22 results about "Chloroform methanol" patented technology

The invention relates to a carbon and nitrogen dual-recovery method and device for collecting grease and phycocyanin in an algae-bacterium symbiotic sewage treatment system, and the method comprises the following steps: 1, carrying out mud-water separation on phycomycete suspension taken out from the sewage treatment system to obtain phycomycete biomass precipitate; 2, extracting grease in the precipitate in the first step by adopting a chloroform-methanol solution, and collecting the grease to recover carbon; and step 3, adding a hydrochloric acid solution into the biomass extracted in the step 2, heating and stirring, and collecting phycocyanin in supernate to recover nitrogen. According to the method disclosed by the invention, the mode of firstly collecting the grease to extract the carbon and then collecting the phycocyanin to extract the nitrogen is adopted, so that grease loss caused in the process of collecting the phycocyanin is avoided, the collection rate of the grease is improved, and dual recovery of the carbon and the nitrogen can be realized.

The invention discloses a preparation method of a composite spray for improving the hemostatic ability of a wound part, the composite spray comprises a liquid A and a liquid B. The liquid B is a sodium alginate aqueous solution, a preparation method of the solution A comprises the following steps: 1, dissolving a lipopeptide conjugate, cholesterol, distearoyl phosphatidylcholine, distearoyl phosphatidylserine and dimethyl sulfoxide in a chloroform-methanol mixed solution to obtain a mixed system; 2, preparing the mixed system into a precursor solution by utilizing a film hydration method; 3, adding calcium chloride into the precursor solution, and reacting in a dark place to obtain a solution A; a precursor solution is designed according to the function of natural platelets, the function of the precursor solution is equivalent to that of nano platelets, the precursor solution is matched with sodium alginate, adverse effects on a blood coagulation system caused by fibrin degradation, platelet activation capability reduction and the like of a wound part due to blood loss can be relieved in a targeted manner, and the preparation has the advantages of rapid hemostasis, good biological activity, no toxic or side effects and the like. And the wound healing is rapid.

The invention discloses an efficient microalgae resourceful treatment method which comprises the following steps: inoculating a microalgae concentrated solution with the dry weight of more than 30mg / L into wastewater to be treated, then respectively adding nano zero-valent iron suspensions with different concentrations at different physiological stages of microalgae, culturing for 14 days to obtain a microalgae culture solution, detecting the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution, and determining the content of the microalgae in the wastewater to be treated according to the indexes of COD, NH4 < + >-N and PO4 < 3->-P in the microalgae culture solution. The method comprises the following steps: standing a microalgae culture solution for 9 hours, carrying out self-flocculation settling on microalgae, carrying out centrifugal treatment on the microalgae culture solution subjected to self-flocculation settling, filtering, carrying out freeze drying to obtain microalgae powder, and extracting the microalgae grease by adopting a chloroform-methanol method. According to the method, nano zero-valent iron with different concentrations is added in different growth stages of the microalgae, so that the performance of removing nutrient substances in the wastewater by the microalgae is enhanced, and meanwhile, the flocculation capacity of the microalgae and the accumulation of grease content are promoted.

The invention discloses an in-situ dePEGylation nano drug delivery system as well as a preparation method and application of the in-situ dePEGylation nano drug delivery system. The preparation method comprises the following steps: adding EDC.HCl and NHS into DMF (Dimethyl Formamide) containing mPEG-COOH, and stirring at room temperature; dropwise adding the activated solution into a DMF (Dimethyl Formamide) solution containing MMP2 sensitive peptide, stirring overnight at room temperature, dialyzing and freeze-drying to obtain MMP2 sensitive peptide-PEG-COOH; continuously activating terminal carboxyl, adding MDM2 targeting peptide, stirring at room temperature, dialyzing and freeze-drying to obtain a PEG-MMP2 peptide-MDM2 peptide compound; dissolving the compound in chloroform / methanol, performing ultrasonic dissolution, sequentially adding Dox and ATO nanoparticles, and stirring to form an oil phase solution; and carrying out rotary evaporation to form a film, carrying out PBS resuspension and ultrasonic hydration, and carrying out dialysis and purification to obtain the nano drug delivery system. Peeling of the PEG layer is specifically triggered through a tumor microenvironment, and precise drug release and synergistic anti-tumor treatment are realized in combination with a multi-stage response mechanism.

