30 results about "Ruminal fermentation" patented technology

The invention relates to the field of pasturing livestock nutrition and feed resource development, and particularly discloses an application method of a digestion and emission reduction functional composite preparation for pasturing cattle in saline-alkali soil, and the application method comprises the following steps: S1, selecting Allium mongolicum and Stellera chamaejasme as raw materials, and screening an optimal mixing ratio through in vitro rumen fermentation; s2, preparing a granular composite preparation according to the optimal proportion; s3, supplementary feeding is conducted on the saline-alkali land grazing cattle according to the fixed dosage. The method solves the problems of low digestibility and high methane emission of saline-alkali soil grazing cattle due to ingestion of low-quality forage grass, and solves the technical gaps that Allium mongolicum and Stellera chamaejasme are wasted as traditional toxic weed resources, and single grass seeds are difficult to synergistically realize digestion promotion and methane emission reduction, improves the digestibility of saline-alkali soil grazing cattle and nitrogen utilization efficiency, and improves the economic benefit of the saline-alkali soil grazing cattle. Methane emission is synchronously reduced, toxic weeds are converted into functional indigenous grass, the feed source is expanded, the breeding cost is reduced, the technology adapts to the grazing scene, operation is easy and convenient, and large-scale application and popularization are easy.

The invention discloses application of thiamine in preparation of a product for treating subclinical ketosis of dairy cows, and belongs to the field of biological medicines. Tests prove that compared with dairy cows suffering from subclinical ketosis (SCK), by adding thiamine, the content of beta-hydroxybutyric acid (BHBA), the number of bacteria in milk and the number of somatic cells can be remarkably reduced, and the milk yield, the milk fat content, the acetic acid content and the A / P ratio are remarkably increased; the microbial diversity of rumen and excrement of the dairy cow can be adjusted; a metabonomics result shows that metabolite up-regulated after the thiamine is added mainly focuses on pyruvic acid metabolism, glycolysis or gluconeogenesis, tyrosine metabolism, glycerophospholipid metabolism and biosynthetic pathways of phenylalanine, tyrosine and tryptophan. The conclusion is that when thiamine is added into the SCK dairy cow, synthesis and catabolism of BHBA of an organism can be effectively regulated and controlled by adjusting the content of succinyl-coenzyme A, then subclinical ketosis is relieved, rumen fermentation is promoted, and therefore the milk yield and the milk quality are improved.

The application provides application of a walnut green peel extract in preparation of rumen fermentation regulation products, and belongs to the technical field of feed preparation.The walnut green peel extract in the application is prepared by extracting walnut green peel with an extraction solvent, concentrating the extract into a paste under reduced pressure, and drying and crushing the paste at low temperature.Adding 0.5% of the walnut green peel extract to a high-concentrate sheep feed has the effects of preventing the decrease of rumen pH, increasing the activities of rumen cellulose-degrading enzymes and the acetic acid level, and significantly reducing the concentrations of propionic acid and valeric acid.The experimental results show that, after the rumen fermentation regulation product in the application is used, the average rumen pH of sheep in a whole day is increased by 0.24, the filter paper enzyme activity is increased by 73%, the cellobiase activity is increased by 66%, the acetic acid is increased by 29%, and the propionic acid and valeric acid are respectively reduced by 28% and 43%, which indicates that the product in the application can play a high-efficiency rumen fermentation regulation role in the high-concentrate feeding process of rumen animals.

