73 results about "Fish Proteins" patented technology

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

The invention provides a fish protein amino acid leaf fertilizer preparation control system based on steam explosion. The core of the fish protein amino acid leaf fertilizer preparation control system is that a fish and shrimp mixture is pretreated through a material pretreatment module; performing steam explosion treatment on the fish and shrimp mixture through a steam explosion treatment module; gas phase permeation change characteristics are extracted through the steam explosion control module; carrying out feature learning on the gas-phase permeation change features and the gas-phase permeation influence factors, and determining shell content parameters of the fish and shrimp mixture; carrying out steam explosion process control according to the chitosan target amount and the shell content parameter, and outputting a steam explosion material; the leaf fertilizer preparation module is used for performing leaf fertilizer preparation treatment on the steam explosion material to obtain the fish protein amino acid leaf fertilizer, and the preparation efficiency and the product quality of the fish protein amino acid leaf fertilizer can be improved by extracting the temperature characteristics in the steam explosion process, determining the shell content parameter of the material and performing dynamic steam explosion process control.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer rich in deep sea enzymolysis fish protein and a preparation method of the biological organic fertilizer. The biological organic fertilizer is prepared from the following components in parts by weight: 35 to 45 parts of organic fertilizer (containing deep sea enzymolysis fish protein, tobacco powder, meat powder and bone meal), 6 to 15 parts of compound microorganism (such as bacillus licheniformis and the like), 5 to 10 parts of medium element fertilizer, 1 to 2 parts of trace element fertilizer, 0.1 to 1 part of xanthan gum and sulfur suspension, 30 to 35 parts of outer layer fertilizer (containing humic acid, urea and the like) and 0.1 to 0.5 part of anti-blocking agent. The preparation method comprises the following steps: separately fermenting microorganisms to prepare bacterial powder; mixing, crushing and granulating the raw materials to form master batches; spraying xanthan gum and sulfur suspension on the surface of the master batch to form a film; spraying the outer-layer fertilizer slurry for several times; and finally, an anti-blocking agent is sprayed, and air drying is conducted. The deep sea enzymolysis fish protein is taken as a core, the balance of quick-acting fertilizer efficiency and slow release is realized, the soil structure can be improved, the crop growth is promoted, the stress resistance and the fertilizer utilization rate are improved, the soil-borne diseases are inhibited, and the method has the advantages of high efficiency, environment friendliness, economy, practicability and the like.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention belongs to the field of bioactive protein peptide materials, and provides an animal-derived composite fermented skin-tendering protein peptide and a preparation method thereof. According to the invention, bovine-derived collagen and fish-derived protein are subjected to synergistic fermentation to prepare a protein peptide core, and the outer surface of the protein peptide core is coated with an edible gelatin and chondroitin sulfate composite layer; the excellent performance that a small molecule peptide core with the molecular weight being 500-1500 Da, a stable coating layer with the coating rate being 80-99%, the glycine-proline-hydroxyproline sequence collagen peptide fragment accounting for 30-60% and the pH responsive swelling ratio being 1.5-3.0 are achieved, the technical problems that traditional protein peptide is poor in stability and low in bioavailability are solved, and the protein peptide has wide application value in the field of skin care products.

The invention provides aroma-enhanced soy sauce sauce and a preparation method thereof, and belongs to the field of seasoning processing. The flavor-enhanced soy sauce sauce provided by the invention is prepared from the following raw materials: 500 to 700 kg of soybean fermented soy sauce, 10 to 50 kg of fish protein sauce, 5 to 20 kg of soybean peptide, 40 to 60 kg of sugar, 1 to 4 kg of table salt, 40 to 70 kg of flavoring materials, 0.9 to 1.5 kg of spices and 230 to 270 kg of water. The flavor-enhanced soy sauce sauce provided by the invention is prepared by blending the fish protein sauce prepared from seafood, the soybean peptide prepared from soybeans and the soy sauce, so that the salty and delicious taste of the fish protein sauce and the peptide delicious taste of the soybean peptide are fully fused, and the soy sauce sauce has bright color and rich fragrance, is rich and delicious in taste, mellow, fresh, salty and sweet, and is rich in flavor. And the body is clear.

