22results about How to "Reduce peroxidation" patented technology

The invention discloses a preparation method of sandwich type immunomagnetic beads with high thermal stability, which comprises the following steps: by taking polystyrene microspheres as a template, introducing oil-phase Fe3O4 nanoparticles into the polystyrene microspheres through a swelling method, and then carrying out hydrolytic condensation reaction on the surface of a microsphere assembly by utilizing n-octyltrimethoxysilane OTMS to obtain the sandwich type immunomagnetic beads with high thermal stability. The preparation method comprises the following steps: carrying out surface amination modification on a silicon dioxide thin layer which is grown by hydrolysis of tetraethoxysilane TEOS to realize coating of an outermost organic shell layer, carrying out surface amination modification, and carrying out cross-linking on 1, 4-butanediol diglycidyl ether and erythritol on the surface to form an epoxy polymer layer so as to obtain the sandwich-type immunomagnetic bead with a cross-linked epoxy polymer layer introduced to the outer layer. According to the epoxy group modification process on the surface of the magnetic bead, a direct cross-linking modification process is adopted, so that the operation steps are simplified, and the obtained epoxy group magnetic bead is ensured to show excellent biocompatibility. In addition, the method omits the tedious process of functional group activation, and allows direct coupling with the antibody, thereby greatly improving the efficiency and convenience of the experiment.

The invention discloses an endophytic fungus and application thereof, the endophytic fungus strain is 0522-8-2, the classification name is Aspergillus tubingensis, the preservation unit is China General Microbiological Culture Collection Center, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, 3 #, 1 # yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is January 3, 2024, and the preservation number is CGMCC No.41026; the salt-tolerant plant root endophytic fungi 0522-8-2 can obviously improve the survival rate of forest tree cuttings under salt stress and relieve the adverse effects of salt stress on the growth traits, physiology and photosynthesis of host roots; phosphorus is dissolved and alkali is controlled by secreting organic acid, so that absorption of a host plant to insoluble phosphorus is improved, and meanwhile, the adverse effect of high pH of saline-alkali soil on plant growth is relieved; therefore, the method is suitable for popularization and application.

The invention discloses a lactobacillus johnsonii and related products and applications thereof, and relates to the field of microorganisms. The Lactobacillus johnsonii GJ231 provided by the invention has a preservation number of CCTCC NO: M 2023981. Compared with other lactobacillus johnsonii, GJ231 is acid-resistant and bile-resistant, has a higher survival rate in the intestine, has a better antibacterial effect on a variety of pathogens, and can be used to prevent and treat related diseases caused by pathogens; secondly, GJ231 has a better high temperature tolerance and has a higher survival rate in the process of spraying and preparing pet food; furthermore, GJ231 also has a strong antioxidant capacity, can reduce the peroxidation reaction caused by oxidative stress, and can be used for anti-aging and anti-oxidation; in addition, GJ231 also has a bacteriocin gene cluster, which may be used to produce bacteriocin, and has the potential to replace antibiotics and food preservation.

The invention discloses a carnation heat shock transcription factor as well as a coding gene DcHsfA2 and application thereof. The dianthus caryophyllus heat shock transcription factor provided by the invention is a protein with an amino acid sequence as shown in SEQ ID NO: 2. The invention also provides an encoding gene DcHsfA2 of the carnation heat shock transcription factor. The invention also provides a method for preparing the transgenic plant, which comprises the following steps: introducing the coding gene into a starting plant to obtain the transgenic plant, compared with the starting plant, the heat resistance of the transgenic plant is improved. According to the invention, a molecular foundation is laid for improving the heat resistance of dianthus caryophyllus through a genetic engineering means, and a theoretical reference is provided for deeply researching the biological function and mechanism of the heat shock transcription factor in regulating and controlling the adversity stress resistance of plants.

