Intestinal flora capsule for precise intestinal flora transplantation as well as preparation method and application of intestinal flora capsule

Abstract

The invention belongs to the technical field of intestinal flora transplantation, and discloses an intestinal flora capsule for precise intestinal flora transplantation and a preparation method and application thereof. The invention provides application of a composition comprising maltodextrin and a cellulose ether derivative in preparation of an intestinal flora colonization accelerant. In the composition, due to the synergistic effect of the maltodextrin and the cellulose ether derivative, not only can intestinal flora be effectively protected from ultralow-temperature damage, but also the cell structure, functionality, cell metabolic activity and microenvironment adaptability required by colonization of the firmicite bacteria and the Bacteroides bacteria can be optimized, and the composition can be used for preparing the medicine for treating the intestinal tract diseases and the intestinal tract diseases. The freeze-drying protective agent has the effect of improving the colonization rate of the phylum firmicalis bacteria and the phylum bacteroides bacteria, can be used as a freeze-drying protective agent and a colonization accelerant of the intestinal flora at the same time, and has very excellent application prospects in preparation of products such as intestinal flora capsules and the like.

Description

Technical Field

[0001] This invention belongs to the technical field of intestinal flora transplantation, and particularly relates to an intestinal flora capsule for precise intestinal flora transplantation, its preparation method, and its application. Background Technology

[0002] Intestinal flora capsules refer to a class of preparations that supplement intestinal flora orally to regulate the balance of the intestinal microecology. Their core function is to rebuild or strengthen beneficial intestinal flora, resulting in improved digestive function, enhanced immunity, and prevention of related diseases. Intestinal flora capsules are generally prepared by filling capsules with liquid live bacteria preparations; that is, they contain un-lyophilized liquid live bacteria preparations. To extend the shelf life and improve the stability of intestinal flora capsules, they need to be stored at -80°C. However, the ultra-low temperature of -80°C can also damage the intestinal flora. The ice crystals formed by the water in the liquid live bacteria preparation during the cooling process can pierce the cell walls and cell membranes of bacteria, leading to bacterial death.

[0003] Currently, trehalose, sucrose, skim milk powder, and other protein- or polysaccharide-based preservatives are added to liquid live bacteria preparations as protective agents. These agents reduce ice crystal formation through hydration, effectively minimizing bacterial death during ultra-low temperature refrigeration and protecting the intestinal flora. However, the introduction of these protective agents may alter bacterial membrane structure and function, reducing the binding and adhesion of cells to intestinal tissues. Furthermore, while the protective film formed on the cell surface can effectively reduce ultra-low temperature damage, additional energy is required to degrade and destroy this film during colonization, preventing cells from rapidly initiating metabolism. These protective agents are particularly problematic for Firmicutes (…). Firmicutes ) and Bacteroidetes ( Bacteroidetes Firmicutes (Bacteria) have a more significant inhibitory effect on the colonization process of bacteria in the intestine. Firmicutes ) and Bacteroidetes ( Bacteroidetes The colonization rate of bacteria decreased by 30% to 60%, which led to the failure to rebuild the balance of the intestinal microecology and the aggravation of intestinal flora imbalance, thus having significant limitations. Summary of the Invention

[0004] The primary objective of this invention is to address the problem that existing protective agents applied to intestinal flora capsules cause problems with Firmicutes (Bacteroides spp.). Firmicutes ) and Bacteroidetes ( Bacteroidetes This addresses the problem of unsatisfactory bacterial colonization rates and provides the application of compositions including maltodextrin and cellulose ether derivatives in the preparation of intestinal flora colonization promoters.

[0005] The second objective of this invention is to provide an intestinal flora capsule.

[0006] A third objective of this invention is to provide a method for preparing the above-mentioned intestinal flora capsules.

[0007] A fourth objective of this invention is to provide the application of the above-mentioned intestinal flora capsules in the preparation of drugs for flora transplantation.

[0008] Specifically, the present invention provides the application of the composition in the preparation of an intestinal flora colonization promoter, the composition comprising maltodextrin and cellulose ether derivatives in a mass ratio of (10~40):(0.5~5).

