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91 results about "Fumonisin B1" patented technology
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Fumonisin B₁ is the most prevalent member of a family of toxins, known as fumonisins, produced by several species of Fusarium molds, such as Fusarium verticillioides, which occur mainly in maize (corn), wheat and other cereals. Fumonisin B1 contamination of maize has been reported worldwide at mg/kg levels. Human exposure occurs at levels of micrograms to milligrams per day and is greatest in regions where maize products are the dietary staple.
Electrochemical sidestream immune quantitative test paper sensor based on microgap array electrode and method thereof for detecting biotoxin
InactiveCN101509924AEasy to makeLow costComponent separationMaterial analysis by electric/magnetic meansFumonisin B1Ochratoxin A
The invention relates to an electrochemical lateral-flow immunity quantitative test paper sensor based on microclearance array electrode, and a method thereof used for detecting biological toxins such as ochratoxin A and fumonisin B1. The sensor includes two parts, namely, immune chromatographic analysis test paper strip and electrochemical detecting part. The fast detecting test paper sensor has strong specificity, can realize quantitative detection, can be used at the temperature between 4 and 40 DEG C, and the result can be observed after ten minutes, thus being suitable for units or individuals to quickly detect ochratoxin A and fumonisin B1 in animal derived food samples, and being expected to become an effective technical means for the field screening of ochratoxin A and fumonisin B1 in food and feed samples.
Owner:湖南省宜生科技有限公司 +1
Screening and application of single-chain antibody against fumonisin
InactiveCN102453093AHigh affinityReduce manufacturing costImmunoglobulins against fungi/algae/lichensBiological testingEscherichia coliSingle-Chain Antibodies
The invention belongs to the technical field of genetic engineering, and in particular relates to screening of a single-chain antibody against fumonisin and application thereof in immunoassay of fumonisin. The screening method comprises the following steps of: directly setting off from coupled antigen FB1-KLH(keyhole limpet hemocyanin) immunized mouse spleen cells, establishing a single-chain antibody gene library by a molecular cloning method and technology, then screening by a phage display technology, expressing ELISA(enzyme-linked immuno sorbent assay) test, sequencing, and finally obtaining the single-chain antibody against fumonisin named as FB-Mu 1H3 with high affinity and a coding gene thereof. The single-chain antibody can be directly applied to assay of fumonisin after being expressed and purified in a large quantity in escherichia coli, and the applications include assay of fumonisin pollution in field crops, feed, grain or food.
Owner:HUAZHONG AGRI UNIV
Method for detecting fumonisin B1 based on fluorescence resonance energy transfer
ActiveCN103901000AImprove accuracyImprove stabilityFluorescence/phosphorescenceEnergy transfer upconversionMagnetite Nanoparticles
The present invention provides a method for detecting fumonisin B1 based on fluorescence resonance energy transfer. The method comprises that: NaYF4:Yb,Ho upconversion fluorescence nanoparticles and gold nanoparticles are respectively and covalently bound to both ends of the stem-like part of the specific molecular beacon, wherein the fluorescence resonance energy transfer upconversion fluorescence can be quenched by the gold nanoparticles; and a fumonisin B1 aptamer and a complementary strand thereof are subjected to hybridization immobilization on the surface of magnetic nanoparticles, an analyte fumonisin B1 is added and is specifically bound with the aptamer so as to make the complementary strand be separated from the magnetic nanoparticles, and the separated complementary strand and the molecular beacon are subjected to hybridization so as to open the ring-like part of the molecular beacon, such that the fluorescence resonance energy transfer system is broken, the fluorescence signal is restored, and the fumonisin B1 content can be determined by detecting the fluorescence signal intensity, wherein the detection limitation of the method achieves 0.01 ng.mL<-1>. According to the present invention, the fumonisin B1 content in the food sample can be rapidly, accurately e and sensitively detected.
