350 results about "Fish Proteins" patented technology

The invention relates to a preparation method of a soluble fish protein ammonia acid fertilizer, belongs to the field of biological technical processing, and particularly belongs to the field of fertilizer additive preparation. The preparation method comprises the following steps: crushing and pulping leftovers from fishery product processing or deep-sea low-value shrimp into fish pulp, mixing with water, adding lactic acid bacillus and protease, and fermenting for 3-8 days respectively through continuous ventilation and interval ventilation at a temperature of 40-45 DEG C to obtain the soluble fish protein ammonia acid fertilizer. The preparation method disclosed by the invention is simple, and short in heating treatment time, so that the heating cost is saved and the loss of nutrient substances is reduced. The fish protein ammonia acid soluble fertilizer prepared by adopting the preparation method has a good application effect, and particularly has remarkable effect on fruits and vegetables.

The invention belongs to the technical field of organic fertilizer production. In order to fully utilize nutrient substances in offal fish and leftovers generated after fish processing, the invention provides a method for producing a water-soluble fish protein organic fertilizer from fish, which comprises the following steps: raw material treatment, fermentation and enzyme hydrolysis, physical separation, water-soluble enzymolysis solution purification treatment, concentration, drying and packaging. The production method has the advantages of severe steps and scientific parameter setting, can prevent environmental pollution generated by fish leftovers, changes the waste leftovers into valuable substances, and can implement centralized collection and treatment on the leftovers generated by aquatic product processing enterprises.

The present invention relates to an insoluble composition comprising an acylated protein selected from the group consisting of acylated insulin, acylated insulin analogs and acylated proinsulin and preparations thereof. The formulations are suitable for parenteral or other delivery to a patient for prolonged control of blood glucose levels. More specifically, the present invention relates to compositions comprising an acylated protein complexed with zinc, protamine and a phenolic compound such that the resulting microcrystals resemble the neutral protamine zinc (NPH) insulin crystalline form. Surprisingly; this acylated protein composition has been found to have therapeutically superior subcutaneous release pharmacokinetics, and longer and flatter glucose kinetics than currently marketed NPH insulin formulations. Furthermore, the crystals of the present invention retain some of the advantageous properties of NPH crystals, namely being able to be easily resuspended and also mixed with soluble insulin.

The present invention relates to an anchovy enzymatic protein seasoning liquid and a preparation method thereof. Fresh or frozen and unfrozen anchovy is used as a raw material. Papain, compound protease and flavor enzyme are combined as compound enzyme to be hydrolyzed as short peptide protein liquid. After anchovy protein enzymatic hydrolysate is processed for killing enzyme, residue and liquid are separated to obtain hydrolysate solution; active carbon with the weight of 1 percent to 3 percent of the weight of the hydrolysate solution is added for filtering; 0.5 percent to 1 percent of beta-cyclic dextrin is added for debitterizing; the solution is condensed to 1/2 to 1/3 of the original volume after centrifugal filtration; colorant with the weight of 0.05 percent to 0.2 percent of the weight of the condensed anchovy protein enzymatic hydrolysate, 0.05 percent to 0.2 percent of emulsifier and 5 percent to 25 percent of seasoning are added for homogenizing and seasoning. The seasoning liquid preserves all nutrient contents and functional factors of anchovy, has high nutrient value and fresh and dense taste, is easy for digesting and absorbing and has the health care efficacies on reducing cholesterol, eliminating fatigue, improving body immunity, etc. The preparation method has reasonable technology, simple operation and strong operability and is easy for realizing the industrialized production.

The invention provides a nibea albiflora richardson larvae and juvenile microcapsule feed which is prepared from white fish meal, euphausiid meal, squid meal, fish protein hydrolysate, feed yeast, alpha-starch, soybean lecithin, papain, fish oil, composite vitamin, composite mineral substance, binder, immunopotentiator, phagostimulant and the like. By adopting the innovated prescription and processing process, the microcapsule feed can fulfill the requirement for nutrition of larvae and juvenile growth, has the advantages of comprehensive nutrition, good palatability, high water stability, low cost and the like, can be produced in batches, is convenient for storing, transporting and feeding, is suitable for industrial breeding production, and adopts a corresponding feeding method, so that the survival rate of nibea albiflora richardson is greatly improved, the shape of the nibea albiflora richardson is increase. The nibea albiflora richardson larvae and juvenile microcapsule feed is suitable for large-scale popularization and application.