This invention discloses a method for preparing a composite spray that improves hemostasis at wound sites. The composite spray includes solution A and solution B, where solution B is an aqueous solution of sodium alginate. The preparation method of solution A includes: 1. Dissolving a lipopeptide conjugate, cholesterol, distearylphosphatidylcholine, distearylphosphatidylserine, and dimethyl sulfoxide in a chloroform-methanol mixed solution to obtain a mixed system; 2. Preparing a precursor solution from the mixed system using a thin-film hydration method; 3. Adding calcium chloride to the precursor solution and reacting it in the dark to obtain solution A. This invention designs the precursor solution based on the function of natural platelets, and its function is equivalent to that of nanoplatelets. Combined with sodium alginate, it can specifically alleviate the adverse effects on the coagulation system caused by blood loss, such as fibrin degradation and reduced platelet activation, at wound sites. It has the characteristics of rapid hemostasis, good bioactivity, and rapid wound healing.

The application discloses a naringenin-antibacterial peptide composite liposome hydrogel and a preparation method and application thereof, and relates to the technical field of medicine, in particular to a naringenin-antibacterial peptide composite liposome hydrogel, which is prepared by the following steps: dissolving soybean lecithin, cholesterol and naringenin in a chloroform-methanol mixed solvent, rotary evaporation under reduced pressure, and vacuum solvent removal to obtain a naringenin-loaded lipid film; dissolving a bacillus subtilis antibacterial peptide in a PBS buffer solution to prepare a hydration solution, adding the lipid film to the hydration solution to rotate and hydrate to obtain a crude liposome suspension, and extruding the liposome suspension through polycarbonate membranes at room temperature to obtain a co-loaded liposome suspension; dispersing carbomer 940 in pure water, standing and swelling to prepare a gel matrix, mixing a humectant and a preservative into the gel matrix, and adding a neutralizing agent dropwise to adjust the pH value to 6.8-7.0; and mixing the liposome suspension and the gel matrix at room temperature, adding a PBS buffer solution to the mixture to prepare the naringenin-bacillus subtilis antibacterial peptide co-loaded liposome hydrogel, so as to solve the problems of skin irritation and bacterial drug resistance caused by long-term use of traditional acne treatment drugs.

The invention relates to the technical field of rhodanine therapeutic compounds, and discloses a preparation method and application of a rhodanine compound and a derivative thereof. The preparation method of the rhodanine compound and the rhodanine derivative comprises the following steps: mixing a compound A (0.3 mmol) and a compound B (0.3 mmol) in 3mL of acetonitrile at room temperature, then dropwise adding triethylamine (0.9 mmol), reacting the obtained mixture at 80 DEG C for 3 hours, cooling to room temperature, adding 10mL of ethyl acetate, and filtering to collect solids. And further dissolving the solid in 2mL of chloroform / methanol (with a volume ratio of 1: 2) solution, and passing through a Cl ion exchange column to obtain the product. The invention provides a rhodanine small molecule compound which is novel in structure and has an anti-klebsiella pneumoniae effect, and a derivative of the rhodanine small molecule compound.

This invention relates to a method for preparing verbascoside and its application in promoting skin lipid production, belonging to the technical field of skin care and topical products. The method includes the following steps: Selecting dried fleshy stems of *Cistanche deserticola*, *Cistanche tubulosa*, *Cistanche salsa*, and *Cistanche salsa* (all belonging to the Orobanchaceae family), pulverizing them after pretreatment, and extracting them with 50%-80% ethanol aqueous solution under reflux for 2-4 times, 1-3 hours each time. The extracts are combined and concentrated under reduced pressure until no alcohol odor remains, obtaining a concentrated solution. The concentrated solution is passed through an AB-8 macroporous adsorption resin column, eluted sequentially with deionized water and 30%-50% ethanol aqueous solution to remove impurities, and then eluted with 60%-90% ethanol aqueous solution to remove the target component. The eluent is collected and concentrated under reduced pressure, purified by silica gel column chromatography using chloroform-methanol at a volume ratio of 5:1-2:1 as the eluent. The target fraction is collected, concentrated under reduced pressure, and freeze-dried to obtain the purified verbascoside. The beneficial effects of this invention are: verbascoside has a skin-protective effect and exhibits superior skin-care activity when applied to products.