Owner:JIANGNAN UNIV
Kit and method for quantitative determination of Fumonisin B1 content in food by ELISA detection technique
The invention relates to a reagent box for quantificationally detecting the content of fumonisin B1 in food by adopting an enzymoimmunoassay technology and the detecting method thereof. The reagent box includes the steps that a fumonisin B1-KLH antigen is prepared; a polyclonal fumonisin B1 antibody is prepared; an enzyme labeling antigen is prepared; a coated plate is prepared, and a reagent is prepared. The invention mainly adopts the enzymoimmunoassay technology to detect the fumonisin B1, can realize the aim of quantificationally detecting the content of fumonisin B1 in food and alimentary crops; and the reagent box has simple structure, convenient usage, cheap price and high sensitivity; the detecting method has strong specificity, high sensitivity, good veracity, and the sensitivity can reach gamma / kg grade.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Immunoadsorbent and composite affinity column for purification of fumonisin B1, aflatoxin, ochratoxin A and zearalenone
ActiveCN106977600AImprove performanceFast detection methodComponent separationOther chemical processesFumonisin B1Immunosorbents
The present invention relates to an immunoadsorbent and a composite affinity column for purification of fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The immunoadsorbent comprises a solid carrier and fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies coupled with the solid carrier, the fumonisin B1 monoclonal antibody is secreted from hybridoma cell line Fm7A11 with the preservation number of CCTCCNO.C201636, the hybridoma cell line Fm7A11 is preserved in CCTCC in March 29, 2016 in Wuhan University Wuhan China. The composite affinity column is loaded with the immunoadsorbent with the fumonisin B1, aflatoxin, ochratoxin A and zearalenone monoclonal antibodies, and is used for purification of the fumonisin B1, aflatoxin, ochratoxin A and zearalenone. The composite affinity column can be used for purification and detection of fumonisin B1, aflatoxin, ochratoxin A and zearalenone toxin samples.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Preparing method for magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1
InactiveCN105717285AQuick identificationRapid enrichmentBiological testingFluorescence/phosphorescenceMagnetic beadFumonisin B1
The invention provides a preparing method for a magnetic control ratio fluorescence adapter sensor for sensitivity detection of fumonisins B1. The method comprises the following steps that a CdTe quantum dot aqueous solution emitting green fluorescence and a CdTe quantum dot aqueous solution emitting red fluorescence are prepared; a SiO2 nanosphere dispersing solution is prepared; a Fe3O4@SiO2 magnetic bead dispersing solution is prepared; a gQDs-covered SiO2 dispersing solution is prepared; an rQDs-covered mSiO2 nanosphere dispersing solution is prepared; a SiO2@gQDs-marked Aptamer fluorescence probe dispersing solution is prepared; an mSiO2@rQDs-marked cDBA magnetic-fluorescence probe dispersing solution is prepared; the sensor is structured, and a sample is detected; the sample to be detected is detected. The magnetic control ratio fluorescence adapter sensor developed through the preparing method is simple in processing method, convenient to use, low in detection cost, and capable of providing internal correction to eliminate interferences of factors such as environment factors, stimulating strength changes and probe concentration, greatly reducing errors, and improving accuracy and sensitivity of FB1 detection.
Owner:JIANGSU UNIV
Time-resolved fluoroimmunoassay kit for detecting fumonisins B1 and detection method thereof
InactiveCN101699293ASimple structureEasy to useFluorescence/phosphorescenceTime resolved fluorescence immunoassayFumonisin B1
The invention discloses a time-resolved fluorescence immunoassay kit for detecting fumonisins B1 and a detection method thereof, belonging to the technical field of time-resolved fluoroimmunoassay (TRFIA) for detecting the content of FB1 in grain, fodder and food. The prepared kit adopts the TRFIA to detect the FB1. A micropore plate is coated with FB1-BSA, is added with FB1 standard or sample, and is added with FB1 monoclonal antibody. Dissociative FB1 and the FB1-BSA on the micropore plate compete for the FB1 monoclonal antibody, unconnected FB1 monoclonal antibody is removed by means of washing, Eu3+ -sheep anti-mouse antibody is added, and the unconnected Eu3+ -sheep anti-mouse antibody is removed by means of washing after labeled immune reaction. After adding strengthening liquid, fluorescence intensity (cps) is detected by a time-resolved fluoroimmunoassay apparatus, wherein the fluorescence intensity is in inverse proportion to the concentration of the FB1, and the content in the FB1 can be ensured by contrasting a standard curve. The kit has simple structure, convenient use, low price and high sensitivity more than 0.05ng / mL.