The invention discloses a nutritious energy rod and its preparing process, wherein the rod is prepared from malt sugar 5-20%, levulose 5-20%, glucose 10-42%, oat powder 3-15%, soybean polypeptide 3-16%, fish protein small peptide 2-12%, fish bone meal 1-5%, cocoa powder 2-10%, onion 3-15%, sea-tangle 2-8%, vitamin 0. 3-3%, microelements 0. 1-2%, amino acid 0. 5-5%, taurine 0. 1-2%, choline 0. 05-1%, soybean lecithin 2-8%, DHA fish oil 3-12% and edible gelatin 1-8%.

The invention discloses a detection method for the distribution of fish protein content based on a hyper-spectral imaging technology. The detection method comprises the following steps: (1) acquiring single-waveband spectrum images of a fish to be detected at eleven characteristic wavelengths, wherein the eleven characteristic wavelengths are 951nm, 999nm, 1038nm, 1110nm, 1144nm, 1167nm, 1210nm, 1229nm, 1383nm, 1555nm and 1632nm respectively; (2) converting the single-waveband spectrum images in the step (1) into reflection rate images according to a linear relation between gray values and reflection rates of the single-waveband spectrum images; and (3) calculating according to a formula to obtain corresponding fish protein content of each pixel point in the reflection rate images of the fish. The detection method for the distribution of the fish protein content is high in detection precision and short in detection time; the environmental pollution is reduced and the detection cost is further reduced to certain extent.

The invention discloses an antioxidant peptide of hairtail fish protein as well as a preparation method and uses of the antioxidant peptide. In particular, hairtail fish is used as raw materials; an enzymatic hydrolysis solution is obtained through degreasing and enzymolysis of protamex and neutral protease; the enzymatic hydrolysis solution is separated and purified through ultra-filtration, purification of macroporous resins, cation exchange resin chromatography, gel column chromatography, and reversed-phase high-performance liquid chromatography so as to obtain the antioxidant peptide Asn-Trp-Asp-Met-Glu-Lys-Ile-Trp, and the ESI-MS determining molecular weight is 1121.25da. The high-activity antioxidant peptide disclosed by the invention has a good scavenging effect on DPPH free radicals, hydroxyl radicals and superoxide anion free radicals; besides, the antioxidant peptide shows a good effect of lipid peroxidation inhibition. The antioxidant peptide has the advantages of safe use, zero toxic or side effects, high oxidative activity and the like, is liable to digest and absorb, and can be used as medicines, health food or food additives, and the like.

The invention discloses a production method of fish protein hydrolyzed chelate. The production method includes: preprocessing residual waste of low-value marine fishes such as ribbonfish, adding complex enzymes to hydrolyze the waste, and subjecting polypeptides to chelate modification. The fish protein hydrolyzed chelate has effects of antibiosis, antioxidation and ferric strengthening. The fish protein hydrolyzed chelate is purely natural, has good antioxidation, and has evident antibacterial effects on escherichia coli, bacillus subtilis, staphylococcus aureus and salmonella. Absolute ethyl alcohol can be recycled in production. Production cost is low. High-value development and high utilization of aquatic products are achieved.

The invention relates to a method for preparing bonito stick protein hydrolysate with an effect of reducing uric acid. The method comprises the following main steps of raw material heat treatment, restriction digestion, membrane separation-anion exchange chromatography-gel filtration chromatography separation, concentration and spray drying so as to obtain the bonito stick protein hydrolysate with an effect of reducing uric acid. The amino acid analysis indicates that the zymolyte peptide fragment primary amino acid sequence contains four amino acids, namely histidine, arginine, lysine and threonine, the total mass content of the four amino acids is 70 percent of the total amino acid content of zymolyte. MALDI-TOF-MS mass spectrum determines that the molecular weight of the main peptide effective ingredient is less than 700Da. In-vitro uric acid reduction experiments prove that the bonito stick protein hydrolysate has a remarkable effect of inhibiting generation of uric acid, and has an inhibition rate over 50 percent; an oteracil potassium molded hyperuricemic rat animal model indicates that the bonito stick protein hydrolysate can be used for remarkably reducing the level of serum uric acid and serum creatinine of rats, and shows a relatively good kidney protecting effect.