[0009] Furthermore, the composition promotes Firmicutes ( Firmicutes Bacteria and / or Bacteroidetes ( Bacteroidetes Bacteria colonize the gut.

[0010] Furthermore, the cellulose ether derivative is selected from one or more of hydroxypropyl methylcellulose, hydroxypropyl cellulose, and hydroxyethyl cellulose.

[0011] Furthermore, the viscosity of the cellulose ether derivative is 40cp~60cp.

[0012] Furthermore, the DE value of the maltodextrin is 1% to 25%.

[0013] The intestinal flora capsule provided by the present invention comprises a mixed bacterial slurry, which is composed of maltodextrin, cellulose ether derivatives and intestinal flora slurry added in a mass ratio of (10~40):(0.5~5):(10~100).

[0014] Furthermore, the cellulose ether derivative is selected from one or more of hydroxypropyl methylcellulose, hydroxypropyl cellulose, and hydroxyethyl cellulose.

[0015] Furthermore, the viscosity of the cellulose ether derivative is 40cp~60cp.

[0016] Furthermore, the cellulose ether derivative is hydroxypropyl methylcellulose.

[0017] Furthermore, the hydroxypropyl cellulose has a methoxy content of 10% to 30%, a hydroxypropoxy content of 1% to 15%, and a viscosity of 40 to 60 cp.

[0018] Furthermore, the DE value of the maltodextrin is 1% to 25%.

[0019] Furthermore, in the mixed bacterial sludge, the concentration of maltodextrin is 10wt%~40wt%, the concentration of cellulose ether derivatives is 0.5wt%~5wt%, and the concentration of intestinal flora sludge is 55wt%~89.5wt%.

[0020] The preparation method of the intestinal flora capsule provided by the present invention includes: mixing maltodextrin, cellulose ether derivatives and intestinal flora slurry evenly to obtain mixed slurry; filling the mixed slurry into a capsule shell to obtain the intestinal flora capsule.

[0021] The present invention also provides the application of the above-mentioned intestinal flora capsules in the preparation of drugs for flora transplantation.

[0022] Beneficial effects: In the composition provided by this invention, the synergistic effect between maltodextrin and cellulose ether derivatives added in a specific mass ratio not only effectively protects the intestinal flora from ultra-low temperature damage, but also optimizes the Firmicutes phylum (…). Firmicutes Bacteria and Bacteroidetes ( Bacteroidetes The cell structure and functionality required for bacterial colonization, cell metabolic activity, and adaptation to the microenvironment have the effect of improving the colonization rate of Firmicutes and Bacteroidetes. It can be used as both a freeze-drying protectant and a colonization promoter for intestinal flora, and has excellent application prospects in the preparation of intestinal flora capsules and other products. Detailed Implementation

[0023] The inventors of this invention applied existing cryoprotectants such as trehalose, sucrose, and skim milk powder to intestinal flora capsules, specifically targeting the Firmicutes phylum (…). Firmicutes ), Bacteroidetes ( Bacteroidetes To address the issues of unsatisfactory bacterial colonization rates and protective effects, after extensive and in-depth consideration and numerous studies and experiments, a creative discovery was made: Adding cellulose ether derivatives such as hydroxypropyl methylcellulose (generally used as excipients) to maltodextrin in intestinal sludge can effectively form a protective film on the surface of intestinal flora cells or cell clusters, reducing damage to the intestinal flora during ultra-low temperature refrigeration and exhibiting excellent cryogenic protection. Simultaneously, Firmicutes and Bacteroides bacteria in the intestinal environment can synergistically degrade cellulose ether derivatives, and this synergistic effect can be effectively enhanced by maltodextrin. Firmicutes and Bacteroides bacteria require less time to destroy the protective film formed by cellulose ether derivatives and maltodextrin, rapidly initiating metabolism and entering the colonization window, thus giving them a superior competitive advantage in colonization and effectively improving their colonization rate in the intestinal environment. Based on this, the technical solution of this invention was obtained.

[0024] This invention provides the use of a composition in the preparation of an intestinal flora colonization promoter. Specifically, the composition consists of maltodextrin and cellulose ether derivatives.

[0025] In this invention, maltodextrin refers to a type of polysaccharide produced by hydrolyzing starch, and is an incomplete hydrolysis product of starch.

