace element modified mycotoxin adsorbent and a preparation method thereof, the natural composite trace element modified mycotoxin adsorbent comprises the following raw materials by weight: 80-90 parts of natural rare earth zeolite eutectic ore, 5-10 parts of composite trace elements, 3-5 parts of an active ion modifier, and 1-2 parts of a dispersant; the natural rare earth zeolite eutectic ore originates from a Bayan Obo mining area, is high-purity crystallized hydrated sodium aluminosilicate calcium, and contains a zeolite and montmorillonite eutectic structure. Through synergistic modification of the natural mineral carrier and the compound trace elements, broad-spectrum efficient adsorption of various mycotoxins such as aflatoxin, fumonisins and zearalenone is achieved, and the adsorption stability in the full pH range of a digestive system is excellent; meanwhile, the feed has the multifunctional characteristics of resisting bacteria, removing heavy metals and promoting animal growth, does not influence absorption of nutritional ingredients such as vitamins and mineral substances in the feed, is nontoxic and harmless, is not accumulated in animal bodies, is simple and controllable in preparation process and low in cost, and is suitable for the fields of livestock and poultry and aquaculture.

The invention belongs to the technical field of protein engineering modification, and particularly relates to a method for improving thermal stability of amine oxidase ACAO and a mutant. Compared with a wild type enzyme, the temperature tolerance of the mutant enzyme in a certain temperature range is superior to that of the wild type enzyme, and the thermal stability is remarkably improved. Through molecular modification on a wild type, the thermal stability of amine oxidase ACAO mutant enzyme is remarkably improved. The enzyme is mainly applied to the industries of food production, livestock feed processing and the like so as to reduce the harm of fumonisins to the health of animals and human beings. The thermal stability improved amine oxidase ACAO mutant obtained by the invention is beneficial to improving the production efficiency of continuous production of enzyme in actual industrial application, has good industrial application prospects, and lays a foundation for further research on the structure of amine oxidase.

The invention discloses lazy bacillus for degrading fumonisins, a fumonisins degrading enzyme and application of the fumonisins degrading enzyme. The invention firstly discloses lazobium sp. And application of the lazobium sp. In degradation or removal of fumonisins. The invention further discloses a fumonisins degrading enzyme or a composition thereof and application of the fumonisins degrading enzyme or the composition thereof in degradation or removal of fumonisins. According to the lazelibacter ASAG15 and the three fumonisins degrading enzymes which are excavated from the lazelibacter ASAG15 and have good fumonisins degrading or removing effects, degradation products of the fumonisins can be further degraded or removed through combined use, and the lazelibacter ASAG15 and the three fumonisins degrading enzymes can be applied to industries such as agriculture, feed and food and have wide application prospects. The method has a good application prospect in the fields of degradation, removal, prevention and control of fumonisins.

The utility model relates to the technical field of test strips, and discloses a chromatography type fumonisins test strip which comprises a main body shell, a limiting groove is formed in the side wall of an inner cavity of the main body shell, a filter is fixedly connected to the side wall of the inner cavity of the main body shell, a first filtering hole is formed in the side face of the filter, and a second filtering hole is formed in the side face of the filter. A filter block is fixedly connected to an inner cavity of the main body shell, second filter holes are formed in the side face of the filter block, a liquid retention cavity is formed in the side face of the filter block, a liquid inlet is formed in the side face of the liquid retention cavity, and a liquid outlet pipe is fixedly connected to the side face of the liquid inlet. According to the device, grains such as corn and the like can be directly ground and fully and uniformly mixed with a diluent, then the mixture is directly poured into a filter, an extrusion block is used for extruding, residues are filtered and separated, separated liquid can directly flow into a liquid outlet pipe and then is dropped into a sample adding hole, and the situation that the liquid needs to be dropped into a sample adding hole through a suction pipe after filtering is conveniently omitted.

The present invention relates to the use of an enzyme comprising an amino acid sequence having at least 80% sequence identity with sequence SEQ ID NO: 1 for degrading fumonisins in a sample. Furthermore, the present invention relates to a method of degrading fumonisins based on incubating a contaminated sample with an enzyme comprising an amino acid sequence having at least 80% sequence identity to the sequence SEQ ID NO: 1.

