47results about How to "Good in vitro antioxidant activity" patented technology

The present invention discloses an enteromorpha polysaccharide biological activity improving method comprising the following steps: enteromorpha polysaccharide is degraded by hydrogen peroxide-vitamin C combined method to obtain degraded enteromorpha polysaccharide (DEP); chloroacetic acid as a carboxymethylation reagent is reacted with the degraded enteromorpha polysaccharide (DEP) to prepare carboxymethylated enteromorpha polysaccharide (CDEP); and in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), the CDEP is coupled with hydroxylamine to obtain hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP). The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has a relatively low molecular weight (30-13kDa), and iron-chelating capacity is 0.2-1.2mmol / g. The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has high in-vitro antioxidant activity and antibacterial activity.

The invention discloses a method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum, which comprises the following steps: crush Rhizoma Polygonatum into 40-mesh granules, soak in an aqueous solution containing sodium citrate for 10-15 minutes, and dry; add 25-30 The ethanol solution with twice the weight is subjected to flash extraction to obtain the crude Polygonatum polyphenols extract; the crude Polygonatum polyphenols is eluted and purified by using a macroporous resin to obtain purified Polygonatum polyphenols. The present invention effectively reduces the degradation of polyphenols in Polygonatum polyphenols in the flash extraction process through the pretreatment of Polygonatum, improves the extraction rate of Polygonatum polyphenols; Carried out elution and purification, the purity of Polygonatum polyphenols was increased to more than 94.2%, which laid the foundation for the acquisition of polyphenolic compound monomers. At the same time, it also significantly improved the in vitro antioxidant activity of Polygonatum polyphenols extract, which is beneficial to Polygonatum Polyphenols Compounds Further Widely Utilized.

The invention relates to the technical field of food microbes, in particular to Lactobacillus helveticum ZJUIDS12 capable of improving alcoholic liver damage and its application. The invention discloses Lactobacillus helveticus ZJUIDS12, and its preservation number is CGMCC NO.23997. The invention also simultaneously discloses the application of the Lactobacillus helveticus ZJUIDS12 in the preparation of a product for protecting liver damage.

The invention discloses a method for preparing peanut antioxidant peptide through ultrasonic-assisted enzymolysis. The method comprises the following steps of: adding petroleum ether into peanut cake meal, leaching and degreasing at constant temperature, and drying to obtain degreased peanut cake meal; adding distilled water into the degreased peanut cake meal, and dissolving by using ultrasonic wave to obtain peanut cake meal suspension; regulating the pH value of the peanut cake meal suspension by using solution of hydrochloric acid, adding composite plant hydrolase ViscozymeL, performing ultrasonic-assisted enzymolysis to obtain enzymatic solution, deactivating enzyme, and cooling to room temperature; and regulating the pH value of the enzymatic solution by using solution of sodium hydroxide, adding protease, performing ultrasonic-assisted enzymolysis, deactivating enzyme, cooling to room temperature, centrifuging, and freeze-drying to obtain the peanut antioxidant peptide. In the method, the ultrasonic-assisted enzymolysis is utilized, so that the release amount of peptide in the enzymolysis process can be improved, the antioxidant activity of the obtained peanut antioxidant peptide is strengthened, and a theoretical foundation is provided for the development and application of peanut cake meal proteins.

The invention discloses a method for enhancing the bioactivity of hijiki polysaccharides, which comprises the following steps: the hijiki crude polysaccharides are placed in a buffer solution with a pH of 4.5 to 7.0 and acted on by a compound enzyme composed of pectinase and glucoamylase The degradation reaction was carried out under the following conditions; the obtained reaction solution was filtered after removing enzymes, and the obtained filtrate was concentrated and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 Da. Adopting the method of the invention can not only significantly improve the antioxidant activity and bile acid binding ability of the hijiki polysaccharide, but also enhance its immune regulation activity on macrophages.

The invention provides a preparation method of a skipjack oxidation resistant compound. The pH value of skipjack fresh slurry is adjusted to 7.0 to 8.0, after enzymolysis is conducted, enzymatic hydrolysate is centrifuged and filtered twice, and then the skipjack oxidation resistant compound is obtained. According to the two times of filtration, a ceramic membrane is used for filtration at the first time, and a nanofiltration membrane is used for filtration at the second time. The peptide constituent below 1000 Daltons in the skipjack oxidation resistant compound accounts for over 95% and is easy to absorb and digest; the reducing power is equivalent to that of 0.125 mg / mL of glutathione when the concentration is 5 mg / mL; and the IC50 value of scavenging activity of a 1,1-diphenyl-picrylhydrazyl free radical is 2.68 mg / mL, the IC50 value of scavenging activity of a superoxide anion free radical is 16.14 mg / mL, and the IC50 value of scavenging activity of a hydroxyl free radical is 21.04 mg / mL. The preparation process is simple in technology, and industrialization is easy. The product passes in vitro experiments and in vivo experiments, it is shown that the product has the good oxidation resistance effect, and the good development and use prospects are shown.