The invention discloses low-digestibility animal protein-wheat flour composite noodles and a preparation method thereof, and belongs to the technical field of food processing. Wheat flour and exogenous animal protein are used as main raw materials, whey protein isolate, silkworm pupa protein, egg white protein and fish protein are selected, and the addition amount is 15% of the mass of the wheat flour. Through multi-index comprehensive screening, the silkworm pupa protein and whey protein isolate have better market development potential. By utilizing the synergistic effect of the exogenous animal protein and the wheat starch, the content of fast digestible starch (RDS) of the noodles can be remarkably reduced, the ratio of slow digestible starch (SDS) to resistant starch (RS) is increased, and the GI value of the noodles is lower than 70; meanwhile, cooking and texture characteristics are optimized, the cooking loss rate is smaller than or equal to 8%, and the breaking rate is smaller than or equal to 0.3%. The noodles have the characteristics of low glycemic content and high-quality eating taste, are balanced in nutrition, are suitable for diabetics to eat, can also be used as daily staple food of healthy people, and are simple and convenient in preparation process, easy to industrially produce and wide in market application prospect.

The invention discloses a salt-tolerant stable fish protein adhesive emulsion and a preparation method thereof, and belongs to the technical field of food processing. Comprising the following steps: (1) mixing a fish protein glue aqueous solution with a compound polysaccharide aqueous solution to obtain a mixed mother solution; (2) adding sodium chloride into the mixed mother solution, stirring and dissolving to obtain a salt-containing mixed solution; adding edible oil, stirring and mixing to obtain a crude emulsion; and (3) carrying out high-speed dispersion treatment on the crude emulsion to obtain the salt-tolerant stable fish protein adhesive emulsion. According to the invention, the synergistic interaction of sodium alginate and xanthan gum is utilized, the sodium alginate and the xanthan gum form a compact ternary composite network structure with the fish protein glue, the raw materials are food-grade and are safe and non-toxic, the preparation process is simple and controllable, the parameters are clear, special equipment is not needed, and large-scale industrial production is easy; the device is suitable for popularization and application in the field of processing of various foods such as emulsified foods, meat products and seasonings.

The invention belongs to the technical field of feeds, and particularly relates to a biological concentrated feed helpful for removing chicken, duck and pig fishy smell and improving meat quality and fragrance, the biological concentrated feed is prepared from the following components: small molecule peptides, a perilla leaf extract, a clove extract, sodium selenite, zinc sulfate and a carrier; wherein the small molecule peptide is generated by hydrolyzing fish protein. According to the invention, small molecule peptides from fish protein, the perilla leaf extract, the clove extract, the selenium element, new elements and the like are scientifically proportioned innovatively, and all the components act together, so that the fishy smell removal rate can be increased, the meat tenderness can be improved, and the content of meat flavor substances can be increased.

The invention relates to the technical field of food processing, in particular to an alkali-induced glycosylated tilapia protein hydrolysate loaded with fat-soluble polyphenol as well as a preparation method and application of the alkali-induced glycosylated tilapia protein hydrolysate. The glycosylated tilapia mossambica protein hydrolysate is prepared by adopting flavourzyme with mild action conditions for moderate hydrolysis and combining with a green, efficient and reaction degree-adjustable alkali-induced propylene glycol alginate (PGA) glycosylation strategy, so that the water solubility and emulsibility of tilapia mossambica protein are effectively improved, and the tilapia mossambica protein hydrolysate can be used for preparing the tilapia mossambica protein hydrolysate. The glycosylated protein hydrolysate (modified tilapia protein) is used as a carrier to develop an oral delivery system of fat-soluble polyphenols such as quercetin and the like, so that the bioaccessibility of the fat-soluble polyphenols is improved, and the fat-soluble polyphenols can be developed into functional food ingredients to improve the health condition of special groups; the high-value utilization of the tilapia protein in the protein-based polyphenol functional food ingredients is promoted.

The invention relates to a Chryseobacterium-derived protein glutaminase PG3, a coding gene and an application thereof, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG3 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG3 is as shown in SEQ ID No. 2. A large amount of protein glutaminase PG3 from Chryseobacterium is prepared through an Escherichia coli expression system, and the protein glutaminase is used for carrying out enzymatic deamidation modification on the spotted silver carp myofibrillar protein PG3, so that the solubility of the spotted silver carp myofibrillar protein under a salt-free system condition is remarkably improved. The invention provides an important tool enzyme for protein deamidation modification, and lays a theoretical foundation for development of low-salt fish protein processed products.

