48 results about "Aesculetin" patented technology

The invention relates to a method for preparing ash bark coumarin compound. Wherein, it boils and extracts with water, uses porous resin to separate and purify ash bark coumarin compound; and said compound comprises aesculin, aesculetin, fraxin, and fraxetin, while the total coumarin content is higher than 50%, and the contents of aesculin, aesculetin, fraxin, and fraxetin are higher than 40%, while their ratios are 4-6:1-3:2-4:1. The invention also provides its application in medicine production.

The invention discloses a high performance capillary electrophoresis (HPCE) quick quantitative method for four active components of fraxin, esculin, fraxetin and aesculetin in cortex fraxini or extract thereof. By the method provided by the invention, four cortex fraxini coumarins are well separated and determined in 10min, and simultaneously all the components have good linear relations and highrecovery rates. The method provided by the invention has good relativity of determination results compared with a high performance liquid chromatography (HPLC) method, greatly shortens the analyzing time, obviously reduces environmental pollution, notably decreases the analyzing and operating cost, provides a quick efficient analyzing means for researching the distribution of the active components of cortex fraxini and the variation rule of content in different harvest phases, and also can be used for researching the inherent quality of the cortex fraxini extract and for referencing in development research of new drugs.

The present invention provides a new application of Fraxinus general counmarin and aesculetin as the extractives of Chinese medicine material cortex Fraxini. The said two has obvious effects of stopping pain, resisting inflammation and reducing blood uric acid. They has obvious curative effect on acute and chronic gout and no obvious toxic side-effect. The present invention also provides a simple preparation process of Fraxinus general coumarin, and that is a Fraxinus general coumarin concentrating and purifying process.

An anti-inflammatory tablet HPLC fingerprint construction method comprises the following steps: 1, preparing a test solution: grinding an anti-inflammatory tablet, weighing the ground tablet, placing the ground tablet in an extractor, adding petroleum ether, carrying out hot reflux extraction, removing the petroleum ether, adding methanol, carrying out ultrasonic treatment, supplementing methanol, and filtering the obtained solution; 2, preparing a reference solution: taking a chlorogenic acid reference substance, an aesculetin reference substance, a scutelloside reference substance, a linarin reference substance, a baicalein reference substance and a wogonin reference substance; and 3, determining: respectively taking the reference solution and the test solution, respectively injecting the reference solution and the test solution to a liquid chromatograph, recording the chromatogram in 120min, and processing the chromatogram through using fingerprint software to obtain the fingerprint of the anti-inflammatory tablet. The method has the advantages of establishing the common mode of the HPLC characteristic fingerprint of the anti-inflammatory tablet, calibration of 27 common peaks, effective characterization of the quality of the anti-inflammatory tablet, overcoming of unicity and one-sidedness of original quality control methods, and high application values.

The invention belongs to the field of veterinary drugs, and discloses a medicine composition used for controlling poultry airsacculitis. The composition is composed of the components of, by weight: 10-20% of aesculetin, 10-20% of deoxycycline, 10-20% of colistine sulfate, 2-5% of levamisole, 10-30% of vitamin C, 0.01-0.015% of dexamethasone, and balance of auxiliary materials. The invention also provides a preparation method of the medicine composition. The medicine composition provided by the invention has high effect against poultry airsacculitis, wherein an effective rate can be higher than 95%. The medicine composition is also advantaged in fast effect. In a first day of medication, a substantial effect can be provided, and the disease can be cured in 4 days. Also, the medicine composition has a wide antimicrobial spectrum. The medicine composition has excellent killing effects against poultry airsacculitis pathogens such as Escherichia coli, mycoplasma, pseudomonas aeruginosa, and the like. The medicine composition can effectively control secondary bacterial infections. Further, the medicine composition provided by the invention is advantaged in simple production method and convenient application. The production method is labor-saving.