The invention discloses an application of a PpWRKY53 transcription factor in improving heat resistance of Chinese pear fruits, the transcription factor is a positive regulatory factor of Chinese pears responding to high temperature stress, overexpression of the transcription factor PpWRKY53 gene in the Chinese pear fruits can significantly reduce hydrogen peroxide accumulation and membrane lipid peroxidation caused by high temperature, effectively alleviate water stain type browning symptoms of the fruits, and improve the heat resistance of the Chinese pears. The fruit hardness is improved. The invention provides a key gene resource for creating new germplasm of heat-resistant Chinese pears and breeding new varieties with high-temperature-resistant characteristics, and has important scientific and application values for ensuring stable production of summer Chinese pear industry and improving fruit quality and economic benefits.

The invention belongs to the technical field of biological medicines, and particularly relates to a thiophenol compound as well as a preparation method and application thereof. The prepared thiophenol compound is applied to human kidney cells HK-2 and living mice, it is found that the compound can remarkably inhibit peroxidation of cell membrane lipid molecules induced by hypoxia-reoxygenation and further induce cell ferroptosis, and the thiophenol compound has a long-term stable inhibition effect. By pretreating the compound, the levels of creatinine and urea nitrogen in serum after kidney injury can be remarkably reduced, which indicates that the compound can relieve acute kidney injury. Besides, the compound prepared by the invention is used for pretreatment, so that kidney external marrow region injury caused by ischemia-reperfusion is remarkably relieved, lesions such as renal tubular epithelial cell necrosis and the like are effectively relieved, and a new treatment strategy is provided for ferroptosis inhibitors when the compound is applied to kidney ischemia-reperfusion injury repair.

The invention discloses a composition for treating hyperlipidemia and a preparation method thereof. The composition comprises striga asiatica extract; the extract is any one or more of rhizoma pinellinae praeparata, radix pseudostellariae, anoectochilus formosanus and mangnolia officinalis. The hyperlipemia is spleen deficiency and phlegm dampness type hyperlipemia, and the extract is decocted with water and extracted. The medicine comprises the following components in parts by mass: 10 parts of striga asiatica, 10 parts of rhizoma pinellinae praeparata and 5 parts of anoectochilus formosanus. 15 parts of radix pseudostellariae, 10 parts of rhizoma atractylodis and 10 parts of cortex magnoliae officinalis; , 10 parts of fried hawthorn, 15 parts of Poria cocos and 10 parts of Gorgon fruit. Mixing and crushing the composition, and putting the crushed composition into a decoction bag; soaking the decoction bag in 10 times of cold boiled water for set time TA; preparing liquid A is formed; heating the prepared liquid A and the decoction bag with strong fire until boiling; preparing liquid B is formed; decocting with slow fire to prepare liquid B and a medicine decocting bag, and setting time TB; preparing liquid C; and filtering to prepare liquid C to obtain filtrate A and decoction dregs A. The filtrate A is the decoction for treating hyperlipemia.

The invention discloses procyanidine B2G2 from purple sweet potato Ningzi No.4 and application thereof, and belongs to the technical field of biological medicine, the molecular formula of the procyanidine B2G2 is C44H34O20, and the molecular weight of the procyanidine B2G2 is 882.736 Da. The procyanidine B2G2 remarkably relieves acute lung injury ALI by inhibiting inflammatory response and ferroptosis induced by klebsiella pneumoniae KP; experiments show that B2G2 can reduce the lung tissue injury score, the lung wet / dry ratio, the expression of inflammatory factors TNF-alpha, IL-6 and IL-1beta and the bacterial load, and up-regulate the expression of ferroptosis related proteins GPX4, SLC7A11 and HO-1; in addition, B2G2 is non-toxic to liver and kidney functions and has good safety, and the application provides a new drug candidate scheme for preparation of drugs for treating acute lung injury.

The invention belongs to the technical field of medicines, and particularly relates to application of agastache rugosus stomach-soothing powder in hangover alleviating and preparation of products for preventing and / or treating liver-stomach injury caused by alcohol. The wrinkled gianthyssop herb stomach-soothing powder has a remarkable protection effect on alcohol-induced acute drunkenness and related liver and stomach injuries, and the protection effect is related to ethanol metabolism enhancement, oxidation resistance improvement, lipid peroxidation reduction and inflammatory reaction inhibition of the wrinkled gianthyssop herb stomach-soothing powder. The composition of the agastache rugosus stomach-soothing powder has relatively high dosage efficiency and synergistic effect, plays a role in multiple aspects, and provides more comprehensive protection.