The present application belongs to the technical field of in-vitro rumen fermentation simulation device, and particularly relates to a quantitative sampling device for an in-vitro rumen continuous fermentation system, comprising a fermentation equipment main body, a sampler fixed to the outer wall of the fermentation equipment main body, the sampler comprising an outer support tube, and a plurality of equidistantly arranged sampling tubes fixed to the outer wall of the outer support tube and communicated with each other. When it is needed to take out the sampling cup which has completed quantitative sampling from the gravity positioning assembly, the operator can manually pinch the two connecting rods in reverse. The action drives the T-shaped handle to be lifted from the notch where it is currently located through linkage of the two transmission rods, and drives the inner rotating tube connected therewith to move upward synchronously. At the same time, the two auxiliary plates move towards each other, drive the two clamping rods to move away from each other under cooperation of the second elastic sheet, thereby releasing the positioning constraint on the sampling cup. With the conical opening at the bottom end of the inner rotating tube lifted, sufficient space is formed below the conical opening, so that the sampling cup can be smoothly taken out.

The invention discloses a fattening feed additive composition for old cattle and a fattening method, and belongs to the technical field of feed. The additive composition comprises the following components in parts by weight: 13-20 parts of a rumen function regulator, 4-10 parts of an intestinal repair agent, 10-20 parts of a rumen bypass compound enzyme preparation, 5-10 parts of a muscle-increasing growth-promoting composition, 5.5-7.3 parts of a functional trace element composition and 32.7-62.5 parts of a carrier. Wherein the rumen function regulator is compounded by a coated saccharomyces cerevisiae culture and bacillus subtilis; the rumen bypass compound enzyme preparation comprises acid protease, neutral protease and cellulase; the muscle-building and growth-promoting combination is coated guanidinoacetic acid. Through regulation and control of a rumen-intestinal tract-tissue three-level linkage network, the rumen fermentation function of the old cattle is remarkably improved, the feed conversion rate is increased, and meanwhile muscle hyperplasia and meat tenderization are achieved. By means of the fattening method, the average daily gain of the old cattle is increased by 22.8% or above, the muscle shear force is reduced by 29.6% or above, the feed conversion ratio is reduced by 17.1% or above, and green and efficient fattening is achieved.

The invention belongs to the technical field of animal feed, and discloses a fermented total mixed ration for fattening mutton sheep based on agricultural by-products and a preparation method of the fermented total mixed ration. The fermented total mixed ration is prepared by taking apple pomace, tomato pomace and traditional Chinese medicine residues as main raw materials, matching with high-quality forage grass such as silage corn, alfalfa and a small amount of concentrated feed, regulating and controlling the raw material ratio, inoculating lactobacillus plantarum or lactobacillus buchneri and fermenting under an anaerobic condition. The preparation method is simple in process and good in fermentation stability, the dosage of concentrated feed in daily ration can be effectively reduced, and the feed cost is remarkably reduced. A fattening test verifies that the feed intake, the average daily gain and the feed conversion ratio of mutton sheep can be improved by feeding the fermented total mixed ration disclosed by the invention, meanwhile, the rumen fermentation performance is improved, the antioxidant and immune functions are enhanced, and the dressing percentage and the meat quality are improved. The method realizes efficient feed utilization of agricultural byproducts, and has good economic benefits, ecological benefits and popularization and application values.

The invention belongs to the technical field of livestock feed, and particularly discloses a total mixed ration containing fermented cotton straw and application thereof. The total mixed ration comprises the following raw materials on the basis of dry matter: 5-15% of fermented cotton straw, 10-20% of corn straw, 5-10% of alfalfa, 25% of corn, 9% of soybean meal, 7.5% of dry corn vinasse, 10% of corn germ meal, 15% of soybean hull, 0.9% of calcium hydrophosphate, 0.4% of ammonium chloride, 0.2% of choline chloride, 0.5% of sodium chloride and 0.5% of sodium bicarbonate. The total mixed ration can significantly improve the growth performance and feed conversion efficiency of the Hu sheep, improve the digestion and utilization rate of nutrient substances, effectively improve the health and anti-stress level of animal bodies, improve the diversity and structure of rumen microflora, promote the growth of beneficial microorganisms, and establish a more stable and efficient rumen fermentation system.

