328 results about "Chloroform methanol" patented technology

A process for preparing high-purity momordicoside from fructus momordicae includes such steps as pulverizing, reflux extracting in alcohol, filtering to obtain extract A, suspending it in water, extracting in ethyl acetate 1-3 times, recovering ethyl acetate mother liquid, extracting in n-butanol 1-3 times, recovering n-butanol to obtain extract B, passing it through polyamide column, water washing, concentrating to obtain coarse saponin, chromatography by silica gel column, eluting with chloroform-methanol-water, collecting coarse momordicoside V, chromatography, water washing, eluting with alcohol, and collecting product.

A purified preparation of Mangosteen alcohol, which includes steps: (1) first crush Mangosteen and add ethanol to get alcohol extract, (2) suspend the extract in water, extract by ethyl acetate, recover ethyl acetate to get ethyl acetate extract and take the mother liquor, (3) add HCL into the mother liquor and ethyl acetate for acid hydrolysis with heating, separate the layer of ethyl acetate after hydrolysis and adjust it neutral with lye, abstract ethyl acetate, decompress and recycle the solvent to get the extract containing Mangosteen alcohol, (4) conduct silica gel column chromatography, chloroform elution, and recovery, chloroform-methanol elution, collect the elutes, recycle the solvents to get the crude, (5) conduct re-crystallization on the crude to get Mangosteen alcohol with purity morn than 95%.

The invention discloses a method for extracting lipid from Chlorella sorokiniana CS-01 (CCTCC M209220), namely an ultrasonic-assisted chloroform / methanol extraction method. The method mainly comprises the following steps of: collecting algae, drying, extracting by using chloroform / methanol, performing ultrasonic crushing, washing, and drying. Through the certification, compared with a Soxhlet extraction method, an acid heat extraction method, and a chloroform / methanol extraction method, the method has the advantages that: the extraction ratio of the lipid is improved by 1.4, 1.7 and 0.5 timesrespectively; meanwhile, a lipid extraction phase is positioned under a cell debris solid phase, so the method is favorable for the subsequent direct separation of the lipid, and meets the requirements of mass industrialized production.

The invention provides a wool-fabric protease anti-felting method based on weak oxidation and cutinase pretreatment, which belongs to the technical field of dyeing and finishing of wool fabric in the wool-spinning industry. The method aims to overcome the defects that the chlorination-process for wool-felting prevention seriously pollutes environment and the single protease-method treatment is poor in fabric wettability and high in felting rate in order to achieve the aim of optimizing the protease treatment effect of the wool fabric. All-wool grey fabric is extracted in a chloroform-methanol or carbon tetrachloride-methanol solution to remove free oily impurities, is pretreated with hydrogen peroxide and cutinase in turn to improve the hydrophilicity of the fiber surface, and then is subjected to reduction processing in a protease solution so as to realize biological processing combined with wool enzyme reduction. The method makes the prior chlorination-process felting prevention replaced with a biological-method wool-felting prevention process, and the method not only can effectively improve the reduction rate and anti-felting effect of the wool fabric, and promote the improvement of fabric wettability, but also can effectively increase the dyeing depth of fabric, and reduce fiber damage in reduction processing, and is favorable for protecting ecological environment.

Belonging to the technical field of medicine extraction, the invention discloses a method for extraction of paclitaxel from taxus chinensis. The extraction method provided in the invention consists of: using a methanol solution to conduct warm soaking extraction of taxus chinensis bark or branch and leaf powder, then carrying out chloroform extraction, performing silica gel column chromatography twice, implementing elution with a chloroform-methanol gradient solution and an n-hexane-acetic acid gradient elution respectively, and removing polar impurities so as to obtain a paclitaxel crude product; and further, carrying out third normal phase column chromatography, taking chemically treated silica gel as a stationary phase to effectively separate paclitaxel, recrystallizing the paclitaxel obtained from the column chromatography, thus obtaining a paclitaxel pure product with purity of over 98% pure product, with a process transfer rate being more than 80%. All the reagents used in the extraction method belong to the industrial grade, and the method provided in the invention has the advantages of wide applicability, strong innovation, simple process, low separation cost, high product yield, thus having innovative production value for industrial separation and production of paclitaxel.