The invention relates to the field of food, in particular to a stabilizer for improving the stability of Chinese chestnut sauce, a preparation method of the stabilizer and application of the stabilizer in improving the properties of the Chinese chestnut sauce. In the stabilizer, trehalose has good water binding capacity and can reduce the water activity in a system and hinder recrystallization of starch, and hydroxyl groups of trehalose molecules can interact with the starch to form a stable structure and hinder movement of water molecules, so that the stability of the Chinese chestnut sauce is improved; palmitic acid in the palm oil is a saturated fatty acid, can maintain relatively high fatty acid unsaturated index and fatty acid unsaturation degree of the Chinese chestnut sauce, can relieve peroxidation of the Chinese chestnut sauce, effectively maintains integrity and stability of the Chinese chestnut sauce, and delays occurrence of browning of the Chinese chestnut sauce; sodium alginate and xanthan gum have good film-forming properties, and can effectively limit internal and external gas exchange and isolate microorganisms. Therefore, the stabilizer provided by the invention can improve the stability of the Chinese chestnut sauce.

This invention relates to a skin-brightening composition containing *Tea laurentii* essential oil, its preparation method, and its application. The composition comprises the following components: *Tea laurentii* essential oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil. The weight ratio of *Tea laurentii* essential oil, ascorbic acid peptides, oat β-glucan, camellia seed oil, and tiger nut oil is (0.1-10):(0.01-1):(0.01-5):(0.01-5):(0.01-5). The *Tea laurentii* essential oil is obtained by fermenting *Tea laurentii* tea leaves with *Aspergillus cristatus* followed by supercritical CO2 processing. 2 The composition obtained by extraction; the components of the present invention have synergistic effects, simultaneously blocking inflammatory roughness and dullness, breaking through the limitation of traditional products that only target dehydrated roughness, and providing a comprehensive solution for sensitive skin without hormone stimulation.

This invention belongs to the field of daily chemical products, specifically relating to a method for preparing hydrogenated castor seed oil and its skincare composition, comprising the following steps: S1. Crushing castor seeds and sieving them to obtain castor seed powder; S2. Treating the castor seed powder in a low-frequency pulsed electric field and intermittent microwave to obtain pretreated castor seed powder; S3. Mixing the pretreated castor seed powder with a compound enzyme preparation and performing subcritical extraction to obtain an extract; S4. Filtering the extract and distilling it to obtain a refined filtrate; S5. Mixing the refined filtrate with a palladium-on-carbon catalyst to obtain a semi-finished product; S6. Passing the semi-finished product through a fixed bed of activated carbon to obtain a primary filtrate, which is then filtered to obtain hydrogenated castor seed oil. The preparation method of this invention effectively retains the natural active ingredients in castor seed oil, thereby improving the effectiveness of the prepared hydrogenated castor seed oil, reducing organic solvent and heavy metal residues, and facilitating the application of hydrogenated castor seed oil in skincare compositions.

The invention discloses a steelmaking method for reducing comprehensive lime consumption by rapidly slagging a converter under high iron consumption, which belongs to the technical field of converter steelmaking and solves the technical problem of realizing converter slag-less smelting and improving the cleanliness level of molten steel under high iron consumption conditions. The steelmaking method of the invention comprises the following steps: S1, slag splashing and furnace protection; S2, adding limestone and sintered ore as bottom material; S3, adding molten iron and scrap steel into the converter; S4, adding lime, sintered ore and magnesium balls in batches when blowing 0min≤BT≤6min; S5, adding sintered ore to control the temperature of molten steel when blowing 6min<BT≤10min; S6, adding sintered ore to control the temperature of molten steel when blowing 10min<BT; S7, performing high oxygen pressure and low lance position oxygen lance operation for 40 to 60 seconds; S8, pouring the furnace to tap steel, and blocking slag at the end of tapping steel. By using the method of the present invention, the slag basicity is between 1.8 and 2.3 after 6 minutes of converter blowing, the dephosphorization rate is between 40 and 60%, and the dephosphorization effect in the early stage of the converter is remarkable.

