The present application relates to a kind of biological sensor for detecting
fumonisin B1 and its preparation method and application, the biological sensor includes dlucCage
protein and lucKey
protein;The
amino acid sequence of the dlucCage
protein is as shown in SEQ ID NO:1, or as the polypeptide with one, two, or three
amino acid mutations to
serine on sequence SEQ ID NO:1;The
amino acid sequence of the lucKey protein is as shown in SEQ ID NO:2, or as the polypeptide with one, two, or three amino acid mutations on sequence SEQ ID NO:2.The biological sensor of the present application creatively realizes the good cooperation of
antigen mimic
epitope and dlucCage protein allosteric function, the connecting
peptide of molecular head-to-
tail extension design can stably connect larger size
target binding motif, effectively break through the space limitation problem of traditional lucCage protein bolt domain to
target binding motif
grafting, with high sensitivity, strong specificity,
signal readout fast and the like advantages, has good application prospect in the field of
food safety detection.