The invention relates to the technical field of fertilizers, and discloses a biological protein principle-based fertilizer for preventing and treating citrus liberobacter asiaticum, which is prepared from the following raw materials in parts by weight: 40 to 70 parts of fish protein peptide fermentation products, 5 to 15 parts of EM flora active liquid, 1 to 5 parts of chelated trace element solution and 10 to 20 parts of algal polysaccharide-polyglutamic acid compound. The fish protein peptide is fermented for 180 days or longer through EM bacteria. The EM flora active liquid contains lactic acid bacteria, photosynthetic bacteria, actinomycetes and saccharomycetes. The trace elements are at least two of boron, zinc, iron, manganese and molybdenum and are chelated by polyglutamic acid or sugar alcohol. The small peptide, the bacterial metabolite and the chelate element are in slow release synergy, so that rhizosphere microecology can be rebuilt, defensive enzymes can be induced, and the phenomena of huanglong, yellowing and red nose can be relieved.

The invention relates to the technical field of composite culture media, in particular to a composite culture medium and a preparation method and application thereof.The composite culture medium comprises a basic culture medium, chitosan and fish protein, and the addition amount of the chitosan accounts for 0.1-2% of the total weight of the basic culture medium; the addition amount of the fish protein is 1-5% of the total weight of the basic culture medium; the basic culture medium is prepared from the following raw materials in parts by weight: 400 to 600 parts of hard wood chips, 100 to 200 parts of wheat bran, 20 to 50 parts of corn flour, 5 to 15 parts of gypsum powder and 5 to 15 parts of sugar; the fish protein is water-soluble fish protein. According to the composite culture medium, chitosan is combined with fish protein from a specific source, so that the composite culture medium has the excellent performance of promoting rapid growth of hyphae and improving the yield and quality of lucid ganoderma; good infectious microbe pollution resistance and production stability are achieved; the method can be used for modernized factory cultivation of lucid ganoderma, effectively shortens the production cycle, improves the yield of lucid ganoderma, and improves the overall economic value of products.

The utility model discloses a production device of a seaweed fish protein small molecule peptide composition, which belongs to the technical field of production devices, aims to solve the problem of low efficiency when fish bones are cleaned at present, and comprises a base, an electric control box is mounted on the side surface of the base, and a cleaning pipe is mounted on the surface of the base. A material guiding frame capable of guiding out materials in the cleaning pipe is installed on the cleaning pipe, a discharging end for receiving the materials guided out of the material guiding frame is arranged in the cleaning pipe, cleaning operation is conducted in the cleaning pipe during cleaning, then the cleaned materials are guided out through the material guiding frame, and the materials are conveyed to the cleaning pipe through the discharging end. Materials can fall into the discharging end under the action of gravity, so that full-automatic material cleaning operation can be conducted, the fish bone cleaning requirement is met, manual intervention on cleaning is basically not needed, and the situation that a human body is scratched by the fish bones can be effectively avoided.

The invention belongs to the technical field of crop cultivation, and particularly relates to a chemical fertilizer for treating areca yellows as well as a preparation method and application thereof. The chemical fertilizer is prepared from the following raw materials in parts by weight: 10 to 15 parts of microelements, 10 to 15 parts of medium elements, 20 to 25 parts of macroelements, 5 to 10 parts of fish protein, 5 to 10 parts of pyrimidine nucleoside, 5 to 10 parts of humic acid, 5 to 10 parts of brown sugar, 0.7 to 5 parts of compound vitamins, 0.7 to 5 parts of gibberellin, 0.7 to 5 parts of brassinolide, 0.7 to 5 parts of amino-oligosaccharin and 9 to 15 parts of enzyme microorganism. 1-5 parts of salicylic acid and 1-5 parts of carbendazim. When the chemical fertilizer is applied to areca nuts suffering from areca yellows, 1) the acre yield of the areca nuts is increased, 2) the height of the areca nuts is increased, 3) the yellow leaf rate is reduced, 4) the fruit falling rate is reduced, and 5) the stem withering rate is reduced, which prove that the chemical fertilizer prepared by the invention has a remarkable effect on treating the areca yellows.

