54 results about "Firmicutes" patented technology

The invention discloses a strain of lactobacillus plantarum, which is named as L.plantarum ZW1, and the preservation number of the lactobacillus plantarum is CGMCC (China General Microbiological Culture Collection Center) 26685. The lactobacillus plantarum provided by the invention is a lactic acid bacterium with relatively strong broad-spectrum inhibition capability, and after the interference of organic acid and hydrogen peroxide is eliminated and protease treatment verification is carried out, the strain is determined to have relatively strong bacteriocin production capability. Probiotic performance evaluation shows that the strain has good digestive tract fluid tolerance, and the L.plantarum CGMCC26685 has good intestinal tract probiotic characteristics, can significantly enrich mouse intestinal flora in an in-vitro intestinal flora fermentation model, increase the relative abundance of the phylum of thick-walled bacteria, reduce the relative abundance of the phylum of proteobacteria, proteusbacillus and shigella, and improve the intestinal tract fluid resistance of mice. The number of flora OTU is obviously higher than that of lactobacillus rhamnosus LGG and lactobacillus plantarum L.plantarum PL4 group which does not produce bacteriocin, and the Chao1 index, the Shannon index and the Simpson index are obviously improved compared with those of a control group. It is shown that the L.plantarum CGMCC26685 has good intestinal tract beneficial activity, and has the potential of further performance excavation and application expansion.

The invention belongs to the technical field of microorganisms, and particularly relates to clostridium butyricum and application thereof, and the preservation number of the clostridium butyricum is CGMCC (China General Microbiological Culture Collection Center) No.27853. According to the present invention, with the application of the bacterial strain, the species diversity of the host intestinal flora can be significantly reduced, the Firmicite / Bacteroidetes ratio can be significantly increased, the abundance of harmful bacteria such as Escherichia coli and Salmonella bacteria can be reduced, and the abundance of beneficial bacteria can be improved; in addition, the yield of butyric acid of the clostridium butyricum is larger than or equal to 1.5 g / L, and the activity of amylase in fermentation liquor of the clostridium butyricum is 16.04 + / -0.46 U / mL. When the feed additive is used in feed, intestinal functions of organisms can be effectively improved.

The invention provides a preparation method and application of a biochar-based microbial inoculant, the biochar-based microbial inoculant is prepared from bacillus megatherium L2 with the preservation number of CCTCC NO: M2012381 and rice hull biochar, and the preparation method of the biochar-based microbial inoculant comprises the steps of bacillus megatherium L2 seed liquid culture and liquid fermentation, biochar proportion mixing adsorption, standing and drying. The biochar-based microbial agent prepared by the invention has a good effect of preventing and treating the tea tree anthracnose, effectively reduces the infection ability of tea tree anthracnose related pathogenic bacteria, can also effectively recruit beneficial microorganisms such as chloromentholum, actinomycelum and thick-wall mycolum in soil, further improves the prevention and treatment effect on the tea tree pathogenic bacteria, and has a good application prospect in the field of prevention and treatment of tea tree anthracnose. The method has huge application potential in protection and prevention of the tea trees and is beneficial to good growth of the tea trees.

The present invention provides a compound of Formula (I), which can be used to prepare a composition for losing weight and improving gut microbiota composition. The invention also provides a composition comprising an effective amount of the compound, which has the ability to increase the abundance of Bacteroidales, thereby reducing the ratio of Firmicutes to Bacteroidetes, and can increase the abundance of beneficial gut bacteria and change the gut microbiota composition. The compound and composition of the invention can be applied in food, medicine, feed, nutritional supplements and other uses.

