Extraction method and application of oxymatrine in leafy sophora tonkinensis

A technology of oxymatrine and an extraction method, applied in the directions of medical preparations containing active ingredients, digestive system, organic chemistry, etc., can solve problems such as the reduction of mountain bean root resources, achieve good growth of tumor cells, reduce production costs, Swap fast effects

Active Publication Date: 2014-10-08
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of the gradual decrease of the root resources in the current market, to provide an extraction method and application of oxymatrine in the multi-leaf pagoda japonica, in order to explore whether the medicinal material of the multi-leaf pagoda japonica can be used as Substitutes to provide scientific basis for resource utilization and clinical application

Method used

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  • Extraction method and application of oxymatrine in leafy sophora tonkinensis
  • Extraction method and application of oxymatrine in leafy sophora tonkinensis
  • Extraction method and application of oxymatrine in leafy sophora tonkinensis

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Embodiment 1

[0019] Example 1 Extraction process of oxymatrine in polyphylla japonica

[0020] The extraction method of oxymatrine in multi-leaf pagoda japonica of the present invention comprises the following steps:

[0021] (1) Grind 20 kg of multi-leaved pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolator, compact it layer by layer, and then put 160 kg of prepared 1% hydrochloric acid aqueous solution into the percolator, Airtight, extract for 7 days, do not stir during the percolation process, collect percolation liquid.

[0022] (2) Pretreatment of 732 cation exchange resin column: put the resin in a clean container, rinse with water until the drainage is clear. Soak the resin in water for 15 hours to fully expand the resin. Soak the resin with 5% HCl solution twice the volume of the resin for 4 hours, and stir it from time to time, then wash the resin with low-purity water until the solution P H is close to 4, then treate...

Embodiment 2

[0028] Example 2 Extraction process of oxymatrine in polyphylla japonica

[0029] The extraction method of oxymatrine in multi-leaf pagoda japonica of the present invention comprises the following steps:

[0030] (1) Grind 15 kg of leafy pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolator, compact it layer by layer, and then put 300 kg of prepared 1% hydrochloric acid aqueous solution into the percolator, Airtight, extract for 1 day, do not stir during the percolation process, and collect the percolation liquid.

[0031] (2) Pass the percolate through the pre-treated 732 cation exchange resin column, and then wash the resin column with water until the water is colorless. Used 732 cation exchange resin specifications, model and pretreatment method are the same as embodiment 1.

[0032] (3) Pour the resin into an open container, dry it in the air or dry it at 55-65 ° C, knead it to dissolve, and extract it twice with ...

Embodiment 3

[0034] Example 3 Extraction Process of Oxymatrine from Sophora japonica

[0035] The extraction method of oxymatrine in multi-leaf pagoda japonica of the present invention comprises the following steps:

[0036] (1) Grind 30 kg of leafy pagoda japonica medicinal material into coarse powder, pass through a 20-mesh sieve, put it into a percolator, compact it layer by layer, and then put 450 kg of prepared 1% hydrochloric acid aqueous solution into the percolator, Airtight, extract for 3-5 days, do not stir during the percolation process, and collect the percolation liquid.

[0037] (2) Pass the percolate through the pre-treated 732 cation exchange resin column, and then wash the resin column with water until the water is colorless. Used 732 cation exchange resin specifications, model and pretreatment method are the same as embodiment 1.

[0038] (3) Pour the resin into an open container, dry it in the air or dry it at 55-65°C, knead it with ammonia water, extract it with chl...

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Abstract

The invention discloses an extraction method and application of oxymatrine in leafy sophora tonkinensis. The extraction method of oxymatrine in the leafy sophora tonkinensis comprises the following steps: carrying out percolation extraction, carrying out eluting extraction by virtue of 732 cation exchange resin, then carrying out chloroform eluting treatment on the resin to obtain total alkali, carrying out neutral alumina column chromatography separation, and finally eluting with a chloroform-methyl alcohol mixed solvent (100:0-95:5), thus obtaining oxymatrine. The extraction method of oxymatrine in the leafy sophora tonkinensis is simple in process and can be put into industrialization easily, and the obtained oxymatrine has better antiviral, anti-inflammation and anti-tumour effects and has a great market development prospect.

Description

technical field [0001] The invention belongs to the technical field of medicinal liquid extraction, and in particular relates to an extraction method and application of oxymatrine in pagoda japonica. Background technique [0002] Leafy Sophora japonica Sophora tonkinensis Gagnep.var. polyphylla S. Z.Huang et Z.C.Zhou is a plant of the genus Sophora in the family Fabaceae, and it is combined with the commonly used Chinese medicinal material Shandougen [scientific name: Vietnam Sophora japonica Sophora tonkinensis Gagnep] is the root of the same family, genus and species, and it is a variant of Sophora japonica. It grows in Shishan District, and is distributed in Guangxi, Guangdong, Yunnan, Guizhou and Jiangxi provinces. In Guangxi, it is mainly distributed in the Hongshui River Basin, such as Du'an, Mashan, Xincheng, Wuming and other places. It is cold in nature, bitter in taste, and has the effects of clearing heat and detoxifying, reducing swelling and relieving throat....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D471/22A61P29/00A61P1/00A61P35/00A61P31/12A61P31/22A61P31/14
CPCA61K31/4375C07D471/22
Inventor 卢文杰陈家源陆国寿谭晓黄建猷黄周锋何开家
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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