The invention belongs to the technical field of chemical synthesis. The invention provides a synthesis method of fumonisins series hidden toxins, and the synthesis method of the fumonisins series hidden toxins comprises the following steps: taking fumonisins as a raw material, and respectively carrying out synthesis of N-alkylated fumonisins, synthesis of hydrolyzed fumonisins and synthesis of N-amidated fumonisins. The invention also provides application of the fumonisins series hidden toxins synthesized by the synthesis method in preparation of toxin standard products. The fumonisins serve as raw materials, a series of fumonisins series hidden toxins are synthesized, the synthesis method is simple in step and efficient, and the purity of the synthesized fumonisins series hidden toxins is larger than 99%. The fumonisins series hidden toxins developed by the invention can be used for related detection, and have important significance on supervision and detection work in related food safety fields.

The invention belongs to the technical field of microorganism and plant disease prevention and treatment, and particularly relates to a composition for preventing and treating blueberry rhizome diseases and application thereof.The composition is prepared from bacillus amyloliquefaciens, bamboo shoot fiber powder, corn pollen polysaccharide, cattle bone powder, mineral source potassium fulvate and polyglutamic acid and biomass power plant ash; wherein the preservation number of the bacillus amyloliquefaciens is CCTCC (China Center For Type Culture Collection) NO: M20221206. When the composition is applied in the blueberry planting process, the content of mycotoxins such as fusaric acid, fumonisins and trichothecin in blueberry rhizosphere soil can be remarkably reduced, the content of resistant substances such as salicylic acid, jasmonic acid and phenolic substances in a root system can be increased, and the content of the resistant substances such as Fusarium solani, Fusarium equiseti and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani and / or Fusarium solani can be reduced. According to the method, the disease index of the root and stem diseases of the blueberries caused by infection of bacillus subtilis, pythium sterilum, rhizoctonia solani and Neofusicoccum parvum is calculated according to the disease index of the root and stem diseases of the blueberries, and finally, effective prevention and control of the root and stem diseases of the blueberries are achieved. The method has the advantages that the disease index of the root and stem diseases of the blueberries caused by infection of bacillus subtilis, pythium sterilum, rhizoctonia solani and Neofusicoccum parvum is calculated according to the disease index of the root and stem diseases of the blueberries;

The invention relates to the technical field of agricultural biology, in particular to a nucleic acid aptamer for detoxification of fumonisins B1 and application of the nucleic acid aptamer. The nucleotide sequence of the nucleic acid aptamer provided by the invention is shown as any one or more sequences of SEQ ID NO.1 to SEQ ID NO.3. The invention also provides a preparation method of the nucleic acid aptamer. The nucleic acid aptamer has the advantages of high efficiency, convenience in operation, no need of specific professional equipment, no influence on nutrient substances of agricultural and sideline products, no generation of harmful substances in the detoxification process and no need of multi-enzyme synergistic degradation in the detoxification aspect of the fumonisins B1, and also has the advantages of no requirement on environmental conditions and the like; and the detoxification of the fumonisins B1 can be completed at room temperature. The nucleic acid aptamer provided by the invention can overcome part of defects and limitations of the antibody, and is low in immunogenicity, easy in-vitro synthesis, simple in modification and the like. The nucleic acid aptamer can be applied to agriculture, feed and other industries, and harm of FB1 to animal and human health is reduced.

The application belongs to the technical field of protein engineering, and particularly relates to a fumonisin-degrading enzyme mutant with improved specific activity and thermal stability and application. The fumonisin-degrading enzyme FumDSB mutant A373R, A373R / S248P, A373R / Q259P, A373R / T282D, A373R / G321A, A373R / D330P and A373R / A378K of the application show significantly improved relative activity and thermal stability compared with wild-type fumonisin-degrading enzyme. The enzyme is mainly applied to the industries of agricultural products, animal feed, food and the like to reduce the health hazards of fumonisin to animals and humans. The fumonisin mutant with improved activity and thermal stability obtained by the application is conducive to reducing the cost of the enzyme in industrial application.

This invention discloses a strain of *Trichoderma viride* and its application in antibacterial and toxin degradation, belonging to the field of microbial technology. The *Trichoderma viride* strain is deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 41619. The *Trichoderma viride* strain exhibits a significant inhibitory effect on the growth and infection of *Aspergillus flavus*, with an inhibitory effect superior to 4000 times dilution of tebuconazole. This *Trichoderma viride* strain can produce enzymes that degrade the cell walls of pathogenic fungi. Fungal cell walls are composed of cellulose, glucan, chitin, and glycoproteins. The chitinase and cellulase produced by *Trichoderma viride* can degrade the fungal cell walls, thereby inhibiting the growth, development, and infection of *Aspergillus flavus*. Furthermore, this invention also experimentally demonstrates that *Trichoderma viride* has a highly efficient degradation effect on aflatoxin and fumonisin.