The invention discloses a compound microecological preparation, fermentation liquor and application of the compound microecological preparation in laying hen production. The compound micro-ecological preparation is prepared from clostridium butyricum NIP1021, bacillus cereus 2022B1, bacillus licheniformis 2022B2 and enterococcus faecium 2022B6. The fermentation liquor of the compound micro-ecological preparation can promote the growth performance levels of the Haillan grey laying hens, such as the final weight and the like; the development of thymus and bursa of Fabricius immune organs of laying hens can be promoted; the content of serum immune globulin IgG and serum-related immune cell factors IL-1beta, IL-6 and TNF-alpha of laying hens is remarkably increased, so that the serum nonspecific immune level of animal bodies is improved; according to the method, the intestinal villus area of the laying hens is increased, the nutrient absorption capacity of the laying hens is improved, cecum flora diversity and species abundance are increased, dominant flora categories are thick-wall flora and bacteroides to regulate intestinal flora balance, and the method has positive significance in maintaining intestinal health.

The application discloses a kind of improvement Akkermansia muciniphila The application discloses a starch-based feed for improving bacterial abundance, and belongs to the technical field of functional food. Experimental results show that the starch-based feed can significantly reverse the bacterial flora disorder of mice: PCoA analysis shows that it can restore the overall structure of the disordered bacterial flora to a healthy state; specifically, it improves the ratio of disordered Firmicutes / Bacteroidetes, promotes the proliferation of beneficial bacteria Akkermansia muciniphila, and inhibits the growth of conditional pathogenic bacteria, and the regulatory effect shows a dose-dependent manner. Therefore, the modified starch-based feed is a potential functional food raw material for regulating intestinal flora.

The invention relates to a method for degrading petroleum hydrocarbon in soil by utilizing goethite reinforced endogenous microorganisms, goethite is used as a biological stimulant to activate the activity of soil endogenous functional bacteria for degrading petroleum hydrocarbon, dominant phylum of the soil endogenous functional bacteria is proteobacteria, actinomycetes and thick-wall phylum, and the relative abundance accounts for 50-80%. Under the conditions that the pH value of soil is 6.0-9.0 and the water content of the soil is 20%-50%, the degradation rate of total petroleum hydrocarbon in the soil is larger than or equal to 60%, and the degradation rate of components such as colloid and asphaltene is larger than or equal to 45%.

The invention discloses an inflammatory bowel disease treatment method based on intestinal flora regulatory gene CD24 expression. According to the method, expression or functions of CD24 in host intestinal epithelial cells are up-regulated, the intestinal flora structure is adjusted, and intestinal inflammation is relieved. CD24 up-regulation can be realized by transfecting a CD24 gene overexpression vector, applying a transcription factor or a small molecule agonist or enhancing the activity of an endogenous gene by utilizing a gene editing technology. The CD24 is regulated and controlled, so that the proportion of the phylum firmicalis to the phylum bacteroides can be normalized, the enrichment of short-chain fatty acid producing flora is promoted, the activation of NF-kappa B and NLRP3 inflammasomes is inhibited, and the intestinal barrier function is enhanced. The DSS induced colitis model is established by drinking a 2-5% dextran sodium sulfate solution by a mouse, and simulates the pathological characteristics of human ulcerative colitis. Intervention modes include oral administration, enema or nanoparticle targeted delivery of the CD24 agonist pharmaceutical composition. The invention provides a new strategy for the treatment of inflammatory bowel diseases, especially ulcerative colitis, and has obvious dual curative effects of anti-inflammation and flora regulation.

The main purpose of the present invention is to provide a Monascus pilosus and a composition for reducing weight and improving intestinal flora, the composition comprising the Monascus pilosus and / or a monascus fermentation product thereof, or a functional component comprising the monascus fermentation product. The invention also aims to provide application of the monascus in preparation of the composition for reducing weight and improving intestinal flora. The composition disclosed by the invention has the capabilities of reducing body weight and improving intestinal flora, such as reducing the ratio of the phylum firmicutes to the phylum Bacteroides.

The present invention relates to a use of one or more ratios of quantities of bacteria in the microbiota of a farm animal, in animal feed, said ratio(s) being selected from Firmicutes / Proteobacteria; Clostridium Clusters IV and XIVa / Enterobacteriaceae; Ruminococcaceae and Lachnospiraceae / Enterobacteriaceae; butyrate-producing bacteria / Firmicutes; aerobic bacteria / anaerobic bacteria. It / They may be used as marker(s) to determine the status of the intestinal microbiota of a farm animal or for selecting an extract of a satisfactory intestinal microbiota of a farm animal, intended to maintain or to improve the intestinal microbiota of a different farm animal in need thereof.

