591 results about "Protein amino acid" patented technology

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate alkynyl amino acids such as para-propargyloxyphenylalanine into proteins produced in a eubacteria host such as E. coli. The invention provides novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing alkynyl amino acids, and cellular translation systems.

The present invention relates to a composition for the delivery of active agents that are protected from environmental oxidation and are resistant to ruminal fermentation degradation but not intestinal digestion and adsorption. The composition may contain biologically active ingredients such as for example, vitamins, minerals, proteins, amino acids, fatty acids, nutritional supplements, and pharmaceuticals together with natural and/or synthetic lecithin phospholipids.

The present invention relates to a series of prodrugs of inhibitors of DP-IV with improved properties. The compounds can be used for the treatment of a number of human diseases, including impaired glucose tolerance and type II diabetes. The compounds of the invention are described by general formula (1); wherein R¹ is H or CN; R² is selected from CH₂R⁵, CH₂CH₂R⁵ and C(R³)(R⁴)—X²—(CH₂)_aR⁵; R³ and R⁴ are each independently selected from H and Me; R⁵ is selected from CON(R⁶)(R⁷), N(R⁸)C(=0)R⁹, N(R⁸)C(═S)R⁹, N(R⁸)SO₂R¹⁰ and N(R⁸)R¹⁰; R⁶ and R⁷ are each independently R¹¹(CH₂)_b or together they are —(CH₂)₂-Z-(CH₂)₂— or CH₂-o-C₆H₄-Z-CH₂—; R⁸ is H or Me; R⁹ is selected from R¹¹(CH₂)_b, R¹¹(CH₂)_bO and N(R⁶)(R⁷); R¹⁰ is R¹¹(CH₂)_b; R¹¹ is selected from H, alkyl, optionally substituted aryl, optionally substituted aroyl, optionally substituted arylsulphonyl and optionally substituted heteroaryl; R¹² is selected from H₂NCH(R¹³)CO, H₂NCH(R¹⁴)CONHCH(R¹⁵)CO, C(R¹⁶)═C(R¹⁷)COR¹⁸ and R¹⁹OCO; R¹³, R¹⁴ and R¹⁵ are selected from the side chains of the proteinaceous amino acids; R¹⁶ is selected from H, lower alkyl (C₁-C₆) and phenyl; R¹⁷ is selected from H and lower alkyl (C₁-C₆); R¹⁸ is selected from H, lower alkyl (C₁-C₆), OH, O-(lower alkyl (C₁-C₆)) and phenyl; R₁₉ is selected from lower alkyl (C₁-C₆), optionally substituted phenyl and R²⁰C(=0)OC(R²¹)(R²²); R²⁰, R²¹ and R²² are each independently selected from H and lower alkyl (C₁-C₆); Z is selected from a covalent bond, —(CH₂)_c—, —O—, —SO_d— and —N(R¹⁰)—; X¹ is S or CH₂; X² is O, S or CH₂; a is 1, 2 or 3; b is 0-3; c is 1 or 2; and d is 0, 1 or 2.

The present invention relates to compositions and methods for the treatment and diagnosis of conditions, disorders, or diseases involving cell death. The invention encompasses protective nucleic acids which, when introduced into a cell predisposed to undergo cell death or in the process of undergoing cell death, prevent, delay, or rescue the cell from death relative to a corresponding cell into which no exogenous nucleic acids have been introduced. The invention encompasses nucleic acids of the protective sequence, host cell expression systems of the protective sequence, and hosts that have been transformed by these expression systems, including transgenic animals. The invention also encompasses novel protective sequence products, including proteins, polypeptides and peptides containing amino acid sequences of the proteins, fusion proteins of proteins, polypeptides and peptides, and antibodies directed against such gene products. The invention further relates to target sequences, including upstream and downstream regulatory sequences or complete gene sequences, antibodies, antisense molecules or sequences, ribozyme molecules, and other inhibitors or modulators directed against such protective sequences, protective sequence products, genes, gene products, and/or their regulatory elements involved in cell death. The present invention also relates to methods and compositions for the diagnosis and treatment of conditions, disorders, or diseases, involving cell death, including, but not limited to, treatment of the types of conditions, disorders, or diseases, which can be prevented, delayed or rescued from cell death and include, but are not limited to, those associated with the central nervous system, including neurological and psychiatric conditions, disorders, or diseases, and those of the peripheral nervous system. Further, the invention relates to methods of using the protective sequence, protective sequence products, and/or their regulatory elements for the identification of compounds that modulate the expression of the protective sequence and/or the activity of the protective sequence product. Such compounds can be useful as therapeutic agents in the treatment of various conditions, disorders, or diseases involving cell death.