The invention provides a detection method of caffeic acid and aesculetin at the same time in a dandelion and viola philippica granules or an intermediate extract thereof. Through multiple experiment and research, chromatographic conditions of HPLC in detection of the two ingredients at the same time are screened and explored and a content determining method by which the two ingredients in the dandelion and viola philippica granules or the intermediate extract thereof can be detected at the same time. Under the chromatographic conditions, chromatographic peaks of the two ingredients can be separated successfully, interferences of impurity peaks are avoided, a retain time is proper and peak shapes are excellent. By means of the method, an object of successfully determining the contents of the two ingredients can be achieved and a basis of quality researching of a preparation is established. The detection method also provides a reference method of quantitative researching of a preparation containing the caffeic acid and the aesculetin at the same time.

The invention discloses application of aesculetin to preparation of a medicine or a health-care product for treating and preventing eye diseases. The invention defines and finds out that the anti-oxidization effect of the aesculetin has a protection effect on eye cells for the first time. The aesculetin has a remarkable anti-apoptotic treatment effect on the eye diseases comprising oxidative damage pathogenic factors and is a compound with a development prospect; a novel medicine is provided for clinically treating diseases related to eye oxidative damage and apoptosis.

The invention discloses aesculetin with anti-oxidative and anti-aging activity as well as a preparation method and an application of aesculetin. The preparation method comprises preparation steps as follows: (1), cortex fraxini residues are dried, sterilized for 30 min under ultraviolet irradiation and taken out; (2), distilled water which is 8 times the sterilized cortex fraxini residues in parts by weight is added to the sterilized cortex fraxini residues for fermentation, after fermentation, a sample is taken out, dried, smashed and screened by a 30-mesh sieve, and fermented cortex fraxini residue powder is obtained; (3), an organic extracting agent is added to the fermented cortex fraxini residue powder for reflux extraction, extracting solutions are combined, a combined solution is filtered, and the organic extracting agent is subjected to reduced-pressure recovering and concentrated into thick paste; (4), distilled water is added to the thick paste for dilution, heating dissolution is performed, an obtained solution is filtered while still hot, and a filtrate is separated, purified and decolorized and then is subjected to reduced-pressure concentration and freeze drying finally. Aesculetin has a function of eliminating oxygen free radicals, has a protecting effect on cell DNA oxidative damage caused by lipid peroxide and can effectively delay skin cell senescence.

The invention discloses a traditional Chinese medicinal composition, Qinpi Jiegu Capsules, and a quality detection method of a preparation of the composition, belonging to the technical field of medicine. The existing traditional Chinese medicinal composition, Qinpi Jiegu Capsules, and the quality standards of the preparations of the composition are improved, the identification method of ash bark is improved on the basis of the existing standard, and an identification method of Pyrethrum tatsienense is added. The method is used for measuring the contents of aesculin and aesculetin in the ash bark at the same time under a same mobile phase, and a method for measuring the content of sipeimine in Fritillaria cirrhosa in the preparations is added, so as to further ensure safe, uniform, stable and controllable product quality and ensure the clinic curative effect of the composition and the administration safety of patients.

The invention relates to the technical field of traditional Chinese medicines, in particular to philippine violet herb dry paste powder and an extraction method as well as a philippine violet herb capsule and a production method. The extraction method of the philippine violet herb comprises the following steps: (1) primary decoction; (2) secondary decoction; (3) concentration under reduced pressure; (4) vacuum drying. The invention adopts a rapid decoction extraction method to ensure the complete extraction of antibacterial active ingredients, and improve the efficacy of medicines and the utilization rate of medicinal materials, and discusses influencing factors, and optimizes a test, the finally extracted philippine violet herb dry paste powder has a relatively high content of aesculetin,and the extraction method is simple and effective, and easy to promote. In addition, the invention also provides a capsule using the philippine violet herb dry paste powder as a sovereign drug. The capsule is reasonably compatible in the whole formula, and has the effects of clearing away heat and inducing diuresis, promoting blood circulation to remove stasis, and dredging collaterals and regulating menstruation, and proved by modern pharmacodynamics research and experiments, the capsule can be used for treating various prostatitis, especially for chronic prostatitis, with a significant effect.

