47results about How to "Good in vitro antioxidant activity" patented technology

The invention relates to an anti-oxidation mask, which comprises the following components in mass percentage: moisturizing agent: 10-25%, fullerene: 0.1-3%, quinoa extract: 0.1-5%, Alihong extract: 0.1- 5%, Thickener: 0.1‑3%, Conditioner: 0.1‑0.5%. The fullerene used in the present invention has good antioxidant properties, can effectively remove active oxygen free radicals, activate skin cells, and prevent aging; the quinoa extract is rich in polyphenols and flavonoids, and has good antioxidant properties in vitro Sex; Alihong extract contains chemical components such as flavonoids, polysaccharides, saponins and terpenes, which can improve the skin's immunity and play an anti-oxidation and anti-aging effect; there is a synergistic effect between the components in the formula, It has moisturizing, anti-oxidation and anti-aging effects.

The present invention relates to an anti-aging revitalizing face cream, which comprises the following components in mass percentage: vegetable oil: 1-8%, moisturizing agent: 10-28%, water-soluble fullerene: 0.01-5%, mulberry leaf flavonoid: 0.1-5% 5%, Cordyceps militaris extract: 0.1‑5%, emulsifier: 0.5‑3%, thickener: 0.1‑3%, conditioner: 0.1‑0.5%. The water-soluble fullerene adopted in the present invention has good antioxidant properties, can effectively remove active oxygen free radicals, activate skin cells, and prevent aging; both mulberry flavonoids and militaris extracts have good in vitro antioxidant properties, It can remove excess oxygen free radicals from the inside of the cells, play an anti-oxidation and anti-aging effect, and prevent free radicals from damaging the skin; there is a synergistic effect among the components in the formula, which has moisturizing, anti-oxidation, antibacterial, and anti-aging effects , Prevent fine lines and other effects.

The invention particularly relates to a method for extracting hovenia dulcis thunb total flavones by cooperation of a surfactant and a microwave-ultrasonic extraction process. The method comprises the following steps: (1) grinding hovenia dulcis thunb, and performing Soxhlet extraction by petroleum ether to remove fat so as to obtain fully defatted ground hovenia dulcis thunb; (2) uniformly mixing the ground hovenia dulcis thunb, distilled water and the surfactant to obtain a mixed solution; (3) performing microwave-ultrasonic extraction on the mixed solution at the microwave power of 200-600 W and the ultrasonic power of 100-500 W for 5-20 minutes; (4) after the extraction, performing alcohol precipitation on the extracted filtrate to remove alcohol supernatant, and concentrating and drying to obtain a hovenia dulcis thunb total flavones extract. Due to the adoption of the method for extracting hovenia dulcis thunb total flavones, the extraction time is greatly shortened, the extraction efficiency is improved, the cost is reduced, and the energy consumption is low; meanwhile, a distilled water solution in which a small amount of the surfactant is dissolved is taken as an extraction solution, so that the problem of toxic organic solvent residues is solved.

The cigarette with super low free radical content features its delayed released plant polyphenol microcapsule content of 0.02-0.6 wt% of shredded tobacco. The plant polyphenol microcapsule is first dissolved in water, alcohol, mixture of water and alcohol or water solution of propylene glycol and the solution is then sprayed to shredded tobacco. The delayed released plant polyphenol microcapsule may be catechin microcapsule, gallic acid microcapsule as degraded product of tannin or grape polyphenol microcapsule. The added plant polyphenol has stability raised by 9-12 times, extraneous antioxidant performance raised by 5-6 times and intracorporal antioxidant performance raised by about 10 times. Its effective component may be absorbed by oral cavity and respiratory system to eliminate free radical in body while meeting the need of smokers.

