47results about How to "Good in vitro antioxidant activity" patented technology

The invention relates to an anti-oxidation mask, which comprises the following components in mass percentage: moisturizing agent: 10-25%, fullerene: 0.1-3%, quinoa extract: 0.1-5%, Alihong extract: 0.1- 5%, Thickener: 0.1‑3%, Conditioner: 0.1‑0.5%. The fullerene used in the present invention has good antioxidant properties, can effectively remove active oxygen free radicals, activate skin cells, and prevent aging; the quinoa extract is rich in polyphenols and flavonoids, and has good antioxidant properties in vitro Sex; Alihong extract contains chemical components such as flavonoids, polysaccharides, saponins and terpenes, which can improve the skin's immunity and play an anti-oxidation and anti-aging effect; there is a synergistic effect between the components in the formula, It has moisturizing, anti-oxidation and anti-aging effects.

The invention particularly relates to a method for extracting hovenia dulcis thunb total flavones by cooperation of a surfactant and a microwave-ultrasonic extraction process. The method comprises the following steps: (1) grinding hovenia dulcis thunb, and performing Soxhlet extraction by petroleum ether to remove fat so as to obtain fully defatted ground hovenia dulcis thunb; (2) uniformly mixing the ground hovenia dulcis thunb, distilled water and the surfactant to obtain a mixed solution; (3) performing microwave-ultrasonic extraction on the mixed solution at the microwave power of 200-600 W and the ultrasonic power of 100-500 W for 5-20 minutes; (4) after the extraction, performing alcohol precipitation on the extracted filtrate to remove alcohol supernatant, and concentrating and drying to obtain a hovenia dulcis thunb total flavones extract. Due to the adoption of the method for extracting hovenia dulcis thunb total flavones, the extraction time is greatly shortened, the extraction efficiency is improved, the cost is reduced, and the energy consumption is low; meanwhile, a distilled water solution in which a small amount of the surfactant is dissolved is taken as an extraction solution, so that the problem of toxic organic solvent residues is solved.

The cigarette with super low free radical content features its delayed released plant polyphenol microcapsule content of 0.02-0.6 wt% of shredded tobacco. The plant polyphenol microcapsule is first dissolved in water, alcohol, mixture of water and alcohol or water solution of propylene glycol and the solution is then sprayed to shredded tobacco. The delayed released plant polyphenol microcapsule may be catechin microcapsule, gallic acid microcapsule as degraded product of tannin or grape polyphenol microcapsule. The added plant polyphenol has stability raised by 9-12 times, extraneous antioxidant performance raised by 5-6 times and intracorporal antioxidant performance raised by about 10 times. Its effective component may be absorbed by oral cavity and respiratory system to eliminate free radical in body while meeting the need of smokers.

The invention belongs to the technical field of food biology, and particularly relates to an ultrasound-assisted extraction method of flavonoid compounds in citrus peel as well as research on the flavonoid compounds. The ultrasound-assisted extraction method of flavonoid compounds in citrus peel disclosed by the invention comprises the following steps: drying fresh citrus peel, and carrying out crushing to obtain citrus peel powder; adding an ethanol solution into the citrus peel powder, and performing ultrasonic extraction to obtain a flavone crude extract; and then, purifying the flavonoid compounds in the citrus peels by using macroporous resin to obtain a citrus peel extract containing the flavonoid compounds. The citrus peel extract containing the flavonoid compounds has high in-vitro antioxidant activity, and is capable of efficiently reducing blood glucose and blood fat; and moreover, the citrus peel extract can be efficiently fermented with intestinal flora to improve diversity of intestinal microorganisms.

The invention discloses an extraction and purification method of burdock tea polysaccharide. The method comprises the following main steps: extraction of polysaccharide by using a water extraction andalcohol precipitation method: weighing prepared burdock tea, pulverizing the burdock tea, putting the pulverized burdock tea into a container, adding water, carrying out uniform mixing, conducting concentrating by using a rotary evaporator, adding ethanol into a concentrated solution, performing dissolving overnight, and conducting filtering through a centrifugal machine to obtain a precipitate which is crude polysaccharide; and deproteinization treatment of the crude polysaccharide via a Sevag method: putting a crude polysaccharide solution into a separating funnel, adding a Sevag reagent, conducting oscillating and centrifuging to obtain a supernatant, carrying out dialyzing with distilled water to obtain a solvent, adding hydrogen peroxide, carrying out heat preservation and decolorization, conducting concentrating again, and carrying out vacuum freeze-drying to obtain a pure polysaccharide product. The total content of the burdock tea polysaccharide purified by the method is increased by 1.5 times compared with burdock roots serving as a raw material. The burdock tea polysaccharide has good in-vitro antioxidant activity, so the economic value of the burdock tea is improved, and good market development prospects are obtained.