The invention discloses an ecological environmental protection fish protein liquid fertilizer. The ecological environmental protection fish protein liquid fertilizer comprises the following components in parts by weight: 1 to 50 parts of fish and shrimp protein hydrolysate, 1 to 50 parts of seaweed hydrolysate, 1 to 20 parts of molasses, 1 to 20 parts of urea, 1 to 20 parts of ammonium sulfate, 1 to 20 parts of potassium dihydrogen phosphate, 1 to 5 parts of zinc sulfate, 1 to 5 parts of boric acid, 1 to 5 parts of magnesium sulfate, 1 to 5 parts of ferrous sulfate, 0.5 to 2 part of manganese sulfate, 0.5 to 2 parts of copper sulfate and 0.1 to 1 part of sodium molybdate. According to the invention, waste fish sauce, shrimp heads, shrimp shells and seaweeds in processing of aquatic products are biologically treated, and converted into bioactive peptides, amino acids, seaweed polysaccharides and alginate nutrients which are easily absorbed by plants, and inorganic salts are added for manufacturing the ecological environmental protection fish protein liquid fertilizer for agricultural planting.

The invention discloses a composite all-fish protein powder and a preparation method thereof and belongs to the field of aquatic product processing. The method comprises: mincing fish to make minced fillet, mixing different kinds of minced fillets and adding water into the mixture to make minced fillet pulp; performing enzyme hydrolysis and centrifugation, concentrating obtained supernate and performing the spray drying of the concentrate to obtain fish protein polypeptide powder; washing sediment, drying the sediment in hot air, crushing the sediment and sieving the crushed sediment by a 100-mesh sieve to obtain fish protein powder; and mixing the fish protein polypeptide powder and the fish protein powder to obtain the composite all-fish protein powder. The fish protein polypeptides in the composite all-fish protein powder can be absorbed and utilized by human bodies at a high rate; the composite all-fish protein powder has higher nutritive value and better mouthfeel than fish protein powder processed by using single freshwater fish; the composite all-fish protein powder is 100 percent fish protein powder obtained by hydrolysis of food grade-composite proteinase; the method of the invention is simple and low in cost; and the composite all-fish protein powder can be conveniently eaten as direct nutrition food or a food additive.

The invention discloses a fish protein organic compound fertilizer and a preparation method thereof. The fish protein organic compound fertilizer is prepared by using a deep marine fish as a main raw material and adopting the steps of fermenting through microorganisms to form a liquid base fertilizer and adding copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, molybdenum salt, fulvic potassium, EDTA (Ethylene Diamine Tetraacetic Acid) and the like. The fish protein organic compound fertilizer is rich in fish protein and multiple trace elements, is favorable for improving the soil, promoting the aggregation, improving the water retention and ventilation of the soil and eliminating the hardening, and is suitable for various vegetables, fruit trees and field crops.

The invention discloses a complex microbial agent used for ocean trash fish fermentation, and a preparation method for an enzymatic fish protein amino acid water-soluble fertilizer. The complex microbial agent consists of any three or four types of bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus cereus, lactic streptococci and saccharomyces cerevisiae. The preparation method for the enzymatic fish protein amino acid water-soluble fertilizer comprises the following steps of: before ocean trash fishes are transported, the ocean trash fishes are subjected to pretreatmentthrough the complex microbial agent, breaking, enzymolysis, fermentation through the complex microbial agent, separation, filtering and concentration. The enzymatic fish protein amino acid water-soluble fertilizer is rich in various active substances and trace elements, including micromolecule polypeptides, amino acids, vitamins and the like, can meet the nutrition requirements of fruit and vegetable crops, improves the growth performance of fruit and vegetable crops as well as the quality of fruits and vegetables, reduces the use amount of chemical fertilizers, improves the quality of soil, reduces soil problems, including soil secondary salinization and the like, and has a good economic benefit.

The invention relates to a preparation method of iron chelating peptide derived from hairtail fish protein. The preparation method concretely comprises the following steps: taking hairtail fish as a raw material, carrying out enzymolysis for the hairtail fish protein with papain and trypsin, carrying out ultrafiltration, macroporous resin column chromatography, gel column chromatography and reversed-phase high performance liquid chromatography separation and purification, and thus obtaining the iron chelating peptide Ala-Pro-Pro-Glu-Asn-Gly-Met-Ala-Gln-Met, wherein the ESI/MS determining molecular weight is 1045.18Da. Due to the unique chelating and transport mechanism, the iron chelating peptide of hairtail fish protein is high in absorption availability, can simultaneously supplement polypeptide/amino acid and iron, and is an ideal iron supplement substance.

A fish protein-biologic peptides fertilizer is prepared from the leftover of fresh fish, fish skin or fish bone through pulverizing, regulating pH=1-6.5, adding trypsase or pepsin, degradating, filtering concentrating and sterilizing. It is rich in biologic peptide, amino acids, Ca, P, Zn, Cu, Se, Fe, trace element, vitamines, etc.

