Extraction method, detection method and application of total alkaloids in pagoda japonica

A technology of total alkaloids and extraction method is applied in the field of extraction of total alkaloids from Sophora japonica and can solve problems such as the reduction of bean root resources, and achieve the effects of easy control, high repeatability, and easy industrial production.

Active Publication Date: 2016-11-30
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of the gradual decrease of the root resources in the current market, and to provide an extraction method, a detection method and an application thereof for total alkaloids in Sophora japonica, so as to explore whether the medicinal material of Sophora japonica can be used as A substitute for bean root, providing a scientific basis for resource utilization and clinical application

Method used

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  • Extraction method, detection method and application of total alkaloids in pagoda japonica
  • Extraction method, detection method and application of total alkaloids in pagoda japonica
  • Extraction method, detection method and application of total alkaloids in pagoda japonica

Examples

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Effect test

Embodiment 1

[0022] Example 1 Extraction method of total alkaloids in pagoda japonica (method 1)

[0023] 1-1 Grind the multi-leaf pagoda japonica medicinal material into a 20-mesh coarse powder, reflux extraction with 70% ethanol at 90°C for 5 times, each time for 1 hour, filter while it is hot, and recover the solvent to obtain a compound with a relative density of 1.20 at 60°C Fluid extract: add water to suspend the fluid extract, add dropwise concentrated sulfuric acid until the pH value is 3; extract with chloroform until the color of chloroform becomes lighter, add potassium carbonate to the acidic water layer until the pH value is 11, extract with chloroform until the chloroform liquid is steamed There is no alkaloid reaction after drying, and the chloroform is recovered to obtain the chloroform extract, which is the total alkaloid.

[0024] 1-2 Crush the multi-leaf pagoda japonica medicinal material into 80-mesh coarse powder, reflux extraction with 75-90% ethanol at 85°C for...

Embodiment 2

[0026] Example 2 Extraction method of total alkaloids in pagoda japonica (method 2)

[0027] 2-1 Grind 20 kg of leafy pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolator, compact it layer by layer, and then put 160 kg of prepared 1% hydrochloric acid aqueous solution into the percolator, Sealed, extract for 7 days, do not stir during the percolation process, collect the percolate; pass the percolate through the pre-treated 732 cation exchange resin column, and then wash the resin column with water until the water is colorless; pour the resin into the open In a container, dry it in the air or dry it at 55-65°C, knead it with ammonia water, extract it with chloroform reflux 5 times, and evaporate the chloroform extract to dryness to check that there is no alkaloid reaction, and recover the chloroform extract to obtain the chloroform extract, namely Get total alkali.

[0028] 2-2 Grind 15 kg of leafy pagoda japonica...

Embodiment 3

[0033] Example 3 Determination method of total alkaloids in pagoda japonica

[0034] Get 10 mg of the total alkaloids of Pagoda japonica polyphylla obtained by the extraction method of Example 1 or 2, put it in a 10ml volumetric flask, add chloroform to the mark, shake it up, and use it as the test solution; Add 10ml of chloroform, 0.2ml of concentrated ammonia test solution, shake for 15 minutes, filter, evaporate the filtrate to dryness, add 0.5ml of chloroform to dissolve the residue, and use it as a reference medicinal solution; Reference substance of reference substance, oxidized sophocarpine reference substance plus chloroform to make a mixed solution containing 1mg per 1ml, as the reference substance solution, test according to thin-layer chromatography, draw the test solution, and control medicinal material solution 1~ 2 μl, 4~6 μl of the reference substance solution, respectively spot on the same silica gel G thin-layer plate, use chloroform-methanol-concentrate...

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Abstract

The invention discloses an extraction method, a detection method and an application thereof for total alkaloids in pagoda japonica, wherein the extraction methods are divided into two types: ① heating and refluxing the medicinal material of pagoda japonica with 70-95% ethanol to obtain a liquid extract , after adding water suspension and adjusting acid treatment, extracting with chloroform to finally obtain the total alkaloids; ② Firstly extract the leafy pagoda japonica medicinal material by percolation, then extract by elution with 732 cation exchange resin, and finally elute the resin with chloroform to obtain total alkaloids. The obtained total alkaloids were detected by thin-layer chromatography, using chloroform-methanol-concentrated ammonia test solution (4:1:0.1) as the developer, and matrine, oxymatrine and oxysophocarpine as the total Qualitative components in alkaloids, the results are accurate and reliable. The extraction process of the invention is simple, and the obtained total alkaloids have pharmacological effects such as anti-gastric ulcer, anti-coagulation, anti-cerebral ischemia, anti-thrombosis and lowering blood fat, and have great market development prospects.

Description

technical field [0001] The invention belongs to the technical field of medicinal liquid extraction, and in particular relates to an extraction method, a detection method and an application of total alkaloids in pagoda japonica. Background technique [0002] Leafy Sophora japonica Sophora tonkinensis Gagnep.var. polyphylla S. Z. Huang et Z. C. Zhou It is a plant of the genus Sophora in the leguminous family, and it is combined with the commonly used Chinese medicinal material Shandou root [scientific name: Vietnam Sophora japonica Sophora tonkinensis Gagnep] is the root of the same family, genus and species, and it is a variant of Sophora japonica. It grows in Shishan District, and is distributed in Guangxi, Guangdong, Yunnan, Guizhou and Jiangxi provinces. In Guangxi, it is mainly distributed in the Hongshui River Basin, such as Du'an, Mashan, Xincheng, Wuming and other places. It is cold in nature, bitter in taste, and has the effects of clearing heat and detoxifyin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/48A61P1/04A61P7/02A61P9/10A61P3/06G01N30/90
Inventor 卢文杰陈家源陆国寿谭晓黄建猷黄周锋何开家李燕婧钟正贤
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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