Extraction and purification method of burdock tea polysaccharide

A purification method and technology of tea polysaccharides, applied in the field of extraction and purification of burdock tea polysaccharides, can solve the problems of lack of economic value and low extraction efficiency of burdock tea polysaccharides, and achieve economic value improvement, good in vitro antioxidant activity, and good market development foreground effect

Pending Publication Date: 2020-08-21
华容县胜峰茶业有限公司
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AI Technical Summary

Problems solved by technology

However, there is currently no polysaccharide purification method for burdock on the market. The traditional puri

Method used

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Examples

Experimental program
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Example Embodiment

[0008] Example

[0009] Weigh the prepared burdock tea into a beaker after crushing it, add ten times the quality of the tea powder and mix it evenly, in a water bath at 65℃ for 2.5h, use a rotary evaporator, set the speed to 70r / min, bath temperature 65℃, time 25min , Concentrated to 0.2 times the original volume. Add 5 times the volume of 95% ethanol to the concentrated solution and dissolve for 12 hours. Centrifuge at 5000r / min for 10min and filter the precipitate to obtain crude polysaccharide. The crude polysaccharide was deproteinized by the Sevag method. The volume ratio of chloroform-n-butanol was 4:1 to prepare Sevag reagent. The crude polysaccharide solution was placed in a separatory funnel, and 0.2 times the solution volume of Sevag reagent was added. Shake 18min, 5000r / min Centrifuge for 8 minutes to obtain the supernatant. Continue to repeat the previous operation until no milky white denatured protein is precipitated between the chloroform layer and the n-butanol...

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Abstract

The invention discloses an extraction and purification method of burdock tea polysaccharide. The method comprises the following main steps: extraction of polysaccharide by using a water extraction andalcohol precipitation method: weighing prepared burdock tea, pulverizing the burdock tea, putting the pulverized burdock tea into a container, adding water, carrying out uniform mixing, conducting concentrating by using a rotary evaporator, adding ethanol into a concentrated solution, performing dissolving overnight, and conducting filtering through a centrifugal machine to obtain a precipitate which is crude polysaccharide; and deproteinization treatment of the crude polysaccharide via a Sevag method: putting a crude polysaccharide solution into a separating funnel, adding a Sevag reagent, conducting oscillating and centrifuging to obtain a supernatant, carrying out dialyzing with distilled water to obtain a solvent, adding hydrogen peroxide, carrying out heat preservation and decolorization, conducting concentrating again, and carrying out vacuum freeze-drying to obtain a pure polysaccharide product. The total content of the burdock tea polysaccharide purified by the method is increased by 1.5 times compared with burdock roots serving as a raw material. The burdock tea polysaccharide has good in-vitro antioxidant activity, so the economic value of the burdock tea is improved, and good market development prospects are obtained.

Description

technical field [0001] The invention belongs to the field of polysaccharide purification, and in particular relates to an extraction and purification method of burdock tea polysaccharide. Background technique [0002] Polysaccharide is a substance that exists widely in living organisms. It is a natural polymer, an important macromolecule in living organisms, and one of the basic substances to maintain the normal operation of life activities. Nowadays, the research on plant polysaccharides is attracting more and more attention. International science even proposes that the 21st century is the century of polysaccharides. After scientific verification, many plant polysaccharides have health effects such as regulating immunity, anti-tumor, lowering blood sugar and blood lipids. Pay attention to the research on plant polysaccharides. [0003] Burdock (Arctiumlappa L.), also known as Oriental burdock, belongs to Campanulaceae, Asteraceae biennial herb, mainly distributed in China,...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 杨建昊毛猛
Owner 华容县胜峰茶业有限公司
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