The invention provides application of protein glutaminase to preparation of salt-reduced, fresh-increased and fishy-smell-removed fish protein gel products, and belongs to the field of food processing. A large amount of protein glutaminase PG3 derived from Chryseobacterium is prepared through a heterologous expression technology, the protein glutaminase PG3 is used for carrying out enzymatic deamidation modification on spotted silver carp MP, and finally, a spotted silver carp MP solution obtained after deamidation is heated and refrigerated to prepare the salt-free thermally-induced MP gel. In addition, the contents of substances with fishy smell, such as hexanal, nonanal and 1-octylene-3-alcohol, in the salt-free heat-induced MP gel are remarkably reduced, and the delicate flavor perception is improved. The method not only plays an important role in fishy smell removal and freshness enhancement of the fish protein gel, but also provides scientific basis and technical support for healthy and sustainable development of high-quality salt-reduced MP gel products.

The present application belongs to the technical field of polypeptide processing, and particularly relates to a preparation method of protein peptide capable of improving flavor and function; the method uses silver carp as raw material, uses food-grade protease, and obtains full fish protein peptide through moderate enzymolysis; under mild conditions, nitrogen is used to replace air to perform glycosylation reaction, which can effectively improve the flavor and antioxidant function of the full fish protein peptide; compared with the original full fish protein peptide and the full fish protein glycopeptide prepared by using air for glycosylation reaction, the full fish protein glycopeptide prepared by the method has lighter fishy smell and bitterness, can effectively improve the acceptance of the public, has strong antioxidant function and good storage stability, and can be widely applied in special food and nutritional food.

The application discloses a method for removing the fishy smell of sea fish protein by using low-temperature plasma and purple flower shannai polyphenol combined treatment. The low-temperature plasma is efficient and easy to operate, and is used for structural modification of sea fish sarcoplasmic protein and myofibrillar protein, so that the structure is unfolded to a certain extent and becomes loose, and the fishy smell substances are more easily removed. In addition, the purple flower shannai polyphenol has stronger combination ability with the hydrophobic group of the protein, the purple flower shannai polyphenol competes with the fishy smell substances for the combination sites, so that the fishy smell substances are released, and the two are synergistic, which is helpful to more thoroughly remove the fishy smell of the sea fish protein. The purple flower shannai polyphenol not only has the deodorizing effect, but also has the antioxidant property and the bacteriostatic property, and the effect of efficiently deodorizing and to a certain extent inhibiting the growth of microorganisms is realized.

The invention provides high-foamability fish protein glue and a preparation method thereof. The method comprises the following steps: S1, preparing a fish protein glue solution; s2, dissolving Arabic gum in the fish protein glue solution obtained in the step S1; s3, adjusting the pH value of the solution obtained in the step S2, and adding an enzyme for enzymolysis; s4, performing enzyme deactivation on the materials subjected to enzymolysis, and adjusting the pH value again after enzyme deactivation; and S5, freezing the material obtained in the step S4 at low temperature, and freeze-drying to obtain the high-foamability fish protein glue. The preparation process is simple, no pollution is generated in the treatment process, and the fish protein glue has the advantages of being green and environmentally friendly, the foamability of the prepared fish protein glue reaches up to 158.89%, and the foamability stability reaches up to 55.40%.

The utility model discloses a ceramic reaction kettle for animal protein extraction, which relates to the technical field of animal protein and comprises a ceramic reaction kettle body, a sealing cover is arranged at the top end of the ceramic reaction kettle body, a protective shell is arranged in the middle of the sealing cover, a mixing component penetrating through the top end of the sealing cover is arranged in the protective shell, and a feeding pipe is arranged on one side of the top of the sealing cover. A feeding pipe is arranged on the other side of the top of the sealing cover, a control panel is arranged on the front face of the ceramic reaction kettle body, a filtering assembly matched with the mixing assembly is arranged at the inner bottom of the ceramic reaction kettle body, a discharging pipe matched with the filtering assembly is arranged at the bottom end of the ceramic reaction kettle body, and an electromagnetic valve is arranged outside the circumference of the discharging pipe. The ceramic reaction kettle is reasonable and reliable in structure and simple to operate, fish protein liquid can be extracted through the ceramic reaction kettle body, and a mixture in the ceramic reaction kettle body can be stirred and mixed, so that the mixture in the ceramic reaction kettle body is more uniformly mixed.