Owner:JIANGSU INST OF NUCLEAR MEDICINE
Oligonucleotides aptamer special for distinguishing fumonisin B1
ActiveCN103013999AHigh purityImprove efficiencyOrganic compound preparationMicrobiological testing/measurementChemical synthesisMagnetic bead
The invention provides a group of single-stranded DNA nucleic acid aptamers of fumonisin B1. Each aptamer is obtained by carrying out screening, amplification, sequencing, and analysis on affinity and specificity on a random original single-stranded DNA library with the combination of an SELEX (systematic evolution of ligands by exponential enrichment) technology and a magnetic bead with fumonisin B fixed on the surface. As a special efficient distinguishing aptamer of fumonisin B, the group of DNA aptamers provides a new choice for developing a method for replacing the existing methods which are used for detecting fumonisin B by depending on an antibody, and can be used for analyzing and detecting or separating the fumonisin B in the environment of enriched food. The nucleic acid aptamer is small in molecular weight, low in chemical synthesis cost, easy to mark, strong in affinity and specificity, reversible in degeneration renaturation, high in speed, and suitable for repeated use, room-temperature transportation and long-term storage.
Owner:JIANGNAN UNIV
Method for degrading fumonisins
The invention discloses a method for degrading fumonisins, which uses electron beams to radiate a sample containing the fumonisins to obtain the sample with reduced fumonisins content. The method can directly radiate finished products such as agricultural products, food, and feed, has simple operation and obvious effects, and makes the degradation rate of fumonisins B1 reach 59.03 percent.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Anti-aflatoxin general type monoclonal antibody hybridoma cell line and application thereof
ActiveCN104004717AHigh detection sensitivityHigh affinityMicroorganism based processesTissue cultureBALB/cPolyethylene glycol
Owner:无锡迪腾敏生物科技有限公司 +1
Preparation method of electrochemical adapter sensor for detecting fumonisin B1 ( FB1)
InactiveCN106706733AAchieving Sensitive DetectionMultiple load spacesMaterial nanotechnologyMaterial electrochemical variablesInstrumentationChemistry
The invention provides a preparation method of an electrochemical adapter sensor for detecting fumonisin B1 (FB1). The preparation method comprises the following steps of step one, preparation of an silk-printed carbon electrode (SPCE); step two, preparation of an activated FB1 aptamer solution; step three, preparation of an Au nanoparticles (AuNPs) modified SPCE; step four, preparation of an FB1 aptamer modified AuNPs-SPCE; step five, sealing of spare active sites on the surface of the electrode. According to the preparation method of the electrochemical adapter sensor for detecting the FB1, provided by the invention, the AuNPs are adopted for modifying the surface of the electrode, on one hand, the large amount of AuNPs are loaded on the surface of the electrode, so that a signal amplification function on label-free impedance method detection is obtained; on the other hand, more sulfhydrylated adapters are fixed to the surface of the electrode by utilizing Au-S bonds. An FB1 impedance detection method provided by the invention has the characteristics that the operation is more simple, convenient and flexible, instruments and equipment are simpler, the dosage of a reagent is less, the detection cost is low, and the like.
Owner:JIANGSU UNIV
Quantitative detection method of fumonisins B1
InactiveCN102520179AFast processDetection object is singleMaterial analysisFumonisin B1Monoclonal antibody
The invention relates to a quantitative detection method of fumonisins B1, comprising the following steps of: 1, preparing conjugates FB1-KLH and FB1-OVA; 2, preparing anti-FB1 monoclonal antibody; 3, preparing colloid gold, labeling the anti-FB1 monoclonal antibody and spraying the labeled monoclonal antibody onto a gold labeled gasket; 4, spotting on a nitrocellulose membrane; 5, assembling to form strips; and 6, drawing a standard curve of FB1 concentration and color value and dragging the color value of a sample to be tested into the standard curve to obtain the FB1 content in the sample to be tested. The invention has advantages of single detection object and strong pertinence, high accuracy, fast detection speed and short required time. The method provided by the invention can be used for detection without trained professionals. The method satisfies the demand of rapid and correct judgment of FB1 content in food storage and sale institution, exit-entry and custom inspection departments, and is convenient for popularization and application at grass-root level.