The invention belongs to the technical field of agricultural waste resource utilization, and particularly relates to an application of a black ink fruit extract in preparation of a product for reducing methane emission and / or nitrogen excretion of animals, a preparation method of the black ink fruit extract comprises the following steps: drying black ink fruits, and crushing the dried black ink fruits to obtain black ink fruit powder; taking ethanol with a volume fraction of 60% as an extraction solvent, adding the black ink fruit powder for extraction, and collecting an extract liquid; and centrifuging, decolorizing, filtering, concentrating and freeze-drying the leaching solution to obtain the black ink fruit extract. According to the invention, it is proved for the first time that the black ink fruits can inhibit a methane generation key approach and reduce ammonia nitrogen concentration by regulating rumen fermentation while not damaging feed basic digestibility and animal health. The method not only directly brings emission reduction and environmental protection benefits, but also can improve the utilization efficiency of feed energy and protein, improve the milk protein rate and the feed conversion rate, and realize double improvement of environment and production benefits.

The invention discloses an amaranthus hypochondriacus silage total mixed ration formula and a feeding method, and relates to the technical field of silage. Comprising the following raw materials: modified amaranthus hypochondriacus silage stalks, amaranthus hypochondriacus silage leaves, corn silage, alfalfa hay, corn, modified cottonseed meal, soybean meal, wheat bran, a buffering agent, a functional agent, modified attapulgite, a cattle fattening premix, stone powder and table salt. According to the beef cattle feed disclosed by the invention, the added modified grain amaranth silage stalks can reduce the digestion barrier of beef cattle to coarse feed, guarantee the utilization efficiency of daily ration fibers, and avoid the influence on the feed intake and the weight gain effect of the beef cattle due to too high proportion of low-digestion fibers; the added modified cottonseed meal provides crude protein with high utilization rate for beef cattle, and ensures the protein deposition and growth performance of the beef cattle in the fattening period; the added functional agent can inhibit the reproduction of harmful flora, improve the digestion and utilization rate of the whole feed, and guarantee the stability and high efficiency of rumen fermentation.

The application discloses acetic acid-producing proteobacteria and application thereof in preparation of feed additives. 35 strains with good fiber degradation function are obtained from Holstein cow rumen fluid through preliminary screening, and bran medium is used for screening the 35 strains, and it is found that 25 strains have the ability of converting complex carbon source into reducing sugar, and the most powerful strains of different genera are selected for in-vitro fermentation verification, and the fiber utilization efficiency of the strains on ruminant TMR is observed, and it is found that the acetic acid-producing proteobacteria (microbial preservation number is CGMCC No. 33516) has the most powerful function on feed dry matter degradation rate and fiber degradation rate, and can improve rumen fermentation liquid pH value, and significantly improve the content of acetic acid, isobutyric acid, butyric acid, valeric acid and total rumen volatile fatty acid. The acetic acid-producing proteobacteria provided by the application has application prospect in improving feed fiber utilization rate or improving animal digestion capacity.

The invention belongs to the technical field of animal nutrition and feed, and particularly relates to a feed composition, a method for improving fattening performance and meat quality of down producing goats and application, corn straw is used for replacing 40-60% of high-quality forage grass in basic daily ration of the down producing goats, and artemisia ordosica crude polysaccharide accounting for 0.08-0.12% of the total dry matter weight of the daily ration is added; experimental results show that the feed composition can significantly improve the average daily gain (up to 45.25%) of the down producing goats, reduce the feed conversion ratio (up to 27.32%), and improve the apparent digestibility of nutrient substances, the rumen fermentation function and nitrogen metabolism; meanwhile, the slaughter performance can be improved, the meat quality can be improved, physiological and biochemical indexes of blood can be adjusted, and immunity and metabolic health can be enhanced; efficient utilization of corn straw resources is achieved, the problem of shortage of high-quality roughage is solved, and remarkable economic and ecological benefits are achieved.

