4 results about "Fumonisin B1" patented technology

The present invention relates to the use of an animal feed additive that reduces the toxic effects of mycotoxins, especially fumonisin B1 and zearalenone. This additive is a mycotoxin adsorbent consisting of an organically modified phyllosilicate with 71% substitution of its cation exchange capacity with the organic compound dioctadecyldimethylammonium chloride.

The present application relates to a kind of biological sensor for detecting fumonisin B1 and its preparation method and application, the biological sensor includes dlucCage protein and lucKey protein;The amino acid sequence of the dlucCage protein is as shown in SEQ ID NO:1, or as the polypeptide with one, two, or three amino acid mutations to serine on sequence SEQ ID NO:1;The amino acid sequence of the lucKey protein is as shown in SEQ ID NO:2, or as the polypeptide with one, two, or three amino acid mutations on sequence SEQ ID NO:2.The biological sensor of the present application creatively realizes the good cooperation of antigen mimic epitope and dlucCage protein allosteric function, the connecting peptide of molecular head-to-tail extension design can stably connect larger size target binding motif, effectively break through the space limitation problem of traditional lucCage protein bolt domain to target binding motif grafting, with high sensitivity, strong specificity, signal readout fast and the like advantages, has good application prospect in the field of food safety detection.

The application belongs to the technical field of biosensors, and particularly relates to a preparation method of a photocatalytic signal amplification electrochemical biosensor for detection of fumonisin B1 and application thereof. The synthetic composite material of graphdiyne-methylene blue-gold nanorod is used as a base material to generate an electrochemical signal; a single-stranded DNA is self-assembled to prepare a DNA tetrahedral nanostructure, the size of the tetrahedron is adjusted by controlling the number of bases, and the probe is controllably assembled on the electrode surface; the cadmium selenide quantum dots connected to the DNA composite structure are used as a signal transduction element to realize amplification of the amount of the fumonisin B1-induced electrochemical signal change by degrading the methylene blue. The electrochemical aptamer sensor constructed in the application is used for detection of fumonisin B1, has high sensitivity and good selectivity, the detection range thereof spans five orders of magnitude, and the detection limit is as low as 0.45fg / mL ‑1 , and remarkable effects are achieved.