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Method for improving crude oil recovery ratio in dolomite reservoir by using microbial flora

A technology of microbial flora and recovery factor, applied in chemical instruments and methods, drilling composition and other directions, can solve problems such as few reports in literature, reduce crude oil viscosity and oil-water interfacial tension, simple operation and low cost Effect

Inactive Publication Date: 2016-05-11
河北地质大学
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] Although many existing patents have reported microbial enhanced oil recovery technology, there are few literature reports on dolomite reservoirs with low porosity, low permeability, and compact cementation.

Method used

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  • Method for improving crude oil recovery ratio in dolomite reservoir by using microbial flora
  • Method for improving crude oil recovery ratio in dolomite reservoir by using microbial flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Weigh Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Microbacterium, Escherichia coli, Pseudomonas putida, Bacillus licheniformis by weight ratio 5: 2: 1: 0.5: 0.5: 0.3: 0.3, Mix well, first inoculate in the culture tank with 2% weight-volume ratio, expand the culture for 12 hours, culture tank rotation speed 150rpm, culture temperature 32°C, control oxygen content 0.1mmol / L during the culture process; then use 5% weight-volume ratio Inoculate in a fermenter, ferment for 24 hours, rotate at a speed of 30 rpm, ferment at a temperature of 32° C., and control the oxygen content to 0.1 mmol / L during the fermenting process to obtain a fermented liquid of the microbial flora. The medium for expanding culture was 5g / L sucrose, 1g / L beef extract, 1g / L peptone, (NH 4 ) 2 SO 4 0.5g / L, MgSO 4 0.3g / L, K 2 HPO 4 0.2g / L, KH 2 PO 4 0.1g / L, CaCl 2 0.1g / L, the balance is water; the fermentation medium is 10g / L sucrose, 5g / L glucose, 3g / L beef extract, 3g / L peptone, ...

Embodiment 2

[0025] Weigh Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Microbacterium, Escherichia coli, Pseudomonas putida, Bacillus licheniformis by weight ratio 15:10:10:8:5:1:1, Mix well, first inoculate in the culture tank with 4% weight-volume ratio, expand the culture for 24 hours, culture tank rotation speed 200rpm, culture temperature 32°C, control oxygen content 0.5mmol / L during the culture process; then inoculate with 8% weight-volume ratio Inoculated in a fermenter, fermented and cultivated for 48 hours, the rotating speed of the fermenter was 80 rpm, the fermentation temperature was 32° C., and the oxygen content was controlled at 0.5 mmol / L during the fermentation process to obtain the fermentation broth of the microbial flora. The culture medium of expansion is 10g / L of sucrose, 3g / L of beef extract, 3g / L of peptone, (NH 4 ) 2 SO 4 0.8g / L, MgSO 4 0.5g / L, K 2 HPO 4 0.4g / L, KH 2 PO 4 0.3g / L, CaCl 2 0.2g / L, the balance is water; the fermentation medium is...

Embodiment 3

[0027] Weigh Pseudomonas aeruginosa, Bacillus cereus, Bacillus subtilis, Microbacterium, Escherichia coli, Pseudomonas putida, Bacillus licheniformis by weight ratio 8:4:3:2:1:0.5:0.5, Mix well, first inoculate in the culture tank with 3% weight-volume ratio, expand the culture for 16 hours, culture tank rotation speed 180rpm, culture temperature 32°C, control oxygen content 0.2mmol / L during the culture process, then inoculate with 6% weight-volume ratio In a fermenter, ferment and cultivate for 36 hours, the rotation speed of the fermenter is 50 rpm, the fermentation temperature is 32° C., and the oxygen content is controlled at 0.2 mmol / L during the fermentation process to obtain the fermentation broth of the microbial flora. The expansion medium was 8g / L sucrose, 2g / L beef extract, 2g / L peptone, (NH 4 ) 2 SO 4 0.6g / L, MgSO 4 0.4g / L, K 2 HPO 4 0.3g / L, KH 2 PO 4 0.2g / L, CaCl 2 0.1g / L, the balance is water; the fermentation medium is 15g / L sucrose, 8g / L glucose, 4g / L b...

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Abstract

The invention relates to a method for improving a crude oil recovery ratio in a dolomite reservoir by using microbial flora, wherein the microbial flora include pseudomonas aeruginosa, bacillus cereus, bacillus subtilis, microbacterium sp., escherichia coli, pseudomonas putida and bacillus licheniformis; the microbial flora are compounded according to the ratio, and through expansion culture and fermentation culture, a fermentation liquid of the microbial flora is prepared; the fermentation liquid is mixed with injected water, a nutrient solution is added into the mixed solution, the mixed solution is injected into a dolomite reservoir core, and after the injected microbial flora act on the dolomite reservoir core, crude oil recovery is performed. The microbial flora can increase the core pore diameter, length and porosity, and lowers the crude oil viscosity and oil-water interfacial tension, so as to improve the crude oil recovery ratio of the dolomite reservoir; moreover, the method has the advantages of simple fermentation process, low cost, simple operation, and high input / output ratio, is suitable for large-scale industrial production, and has good application prospects.

Description

technical field [0001] The invention belongs to the intersecting technical field of geological exploration and microbial oil recovery, and in particular relates to a method for improving oil recovery in dolomite reservoirs by utilizing microbial flora. Background technique [0002] Petroleum is an important energy mineral, which plays an important role in the construction of the national economy and is known as the blood of industry. In the petroleum industry, petroleum is defined as a colored, flammable oily liquid composed mainly of hydrocarbons and having a special odor. Petroleum is mainly formed and preserved in the ground, among which the rock formations capable of producing oil are called source rocks, which are mainly composed of source rocks; the rock formations capable of storing oil are called reservoirs, referred to as reservoirs, which must have storage space (pores property) and the storage space has certain connectivity (permeability). Most of the world's di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K8/582
CPCC09K8/582
Inventor 杜江民张相春钟高润丁强李宁李航申方乐
Owner 河北地质大学
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