The invention relates to application of fucoxanthine in preparation of a medicine for treating autoimmune hepatitis, the fucoxanthine serves as a natural carotenoid and has a remarkable liver protection effect, and the research reveals that the fucoxanthine reduces liver injury and ferroptosis in AIH by activating a mitochondrial autophagy signal channel to inhibit ferroptosis for the first time. Experimental results show that the fucoxanthine effectively inhibits ferroptosis by reducing lipid peroxidation, up-regulating expression of glutathione peroxidase 4 (GPX4), down-regulating expression of cyclooxygenase 2 (COX-2) and improving mitochondrial morphological change related to ferroptosis. The invention provides a new natural medicine choice for treating AIH, and provides powerful support for clinical treatment and prevention of autoimmune hepatitis.

The application relates to an application of a traditional Chinese medicine composition in a drug for chronic obstructive pulmonary disease, characterized in that the traditional Chinese medicine composition comprises the following components in parts: Magnolia officinalis 1-100 parts, Strychnos ignatii 1-100 parts, Toddalia asiatica 1-100 parts, Ephedra 1-100 parts, Amygdalus 1-100 parts, Notopterygium 1-100 parts, Zingiber officinale 1-100 parts, Pogostemonis 1-100 parts, Perilla 1-100 parts, Atractylodes 1-100 parts, Poria cocos 1-160 parts, Atractylodes macrocephala 1-120 parts, Gypsum fibrosum 1-100 parts, Fructus Crataegi 1-100 parts, Fructus Junci 1-100 parts, Fructus Hordei 1-100 parts, Lumbricus 1-100 parts, Radix et Rhizoma Veratri 1-100 parts, Herba Eupatorii Fortunei 1-100 parts, Semen Lepidii 1-100 parts and Semen Platycladi 1-100 parts.

The invention relates to the technical field of edible oil, and provides a camellia oil compound composition with a lipid-lowering function. The camellia oil compound composition provided by the invention comprises camellia oil, linseed oil and perilla oil. The camellia oil contains rich unsaturated fatty acid, oleic acid and linoleic acid, the flaxseed oil and the perilla oil contain a large amount of alpha-linolenic acid, and the camellia oil, the flaxseed oil and the perilla oil are compounded to obtain the compound composition which is rich in nutritional ingredients and has a good lipid-lowering effect; the compound composition can achieve the health-care function of lowering lipid while meeting the requirement of a human body for grease nutrition, and has a wide market prospect.

The present invention discloses a strain of Bacillus atrophaeus and its application, and relates to the technical field of plant biological pesticides. The Bacillus atrophaeus is classified and named as Bacillus atrophaeus, and the strain number is NWS-01. It has been deposited in the General Microbiological Center of China Microbiological Culture Collection Committee, and the deposit number is CGMCC No. 29755. In the present invention, the microbial agent is derived from the fermentation of Bacillus atrophaeus NWS-01, which can inhibit the growth of pathogenic fungi and reduce the incidence of plants; reduce the content of malondialdehyde in plants and enhance stress resistance; enhance plant photosynthesis, promote the accumulation of plant chlorophyll, and enhance the resistance and prevention ability of plants to diseases.