Owner:SHANGHAI JIAO TONG UNIV
Method for simultaneously detecting zearalenone and fumonisin B1
ActiveCN110806439AIncrease transfer rateEasy to fixMaterial electrochemical variablesAptamerFerrocenyl group
The invention discloses a method for simultaneously detecting zearalenone (ZEN) and fumonisin B1 (FB1), and also discloses an aptamer sensor for simultaneously detecting the zearalenone and the fumonisin. The aptamer sensor is prepared by the following method: (1) covering a glassy carbon electrode with reducing molybdenum disulfide and a gold nanoparticle composite (rMoS2-Au) nanomaterial, and drying; (2) subsequently dripping a mixed solution of a zearalenone nucleic acid aptamer (AP1) and a fumonisin B1 nucleic acid aptamer (AP2) onto the electrode, incubating for 1-10h at 1-10 mu mol / L, v / v, 1 / 1, and blocking the same with 1 mg / mL QBSA; and (3) finally, dripping a mixed solution of a self-developed ZEN nucleic acid aptamer partial complementary sequence (CP1-Au-Thi) solution of 5'-endmodified gold nanoparticles and thionine and a FB1 nucleic acid aptamer partial complementary sequence (CP2-Au-FC6S) solution of 5'-end modified gold nanoparticles and ferrocenyl hexanethiol, and incubating for 1-10h at 1-10 mu mol / L, v / v, 1 / 1 to obtain the required aptamer sensor. The method provided by the invention is suitable for the rapid and simultaneous detection of QEN and FB1 in agricultural products such as corn and feed, thereby effectively preventing the agricultural products with over-standard QEN and FB1 from flowing into the market, and the diet safety of people is guaranteed asa result.
Owner:SHANGHAI ACAD OF AGRI SCI
Immunoadsorbent for purifying five kinds of mycotoxins including fumonisin b1 and aflatoxin b1, and complex affinity column
ActiveUS20180259526A1Improve performanceFast and accurate and safeComponent separationOther chemical processesImmunosorbentsFumonisin B1
The invention relates to an immunoadsorbent for purifying five kinds of mycotoxins including fumonisin B1 and aflatoxin B1, and a complex affinity column. The immunoadsorbent comprises a solid-phase support, and an anti-fumonisin B1 monoclonal antibody, an anti-aflatoxin B1 monoclonal antibody, an anti-ochratoxin A monoclonal antibody, an anti-zearalenone monoclonal antibody and an anti-sterigmatocystin monoclonal antibody which are coupled to the solid-phase support, wherein the anti-fumonisin B1 monoclonal antibody is secreted by a hybridoma cell strain Fm7A11, and the hybridoma cell strain Fm7A11 has been preserved at China Center for Type Culture Collection, Wuhan University, Wuhan, China on Mar. 29, 2016 with the preservation number of CCTCC No. C201636. The complex affinity column can be used for purification and detection of a fumonisin B1 sample, an aflatoxin B1 sample, an ochratoxin A sample, a zearalenone sample and a sterigmatocystin sample at the same time.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Preparation method of golden flower nanoparticle immune chromatography test strip capable of simultaneously detecting deoxynivalenol and fumonisins B1 in grains
InactiveCN104569405ANo apparent cross-reactivityHigh sensitivityMaterial analysisNanoparticleFumonisin B1
The invention discloses a preparation method of a golden flower nanoparticle immune chromatography test strip capable of simultaneously detecting deoxynivalenol and fumonisins B1 in grains. The method comprises the following steps: (1) preparing a DON monoclonal antibody-golden flower nanoparticle marker and a FB1-monoclonal antibody-golden flower nanoparticle marker; (2) treating a gold-marked conjugate release pad; (3) enveloping a nitrocellulose membrane; and (4) preparing the test strip. The immune chromatography test strip is high in specificity and sensitivity, visual and intuitive in detection result, simple, convenient and fast to operate, and easy to popularize and apply within a large range, and is widely suitable for the requirements such as grain purchase site inspection, food safety inspection and customs quarantine control.