The present invention discloses a technique for separating out and purifying triptolide monomer from common threewingnut root. The technique is carried out according to the following technical steps: (A) heating and extraction: after ethanol or methanol is warmly soaked, dregs are removed; (B) solvent extraction: organic solvent is adopted to extract concentrated solution in order to enrich the product; (C) chromatographic column chromatography: with neutral alumina or silica gel as filler, dichloromethane-methanol or chloroform-methanol is used to carry out gradient elution, and cut fraction is collected by stages; (D) high-efficiency preparation and liquid phase separation; (E) crystallization: two-phase solvent crystallization is adopted to use petroleum ether-ethyl acetate, dichloromethane-methanol or methylene dichloride-ethyl acetate to crystallize the cut fraction collected by stages, so that the colorless spiculate crystal product of triptolide, the purity of which reaches over 99.5 percent, can be obtained. The technique, which has the advantages of simplicity, rapidness, little solvent consumption, economy, environment-friendliness and high yield, can prepare a large amount of the product for one time, thus having high economic and academic values.

The invention provides a preparation method of a standard radix pseudostellariae cyclopeptide B. The preparation method comprises the steps of extracting and separating standard radix pseudostellariae cyclopeptide B from traditional Chinese medicine radix pseudostellariae, and purifying standard radix pseudostellariae cyclopeptide B; smashing the dry medicinal material of radix pseudostellariae, and then, carrying out heat reflux extraction on the dry medicinal material of radix pseudostellariae by using industrial ethanol to obtain an extract; pretreating the extract by using ethanol; enriching radix pseudostellariae cyclopeptide B through a silica gel column chromatography, wherein a chloroform and methanol system is used as an eluting solvent; finally, preparing radix pseudostellariae cyclopeptide B with the purity of more than 98% by using a preparative high performance liquid chromatography. The method provided by the invention can be used for rapidly separating radix pseudostellariae cyclopeptide B and is low in sample loss, relatively low in cost, convenient to operate, good in controllability and repeatability and suitable for industrial production; in addition, the solvent can be repeatedly recycled.

The invention relates to a Mucor circinelloides strain with high grease yield and application thereof. The invention discloses a Mucor circinelloides strain WJ11 and application thereof (a method for producing grease by using the strain). The method comprises the following steps: activating the strain to obtain an activated strain solution, carrying out seed culture under sterile conditions to obtain a seed culture solution, carrying out fermentation culture to obtain a fermentation culture solution, filtering, carrying out freeze-drying to constant weight to obtain a grease-containing Mucor circinelloides WJ11 dry strain, grinding, and extracting grease by a chloroform-methanol process. The Mucor circinelloides strain WJ11 has the advantages of high growth rate and high fatty acid content (up to 30-40% of biomass); the fatty acid content is 2-3 times of the control strain; and the biochemical analysis result shows that the Mucor circinelloides strain WJ11 has obviously higher enzyme activity related to fatty acid accumulation than the control group. The Mucor circinelloides strain has very wide application prospects when being used in microbial grease-producing industry and microbial grease accumulation analysis.

Disclosed are: a fat-reduced soymilk having a reduced fat content, which is produced by separating a fat from a fat-containing soybean efficiently without relying on an organic solvent; and a novel fat-rich soybean material. Specifically disclosed are: a fat-reduced soybean protein material characterized by containing a protein and a carbohydrate at a total content of 80 wt % or more in terms of dried form content, containing a fat (as an extract from a chloroform / methanol mixed solvent) at a content of less than 10 wt % relative to the content of the protein, and containing campesterol and stigmasterol (as plant-derived sterols) at a total content of 200 mg or more relative to 100 g of a fat; and a soybean emulsion composition characterized by containing a protein at a content of 25 wt % or more in terms of dried form content, containing a fat at a content of 100 wt % or more in terms of dried form content relative to the content of the protein in terms of dried form content, and having an LCI value of 60% or more.

The invention relates to a method for purifying huperzine A, which is easy for industrialized production. The method comprises the following production steps of: crushing of raw materials, acid water heating and extraction, membrane filtration, impurity removal and condensation, aminated chloroform extraction and condensation, medium-pressure alumina column chromatography, chloroform methanol recrystallization, and drying of a finished product. The method for producing the huperzine A has low energy consumption and short period.

