Sea fish protein peptide and preparation method thereof

A marine fish and protein peptide technology, which is applied in the field of marine fish protein peptide and its preparation, can solve the problems that the quality of marine fish protein peptide products cannot meet market requirements, the low proportion of deep processing of marine fish protein resources, and the removal of bitterness and color. Achieve good immune regulation, rich variety, and good product activity

Active Publication Date: 2015-04-08
GUANGDONG SHINYEE MARINE BIOLOGY ENG CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, the proportion of deep processing of marine fish protein resources is low, and resources are seriously wasted
In the fish protein deep processing industry, fish collagen peptide is a relatively mature product at present, but the part of its development and utilization is the extracellular protein—collagen in the muscle matrix protein, and the intracellular peptide with high active ingredien

Method used

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  • Sea fish protein peptide and preparation method thereof
  • Sea fish protein peptide and preparation method thereof
  • Sea fish protein peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of marine fish protein peptide described in this embodiment comprises the following steps:

[0035] (1) Use 150kg of sardines as raw materials, remove the fish head and viscera (because the fish head, viscera and black film contain relatively high oil and fishy bitter substances, so remove), wash the blood stains, black film and other things under running water, drain and weigh weight, calculated rate;

[0036] (2) the above-mentioned pretreated raw fish is harvested by a meat harvester, and the fish skin and fish bones are reclaimed as collagen peptide raw materials. The purpose of harvesting meat is mainly to utilize myofibrillar protein and sarcoplasmic protein of fish meat;

[0037](3) Rinse the above-mentioned fish with alkaline brine in a ratio of 1:3 by weight in an ultrasonic cleaning tank for 15min, frequency 32K / HZ, and then use clear water in a ratio of 1:5 by weight of meat to water Further rinsing in the ultrasonic cleaning tank for ...

Embodiment 2

[0055] The preparation method of marine fish protein peptide described in this embodiment comprises the following steps:

[0056] (1) Use 100kg of blue squid as raw material, remove fish head and viscera (because fish head, viscera and black film contain high oil and fishy bitter substances, so remove), wash blood, black film and other things under running water, drain Dry weighing, calculated yield;

[0057] (2) the above-mentioned pretreated raw fish is harvested by a meat harvester, and the fish skin and fish bones are reclaimed as collagen peptide raw materials. The purpose of harvesting meat is mainly to utilize myofibrillar protein and sarcoplasmic protein of fish meat;

[0058] (3) Rinse the above-mentioned fish meat with alkaline brine in an ultrasonic cleaning tank at a weight ratio of 1:4 for 10 minutes at a frequency of 36K / HZ, and then use clear water to clean it in an ultrasonic cleaning tank at a weight ratio of 1:6.5 between fish meat and water. Further rinsing...

Embodiment 3

[0073] The preparation method of marine fish protein peptide described in this embodiment comprises the following steps:

[0074] (1) Take 100kg of anchovies as raw material, wash and cut open the abdomen or back, remove the fish head and viscera (because the fish head, viscera and black film contain high oil and fishy bitter substances, so remove), wash with running water Blood stains, black film, etc., drain and weigh, and calculate the yield;

[0075] (2) the above-mentioned pretreated raw fish is harvested by a meat harvester, and the fish skin and fish bones are reclaimed as collagen peptide raw materials. The purpose of harvesting meat is mainly to utilize myofibrillar protein and sarcoplasmic protein of fish meat;

[0076] (3) Rinse the above-mentioned fish with alkaline brine in a ratio of 1:5 by weight in an ultrasonic cleaning tank for 20min, frequency 38K / HZ, and then use clear water in a ratio of 1:8 by weight of meat and water. Further rinsing in the ultrasonic c...

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Abstract

The invention discloses a sea fish protein peptide and a preparation method thereof. The method comprises the following steps: rinsing fish with alkaline saline water and water in turn, adding water and heating to 85-95 DEG C, heating for 10-25 minutes, quickly cooling to 2-5 DEG C, standing and discarding the upper liquid and grease to obtain protein; regulating the protein concentration in the system to 30-50% w/v, heating to 45-60 DEG C, sequentially adding 100-150U/g of trypsin and 250-450U/g of bacillus subtilis protease according to the protein weight, controlling the concentration of solid content to 10-15%, deactivating enzyme and centrifuging the hydrolysate to obtain enzymatic hydrolysate; and filtering with a ceramic membrane, an organic ultrafiltration membrane and a nanofiltration membrane. The method breaks through the traditional preparation mode and technology of fish collagen, the equipment is simple and easy to operate, and the method is suitable for large-scale production; and the prepared protein peptide has good activity and high and stable quality, and has good functions of immunoregulation, fatigue resistance and oxidation resistance.

Description

technical field [0001] The invention relates to the field of marine biological products and biotechnology processing, in particular to a marine fish protein peptide and a preparation method thereof. Background technique [0002] Protein peptide (IGF-1), also known as human growth factor, is an active protein polypeptide substance, a molecular polymer between amino acids and proteins, mainly composed of 2 to 10 amino acids, it is the liver cells in the human body, The autocrine and paracrine products of more than a dozen kinds of cells such as kidney cells and spleen cells have very important biological significance. , promote growth, promote cell differentiation, wound repair, etc. In recent years, as the value of protein peptides has been paid more and more attention, extensive research has been carried out on the functional activities and preparation processes of protein peptides from different raw materials at home and abroad. The relatively mature protein peptide produc...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/46C07K1/34
Inventor 任增超王炎冰潘建文
Owner GUANGDONG SHINYEE MARINE BIOLOGY ENG CORP
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