The invention discloses a method for preparing peptone by utilizing fish processing wastes, belonging to the technical field of biology. The method is characterized in that the raw materials used for preparing peptone are fish processing wastes; the raw materials are minced and degreased to obtain minced fillet pulp; a certain amount of protease is added; protease enzymolysis procedures are controlled by applying size-exclusion high performance liquid chromatography (SEC-HPLC); membrane separation is further used for desalination and concentration, and the powder obtained after spray drying is fish peptone. The method has the beneficial effects that the fish processing wastes can be fully utilized by adopting the method to prepare high-quality fish peptone; the method has the advantages of retention of full amino acid composition, high content of amino nitrogen, low protein molecular weight, low ash content, and the like; the product can serve as health food, a nutrient enhancer, a food additive, a feed additive, a medium in the fermentation industry, and the like.

The invention discloses a low carbon type litopenaeus vannamei ecological fodder and a preparation method thereof. The fodder comprises the following components: bean pulp, fish meal, flour weevil powder, chicken powder, inkfish powder, earthworm powder, fly maggot powder, silkworm chrysalis meal, shrimp head shell powder, rapeseed dregs, rice bran, wheat flour, corn flour, enteromorpha algae powder, saccharomyces cerevisiae, fish oil, soybean oil, phospholipid oil, folium mori flour, monocalcium phosphate, choline chloride, compound ore, compound vitamins, vitamin C phosphate and phytase. The fodder has scientific compatibility of medicines; the fish protein is replaced by the insect protein mainly from flour weevil, so that the dependence on the imported fish meal can be reduced, the cost is lowered and the low carbon purpose is achieved; the bioactive substances including folium mori flour, alga, and the like, are added, so that the factor interference of anti-nutrients can be reduced; the prawn can fully utilize the fodder; the growth performance of the prawn can be promoted; the emission amount of nitrogen and phosphorus can be reduced, so that the breeding environment of the prawn can be improved.

A formulated, microbound diet product for the culture of larval fish and crustaceans either in a dry or moist form is disclosed. The food product contains protein sources such as fish protein hydrosylate, casein, egg yolk, binding agents such as soy lecithin, wheat gluten, and alginate. Other ingredients such as vitamins and minerals, lipid sources, carbohydrate sources, pigment sources, and attractant compounds are included in the diet for nutritional completeness. A method for preparation of the food product is also disclosed.

The invention provides an efficient maca cultivation method. The method comprises the following steps of raising of maca seedlings in a greenhouse, soil preparation and moisture, transplanting, field management and harvesting. The method is characterized in that film mulching is adopted during the transplanting of the maca seedlings, and 500 times liquid of fish protein is sprayed for 2-3 times at a time interval of 6-8 days in the most vigorous period of maca leaves. By adopting the film mulching during the transplanting of the maca seedlings, weeds are decreased, a great deal of intertillage weeding work is avoided, and the production cost is reduced; besides, the nutrient demand in an expansion period of rootstocks is fully satisfied and the output and the quality of the maca are improved because of the topdressing in the most vigorous growth period of the maca leaves; and moreover, the revenue of the maca per Mu is enhanced while the cost of the maca per Mu is reduced, and the effects of excellent quality, high output and high efficiency of maca production are achieved.

The invention discloses a sea fish protein peptide and a preparation method thereof. The method comprises the following steps: rinsing fish with alkaline saline water and water in turn, adding water and heating to 85-95 DEG C, heating for 10-25 minutes, quickly cooling to 2-5 DEG C, standing and discarding the upper liquid and grease to obtain protein; regulating the protein concentration in the system to 30-50% w/v, heating to 45-60 DEG C, sequentially adding 100-150U/g of trypsin and 250-450U/g of bacillus subtilis protease according to the protein weight, controlling the concentration of solid content to 10-15%, deactivating enzyme and centrifuging the hydrolysate to obtain enzymatic hydrolysate; and filtering with a ceramic membrane, an organic ultrafiltration membrane and a nanofiltration membrane. The method breaks through the traditional preparation mode and technology of fish collagen, the equipment is simple and easy to operate, and the method is suitable for large-scale production; and the prepared protein peptide has good activity and high and stable quality, and has good functions of immunoregulation, fatigue resistance and oxidation resistance.

