3 results about "Campanulaceae" patented technology

The invention discloses a method for extracting and preparing an oligosaccharide extract from codonopsis pilosula and application of the oligosaccharide extract in prevention and treatment of neurodegenerative disease Alzheimer's disease, and belongs to the technical field of traditional Chinese medicine pharmacy. According to the present invention, the oligosaccharide extract is enriched and prepared from Codonopsis pilosala (Franch.) Nanf., wherein the Codonopsis pilosala (Franch.) Nanf. Is a plant of the family of Platycodon grandiflorum, and the activity test of the oligosaccharide extract on the improvement of cognitive dysfunction in the animal body is performed; results show that in a mouse cognitive function impairment model caused by sleep deprivation, the high-dose codonopsis pilosula oligosaccharide has a prominent effect on improving the learning and memory ability of a Morris water maze and a neuroprotective effect, the efficacy of the codonopsis pilosula oligosaccharide is equivalent to that of a positive control drug manntranet, and no obvious toxic or side effect is found. The codonopsis pilosula oligosaccharide is expected to be further developed into functional food, health care products and / or medicines with the effect of preventing and / or improving cognitive impairment.

The invention discloses a kind of efficient propagation method of Codonopsis pilosula, by using culture medium A in bottle to complete callus induction, then using culture medium B to complete the induction and proliferation steps of adventitious buds, the proliferation coefficient reaches 45.0-50.0;Then cooperate with "micro-cutting" outside the bottle, so that the rooting rate of adventitious buds reaches more than 90%, and subsequent domestication and transplanting steps can be smoothly carried out, and then after continuing to grow and grow, the rooting seedlings with thick roots and strong seedlings are obtained, and the seedling hardening and transplanting are carried out, and the survival rate after transplanting is also more than 90%. The present invention not only simplifies the tissue culture production process of Codonopsis pilosula seedlings, and the proliferation coefficient is much higher than the existing reports of other species of the same family, and the efficient "micro-cutting" rooting system combines adventitious root induction and domestication, greatly shortens the seedling raising cycle of Codonopsis pilosula tissue culture seedlings, solves the low proliferation coefficient in the tissue culture process of Codonopsis pilosula plants in Campanulaceae, the phenomenon such as difficult rooting and domestication, and low cost, short cycle, high quality and survival rate.

The present application relates to the technical field of medicinal plant tissue culture, in particular to a rapid virus-free cloning method and reagent combination for medicinal plant tissue culture. The method comprises the following steps: pretreatment and disinfection of explants, precise virus elimination, preparation of dynamic adaptive medium, rapid propagation, real-time high-throughput detection of virus-free, seedling raising and transplanting, etc. The reagent combination comprises a multifunctional degradable reagent kit, intelligent response type nano disinfection microcapsules, special reagents such as composite virus elimination liquid, etc. The virus elimination rate is greater than or equal to 95%, the survival rate of explants is greater than or equal to 90%, the propagation cycle is shortened by 30%, the detection time is less than or equal to 2 hours, the survival rate of transplanting is greater than or equal to 85%, and the degradation rate of reagents is greater than or equal to 90%. The present application is suitable for herbaceous, woody and liana medicinal plants of the families of Araliaceae, Eucommiaceae, Orchidaceae and Campanulaceae, etc., can realize stable virus elimination and propagation effect, significantly improve the virus-free cloning efficiency and seedling quality, and meet the green production demand.