The invention discloses a method for improving the biological activity of sargassum fusiforme polysaccharide. The method comprises the following steps that crude sargassum fusiforme polysaccharide issubjected to a degradation reaction in a buffer solution with a pH value of 4.5-7.0 under the action of a complex enzyme composed of pectinase and glucoamylase; an obtained reaction liquid is filteredafter enzymes are removed, an obtained filter liquor is concentrated and then dialyzed by a dialysis bag with a molecular cut off of 3500 Da, an obtained dialyzed liquid is concentrated, frozen and dried to obtain sargassum fusiforme complex enzymolysis polysaccharide ESFP. The method not only can obviously improve the antioxidant activity and ability to be combined with cholic acid of the sargassum fusiforme polysaccharide, but also improve the immunomodulatory activity of the sargassum fusiforme polysaccharide to macrophages.

The invention belongs to the technical field of food biology, and particularly relates to an ultrasound-assisted extraction method of flavonoid compounds in citrus peel as well as research on the flavonoid compounds. The ultrasound-assisted extraction method of flavonoid compounds in citrus peel disclosed by the invention comprises the following steps: drying fresh citrus peel, and carrying out crushing to obtain citrus peel powder; adding an ethanol solution into the citrus peel powder, and performing ultrasonic extraction to obtain a flavone crude extract; and then, purifying the flavonoid compounds in the citrus peels by using macroporous resin to obtain a citrus peel extract containing the flavonoid compounds. The citrus peel extract containing the flavonoid compounds has high in-vitro antioxidant activity, and is capable of efficiently reducing blood glucose and blood fat; and moreover, the citrus peel extract can be efficiently fermented with intestinal flora to improve diversity of intestinal microorganisms.

The invention discloses an extraction and purification method of burdock tea polysaccharide. The method comprises the following main steps: extraction of polysaccharide by using a water extraction andalcohol precipitation method: weighing prepared burdock tea, pulverizing the burdock tea, putting the pulverized burdock tea into a container, adding water, carrying out uniform mixing, conducting concentrating by using a rotary evaporator, adding ethanol into a concentrated solution, performing dissolving overnight, and conducting filtering through a centrifugal machine to obtain a precipitate which is crude polysaccharide; and deproteinization treatment of the crude polysaccharide via a Sevag method: putting a crude polysaccharide solution into a separating funnel, adding a Sevag reagent, conducting oscillating and centrifuging to obtain a supernatant, carrying out dialyzing with distilled water to obtain a solvent, adding hydrogen peroxide, carrying out heat preservation and decolorization, conducting concentrating again, and carrying out vacuum freeze-drying to obtain a pure polysaccharide product. The total content of the burdock tea polysaccharide purified by the method is increased by 1.5 times compared with burdock roots serving as a raw material. The burdock tea polysaccharide has good in-vitro antioxidant activity, so the economic value of the burdock tea is improved, and good market development prospects are obtained.

The invention relates to anti-wrinkle eye cream. The anti-wrinkle eye cream is prepared from, by mass, 2-9% of plant oil, 13-32% of moisturizing agent, 1-5% of water-soluble fullerene, 0.1-5% of selenium-rich black fungus selenium polysaccharide, 0.1-5% of lotus leaf polysaccharide, 0.5-3% of emulgator, 0.1-3% of thickening agent and 0.1-0.5% of conditioner. The adopted water-soluble fullerene has very good oxidation resistance performance, can effectively remove activated oxygen free radicals, improve microcirculation, activate skin cells and prevent senility. The components in the composition have a synergistic interaction effect, and the anti-wrinkle eye cream has the effects of remarkably fading eye wrinkles, improving black eyes and under-eye puffiness, deeply moisturizing the skin and the like and makes the eye skin full of energy.

