Method for producing liquid organic fertilizer by fermentation of poultry feathers with microbes

A technology of liquid organic fertilizer and microbial fermentation, which is applied in the preparation of organic fertilizer, biological organic part treatment, organic fertilizer and other directions, can solve problems such as difficulty in mass production, low secretion of keratinase, limited practical application value of keratinase, etc. Improve environmental quality and solve the effect of feather accumulation

Inactive Publication Date: 2016-11-16
TAISHAN MEDICAL UNIV
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Problems solved by technology

The secretion of keratinase in microbial strains obtained at present is low, and it is diffic

Method used

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Embodiment Construction

[0021] The present invention is further described in detail below.

[0022] A method for producing liquid organic fertilizer by using microorganisms to ferment poultry feathers, characterized in that:

[0023] Feather-degrading bacteria were inoculated in LB medium (Luria-Bertani medium) at 0.5-1% inoculum to activate at 30°C for 24 hours; then inoculated at 2-4% inoculum in LB medium (Luria-Bertani medium). ) in shaking culture to logarithmic phase (OD 600 0.4~0.6, OD: optical density) to obtain the feather-degrading bacterial agent. Cultivate 5M by step-by-step amplification 3 Feather-degrading bacterial inoculant.

[0024] Wet 6,000 kg of chicken or duck feathers with water, add them to a large sterilizer, place them in layers, steam sterilize for 30 minutes, and transfer them to the fermentation tank in the greenhouse. mechanical stirrer, add bacteria-filtered sterile water 120M 3 , join K 2 HPO 4 180 kg, KH 2 PO 4 90kg, NaCl6 5kg, MgSO 4 13kg, add 5M 3 Feathe...

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Abstract

The invention discloses a method for preparing liquid organic fertilizer with an efficient feather degrading bacterium stenotrophomonas maltophilia (Microbacterium sp. TSMC-99). The method is realized by a series of steps: pretreatment of raw materials, preparation of fermenting bacteria of organic fertilizer, optimization of fermenting conditions and production of liquid organic fertilizer. The method makes the most of the poultry feather protein source to produce the liquid organic fertilizer by fermentation, can relieve the insufficiency of organic fertilizer in China, and also can solve the environmental problem caused by a large backlog of feathers. The liquid organic fertilizer produced by microbial fermentative degradation of feather keratin contains rich amino acid and polypeptide and is efficient and environment-friendly green fertilizer; the method has the advantages of mild production conditions, environment friendliness, simple operation, easy popularization and low cost and makes waste profitable; and the technical indexes of the prepared liquid organic fertilizer conform to specifications in the industry standard 'NY525-2012 Organic Fertilizer' of the Department of Agriculture.

Description

technical field [0001] The invention relates to a method for producing liquid organic fertilizer by using microorganisms to ferment poultry feathers. Background technique [0002] Feathers are a major by-product of poultry farming. my country's annual output of feathers is more than 700,000 tons, and with the rapid development of poultry farming, the annual output of feathers is increasing year by year. The crude protein content in poultry feathers is more than 80%, and more than 90% of the feather crude protein is keratin, which is a good source of organic fertilizer. If we can make full use of feather protein sources to produce organic fertilizers, we can alleviate the shortage of high-efficiency organic fertilizer sources in our country, and can also solve the environmental problem of a large backlog of feathers. [0003] Keratin is a fibrous protein with connective tissue and protective functions. It has a stable structure and is rich in disulfide bonds. It is not degr...

Claims

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Application Information

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IPC IPC(8): C05G1/00C05F1/00C05F17/00
CPCC05B7/00C05F1/005C05F17/00C05G5/23C05D5/00C05D9/00C05F11/08Y02A40/20Y02W30/40
Inventor 史仁玖张显忠李艳玲
Owner TAISHAN MEDICAL UNIV
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