The invention discloses a preparation method of nano-selenium mussel powder, and belongs to the technical field of aquatic product processing. The method comprises the following steps of pre-processing of mussels, co-extracting of mussel protein and polysaccharides and nano-selenizing of a mussel protein and polysaccharide compound. According to the preparation method of the nano-selenium mussel powder, the protein and polysaccharide co-extracting technology is adopted, the mussel protein and polysaccharide compound extract is obtained, and the antioxidant activity in vitro is greatly increased through the synergistic effect of the two ingredients. The mussel polysaccharide and protein peptides act synergistically and jointly serve as templates to regulate and control aggregation of elemental selenium, the regulation and control capacity is enhanced, the particle size of selenium is smaller and more uniform, the antioxidant activity in vitro is greatly increased, the concentration of the templates of a reaction system is higher, and the production efficiency of subsequent concentrating and drying is improved.

The invention discloses a method for extracting auricularia auricular polysaccharide by using a biological enzyme method, which comprises the following steps: adding auricularia auricular powder intodeionized water, adding cellulase and papain, and extracting for 1-5h at 45-80 DEG C under the condition that the pH value is 4.0-8.0; adding one of beta glucanase or mannase or neutral protease, andextracting for 1-5h under the conditions that the temperature is 45-80 DEG C and the pH value is 2.0-9.0; then heating to deactivate enzyme, centrifuging, taking supernatant, adding 95% ethanol, standing for 10 hours at 4 DEG C, and centrifuging; taking the precipitate, dissolving the precipitate in deionized water, deproteinizing by a sevag method until the upper layer has no absorption peak at 280nm, concentrating, and freeze-drying to obtain the auricularia auricular polysaccharide. The extraction yield of the auricularia auricula polysaccharide reaches 36.43%, the removal rate of ABTS < +> is 98.32%, the removal rate of DPPH free radicals is 92.74%, and the removal rate of hydrogen peroxide is 66.56%.

The invention relates to a naringenin acylhydrazone derivative with good antioxidant activity and a preparation method thereof. The preparation method specifically comprises the following steps of: taking and dissolving 0.8-1.2 mmol of naringenin and 0.8-1.2 mmol of a hydrazide compound in 18-22 ml of ethanol, adding 1.8-2.2 ml of acetic acid, heating in a 700 W microwave oven for 3-5 min, and synthesizing to obtain the naringenin acylhydrazone derivative. The preparation method has the advantages that the preparation is convenient, the in-vitro antioxidant activity and effect of the obtained derivative are good, the antioxidant activity of the derivative is equivalent to that of an existing food additive, and the derivative can be further studied as an anti-inflammatory and anti-cancer drug.

The invention discloses an extraction method for total flavone of potentilla anserine L. and extraction equipment utilizing the extraction method, and belongs to the technical field of total flavone extraction. According to the extraction method, the extraction rate is taken as a main inspection index, factors of an extraction agent, a solid-liquid ratio, extraction time, the extraction temperature and the like are considered, an extraction method for the total flavone of the potentilla anserine L. through microwave-assisted extraction is optimized, and the anti-oxygenation effect and components of the flavone of the potentilla anserine L. are studied primarily; the technology process is simple, the requirements for the production equipment and technology are low, the extraction cost is low, and the extraction efficiency of the total flavone is high; higher popularization and application value is achieved, and the flavone of the potentilla anserine L. has good in-vitro antioxidant activity and is a novel natural antioxidant or a functional food.