Owner:NANCHANG UNIV
FB1(fumonisin B1) matrix standard substance and preparation method thereof
InactiveCN107478478AGuaranteed monitoringAvoid inaccurate test resultsPreparing sample for investigationFreeze-dryingFumonisin B1
The invention provides a preparation method of an FB1(fumonisin B1) matrix standard substance. The method comprises steps as follows: toxigenic fungus strains of FB1 are inoculated to a culture medium of edible agricultural products and cultured for toxin production, and the highly toxigenic culture medium is lyophilized by a lyophilizer, smashed and screened; blank edible agricultural product powder is added according to the principle of geometric-proportion dilution, FB1 is enabled to reach the quantity level, the materials are mixed uniformly, and the edible agricultural product matrix standard substance of FB1 is obtained. According to the prepared edible agricultural product matrix standard substance of FB1, a value traceability substance is provided for daily FB1 residue detection, the problem of inaccurate detection results caused by matrix difference is solved, and monitoring for FB1 in the agricultural products such as corn, wheat, rice and the like is accurately guaranteed.
Owner:SHANGHAI ACAD OF AGRI SCI
Fumonisins B1 aptamer and applications thereof
InactiveCN102517291ASmall molecular weightLow costOrganic compound preparationMicrobiological testing/measurementAptamerFumonisin B1
The invention relates to an aptamer combined with high specificity and high affinity of Fumonisins B1 and applications thereof. The aptamer can be used for detection and analysis and separation and concentration of the Fumonisins B1, and has the characteristics of high specificity, high affinity, uniform quality, high stability, short development cycle, low production cost and convenience in use. The invention also relates to sequences derived from aptamer sequences, which comprise modified sequences.
Owner:CHINA NAT ACAD NANOTECH & ENG
Immunochromatography time resolution fluorescence kit for synchronization detection of mixed pollution of fumonisin B1 and other four fungaltoxin and application thereof
The present invention relates to an immunochromatography time resolution fluorescence kit for synchronization detection of mixed pollution of fumonisin B1 and other four fungaltoxin and application thereof. The immunochromatography time resolution fluorescence kit comprises an immunochromatography time resolution fluorescence test paper strip and a sample reaction bottle containing europium-labeled freeze-dried products of all monoclonal antibodies; the immunochromatography time resolution fluorescence test paper strip includes a PVC substrate, a water absorbent pad, a testing pad and a sample pad which are respectively pasted on one side of the PVC substrate from top to bottom, the testing pad is provided with a nitrocellulose membrane as a base pad, the nitrocellulose membrane is provided with a horizontal quality control line and four detection lines from top to bottom, the four detection lines are respectively coated with bovine serum albumin conjugates of all the fungaltoxin, and a fumonisin B1 monoclonal antibody is secreted by hybridoma cell line Fm7A11 with preservation number of CCTCCNO.C201636. The immunochromatography time resolution fluorescence kit can be used for synchronization detection of content of aflatoxin B1, ochratoxin A, zearalenone and the fumonisin B1, and has the characteristics of simple operation, rapidness and high sensitivity.
Owner:INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Detection kit for multiple fungaltoxins, and preparation method, detection method and application of detection kit
PendingCN113092766AAvoid interferenceHigh sensitivityBiological material analysisBiological testingAntigenFumonisin B1
The invention relates to the technical field of analysis and detection, and in particular, relates to a detection kit for multiple fungaltoxins, and a preparation method, a detection method and application of the detection kit. The kit comprises up-conversion nanoparticles respectively coupled with antigens of fungaltoxins to be detected, magnetic nanoparticles respectively coupled with antibodies of the fungaltoxins to be detected, and a standard substance solution of the fungaltoxins, and the fungaltoxins to be detected at least comprise zearalenone and fumonisin B1. The kit and the method have the advantages of high sensitivity, good specificity and the like, have important practical significance on ultra-sensitive detection of multiple fungaltoxins, and have good guiding significance on realization of a field rapid and ultra-sensitive detection technology.