The invention discloses a preparation method of nano-selenium mussel powder, and belongs to the technical field of aquatic product processing. The method comprises the following steps of pre-processing of mussels, co-extracting of mussel protein and polysaccharides and nano-selenizing of a mussel protein and polysaccharide compound. According to the preparation method of the nano-selenium mussel powder, the protein and polysaccharide co-extracting technology is adopted, the mussel protein and polysaccharide compound extract is obtained, and the antioxidant activity in vitro is greatly increased through the synergistic effect of the two ingredients. The mussel polysaccharide and protein peptides act synergistically and jointly serve as templates to regulate and control aggregation of elemental selenium, the regulation and control capacity is enhanced, the particle size of selenium is smaller and more uniform, the antioxidant activity in vitro is greatly increased, the concentration of the templates of a reaction system is higher, and the production efficiency of subsequent concentrating and drying is improved.

The invention discloses a method for extracting auricularia auricular polysaccharide by using a biological enzyme method, which comprises the following steps: adding auricularia auricular powder intodeionized water, adding cellulase and papain, and extracting for 1-5h at 45-80 DEG C under the condition that the pH value is 4.0-8.0; adding one of beta glucanase or mannase or neutral protease, andextracting for 1-5h under the conditions that the temperature is 45-80 DEG C and the pH value is 2.0-9.0; then heating to deactivate enzyme, centrifuging, taking supernatant, adding 95% ethanol, standing for 10 hours at 4 DEG C, and centrifuging; taking the precipitate, dissolving the precipitate in deionized water, deproteinizing by a sevag method until the upper layer has no absorption peak at 280nm, concentrating, and freeze-drying to obtain the auricularia auricular polysaccharide. The extraction yield of the auricularia auricula polysaccharide reaches 36.43%, the removal rate of ABTS < +> is 98.32%, the removal rate of DPPH free radicals is 92.74%, and the removal rate of hydrogen peroxide is 66.56%.

The invention relates to a naringenin acylhydrazone derivative with good antioxidant activity and a preparation method thereof. The preparation method specifically comprises the following steps of: taking and dissolving 0.8-1.2 mmol of naringenin and 0.8-1.2 mmol of a hydrazide compound in 18-22 ml of ethanol, adding 1.8-2.2 ml of acetic acid, heating in a 700 W microwave oven for 3-5 min, and synthesizing to obtain the naringenin acylhydrazone derivative. The preparation method has the advantages that the preparation is convenient, the in-vitro antioxidant activity and effect of the obtained derivative are good, the antioxidant activity of the derivative is equivalent to that of an existing food additive, and the derivative can be further studied as an anti-inflammatory and anti-cancer drug.

The invention provides a fructus sophorae total flavonoid extract with broad-spectrum anti-tumor activity and a preparation method and application of the fructus sophorae total flavonoid extract. The fructus sophorae total flavonoid extract at least comprises quercetin, genistein, kaempferol and isorhamnetin according to the mass ratio of (2-3): (14-16): (15-17): (0.5-1), and has a favorable in-vitro anti-oxidative function, broad-spectrum anti-tumor activity, and a remarkable inhibiting effect on human esophagus cancer, liver cancer, breast cancer, human lymphoma and the like. The preparation method comprises the following steps: taking nut parts of fructus sophorae in autumn as the raw materials; then taking ethanol of 80 percent, 60 percent and 40 percent volume fractions as solvents for gradient ultrasonic extraction, macroporous adsorption resin adsorption and elution; collecting the eluant; concentrating and drying. The preparation method remarkably improves the extraction ratio of fructus sophorae total flavonoids, simplifies the extraction steps and reduces impurity interference.

The invention discloses an extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing the extraction method, and belongs to the technical field of total flavone extraction. According to the extraction method, the extraction rate is taken as a main inspection index, factors of an extraction agent, a solid-liquid ratio, extraction time, the extraction temperature and the like are considered, an extraction method for the total flavone of the potentilla anserine L. through microwave-assisted extraction is optimized, and the anti-oxygenation effect and components of the flavone of the potentilla anserine L. are studied primarily; the technology process is simple, the requirements for the production equipment and technology are low, the extraction cost is low, and the extraction efficiency of the total flavone is high; higher popularization and application value is achieved, and the flavone of the potentilla anserine L. has good in-vitro antioxidant activity and is a novel natural antioxidant or a functional food.