The present invention belongs to the field of medical technology, and specifically relates to the use of Mishitong capsule in the preparation of drugs for intestinal flora disorder caused by kidney stones; the present invention discusses the diversity, richness and uniformity of intestinal flora through Alpha diversity analysis, hierarchical clustering analysis, PCoA analysis, community column chart (phylum and genus level) analysis, species Venn diagram analysis, heat map analysis, PCA analysis, etc. The results show that Mishitong capsule can regulate the intestinal flora of mice by increasing the proportion of Firmicutes and Proteobacteria, effectively improve the diversity, richness and uniformity of intestinal flora, improve the structure of intestinal flora, and maintain the balance of intestinal flora. The discovery of this new use provides a new method and favorable basis for the treatment of intestinal flora disorder.

The present invention provides a preparation method and application of a biochar-based microbial inoculant. The biochar-based microbial inoculant is made from Bacillus megaterium L2 with the preservation number of CCTCC NO: M2012381 and rice husk biochar. The preparation method includes the cultivation of Bacillus megaterium L2 seed liquid and liquid fermentation, the proportion mixing adsorption and static settling of biochar, and drying. The biochar-based microbial inoculant prepared by the present invention has a good effect on the prevention and control of tea tree anthracnose, effectively reduces the infection ability of tea tree anthracnose-related pathogenic bacteria, and can also effectively recruit beneficial microorganisms such as Chloroflexi, Actinobacteria and Firmicutes in the soil, further improving the prevention and control effect on tea tree pathogenic bacteria. It has great application potential in the protection and prevention of tea trees and is beneficial to the good growth of tea trees.

To provide a method and device that can maintain indoor microbial diversity in a simple manner.SOLUTION: Provided is a method for maintaining indoor microbial diversity, comprising spraying, indoors, a liquid composition containing Firmicutes bacteria that are nonpathogenic to humans and animals. Also provided is a method for maintaining indoor microbial diversity, comprising spraying, after spraying a disinfecting composition or a disinfecting gas, a liquid composition containing Firmicutes bacteria that are nonpathogenic to humans and animals.SELECTED DRAWING: Figure 1

The invention relates to the technical field of nano materials, in particular to a multifunctional electrospun nanofiber with bacteriostatic and anti-tumor effects and a preparation method and application of the multifunctional electrospun nanofiber. The result of the specific embodiment of the invention proves that the polyethylene oxide / gellan gum-carvacrol electrospun nanofiber can prolong the shelf life of strawberries by 3-4 days, and effectively alleviates the weight loss ratio, hardness, acidity, color and other quality deterioration of strawberry fruits. Microbial colony structure analysis shows that PEO / GG-CAR treatment can effectively reduce the flora richness and species number of strawberries, inhibit related putrefying bacteria such as proteobacteria, and promote the relative abundance of anaerobic microorganisms such as firmicobacteria and the like to be improved at the same time. Principal component analysis proves that the electrospun nanofiber membrane added with the CAR can effectively regulate microbial communities of strawberries, so that the preservative and fresh-keeping effects are achieved. Meanwhile, the electrospun nanofiber membrane also has the effect of inhibiting cancer cells.

The invention relates to the technical field of medicine, and particularly discloses a liver cirrhosis specific intestinal flora marker composition and application of the liver cirrhosis specific intestinal flora marker composition in noninvasive diagnosis. The intestinal microbial flora is selected from the group consisting of clostridium, Bacteroides, Thalictrum, Wehonor genus, Streptococcus genus, Klebsiella genus, and Prevotella genus; according to the method, a non-invasive solution is provided for screening and diagnosis of liver cirrhosis by detecting a synergistic change mode of specific florae such as clostridium, bacteroides, thickenella, prevotella, Wehonor, streptococcus and klebsiella in an excrement sample; the limitation that traditional liver needle biopsy is invasive and imaging examination is insensitive to early-stage lesions is avoided.