A method for the preparing 20 (R)-ginseniside Rg3 includes dissolving panax notoginseng saponins (PNS) with water, adding acids (hydrochloric acid, phosphoric acid, sulfuric acid, acetic acid, glacial acetic acid, formic acid and the like) to adjust the pH to be 1 to 3, heating and returning the mixture for 30 minutes to 60 minutes, recycling an acid liquor and concentrating the acid liquor into paste, and drying the pasted liquor to obtain coarse converted products; and performing silica-gel column chromatography on the coarse converted products, performing gradient elution on chloroform-methanol-water, collecting an eluent, performing tracking detection through thin layer chromatography (TLC) compared with the ginseniside Rg3, merging flow parts mainly containing the ginseniside Rg3, recycling solvents with the amount two times of the amount of the pseudo-ginseng saponin before transformation, performing standing and filtration, and drying filter cakes to obtain the 20 (R)-ginseniside Rg3. Tested by high performance liquid chromatography (HPLC), the content of the 20 (R)-ginseniside Rg3 is larger than 90%. The method for preparing the 20 (R)-ginseniside Rg3 has the advantages of being simple, low in cost, high in product purity, and suitable for industrial production.

The invention discloses a method for determining the fatty-acid components of tissues of a fish body. The method comprises the following steps: taking the following tissues of the fish body in percentage by weight: 0.2-0.5% of abdominal fat, 0.8-1.0% of liver, 1-2% of muscle and 1.5-1.8% of gill, and then adding chloroform methanol mixing liquid for grinding; then extracting fat solution by returning and filtering; then blow-drying the solution by utilizing nitrogen and extracting crystallized fat; then dissolving the crystallized fat in potassium hydroxide methanol solution, introducing nitrogen for resisting oxidation and cooling the wall of a container for reducing the volatilization; then esterifying the fat into the fatty acid, then dissolving the fatty acid by using n-heptane; and then taking supernate for centrifugal treatment, then taking and conveying supernate into a gas chromatograph to obtain a chromatogram, and calculating the relative contents of the fatty acid components by the chromatogram through an area normalization method. Therefore, the method disclosed by the invention has the advantages that the recognition for the fat structure of the fish can be realized, so that the fish can be further protected and cultured.

The invention relates to a method for preparing catalpol, which has simple operation, light pollution and less equipment investment. The method comprises the following processing steps of: taking fresh roots of rehmannia root; adding methanol of which the volume is 5 to 10 times weight of the fresh roots; refluxing for 2 to 4 times, and taking 0.5 to 2 hours for each refluxing; merging backflow liquid; filtering the liquid; recovering the methanol at reduced pressure and condensing the liquid; adding isopyknic water saturation butarol to extract for 3 to 8 times; merging a butarol layer; recovering the butarol at reduced pressure until the butarol is exhausted; adsorbing the butarol with a macroporous adsorbent resin column; carrying out water washing to remove impurities; carrying out elution with 5 to 30 percent ethanol; collecting eluent of which the volume is 3 to 8 times volume of the column; concentrating and drying the eluent; adding the eluent to a silica gel chromatographic column by a dry method; carrying out elution with chloroform-methanol-water (6:4:1); making the eluent in each column volume as one fluid part; collecting 3rd to 6th fluid parts; merging the fluid parts; recovering the solvent and concentrating the fluid parts; and adding anhydrous ethanol to crystallize. The catalpol prepared by the method has high product purity and is easy to realize industrialization expansion.

This invention discloses a method for separating vitexin derivatives, vitexin-4'-O-glucoside and vitexin-2''-O-glucoside. The method comprises: (1) pulverizing hawthorn leaves into coarse powder, extracting by refluxing in alcohol aqueous solution, filtering, incorporating the filtrates, vacuum-concentrating to no alcohol odor, removing impurities with macroporous adsorption resin AB-8 or D101, eluting with 30-80% ethanol, vacuum-concentrating to no alcohol odor, decolorizing with petroleum ether, extracting with n-butanol, vacuum-recovering n-butanol, separating the extract by silica gel column chromatography, eluting with chloroform-methanol at a ratio of (4-3):1, vacuum-concentrating the eluate, separating by silica gel column chromatography,, eluting with ethyl acetate-butanone-methanoic acid-water or ethyl acetate-acetone-methanoic acid-water, and standing at 0-15 deg.C to obtain vitexin-4'-O-glucoside and vitexin-2''-O-glucoside. The method has such advantages as high yield, high purity, simple process and low toxicity, and is environmentally friendly.

