48 results about "Sophora tonkinensis" patented technology

Compositions derived from traditional Chinese herbal medicines, medicinal plants and extracts thereof, are provided for the prevention and treatment of cancers especially lung cancer, prostate cancer, liver cancer, breast cancer and leukemia. The composition is also useful for treating Helicobacter pylori infection The composition is also useful for treating or preventing a chronic inflammatory condition. The composition is also useful for treating or preventing cardiovascular disease. The composition is also useful for treating or preventing cerebral vascular disease. The compositions are useful as adjuncts to conventional surgery, chemotherapy or radiotherapy treatments in patients with cancer. Preferred compositions of the invention contain the herbal ingredients Sophora tonkinensis, Polygonum bistorta, Prunella vulgaris, Sonchus brachyotus, Dictamnus dasycarpus Turcz, and Dioscorea bulbifera.

The invention discloses a sophora tonkinensis endophytic fungus SDTE-P. The sophora tonkinensis endophytic fungus SDTE-P is classified and named as Penicillium citrinum SDTE-P, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10808. According to the sophora tonkinensis endophytic fungus SDTE-P provided by the invention, a strain of endophytic fungus Penicillium citrinum SDTE-P is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Penicillium citrinum SDTE-P has a strong inhibiting effect on panax notoginseng fusarium solani, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a medicament for treating acne rosacea and a preparation method thereof. The main medicinal components comprise 8 to 10 portions of paris polyphylla, 8 to 12 portions of spreading hedvotis herb, 6 to 10 portions of angelica, 6 to 10 portions of szechuan lovage rhizome, 6 to 10 portions of safflower, 8 to 10 portions of trogopterus dung, 4 to 6 portions of trumpet creeper, 4 to 6 portions of red paeony root, 6 to 10 portions of radix scutellariae, 6 to 10 portions of radix rehmanniae, 6 to 10 portions of glabrous greenbrier rhizome, 8 to 12 portions of sophora tonkinensis, 8 to 12 portions of loquat leaves, 6 to 8 portions of white mulberry root-bark, 8 to 12 portions of amur corktree bark, 6 to 8 portions of tree peony bark, 6 to 8 portions of gardenia, 6 to 8 portions of red paeony root, 3 to 5 portions of gypsum, 3 to 5 portions of common anemarrhena rhizome, 6 to 8 portions of rhubarb, 7 to 9 portions of immature bitter orange, 7 to 9 portions of forsythia, 7 to 9 portions of pangolin, and 6 to 10 portions of raw licorice; and the assistant medicinal components comprise 10 to 12 portions of orpiment, 10 to 20 portions of sulfur, and 20 to 30 portions of aviation kerosene. Through the comprehensive action of the main medicament and the assistant medicament, the medicament conditions the interior and treats the exterior, and achieves good treatment effect.

The invention provides a planting method of sophora tonkinensis, and relates to the technical field of plant cultivation. The planting method includes the following steps of (1) land selecting, (2) soil preparation, (3) cup seedling cultivating, (4) cup seedling transplanting, (5) fertilization managing, (6) putting flowering branch on frames and (7) preventing and treating disease and insect damage. According to the planting method, no chemical fertilizer is added in the fertilization process, organic fertilizer is used for fertilization and is complete in nutrition and environmentally friendly, properties of medicine materials are not changed, soil is loosened, and the capacity for water retention, heat preservation, ventilation and fertilizer retention is improved; cup seedling cultivation is carried out on seeds of sophora tonkinensis, which is beneficial for promoting healthy and fast growth of the seeds while protecting the seeds. The planting method is high in survival rate and growth quality and suitable for large-scale planting.