The application discloses a microorganism marker combination for identifying nest susceptibility of chickens and application thereof, and belongs to the technical field of poultry breeding and microbiomics. The microorganism marker combination comprises relative abundance information of at least two bacterial phyla in Bacteroidetes, Firmicutes and Verrucomicrobia in cecal contents. When at least two of the following conditions are met: the abundance of Bacteroidetes is higher than 35%, the abundance of Firmicutes is lower than 35%, and the abundance of Verrucomicrobia is lower than 2%, it is determined that the chicken has nest susceptibility or is in a nest period. Based on the marker combination, the application provides a corresponding identification method, a regulation method and a regulation agent screening strategy.

The invention relates to the technical field of livestock breeding and animal nutrition, in particular to fattening pig feed and application thereof.The fattening pig feed is prepared from, by weight, 50 parts of corn flour, 19-21 parts of concentrated feed, 9-11 parts of wheat bran and 20 parts of wild buckwheat straw powder. According to the formula, the wild buckwheat straw is used as a functional fiber source, through scientific matching and synergistic effect with conventional energy raw materials, the micro-ecological structure of the intestinal tract of the fattening pig is effectively optimized, enrichment of beneficial microbial communities such as lactobacilli in the phylum firmicalis is promoted, and therefore the steady state of the intestinal tract can be maintained. Meanwhile, in the liver of the fattening pigs fed with the formula, expression of various genes related to extracellular matrix structures and tissue homeostasis is up-regulated, and support is provided for tissue structure maintenance and healthy growth. The method provides a feasible path for resource utilization of wild buckwheat straws, and has double benefits of breeding health promotion and agricultural byproduct value increase.

The main objective of the present invention is to provide a lactic acid bacterial strain and a composition for improving gut microbiota composition. The composition comprises the lactic acid bacterial strain and / or extracellular vesicles secreted by the lactic acid bacterial strain. Another objective of the present invention is to provide a method for improving gut microbiota composition and products of the lactic acid bacterial strain. Additionally, the composition of the present invention has the capability to influence the growth of Firmicutes and Bacteroidetes, thereby leading to an improvement in gut microbiota composition.

The present invention discloses use of Streptococcus thermophilus ST7 for modulating immunity, comprising using the Streptococcus thermophilus ST7 as an active ingredient for modulating immunity, wherein the Streptococcus thermophilus ST7 are deposited under the accession Nos. BCRC911126 and DSM34255. By administering an effective dose of Streptococcus thermophilus ST7 to an individual, the expression level of IL-12p40 on cells can be increased, the gut microbiota can be modulated and the Firmicutes / Bacteroidetes ratio in the intestine increases to improve immunity and antiviral ability.

The invention discloses an application of premna microphylla pectin in preparation of a product for preventing and improving diet-induced obesity. The premna pubescens pectin is prepared from premna pubescens leaves as a raw material through extraction, purification and other processes. According to the present invention, the research on the high fat diet induced obese mouse model finds that the body lipid metabolism is regulated by remodeling the intestinal flora structure, particularly reducing the ratio of the phylum firmicutes to the phylum bacteroides, and specifically enriching the beneficial flora such as Muribacaceae and the like by using the premna microphylla pectin; experimental results prove that the premna microphylla pectin can significantly inhibit pathological hypertrophy of adipocytes, reduce visceral fat accumulation, effectively control weight gain and improve dyslipidemia and liver fatty degeneration, and has good biological safety.

Dysbiosis has been linked to diseases such as inflammatory bowel disease and obesity. Multi-omics studies have uncovered significant associations between microbiota genes and diseases. Many of these genes are exclusively expressed in non-model microbes such as Firmicutes / Clostridia. A pipeline for building microbial genetic manipulation systems would be a first step to manipulating these genes in vivo and causally connecting them with host diseases. The present technology relates generally to compositions and the methods of preparations thereof for genetically engineering gut-microbiota in vitro. The present technology further relates to uses of compositions in vivo.