A method and apparatus for separating the organic membrane portion of waste egg shells from the hard inorganic mineral portion thereof, so that each can be used or further processed as desired, thereby addressing the environmental and economic issues associated with disposing of waste egg shells. The membrane and shell are separated by first processing waste eggshells so as to yield small waste eggshell particles. During this processing, the waste eggshell particles are at least slightly abraded, whereby the linking structure between the membrane and shell is at least partially disrupted. Thereafter, the waste eggshell particles are conveyed to a device, which isolates the two components based on their size and weight differences. The methods listed above can provide products from the eggshell waste including precipitated calcium carbonate, membrane, protein, amino acids, collagen and other important components.

The invention discloses an anti-aging skincare mask containing crithmum maritimum. The anti-aging skincare mask is prepared from a skin conditioner component, an auxiliary component and water, wherein the skin conditioner component is prepared from 2%-6% by mass of butanediol, 2%-5% by mass of glycerin, 0.1%-0.5% by mass of a crithmum maritimum extract, 1%-5% by mass of a pseudoalteromonas fermentation product extract, 1%-5% by mass of acetyl hexapeptide-8, 0.5%-3% by mass of a Centella asiatica extract, 0.5%-2% by mass of D-panthenol, 0.03%-0.1% by mass of sodium hyaluronate, 1%-5% by mass of Chamomile German water, 0.5%-3% by mass of corn protein amino acids, 1%-3% by mass of a lotus extract, 0.5%-2% by mass of bio-saccharide gum-1 and 0.1%-0.3% by mass of allantoin. The anti-aging skincare mask containing crithmum maritimum can inhibit lipid peroxidation and inflammatory reaction, rebuild water balance and brighten complexion and has effects of preserving moisture, resisting aging, brightening the complexion and realizing anti-allergic repair.

The invention discloses gene engineering application of rice auxin transport protein gene OsPIN2, which belongs to the field of gene engineering. The accession numbers of the nucleotide sequence of the rice auxin transport protein gene OsPIN2 and the expression OsPIN2 protein amino acid sequence thereof are AK101191 in an NCBI website (www. ncbi. nlm. nih. Gov/). The engineering application of the gene is firstly reported in the rice, and participates in the transport of rice auxin so as to increase the rice roots, tiller number, tiller angle, nitrogen utilization efficiency and finial output.

The invention discloses a method for predicting flu antigen through a model and an application thereof. The method comprises the following steps: extracting 12 features affecting the flu antigen, wherein the 12 features include mutation numbers of five antigenic determinant amino acid, five physical and chemical properties of HA protein amino acid, factor affecting receptor, and number of glycosylated site, the five physical and chemical properties of amino acid include hydrophobicity, volume change, electriferous property, polarity and integrable surface area; and making statistics on the 12 features of 3681 virus pairs similar to known antigen and 1720 virus pairs variant from antigen, thereby establishing a prediction model on antigen relation. On the basis of sequence, the method can present the antigen relation among viruses, and thus having the advantages of simpleness, convenience and high sensitivity. The process of antigen evolution can be visually displayed through the network. The invention is used for revealing flu spreading rules, screening vaccine candidate strains and the like.

A protein powder features its ideal protein-amino acid structure mode and contains soybean protein, whey protein, stabilizer, lecithin and 9 amino acids necessary to human body. Its preparing process features use of boiling bed to spray lecithin and granulating.

