18 results about "Microbacterium sp." patented technology

The invention discloses a composite ecological restoration technology suitable for an open-pit coal mine waste dump. The technology comprises the following steps: (I) planting water-saving and convergence-increasing plants: sowing the water-saving and convergence-increasing plants to the open-pit coal mine waste dump to be restored in a shrub and grass seed broadcast sowing manner; (II) preparation and spraying of a prokaryotic microbial strain: culturing the separated and purified pseudoarthrobacter sp.xwn1, microbacterium sp.xwn2 and Acinetobacter sp.xwp1, carrying out enlarged culture on the cultured microbial agent, and then spraying and applying the microbial agent to the open pit coal mine waste dump after the microbial agent is applied to the open pit coal mine waste dump after the microbial agent is applied to the open pit coal mine waste dump after the microbial agent is applied to the open pit coal mine. By using the composite ecological restoration technology, the carbon sequestration capacity of plants is promoted, the organic carbon content of soil is increased, and ecological carbon sequestration restoration of the open pit coal mine waste dump is facilitated.

The application discloses a triazine herbicide propoxur degrading bacterial strain and application thereof, the bacterial strain is SWFU-DH02 strain which is negative in gram staining reaction and is identified as Microbacterium sp. Exiguobacteruim sp. ) on November 22, 2021, and is deposited in the China General Microbiological Culture Collection Center, and the strain preservation number is CGMCC No. 23948. The application discloses, for the first time, the degradation effect of Microbacterium sp. Exiguobacteruim sp. ) on triazine herbicide propoxur, and a Microbacterium sp. high-efficiency degrading bacterium SWFU-DH02 is screened, the degradation efficiency of the bacterium on propoxur can reach 75.08%, and the bacterium grows optimally under the conditions of pH 7.0, temperature 28 DEG C and salt concentration 1%. The degrading bacterial strain SWFU-DH02 has the characteristics of simple culture, rapid growth and high efficiency of degradation. The application can solve the pollution problem of triazine herbicide propoxur in soil or water, prevent the phytotoxicity of herbicide residues on subsequent crops, and produce non-toxic and non-polluting green agricultural products. The application provides a new biological approach for modern pesticide pollution control.

Therapeutic compounds and their use in the prevention and treatment of disease are disclosed. The compounds include one or more materials selected from algal biomass / supernatants (including both algae and bacteria), bacterial biomass, and isolated and purified compounds, as well as specific active sites or structures on those compounds. Therapeutic compounds derived from bacteria that appear to selectively alter one or more TLR pathways and the use of such compounds in the prevention and treatment of disease in both animals and humans. The bacteria are selected from the group consisting of Algoriphagus sp., Bosea sp., Brevundimonas sp., Desulfovibrio sp., Microbacterium sp., Sphingomonas sp., and Variovorax sp. The disclosed compounds and methods of treatment find particular use in treating diseases in poultry, particularly in the prevention and treatment of coccidiosis.

The invention relates to a zedoary kwangxi endophyte Microbacterium sp. And application thereof, and belongs to the technical field of agricultural microorganisms. Aiming at the problem that the phosphate-solubilizing function of Microbacterium sp from Guangxi curcuma zedoary is difficult to stably and efficiently express and maintain in the process of preparing a microbial preparation, the invention provides a bacterial strain with the preservation number of GDMCC No: 67431 and a method for preparing the phosphate-solubilizing microbial preparation from the bacterial strain, the method comprises the following steps: activating the bacterial strain to obtain a seed solution; transferring into an inorganic phosphorus liquid fermentation culture medium for shaking culture, centrifugally collecting thalli, finally mixing the thalli with a carrier material containing turf, diatomite and bentonite, and ventilating and drying to obtain the solid phosphorus-dissolving microbial preparation. The strain and the preparation thereof are mainly used for preparing microbial products with the functions of fixing nitrogen, dissolving phosphorus, producing iron carriers and promoting the growth of the curcuma kwangxi.

The invention relates to a microbacterium X10 with a broad-spectrum pesticide degradation function and application of the microbacterium X10, the microbacterium X10 is named as a microbacterium X10 strain, the preservation number of the microbacterium X10 strain is GDMCC No: 66970, and the preservation date of the microbacterium X10 strain is September 17, 2025. The strain has an efficient degradation effect on a plurality of typical pesticides such as dinotefuran, carbendazim, atrazine, pendimethalin and lambda-cyhalothrin, and also has a plurality of probiotic functions of secreting indole-3-acetic acid, dissolving inorganic phosphorus, generating siderophores, antagonizing phytopathogens and the like. The strain can effectively relieve stress of pesticides on plants and promote growth of the plants. The strain can be used for repairing pesticide-polluted soil and promoting plant growth, and has a wide application prospect in green agriculture development.