The invention relates to the technical field of agricultural fertilizers, in particular to a rooting and root protecting type organic water-soluble fertilizer. The fertilizer is prepared from the following raw materials in parts by weight: 15-23 parts of deep-sea fish protein enzymatic hydrolysate, 8-16 parts of humic acid, 6-12 parts of a seaweed extract, 6-10 parts of potassium fulvate, 3-10 parts of amino acid, 2-5 parts of a compound microbial agent, 2-5 parts of chitosan oligosaccharide, 1-2 parts of polyglutamic acid, 2-8 parts of monopotassium phosphate, 1-4 parts of medium trace elements, 1-2 parts of a plant-derived growth induction factor extract and the balance of water. The components in the rooting and root-protecting type organic water-soluble fertilizer disclosed by the invention are synergistic, the functions are comprehensive, and the rooting and root-protecting type organic water-soluble fertilizer has the advantages of rapid rooting, strong root protection, efficient stress resistance and comprehensive nutrition. The invention further provides a preparation process of the rooting and root protecting type organic water-soluble fertilizer, and a two-stage fermentation-low-temperature chelation process is adopted. According to the process, all organic components are fully degraded and biologically converted, the biological value is improved, the probiotics and the organic carrier are perfectly fused, and the high survival rate and colonization capacity after the probiotics are applied to soil are ensured.

This application belongs to the field of fish protein gelatin preparation technology, specifically relating to a method for preparing high-purity fish protein gelatin and its deodorization process. Pretreated fish scales are soaked in a citric acid solution to remove calcium. The soaked fish scales are then added to water and sheared to obtain a fish protein gelatin extract. Solid-liquid separation is performed, followed by drying to obtain high-purity fish protein gelatin. This extract is then dissolved in water with xylose and mixed thoroughly, followed by freeze-drying. The resulting freeze-dried sample undergoes superheated steam-assisted glycosylation. After glycosylation, the glycosylated fish protein gelatin is dissolved to obtain deodorized fish protein gelatin. This invention achieves efficient extraction and deep deodorization of fish protein gelatin through an integrated process of low-temperature high-speed shearing and superheated steam-assisted glycosylation, significantly improving product purity and providing a feasible technical foundation for the industrial application of fish protein gelatin in high-end fields such as food, pharmaceuticals, and cosmetics.

The invention relates to the technical field of agricultural fertilizers, in particular to a fertilizer, a preparation method thereof and application of the fertilizer to improvement of tomato fruit quality. The fertilizer is prepared from 5 percent to 15 percent (v / v) of fish protein hydrolysate, 0.05 to 0.2 mmol / L of 1-aminocyclopropane-1-carboxylic acid and water. The preparation method of the fertilizer comprises the following steps: mixing the fish protein hydrolysate (the amino acid content is greater than or equal to 100g / L) with water in proportion, adding the ACC, stirring until the ACC is completely dissolved, sequentially adding the trace element compound and the seaweed extract, adding the environment-friendly surfactant, adjusting the pH value to 6.0-6.5, and uniformly mixing to obtain the fertilizer. The fertilizer provided by the invention can be used for improving the tomato fruit quality, and after the fertilizer is applied, the tomato yield can be increased by 15-25%, the fruit sugar degree can be increased by 25.87%, the vitamin C content can be increased by 20-30%, and the shelf life can be prolonged by 2-3 days. The fertilizer is completely prepared from biodegradable components, is free of chemical synthesis hormone residues and is environment-friendly.

The invention provides a crop nutritional supplement containing glycopeptide as well as a preparation method and application of the crop nutritional supplement, and belongs to the technical field of biological agriculture. The crop nutritional supplement comprises fish protein peptide, fucose oligosaccharide, fulvic acid, silicon-calcium-potassium-magnesium fertilizer, bone meal, polyglutamic acid, a microbial agent and a carrier. Wherein the fish protein peptide is obtained by fermenting deep-sea fish meat with bacillus subtilis SH21 and performing enzymolysis with compound enzyme, and the microbial agent is prepared from trichoderma atroviride C61 and paenibacillus mucilaginosus HB-02. The crop nutritional supplement can activate the immune system of crops, enhance disease resistance, promote nutrient absorption and improve the yield and quality of crops, is environment-friendly, and can solve the problems caused by chemical pesticides and traditional chemical fertilizers.