The invention specifically provides a method for completely degrading enteromorpha polysaccharide by virtue of a bio-enzyme. The method comprises the following steps: preparing crude polysaccharide into a 5mg / mL crude polysaccharide water solution; conducting a reaction at certain pH and temperature for a certain duration; boiling enzymatic hydrolysate, which is obtained after enzymatic hydrolysis, for 5min; conducting centrifuging and removing the enzyme; regulating supernatant liquid until pH value is neutral by virtue of alkali; then, dialyzing an obtained solution by virtue of a dialysis bag which is 500 in molecular weight cutoff for 24h for removing inorganic salt; and freeze-drying dialysate, so that the enzymatically degraded enteromorpha polysaccharide is obtained, wherein the molecular weight of the enteromorpha polysaccharide is 1.9645*10<3> Da and a degrading rate on the enteromorpha polysaccharide is 100%. Obtained oligosaccharide, which is low in molecular weight, is basically identical to the enteromorpha polysaccharide in chemical structure, but antioxidant activity in vitro of the oligosaccharide is obviously improved.

The invention provides a preparation method of a succinylated tobacco leaf polysaccharide. The preparation method comprises the following steps of extraction of a tobacco leaf polysaccharide, refiningof the tobacco leaf polysaccharide and succinylation treatment of the tobacco leaf polysaccharide, wherein the succinylation treatment of the tobacco leaf polysaccharide comprises the following stepsof adding anhydrous sodium carbonate and succinic anhydride into an aqueous solution of the refined tobacco leaf polysaccharide, adjusting the pH to 9.8 to 10.2 after the completion of an acylation reaction, and then carrying out solid-liquid phase separation; and regulating the pH of the separated supernatant to neutral, and carrying out dialysis and freeze drying. The preparation method of thesuccinylated tobacco leaf polysaccharide provided by the invention is simple, and is high in the succinylation substitution degree. When being applied to the moisture preservation of cut tobacco, thesuccinylated tobacco leaf polysaccharide provided by the invention has relatively slow water loss rate under a low humidity condition, has relatively slow water absorption rate under a high humidity environment, and has relatively high moisture preservation and moisture-proof function. Moreover, the succinylated tobacco leaf polysaccharide provided by the invention has higher in-vitro antioxidantactivity compared with an unmodified tobacco leaf polysaccharide.

The invention relates to the technical field of food microorganisms, in particular to lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver injury and application of the lactobacillus helveticus ZJUIDS12. The invention discloses lactobacillus helveticus ZJUIDS12, and the preservation number of the lactobacillus helveticus ZJUIDS12 is CGMCC (China General Microbiological Culture Collection Center) NO.23997. The lactobacillus helveticus ZJUIDS12 can be used for preparing the lactobacillus helveticus. The invention also discloses an application of the lactobacillus helveticus ZJUIDS12 in the preparation of a product for protecting the liver injury at the same time, and the lactobacillus helveticus ZJUIDS12 can be used for preparing the product for protecting the liver injury at the same time.

The invention discloses a method for extracting a purified polyphenol compound from rhizoma polygonati. The method comprises the following steps: crushing the rhizoma polygonati into grains with the size of 40 meshes and putting the rhizoma polygonati grains into a water solution containing sodium nitrate; immersing for 10 to 15min and drying; adding an ethanol solution which is 25 to 30 times ofthe weight of and carrying out flash type extraction to obtain a rhizoma polygonati polyphenol crude extraction solution; carrying out elution and purification on the rhizoma polygonati polyphenol crude extraction solution by adopting macroporous resin to obtain purified rhizoma polygonati polyphenol. According to the method disclosed by the invention, the rhizoma polygonati is pre-treated, the degradation of the polyphenol compound in the rhizoma polygonati in a flash extraction process is effectively reduced and the extraction rate of the rhizoma polygonati polyphenol is improved; the rhizoma polygonati polyphenol crude extraction solution is eluted and purified by adopting the macroporous resin and the purity of the rhizoma polygonati polyphenol is improved to 94.2 percent or more; a foundation is laid for obtaining a polyphenol compound monomer; meanwhile, the in-vitro antioxidant activity of rhizoma polygonati polyphenol extract is also remarkably improved; a rhizoma polygonati polyphenol compound is further widely utilized.