Owner:INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
Method for detecting 8 kinds of mycotoxins of fructus citri medicae
InactiveCN108760929AHigh sensitivityImprove detection limitComponent separationFungal toxinOchratoxin A
The invention discloses a method for measuring 8 kinds of mycotoxins of fructus citri medicae. According to the method, the 8 kinds of mycotoxins (ochratoxin A, zearalenone, fumonisin B1, sterigmatocystin, aflatoxin B1, aflatoxin B2, aflatoxin G1 and aflatoxin G2) are measured simultaneously in a system, a Thermo Hypersil GOLD chromatographic column is adopted for separation, and 0.1% formic acidwater-formic acid acetonitrile is used as a mobile phase; an electrospray ionization mode is adopted, and negative and positive ions scan simultaneously. A result shows that according to the method, the relations of the 8 kinds of mycotoxins in the linear range are good; the detection limit is at 0.16-5 ng / mL, the recovery rate at the low, medium and high adding levels is 79.0-112.8%, and an isotope internal standard method is adopted. According to the method, the matrix effect has little influence on the mycotoxin detection result, the accuracy is high, the precision degree is high, the specificity is high, and the method is simple, convenient and fast and can be used for multi-component measurement of mycotoxins in traditional Chinese medicine materials.
Owner:广东省药品检验所
Method for simultaneously preparing standards of fumonisins B1, B2 and B3
ActiveCN104931308AThe preparation method is efficient and fastHigh purityPreparing sample for investigationFumonisin B1Fumonisin
The invention provides a method for simultaneously preparing standards of fumonisins B1, B2 and B3. The method comprises the following steps of sample extraction, rough cleaning and medium-pressure preparation. The method for simultaneously preparing the standards of the fumonisins B1, B2 and B3, provided by the invention, is stable and mature, can be suitable for preparing the fumonisins in various cereal matrixes, such as maize, rice, wheat and PDA, and has the advantages that the cost is low, the preparation flow is efficient and rapid, the purity of the standards is high, and the effect is good.
Owner:SHANGHAI ACAD OF AGRI SCI
Fumonisin degrading enzyme FumDXA as well as gene and application thereof
The invention belongs to the technical field of agricultural biology, and particularly relates to Fumonisin degrading enzyme FumDXA as well as a gene and an application thereof. The Fumonisin degrading enzyme protein FumDXA derived from Xenophilus azovorans is provided, the amino acid sequence of the Fumonisin degrading enzyme protein FumDXA is represented as SEQ ID NO.1, and the encoding gene forencoding the Fumonisin degrading enzyme is also provided. The Fumonisin degrading enzyme FumDXA has activity of degrading Fumonisin B1 and can be applied to agriculture, feed and food industries, sothat harm of Fumonisin to health of animals and humans is reduced.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Reagent kit for detecting Fumonisin B1 of genetically engineered single-chain antibody and method thereof
InactiveCN102095850AEasy to prepareEasy to useMaterial analysisEscherichia coliSingle-Chain Antibodies
The invention provides a reagent kit for detecting Fumonisin B1 of genetically engineered single-chain antibody and method thereof; in the reagent kit of the invention, the reagent comprises: sample extracting solution, a standard reagent, an enzyme labeled antigen reagent, washing solution, a BSA (bovine serum albumin) regent, a chromogenic substrate and stopping solution; the content of FB1 toxins in the sample is calculated by the following steps of sample processing, regent balancing, washing, adding sampling, washing, color developing and stopping. The Fumonisin B1 single-chain antibody adopted by the reagent kit provided by the invention can be effectively expressed in colon bacillus, and can be produced in a large scale; the producing process is simple, convenient and easy; the time, labor and money are saved. Using the reagent kit in the invention for detecting the Fumonisin B1, whether the sample is polluted by the Fumonisin B1 or not can be determined, and the content of the Fumonisin B1 is calculated in 1.5-2h. The reagent kit is convenient and fast in use, and low in cost.