1. The name of the design product: bottle stickers (seaweed polypeptide fish protein). 2. The use of the design product: bottle stickers for packaging bottles. 3. The design points of the design product: the combination of shape and pattern. 4. The picture or photo that best indicates the design points: perspective view. 5. The design product is a flat product, omitting the left view, right view, top view, and bottom view.

The present application belongs to the technical field of biological medicine, and particularly relates to a pharmaceutical composition for treating Parkinson syndrome. Researches show that the Antarctic fish protein and the multi-domain derivative thereof have the functions of reducing PD pathology and protecting dopaminergic neurons.

The invention discloses a method for improving the comprehensive performance of a fish protein glue composite film and the fish protein glue composite film, and relates to the technical field of food film package preparation. The preparation method comprises the following steps: S1, mixing a fish protein glue solution, a gellan gum solution and a microbial glutamine transaminase solution to obtain a first mixed solution; s2, adding glycerol into the first mixed solution, and stirring to obtain a second mixed solution; s3, the second mixed solution is placed in a mold, crosslinking and drying are conducted at the preset temperature and the preset humidity at the same time, demolding is conducted, and the fish protein glue composite film is obtained. When the fish protein glue prepared by the method provided by the invention is applied to the manufacturing process of the film, the safety performance of the film can be effectively improved, the advantage of degradability is beneficial to environmental protection, and in addition, the film produced by adopting the fish skin protein glue has the advantages of degradability, edibility, good comprehensive performance and the like.

The invention discloses a preparation method of low-salt minced spanish mackerel gel based on egg white protein, and belongs to the field of food processing. The preparation method comprises the following steps: S1, blending spanish mackerel to obtain minced spanish mackerel meat; s2, adding salt into the minced spanish mackerel meat, and blending to obtain low-salt minced spanish mackerel meat; s3, egg white protein is added into the low-salt minced spanish mackerel for blending, and low-salt egg white protein-minced spanish mackerel is obtained; and S4, performing two-stage heating on the low-salt egg white protein-minced spanish mackerel fillet to obtain the low-salt minced spanish mackerel fillet gel. According to the preparation method disclosed by the invention, the egg white protein is used for replacing spanish mackerel protein, so that the texture property of the low-salt minced spanish mackerel gel is improved, the indexes such as gel strength, water binding capacity and cooking loss of the low-salt minced spanish mackerel gel are ideal, and the network structure is more uniform and compact.

The invention discloses a mackerel ACE inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. According to the invention, five brand-new ACE inhibitory peptides are successfully identified from mackerel protein for the first time, and the amino acid sequences of the five brand-new ACE inhibitory peptides are shown as SEQ ID NO.1-5. Five brand-new ACE (angiotensin converting enzyme) inhibitory peptides are successfully identified from mackerel protein for the first time, and cell experiments prove that the peptides not only show clear antihypertensive potential, but also show excellent anti-oxidative stress capability, and have antihypertensive and anti-oxidative dual activities. The natural source of the ACE inhibitory peptide is expanded, a core raw material is provided for developing a novel antihypertensive functional product, and the additional value and the resource utilization efficiency of the mackerel are greatly improved.

The invention relates to chitophage protein glutaminase PG5, a coding gene and application of the chitophage protein glutaminase PG5, and belongs to the technical field of biological genetic engineering. The amino acid sequence of the protein glutaminase PG5 is as shown in SEQ ID No. 1, and the cDNA sequence of the coding gene of the protein glutaminase PG5 is as shown in SEQ ID No. 2. A large amount of chitophage-derived protein glutaminase PG5 is prepared through an escherichia coli expression system, and the protein glutaminase PG5 is used for carrying out enzymatic deamidation modification on the myofibrillar protein of the spotted silver carp, so that the thermal reversible protein gel is successfully prepared in a salt-free system. An important tool enzyme is provided for protein deamidation modification, unprecedented flexibility is provided for processing adaptability and cold-chain storage and transportation of fish protein gel products, and the application range of the fish protein gel products in food design is greatly expanded.