The invention discloses a sophora tonkinensis endophytic bacterium B21. The sophora tonkinensis endophytic bacterium B21 is classified and named as Burkholderia sp. B21, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10806. According to the sophora tonkinensis endophytic bacterium B21 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B21 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B21 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a sophora tonkinensis endophytic bacterium B29. The sophora tonkinensis endophytic bacterium B29 is classified and named as Burkholderia sp. B29, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10807. According to the sophora tonkinensis endophytic bacterium B29 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B29 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B29 has a strong inhibiting effect on panax notoginseng fusarium solani, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a sophora tonkinensis endophytic bacterium B22. The sophora tonkinensis endophytic bacterium B22 is classified and named as Microbacterium sp. B22, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 29 September 2015, and the depository number is CGMCC No.11463. According to the sophora tonkinensis endophytic bacterium B22 provided by the invention, a strain of sophora tonkinensis endophytic bacterium Microbacterium sp. B22 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Microbacterium sp. B22 has strong inhibiting effect on panax notoginseng fusarium solani, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a sophora tonkinensis endophytic fungus SDTE-P. The sophora tonkinensis endophytic fungus SDTE-P is classified and named as Penicillium citrinum SDTE-P, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10808. According to the sophora tonkinensis endophytic fungus SDTE-P provided by the invention, a strain of endophytic fungus Penicillium citrinum SDTE-P is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Penicillium citrinum SDTE-P has a strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention provides a plant disinfectant and a preparation method thereof. The plant disinfectant is composed of the following raw materials in percentage by weight: 13-16% of ilex purpurea, 10-14% of honeysuckle, 6-8% of radix isatidis, 9-13% of isatis indigotica leaves, 15-18% of andrographis paniculata, 10-18% of sophora tonkinensis roots and the balance of garlic. The preparation method comprises the following steps: weighing raw materials, removing impurities, crushing, mixing, soaking in distilled water (25-30 DEG C) for 3-4 days, decocting, cooling, and filtering. The plant disinfectant provided by the invention combines a variety of natural herbal plants as raw materials, and has the advantages of no environmental pollution, good sterilization effect, good safety, no toxicity, no residue and no harm to the human body. The preparation method of the plant disinfectant is simple in operation and suitable for wide application.

The invention discloses a sophora tonkinensis endophytic bacterium B22. The sophora tonkinensis endophytic bacterium B22 is classified and named as Microbacterium sp. B22, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 29 September 2015, and the depository number is CGMCC No.11463. According to the sophora tonkinensis endophytic bacterium B22 provided by the invention, a strain of sophora tonkinensis endophytic bacterium Microbacterium sp. B22 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Microbacterium sp. B22 has a strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a method for extracting and purifying cytisine from Sophora tonkinensis. The method comprises the following steps: extraction: a Sophora tonkinensis medicinal material is pulverized, an aqueous solution of an organic solvent is used for extraction with ultrasound, the extract is filtered, filtrate is condensed, and a macroporous resin column is used for separating a Sophora tonkinensis total alkaloid extract product; separation: the alkaloid extract product is absorbed and separated by silica gel, and recrystallization is carried out in order to obtain the product. The method has the advantages of simple process, simple operation, and low cost; the materials used for separation are simple to regenerate without toxicity and pollution; the method is suitable for industrial large-scale production.

The invention discloses a sophora tonkinensis endophytic bacterium B29. The sophora tonkinensis endophytic bacterium B29 is classified and named as Burkholderia sp. B29, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10807. According to the sophora tonkinensis endophytic bacterium B29 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B29 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B29 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses a Chinese medicinal honeyed pill for treating recurrent psoriasis during active stage. The honeyed pill is prepared from the following Chinese traditional medicines by weight: 30 parts of Rehmannia glutinosa, 15 parts of Salvia miltiorrhiza, 12 parts of Paeonia suffruticosa bark, 12 parts of Radix Arnebiae, 40 parts of Flos Lonicerae, 15 parts of Forsythia suspensa, 30 parts of Herba Taraxaci, 15 parts of Sophora flavescens, 15 parts of Paris polyphylla, 10 parts of Sophora tonkinensis root, 15 parts of Morus alba leaf, 18 parts of Chrysanthemum morifolium flower, 18 parts of Radix Paeoniae Rubra, 16 parts of Cnidium monnieri fruit, 10 parts of Xanthium sibiricum fruit, 15 parts of Zaocys dhumnades and 10 parts of Radix Glycyrrhizae. The medicines are used together to bring out the best in each other, so that the honeyed pill has effects of dispelling toxic wind, eliminating toxic heat, eliminating pathogens and treating diseases.

The invention relates to a traditional Chinese medicinal composition for treating chronic bronchitis. The traditional Chinese medicinal composition comprises the following components in parts by weight: 5-15 parts of Belamcanda chinensis, 6-10 parts of Sophora tonkinensis root, 3-6 parts of Lasiosphaera Seu Calvatia, 15-25 parts of Ilex purpurea, 10-20 parts of Prunella vulgaris,3-10 parts of Platycodon grandiflorum, 3-10 parts Rhizoma Cynanchi Stauntonii (Cynanchum stauntonii and/or Cynanchum glaucescens), 10-20 parts of Herba Ardisiae,3-10 parts of Radix Polygalae (Polygala tenuifolia and/or Polygala sibirica), 10-20 parts of Pseudostellaria heterophylla, 5-15 parts of Atractylodes macrocephala, 1-5 parts of Cornu Cervi Pantotrichum (Cervus nippon and/or Cervus elaphus), and 10-20 parts of Terra Frava Usta. The traditional Chinese medicinal composition provided by the invention is prepared according to the theory of traditional Chinese medicine science, can effectively treat chronic bronchitis, and has the advantages of low toxic and adverse side effects, low cost, ultrafine pulverization, and high availability of effective ingredients.