The invention discloses a compound tilmicosin solid dispersing agent and a preparation method thereof. The compound tilmicosin solid dispersing agent comprises, by weight percentage, 1.0-2.0% of Eudragit L-100, 0.2-0.5% of sodium bisulfite, 10-30% of tilmicosin, 0.5-1% of lysozyme, 0.5-1% of aesculetin, 2-5% of polyvidone and the balance anhydrous dextrose. The solid dispersing agent prepared by the method has the advantages that the medicine effect of the tilmicosin is kept, bioavailability is increased evidently, good palatability is achieved, gastrointestinal tract irritation is avoided, cure rate can be increased greatly, controlled-release absorption is formed in the intestinal tract, plasma concentration is kept at the therapeutic dose for a long time, and the highest medicine utilization rate is achieved; in addition, the dispersing agent has an immunoregulation function and can recover macrophage immune activity.

The invention relates to a method for extracting high-purity aesculetin from euphorbia lathyris fruit. The method for extracting high-purity aesculetin from the euphorbia lathyris fruit comprises the following steps of: squeezing oil from the obtained euphorbia lathyris fruit so as to obtain cake residues, and soaking or refluxing the cake residues for 0.5-72 hours twice to three times by using an organic solvent at 10-60 DEG C, thus obtaining an organic solvent leaching liquor of the euphorbia lathyris fruit; concentrating the organic solvent leaching liquor to obtain an extract; dissolving the extract by using a right amount of low-polarity organic solvent, filtering to obtain precipitates, repeatedly dissolving the precipitates by using the low-polarity organic solvent twice to three times, and filtering to obtain the precipitates; washing the precipitates obtained in the end by using a small amount of acetone twice to three times, filtering to obtain pale yellow precipitates, and drying the prepared faint yellow precipitates to obtain the aesculetin with the purity higher than 95%. The method has the advantages of rapidness, high yield, low cost and the like. The purity of the prepared aesculetin is higher than 95%, and the yield of the aesculetin can reach up to 0.12%.

The present invention provides a new application of Fraxinus general counmarin and aesculetin as the extractives of Chinese medicine material cortex Fraxini. The said two has obvious effects of stopping pain, resisting inflammation and reducing blood uric acid. They has obvious curative effect on acute and chronic gout and no obvious toxic side-effect. The present invention also provides a simple preparation process of Fraxinus general coumarin, and that is a Fraxinus general coumarin concentrating and purifying process.

The invention discloses a natural product electrolyte composition comprising a nitrogen-containing derivative of a polyphenol, an anthracene phenol compound, an alizarin derivative, an amino acid derivative and an aesculetin. In addition, the invention also discloses the use of the above composition for an electrolyte of a zinc-manganese battery, and a zinc-manganese battery in which the electrolyte comprises the composition. The composition of the invention has excellent corrosion inhibition effect, good conductivity, low temperature resistance and high temperature resistance; When used as the electrolyte of the zinc-manganese battery, the self-discharge performance of the negative electrode material can be suppressed in the non-working state, thus the self-loss of the battery can be reduced, and the service life of the battery can be greatly prolonged. However, the normal battery performance can be exerted due to the good electric conductivity in the battery operation. More importantly, the composition does not contain heavy metal mercury, avoiding heavy metal pollution that may be caused by discarded batteries, and reducing follow-up treatment costs and environmental pollution risks.