The present application belongs to the technical field of feed, and is a fermented feed for ruminants and a preparation method thereof. The fermented feed for ruminants is obtained by fermenting soybean straw and rice straw after being mixed with a fermentation agent. The fermentation agent comprises Lactobacillus plantarum, Pediococcus pentosaceus, Bacillus subtilis and Lactobacillus brevis. It is found that the mixture of the four bacteria has a certain synergistic effect on the fermentation of soybean straw, which can effectively improve the nutritional value of the feed. When the mixture of the four bacteria is used for the fermentation of the mixture of soybean straw and rice straw, the synergistic effect is more obvious, which not only solves the problem of the ensiling utilization of soybean straw, but also improves the nutritional value of the feed, effectively reduces the methane production in rumen fermentation, and can bring the effect of methane emission reduction to the ruminant breeding industry.

The application discloses a kind of bionic rumen fermentation mechanism and self-adapting control method, it is related to microbial fermentation and bionic equipment technical field.The mechanism mainly includes by outer to inner in turn tightly covered bionic drive outer layer, response deformation middle layer and bionic contact inner layer, and air intake and exhaust assembly and control unit.Bionic drive outer layer inlay shape memory alloy drive warp / weft line, for simulating rumen large peristalsis;Response deformation middle layer array distribution has independent response chamber encapsulated intelligent response material, through electric shrinkage and expansion simulating microperistalsis;Bionic contact inner layer densely covers bionic flexible papilla, for the flexible contact and kneading of material.Control unit is combined with gas / liquid two-phase sensor data, identifies fermentation material state, and accordingly self-adapting control large peristalsis and microperistalsis mode of operation.The application can effectively simulate the mechanical action of natural rumen, reduce the shear damage of traditional rigid stirring to microorganism, significantly improve the fermentation efficiency and mixed uniformity of high solid content material.

This invention discloses a methane inhibitor based on plant functional components and its application. The inhibitor, by mass fraction (100%), comprises the following raw materials: 1.5-6% p-hydroxybenzoic acid, 0.4-2.4% astragaloside, 5-40% polyethylene glycol 4000, and 81.6-99.999% bentonite. The methane inhibitor provided by this invention uses p-hydroxybenzoic acid and astragaloside as active ingredients. Through their synergistic effect, it effectively inhibits the activity of methanogenic bacteria and the methane production pathway in the rumen of ruminants, while simultaneously improving the rumen fermentation pattern. The above-mentioned methane inhibitor uses natural plant-derived active ingredients, is safe, non-toxic, environmentally friendly, and has a simple preparation process suitable for large-scale production.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The invention relates to the technical field of feed additives, in particular to a sustained-release feed additive containing eucalyptus globulus essential oil for rumens of cattle and sheep. The CO2-triggered eucalyptus globulus essential oil sustained-release capsule comprises a CO2-triggered eucalyptus globulus essential oil microcapsule unit, a cobalt acetate sustained-release microsphere unit and a carrier unit. Wherein the microcapsule unit is of a three-layer core-shell structure and can respond to CO2 partial pressure in the rumen gas production peak period to achieve pulse type rapid release of eucalyptus globulus essential oil, and methanogens are accurately inhibited; the sustained-release microsphere unit adopts functionalized polysaccharide composite microspheres to load cobalt acetate, can stably release cobalt element in rumen, and slowly and continuously releases cobalt element in abomasum and intestinal tract after passing the rumen. The two parts are compounded in a physical mode and do not interfere with each other, and space-time precise delivery and function coordination at different parts of the digestive tract are achieved. The method can effectively improve the methane emission reduction efficiency, improve rumen fermentation and improve the nutrient digestibility of the feed.

The application discloses a Yarrowia lipolytica capable of degrading cellulose and application of the Yarrowia lipolytica in improving rumen fermentation efficiency. The Yarrowia lipolytica strain (Yarrowia lipolytica LY6) has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2025327. The strain is separated and screened from soil around a fermented feed production enterprise, can degrade cellulose and grow in the range of 10-40 DEG C. The application aims at the deficiencies of conventional yeasts, such as the inability to directly degrade cellulose and the anaerobic energy consumption for producing alcohol when the conventional yeasts are used to improve the rumen fermentation rate. The new type of yeast feed additive developed by using the characteristics of the Yarrowia lipolytica, such as strong stress resistance, wide temperature adaptability, the inability to grow anaerobically and the inability to produce alcohol, has the advantages of remarkable effect, low cost and stable action, and has a good market prospect.