[0026] In some specific embodiments, the DE value of the maltodextrin is preferably 1% to 25%, specifically 1%, 1.5%, 2%, 5%, 8%, 10%, 12.5%, 15%, 18%, 20%, 23%, 25%, or any value between them; more preferably 15% to 20%. In this case, the maltodextrin can better promote the synergistic metabolism of cellulose ether derivatives by Firmicutes and Bacteroidetes bacteria, thereby achieving better colonization of Firmicutes and Bacteroidetes bacteria in the intestine.

[0027] According to GB / T 20882.6-2021 Starch Sugar Quality Requirements Part 6: Maltodextrin, the DE value is defined as the glucose equivalent value, which is the mass fraction of reducing sugar in the dry matter of maltodextrin, in units of 0.5%.

[0028] In this invention, the cellulose ether derivatives refer to a class of polysaccharide derivatives obtained by modifying natural cellulose through a chemical etherification reaction. Specific examples include, but are not limited to, one or more of hydroxypropyl methylcellulose, hydroxypropyl cellulose, and hydroxyethyl cellulose.

[0029] In some specific embodiments, the viscosity of the cellulose ether derivative is preferably 40 cp to 60 cp, specifically 40 cp, 42 cp, 44 cp, 45 cp, 47.5 cp, 50 cp, 51.2 cp, 53 cp, 55 cp, 58 cp, 60 cp, or any value between them; more preferably 48 cp to 55 cp. In this case, the length of the cellulose ether derivative is suitable, which can shorten the time required for cells to rapidly initiate metabolism and enter the colonization window while maintaining the metabolic advantages of Firmicutes and Bacteroides bacteria compared to other bacterial groups, thereby achieving better colonization of Firmicutes and Bacteroides bacteria in the intestine.

[0030] In this invention, the cellulose ether derivative is preferably hydroxypropyl methylcellulose. More specifically, the methoxy group content of the hydroxypropyl cellulose is preferably 10% to 30%, specifically 10%, 12.5%, 13%, 15%, 18%, 20%, 25%, 28.2%, 30%, or any value between them; more preferably 23% to 30%. The hydroxypropoxy group content of the hydroxypropyl cellulose is preferably 1% to 15%, specifically 1%, 2%, 2.5%, 3%, 4.8%, 5%, 6%, 8.1%, 10%, 12.5%, 15%, or any value between them; more preferably 5% to 10%. The viscosity of the hydroxypropyl cellulose is preferably 40 cp to 60 cp, specifically 40 cp, 42.5 cp, 45 cp, 48 cp, 50 cp, 51.2 cp, 55 cp, 58 cp, 60 cp, or any value between them; more preferably 48 cp to 55 cp. At this point, the introduction of hydroxypropyl methylcellulose has a better synergistic effect with maltodextrin, which can achieve better colonization of Firmicutes and Bacteroidetes in the intestine.

[0031] In this invention, the preferred mass ratio of the added maltodextrin and cellulose ether derivative in the composition is (10~40):(0.5~5), specifically it can be 10:0.5, 10:1, 20:1, 25:1, 27.5:1, 30:1, 40:3, 40:5 or any value between them.

[0032] Based on the application potential of maltodextrin and cellulose ether derivatives in the preparation of intestinal flora colonization promoters, this invention also provides an intestinal flora capsule. The intestinal flora capsule comprises a mixed bacterial slurry, which is composed of maltodextrin, cellulose ether derivatives, and intestinal flora slurry. The specific mass ratio of the added maltodextrin, cellulose ether derivatives, and intestinal flora slurry is (10~40):(0.5~5):(10~100), specifically 10:0.5:10, 10:1:10, 20:2:15, 30:5:49, 40:5:100, or any value between them.

[0033] In this invention, the cellulose ether derivatives and maltodextrin in the mixed bacterial sludge can be completely or partially the same as the aforementioned cellulose ether derivatives and maltodextrin, which will not be elaborated here.

[0034] In this invention, the intestinal flora sludge refers to a semi-solid or paste-like preparation rich in intestinal flora obtained by using samples from healthy donors as raw materials and purifying them through steps such as filtration and centrifugation. The method for preparing intestinal flora sludge is a conventional technique used in existing microbiota transplantation technology, and this invention does not impose any particular limitations on it.

