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Pseudomonas aeruginosa and applications thereof

A Pseudomonas aeruginosa, a typical technology, applied in the directions of bacteria, microorganisms, drilling compositions, etc., can solve the problem of low biodegradation rate of asphalt, improve the emulsification of crude oil, reduce adhesion, and strong degradation and transformation ability Effect

Active Publication Date: 2017-08-29
陕西博秦生物工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although the biodegradability of asphalt has been proved, compared with saturated hydrocarbons and aromatic hydrocarbons, the biodegradation rate of asphalt is extremely low

Method used

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  • Pseudomonas aeruginosa and applications thereof
  • Pseudomonas aeruginosa and applications thereof
  • Pseudomonas aeruginosa and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Strain screening and identification

[0040] 1. Test method:

[0041] (1) Separation and purification of oil-enhancing bacteria

[0042] Weigh 10.0g of wellhead oil-contaminated soil and add it to 90mL sterile water, and shake it by hand for 15min to obtain 10 -1 Concentration of soil suspension, let it stand for 30s, draw 1mL of soil suspension and add it to 9mL sterile water tube, serially dilute to 10 -3 , the coating method will be 3 concentrations of bacterial suspension into the crude oil filter paper of the separation medium. Cultivate at 37°C for 7 days, pick a single bacterial colony with fast growth and large diameter, and purify it by dilution plate smearing method, and the bacteria that can use crude oil as the only carbon source can be obtained from crude oil-contaminated soil.

[0043] After shaking the crude oil, absorb 0.1mL and spread it evenly on the crude oil filter paper of the separation medium, cultivate and purify in the same way, and then the ...

Embodiment 2

[0057] Application of bacteria

[0058] 1. Test method

[0059] 1.1 Degradation of crude oil

[0060] Add 18.00g of crude oil into a 500mL Erlenmeyer flask, add 200mL of beef extract peptone liquid medium to each bottle, sterilize at 121°C for 30 minutes, inoculate 100mL of bacterial seed solution after cooling, and add 300mL of pure water instead of fermentation broth as a control. Stand at 37°C for 5 days, and shake once every 4 hours during this period. After the treatment, the culture bottle was placed in a refrigerator at 4°C to stand still. After the crude oil solidified, the crude oil and the water phase of the fermentation broth were collected respectively.

[0061] 1.2 Oil displacement characteristics

[0062] Fermentation broth pH: Use the Leici PHS-3D acidity meter to measure the pH value of the aqueous phase of the fermentation broth collected in 1.1.

[0063] Diameter of the oil discharge ring: refers to the size of the oil discharge ring of the liquid culture...

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Abstract

The invention discloses pseudomonas aeruginosa and particularly discloses pseudomonas aeruginosa Gx preserved in CGMCC (China General Microbiological Culture Collection Center) with the preservation number of CGMCC NO. M2016790 on December 30th 2016. The invention also discloses applications of the pseudomonas aeruginosa Gx to asphalt degradation and transformation, colloid degradation and transformation and microbial enhanced oil recovery. The pseudomonas aeruginosa Gx disclosed by the invention has a strong degradation ability on colloid and unknown macromolecule components in asphalt and crude oil, strong surface active material synthesizing ability and acid producing ability and large oil displacing potential in the microbial enhanced oil recovery of high-asphalt content heavy oil reservoirs; and therefore, the pseudomonas aeruginosa Gx is capable of remarkably reducing the adhesion of the crude oil and increasing the gasoline and diesel oil yield in crude oil refining.

Description

technical field [0001] The invention relates to the technical field of microbial enhanced oil displacement and biotransformation, and specifically discloses a strain of Pseudomonas aeruginosa and its application. Background technique [0002] Microbial enhanced oil recovery (MEOR) is one of the most potential oil recovery methods. It has the advantages of high efficiency, low cost, no environmental pollution and convenient operation. It degrades macromolecular hydrocarbons in crude oil and produces biological surfaces. Active species is one of the main mechanisms of MEOR. The microorganisms used in MEOR are mainly bacteria, and Pseudomonas aeruginosa has been paid attention to because of its production of surface active substances. At present, the research on Pseudomonas aeruginosa mainly focuses on the degradation of petroleum pollutants by the bacteria, the properties of the surface active substances produced by the bacteria, the optimization of fermentation conditions fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/385
CPCC09K8/582C12N1/205C12R2001/385
Inventor 高卉薛泉宏来航线
Owner 陕西博秦生物工程有限公司
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