The invention is a method to separate main monomer components in soy isoflavone, using the mixture with not less than 40% soy isoflavone content as raw material, silica gel as sorbent, and chloroform-methanol solution as eluting system, its technical steps: preparing sample solution; processing sorbent and placing in column; applying sample, where the added quantity of sample solution is 5-15% of effective volume of separating column; eluting, where the eluent flow velocity is controlled at 0.5-1.5% of effective volume of separating column each minute, and collecting the eluent in sections; qualitatively detecting the collected eluents, respectively and compared with the contrast product; obtaining monomer components, combining the eluents with the same component, and respectively vacuum condensing and drying to obtain four main monomer components: genistein, daidzein, genistin and daidzin. It is detected by high efficiency liquid phase chromatography that each monomer component's content can reach above 90%.

Production of medicinal component snow bile pigment and its usage for preparing medicines in treatment of cancers are disclosed. The process is carried out by taking snow bile plant tuber, crushing, taking acetone 18-26 proportion as solvent, extracting by diacolation method, recovering extract in water-bath kettle at 60-75 degree to obtain extractive, adding extractive into silica gel 1-2 proportion, drying to obtain silica-gel column, gradient eluting by ethanol or chloroform with volume concentration 2-10%, recovering eluent to obtain coarse product, heating while dissolving for ethanol or alcohol in water bath, filtering, lowering to room temperature, re-crystallizing to obtain prism crystal with purity above 98%. It can be used to prepare medicines in treatment of hepatocarcinoma, lung cancer, gastric carcinoma, laryngeal cancer and leukemia.

The invention relates to a refined sweetsop total lactone for anticancer and a method for preparing the same. The method comprises that: Root, leaf and bark or seed of sweetsop are used as raw materials, are percolated and extracted by petroleum ether, chloroform, and acetic ether, or a mixed solution of the petroleum ether, the chloroform and acetic ether, or the raw materials are extracted by supercritical carbon dioxide; the raw materials adopts solvent method, namely the raw materials are heated and dissolved by the petroleum ether and are subjected to impurity removal to obtain sweetsop total lactone; and the sweetsop total lactone is subjected to silica gel column chromatography, is subjected to gradient elution by the petroleum ether-the acetic ether or the chloroform-methanol, and is separated and is refined to obtain the sweetsop total lactone taking Shikemoxin and valeric sweetsop as main compositions. The raw materials have rich sources, low price and availability; the obtained refined sweetsop total lactone has high content, clear compositions and controllable quality; and the preparation method has a simple process, low consumption and high yield, and can realize large industrialized production.

The invention discloses a selfheal quality control method which is a high performance liquid chromatography and a thin layer chromatography the marker components of which contain compound salviaflaside. The high performance liquid chromatography comprises the following steps: preparation of comparison products: carbinol is used to dissolve the salviaflaside compound so as to prepare the comparison product solution of the salviaflaside compound; preparation of test product solution : selfheal medicinal material powder is taken to be precisely weighed, added with carbinol, and filtered by a microfiltration membrane to be used as the test product solution; and injection to a liquid chromatograph, determination and mobile phase: elution by acetonitrile acetic acid liquid. The thin layer chromatography comprises the following steps: rosmarinic acid of the comparison product solution and salviaflaside solution and the test product solution are respectively absorbed to be sampled on the same thin board, chloroform:carbinol:formic acid or chloroform: water are taken as developers, developing, taking and drying are carried out, vanillin concentrated sulphuric acid is used for developing, and heating is carried out until spots are clear. The method is simple and effective, and thereby preventing illegal producers from using roots, stems, leaves and other non-medical parts to substitute the cluster parts of the selfheal to be used for the medical purpose.

The invention provides an amino acid ionic liquid water-based additive and a preparation method and application thereof, belonging to the technical field of organic compounds of lubricating oil additives. The structural formula of the amino acid ionic liquid water-based additive provided by the invention is shown as a formula 1 which is described in the specification. The amino acid ionic liquid water-based additive provided by the invention is biodegradable, environment-friendly and pollution-free, and has good solubility. The prepared amino acid ionic liquid water-based additive can also befully dissolved in organic solvents with different polarities, such as chloroform, methanol and water, and has excellent anti-friction and anti-wear properties and high bearing capacity when used as alubricant of a steel / steel friction pair.

