11 results about "Fumonisin B1" patented technology

The invention discloses bacillus subtilis, a complex microbial inoculant and application of the complex microbial inoculant to degradation of mycotoxin, and belongs to the field of microorganisms and fermentation engineering. According to the invention, a strain of bacillus subtilis BLYC-1937 with the activity of degrading mycotoxin is obtained through screening, and the bacillus subtilis BLYC-1937 has a good degradation effect on pollution of single toxin of T-2 toxin, vomitoxin, fumonisins B1, aflatoxin B1 and ochratoxin A or combined pollution of multiple toxins; the strain has good antagonism on fusarium oxysporum capable of producing T-2 toxin and aspergillus ochraceus capable of producing ochratoxin A, and pollution of T-2 toxin and ochratoxin A can be reduced from the source.

The invention relates to an aptamer specifically bound with aflatoxin B1 and application of the aptamer, and belongs to the technical field of biological detection. According to the invention, aflatoxin B1 is taken as a target, on the basis of broad-spectrum recognition of aflatoxin (aflatoxin B1, aflatoxin B2, aflatoxin M1 and aflatoxin G1) by an original aptamer, an aptamer sequence is modified by using directed evolution, and the aflatoxin B1, aflatoxin B2, aflatoxin M1 and aflatoxin G1 are identified on the basis of molecular dynamics in combination with free energy. And the aptamer sequence for recognizing the aflatoxin B1 with high affinity and specificity is obtained. The dissociation constant of the aptamer to the aflatoxin B1 is 9.2 nM, and the aptamer has high affinity to the aflatoxin B1; in addition, the aptamer provided by the invention can only detect the aflatoxin B1, has no recognition force on the aflatoxin B2, the aflatoxin M1, the aflatoxin G1, the fumonisins B1, the zearalenone and the ochratoxin A, and has good specificity.

The present invention relates to the use of an animal feed additive that reduces the toxic effects of mycotoxins, especially fumonisin B1 and zearalenone. This additive is a mycotoxin adsorbent consisting of an organically modified phyllosilicate with 71% substitution of its cation exchange capacity with the organic compound dioctadecyldimethylammonium chloride.

The present application relates to a kind of biological sensor for detecting fumonisin B1 and its preparation method and application, the biological sensor includes dlucCage protein and lucKey protein;The amino acid sequence of the dlucCage protein is as shown in SEQ ID NO:1, or as the polypeptide with one, two, or three amino acid mutations to serine on sequence SEQ ID NO:1;The amino acid sequence of the lucKey protein is as shown in SEQ ID NO:2, or as the polypeptide with one, two, or three amino acid mutations on sequence SEQ ID NO:2.The biological sensor of the present application creatively realizes the good cooperation of antigen mimic epitope and dlucCage protein allosteric function, the connecting peptide of molecular head-to-tail extension design can stably connect larger size target binding motif, effectively break through the space limitation problem of traditional lucCage protein bolt domain to target binding motif grafting, with high sensitivity, strong specificity, signal readout fast and the like advantages, has good application prospect in the field of food safety detection.

The invention relates to a biosensor for detecting fumonisin B1 as well as a preparation method and application of the biosensor. The biosensor comprises a lucCage protein and a lucKey protein, the lucCage protein is composed of a cage structural domain and a bolt-lock structural domain; the sequence of the cage structural domain is as shown in SEQ ID NO. 1; the bolt-lock structural domain is composed of a weak binding sequence 1, a split luciferase small fragment SmBit, a weak binding sequence 2 and a fumonisin B1 polypeptide mimic epitope; the lucKey protein is composed of a key peptide, a connecting peptide and a fission luciferase large fragment LgBit, wherein the amino acid sequence of the key peptide is shown as SEQ ID NO.6. According to the biosensor, the polypeptide antigen mimic epitope is creatively grafted to the lucCage, so that the biosensor has the attribute of detecting the antigen, retains the characteristics of the original lucCage allosteric protein, has the advantages of simplicity and convenience in preparation, low cost, high sensitivity, strong specificity, rapidness in signal reading and the like, and has a good application prospect in the field of food safety detection.