This invention belongs to the field of microbial technology, specifically relating to a strain of Microbacterium R1 and its applications. The Microbacterium sp. R1 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), with accession number CCTCC M 2024851. This strain exhibits significant control effects against clubroot disease. Verification through examples shows that Microbacterium R1 can inhibit the germination rate of dormant spores of Clubroot by 30.4%. In pot experiments, it can reduce the disease index of clubroot by 48.3%, and in field trials, it can reduce the seedling incidence of clubroot by 30.33% and the disease index by 54.61%. The treated group showed significantly higher rapeseed plant height, growing point height, number of green leaves, total number of leaves, rootstock diameter, fresh weight, effective length of the main inflorescence at the silique stage, and number of effective siliques in the main inflorescence compared to the control group. After treatment with the R1 strain, rapeseed yield increased by 77.53%, and thousand-grain weight increased by 9.4%.

The invention provides a straw degrading bacterium and application of the straw degrading bacterium. The straw degrading bacterium is prepared from microbacterium sp. SDM28 (Microbacterium sp. SDM28) and bacillus (Bacillus sp. SDM29). Through mutual cooperation of microbacilli and bacillus, during straw degradation treatment, compound bacteria can synergistically generate high-activity cellulose degradation related enzymes, lignocellulose structures in straw can be efficiently decomposed, and especially the content of hemicellulose components can be remarkably reduced; the problems that existing straw degrading microorganisms are low in degrading efficiency and difficult to decompose complex lignocellulose structures are solved; meanwhile, the compound bacteria have mild requirements on environmental conditions, the application range focuses on the high-yield straw type of rice straw, large-scale application can be realized without complex regulation and control, resource waste and environmental pollution caused by slow natural decomposition after straw returning to the field are reduced, the resource utilization process of the straw can be promoted, and the economic benefit is increased. The requirements of large-scale and efficient straw treatment can be met.

The invention belongs to the technical field of microorganisms, and particularly relates to a new strain of microbacterium and application thereof. The bacterial strain is separated from rapana venosa, is a new species of Microbacterium sp. M-28, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20241861. The invention also discloses a method for preparing the bacterial strain. The microbacterium sp. M-28 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, shewanella, staphylococcus aureus and proteusbacillus vulgaris, has obvious acetylcholin esterase inhibitory activity, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate casein enzyme and amylase, and can be used for preparing a food additive. The strain can be applied to preparation of a bacteriostatic agent, an acetylcholin esterase inhibitor, an antioxidant, an alpha-glucosidase inhibitor, casein and amylase, can also replace the bacteriostatic agent, the antioxidant, the casein and the amylase in a viable form to be applied to industrial production, and has a very good development and application prospect.

This invention belongs to the field of agricultural microbiology technology, specifically relating to a microbacterium strain and its application in plant disease control. This invention discloses a microbacterium strain screened from wolfberry roots, named... Microbacterium sp. R5-1, deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 34797, has the 16S rDNA sequence shown in SEQ ID NO:1. It secretes siderophores, cellulase, and protease. Experimental results demonstrate that this strain exhibits inhibitory activity against the growth of *Fusarium oxysporum*, *Rhizoctonia solani*, and *Verticillium dahliae*, and shows significant control efficacy against soil-borne fungal diseases of plants caused by these fungi. This invention provides an important microbial resource for the green control of soil-borne fungal diseases of plants.

The invention relates to the technical field of microorganisms, in particular to a microbacterium strain and application thereof in catalyzing 4-HBC to produce 1, 4-HBC. The invention provides a Microbacterium sp. SYP-GBC-2025, which is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No.34395. The invention also provides a preparation method of the Microbacterium sp. The strain can catalyze 4-HBC dehydrogenation to produce 1, 4-HBC, and has the advantages of strong specificity, high conversion efficiency, low production cost, environmental friendliness and the like. Based on the strain, the invention provides a production method of 1, 4-HBC, and the method has the advantages of high efficiency, specificity and low cost, can be used for industrial production of 1, 4-HBC, and provides a key technical support for green synthesis of steroid drug intermediates.

This invention provides a strain of *Exiguobacterium* and its application in the degradation of chloramphenicol and microplastics. The bacterium was collected from a duck farm in Longping Town, Guangdong Province, and obtained through artificial enrichment culture, isolation, and purification. It is named *Exiguobacterium sp. CAP4*. Quantitative analysis using high-performance liquid chromatography (HPLC) and gas chromatography (GC) revealed that the strain *Exiguobacterium sp. CAP4* possesses the ability to efficiently degrade chloramphenicol, mineralize PE microplastics, and various types of plastics. It can mineralize different types of plastics into CO2, indicating that this bacterium has the potential to efficiently degrade chloramphenicol, mineralize PE microplastics, and various types of plastics in environments such as livestock and poultry farms and municipal wastewater, achieving the biodegradation of chloramphenicol, microplastics, and various types of plastics.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a microbacterium strain and application thereof in plant disease control. The invention discloses a microbacterium obtained by screening from a wolfberry root system, the microbacterium is named as Microbacterium sp. R5-1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.34797. The invention further discloses a preparation method of the microbacterium. The 16S rDNA sequence of the strain is as shown in SEQ ID NO: 1, and the strain can secrete and produce siderophores, cellulase and protease. Experimental results prove that the strain has the activity of inhibiting growth of fusarium oxysporum, rhizoctonia solani and verticillium dahliae and has a remarkable control effect on plant soil-borne fungal diseases caused by the fusarium oxysporum, the rhizoctonia solani and the verticillium dahliae. The invention provides an important microbial resource for green prevention and treatment of plant soil fungal diseases.

