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Complex microbial agent used for ocean trash fish fermentation, and preparation method for enzymatic fish protein amino acid water-soluble fertilizer

A technology of compound microbial inoculants and compound enzyme preparations, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as soil compaction, improve soil ecology, optimize soil structure, and achieve dark green leaf color. Effect

Active Publication Date: 2020-01-31
安徽瑞驰兰德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the combined use of compound microbial agents and compound enzyme preparations, fish protein amino acid water-soluble fertilizers with high amino acid content are prepared to solve the problems of soil compaction, soil secondary salinization, and reduce the use of chemical fertilizers, improve crop quality, and increase crop yield. Increase economic income and realize high-value, resourceful and ecological utilization of low-value marine biological proteins

Method used

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  • Complex microbial agent used for ocean trash fish fermentation, and preparation method for enzymatic fish protein amino acid water-soluble fertilizer
  • Complex microbial agent used for ocean trash fish fermentation, and preparation method for enzymatic fish protein amino acid water-soluble fertilizer
  • Complex microbial agent used for ocean trash fish fermentation, and preparation method for enzymatic fish protein amino acid water-soluble fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing an enzymatically hydrolyzed fish protein amino acid water-soluble fertilizer, comprising the following steps:

[0035] (1) Stir the trash fish and the compound microbial agent evenly before transportation, perform pretreatment, and then pack them in plastic bags for normal temperature transportation;

[0036] (2) The pretreated marine trash fish is broken into 0.2cm fragments with a bone mud machine;

[0037] (3) Add water to the broken sea fish in a mass ratio of 1:2, and raise the temperature to 60°C, add a compound enzyme preparation for enzymolysis for 12 hours; then repeat this step to the enzyme obtained from the first enzymolysis Add the same amount of compound enzyme preparation to the hydrolyzate again to carry out the second enzymolysis to obtain the enzymolyzate;

[0038] (4) Send the enzymatic hydrolysis solution to the fermentation kettle and add the compound microbial bacterial agent, ferment at 25°C for 24 hours, fully stir during th...

Embodiment 2

[0044] A method for preparing an enzymatically hydrolyzed fish protein amino acid water-soluble fertilizer, comprising the following steps:

[0045] (1) Stir the trash fish and the compound microbial agent evenly before transportation, perform pretreatment, and then pack them in plastic bags for normal temperature transportation;

[0046] (2) The pretreated marine trash fish is broken into 0.2cm fragments with a bone mud machine;

[0047] (3) Add water to the crushed marine trash fish according to the mass ratio of 1:3.3, and raise the temperature to 65°C, add a compound enzyme preparation for enzymolysis for 8 hours; Add the same amount of compound enzyme preparation to the hydrolyzate again to carry out the second enzymolysis to obtain the enzymolyzate;

[0048] (4) Send the enzymolysis liquid to the fermentation tank and add the compound microbial bacterial agent, ferment at 40°C for 20 hours, fully stir during the fermentation process, and obtain fermented fish soluble pu...

Embodiment 3

[0054] A method for preparing an enzymatically hydrolyzed fish protein amino acid water-soluble fertilizer, comprising the following steps:

[0055] (1) Stir the trash fish and the compound microbial agent evenly before transportation, perform pretreatment, and then pack them in plastic bags for normal temperature transportation;

[0056] (2) The pretreated marine trash fish is broken into 0.2cm fragments with a bone mud machine;

[0057] (3) Add water to the broken sea fish in a mass ratio of 1:2.7, and heat up to 63°C, add a compound enzyme preparation for enzymolysis for 10 hours; Add the same amount of compound enzyme preparation to the hydrolyzate again to carry out the second enzymolysis to obtain the enzymolyzate;

[0058] (4) Send the enzymatic hydrolysis solution to the fermentation kettle and add the compound microbial bacterial agent, ferment at 25°C for 24 hours, fully stir during the fermentation process, and obtain fermented fish soluble pulp after the fermentat...

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Abstract

The invention discloses a complex microbial agent used for ocean trash fish fermentation, and a preparation method for an enzymatic fish protein amino acid water-soluble fertilizer. The complex microbial agent consists of any three or four types of bacillus licheniformis, bacillus subtilis, bacillus megaterium, bacillus cereus, lactic streptococci and saccharomyces cerevisiae. The preparation method for the enzymatic fish protein amino acid water-soluble fertilizer comprises the following steps of: before ocean trash fishes are transported, the ocean trash fishes are subjected to pretreatmentthrough the complex microbial agent, breaking, enzymolysis, fermentation through the complex microbial agent, separation, filtering and concentration. The enzymatic fish protein amino acid water-soluble fertilizer is rich in various active substances and trace elements, including micromolecule polypeptides, amino acids, vitamins and the like, can meet the nutrition requirements of fruit and vegetable crops, improves the growth performance of fruit and vegetable crops as well as the quality of fruits and vegetables, reduces the use amount of chemical fertilizers, improves the quality of soil, reduces soil problems, including soil secondary salinization and the like, and has a good economic benefit.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a compound microbial bacterial agent for fermenting marine trash fish and a preparation method of enzymatically hydrolyzed fish protein amino acid water-soluble fertilizer. Background technique [0002] Fish protein fertilizer is mainly a kind of fertilizer that uses deep-sea fish and shrimp, combined with enzymatic hydrolysis and microbial low-temperature bacterial hydrolysis fermentation technology, and uses deep-sea fish protein as the main raw material. After enzymolysis, it is rich in various active substances such as small molecule polypeptides, amino acids, taurine, vitamins, etc., as well as medium and trace elements such as calcium and magnesium. Types of. [0003] However, the fish protein fertilizer prepared in the prior art requires strict temperature control during the enzymatic hydrolysis and fermentation process, which requires relatively...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C05C11/00C12R1/10C12R1/125C12R1/11C12R1/085C12R1/46C12R1/865
CPCC05C11/00C12N1/18C12N1/20
Inventor 刘晓龙徐钰青芮文东王道胜王二燕
Owner 安徽瑞驰兰德生物科技有限公司
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