The invention discloses a method for regulating and controlling propagation of populus euphratica and stability of rhizosphere microorganisms through branched seepage rotation irrigation, and relates to the technical field of ecological restoration. According to the method, by differentiating branched seepage rotation irrigation modes, precisely matching habitat requirements of sexual and clone propagation populus euphratica seedlings, and adopting a long-interval and gradually-water-increasing irrigation mode for the clone propagation seedlings, the propagation of populus euphratica and the stability of rhizosphere microorganisms are regulated and controlled; according to the method, soil salt leaching and nitrogen and phosphorus accumulation can be promoted, functional florae such as rhizosphere actinomycetes and thick-wall bacteria are activated, complex organic matter is degraded by secreting extracellular enzyme, the phosphorus dissolving capacity is enhanced, the nutrient obtaining efficiency of seedlings in a medium-high-salt environment is improved, a short-interval and high-water-quantity mode is adopted for sexual propagation seedlings, a low-salt and high-humidity habitat is created, and the survival rate of the seedlings is increased. Growth-promoting florae such as pseudomonas are enriched, and by secreting growth hormones and antagonistic substances, the planting capacity of the seedlings in a humid environment is enhanced, so that directional regulation and control of the two breeding modes are realized.

The invention belongs to the technical field of intestinal flora transplantation, and discloses an intestinal flora capsule for precise intestinal flora transplantation and a preparation method and application thereof. The invention provides application of a composition comprising maltodextrin and a cellulose ether derivative in preparation of an intestinal flora colonization accelerant. In the composition, due to the synergistic effect of the maltodextrin and the cellulose ether derivative, not only can intestinal flora be effectively protected from ultralow-temperature damage, but also the cell structure, functionality, cell metabolic activity and microenvironment adaptability required by colonization of the firmicite bacteria and the Bacteroides bacteria can be optimized, and the composition can be used for preparing the medicine for treating the intestinal tract diseases and the intestinal tract diseases. The freeze-drying protective agent has the effect of improving the colonization rate of the phylum firmicalis bacteria and the phylum bacteroides bacteria, can be used as a freeze-drying protective agent and a colonization accelerant of the intestinal flora at the same time, and has very excellent application prospects in preparation of products such as intestinal flora capsules and the like.

The current invention relates to relates to methods of improving commensals in an animal by feeding the animal with a diet including quinoa grain. The quinoa grain is in an amount effective to increase parameters for commensals such as the percentage of Lactobacillus in total microbiota, the percentage of bifidobacteria in total microbiota, the percentage of Clostridium in total microbiota, and the firmicutes to bacteroidetes ratio. The current invention also relates to pet food compositions that include effective amount of quinoa grain to increase the commensals parameters. In addition, the methods of making such a food compositions are also disclosed.

The application discloses Slackia a new application of sp.D-G6, and particularly relates to Slackia application of sp.D-G6 bacterial powder in pig breeding, and belongs to the field of agricultural animal breeding and microbial technology. The bacterial strain has been preserved in the Guangdong Microbial Culture Collection Center on May 8, 2019, and the preservation number is GDMCC NO: 60661. Research shows that the bacterial powder has no obvious adverse effect on pigs, has good feeding safety, can promote pig feeding and weight gain, improve average daily feed intake and average daily weight gain, and improve growth performance. Further, the bacterial powder can regulate the microbial composition of the ileum of growing pigs, increase the abundance of Firmicutes and Lactobacillus, Lactobacillus and reduce the abundance of Proteobacteria and / or Escherichia-Shigella, Escherichia-Shigella and has application prospect of being developed into a new green feed additive.