The invention provides a fluorescence detection method for single extracellular vesicles, which comprises the following steps: adding LPS (Lipopolysaccharide) into macrophages, and incubating to obtain polarized macrophages; extracting EV from the polarized macrophages; dissolving phosphatidylcholine in a chloroform-methanol mixed solvent, and carrying out rotary evaporation to obtain a liposome film; adding PBS and the mixed liquid medicine into the flask containing the lipidosome film, and mixing to hydrate the film; extruding the hydrated lipidosome through a filter membrane, centrifuging, and collecting the precipitate; dispersing the precipitate with pbs, centrifuging again, and taking the supernatant to obtain a liposome solution; adding DIL into the EV, incubating, centrifuging, collecting precipitate, and resuspending with PBS (Phosphate Buffer Solution); mixing the resuspension with a liposome solution, and incubating; and imaging by using an inverted fluorescence microscope. Fluorescence detection and analysis of internal information of the vesicles are achieved through membrane fusion of the lipidosome and the EV vesicles, and the problems of membrane structure damage, content leakage, high operation difficulty and the like caused by an existing method are solved.

A process enables efficient preparation of liposome-encapsulated peptide P33. The process comprising preparing a peptide P33; preparing a lipid mixture by dissolving 70-90 mg of phospholipid including granulated soy lecithin and 20 mg of cholesterol in 15-25 mg of a chloroform / methanol solvent system; evaporating organic solvent using a magnetic stirrer coupled with a heating system until a lipid film is fully formed and hydrated; removing solvent traces by drying the lipid film under vacuum conditions overnight; rehydrating the lipid film with 8-12 mL of a buffer containing 190-210 μg / mL of peptide P33; mixing the rehydrated lipid film thoroughly using a magnetic stirrer at ambient temperature to ensure uniform dispersion; sonicating the lipid suspension until a clear, translucent solution is obtained; and extruding the resulting suspension using a vortex to form large unilamellar vesicles (LUVs) with a uniform size of approximately 200 nm.

The invention belongs to the field of separation and extraction of Chinese medicinal materials, in particular to the invention, application and preparation of a new drug of gypenosanol B for antagonizing the side effects of radiotherapy, chemotherapy and glucocorticoid drugs. The existing drugs represented by glucocorticoid drugs show anti-inflammatory, anti-shock, anti-viral and immunosuppressive therapeutic effects in many diseases, but at the same time, the side effects caused to patients are also very large, mainly the problem that long-term use of glucocorticoids causes adrenal atrophy, sugar and salt metabolism disorders and damage to the heart, liver, lungs and kidneys. The following scheme is proposed, and the preparation method thereof comprises the following steps: S1, taking 20kg of gypenosanol total saponins, dissolving them in methanol, mixing them with 40kg of silica gel, and evaporating the solvent; S2, applying a 100kg dry silica gel chromatography column, and eluting them with a chloroform-methanol gradient. The drug of gypenosanol B extracted by the invention is used for antagonizing the side effects of glucocorticoid drugs and the side effects caused by radiotherapy and chemotherapy, and has a definite therapeutic effect and is safe and non-toxic.

The invention discloses a preparation method and application of a light-operated mitochondrial autophagy nano inducer based on thermosensitive liposome, and the preparation method comprises the following steps: (1) dissolving ICG and TPP-Ser in DMSO, carrying out ultrasonic treatment, dropwise adding into ultrapure water, carrying out room temperature stirring reaction, and carrying out centrifugal collection and washing to obtain TSI; (2) dissolving DPPC, DSPC and DSPE-PEG2000-iRGD in a chloroform-methanol mixed solvent, and carrying out rotary evaporation to remove the solvent so as to form a lipid membrane; and (3) adding an aqueous solution of TSI into the lipid membrane, and carrying out hydration and ultrasonic treatment to obtain a suspension. The light-operated mitochondrial autophagy nano inducer prepared by the invention is applied to precise treatment of cancers, and drug release and mitochondrial autophagy regulation are realized through near-infrared laser irradiation. The preparation method has the advantages of strong space-time controllability, high tumor targeting property, multi-mechanism synergistic anti-tumor effect and diagnosis and treatment integration capability, stable preparation process and convenience in clinical transformation.