The invention discloses a xanthan gum main chain lyase mutant with high enzyme activity and a preparation method of the xanthan gum main chain lyase mutant, wild type xanthan gum main chain lyase from Microbacterium sp. XT11 is subjected to site-specific mutagenesis to obtain the xanthan gum main chain lyase mutant with high enzyme activity, and the mutation site of the site-specific mutagenesis comprises at least one of W753V, H755G and L810S. The xanthan gum main chain lyase mutant can directly degrade a xanthan gum main chain under the condition of not carrying out side chain pretreatment, an enzymolysis system is simplified, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 55 DEG C, the improvement amplitude of three mutants W753V / H755G / L810S is maximum, and the enzyme activity of the three mutants W753V / H755G / L810S is remarkably improved. And the limitation that a traditional xanthan gum cracking system depends on branched chain pre-deconstruction is overcome.

The application discloses a high-enzyme-activity xanthan backbone cleavage enzyme mutant and a preparation method thereof. Microbacterium sp. ​ The application discloses a high-enzyme-activity xanthan backbone cleavage enzyme mutant and a preparation method thereof, wherein the wild-type xanthan backbone cleavage enzyme of XT11 is subjected to site-directed mutation, and the mutation point of the site-directed mutation comprises at least one of W753V, H755G and L810S. The xanthan backbone cleavage enzyme mutant of the application can directly degrade the xanthan backbone without side chain pretreatment, thereby simplifying an enzymolysis system, and the enzyme activity of the mutant after being treated by using an alkaline buffer system at 55 DEG C is obviously improved compared with the original enzyme, wherein the three-mutant W753V / H755G / L810S has the largest improvement range, and the three-mutant W753V / H755G / L810S overcomes the limitation that a traditional xanthan cleavage system depends on side chain pre-deconstruction.

The invention provides a new strain of Microbacterium for promoting growth of larvae of hermetia illucens and application of the new strain. The strain comprises a 16S rDNA sequence shown in SEQ ID NO: 1 or a complementary sequence of the 16S rDNA sequence or a sequence with at least 85% of nucleic acid identity compared with the sequence shown in SEQ ID NO: 1. The Microbacterium sp.Micro. M175 disclosed by the invention is separated from insect sand, and has the characteristic of decomposing starch and cellulose. When the strain is added in the breeding process of the hermetia illucens, growth of larvae can be promoted, and accumulation of protein and fat of the larvae is promoted. Compared with sterile water, after the Micr.M175 is added, the body length and body weight of the hermetia illucens larvae are respectively increased by 2.94% and 21.19%, and the accumulation of protein and fat is respectively increased by 32.41% and 47.74%. Therefore, high-quality and high-efficiency breeding of the hermetia illucens is achieved, and industrial production of hermetia illucens bodies is promoted.

The application belongs to the field of microorganisms and particularly relates to a biological nitrogen-fixing bacterium and preparation and application of a microbial inoculum.The biological nitrogen-fixing bacterium is named Microbacterium sp GSS16, classified and named as Microbacterium sp, and preserved in the Guangdong Microbial Culture Collection Center, No. 59 Building, 5th Floor, Guangzhou Xianlie Middle Road 100 Courtyard, on November 14, 2022, with a preservation number of GDMCC No. 62976; and can be used for plant nitrogen fixation, production of nitrogen-fixing enzyme, promotion of plant growth and improvement and fertilization of soil.

The invention discloses a new endophytic growth-promoting microbacterium species F1-18 of lavender and application of the new endophytic growth-promoting microbacterium species The invention provides an endophyte F1-18 separated from a Xinjiang Yili lavender seed. The endophyte F1-18 is a novel microbacterium sp. Strain with a plant growth promoting function, and the endophyte F1-18 is an endophyte F1-18 separated from the Xinjiang Yili lavender seed. The strain F1-18 has no hemolytic activity, has the capabilities of dissolving inorganic phosphorus, producing protease and secreting indoleacetic acid, and has strong tolerance to acid-base and high-salt environments. After the strain F1-18 is treated, the germination rate of lavender and corn seeds can be remarkably increased, the growth and development of lavender, corn and lettuce plants are promoted, the diversity of soil microorganisms is improved, and the microbial community structure is improved. The strain is expected to be developed into a novel agricultural microbial agent, and has important significance in reducing the use of chemical fertilizers, activating soil nutrients, improving the soil quality and improving the plant yield and quality.