Owner:FUJIAN AGRI & FORESTRY UNIV
Method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines
InactiveCN102590364AEasy and fast handlingOptimizing Chromatographic ConditionsComponent separationFiltration membraneFluorescence
The invention relates to a method for simultaneously detecting fumonisins B1 and B2 in different matrix traditional Chinese medicines, namely an on-line derivation high-performance liquid phase fluorescent detection method. The method comprises the steps of extracting, purifying and detecting a sample and particularly comprises the following steps of: adding sodium chloride in the sample; performing oscillating extraction by using a horizontal table according to the volume ratio of methanol to water of 4:1; filtering; diluting a certain amount of filtrate by adding water; filtering by using a microfiber filtration membrane; purifying by using an immunoaffinity column; drying by using nitrogen; dissolving by using methanol; and sampling. A derivation agent is prepared by the following steps of: dissolving a proper amount of o-phthaldialdehyde by using methanol; adding into a proper amount of sodium tetraborate solution; adding 2-mercaptoethanol; mixing uniformly; and passing through a microfiltration membrane. The measurement method of the sample comprises the following steps of: separating by using C18 reversed phase chromatographic column; performing gradient elution by using methanol and sodium dihydrogen phosphate as mobile phase; reacting with the derivation agent; detecting by using a fluorescence detector; and confirming the positive sample by using high-performance liquid chromatography-mass spectrum.
Owner:INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
Rice zinc finger protein gene for negative regulating plant apoptosis and promoting growth and differentiation of transgene callus
InactiveCN1451749APromote growth and differentiationPlant peptidesFermentationBiotechnologyGenetically modified rice
A zinc finger protein gene OsLSD1 of paddy rice contains 3 zinc finger structure domains and can code a protein containing 143 amino acids. Its expression is fully suppressed by rice blast bacteria. Said OsLSD1 protein is a negative regulatory factor of programmed cell death and can promote the growth differentiation of transgenic celli of rice. The transgenic tobacco expressing OsLSD1 has high tolerance to cancerogenic microbial toxin fumonisin B1 (FB1).
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Immunoassay kit and special antibody for detecting fumonisins
InactiveCN101955542AProduction economyEasy to produceFungiBacteriaSingle-Chain AntibodiesHeavy chain
The invention discloses an immunoassay kit and a special antibody for detecting fumonisins. A single-chain antibody is prepared by sequentially connecting a heavy chain variable region, short peptide connecting the heavy chain variable region and a light chain variable region, and the light chain variable region, wherein the amino acid sequence of the heavy chain variable region is represented by 1st to 118th amino acid residues of a sequence 1 from an N end; and the amino acid sequence of the light chain variable region is represented by 134th to 246th amino acid residues of the sequence 1 from the N end. The affinity constant of the antibody is 1.73*10<9>L / mol, and the half abatement (IC50) is 0.15 ng / mL. In the invention, antibody sources with high valence and high specificity are provided for establishing a method for detecting fumonisins residues in food. The kit has the advantages of high sensitivity, high accuracy, high precision, high specificity for fumonisins B1 and low cost. Therefore, the antibody, the kit and the detection method play an important role in detecting the fumonisins B1.
Owner:北京维德维康生物技术有限公司
Method for simultaneous quantitative detection of zearalenone and fumonisin B1
The invention relates to a method for simultaneous quantitative detection of zearalenone and fumonisin B1. The method comprises the steps of: 1. preparation of conjugates ZEN-BSA and ZEN-OVA, FB1-KLH and FB1-OVA; 2. preparing an anti-ZEN monoclonal antibody and an anti-FB1monoclonal antibody; 3. preparing colloidal gold, labelling the monoclonal antibodies, and spraying the labelled monoclonal antibodies to a gold labeled pad; 4. conducting sample application on a cellulose nitrate membrane; 5. carrying out assembling to obtain a test strip; and 6. respectively drawing standard curves of the concentrations and color values of ZEN and FB1, bringing the color value of a sample to be tested into the standard curves so as to obtain the content of ZEN and FB1 in the sample to be tested. The method of the invention has the advantages of high detection accuracy, fast detection speed, and short detection time, and can be used without training, thus meeting the demands for rapid and correct judging of ZEN and FB1 content of grain storage and sales organizations, as well as immigration, customs and other inspection departments.
