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Application of sophora tonkinensis endophytic bacterium B29 in preventing and controlling panax notoginseng black spot

An endophytic bacteria and endophyte technology, applied in the biological field, can solve problems such as threats to health, pollution, and pesticide residues in Panax notoginseng products, and achieve the effect of strong inhibition and broad application prospects.

Active Publication Date: 2016-04-06
GUANGXI UNIV
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

In this new method for creating plants that are resistant against diseases caused by insect-borne fungi called Pygospora solani or Phytis nigricans. It involves separating these harmful microorganisms away from their roots while still allowing them to grow inside it's own cells without causing any damage during production process.

Problems solved by technology

Biology offers ways to fight against various types of microorganisms like yeast rust fungi, nematodes, phytoalexin producing bacillis, barrel shoe borne spores, root rods, stem corrosion, excessive nutrients consumption during crop rotation with increased risk of soilborrification, reduced productivity, environmental concerns associated with these treatments, and potential negative impact upon human wellnesses and safety issues related to food security management.

Method used

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  • Application of sophora tonkinensis endophytic bacterium B29 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic bacterium B29 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic bacterium B29 in preventing and controlling panax notoginseng black spot

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Isolation, screening and identification of strains

[0023] 1. Isolation of strains

[0024] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0025] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0026] Medium: 1000ml NA medium: 3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, 15g of agar, pH7.0.

[0027] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dr...

Embodiment 2

[0079] Inhibitory effect of metabolites of endophytic bacteria B29 from Sophora vietnamese against Panax notoginseng black spot

[0080] 1. Fermentation of strains and extraction of metabolites

[0081] Inoculate the endophytic bacteria B29 of Sophora vietnamese into a 2-liter Erlenmeyer flask containing 1000ml of NB liquid medium (3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, pH7. 130r / min shaking table fermentation culture for 10 days, the obtained fermented product was ultrasonically ultrasonicated for 40min, and then extracted twice with ethyl acetate, and the obtained ethyl acetate phase was concentrated under reduced pressure twice to obtain the crude ethyl acetate extraction of the fermented product of the strain The product is the metabolite of endophytic bacteria B29 in Sophora vietnamese.

[0082] 2. Inhibition of the metabolites of endophytic bacteria B29 from Sophora vietnamese on the mycelial growth of Panax notoginseng black spot fungus...

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Abstract

The invention discloses a sophora tonkinensis endophytic bacterium B29. The sophora tonkinensis endophytic bacterium B29 is classified and named as Burkholderia sp. B29, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10807. According to the sophora tonkinensis endophytic bacterium B29 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B29 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B29 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

Description

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Claims

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Application Information

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Owner GUANGXI UNIV
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