The invention discloses a method for preparing and purifying grifola frondosa polysaccharide with high in-vitro antioxidant activity. According to the method, farnesol is added at beginning of or in a process of liquid fermentation of grifola frondosa. According to the method, farnesol is added in the liquid fermentation process of grifola frondosa mycelia, so that polysaccharide yield of grifola frondosa fermentation liquor is promoted to be changed, yield of acidic polysaccharide components generated by grifola frondosa fermentation is remarkably increased, the polysaccharide yield of the grifola frondosa fermentation liquor is remarkably increased on the basis of not increasing an original fermentation period, the maximum increase amplitude reaches 149%, content of the separated and purified acidic polysaccharide is increased from 7.4% to 58.2%, the in-vitro antioxidant activity of the separated and purified acidic polysaccharide is remarkably improved compared with that of a control group, and the exogenous additive farnesol is safe, non-toxic and high in stability, so that a foundation is laid for industrial production of polysaccharide in the grifola frondosa fermentation liquor.

The invention provides an active part of a radish leaf and application of the active part. The active part is prepared by the following steps: firstly, drying the radish leaf, crushing and enabling the crushed substance to pass through a sieve with 200 meshes; secondly, adding the sieved radish leaf into an aqueous solution of ethanol for leaching at constant temperature; performing suction filtering, repeatedly extracting filter residues for three times, combining filtrate, concentrating under reduced pressure and recovering the ethanol, thus obtaining an ethanol extract of the radish leaf; thirdly, performing ultrasonic dispersion on the ethanol extract of the radish leaf by using water, then performing fractional extraction by sequentially using petroleum ether, chloroform, ethyl acetate and water-saturated normal butanol, respectively extracting for three times, and combining the same extracting solutions; concentrating a combined solvent under reduced pressure, and performing freeze drying to respectively obtain a petroleum ether part, a chloroform part, an ethyl acetate part, a water-saturated normal butanol part and a water part, wherein the ethyl acetate part is the active part of the radish leaf. The prepared active part of the radish leaf has the effect of prolonging the service life of drosophila melanogaster, a good antioxidation effect and potential of delaying senescence.

The invention relates to the field of polysaccharide separation and purification methods and application, in particular to an acidic brasenia schreberi polysaccharide and a separation and purification method and application thereof. The method comprises the following steps: firstly preparing crude polysaccharide, then deproteinizing, separating by adopting a DEAE-52 cellulose column to obtain the high-purity acidic brasenia schreberi polysaccharide BSP-5, and carrying out structure identification on the polysaccharide by virtue of methods such as infrared spectroscopic analysis, ultraviolet full-wavelength scanning, high performance liquid chromatography monosaccharide composition analysis, gel permeation chromatography molecular weight determination and the like. The results show that the polysaccharide is alpha-configuration pyranose with uniform properties, is composed of D-mannose, D-galactose, D-xylose and L-fucose in a molar ratio of 0.08: 1.39: 1: 0.23, is an acidic heteropolysaccharide, and has an average molecular weight of 119.5 KD. In-vitro anti-oxidation and anti-tumor experiment results show that the separated and purified acid brasenia schreberi polysaccharide has relatively strong anti-oxidation effect and anti-tumor biological activity.

