The invention relates to a preparation method based on wood frog bone and
flesh collagen polypeptide
chelated calcium. Wood frog bone and
flesh chelated calcium is researched by the aid of a lot of wood frog bone and
flesh, bones are reasonably treated, waste of resources is avoided,
environmental pressure is reduced, and economic and ecological benefits are improved. The method includes the steps: wood frog bone and flesh
powder roughcasting; wood frog bone and flesh
powder enzymolysis; enzymolysis liquid ultra-
filtration; chelating of enzymolysis liquid and
calcium chloride. The preparationmethod has the advantages that polypeptide is prepared by a method of
trypsin enzymolysis, small molecular weight polypeptide is prepared by a nano-
filtration method, and the collagen polypeptide liquid with small molecular weight is acquired. The collagen polypeptide liquid and
calcium ions are chelated by the method to obtain the polypeptide
chelated calcium,
absorption rate in human bodies is increased,
calcium supplement effects are good, the method is simple to operate, chelating can be achieved without freeze-
drying preparation
powder, the method is high in efficiency, easy to control, and prepared collagen polypeptide chelated calcium is high in
chelation rate. The prepared wood frog bone and flesh collagen polypeptide chelated calcium can be used for development of common foods andhealth care products or used for the field of medicines.