The invention discloses a method for extracting aesculetin from caper euphorbia seeds, belonging to the technical fields of medicine and the chemical industry. The content of aesculetin in a natural plant, i.e., ash bark, is low; however, the content of aesculetin in the raw material caper euphorbia seeds in the invention is high. Since the ash bark has low aesculetin content, traditional separation and extraction technology is difficult in producing aesculetin with purity of 98% or above and realizing high-yield industrial production at the same time. The invention provides the method for separating and extracting aesculetin from the caper euphorbia seeds; with the method, cost for industrial production is reduced, and the purity of aesculetin reaches the grade of a bulk drug; so the method improves production efficiency, and is green and environment-friendly in process and free of severe harm to environment.

The invention discloses an application of aesculetin in preparation of a medicine for treating osteoporosis. The aesculetin provided by the invention can be used for obviously improving the bone mass level of an ovariectomized rate osteoporosis animal model, and simultaneously reducing the activity of an osteoclast, thereby proving that the aesculetin has an obvious treatment effect on the post-menopause osteoporosis.

The invention provides a traditional Chinese medicine disinfectant and a preparation method thereof, and relates to the field of disinfectant. The traditional Chinese medicine disinfectant is prepared from, by weight, long pepper, litharge, Dendrobium, bee venom, emblic leaf, yellow root, saururus chinensis, aesculetin, camphortree leaf, swallow tail, rambutan, mile swertia herb, fungus-infected rice spike, chicory, senna, Celandine, aloe, gallnut, gentian, honeysuckle, Fructus Cnidii and mugwort; the preparation method includes steps of (1), weighing raw materials; (2), preparing extracting fluid and mixed powder; (3), mixing. The traditional Chinese medicine disinfectant solves the problem that the existing disinfectant is irritating to the wound and skin of a human body and even can cause the anaphylactic reaction of human body.

A method for promoting differentiation of clonogenic neural stem cells (NSCs), comprising administering to a patient in the need of such promoting a coumarin compound represented by formula I or by formula II. The representative coumarin compounds include 7-hydroxycoumarin, daphnoretin, scopoletin, edgeworin, aesculetin and esculetin-6-β-D-glucopyranoside. The coumarin compounds showed significant activity of directing the differentiation of NSCs in pharmacological test and thereof could be used to prepare drugs to direct NSCs differentiated to oligodendrocyte progenitor cells (OPCs) for the treatment of demyelinating diseases or spinal cord injury. The drug could be a pure coumarin compound or a pharmaceutical composition comprising a therapeutical dose of a coumarin compound as active ingredients and a pharmaceutically-acceptable carrier. The content of the active ingredients in the pharmaceutical composition is between 0.1% and 99.5% by weight.

The invention discloses an establishment method for a variable-wavelength fingerprint spectrum of Chinese pulsatilla root decoction granules. The method comprises the following steps of (1) preparing reference substance solutions: preparing reference substance solutions of berberine hydrochloride, palmatine hydrochloride, coptisine, jatrorrhizine hydrochloride, aesculin, aesculetin and pulchinenoside anemoside B4 respectively; (2) preparing a test sample solution: weighing the Chinese pulsatilla root decoction granules, extracting the Chinese pulsatilla root decoction granules and filtering an extracted solution by using a microporous membrane to obtain the test sample solution; (3) obtaining the fingerprint spectrum by determining with high performance liquid chromatography, wherein chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler by a chromatographic column; gradient elution is adopted; variable wavelengths are detected by ultraviolet light wavelengths; and (4) evaluating similarity: evaluating the fingerprint spectrum of the test sample by "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese medicine (Edition 2004A)". The method is simple, stable, high in precision and good in repeatability, and can identify the authenticity and merits of products accurately.