The invention specifically provides a method for completely degrading enteromorpha polysaccharide by virtue of a bio-enzyme. The method comprises the following steps: preparing crude polysaccharide into a 5mg/mL crude polysaccharide water solution; conducting a reaction at certain pH and temperature for a certain duration; boiling enzymatic hydrolysate, which is obtained after enzymatic hydrolysis, for 5min; conducting centrifuging and removing the enzyme; regulating supernatant liquid until pH value is neutral by virtue of alkali; then, dialyzing an obtained solution by virtue of a dialysis bag which is 500 in molecular weight cutoff for 24h for removing inorganic salt; and freeze-drying dialysate, so that the enzymatically degraded enteromorpha polysaccharide is obtained, wherein the molecular weight of the enteromorpha polysaccharide is 1.9645*10<3> Da and a degrading rate on the enteromorpha polysaccharide is 100%. Obtained oligosaccharide, which is low in molecular weight, is basically identical to the enteromorpha polysaccharide in chemical structure, but antioxidant activity in vitro of the oligosaccharide is obviously improved.

The invention provides a preparation method of a succinylated tobacco leaf polysaccharide. The preparation method comprises the following steps of extraction of a tobacco leaf polysaccharide, refiningof the tobacco leaf polysaccharide and succinylation treatment of the tobacco leaf polysaccharide, wherein the succinylation treatment of the tobacco leaf polysaccharide comprises the following stepsof adding anhydrous sodium carbonate and succinic anhydride into an aqueous solution of the refined tobacco leaf polysaccharide, adjusting the pH to 9.8 to 10.2 after the completion of an acylation reaction, and then carrying out solid-liquid phase separation; and regulating the pH of the separated supernatant to neutral, and carrying out dialysis and freeze drying. The preparation method of thesuccinylated tobacco leaf polysaccharide provided by the invention is simple, and is high in the succinylation substitution degree. When being applied to the moisture preservation of cut tobacco, thesuccinylated tobacco leaf polysaccharide provided by the invention has relatively slow water loss rate under a low humidity condition, has relatively slow water absorption rate under a high humidity environment, and has relatively high moisture preservation and moisture-proof function. Moreover, the succinylated tobacco leaf polysaccharide provided by the invention has higher in-vitro antioxidantactivity compared with an unmodified tobacco leaf polysaccharide.

The invention relates to the technical field of food microorganisms, in particular to lactobacillus helveticus ZJUIDS12 capable of improving alcoholic liver injury and application of the lactobacillus helveticus ZJUIDS12. The invention discloses lactobacillus helveticus ZJUIDS12, and the preservation number of the lactobacillus helveticus ZJUIDS12 is CGMCC (China General Microbiological Culture Collection Center) NO.23997. The lactobacillus helveticus ZJUIDS12 can be used for preparing the lactobacillus helveticus. The invention also discloses an application of the lactobacillus helveticus ZJUIDS12 in the preparation of a product for protecting the liver injury at the same time, and the lactobacillus helveticus ZJUIDS12 can be used for preparing the product for protecting the liver injury at the same time.

The invention discloses a method for preparing and purifying grifola frondosa polysaccharide with high in-vitro antioxidant activity. According to the method, farnesol is added at beginning of or in a process of liquid fermentation of grifola frondosa. According to the method, farnesol is added in the liquid fermentation process of grifola frondosa mycelia, so that polysaccharide yield of grifola frondosa fermentation liquor is promoted to be changed, yield of acidic polysaccharide components generated by grifola frondosa fermentation is remarkably increased, the polysaccharide yield of the grifola frondosa fermentation liquor is remarkably increased on the basis of not increasing an original fermentation period, the maximum increase amplitude reaches 149%, content of the separated and purified acidic polysaccharide is increased from 7.4% to 58.2%, the in-vitro antioxidant activity of the separated and purified acidic polysaccharide is remarkably improved compared with that of a control group, and the exogenous additive farnesol is safe, non-toxic and high in stability, so that a foundation is laid for industrial production of polysaccharide in the grifola frondosa fermentation liquor.

