Preparation method of microbacterium for cassava nitrogen fixation
A technology of microbacteria and cassava is applied in the field of preparation of microbacteria for nitrogen fixation in cassava, which can solve the problems of diminishing returns, environmental pollution and the like, and achieve the effect of improving the accumulation of nitrogen.
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[0062] The present invention is that the endophytic nitrogen-fixing bacteria A08 strain belongs to Firmicutes (Microbacterium sp.). Deposit unit: General Microorganism Center (CGMCC), China Committee for Microorganism Culture Collection, deposit date: March 07, 2016, deposit number: 12182;
[0063] The biological bacterial agent with microbacteria as the active ingredient in the present invention is a biological bacterial agent prepared from the above bacterial strains through tube seed cultivation and liquid fermentation.
[0064] The preparation method of the biological bacterial agent of the present invention includes tube culture and liquid fermentation experiment methods. Specifically include:
[0065] A: Tube seed culture: Inoculate A08 on the slant medium of the test tube, and culture it at a constant temperature of 28-32°C for 2-3 days to obtain it.
[0066] B: Liquid fermentation: inoculate the test tube seed on the liquid medium, and cultivate it on a shaking table...
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