Preparation method of small fish protein peptide chelated zinc

A small peptide chelation and fish protein technology, which is applied in the field of fish protein small peptide chelated zinc preparation, can solve the problems of long chelation time, low chelation rate, and difficult control of the chelation process, and achieve short chelation time , High chelation rate, effective control of the effect of the chelation process

Inactive Publication Date: 2017-03-22
青岛海大生物集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of the existing process for preparing chelated zinc, the chelating process is difficult to control, the chelating time is long, and the chelating rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) After dissolving small fish protein peptides, adjust the pH to 5.0-5.5 to obtain a small peptide solution. The mass percentage of the small peptide solution is 20%.

[0027] (2) Add zinc chloride to the small peptide solution, the mass ratio of zinc chloride to small peptide solution is 1:2, fully dissolve, heat up to 35°C, ultrasonically react for 15min, ultrasonic time is 5 seconds, and the interval The time is 2 seconds, and the ultrasonic power is 70%.

[0028] (3) Concentrate the reaction solution to 40% of the original volume after filtration, and obtain fish protein small peptide chelated zinc after spray drying.

[0029] After testing, the chelation rate of fish protein small peptide chelated zinc in this example was 98.2%, and the zinc content was 15.7%.

[0030] According to the preparation method in Yang Huicheng's "A Preparation Process for Fish Skin Collagen Polypeptide Chelated Zinc", the chelation rate of fish skin collagen polypeptide and zinc is 85...

Embodiment 2

[0031] Example 2 Study on the Effect of Small Peptide Chelated Zinc Supplement

[0032] As a zinc deficiency model in mice, the dosage of zinc sulfate group, zinc gluconate, and small peptide chelated zinc was 7.00 mgZn / kg.bw per day, and it was administered orally for 14 consecutive days. Compared with the zinc deficiency group, the small peptide zinc group had a significant difference. Under the same zinc supplementation dose, the serum zinc content of the mice was small peptide zinc group > zinc gluconate group > zinc sulfate group.

[0033] Table 1 Study on the effect of small peptide chelated zinc supplementation

[0034] group Zinc deficiency group small peptide zinc group Zinc gluconate group Zinc sulfate group Zinc content (µg / g) 28.92±1.37 37.09±0.52** 34.30±1.22 32.51±1.53

[0035] Note: Compared with zinc deficiency group, *p<0.05, **p<0.01.

Embodiment 3

[0036] Example 3 Research on Antioxidant Effect of Small Peptide Chelated Zinc

[0037] As a zinc deficiency model in mice, the dosage of zinc sulfate group, zinc gluconate, and small peptide chelated zinc was 7.00 mgZn / kg.bw per day, and it was administered orally for 14 consecutive days. Determination of total antioxidant capacity in mouse liver tissue. Compared with the zinc deficiency group, the small peptide zinc group had a significant difference. Under the same zinc supplementation dose, the order of the total antioxidant capacity of the mouse liver was small peptide zinc group > zinc gluconate group > zinc sulfate group. It shows that under the condition of the same zinc dosage, small peptide zinc is better than zinc gluconate and zinc sulfate.

[0038] Table 2 Research on the antioxidant effect of small peptide chelated zinc

[0039] total antioxidant capacity Zinc deficiency group small peptide zinc group Zinc gluconate group Zinc sulfate group ...

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Abstract

The invention discloses a preparation method of small fish protein peptide chelated zinc. The preparation method comprises the following steps: dissolution of small fish protein peptide, ultrasonic chelation, concentration and spray drying. With the adoption of the process, the problems that the chelating process is not easy to control, the chelating time is long and the chelating rate is low during preparation of chelated zinc with an existing process are solved. The process of a chelating reaction is optimized, and the chelating rate is increased through ultrasonic treatment; the preparation method adopts a simple preparation process step, can effectively control the chelating process and is short in chelating time and easy to operate.

Description

technical field [0001] The invention belongs to the fields of biotechnology and feed processing, and in particular relates to a preparation method of fish protein small peptide chelated zinc. [0002] technical background [0003] Zinc is one of the essential trace elements for animals, with the highest content in tissues and organs such as bones, liver, and fur. Zinc is involved in the composition of more than 300 enzymes and functional proteins in the body. The types of zinc include inorganic zinc, organic zinc, chelated zinc, the first generation of zinc supplements, zinc sulfate and other inorganic reagents, which have a stimulating effect on the gastrointestinal tract and have low biological potency. The second-generation zinc supplements, zinc gluconate, zinc lactate, etc., have better performance than the first-generation zinc supplements, while the third-generation zinc supplements are amino acid chelated zinc and polypeptide chelated zinc. Studies have shown that al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/107
CPCC07K1/107
Inventor 单俊伟王鹏郝晓晨王海华
Owner 青岛海大生物集团股份有限公司
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