The invention discloses a sophora tonkinensis endophytic fungus TRXY-59-2. The sophora tonkinensis endophytic fungus TRXY-59-2 is classified and named as Rhexocercosporidium sp.TRXY-59-2, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 03 December 2014, and the depository number is CGMCC No.10109. According to the sophora tonkinensis endophytic fungus TRXY-59-2 provided by the invention, a strain of endophytic fungus Rhexocercosporidium sp.TRXY-59-2 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Rhexocercosporidium sp.TRXY-59-2 has a strong inhibiting effect on panax notoginseng fusarium solani, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention discloses an extraction method and application of oxymatrine in leafy sophora tonkinensis. The extraction method of oxymatrine in the leafy sophora tonkinensis comprises the following steps: carrying out percolation extraction, carrying out eluting extraction by virtue of 732 cation exchange resin, then carrying out chloroform eluting treatment on the resin to obtain total alkali, carrying out neutral alumina column chromatography separation, and finally eluting with a chloroform-methyl alcohol mixed solvent (100:0-95:5), thus obtaining oxymatrine. The extraction method of oxymatrine in the leafy sophora tonkinensis is simple in process and can be put into industrialization easily, and the obtained oxymatrine has better antiviral, anti-inflammation and anti-tumour effects and has a great market development prospect.

The invention discloses application of sophora tonkinensis gapnep lupanol, and particularly discloses application of the sophora tonkinensis gapnep lupanol to preparation of medicinal preparations fortreating tumors. The sophora tonkinensis gapnep lupanol provided by the invention has very good antitumor activity, can significantly inhibit growth of nasopharyngeal cancer cells, cervical cancer cells, esophageal cancer cells, liver cancer cells, laryngeal cancer cells, breast cancer cells and prostate cancer cells and inhibit metastasis of the nasopharyngeal cancer cells, and has a good application prospect in development into medicines for treating nasopharyngeal cancer and other diseases.

The invention discloses a sophora tonkinensis endophytic fungus TRXY-59-2. The sophora tonkinensis endophytic fungus TRXY-59-2 is classified and named as Rhexocercosporidium sp.TRXY-59-2, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 03 December 2014, and the depository number is CGMCC No.10109. According to the sophora tonkinensis endophytic fungus TRXY-59-2 provided by the invention, a strain of endophytic fungus Rhexocercosporidium sp.TRXY-59-2 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Rhexocercosporidium sp.TRXY-59-2 has a strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention provides a Sophora tonkinensis Gagnep. seedling substrate and a preparation method thereof. The substrate comprises the ingredients of garden soil, carbonated rice hull and a coated sustained/controlled release dedicated compound fertilizer among which the proportion ranges from 5:4.7:0.3 to 6:3.5:0.5. The substrate is rich in organic matter, nitrogen, phosphorus, potassium and mineral nutrient elements, is appropriate in physical properties and PH value, high in permeability and water-holding capacity, wide in material source, easy to process, and low in cost, and facilitates dispersive operation of farmers.

The invention discloses sophora tonkinensis endophytic fungus TRXY-34-1. The sophora tonkinensis endophytic fungus TRXY-34-1 has the type name of Fusarium solani TRXY-34-1, an ITS sequence of the Fusarium solani TRXY-34-1 is as shown in SEQ ID NO. 1, and the Fusarium solani TRXY-34-1 is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on May 13, 2015 and has the collection number of CGMCC No. 10767. According to the sophora tonkinensis endophytic fungus TRXY-34-1, the endophytic fungus Fusarium solani TRXY-34-1 is obtained through being separated from roots of a medicinal plant, i.e., Sophora tonkinensis and carrying out screening for the first time and has a powerful inhibiting action on Alternaria panax Whetzel, and thus a broad application prospect is brought to the field of biological control on panax notoginseng fungal diseases.