[0035] In this invention, the concentration of maltodextrin in the mixed bacterial sludge is preferably 10wt% to 40wt%, specifically 10wt%, 11.5wt%, 12wt%, 13wt%, 15wt%, 20wt%, 25wt%, 30wt%, 40wt%, or any value between them; the concentration of the cellulose ether derivative is preferably 0.5wt% to 5wt%, specifically 0.5wt%, 0.8wt%, 1wt%, 1.5wt%, 2wt%, 3wt%, 4wt%, 5wt%, or any value between them; the concentration of the intestinal flora sludge is preferably 55wt% to 89.5wt%, specifically 55wt%, 58wt%, 60wt%, 62.5wt%, 75wt%, 80wt%, 85.5wt%, 89.5wt%, or any value between them.

[0036] In this invention, the intestinal flora capsule specifically includes a capsule shell for containing the mixed bacterial sludge. The capsule shell is a conventionally used technique in existing flora transplantation technology, and this invention does not impose any particular limitations on it.

[0037] Based on obtaining the above-mentioned intestinal flora capsules, the present invention also provides a method for preparing intestinal flora capsules. The preparation method specifically includes: mixing maltodextrin, cellulose ether derivatives, and intestinal flora slurry evenly to obtain a mixed slurry; filling the mixed slurry into capsule shells to obtain the intestinal flora capsules.

[0038] In this invention, the method of uniformly mixing maltodextrin, cellulose ether derivatives and intestinal flora sludge is a conventional technique used in the prior art, limited to achieving sufficient mixing and dispersion of each component, and this invention does not impose any particular limitations on it.

[0039] In some specific embodiments, the preferred method for uniformly mixing the maltodextrin, cellulose ether derivatives, and intestinal flora slurry includes: uniformly mixing the maltodextrin and intestinal flora slurry, followed by adding the cellulose ether derivative and mixing uniformly to obtain the mixed slurry. This mixing method allows for better formation of a protective film primarily composed of maltodextrin and cellulose ether derivatives within the intestinal flora cells or cell clusters, thereby achieving superior cryoprotection and colonization promotion effects.

[0040] Based on the excellent low-temperature storage stability and colonization effect of the above-mentioned intestinal flora capsules on Firmicutes and Bacteroidetes, the present invention also provides the application of the above-mentioned intestinal flora capsules in the preparation of drugs for flora transplantation.

[0041] The embodiments of the present invention are described in detail below. These embodiments are intended to explain the present invention and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature in the art or according to the product instructions. Reagents or instruments used, unless otherwise specified, are all commercially available conventional products.

[0042] It should be noted that the intestinal microbiota sludge used in the following examples all came from the same donor feces preserved at the same time, and the preparation methods, reagents, and conditions used were all consistent. The preparation of the intestinal microbiota sludge specifically included: purifying the donor feces using an automated fecal microbiota extractor (purchased from Xiamen Chengge Biotechnology Co., Ltd.) according to the instruction manual to obtain the intestinal microbiota sludge.

[0043] Example 1 This embodiment illustrates the preparation of an intestinal flora capsule, specifically including: mixing maltodextrin (Weifang Shengtai Pharmaceutical Co., Ltd., DE value 19.8%) with intestinal flora sludge according to the concentrations shown in Table 1, then adding hydroxypropyl methylcellulose (Shandong Guangda Technology Development Co., Ltd., methoxy content 28.2%, hydroxypropoxy content 8.1%, viscosity 51.2cp) and mixing evenly to obtain a mixed sludge; filling the mixed sludge into enteric-coated gelatin empty capsules (purchased from Anhui Huangshan Capsule Co., Ltd.) at a filling volume of 0.7g / capsule to obtain intestinal flora capsules.

[0044] Table 1.

[0045] Example 2 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of maltodextrin is 10 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0046] Example 3 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of maltodextrin is 30 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0047] Example 4 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of maltodextrin is 40 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0048] Example 5 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of hydroxypropyl methylcellulose is 0.5 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0049] Example 6 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of hydroxypropyl methylcellulose is 1.0 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0050] Example 7 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that the final concentration of hydroxypropyl methylcellulose is 5.0 wt%, while other conditions remain the same, to obtain intestinal flora capsules.