The invention relates to the technical field of converter smelting, in particular to a converter smelting method of low-iron-consumption low-temperature low-silicon molten iron, which comprises the following steps: (1) establishing a heat compensation model in the smelting process; (2) starting blowing and sparking for smelting, controlling the early-stage lance position to be at a first lance position value, controlling the oxygen blowing flow to be a first oxygen blowing flow value, increasing the oxygen blowing flow to a second oxygen blowing flow value when the to-be-blown oxygen amount is the first oxygen blowing value, and adding slag; (3) after the slag charge is added, increasing the oxygen blowing flow to a third oxygen blowing flow value, lowering the lance position to a third lance position value in the blowing process, and supplementing active lime into the furnace in batches; and (4) when the oxygen blowing amount meets the second oxygen blowing value, measuring the sublance, lowering the carbon pulling lance position to a fourth lance position value in the later period, meanwhile, increasing the oxygen blowing flow to a fourth oxygen blowing flow value, and when the oxygen blowing amount meets the third oxygen blowing value, lifting the lance, and ending blowing. According to the method, accurate temperature increasing and heat supplementing can be achieved, slagging is further promoted, dry returning in the blowing process is prevented, and the slagging and dephosphorization effects are guaranteed.

The present application belongs to the technical field of plant growth regulators, and particularly relates to a compound for relieving high-temperature and drought combined stress of plants, a plant growth regulator and application, wherein the compound is formed by mixing 2-amino-3-methyl hexanoic acid and gibberellic acid at a mass ratio of 0.025-0.25:1. The present application uses 2-amino-3-methyl hexanoic acid and gibberellic acid to treat wheat at the initial filling stage and corn at the silking stage, so that yield reduction can be significantly relieved, the antioxidant enzyme activity can be improved, the high-temperature and drought resistance of wheat and corn can be enhanced, and then the yield reduction can be significantly relieved, and the yield loss caused by high-temperature and drought combined meteorological disasters can be reduced.

The invention relates to an RH vacuum furnace treatment temperature control method which comprises the steps of molten iron pretreatment, converter blowing, RH treatment and continuous casting, in the converter blowing process, the sulfur mass content of end point molten steel is smaller than or equal to the upper limit of the sulfur mass content of a steel grade finished product, the temperature of the end point molten steel of a converter is 1630-1650 DEG C, and the mass content of free oxygen in the end point molten steel of the converter is 0.04-0.06%; and after tapping is finished, an aluminum-based modifier and lime are added into the molten steel, and slag materials in the molten steel are kept not to be accumulated. According to the method, the problems that in the RH single-link steelmaking process, the temperature required for converter tapping is high, and the oxidability of slag and the number of inclusions are increased due to oxygen blowing and temperature rising are solved.

This invention discloses the application of ZBTB7A as a target in the preparation of products for the diagnosis or treatment of sepsis-associated encephalopathy (SAE), belonging to the field of biomedical technology. This invention confirms that ZBTB7A can serve as a diagnostic biomarker and therapeutic target for SAE, providing strong technical support for the early diagnosis and treatment of SAE. Clinical sample validation shows that the expression level of ZBTB7A in peripheral blood astrocyte-derived exosomes of SAE patients is significantly downregulated, enabling early, non-invasive auxiliary diagnosis of SAE with high clinical diagnostic efficacy. This invention clarifies that high expression of ZBTB7A can effectively inhibit the release of pro-inflammatory factors, reduce lipid peroxidation and iron overload, improve the structural and functional integrity of mitochondria, protect neuronal and synaptic structures, and significantly reverse cognitive impairment.

The invention relates to the technical field of medicines, and particularly discloses application of an antioxidant alpha-lipoic acid in preparation of a medicine for improving gastritis. Chronic atrophic gastritis (CAG) is closely related to intestinal gastric cancer, wherein spasmolysis polypeptide expression metaplasia (SPEM) is a key node of pathological progress. Alpha-lipoic acid (ALA) is used for treating an SPEM mouse model, and the ALA is found to reduce lipid peroxidation and alleviate ferroptosis by activating an NRF2 antioxidant pathway, and significantly relieve SPEM symptoms and excessive generation of proinflammatory cytokines. According to the research, the protective effect of ALA on mouse SPEM is determined, and a new improvement choice is provided for improvement and regulation of SPEM. ALA, as a naturally existing antioxidant and anti-inflammatory agent, has a broad market prospect and social value in new application in protection of SPEM. The invention is expected to open up a new research direction for prevention and treatment of chronic atrophic gastritis.