The invention discloses sophora tonkinensis endophytic fungus TRXY-34-1. The sophora tonkinensis endophytic fungus TRXY-34-1 has the type name of Fusarium solani TRXY-34-1, an ITS sequence of the Fusarium solani TRXY-34-1 is as shown in SEQ ID NO. 1, and the Fusarium solani TRXY-34-1 is collected by the General Microorganisms Center of China Committee for Culture Collection of Microorganisms, which is located at Institute of Microbiology, Chinese Academy of Sciences, 3#, 1# Courtyard, Beichen West Road, Chaoyang District, Beijing, on May 13, 2015 and has the collection number of CGMCC No. 10767. According to the sophora tonkinensis endophytic fungus TRXY-34-1, the endophytic fungus Fusarium solani TRXY-34-1 is obtained through being separated from roots of a medicinal plant, i.e., Sophora tonkinensis and carrying out screening for the first time and has a powerful inhibiting action on Colletotriehum gloeosporioides, and thus a broad application prospect is brought to the field of biological control on panax notoginseng fungal diseases.

The invention discloses a sophora tonkinensis endophytic fungus TRXY-46. The sophora tonkinensis endophytic fungus TRXY-46 is classified and named as Rhexocercosporidium sp.TRXY-46, wherein the ITS sequence of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 03 December 2014, and the depository number is CGMCC No.10108. According to the sophora tonkinensis endophytic fungus TRXY-46 provided by the invention, a strain of endophytic fungus Rhexocercosporidium sp.TRXY-46 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Rhexocercosporidium sp.TRXY-46 has a strong inhibiting effect on panax notoginseng colletotrichum gloeosporioides, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

The invention relates to a vegetative pesticide subprostrate sophora root tall-alkali micro-emulsion and its preparation method. The subprostrate sophora root tall-alkali with subprostrate sophora root as raw stock is used as the active ingredient of the vegetative pesticide subprostrate sophora root tall-alkali micro-emulsion. The subprostrate sophora root tall alkali contains plant alkaloid with multi-ingredient, which can efficiently kill pest on the crop, and the micro-emulsion is provided with good control effect for the resistant insect asparagus caterpillar, cabbage worm, cabbage moth, cotton leafworm, and etc.

The invention discloses a traditional Chinese medicine composition for treating sphagitis. The traditional Chinese medicine composition comprises the following raw materials in parts by mass: 12-25 parts of magnolia officinalis, 12-18 parts of sterculia lychnophora, 10-20 parts of liquorice, 6-12 parts of carpesium abrotanoides, 8-15 parts of dried waterlily leaves, 10-16 parts of sowthistle tasselflower herb, 10-18 parts of lonicera japonica, 6-12 parts of rust-coloured crotalaria herb with root, 15-25 parts of flos chrysanthemi indici, 7-13 parts of marshy betony herb, 5-8 parts of rhizoma cimicifugae, 12-16 parts of alpinia katsumadai, 9-15 parts of adiantum capillus-veneris, 10-15 parts of aloe and 9-13 parts of sophora tonkinensis. The traditional Chinese medicine composition for treating sphagitis, provided by the invention, has the efficacies of clearing away heat and toxic materials, invigorating spleen to eliminate dampness, tonifying the spleen and kidney, nourishing yin and purging fire, and promoting tissue regeneration and curing sore, can be orally taken directly to enter the body for treatment of sphagitis, thereby having the characteristics that the treatment time is short, the effect is rapid, the therapeutic effect is definite, the cure rate is high, the effective rate is high, relapse is not easy to occur, high safety is achieved and no toxic and side effect is caused.

The invention relates to the field of traditional Chinese medicine cultivation, and discloses a biological bacterial fertilizer for saffron crocus and a preparation method of the biological bacterial fertilizer. The biological bacterial fertilizer is prepared from, by weight, 35%-45% of microbial bacteria, 15%-25% of bentonite, 25%-30% of soybean meal and 10%-20% of auxiliaries, wherein the microbial bacteria are saffron crocus endophytic fungi and sophora tonkinensis endophytic fungi. The two microorganisms, namely, the saffron crocus endophytic fungi and sophora tonkinensis endophytic fungi are utilized, the yield of saffron crocus is increased, the content of effective components is increased, and meanwhile root rot of saffron crocus corms is prevented and treated; and meanwhile, due to the application of the biological bacterial fertilizer, the complicated planting operation process can be avoided, the labor and time cost is reduced, the yield and effective component content of the saffron crocus are increased, and the economic benefit of the saffron crocus is increased.