The invention provides a method for improving biological activity of a porphyra yezoensis polysaccharide. The method comprises the following steps that hydrogen peroxide and ascorbic acid are added into a crude polysaccharide solution and diluted with water to obtain a mixed solution; the obtained mixed solution is subjected to a heat preservation stirring reaction for 1-3 hours at 40-60 DEG C toobtain a reaction liquid; the obtained reaction liquid is concentrated to 15%-20% of the original volume to obtain a concentrated liquid; anhydrous ethanol is added into the concentrated liquid, standing is carried out at 0-5 DEG C overnight, then centrifugation is carried out, and a precipitate is obtained; the obtained precipitate is mixed with deionized water to be dissolved again and dialyzedto obtain a dialyzate I; the obtained dialyzate I is concentrated and then freeze-dried to obtain a porphyra yezoensis degraded polysaccharide. Compared with a porphyra yezoensis crude polysaccharide,the porphyra yezoensis degraded polysaccharide has the advantages that the antioxidant activity in vitro, bile acid binding activity and immunocompetence are all obviously improved. The invention also provides the application of the degraded porphyra yezoensis polysaccharide as a functional food base material prepared by the method.

The invention provides a preparation method of cationic peptides of sesame pomace (CPSP) and an application of the cationic peptides in the preparation of functional foods. The preparation method of the CPSP comprises the steps of smashing sesame pomace, adding a protein extracting solution for extracting, adding ammonium sulfate precipitation peptides, and performing desalting, anion exchange chromatography and the like to obtain the pure CPSP. The CPSP have good antioxidant activity, can significantly prolong the healthy life of caenorhabditis elegans, and can be applied to the anti-aging functional foods serving as functional factors.

The present invention discloses an enteromorpha polysaccharide biological activity improving method comprising the following steps: enteromorpha polysaccharide is degraded by hydrogen peroxide-vitamin C combined method to obtain degraded enteromorpha polysaccharide (DEP); chloroacetic acid as a carboxymethylation reagent is reacted with the degraded enteromorpha polysaccharide (DEP) to prepare carboxymethylated enteromorpha polysaccharide (CDEP); and in the presence of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), the CDEP is coupled with hydroxylamine to obtain hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP). The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has a relatively low molecular weight (30-13kDa), and iron-chelating capacity is 0.2-1.2mmol / g. The hydroxamic acid acidized degraded enteromorpha polysaccharide (HCDEP) has high in-vitro antioxidant activity and antibacterial activity.

The invention discloses a method for extracting and purifying polyphenolic compounds from Rhizoma Polygonatum, which comprises the following steps: crush Rhizoma Polygonatum into 40-mesh granules, soak in an aqueous solution containing sodium citrate for 10-15 minutes, and dry; add 25-30 The ethanol solution with twice the weight is subjected to flash extraction to obtain the crude Polygonatum polyphenols extract; the crude Polygonatum polyphenols is eluted and purified by using a macroporous resin to obtain purified Polygonatum polyphenols. The present invention effectively reduces the degradation of polyphenols in Polygonatum polyphenols in the flash extraction process through the pretreatment of Polygonatum, improves the extraction rate of Polygonatum polyphenols; Carried out elution and purification, the purity of Polygonatum polyphenols was increased to more than 94.2%, which laid the foundation for the acquisition of polyphenolic compound monomers. At the same time, it also significantly improved the in vitro antioxidant activity of Polygonatum polyphenols extract, which is beneficial to Polygonatum Polyphenols Compounds Further Widely Utilized.

The invention relates to the technical field of food microbes, in particular to Lactobacillus helveticum ZJUIDS12 capable of improving alcoholic liver damage and its application. The invention discloses Lactobacillus helveticus ZJUIDS12, and its preservation number is CGMCC NO.23997. The invention also simultaneously discloses the application of the Lactobacillus helveticus ZJUIDS12 in the preparation of a product for protecting liver damage.

