The invention provides a composition for relieving and treating eyestrain caused by electronic radiation, and a preparation method and application thereof. The composition is mainly prepared from cameliva sativa extract, an anthocyanin extract, a rhodiola rosea extract, a cassia seed extract, a feather cockscomb seed extract, a mushroom extract and radix rehmanniae extract into different dosage forms and different foods. The composition has the advantages that the raw materials are all natural, easy to obtain and cheap, effective components of natural products are enriched through extraction,and the extraction process is simple and free from pollution; the cameliva sativa extract is extracted by taking squeezed cameliva sativa meal as a raw material, influences of the cameliva sativa mealas garbage on the environment are avoided, the cost of the raw material is greatly reduced, resource recycling is realized, and the composition has a good medicinal effect; products prepared from thecomposition can be presented in various forms, are convenient to use and safe to take, can effectively relieve and treat the eyestrain, resist radiation and improve immunity, and have few clinical side effects but significant social and economic benefits.