[0051] Example 8 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that hydroxypropyl methylcellulose (Shandong Guangda Technology Development Co., Ltd., methoxy content 28.6%, hydroxypropoxy content 8.3%, viscosity 3990cp) with the same added concentration is used instead of hydroxypropyl methylcellulose (Tai'an Ruitai Cellulose Co., Ltd., methoxy content 28.2%, hydroxypropoxy content 8.1%, viscosity 51.2cp). All other conditions are kept the same to obtain intestinal flora capsules.

[0052] Example 9 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that hydroxypropyl cellulose (Shandong Changfang Biotechnology Co., Ltd., with a hydroxypropoxy content of 61.6% and a viscosity of 727cp) of the same added concentration is used instead of hydroxypropyl methyl cellulose, while other conditions remain the same, to obtain intestinal flora capsules.

[0053] Example 10 This embodiment uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that hydroxyethyl cellulose (Jiangsu Weizhirun Biotechnology Co., Ltd., degree of deacetylation of 96.3, viscosity of 42cp) with the same added concentration is used instead of hydroxypropyl methyl cellulose, while other conditions remain the same, to obtain intestinal flora capsules.

[0054] Comparative Example 1 The comparative example uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that maltodextrin and hydroxypropyl cellulose are not added during the preparation of the intestinal flora capsules, while other conditions remain the same, to obtain intestinal flora capsules.

[0055] Comparative Example 2 The comparative example uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that maltodextrin is not added during the preparation of intestinal flora capsules, while other conditions remain the same, to obtain intestinal flora capsules.

[0056] Comparative Example 3 The comparative example uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that hydroxypropyl cellulose is not added during the preparation of the intestinal flora capsules, while other conditions remain the same, to obtain intestinal flora capsules.

[0057] Comparative Example 4 The comparative example uses the method provided in Example 1 to prepare intestinal flora capsules. The difference is that maltodextrin and hydroxypropyl cellulose are not added during the preparation of intestinal flora capsules, and trehalose (Shandong Yingneng Chemical Co., Ltd., product number YN-HZT-1) with a final concentration of 10 wt% is added. Other conditions are kept the same to obtain intestinal flora capsules.

[0058] Test case This test example illustrates the relevant performance of the gut microbiota capsules provided in the above embodiments and comparative examples. The specific tests include: 1. Collapse of intestinal flora capsules: Intestinal flora capsules were placed at 30℃ for 30 minutes, and the changes in the appearance of each capsule were observed. The results are shown in Table 2.

[0059] Table 2.

[0060] As shown in Table 2, compared with Comparative Examples 1-4, the simultaneous introduction of maltodextrin and hydroxypropyl cellulose in the method provided in Example 1 of this invention can effectively prevent the capsule from softening and collapsing, thus giving the obtained intestinal flora capsules better storage stability.

[0061] 2. Cryopreservation survival rate of intestinal flora: Intestinal flora capsules were frozen in a freezer at -80℃ for 72 hours. The number of viable bacteria in the mixed bacterial sludge before and after freezing was detected by flow cytometry, and the cryopreservation survival rate (%) was calculated according to the following formula. The results are shown in Table 3.

[0062] Cryopreservation survival rate = (Number of viable bacteria after cryopreservation / Number of viable bacteria before cryopreservation) × 100% Table 3.

[0063] As shown in Table 3, compared to Comparative Examples 1-4, the intestinal flora capsules prepared using the methods provided in Examples 1-10 of this invention maintained an intestinal flora survival rate of over 79.06% after being frozen at -80°C for 72 hours. This indicates that the specific concentrations of maltodextrin and cellulose ether derivatives work together to achieve an excellent protective effect on the intestinal flora.