The invention provides an active part of a radish leaf and application of the active part. The active part is prepared by the following steps: firstly, drying the radish leaf, crushing and enabling the crushed substance to pass through a sieve with 200 meshes; secondly, adding the sieved radish leaf into an aqueous solution of ethanol for leaching at constant temperature; performing suction filtering, repeatedly extracting filter residues for three times, combining filtrate, concentrating under reduced pressure and recovering the ethanol, thus obtaining an ethanol extract of the radish leaf; thirdly, performing ultrasonic dispersion on the ethanol extract of the radish leaf by using water, then performing fractional extraction by sequentially using petroleum ether, chloroform, ethyl acetate and water-saturated normal butanol, respectively extracting for three times, and combining the same extracting solutions; concentrating a combined solvent under reduced pressure, and performing freeze drying to respectively obtain a petroleum ether part, a chloroform part, an ethyl acetate part, a water-saturated normal butanol part and a water part, wherein the ethyl acetate part is the active part of the radish leaf. The prepared active part of the radish leaf has the effect of prolonging the service life of drosophila melanogaster, a good antioxidation effect and potential of delaying senescence.

The invention relates to the field of polysaccharide separation and purification methods and application, in particular to an acidic brasenia schreberi polysaccharide and a separation and purification method and application thereof. The method comprises the following steps: firstly preparing crude polysaccharide, then deproteinizing, separating by adopting a DEAE-52 cellulose column to obtain the high-purity acidic brasenia schreberi polysaccharide BSP-5, and carrying out structure identification on the polysaccharide by virtue of methods such as infrared spectroscopic analysis, ultraviolet full-wavelength scanning, high performance liquid chromatography monosaccharide composition analysis, gel permeation chromatography molecular weight determination and the like. The results show that the polysaccharide is alpha-configuration pyranose with uniform properties, is composed of D-mannose, D-galactose, D-xylose and L-fucose in a molar ratio of 0.08: 1.39: 1: 0.23, is an acidic heteropolysaccharide, and has an average molecular weight of 119.5 KD. In-vitro anti-oxidation and anti-tumor experiment results show that the separated and purified acid brasenia schreberi polysaccharide has relatively strong anti-oxidation effect and anti-tumor biological activity.

The invention provides a method for improving biological activity of a porphyra yezoensis polysaccharide. The method comprises the following steps that hydrogen peroxide and ascorbic acid are added into a crude polysaccharide solution and diluted with water to obtain a mixed solution; the obtained mixed solution is subjected to a heat preservation stirring reaction for 1-3 hours at 40-60 DEG C toobtain a reaction liquid; the obtained reaction liquid is concentrated to 15%-20% of the original volume to obtain a concentrated liquid; anhydrous ethanol is added into the concentrated liquid, standing is carried out at 0-5 DEG C overnight, then centrifugation is carried out, and a precipitate is obtained; the obtained precipitate is mixed with deionized water to be dissolved again and dialyzedto obtain a dialyzate I; the obtained dialyzate I is concentrated and then freeze-dried to obtain a porphyra yezoensis degraded polysaccharide. Compared with a porphyra yezoensis crude polysaccharide,the porphyra yezoensis degraded polysaccharide has the advantages that the antioxidant activity in vitro, bile acid binding activity and immunocompetence are all obviously improved. The invention also provides the application of the degraded porphyra yezoensis polysaccharide as a functional food base material prepared by the method.

The invention provides a preparation method of cationic peptides of sesame pomace (CPSP) and an application of the cationic peptides in the preparation of functional foods. The preparation method of the CPSP comprises the steps of smashing sesame pomace, adding a protein extracting solution for extracting, adding ammonium sulfate precipitation peptides, and performing desalting, anion exchange chromatography and the like to obtain the pure CPSP. The CPSP have good antioxidant activity, can significantly prolong the healthy life of caenorhabditis elegans, and can be applied to the anti-aging functional foods serving as functional factors.

The invention provides a raspberry/sea buckthorn/nitraria tangutorum composite polysaccharide composition, which is composed of, by weight, 3-18 parts of raspberry polysaccharide, 4-16 parts of sea buckthorn polysaccharide, and 3-20 parts of nitraria tangutorum polysaccharide. The invention also discloses an application of the composition. Researching result proves that the raspberry polysaccharide, sea buckthorn polysaccharide and nitraria tangutorum polysaccharide have excellent in-vitro anti-oxidizing activity and can significantly remove hydroxyl free radical, superoxide anion free radicaland ABTS free radical. The three polysaccharides are combined so that the composition is significantly improved in in-vitro anti-oxidizing activity, which proves that the three polysaccharides have synergistic effect.