The invention provides a method for purifying arecoline, which comprises the steps: taking the arecoline material, removing the core, crushing, adding 5-10 times of alcohol or methanol solution with the concentration of 30-50%, refluxing and extracting for 2-3 times, recovering a reagent from an extracting solution, adjusting the pH of a concentrated solution by hydrochloric acid to be 3-5, adding macroporous resins for absorption, readjusting the pH of a lower column liquid to be 7-8, adding chloroform for extraction, using the extracting solution to recover the chloroform to obtain an oily betel nut extract, selecting a chloroform-methanol-water solvent system or a normal hexane ethyl acetate methanol-water solvent system, adopting a high-speed counter current chromatograph for separation, freezing, drying to obtain the high-content arecoline. The method adopted for producing the arecoline has the advantages of short production cycle and low cost.

The invention discloses an alkaloid dimer, a preparation method thereof and application of the alkaloid dimer as an antiviral agent. The method comprises the following steps: firstly, carrying out strain culture on fungus Pestalotiopsis sp.(ZJ-2009-7-6) during preparation, and carrying out fermental cultivation on the fungus; leaching the obtained mycelium with a chloroform-methanol mixed liquid (1 to 1) for three times, carrying out vacuum concentration, and extracting with ethyl acetate for three times, so as to obtain crude extractum; and sequentially carrying out positive slica column chromatography, Sephadex LH-20 gel column chromatography and HPLC (high-performance liquid chromatography) on ethyl acetate-phase crude extractum to obtain a compound shown in a formula I. The antiviral agent provided by the method has the characteristics that the compound shown in the formula I or a pharmaceutically acceptable salt is taken as an effective component for preventing and / or treating diseases caused by herpes simplex virus I (HSV-1) and / or enterovirus 71 (EV71).

The invention provides a method for quickly and efficiently purifying oleuropein. The method comprises the following steps of: smashing olive leaves, adding a 70-90 percent ethanol solution in an amount of 5-10 times that of the olive leaves for refluxing and extracting for 2-3 times, adding active carbon into an extracting solution for decoloring, filtering the active carbon out, recovering ethanol, adding water-saturated n-butyl alcohol into a concentrated solution for extracting for 3-5 times, collecting a n-butyl alcohol layer, and recovering under reduced pressure to obtain an extract; and separating the extract with a chloroform-methanol-n-butyl alcohol-water solvent system ((3-6):(2-4):(2-3):(1-2)) by using a preparation type high-speed counter current chromatograph, collecting active ingredients, and drying under reduced pressure to obtain high-content oleuropein. Due to the adoption of the method for purifying the oleuropein, the obtained product has high content, large preparation amount and low cost.

The invention relates to a method for preparing a compound of 2, 4-dihydroxy-5-methyl-hypnone by separating the fermentation liquid of Polyporus picipes. In the method, a method of plate-to-flask liquid culture is adopted; firstly, peeled potato, glucose and distilled water with the pH of 7.2 approximately are sterilized and made into a culture medium; then the culture medium is cultured and fermented to obtain the fermentation liquid; the fermentation liquid is extracted by ethyl acetate to obtain extractum; the extractum is separated by column chromatography and is eluted by a chloroform-methanol system in a gradient way; and the pure compound of the 2, 4-dihydroxy-5-methyl-hypnone (II) is obtained after several times of silica gel column chromatography, reverse C18 column and Sephadex LH-20 gel column chromatography and separation by a recrystallization method. The invention can develop the 2, 4-dihydroxy-5-methyl-hypnone (II) into the original drug of antipathogen fungi antibiotic and solves the problem of the raw material source of the 2, 4-dihydroxy-5-methyl-hypnone, and the invention has the advantages of mild culture condition, being simple and easy for confecting the culture medium, and short fermentation time, etc.

The invention relates to a process for the isolation of compound scopoletin which is used as nitric oxide synthesis inhibitor from Artemisia annua and other plant families, said process comprising extraction of dried powdered material of different plant parts with an aqueous acetonitrite solvent in the ratio of 1:5 for 6 to 8 hrs., concentration of the extracted solvent upto 30% of its original extract under vacuum, partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, drying halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, crystallizing the residues in methanol and filtering the crystals, concentrating the filtrate and chromatographed on silica gel, eluting scopoletin in chloroform methanol mixture; and crystallization of the fractions containing the scopoletin to get the pure scopoletin compound.