The invention discloses a method for synchronously and quickly detecting various mycotoxins by competitive immunochromatography based on a microbial nano-enzyme label and application, and the method can be used for simultaneously and accurately detecting various common mycotoxins, including aflatoxin B1, zearalenone and fumonisins B1, by utilizing the enhanced catalytic effect mediated by the microbial nano-enzyme label. The surface of Escherichia coli is coated with a SiO2 interlayer so as to improve the structural stability and the solution dispersity, and meanwhile, the surface of Escherichia coli is coated with a layer of catalytic shell composed of Au (at) Pt nanoparticles so as to generate excellent colorimetric and catalytic performance. According to the application, ESi-Au-Pt is introduced into a nano enzyme-ICA system, so that the sensitivity and accuracy of on-site analysis of a complex sample are remarkably improved, the limit of detection (LOD) of synchronous detection of target fungaltoxin is reduced to 6.1 / 1.6 / 5.4 pg / mL, the detection range spans 4-5 orders of magnitude, in addition, the established method shows excellent accuracy and stability in a complex matrix, and the method can be applied to the field analysis of fungaltoxin. The method shows a huge potential in the field-based real-time monitoring of small-molecule pollutants.

The invention relates to a biosensor for detecting fumonisin B1 as well as a preparation method and application of the biosensor. The biosensor comprises dlucCage protein and lucKey protein, the amino acid sequence of the dlucCage protein is as shown in SEQ ID NO: 1, or the dlucCage protein is a polypeptide with one, two or three amino acids mutated into serine on the sequence SEQ ID NO: 1; the amino acid sequence of the lucKey protein is as shown in SEQ ID NO: 2, or the lucKey protein is a polypeptide with one, two or three amino acids mutated on the sequence SEQ ID NO: 2. The biosensor creatively realizes a good synergistic effect of an antigen mimic epitope and a dlucCage protein allosteric function, the connecting peptide designed at the head and the tail of a molecule in an extending manner can be stably connected with a target binding motif with a larger size, the problem of space limitation of a bolt structural domain in a traditional lucCage protein on target binding motif grafting is effectively broken through, and the biosensor has a good application prospect. The method has the advantages of high sensitivity, strong specificity, rapid signal reading and the like, and has a good application prospect in the field of food safety detection.

The application belongs to the technical field of biosensors, and particularly relates to a preparation method of a photocatalytic signal amplification electrochemical biosensor for detection of fumonisin B1 and application thereof. The synthetic composite material of graphdiyne-methylene blue-gold nanorod is used as a base material to generate an electrochemical signal; a single-stranded DNA is self-assembled to prepare a DNA tetrahedral nanostructure, the size of the tetrahedron is adjusted by controlling the number of bases, and the probe is controllably assembled on the electrode surface; the cadmium selenide quantum dots connected to the DNA composite structure are used as a signal transduction element to realize amplification of the amount of the fumonisin B1-induced electrochemical signal change by degrading the methylene blue. The electrochemical aptamer sensor constructed in the application is used for detection of fumonisin B1, has high sensitivity and good selectivity, the detection range thereof spans five orders of magnitude, and the detection limit is as low as 0.45fg / mL ‑1 , and remarkable effects are achieved.

The invention particularly relates to a photoelectrochemical biosensor based on monatomic bismuth and a detection method of fumonisins B1. At present, the detection of the fumonisin B1 has the defects of complex pretreatment and expensive detection equipment. The photoelectrochemical detection sensor is composed of an electrode, a catalytic activity substrate material, an aptamer recognition element and a signal amplification unit, a monatomic bismuth nano-material generates a remarkable photoelectric response signal, and by means of the specific molecular recognition effect of an aptamer and a target object, the photoelectrochemical detection sensor of the fumonisins B1 can be used for detecting the fumonisins B1. The high-selectivity recognition of the fumonisin B1 is realized; meanwhile, MXenes quantum dots are introduced to serve as a signal amplification medium, and light current response is enhanced. Researches show that the integrated sensing platform has excellent performance in the aspects of detection sensitivity, analysis speed and result accuracy, and a novel analysis strategy is provided for mycotoxin detection.

The present invention relates to the field of genetic engineering, particularly to a fumonisin degrading enzyme FumDPS, gene and application thereof. The present invention provides a fumonisin degrading enzyme FumDPS from Pigmentiphaga sp. which has the amino acid sequence of SEQ ID NO: 1, has the optimum temperature of 37° C., and the optimum pH of 8 to 9, remains more than 60% of the activity in the range of pH 3 to 7, and has 100% of degradation rate of fumonisin B1 at the optimum temperature and pH. The enzyme of the present invention can be applied to reduce the harm of fumonisin to animals and human health in agriculture, feed and food.