The invention provides a daily ration formula for improving fattening performance and meat quality of down producing goats and application of the daily ration formula, and belongs to the technical field of animal nutrition and feed. Corn straw and caragana microphylla are used for jointly replacing 40-60% of high-quality forage grass, 0.07-0.13% of artemisia ordosica crude polysaccharide is added, the average daily gain of the down producing goats is remarkably increased, the feed conversion ratio is reduced, meanwhile, the feed conversion rate is increased, and meanwhile the fattening performance and meat quality of the down producing goats are improved. The meat quality is improved, including reduction of muscle water loss rate and cooking loss, increase of meat color redness value and the like; besides, the formula also improves the digestibility of nutrient substances, especially the digestibility of crude protein, improves the rumen fermentation function, and improves the concentrations of rumen ammoniacal nitrogen and mycoprotein. Experiments show that the daily ration formula not only achieves the feeding effect similar to that of high-quality pasture, but also remarkably reduces the feeding cost, improves the economic benefit and provides a scientific basis for barn feeding breeding of down producing goats.

The invention relates to a feed bionic processing system for simulating a rumination whole digestion chain and a processing method. Comprising a control system, a dynamic bionic differential shearing-grinding all-in-one machine, a spraying device I, an image on-line detection system I, a feed bionic rubbing mixer, an image on-line detection system II, a spraying device II, a vibrating screen classifier, a belt conveyor, a material box, an elevator, a bidirectional servo sliding table, a torque sensor I, a torque sensor II and a stirring shaft. Through the arrangement of the dynamic bionic differential shearing-grinding all-in-one machine, the spraying device I, the feed bionic kneading and mixing machine, the vibrating screen classifier, the elevator and the spraying device I, the three physiological processes of shearing and grinding of a chewing system of the ruminant animal, fermentation and kneading of the rumen and rumination and screening of the reticulum are respectively and accurately simulated. The processed feed completely retains an effective fiber structure, and the physical form of the feed is closest to the physiological needs of animals, so that the rumen fermentation efficiency and the digestive tract nutrient digestion and absorption rate are greatly improved.

The invention relates to application of pseudomonas PSC001 in degradation of aflatoxin B1 and promotion of rumen fermentation, and aims to solve the problems of pollution of aflatoxin B1 in feed and poor ruminant rumen fermentation function at the same time. The invention discloses the new application of the pseudomonas PSC001 with the preservation number of CCTCC M 2023814 for the first time, namely degradation of aflatoxin B1 and promotion of rumen fermentation. The key technical means is as follows: the strain or the microbial inoculum thereof is applied to an AFB1 pollution system or daily ration of ruminants, and can realize efficient detoxification and growth promotion under specific conditions (such as 30-42 DEG C and pH 5.0-8.0) for 24-60 hours. The highest degradation rate of the strain to AFB1 can reach 65.20%, the yield of volatile fatty acid in rumen can be remarkably increased, a microflora is optimized, and the strain has the dual effects of efficient detoxification and rumen health improvement, and is high in safety and wide in application prospect.

This invention provides a rumen methane inhibitor and its application, belonging to the field of ruminant farming. The active ingredients of the rumen methane inhibitor of this invention include: *Lactobacillus reuteri* inoculum, *Lactobacillus gasseri* inoculum, *Lactobacillus casei* inoculum, amylase, phytase, xylanase, cellulase, and β-glucanase. The rumen methane inhibitor can effectively regulate the intestinal microbiota of dairy cows, promote the decomposition of fibrous material in the rumen, optimize the rumen fermentation environment, thereby controlling rumen function, reducing methane production, and improving the productivity of ruminants. The rumen methane inhibitor is safe and environmentally friendly, which is conducive to its application in ruminant farming, agriculture, and other fields, and has good practicality.