The invention provides a pharmaceutical composition of a cortex fraxini extract and borneol as well as a preparation method and application of the pharmaceutical composition. The pharmaceutical composition comprises 3.6-116 parts by weight of the cortex fraxini extract and 1 part by weight of borneol. The cortex fraxini extract mainly takes cortex fraxini total coumarin as an active ingredient, and the cortex fraxini total coumarin comprises aesculin, aesculetin, fraxin and fraxetin; the content of cortex fraxini total coumarin in the cortex fraxini extract is 75-85 wt% or above, the sum of the contents of aesculin, aesculetin, fraxin and fraxetin in the cortex fraxini extract is 55-65 wt% or above, and the content ratio of aesculin to aesculetin to fraxetin is (30.0-40.0): (2.0-4.0): (20.0-30.0): 1. The cortex fraxini extract is prepared by taking traditional Chinese medicine cortex fraxini decoction pieces or a cortex fraxini medicinal material as a raw material and adopting a combined process of alcohol extraction, macroporous resin column purification and the like. The composition disclosed by the invention can be used for remarkably inhibiting the formation of newborn lymphatic vessels in corneas and has a relatively good treatment effect on diseases such as corneal injury.

The invention discloses a quick-healing cream and a preparation method thereof. The quick-healing cream takes porous nano-silica microspheres coated with aesculetin and herba Agrimoniae extract, A myrrh resin extract, creatine and adenosine triphosphate are the main active materials, As that nutrient such as milk protein and serine are added, various natural antioxidant and meridian activating substances such as leonurus japonicus, borneol, pepper seed extract and angelica sinensis extract are combine, and the natural nutrients are organically combined to play a better effect through scientific material selection and reasonable collocation. The quick-healing cream provided by the invention can be absorbed through the skin, and the quick-healing cream can resist oxidation, promote metabolism and cell repair, and improve the smoothness and fineness of the human skin to achieve the anti-aging effect. In addition, the Su Yu Cream provided by the invention can dredge meridians, promote blood circulation, remove blood stasis, subside swelling and relieve pain, and has therapeutic effect on sub-health symptoms such as cervical spondylosis and scapulohumeral periarthritis.

The invention relates to a method for extracting high-purity aesculetin from euphorbia lathyris fruit. The method for extracting high-purity aesculetin from the euphorbia lathyris fruit comprises the following steps of: squeezing oil from the obtained euphorbia lathyris fruit so as to obtain cake residues, and soaking or refluxing the cake residues for 0.5-72 hours twice to three times by using an organic solvent at 10-60 DEG C, thus obtaining an organic solvent leaching liquor of the euphorbia lathyris fruit; concentrating the organic solvent leaching liquor to obtain an extract; dissolving the extract by using a right amount of low-polarity organic solvent, filtering to obtain precipitates, repeatedly dissolving the precipitates by using the low-polarity organic solvent twice to three times, and filtering to obtain the precipitates; washing the precipitates obtained in the end by using a small amount of acetone twice to three times, filtering to obtain pale yellow precipitates, and drying the prepared faint yellow precipitates to obtain the aesculetin with the purity higher than 95%. The method has the advantages of rapidness, high yield, low cost and the like. The purity of the prepared aesculetin is higher than 95%, and the yield of the aesculetin can reach up to 0.12%.

The invention relates to a method for separating and purifying fraxetin and aesculetin in cortex fraxini through high-speed counter-current chromatography. The method comprises the following steps: (1) crushing the cortex fraxini medicinal material, heating and extracting by adopting an ethanol solution, merging filtrate, and carrying out reduced-pressure rotary evaporation to obtain a crude cortex fraxini extract; (2) dissolving the cortex fraxini crude extract obtained in the step (1), extracting with petroleum ether, ethyl acetate and n-butyl alcohol respectively, filtering extract liquor, and concentrating under reduced pressure to obtain petroleum ether, ethyl acetate, n-butyl alcohol and a water extraction section respectively; and (3) separating and purifying the ethyl acetate extraction section by adopting high-speed counter-current chromatography to obtain the fraxetin and the aesculetin. The method overcomes the defects of complex operation, sample adsorption loss, low recovery rate and the like of the traditional preparation method; and the method is high in efficiency, easy to operate, large in preparation amount and low in comprehensive cost, and has good application and popularization value.

