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Acidic brasenia schreberi polysaccharide as well as separation and purification method and application thereof

A technology for separation, purification and watermelon, which is applied to the field of acid watermelon polysaccharide and its separation and purification, can solve the problems such as the polysaccharide components not being effectively used, and achieve the effect of anti-oxidation and anti-tumor application value.

Pending Publication Date: 2022-03-29
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the technical problem that the polysaccharide components of water shield polysaccharides have not been effectively used in the existing methods for separation and purification of water shield polysaccharides, the present invention provides a method for separation and purification of water shield polysaccharides to obtain a new type of acid water shield polysaccharides, and at the same time study its anti-oxidation in vitro and inhibit the biological activity of tumor cell proliferation

Method used

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  • Acidic brasenia schreberi polysaccharide as well as separation and purification method and application thereof
  • Acidic brasenia schreberi polysaccharide as well as separation and purification method and application thereof
  • Acidic brasenia schreberi polysaccharide as well as separation and purification method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Extraction of Brasenia crude polysaccharide

[0037] 1. Wash the young leaves of fresh water shield with tap water, drain the water, reflux with absolute ethanol to degrease, dry, pulverize with a pulverizer, and pass through a 100-mesh sieve to obtain dry water shield powder;

[0038] 2. Weigh 30g of water shield dry powder, add distilled water to the water shield dry powder, the ratio of solid to liquid is 1:50, adjust the pH value to 4.5, add pectinase with 3% of the substrate mass, control the extraction temperature at 45°C, extract for 3h, 90 Inactivate at ℃ for 10 minutes, filter to remove the dry powder of water shield, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure;

[0039] 3. Add three times the volume of absolute ethanol to the concentrated solution while stirring, let it stand overnight at 4°C, and centrifuge to obtain a brown precipitate;

[0040] 4. The precipitate was fully dissolved in 20mL of distilled w...

Embodiment 2

[0042] Embodiment 2: Extraction of crude polysaccharide of water shield

[0043] 1, take by weighing 20g of the water shield dry powder obtained in Example 1, add distilled water to the water shield dry powder, the ratio of material to liquid is 1:50, adjust the pH value to 5.5, add 2% pectinase of substrate quality, control the extraction temperature Extract at 45°C for 2 hours, inactivate at 90°C for 10 minutes, filter to remove the dry powder of water shield, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure;

[0044] 3. Add three times the volume of absolute ethanol to the concentrated solution while stirring, let it stand overnight at 4°C, and centrifuge to obtain a brown precipitate;

[0045] 4. The precipitate was fully dissolved in 15mL of distilled water, and then freeze-dried to obtain crude water shield polysaccharide;

[0046]5. Fully dissolve the crude water shield polysaccharide in 40mL of distilled water, add 8mL of Savage reagen...

Embodiment 3

[0047] Embodiment 3: Extraction of crude polysaccharide of water shield

[0048] 1, take by weighing 25g of the water shield dry powder obtained in Example 1, add distilled water in the water shield dry powder, the ratio of material to liquid is 1:50, adjust the pH value to 3.5, add the pectinase of 1% of the substrate quality, control the extraction temperature Extract at 45°C for 1 hour, inactivate at 90°C for 10 minutes, filter to remove the dry powder of water shield, and concentrate the filtrate to 1 / 5 of the original volume under reduced pressure;

[0049] 3. Add three times the volume of absolute ethanol to the concentrated solution while stirring, let it stand overnight at 4°C, and centrifuge to obtain a brown precipitate;

[0050] 4. The precipitate was fully dissolved in 10mL of distilled water, and then freeze-dried to obtain crude water shield polysaccharide;

[0051] 5. Fully dissolve the crude water shield polysaccharide in 45mL of distilled water, add 9mL of Sa...

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Abstract

The invention relates to the field of polysaccharide separation and purification methods and application, in particular to an acidic brasenia schreberi polysaccharide and a separation and purification method and application thereof. The method comprises the following steps: firstly preparing crude polysaccharide, then deproteinizing, separating by adopting a DEAE-52 cellulose column to obtain the high-purity acidic brasenia schreberi polysaccharide BSP-5, and carrying out structure identification on the polysaccharide by virtue of methods such as infrared spectroscopic analysis, ultraviolet full-wavelength scanning, high performance liquid chromatography monosaccharide composition analysis, gel permeation chromatography molecular weight determination and the like. The results show that the polysaccharide is alpha-configuration pyranose with uniform properties, is composed of D-mannose, D-galactose, D-xylose and L-fucose in a molar ratio of 0.08: 1.39: 1: 0.23, is an acidic heteropolysaccharide, and has an average molecular weight of 119.5 KD. In-vitro anti-oxidation and anti-tumor experiment results show that the separated and purified acid brasenia schreberi polysaccharide has relatively strong anti-oxidation effect and anti-tumor biological activity.

Description

technical field [0001] The invention relates to a polysaccharide separation and purification method and its application field, in particular to an acidic Brasenia polysaccharide and its separation and purification method and application. Background technique [0002] Polysaccharides are important components ubiquitous in plant cell walls and cells, and their physical and chemical properties and biological activities vary due to their composition and structure. Studies have shown that polysaccharides have various biological activities such as anti-tumor, anti-oxidation and immune regulation. In recent years, the extraction, purification, structural analysis and drug research of new polysaccharides have attracted more and more attention. [0003] Water shield is a perennial aquatic perennial herb, mainly distributed in Jiangxi, Zhejiang, Jiangsu and other places in China. It grows in ponds, lakes and swamps. It is a precious food and medicine dual-purpose plant. Water shiel...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P35/00A61P39/06
CPCC08B37/0003C08B37/006A61K31/715A61P39/06A61P35/00
Inventor 徐德锋祁久乐胡航
Owner CHANGZHOU UNIV
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