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Copper activation bacterium and method for plants renovation of soil pollution by heavy metal

A heavy metal and bioremediation technology, applied in the field of agriculture and environmental pollution control, can solve the problems of low biomass, difficult preservation and control, secondary pollution of chelating agents, etc., to promote plant growth, improve restoration efficiency, and no secondary pollution. Effect

Inactive Publication Date: 2008-04-02
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there have been successful cases of hyperaccumulative plants for the extraction and remediation of heavy metal-contaminated soils, the current application range is still very limited
The main problems faced by this technology are: (1) most of the hyperaccumulator plants that have been found so far have short plants, low biomass, slow growth, long growth cycle, and only accumulate specific heavy metals, so they are difficult to directly apply; 2) The application of biotechnology to transform hyperaccumulative plants to increase their biomass, or to create new high-biomass plants with higher heavy metal accumulation capabilities has just begun, and it will take a long time to achieve the goal; (3) through chelation Chemical substances such as mixtures, soil improvers, and plant hormones purposely change the mobility of heavy metals in the soil and promote plant growth, but the cost of restoration is high, and residual chelating agents may cause secondary pollution
Due to mycorrhizal problems such as poor stability, difficult storage and control, expensive inoculation, and low efficiency, it is currently difficult to commercialize and scale mycorrhizal

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Insoluble copper carbonate in the activation solution of embodiment 1 bacterial strain JYC17

[0020] Fill 100ml of CuCO into a 250ml Erlenmeyer flask 3 0.100g nitrogen medium (sucrose 10g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 2g, MgSO 4 0.5g, NaCl 0.1g, CaCO 3 0.5g, yeast extract 0.5g), sterilized at 121°C for 30min. Inoculate strain JYC17, shake culture at 30°C and 150r / min on a shaker, take samples at 0, 18, 36, 54, and 72 hours, and measure OD 600 ; Measure the pH value in the culture solution with a pH meter; The culture solution is centrifuged, and the supernatant is taken to measure Cu by atomic absorption method 2+ concentration. The results are shown in Table 1. In the initial growth stage of the bacteria, the effective copper concentration in the culture solution decreases with the increase of the density of the bacteria. In the later stage, the metabolism of the bacteria is the main factor, and the acid production of the bacteria makes the pH of the c...

Embodiment 2

[0021] Precipitated copper hydroxide in soil activated by bacterial strain JYC17 in embodiment 2

[0022] Accurately weigh 10.00 g of a soil sample containing copper hydroxide (Pb 500 mg / kg) and cadmium carbonate (Cd 500 mg. / kg), add it to a 50 ml centrifuge tube, wrap the mouth with gauze, and sterilize under high pressure. Strain JYC17 was cultured on a shaker at 28°C for 20 hours, the bacteria were collected by centrifugation, and the bacterial suspension was made with deionized water. Inoculate 3ml of bacterial suspension into the soil sample, take the same amount of deionized water as the control, set 3 replicates for each treatment, and replenish water regularly. The centrifuge tubes were placed in an incubator at 28°C for 3d, 7d, 13d and 21d and then taken out. Weigh 2g of soil into a 7ml centrifuge tube, add 4ml of water, shake and mix for 2h, centrifuge to take the supernatant to measure the concentration of water-soluble heavy metal Cu. Weigh 1g of soil into a 7ml ...

Embodiment 3

[0023] Embodiment 3 liquid preparation

[0024] The slants were inoculated on a nitrogenous medium (sucrose 10 g, (NH 4 ) 2 SO 4 1g, KH 2 PO 4 2g, MgSO 4 0.5g, NaCl 0.1g, CaCO 3 0.5g, yeast extract 0.5g), cultured for 18 hours and then inserted into the seed tank. The seed tank is 0.5 tons, the feeding amount is 0.4 tons, and the medium composition of the seed tank is 2.0 kg of sucrose, (NH 4 ) 2 SO 4 0.25kg, KH 2 PO 4 0.7kg, MgSO 4 0.17kg, NaCl 0.07kg, CaCO 3 0.4kg, yeast paste 0.2kg. The seed tank must first be sterilized by steam and cooled to 28-30°C, the inoculation volume is 5% by volume, the seed tank culture temperature is controlled at 28-32°C, the stirring speed is 220 rpm, and the sterile air intake is 1:0.8, after 20 hours of cultivation, the seed solution was transferred to the production tank. The production tank is 7 tons, the feeding amount is 4.5 tons, and the medium composition is 8kg of sucrose, 18kg of starch, (NH 4 ) 2 SO 4 4.5kg,...

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Abstract

The invention relates to a copper activated bacterium and a method for strengthening the recovery to the heavy metal pollution in the vegetable soil, and belongs to the agricultural and environmental pollution treatment technical field. The JYC17 culture stain of heavy metal copper activated bacteria is the Microbacterium sp. The effective living microbial numbers of the liquid preparation are above a billion per millimeter, and the effective living microbial numbers of the solid preparation are 200 millions per gram. The using method is that: when the seeds of the plant are seminated in humid soil containing heavy metals, the bioremediation preparation of the JYC17 culture stain is inoculated, and 10 to 20mL of the bacteria liquid of 10<8> bacteria JYC17 / mLs is inoculated in each grams of soil once or in two times. The culture stain has good activation effect to the deposited copper in the solution and the activation rate up to above 73 percent. That the plant is implanted and the bioremediation preparation is inoculated in humid soil containing heavy metals promotes the absorption and the extraction of the plant to the heavy metal copper and improve the extraction and recovery efficiency.

Description

1. Technical field [0001] The invention relates to a copper-activated bacterium and a phytoremediation method for soil heavy metal pollution, and belongs to the technical field of agriculture and environmental pollution control. It specifically involves the use of a bacteria-enhanced plant to extract copper heavy metals in soil, thereby realizing phytoremediation of soil heavy metal pollution. 2. Technical background [0002] Heavy metals are a class of accumulative pollutants that have a wide range of sources in the soil environment and are very harmful. They not only degrade soil fertility, reduce crop yield and quality, deteriorate the water environment, and endanger human life and health through the food chain. Soil heavy metal pollution is becoming more and more serious, therefore, remediation of heavy metal polluted soil has become very urgent. Compared with the traditional physical and chemical methods, which are expensive and complicated to operate, phytoremediation...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10
Inventor 盛下放何琳燕江春玉马海艳
Owner NANJING AGRICULTURAL UNIVERSITY
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