6 results about "Chloroform methanol" patented technology

This invention relates to a method for preparing verbascoside and its application in promoting skin lipid production, belonging to the technical field of skin care and topical products. The method includes the following steps: Selecting dried fleshy stems of *Cistanche deserticola*, *Cistanche tubulosa*, *Cistanche salsa*, and *Cistanche salsa* (all belonging to the Orobanchaceae family), pulverizing them after pretreatment, and extracting them with 50%-80% ethanol aqueous solution under reflux for 2-4 times, 1-3 hours each time. The extracts are combined and concentrated under reduced pressure until no alcohol odor remains, obtaining a concentrated solution. The concentrated solution is passed through an AB-8 macroporous adsorption resin column, eluted sequentially with deionized water and 30%-50% ethanol aqueous solution to remove impurities, and then eluted with 60%-90% ethanol aqueous solution to remove the target component. The eluent is collected and concentrated under reduced pressure, purified by silica gel column chromatography using chloroform-methanol at a volume ratio of 5:1-2:1 as the eluent. The target fraction is collected, concentrated under reduced pressure, and freeze-dried to obtain the purified verbascoside. The beneficial effects of this invention are: verbascoside has a skin-protective effect and exhibits superior skin-care activity when applied to products.

The invention discloses a preparation method and application of a light-operated mitochondrial autophagy nano inducer based on thermosensitive liposome, and the preparation method comprises the following steps: (1) dissolving ICG and TPP-Ser in DMSO, carrying out ultrasonic treatment, dropwise adding into ultrapure water, carrying out room temperature stirring reaction, and carrying out centrifugal collection and washing to obtain TSI; (2) dissolving DPPC, DSPC and DSPE-PEG2000-iRGD in a chloroform-methanol mixed solvent, and carrying out rotary evaporation to remove the solvent so as to form a lipid membrane; and (3) adding an aqueous solution of TSI into the lipid membrane, and carrying out hydration and ultrasonic treatment to obtain a suspension. The light-operated mitochondrial autophagy nano inducer prepared by the invention is applied to precise treatment of cancers, and drug release and mitochondrial autophagy regulation are realized through near-infrared laser irradiation. The preparation method has the advantages of strong space-time controllability, high tumor targeting property, multi-mechanism synergistic anti-tumor effect and diagnosis and treatment integration capability, stable preparation process and convenience in clinical transformation.

The invention discloses an intelligent medical coupling agent as well as a preparation method and application thereof, and belongs to the technical field of medical materials. Comprising the following steps: dissolving SiO2 nanoparticles in deionized water, adding an HPTS dye and gamma-aminopropyltriethoxysilane, purifying and concentrating to obtain a pH response nanoprobe; the preparation method comprises the following steps: dissolving dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidyl glycerol and a therapeutic drug in a chloroform-methanol mixed solvent, and evaporating and drying to obtain a lipid film; adding a PBS (Phosphate Buffer Solution) into the lipid film, introducing perfluoropropane, carrying out rotary shearing emulsification, and collecting micro-bubbles to obtain drug-loaded ultrasonic response micro-bubbles; the preparation method comprises the following steps: adding the carbomer powder into deionized water, and then adding glycerol, triethanolamine and methylparaben to obtain basic gel; adding a pH response nanoprobe into the basic gel, adding drug-loaded ultrasonic response microbubbles after the fluorescence intensity CV value is less than or equal to 5%, and performing multi-stage gradient vacuum treatment to obtain the intelligent medical coupling agent. The intelligent medical coupling agent provided by the invention realizes targeted delivery of drugs and physiological status feedback of tissues.

The application discloses a kind of rapid detection methods of 23 kinds of carotenoids in food, using chloroform-methanol-water volume ratio 1:2:0.8 mixed solvent extraction, then add water and chloroform, without adding any salt compound, mixed solvent in chloroform-methanol-water volume ratio 2:2:1.8, automatically layered into water phase layer-organic phase layer, water phase HLB column, sequentially eluted with organic phase and chloroform, finally detected by high performance liquid chromatography-mass spectrometry, quantitative analysis.The application simplifies the extraction step, the quantitative detection limit is low, applied to the rapid detection and analysis of 23 kinds of carotenoids commonly seen in food, the recovery rate is as high as 80.1-98.7%, with high sensitivity and reliability.

The invention discloses a novel method for improving solubility of oleosin protein, and belongs to the technical field of food. The method disclosed by the invention comprises two steps of oleosin protein extraction and dissolution; the method comprises the following steps: mixing a plant oil body with a non-polar solvent with the volume 3-5 times that of the plant oil body for 15-30 minutes, then centrifuging for 20-30 minutes under the condition of 7000-9000 rpm, adding chloroform / methanol / water (4: 2: 1) with the volume 3-5 times that of the plant oil body into a precipitate, mixing for 10-30 minutes, centrifuging under the condition of 7000-9000 rpm, carrying out nitrogen blowing on the precipitate for 10-30 minutes, freeze-drying for 24-48 hours, adding an ethanol solution with the concentration of 50%-95%, and freeze-drying for 10-48 hours to obtain the plant oil. And mixing for 5 to 15 minutes to obtain an oleosin protein solution. Compared with the prior art, the prepared oleosin protein solution has the advantages that the use of ultrasonic waves, microwaves, urea and other denaturants is avoided, the spatial secondary structure of the protein is kept stable, and the solubility is improved to 0.5 mg / mL to 0.7 mg / mL.

This invention provides a method for preparing the organic compound cytochalasin D, belonging to the field of organic compound preparation technology. The method for preparing cytochalasin D specifically includes the following steps: S1. Obtaining mycelium through constant temperature culture for 20 days; S2. Expanding the culture of the mycelium in rice and extracting it, eluting with a methanol-water gradient to obtain an extract; S3. Preliminary separation of cytochalasin D, collecting fifteen eluent segments labeled Fr.1~Fr.15; S4. Taking Fr.5 from step S3 and performing silica gel column chromatography, switching to a chloroform-methanol mixed solvent gradient elution, collecting nine eluent segments labeled Fr.5.1~Fr.5.9; S5. Separating the target product, labeled Fr.5.8.1~Fr.5.8.10, where Fr.5.8.8 is the target product cytochalasin D. This invention significantly improves purity and yield, shortens production time, and reduces costs, providing a better solution for the industrial production of cytochalasin D.