8 results about "Intestinal villus" patented technology

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

This invention discloses a strain of *Bacillus belyceae* with broad-spectrum antibacterial activity and its application in the prevention and treatment of necrotic enteritis in poultry. The *Bacillus belyceae* strain provided by this invention (… Bacillus from Velez The genome of strain GDG25CB056 is approximately 3.91 Mb in size, contains no virulence factors, and is rich in gene clusters encoding non-ribosomal peptides and polyketide synthases. Studies have confirmed that this strain exhibits significant growth advantages, strong tolerance to high temperatures, gastric acid, and bile salts, which facilitates intestinal colonization. It can produce siderophores and various enzymes, and has overcome the technical bottleneck of a narrow antibacterial spectrum, possessing broad-spectrum antibacterial activity, especially strong specific antagonistic activity against Clostridium perfringens. In vivo experiments have shown that this strain can significantly reduce the Clostridium perfringens load in the cecum of chicks with necrotizing enterocolitis, alleviate intestinal congestion and swelling, repair damaged intestinal villi, and enhance mucosal barrier function, providing a safe and efficient strain resource for the prevention and treatment of bacterial enteric diseases.

The application belongs to the technical field of organ chip models, and specifically discloses a liver-intestine chip model and a method for evaluating the influence of oligosaccharides on the liver by using the same. The liver-intestine chip model comprises a multilayer microfluidic structure, the main body material of the multilayer microfluidic structure is modified polydimethylsiloxane, the multilayer microfluidic structure comprises an intestinal microcavity, a liver microcavity and a microchannel connecting the two, the intestinal microcavity is inoculated with intestinal epithelial cells, the liver microcavity is inoculated with liver cells, the bottom of the liver microcavity is provided with a window array, the corner of the liver microcavity is provided with a groove, the bottom of the intestinal microcavity is integrated with an elastic membrane, the elastic membrane is communicated with an external air pressure driving system, and the upper surface of the elastic membrane is etched with a microstructure similar to small intestinal villi. By using the above liver-intestine chip model and the method for evaluating the influence of oligosaccharides on the liver by using the same, the liver-intestine organ chip model can simulate the microenvironment of the intestinal-liver axis in vivo, and provides a more real and reliable platform for studying the influence of oligosaccharides on the liver.

This invention belongs to the field of microbiology technology, and specifically relates to the application of a strain of *Lactobacillus suis* HL1 derived from wild boars in Xinjiang and its feed additives. Lactobacillus saerimneri HL1, with accession number CGMCC No. 34386. This invention relates to *Lactobacillus suis* HL1, which exhibits good tolerance, strong antioxidant capacity, and effective antibacterial properties. It is sensitive to multiple antibiotics and promotes growth and development, enhancing intestinal villi growth, increasing nutrient absorption, and improving coat luster. This application isolates a potentially beneficial *Lactobacillus* strain with low or no drug resistance from wild boars in Xinjiang and evaluates its in vivo safety, laying the foundation for animal feed additives.

The application discloses a pig breed disease resistance breeding screening method based on taurine cholate metabolic markers, and belongs to the technical field of animal breeding. The application effectively screens the breeding pigs with stronger intestinal barrier function by establishing a breeding standard based on the intestinal barrier function difference, especially taking the taurine cholate level as a key index, and improves the health level and production benefit of pigs. Compared with the prior art, the ileum villus height to crypt depth ratio of the breeding pig is increased by 25.3%, the mRNA relative expression amount of ZO-1 and other tight junction proteins is increased by 32.7%, the SIgA and IgG levels are increased by 38.5%, the health level of the pig is significantly improved, and the production benefit is increased by 15.6%.

The application discloses application of caprylic capric glycerides in preparation of products for relieving or inhibiting intestinal cell iron death of piglets. It is found that the caprylic capric glycerides have a regulating effect on intestinal cell iron death of piglets, and feeding piglets with products containing the caprylic capric glycerides can relieve or inhibit intestinal epithelial cell iron death of piglets, protect the intestinal morphology and function of piglets, and relieve or inhibit intestinal cell iron death of piglets induced by Escherichia coli K88. Feeding piglets with feed products containing the caprylic capric glycerides can positively regulate the jejunal villus height and the ratio of the jejunal villus height to the crypt depth of piglets, the ultrastructure of the jejunal of piglets, the protein expression amount of the tight junction protein ZO-1 of the jejunal of piglets, the contents of 4-HNE, GPX4, MDA and GSH in the jejunal of piglets, and the protein expression amount of the iron death signal path related factors (FTH1, GPX4, SLC3A2 and TFR1) of the jejunal of piglets, and can positively and comprehensively protect the intestinal health of piglets.

This invention relates to the field of in vitro intestinal organoid culture technology, and discloses an in vitro method for constructing a small intestinal organoid model of the Chinese red panda. The method involves collecting ileum tissue from Chinese red pandas that have died due to disease or accident, and then using PBS... + After rinsing, the tissue was placed in tissue transport buffer for low-temperature transport; the following steps were performed sequentially: intestinal lumen rinsing, removal of external tissue, longitudinal incision, scraping of intestinal villi onto a glass slide, and pre-cooling with PBS. + The tissue was repeatedly inverted and washed until the supernatant was clear. It was then minced, digested with EDTA digestion solution, and the crypts were separated by mechanical pipetting. After filtration through a sieve, the crypt precipitate was collected by centrifugation. The crypt precipitate was resuspended, its concentration adjusted, and mixed with Matrigel. It was then inoculated into culture plates, and after the Matrigel solidified, complete culture medium was added for further culture to obtain small intestinal organoids from the Chinese red panda. A small intestinal organoid model was successfully constructed and can be stably passaged in vitro, filling a gap in the technology for constructing small intestinal organoids from the Chinese red panda.