The invention discloses an intelligent medical coupling agent as well as a preparation method and application thereof, and belongs to the technical field of medical materials. Comprising the following steps: dissolving SiO2 nanoparticles in deionized water, adding an HPTS dye and gamma-aminopropyltriethoxysilane, purifying and concentrating to obtain a pH response nanoprobe; the preparation method comprises the following steps: dissolving dipalmitoyl phosphatidylcholine, dipalmitoyl phosphatidyl glycerol and a therapeutic drug in a chloroform-methanol mixed solvent, and evaporating and drying to obtain a lipid film; adding a PBS (Phosphate Buffer Solution) into the lipid film, introducing perfluoropropane, carrying out rotary shearing emulsification, and collecting micro-bubbles to obtain drug-loaded ultrasonic response micro-bubbles; the preparation method comprises the following steps: adding the carbomer powder into deionized water, and then adding glycerol, triethanolamine and methylparaben to obtain basic gel; adding a pH response nanoprobe into the basic gel, adding drug-loaded ultrasonic response microbubbles after the fluorescence intensity CV value is less than or equal to 5%, and performing multi-stage gradient vacuum treatment to obtain the intelligent medical coupling agent. The intelligent medical coupling agent provided by the invention realizes targeted delivery of drugs and physiological status feedback of tissues.

The invention discloses a polyketone compound and application thereof, the compound is orange oily, can be easily dissolved in chloroform, methanol and DMSO, has a molecular formula of C21H32O3, and is obtained by fermentation, separation and extraction of Trichoderma citrinoviride, the obtained compound has an inhibition effect on methicillin-resistant staphylococcus aureus, and valuable compound resources are provided for development of new anti-MRSA drugs.

The application discloses a kind of rapid detection methods of 23 kinds of carotenoids in food, using chloroform-methanol-water volume ratio 1:2:0.8 mixed solvent extraction, then add water and chloroform, without adding any salt compound, mixed solvent in chloroform-methanol-water volume ratio 2:2:1.8, automatically layered into water phase layer-organic phase layer, water phase HLB column, sequentially eluted with organic phase and chloroform, finally detected by high performance liquid chromatography-mass spectrometry, quantitative analysis.The application simplifies the extraction step, the quantitative detection limit is low, applied to the rapid detection and analysis of 23 kinds of carotenoids commonly seen in food, the recovery rate is as high as 80.1-98.7%, with high sensitivity and reliability.

The invention discloses a novel method for improving solubility of oleosin protein, and belongs to the technical field of food. The method disclosed by the invention comprises two steps of oleosin protein extraction and dissolution; the method comprises the following steps: mixing a plant oil body with a non-polar solvent with the volume 3-5 times that of the plant oil body for 15-30 minutes, then centrifuging for 20-30 minutes under the condition of 7000-9000 rpm, adding chloroform / methanol / water (4: 2: 1) with the volume 3-5 times that of the plant oil body into a precipitate, mixing for 10-30 minutes, centrifuging under the condition of 7000-9000 rpm, carrying out nitrogen blowing on the precipitate for 10-30 minutes, freeze-drying for 24-48 hours, adding an ethanol solution with the concentration of 50%-95%, and freeze-drying for 10-48 hours to obtain the plant oil. And mixing for 5 to 15 minutes to obtain an oleosin protein solution. Compared with the prior art, the prepared oleosin protein solution has the advantages that the use of ultrasonic waves, microwaves, urea and other denaturants is avoided, the spatial secondary structure of the protein is kept stable, and the solubility is improved to 0.5 mg / mL to 0.7 mg / mL.

The application discloses a kind of microalgae efficient resource processing method, the method is in the wastewater required to be treated with the inoculation of microalgae concentrated liquid with dry weight greater than 30mg / L, then different physiological stages of microalgae are added with different concentrations of nano zero-valent iron suspension, after 14 days of culture, obtain microalgae culture solution, detect COD, NH4 + -N, PO4 3‑ -P index in microalgae culture solution, after 9h of microalgae culture solution, make microalgae self-flocculation settlement, centrifugal treatment is carried out to microalgae culture solution after self-flocculation settlement, after filtration, freeze-drying is carried out, obtain microalgae powder, then chloroform-methanol method is used to extract microalgae oil.The application adds different concentrations of nano zero-valent iron in different growth stages of microalgae, enhances the performance of microalgae to remove nutrients in wastewater, and promotes the flocculation ability of microalgae and the accumulation of oil content.