The invention provides a preparation method of succinylated tobacco leaf polysaccharide, which comprises the following steps: extraction of tobacco leaf polysaccharide, refining of tobacco leaf polysaccharide, and succinylation of tobacco leaf polysaccharide; the succinylation of tobacco leaf polysaccharide is carried out according to the following process : add anhydrous sodium carbonate, succinic anhydride to the aqueous solution of the tobacco leaf polysaccharide after described refining, adjust pH 9.8~10.2 after the acylation reaction is completed, carry out solid-liquid phase separation afterwards; Get the supernatant after separating and adjust pH to Neutral, dialyzed, freeze-dried. The preparation method of a succinylated tobacco leaf polysaccharide of the present invention is simple, and the degree of succinylation substitution is high; the succinylated tobacco leaf polysaccharide of the present invention is applied to the moisture retention of shredded tobacco, and has relatively slow dehydration under low humidity conditions. The water loss rate has a slower water absorption rate in a high-humidity environment, and has better moisture retention and moisture-proof functions; and the succinylated tobacco leaf polysaccharide of the present invention has better in vitro Antioxidant activity.

The invention discloses a method for preparing peanut antioxidant peptide through ultrasonic-assisted enzymolysis. The method comprises the following steps of: adding petroleum ether into peanut cake meal, leaching and degreasing at constant temperature, and drying to obtain degreased peanut cake meal; adding distilled water into the degreased peanut cake meal, and dissolving by using ultrasonic wave to obtain peanut cake meal suspension; regulating the pH value of the peanut cake meal suspension by using solution of hydrochloric acid, adding composite plant hydrolase ViscozymeL, performing ultrasonic-assisted enzymolysis to obtain enzymatic solution, deactivating enzyme, and cooling to room temperature; and regulating the pH value of the enzymatic solution by using solution of sodium hydroxide, adding protease, performing ultrasonic-assisted enzymolysis, deactivating enzyme, cooling to room temperature, centrifuging, and freeze-drying to obtain the peanut antioxidant peptide. In the method, the ultrasonic-assisted enzymolysis is utilized, so that the release amount of peptide in the enzymolysis process can be improved, the antioxidant activity of the obtained peanut antioxidant peptide is strengthened, and a theoretical foundation is provided for the development and application of peanut cake meal proteins.

The invention discloses a method for enhancing the bioactivity of hijiki polysaccharides, which comprises the following steps: the hijiki crude polysaccharides are placed in a buffer solution with a pH of 4.5 to 7.0 and acted on by a compound enzyme composed of pectinase and glucoamylase The degradation reaction was carried out under the following conditions; the obtained reaction solution was filtered after removing enzymes, and the obtained filtrate was concentrated and then dialyzed with a dialysis bag with a molecular weight cut-off of 3500 Da. Adopting the method of the invention can not only significantly improve the antioxidant activity and bile acid binding ability of the hijiki polysaccharide, but also enhance its immune regulation activity on macrophages.

The invention provides a preparation method of a skipjack oxidation resistant compound. The pH value of skipjack fresh slurry is adjusted to 7.0 to 8.0, after enzymolysis is conducted, enzymatic hydrolysate is centrifuged and filtered twice, and then the skipjack oxidation resistant compound is obtained. According to the two times of filtration, a ceramic membrane is used for filtration at the first time, and a nanofiltration membrane is used for filtration at the second time. The peptide constituent below 1000 Daltons in the skipjack oxidation resistant compound accounts for over 95% and is easy to absorb and digest; the reducing power is equivalent to that of 0.125 mg / mL of glutathione when the concentration is 5 mg / mL; and the IC50 value of scavenging activity of a 1,1-diphenyl-picrylhydrazyl free radical is 2.68 mg / mL, the IC50 value of scavenging activity of a superoxide anion free radical is 16.14 mg / mL, and the IC50 value of scavenging activity of a hydroxyl free radical is 21.04 mg / mL. The preparation process is simple in technology, and industrialization is easy. The product passes in vitro experiments and in vivo experiments, it is shown that the product has the good oxidation resistance effect, and the good development and use prospects are shown.