[0064] 3. Colonization rate of gut microbiota: 6-8 week old female Balb / c mice were used as experimental animals. After one week of acclimatization under the same conditions, they were randomly divided into 17 groups and subjected to the following treatments. (1) Blank control group (n=4): Enteric-coated capsule shells (excluding mixed bacterial sludge) were administered to mice at a dosage of 1 capsule per mouse; (2) Intestinal flora capsule group (14 subgroups in total, n=4 for each subgroup): Each intestinal flora capsule (0.02g / capsule of mixed bacterial mud) was administered to mice by gavage at a dosage of 1 capsule / mouse. (3) Hydroxypropyl methylcellulose group (n=4): 0.02 g / mouse of hydroxypropyl methylcellulose (Shandong Guangda Technology Development Co., Ltd., methoxy content of 28.2%, hydroxypropoxy content of 8.1%, viscosity of 51.2 cp) was administered to mice by gavage. (4) Maltodextrin group (n=4): 0.02 g of maltodextrin was administered to mice by gavage; After gavage, the mice in each group were fed under the same feeding conditions for 48 hours. The intestinal contents of each mouse were collected for 16S rDNA sequencing. The relative abundance of Firmicutes and Bacteroidetes in the intestinal flora of the mice was calculated and the average value was taken. The relative abundance of Firmicutes and Bacteroidetes in the intestinal flora of the mice before gavage treatment was used as a control. The results are shown in Table 4.

[0065] Table 4.

[0066] As shown in Table 4, compared with Comparative Examples 1-4, the relative abundance of Firmicutes and Nucleoides bacteria in the intestinal flora of mice was significantly increased after gavage treatment with the intestinal flora capsules prepared by the methods provided in Examples 1-10 of this invention. This indicates that the synergistic effect of maltodextrin and hydroxypropyl cellulose can simultaneously improve the colonization rate of Firmicutes and Nucleoides bacteria.

[0067] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention without departing from the principles and spirit of the present invention.

Claims

1. The application of the composition in the preparation of an intestinal flora colonization promoter, characterized in that, The composition consists of maltodextrin and cellulose ether derivatives added in a mass ratio of (10~40):(0.5~5).

2. The application according to claim 1, characterized in that, The composition promotes the colonization of Firmicutes and / or Bacteroidetes in the gut.

3. The application according to claim 1, characterized in that, The composition has at least one of the following technical features: (1) The cellulose ether derivatives are selected from one or more of hydroxypropyl methylcellulose, hydroxypropyl cellulose and hydroxyethyl cellulose; (2) The viscosity of the cellulose ether derivative is 40cp~60cp; (3) The DE value of the maltodextrin is 1%~25%.

4. A gut microbiota capsule, characterized in that, The intestinal flora capsule includes a mixed bacterial slurry, which is composed of maltodextrin, cellulose ether derivatives and intestinal flora slurry added in a mass ratio of (10~40):(0.5~5):(10~100).

5. The intestinal flora capsule according to claim 4, characterized in that, The gut microbiota capsule has at least one of the following technical features: (1) The cellulose ether derivatives are selected from one or more of hydroxypropyl methylcellulose, hydroxypropyl cellulose and hydroxyethyl cellulose; (2) The viscosity of the cellulose ether derivative is 40cp~60cp.

6. The intestinal flora capsule according to claim 4, characterized in that, The gut microbiota capsule has at least one of the following technical features: (1) The cellulose ether derivative is hydroxypropyl methylcellulose; (2) The methoxy content of the hydroxypropyl cellulose is 10%~30%, the hydroxypropoxy content is 1%~15%, and the viscosity is 40cp~60cp.

7. The intestinal flora capsule according to claim 4, characterized in that, The DE value of the maltodextrin is 1%~25%.

8. The intestinal flora capsule according to claim 4, characterized in that, In the mixed bacterial sludge, the concentration of maltodextrin is 10wt%~40wt%, the concentration of cellulose ether derivatives is 0.5wt%~5wt%, and the concentration of intestinal flora sludge is 55wt%~89.5wt%.

9. The method for preparing the intestinal flora capsule according to any one of claims 4 to 8, characterized in that, The preparation method includes: mixing maltodextrin, cellulose ether derivatives and intestinal flora sludge evenly to obtain mixed sludge; filling the mixed sludge into capsule shells to obtain the intestinal flora capsules.

10. The use of the intestinal flora capsule according to any one of claims 4 to 8 in the preparation of a drug for flora transplantation.

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