A general flavone of lilac daphne root is prepared from lilac daphne root through breaking, proportionally mixing it with alcohol, extracting at 40-70 deg.C for 12-48 hr, removing deposit, adsorbing by macroreticular resin, water washing, eluting with alcohol, vacuum distilling, passing through silicon gel column, eluting with the mixture of chloroform and methanol, and concentrating. It can be used to prepare the medicines for preventing and treating the sexual tumor.

The invention relates to a high-performance liquid chromatography of phosphatidylserine. The High-performance liquid chromatography comprises the steps of (1) preparing a phosphatidylserine standard solution, and using a chloroform-methanol solution to dissolve and fix the constant volume to be 80 to 400ug / ml; (2) preparing a phosphatidylserine sample solution, using the chloroform-methanol solution to dissolve and fix the volume, and diluting and preparing the sample solution of which the phosphatidylserine content is within the concentration range of standard serial solutions; (3) measuring the standard solution and the sample solution under the condition that the high-performance liquid chromatography cooperates with an evaporative light-scattering detector, performing logarithmetics on the peak area of the standard solution as a y-coordinate, performing logarithmetics on the concentration of the standard solution as an x-coordinate, drawing a double logarithmic standard curve, and finding the concentration of the sample solution from the double logarithmic standard curve according to the peak area. The high-performance liquid chromatography has the following beneficial effects: due to the fact that the high-performance liquid chromatography cooperates with the evaporative light-scattering detector, a simple and effective method is provided for the quality control during a phosphatidylserine production process through the entering of different mobile phase volume ratios.

The invention discloses an extraction method of Diosbulbin B, which comprises the following steps: carrying out reflux extraction on airpotato yam acetone, and concentrating the extracting solution until no acetone smell emits, thereby obtaining an extract; mixing the extract and silica gel, adding petroleum ether for extraction, drying filter residues by evaporation, adding chloroform for extraction, and drying the extracting solution by evaporation, thereby obtaining a chloroform part; mixing the chloroform part and silica gel, passing the mixture through columns, eluting with a mixed solution of chloroform and methanol in the volume ratio of 98:2, and collecting the eluent at multiple sections; recovering the solvent from the eluent, dissolving in acetone, crystallizing to obtain a crystal, passing the filtrate through columns, and eluting with a mixed solution of chloroform and methanol in the volume ratio of 99:1; and recovering the solvent from the eluent, dissolving in acetone, crystallizing, and merging the crystals to obtain the Diosbulbin B. In the invention, by selecting the extraction process and the eluents, the Diosbulbin B can be extracted, and the obtained extract does not contain other substances, thus further separation and purification are not needed. The method is relatively simple to operate, can be used for effectively extracting the Diosbulbin B, and has important meanings for further research on pharmacology and drug effects of the Diosbulbin B.

The process of measuring matrine content in Qingbai cleaning lotion includes the following steps: preparing reference substance solution of 0.01-0.1 mg / ml concentration with matrine and methanol; preparing sample solution with sample in 5-20 ml through adding concentrated ammonia solution in 0.25-1 ml, adding chloroform in 20 ml and shaking for over three times, merging the chloroform solutions, evaporating to dry, dissolving in chloroform, setting in alkali alumina column, eluting with chloroform-methanol solvent (2-10 to 0-2) in 10-50 ml, collecting and evaporating to dry the eluted solution, dissolving in methanol, transferring to a 50 ml measuring flask, diluting with methane to the scale, shaking and filtering with microporous filter film to obtain the filtrate; measuring the reference substance solution and sample solution of 5-40 microliter each in a liquid chromatograph and calculation to obtain the result. The present invention is fast and accurate.

The invention discloses a method for extracting microalgae oil through a compound enzyme. The method comprises the steps that the compound enzyme composed of helicase, cellulase and trypsin and surfactant are added to suspended algae slurry subjected to ultrasonic oscillation, microalgae cells are broken through enzymolysis to release oil, extraction is conducted through an extraction agent composed of chloroform methanol, and the oil extraction rate reaches 90% or above. According to the method for extracting the microalgae oil through the compound enzyme, the reaction condition is mild, energy consumption is low, the processing cost is low, and the method is suitable for large-scale industrial production of algae oil.