Immunochip test method of staphylococcus enterotoxins and fumonisin

A staphylococcal intestinal and immune chip technology, applied in measurement devices, instruments, fluorescence/phosphorescence, etc., can solve the problems of long detection time and cumbersome steps, and achieve the effects of saving experimental costs, wide application range and high stability
CN102455356AInactive Publication Date: 2012-05-16INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF HYGIENE & ENVIRONMENTAL MEDICINE PLA ACAD OF MILITARY MEDICAL
Publication Date
2012-05-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an immunochip test method of staphylococcus enterotoxins (SEA and SEB) and fumonisin (AFB1), so as to realize the simultaneous test on the same chip. According to the invention, for the test of AFB1, the monoclonal antibody of AFB1 is immobilized on the surface of a piece of aldehyde glass and then added to the chip together with a complete antigen labeled by a certain amount of Cy3, and the technical research on the immunochip can be carried out by using a competition method. For the test of SEA and SEB, a double-antibody sandwich method is adopted and the optimization of experiment conditions is carried out; in the test, SEA and SEB are added to a reaction tank, SE which is not combined is washed off after reaction, and then a corresponding labeled antibody is added so as to form an immobilized antibody- objected to be determinand-labeled antibody sandwich structure, and the intensity of a fluorescence signal is increased with increase of the concentration of the objected to be determinand. The method disclosed by the invention is simple in operation, low in cost, sensitive and rapid, the result can be stably and reliably obtained, and the whole test process needs 1-2 hours. A new method for rapid test of various biotoxins is provided.
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Description

technical field

[0001] The invention relates to an immune chip detection method for staphylococcus enterotoxin and fumonisin in food. Background technique

[0002] Food safety has become the focus of global attention, and there are severe and prominent problems in our country. Staphylococcal enterotoxin is an important biological toxin that causes food poisoning, and it is also a commonly used biological warfare agent; the contamination of corn and its products by fumonisins may be related to the high incidence of human esophageal cancer. Mycotoxin of major hygiene importance. Currently, there is no simple and reliable way to detect it. Commonly used biotoxin detection methods include HPLC, GC-MS, ELISA and colloidal gold immunochromatography. Because HPLC and GC-MS are laboratory confirmation methods, they have the advantages of accurate quantitative detection, good repeatability, and high sensitivity, but the operation is complicated and requires professional and techni...

Claims

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