Cod steak protein peptide ferrous chelate and preparation method thereof

A protein peptide and chelate technology, applied in the field of functional polypeptide preparation, can solve the problems of non-competitive absorption, not easy to be saturated, not easy to absorb, etc., and achieve the effects of good stability, high chelation rate and low cost

Inactive Publication Date: 2018-04-10
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ferrous amino acid products are more expensive and specific. In addition, ferrous amino acid chelates are easy to polymerize during storage to form compounds that are not easily absorbed by the body. In addition, compared with amino acids, peptides have fast absorption, less saturation, Low energy consumption and no competition with the absorption of amino acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of cod steak protein peptide ferrous chelate: thaw frozen cod steak at 4°C, rinse with tap water, remove residual viscera and fish skin, cook at 121°C for 30 minutes under high temperature and high pressure, and homogenize the homogenate The obtained solids and water were formulated into a cod steak homogenate at a weight ratio of 1:4, and the pH was adjusted to 8.0, and 3000u / g of trypsin was added for enzymolysis at 40°C for 3 hours, and the enzymolysis was terminated in a boiling water bath at 100°C for 10 minutes The reaction results in a cod steak protein peptide solution. Adjust the pH of the cod steak protein peptide solution to 6.0, add ferrous sulfate, the protein peptide: ferrous ion molar ratio of the cod steak protein peptide solution is 4:1, and react at a temperature of 30°C for 20 minutes to obtain the cod steak protein peptide ferrous claw Compound solution, centrifuged at 4000rpm / min for 10min to take the supernatant, added 9 times ...

Embodiment 2

[0027] A preparation method of cod steak protein peptide ferrous chelate: thaw frozen cod steak at 4°C, rinse with tap water, remove residual viscera and fish skin, cook at 121°C for 30 minutes under high temperature and high pressure, and homogenize the homogenate The obtained solids and water were formulated into a cod steak homogenate at a weight ratio of 1:4, and the pH was adjusted to 8.0, and 3000u / g of trypsin was added for enzymolysis at 40°C for 3 hours, and the enzymolysis was terminated in a boiling water bath at 100°C for 10 minutes The reaction results in a cod steak protein peptide solution. Adjust the pH of the cod steak protein peptide solution to 8.0, add ferrous sulfate, the protein peptide: ferrous ion molar ratio is 2:1, react at a temperature of 60°C for 40 minutes, and obtain the cod steak protein peptide ferrous chelate solution, 4000rpm / Centrifuge for 10 min to take the supernatant, add 9 times the volume of absolute ethanol, filter and freeze-dry to o...

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PUM

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Abstract

The invention discloses a preparation method of a cod steak protein peptide ferrous chelate. The preparation method comprises the following steps: (1) enzymatic hydrolysis: cleaning a cod steak, removing residual internal organs and fish skins, boiling the cod steak at a high temperature of 121 DEG C under a high pressure for 30 minutes, homogenizing the cod steak, adding water into solids obtained by homogenizing to form uniform cod slurry, adjusting the pH of the uniform cod slurry to a proper value, adding protease, carrying out sufficient enzymatic hydrolysis, and stopping enzymatic hydrolysis to obtain a cod steak protein peptide solution; (2) chelating: adjusting the pH of the cod steak protein peptide solution, and adding a ferrous salt solution to carry out chelating reactions; and(3) drying: centrifuging the product obtained in the step (2), saving the supernate, and drying the supernate to obtain a finished product. The purity of prepared cod steak protein peptide ferrous chelate is high, the chelating rate between the cod steak protein peptide ferrous chelate and ferrous ions is high, the stability is good, the cod steak protein peptide ferrous chelate can improve the anemia state of newly weaned newborn mice; moreover, the preparation method and technology are simple, the raw materials are easily available, the cost is low, and the preparation method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of preparation of functional polypeptides, and in particular relates to a cod steak protein peptide ferrous chelate and a preparation method thereof. Background technique [0002] Iron is an essential trace element for the human body and is the most abundant in the human body. Iron is widely distributed in the human body and is contained in almost all tissues, with the highest content in the liver and spleen, followed by the kidney, heart, skeletal muscle and brain. Iron participates in the transport, exchange and tissue respiration of oxygen in the body; maintains normal hematopoietic function, and the iron content in red blood cells accounts for about 2 / 3 of the total iron in the body. In addition, iron is also related to maintaining normal immune function. Studies have found that iron deficiency will reduce lymphocytes and reduce the activity of natural killer cells. In addition, studies have shown that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 马晓明刘楚怡李八方车帅王长伟冯晓梅
Owner OCEAN UNIV OF CHINA
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