The preparation method comprises the following steps: (1) dissolving liposome in a chloroform-methanol mixed solvent, carrying out rotary evaporation to remove the solvent so as to form a lipid film, dispersing an antibacterial substance in a buffer solution so as to obtain a mixed solution, and drying the mixed solution so as to obtain a lipid film; adding the mixed solution into the dried lipid film, and carrying out vortex oscillation to form a multi-chamber liposome; (2) dissolving polyglutamic acid and a cross-linking agent in water, dropwise adding dimethyl sulfoxide, stirring, adding the multi-chamber liposome in the stirring process, slowly dropwise adding a catalyst, carrying out a catalytic reaction, and collecting solid particles after the reaction; and dispersing the solid particles in an adhesive to obtain the composite material for the antibacterial nursing apparatus. According to the composite material, polyglutamic acid is cross-linked to form a three-dimensional network structure, and liposome is embedded into the three-dimensional network structure, so that the release of an antibacterial agent can be further delayed, the antibacterial period is prolonged, and the requirement of frequently replacing instruments is reduced.

This invention provides a method for preparing the organic compound cytochalasin D, belonging to the field of organic compound preparation technology. The method for preparing cytochalasin D specifically includes the following steps: S1. Obtaining mycelium through constant temperature culture for 20 days; S2. Expanding the culture of the mycelium in rice and extracting it, eluting with a methanol-water gradient to obtain an extract; S3. Preliminary separation of cytochalasin D, collecting fifteen eluent segments labeled Fr.1~Fr.15; S4. Taking Fr.5 from step S3 and performing silica gel column chromatography, switching to a chloroform-methanol mixed solvent gradient elution, collecting nine eluent segments labeled Fr.5.1~Fr.5.9; S5. Separating the target product, labeled Fr.5.8.1~Fr.5.8.10, where Fr.5.8.8 is the target product cytochalasin D. This invention significantly improves purity and yield, shortens production time, and reduces costs, providing a better solution for the industrial production of cytochalasin D.

The present invention provides a fluorescence detection method for a single extracellular vesicle, the steps are as follows: LPS is added to macrophages, incubated, polarized macrophages are obtained; EV is extracted from polarized macrophages; phosphatidylcholine is dissolved in chloroform-methanol mixed solvent, rotary evaporated, and liposome film is obtained; PBS and mixed liquid are added to the flask containing liposome film, mixed, and the film is hydrated; after hydration, the liposome is squeezed and centrifuged through a filter membrane, and the precipitate is collected; after the precipitate is dispersed with pbs, it is centrifuged again, the supernatant is taken, and the liposome solution is obtained; DIL is added to EV, centrifuged after incubation, the precipitate is collected, and resuspended with PBS; the resuspended liquid is mixed with the liposome solution, incubated; imaging is performed with an inverted fluorescence microscope. The present invention realizes fluorescence detection and analysis of the internal information of the vesicle by the membrane fusion of liposomes and EV vesicles, avoiding the problems such as membrane structure damage, content leakage, and difficulty in operation brought by the existing method.

The invention discloses separation and application of brevifolin in Ophiopogon japonicus. The separation method comprises the following steps: taking the Ophiopogon japonicus as a raw material, obtaining an extract of the Ophiopogon japonicus, dispersing the extract in water, extracting with petroleum ether, putting the petroleum ether part on a silica gel column, carrying out gradient elution with chloroform-methanol, and purifying an obtained crude product of the Ophiopogon japonicus through reversed-phase high-performance liquid chromatography, wherein the chromatographic conditions are as follows: a chromatographic column takes octadecyl bonded silica gel as a filling agent; and the mobile phase is composed of methanol and water in a volume ratio of (5-95): (45-55). Pharmacological studies prove that the brevifolin has good anti-inflammatory activity, the half inhibitory concentration IC50 value of the brevifolin for inhibiting release of nitric oxide of RAW264.7 cells induced by LPS is 55.77 + / -7.28 mu M, and an important candidate compound is provided for developing novel anti-inflammatory drugs.

The present invention provides a schizophyllum bitter compound and a preparation method thereof. The structure of the schizophyllum bitter compound of the present invention is: The separation and preparation method of the compound of the present invention is to segment the ethyl acetate extract of the calyx of Lantern Festival with a normal phase silica gel column, elute with chloroform: methanol (90: 1-0: 1), and then perform normal phase column chromatography with chloroform: methanol (1: 0-20: 1) to elute the obtained 90: 1 fraction, and then perform gel column chromatography, ODS column chromatography, and semi-preparative HPLC to separate and purify. The present invention provides a theoretical basis for further enriching the research on the components of the calyx of Lantern Festival, and the preparation method provided is simple in steps and has strong operability.