The invention discloses a method for determining the content of multi-component chemical components in Chinese pulsatilla root decoction. The method specifically comprises the following steps: respectively establishing standard curves of anemosides B4, B5, D and A3, hederasaponin A1, alpha-hederasaponin, aesculin, aesculetin, fraxetin, phellodendrine, palmatine hydrochloride, coptisine hydrochloride and berberine hydrochloride by utilizing an HPLC-MSMS (High Performance Liquid Chromatography-Mass Spectrometry Mass Spectrometry) analysis method; measuring a Chinese pulsatilla root decoction sample extracted by a decoction method, making a standard curve, and measuring the content of the 13 chemical components. A scientific basis is provided for quality control of the Chinese pulsatilla root decoction and related preparations thereof.

The invention belongs to the field of biological prevention and treatment of plant diseases, and discloses application of a plant-derived compound aesculetin in prevention and treatment of plant oomycete diseases for the first time. Tests prove that under the in-vitro condition, the aesculin has a very strong inhibition effect on hypha growth and spore germination of phytophthora capsici, phytophthora sojae, phytophthora infestans, phytophthora nicotianae, pythium aphanidermatum, pythium ultimum and grape downy mildew. Meanwhile, living body test results also show that aesculetin has a good prevention and treatment effect on pepper phytophthora blight, rape damping-off and grape downy mildew caused by the plant pathogenic oomycetes, and can be used for preventing and treating oomycetes diseases. The method has the advantages: 1, the aesculetin is used for preventing and treating oomycete diseases, so that the pollution to the environment is small, the residue on crops is low, and the method is relatively safe to non-targets; 2, the aesculetin is used for preventing and treating oomycete diseases, so that the use amount of chemical pesticides can be reduced, and the problem of drugresistance is relieved; 3, the aesculetin is rich in source, so that the cost for developing a prevention and treatment medicament taking aesculetin as a main component is relatively low;

The invention discloses a traditional Chinese medicinal composition, Qinpi Jiegu Capsules, and a quality detection method of a preparation of the composition, belonging to the technical field of medicine. The existing traditional Chinese medicinal composition, Qinpi Jiegu Capsules, and the quality standards of the preparations of the composition are improved, the identification method of ash bark is improved on the basis of the existing standard, and an identification method of Pyrethrum tatsienense is added. The method is used for measuring the contents of aesculin and aesculetin in the ash bark at the same time under a same mobile phase, and a method for measuring the content of sipeimine in Fritillaria cirrhosa in the preparations is added, so as to further ensure safe, uniform, stable and controllable product quality and ensure the clinic curative effect of the composition and the administration safety of patients.

The invention belongs to the technical field of traditional Chinese medicinal material detection, and particularly relates to a method capable of simultaneously determining contents of various chemical components in cortex acanthopanacis medicinal materials. The method comprises the following steps: (1) chromatographic conditions; (2) preparation of a reference solution; (3) preparation of a testsolution; (4) determination and the like. The method capable of simultaneously determining contents of various chemical components in cortex acanthopanacis medicinal materials can simultaneously determine the contents of four chemical components including chlorogenic acid, aesculetin, isochlorogenic acid A and isochlorogenic acid C in the cortex acanthopanacis medicinal material; the detection method is simple, high in accuracy, good in stability and good in reproducibility, the quality of the cortex acanthopanacis can be evaluated more comprehensively and accurately, the quality of the medicinal material can be effectively controlled, and a basis can be provided for comprehensively evaluating and controlling the quality of the cortex acanthopanacis medicinal material.

The invention discloses a direct electrochemical method for detecting aesculin and / or aesculetin, which is characterized in that a sample is detected by using a carbon fiber electrode PtNPs / AuNPs / CFME modified by AuNPs and PtNPs through cyclic voltammetry or differential pulse voltammetry. The electrode used in the method has good electrochemical response, one component or both can be accurately detected in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with interference of other impurities or in a sample with two samples, detection results are accurate, detection limit is low, and detection sensitivity and selectivity can be improved in complex biological matrixes.