The invention relates to a method for preparing germinated oats. The method comprises the steps of selecting raw materials, performing wet-type careful selection, soaking, accelerating the germination of oats, drying germinated oats and packaging the dried germinated oats. Scientific tests show that the nutrient components of the germinated oats prepared by the method have the following changes as compared with oats: 1, a change in protein and amino acid: antihypertensive functional nonprotein amino acid gamma-aminobutyric acid increases by 10 to 40 times; 2, a change in carbohydrate: the rough mouthfeel of oats is obviously improved, so that people is easy to accept the oats; 3, a change in mineral elements: the content of the mineral elements is increased, and the absorption rate of the mineral elements in human bodies is raised; 4. a change in vitamins: the vitamin content of the oats after germination obviously increases; 5, a change in functional factors: as the content of the functional factors of the oats greatly increases, and as a plurality of active enzymes of the oats are fully activated and released, the oats are good in the function of resisting oxidation and favorable for eliminating free radicals in human bodies; and 6, a change in dietary fiber: the function of the oats in treating various chronic diseases is strengthened. The method has the advantages of scientific process flow, extensive raw materials, eating convenience and easy popularization.

The invention relates to environment-friendly type textile sizing agent. The environment-friendly type textile sizing agent comprises following components in parts by mass: 72 parts of nano silicon dioxide modified starch, 15 parts of a protein sizing agent, 11 parts of polyacrylate emulsion, 2 parts of an organic silicon de-foaming agent, 3 parts of protein amino acid, 4 parts of kaolin, 3 parts of sodium sulfamate and 1 part of polyacrylate. The sizing agent with the formula has good film-forming property and permeability; the formula does not contain polyvinyl alcohol, thus the environment-friendly type textile sizing agent is very environment-friendly.

The invention discloses a high performance liquid chromatography separating column suitable for amino acid chiral resolution. The separating column is prepared by the following steps: dissolving R(or S)-(3,3'-bromo-1,1'-dinaphthyl)-20-crown-6 in dichloromethane, uniformly dispersing the crown ether solution to C18 silica gel, carrying out rotary evaporation to remove the solvent to prepare a chiral stationary phase; and mixing the chiral stationary phase in a methanol water solution, stirring to obtain a homogenate solution, and filling the chromatographic column by a wet process by using the methanol water solution as a displacement fluid. The separating column can effectively separate all the 19 protein amino acids with chiral site at normal temperature, and can effectively separate chiral drugs with primary amine on the chiral site. The separating column has the obvious characteristics of high resolving power, high separating rate, high reproducibility, lower preparation cost and the like, and can be used repeatedly. The separating column has obviously better separating effect for amino acids than the like products at home and abroad.

The invention discloses a low-protein daily ration of a three-way cross growing pig and belongs to the technical field of a pig industry of animal husbandry. The formula of the daily ration comprises 66-68 parts of corns, 4-4..6 parts of wheat bran, 15.4-24 parts of soybean meal, 0.88-0.92 part of stone powder, 0.89-1.07 parts of calcium hydrophosphate, 0.4 part of edible salt, 0.168-0.44 part of L-lysine hydrochloride. 0.040-0.081 part of DL-methionine, 0-0.12 part of L-threonine, 0-0.03 part of L-tryptophan, 0.208-0.582 part of zeolite powder and 1.00 part of premixed powder. According to the technology disclosed by the invention, according to an ideal protein amino acid model of the three-way cross growing pig, the rough protein level of the daily ration of the three-way cross growing pig are reduced by three percent, so that the aims of keeping the growing performance, reducing the cost of a feed formula and reducing nitrogen content in excrements are achieved.

The invention discloses a cordyceps taishanensis ferment product and a production method thereof. A main method comprises the following steps: selecting moringa tree saw dust powder, millets and rice as main materials; adding a suitable amount of glucose and peptones as cordyceps taishanensis culture base materials; mashing a great amount of cordyceps taishanensis mycelia generated in the base materials, and mixing the mashed cordyceps taishanensis mycelia with rock candies, honey, aspergillus and the like; bottling and sealing; brewing to generate a moringa cordyceps taishanensis mycelium edible ferment raw solution; or mixing the edible ferment raw solution and dextrin according to a suitable ratio; and drying in air to prepare a ferment buccal tablet food. The innovation points are that the health-care plant moringa trees and five cereals including the millets and the rice which have abundant nutrition are developed and used as the raw materials; the cordyceps taishanensis mycelia are cultured to prepare the ferment product; the ferment product is abundant in nutrition, convenient to eat and good for body health. The product contains proteins, amino acids, vitamins, microelements and the like needed by a human body; functional active components including cordycepin, cordycepic acid, cordyceps polysaccharides, SOD (Superoxide Dismutase) and the like are effectively accumulated; various health-care effects of the ferment are effectively improved.

