Preparation method of microbacterium for cassava nitrogen fixation

A technology of microbacteria and cassava is applied in the field of preparation of microbacteria for nitrogen fixation in cassava, which can solve the problems of diminishing returns, environmental pollution and the like, and achieve the effect of improving the accumulation of nitrogen.

Inactive Publication Date: 2018-09-28
GUANGXI UNIV
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  • Abstract
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Problems solved by technology

However, the excessive dependence of my country's agriculture on nitrogen fertilizer will inevitably lead to the risk of diminishing returns and environmental pollution, such as soil acidification, salinization, water eutrophication, and global greenhouse effect.

Method used

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  • Preparation method of microbacterium for cassava nitrogen fixation
  • Preparation method of microbacterium for cassava nitrogen fixation
  • Preparation method of microbacterium for cassava nitrogen fixation

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experiment example 2

[0062] The present invention is that the endophytic nitrogen-fixing bacteria A08 strain belongs to Firmicutes (Microbacterium sp.). Deposit unit: General Microorganism Center (CGMCC), China Committee for Microorganism Culture Collection, deposit date: March 07, 2016, deposit number: 12182;

[0063] The biological bacterial agent with microbacteria as the active ingredient in the present invention is a biological bacterial agent prepared from the above bacterial strains through tube seed cultivation and liquid fermentation.

[0064] The preparation method of the biological bacterial agent of the present invention includes tube culture and liquid fermentation experiment methods. Specifically include:

[0065] A: Tube seed culture: Inoculate A08 on the slant medium of the test tube, and culture it at a constant temperature of 28-32°C for 2-3 days to obtain it.

[0066] B: Liquid fermentation: inoculate the test tube seed on the liquid medium, and cultivate it on a shaking table...

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Abstract

The invention discloses a preparation method of microbacterium for cassava nitrogen fixation. The microbacterium belongs to Microbacterium sp.. The microbacterium is collected at the CGMCC on March 07th, 2016 under the number of CGMCC No.12182. A biological agent taking the microbacterium as an active substance is prepared through tube seed culture and liquid fermentation culture. By applying thestrain and the biological agent thereof in the seedling period of cassava growth, nitrogen content of cassava roots, stems and leaves can be increased remarkably, nitrogen accumulation at the early stage of cassava growth is facilitated, and the preparation method has great application value in cassava production.

Description

technical field [0001] This application is a divisional application of "a microbacterium for cassava nitrogen fixation and its preparation method and application". The filing date of the original application is: April 28, 2016, the application number is: 201610274597.8, and the title of the invention is: 一 A microbacterium used for cassava nitrogen fixation and its preparation method and application. The invention belongs to the field of nitrogen-fixing bacteria in the technical field of microorganisms, and in particular relates to a preparation method of a microbacterium used for cassava nitrogen fixation. Background technique [0002] Plant endophytic nitrogen-fixing bacteria are a group of microorganisms that colonize plants and can jointly fix nitrogen with host plants. The use of endogenous nitrogen-fixing bacteria can convert free nitrogen in the atmosphere into nitrogen that plants can directly absorb and use, and then further transform into protein, nucleic acid and o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC05F11/08C12N1/02C12N1/20C12N1/205C12R2001/01
Inventor 何冰张晓顾明华温荣辉
Owner GUANGXI UNIV
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