The present invention relates to a brown rice fermentation product and its uses, more specifically, to the use of the brown rice fermentation product to moisten the intestines, improve bowel movements, and contribute to the regulation of the intestinal microbiota. The present invention has found that the brown rice fermentation product can improve constipation, have NO removal effects, and down-regulate the relative expression levels of the nos1, nos2a, and nos2b genes. Treatment with the brown rice fermentation product can regulate the species richness of the intestinal microbiota of constipated zebrafish, with certain effects on species uniformity and diversity, increasing the abundance of beneficial bacteria (Bacteroidetes) and decreasing the abundance of harmful bacteria (Firmicutes, Aeromonas, Enterobacteriaceae). The brown rice fermentation product of the present invention can be widely used in the food industry as a functional ingredient.

The invention discloses an application of patchouli alcohol as a flora regulator. According to the application of patchouli alcohol provided by the invention in preparation of medicines, foods, drinks or health-care products for promoting growth and / or proliferation of bacteria, the bacteria are selected from one or two of bacteria of the phylum firmicules and bacteria of the phylum actinobacteria, and the application of patchouli alcohol in preparation of medicines, foods, drinks or health-care products is also provided, wherein the bacteria are selected from one or two of bacteria of the phylum firmicules and bacteria of the phylum actinobacteria. The patchouli alcohol performs targeted regulation on intestinal flora by improving the abundance of intestinal probiotics and reducing the abundance of harmful bacteria, and provides a new thought and direction for development of drugs for targeted regulation of the intestinal flora in future.

The invention discloses a pseudo-ginseng active composition, meal replacement powder and application of the pseudo-ginseng active composition and the meal replacement powder in improving intestinal microecology, losing weight, reducing blood sugar and reducing lipid. The panax notoginseng active composition comprises 15 active components and not less than 1 billion / g of bacillus inulin viable bacteria powder, the inhibition rate on alpha-glucosidase is not less than 51.49%, and the OARC value is not less than 32.60 micromoles TE / mL. The meal replacement powder prepared from the pseudo-ginseng active composition not only has the effects of reducing blood sugar and blood fat and losing weight on diabetic model mice, but also has small damage to mouse visceral organs, and the analysis of mouse intestinal flora finds that the meal replacement powder provided by the embodiment of the invention has the effect of improving the abundance of mouse intestinal thick-wall mycophylum; the intestinal microecology of the mouse can be favorably converted to the probiotic direction.

The present invention relates to the use of one or more ratios of the amount of bacteria in the microbiota of a farm animal as a marker to determine the status of the intestinal microbiota of a farm animal and whether this status is satisfactory, or to the use of an extract for selecting a satisfactory intestinal microbiota of a farm animal, intended to preserve or improve the gut microbiota of different farm animals in need thereof, said ratio being selected from the group consisting of Phylum Thallungiae / Phylum Proteinae; clostridium IV and XlVa cluster / Enterobacteriaceae; a ruminaceae family and a hispiraceae / enterobacteriaceae family; butyrate-producing bacteria / phylum of Thalictrum; aerobic bacteria / anaerobic bacteria.

The invention provides a composite complete set of health-care product composition based on double extraction of gold and radix rehmanniae as well as a preparation method and application of the composite complete set of health-care product composition. The complete set of health-care product composition is constructed based on yin deficiency and internal heat and circadian rhythm regulation and control concepts of traditional Chinese medicine. The beverage set contains three kinds of a honeysuckle probiotic composite beverage, a Jinzhi double-extraction fermentation beverage and spina date seed composite particles. Through systematic compatibility, a specific'golden-land symbiotic fermentation 'process and a systematic time-sharing intervention scheme based on circadian rhythm, cell and animal experiments prove that the product can significantly inhibit release of inflammatory factors, improve the activity of antioxidant enzymes, relieve liver cell lipid accumulation and endoplasmic reticulum stress, improve the immunity of liver cells and improve the immunity of the liver cells. The traditional Chinese medicine composition is capable of improving anxiety-like behaviors and sleep structures, regulating intestinal flora structures, increasing abundance of beneficial bacteria such as phylum firmicalis and reducing abundance of harmful bacteria such as proteobacteria, so that an overall conditioning effect is achieved at multiple targets, and a systematic intervention scheme based on circadian rhythm is provided for improving the sub-health status of modern pressure people.