Owner:SHANGHAI JIAO TONG UNIV
Pharmaceutical agents for treating HCV infections
InactiveUS8183005B1Decreasing replicon RNAReduce expressionOrganic active ingredientsSsRNA viruses positive-senseMicroorganismMyriocin
The present inventors examined the inhibitory activity against HCV replicon of myriocin, fumonisin B1, and ceramide trafficking inhibitor HPA-12, which are compounds derived from microorganisms such as those of the genus Aureobasidium, and found that these compounds have the effect of inhibiting the replication of HCV replicon RNAs or inhibiting the expression of HCV proteins. The present inventors further performed serine palmitoyltransferase knockdown experiments using siRNAs, and results showed that HCV replicon activity and HCV protein expression were significantly inhibited in cells with suppressed LCB1 expression. Thus, sphingolipid biosynthesis was found to be involved in HCV infection. This showed that HCV infection can be treated or prevented by inhibiting enzyme activities involved in the process of sphingolipid biosynthesis by adding compounds or knocking down a gene.
Owner:CHUGAI PHARMA CO LTD
Sensitivity detection method for fumonisin B1
ActiveCN106645686AHigh luminous intensityPrevent coagulationBiological material analysisBiological testingAntigenLuminous intensity
The invention provides a sensitivity detection method for fumonisin B1. The method includes: utilizing fluorescent microspheres embedded with quantum dots to replace conventional enzyme carriers to be coupled with fumonisin B1; using a coupling product as a competition antigen to execute direct competition ELISA (enzyme-linked immune sorbent assay). In a technical path, proper quantum dots are selected according to luminescent characteristics, a quantum dot embedding method based on fluorescent microsphere technology is further designed, and on this basis, quantum dot fluorescent microspheres are subjected to BSA embedding and then coupled with fumonisin B1 to obtain the competition antigen with better performance. In the technical scheme, a lot of quantum dots are embedded in the fluorescent microspheres through a high polymer carrier, so that the fluorescent microspheres have higher illumination intensity, and detection sensitivity can be improved effectively; the fluorescent microspheres have large grain size, so that over-high affinity between the competition antigen and an embedding antibody can be lowered to a certain degree, and detection sensitivity is improved.
Owner:NANCHANG UNIV
Composite nano material modified screen print electrode and method for detecting Fumonisin B1
ActiveCN102539499AFast processDetection object is singleMaterial electrochemical variablesFumonisin B1Colloid
The invention discloses a composite nano material modified screen print electrode and a method for detecting Fumonisin B1. A working electrode of the screen print electrode is coated with a multi-walled carbon nano tube-colloid gold-chitosan mixture film. The preparation process includes the following steps; activating the multi-walled carbon nano tube; preparing the colloid gold; uniformly mixing the multi-walled carbon nano tube and the colloid gold through the chitosan; coating the mixture on the surface of the working electrode; and completing the preparation after the working electrode is dried at 37 DEG C and the film is formed. The method for detecting Fumonisin B1 provided by the invention is realized in the way that if FB1 exists in a to-be-detected sample, the FB1 competes with FB1 antibodies in the combination with FB1-OVA, so that the antibody amount combined with the FB1-OVA on the electrode is reduced, the amount of HRP-goat-anti-mouse second antibodies on the electrode is reduced, and the current variation value delta I is influenced; and then the content of FB1 in the to-be-detected sample of grains can be judged as per the standard curve drawn through an FB1 standard product. According to the invention, the detection object is single, the pertinence is strong, the accuracy is high, and the sensitivity is strong.
Owner:SHANGHAI JIAO TONG UNIV
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