Parischnogaster spp vespo bee venom polypeptide effective part and preparation and medical application thereof

A technology of effective parts and bee venom, applied in the field of medicine, can solve the problems of low level of industrial production support and failure to form large-scale production capacity, etc., and achieve the effect of prolonging breathing time

Inactive Publication Date: 2014-03-19
DALI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with developed countries, there is still a relatively obvious gap in the research and development of my country's TTS system, especially the low level of supporting industrial production, which has not yet formed a large-scale production capacity

Method used

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  • Parischnogaster spp vespo bee venom polypeptide effective part and preparation and medical application thereof
  • Parischnogaster spp vespo bee venom polypeptide effective part and preparation and medical application thereof
  • Parischnogaster spp vespo bee venom polypeptide effective part and preparation and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 : Preparation of active parts of vespa melanogaster bee venom polypeptide and film-coating agent

[0035] 1.1 Collection of crude venom from Vespa black-sidera:

[0036] Vespa nigricans was collected from Menghai County, Yunnan Province. The bee body specimen was identified as Parischnogaster nigricans Cameron (Vaspa nigricans) by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 11V , output automatic intermittent pulsating voltage; method of collecting wasp venom: place the venom collector in front of the hive, and collect venom from the black-side Vespa colony worker bees according to the routine poison c...

Embodiment 2

[0041] Example 2 : Preparation of active parts of Vespa melanogaster bee venom polypeptide and hydrogel

[0042] 2.1 Collection of crude venom from Vespa melanogaster:

[0043] The wasp was collected from Hekou County, Wenshan, Yunnan Province. According to Professor Guo Yunjiao, National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province, the bee specimen was identified as Parischnogaster nigrifrons Saussure. Wasp venom collection device: use the auxiliary cover type bee venom collector modified by the engineering research center, wooden frame, 80 cm long, 50 cm wide; embedded with 4 pieces of 20 cm long / 33 cm wide poisonous glass; input voltage 6V , output automatic intermittent pulsating voltage; collection method of wasp venom: put the unpainted venom collector in front of the hive, and collect venom from the staff bees of the vespa melanogaster colony in accordance with the routine poison collec...

Embodiment 3

[0048] Example 3 : Preparation of poultice from the active part of Vespa militaris venom polypeptide

[0049] 3.1 Collection of crude venom from Vespa pycense:

[0050] The Vespa palpebrae was collected from Mengla, Yunnan Province. The bee body specimen was identified as Parischnogaster mellyi Saussure by Professor Yang Zizhong of the National and Local Joint Engineering Research Center for the Development of Medicinal Special Insects, Dali University, Yunnan Province. The basic device for poison collection uses a sub-cap type bee venom collector modified by the engineering research center, with a wooden frame, 80 cm long and 50 cm wide; 4 pieces of poisonous glass with a length of 20 cm / width of 33 cm are embedded; the input voltage is 9V, Output automatic intermittent pulsating voltage; The wooden frame is painted red with red paint in advance; The wooden frame is pre-smeared with 20 milliliters of the honeycomb and honeycomb extract volatile solution (prepared Method: G...

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Abstract

The invention relates to a Parischnogaster spp vespo bee venom polypeptide effective part and preparation and medical application thereof. Specifically, the effective part is prepared by dialyzing in the Parischnogaster spp rough bee venom with a dialysis membrane with molecular weight cutoff of 25KDa, and drying the eluate by means of freeze drying or concentration under reduced pressure. By a series of pharmacodynamic experiments, the inventor verifies that the biotoxin polypeptide effective part and transdermal absorption preparation thereof have the effects of remarkably preventing and treating generation of cerebral thrombus, recovering capacity for action of animal with cerebral palsy and relieving ischemic brain injury, have stronger pharmacodynamic performance than the first-line medicines, such as plavix and Xingnaojing, can be expectedly used for preparing cardiovascular and cerebrovascular disease prevention and treatment biochemical drugs for preventing and treating ischemic cardiovascular and cerebrovascular diseases, such as myocardial infarction, cardiovascular thrombosis, cerebral thrombosis, cerebral infarction and acute / chronic cerebral apoplexy, and sequelae caused by cerebral infarction injury, such as hemiplegia, hemidysesthesia, hemianopsia, facial-tongue paralysis, aphasia and general paralysis.

Description

technical field [0001] The present invention relates to the technical field of medicine, in particular, the present invention relates to a preparation method and medical use of effective fractions of polypeptides in insects of the genus Parischnogaster spp. The effective part is obtained by dialysis of the crude venom of Vespa genus Vespa with a 25KDa dialysis membrane, and the eluate is freeze-dried or concentrated and dried under reduced pressure. Through a series of pharmacological and pharmacodynamic experiments in animals, the inventors found that the effective part of the polypeptide has the effect of significantly preventing and treating cerebral thrombosis, restoring the activity ability of cerebral palsy animals, and reducing ischemic brain damage, and can be expected to be used in the preparation of anti-cardiac and cerebrovascular diseases. Vascular diseases include drugs, health care products, medical devices and daily chemicals for ischemic cerebrovascular disease...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61P7/02A61P9/10A61P25/00
Inventor 肖怀巫秀美王斌刘衡何苗赵昱
Owner DALI UNIV
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