The process of preparing fish protein fertilizer includes the steps of: crushing fresh fish skin, regulating pH value with sulfuric acid to 5, stirring, adding certain amount of trypsin, biodegrading at 37 deg.c for 30 hr, filtering, and concentrating at 37 deg.c to obtain the product. The present invention has the advantages including capacity of providing plant with biological protein possessing high permeability and nourishing function, capacity of raising the activity of isoenzyme and other enzymes, and the synergistic effects of raising plant resistance, improving crop quality and promoting plant growth.

The invention discloses ready-to-eat fish meat protein power, and belongs to the field of aquatic product processing. The ready-to-eat fish meat protein power is obtained by cooking fish meat of seawater fish at high temperature and high pressure, pouring a fat-containing solution on the upper layer, then adding water, performing enzymolysis on the fish meat respectively by using neutral proteinase and flavourzyme, performing centrifugating after the enzymolysis is finished, desalting supernate obtained by centrifugating to obtain desalted supernate, adding water into precipitates obtained by centrifugating, performing enzymolysis on the precipitates by using papain to obtain fish protein zymolyte, finally mixing the desalted supernate and the fish protein zymolyte, and homogenizing, concentrating, spraying and drying the mixture to obtain the ready-to-eat fish meat protein power. The invention also discloses a method for preparing the ready-to-eat fish meat protein power. The ready-to-eat fish meat protein power is high in protein content and high in polypeptide content and is high in dissolvability and convenient to eat; and furthermore, the preparation method is low in energy consumption and low in production cost.

The invention provides a method for producing a low-value fish sausage by the combined fermentation of mixed strains and belongs to the technical field of food processing. The method comprises the following steps of: removing bones from low-value seawater or freshwater fish which are taken as a raw material, homogenating meat, adding a compound flavoring agent according to certain formula, stirring uniformly, pickling at the temperature of between 0 and 4 DEG C for 8 to 24 hours, inoculating the mixed grains, filling into a casing, fermenting at the temperature of between 30 and 37 DEG C for 18 to 24 hours, sterilizing at high temperature, cooling and packaging. The method is characterized in that: the mixed strains of lactic acid bacteria, staphylococcus and saccharomycetes are used for the combined fermentation, so that the fermentation efficiency can be effectively improved, the gel performance of fish protein is strengthened, and the fish sausage with excellent nutrition, quality and flavor is obtained. The method is a safe, efficient and novel method for producing fermented aquatic products; and by the method, low-value seawater or freshwater fish resources in China can be fully utilized, and a novel way is provided for the processing, appreciation and transformation of low-value fish.

The invention discloses a method for producing fish protein and fish protein peptide and belongs to the technical field of biology. The method comprises the following steps: (1) oil removing equipment isolation technology in coordination with an oil-water separating unit isolation technology is adopted to remove solid impurities and fats and oils in minced fillet waste water; (2) a membrane separation technology is adopted to separate, purify and concentrate fish protein solution; (3) fish protein powder finished product is obtained after the fish protein solution is dried; (4) an enzymic method is adopted to dispose fish protein solution obtained through step (2), so as to prepare fish protein peptide; (5) fish protein peptide powder finished product is obtained after the fish protein peptide solution is dried. According to the method, the minced fillet waste water is adopted to change waste into valuable; an isolation technology system which is integrated by coordination with the oil removing equipment isolation technology, the oil-water separating unit isolation technology with the membrane separation technology is established; the problems that membrane pollution is serious, the service life is short in a conventional minced fillet waster water research and development technology are solved; the production cost is reduced; the producing fish protein products has high protein content; the safety of fish protein peptide product is high; the relative molecular weight is low averagely.

The invention relates to a preparation method of a low-value extraction recombinant minced hairtail product. The preparation method comprises the steps: mixing minced hairtail and a soybean protein isolate with a weight ratio of 1:4 in a chopper mixer, and extruding by adopting a homodromy twin-screw extruder, wherein the water content of a raw material is 30-50 percent, the rotating speed of screws is 80-120rpm, the temperature of the screws is 140-160 DEG C, and the optimal process condition is optimized by using a response surface method. Compared with that of a raw material, the in-vitro digestibility coefficient of the protein is increased by 10.25 percent. According to the preparation method, the low-value minced hairtail is recombined by adopting the homodromy twin-screw extruder, the advantages of high temperature, high pressure and high shearing force of an extrusion system are fully utilized, the degradation of macromolecules in a cylinder is promoted, the ordering transition of a protein array in the material is accelerated, the texturization and the specific retention of the material are improved; meanwhile, through passivating enzyme and damaging antinutritional factors, the in-vitro digestibility coefficient of the protein in the material is increased, and the nutritive value of the material is improved.