The invention discloses a full-length nucleotide sequence and a mature peptide nucleotide sequence of a mink growth hormone releasing hormone cDNA gene, and encoded protein amino acid sequences thereof. The mink growth hormone releasing hormone cDNA sequence is a complete open reading frame with 321 nucleotide sequences, and 106 amino acids are encoded. The mature peptide is encoded by 132 nucleotide sequences, and 44 amino acid residues are encoded. The mink growth hormone releasing hormone cDNA can be used for promoting the growths of economic animals such as minks.

The invention discloses a fast-growing aquatic plant nitrate transport protein GeNRT2.1 and a coding gene and application thereof. The protein is the protein a or the protein b or the protein c, wherein the protein a is provided with an amino acid sequence shown in the sequence 5 in a sequence table; the protein b is a fusion protein obtained by connecting labels to the end N and/or the end C of the protein shown in the sequence 5 in the sequence table; the protein c has the same function and is the protein obtained through substitute and/or deletion and/or addition of one or more amino acid residues for the amino acid sequence shown in the sequence 5 in the sequence table. A test proves that the protein GeNRT2.1 has the function of a nitrate transport protein and can improve the plant nitrogen utilization efficiency, so that the plant grows rapidly.

The invention relates to a method for brewing black millet iron-rich nutritional soy, which mainly overcomes the shortage of low organic iron content in general soy taking soybeans and wheat as raw materials and the shortcoming of addition of an iron additive into finished soy, and can be used for increasing iron-containing microelements. The method comprises the following step: with black crops with high organic iron content as raw materials, carrying out biochemical reaction on various enzyme systems under specific environmental conditions through a combined constant-temperature fermentation process of a low-salt solid-state fermentation process and a high-salt diluted-state fermentation process of various strains to enable iron microelements and other nutritional ingredients such as proteins, amino acids and vitamins in the raw materials to be fully decomposed and effectively enriched in soy so as to generate soy with unique color, aroma, taste and shape and high content of various nutritional ingredients such as organic iron.

The invention discloses a gene of a recombinant staphylococal protein A and application thereof. The nucleotide sequence of the gene of the recombinant staphylococal protein A is shown as SEQ ID NO:1; and the amino acid sequence of the protein coded by the gene is shown as SEQ ID NO:2. The staphylococal protein A is fused with small ubiquitin to form the gene of the recombinant staphylococal protein A; the expressed recombinant protein facilitates purifying the protein and improves the stability and activity of the protein; and experiments prove that the bioactivity of the expressed recombinant staphylococal protein A is not obviously different from that of a natural staphylococal protein A. The recombinant staphylococal protein expression and purification method has the advantages of short production time, high expression efficiency, high expression level, simple purification and the like.

The invention relates to a compound protein amino-acid feed which mainly solves the technical problem that technical personnel in a feed industry applies a grasped digestible amino acid formulation technology to a livestock and poultry simple-stomach animal scale livestock farm which carries out self blending by using pre-mixing materials at present to substitute for a mealie- bean pulp type high-crude protein formulation technology which is used all the time at present and on the basis of total amino acids. The compound protein amino-acid feed comprises the following materials in percentage by weight: 14%-73.5% of conventional protein feeds, 26%-83% of unconventional protein feeds and 0.5%-3% of essential amino acids. According to the invention, compound protein amino acids are applied to the production practice proving of the livestock and poultry simple-stomach animal scale livestock farm which carries out the self blending by using the pre-mixing materials. The compound protein amino-acid feed disclosed by the invention can conserve the proteins and the amino acids, reduce the environmental pollution and sufficiently utilize the unconventional protein feed resources and is an environmental-friendly saving type feed.