The invention belongs to the technical field of livestock feed, and particularly discloses cotton straw fermented feed as well as a preparation method and application thereof. The cotton straw fermented feed comprises the following raw materials in percentage by weight: 80-90% of cotton straw, 5-10% of apple pomace and 5-10% of corn bran. The invention discloses a cotton straw fermented feed as well as a preparation method and application thereof. The cotton straw fermented feed has good palatability and high protein content, and can optimize a ruminant rumen fermentation mode and improve the feed utilization rate.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The invention discloses a feeder cattle fattening feed and a preparation method thereof, and the feeder cattle fattening feed comprises the following components in parts by weight: corn straw, sassafras leaves, medicago sativa, myriophyllum spicatum, myotonin, feather meal, fishbone dust, calcium hydrogen phosphate, carrots, salt, soybean oil, a traditional Chinese medicine additive, betaine and cane sugar. The corn straw and the sassafras leaves provide crude fibers to promote rumen fermentation, the feather meal and the fish bone meal supplement high-quality protein, and the palatability is improved through a puffing process. The traditional Chinese medicine additive can significantly improve the serum immune globulin level and reduce common diseases such as cold and diarrhea. Calcium hydrogen phosphate promotes calcium and phosphorus absorption and improves skeletal development; the probiotics and the dietary fibers cooperate to regulate the intestinal microecology. The double-shaft extrusion puffing process shortens a raw material processing chain and reduces energy consumption, puffed particles are long in storage time, process data are recorded to a block chain to facilitate data tracking, and the method is suitable for being used in large-scale farms.

The invention provides efficient fiber degradation type prevotella and application thereof in promoting rumen fermentation, and relates to the technical field of microbiology, the prevotella is obtained by separating and purifying healthy Holstein cow rumen fluid, and is classified and named as Prevotella sp; the strain is preserved in Guangdong Microbial Culture Collection Center on June 20, 2025, and the preservation number is GDMCC66564; the prevotella SCX001 disclosed by the invention can efficiently degrade fibers in rumen fermentation liquor of the calves, and the strain can be used as a candidate probiotic for promoting intestinal health of ruminants such as dairy cows and the like; an in-vitro fermentation test verifies that the strain has a remarkable fiber degradation function, and a new thought and prospect are provided for applying the strain to the field of high-fiber feed to promote the growth of animals and maintain the body health of the animals in the future.

The invention provides a compound feed additive for ruminant rumen methane emission reduction and a verification method thereof, and relates to the technical field of feed additives and ruminant nutrition regulation and control. The compound feed additive is composed of nitroethane, fumaric acid and 3-nitrooxy propyl alcohol according to the molar ratio of 1: 1: 1, and effective action concentration is formed in rumen fermentation liquor. The method comprises the following steps: constructing a control group and a treatment group from basic ration, artificial saliva and rumen fluid by utilizing an in-vitro simulated rumen fermentation system, carrying out anaerobic culture at 39 DEG C for 24 hours, and then measuring the methane content, fermentation parameters and volatile fatty acid composition so as to evaluate the emission reduction effect. The compound feed additive disclosed by the invention can cooperatively regulate and control rumen hydrogen metabolism, keeps rumen fermentation stability while remarkably inhibiting methane generation, and provides a feasible technical approach for low-methane-emission feeding of ruminants.

The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.

This invention discloses a sheep feed, its preparation method, and a method for improving sheep production performance, relating to the field of animal husbandry technology. Feeding sheep with the sheep feed provided by this invention can significantly improve their production performance. Specifically, the rumen-protected additive has minimal impact on rumen fermentation and is easily digested, absorbed, and utilized by the rumen's post-digestive system. It also increases the energy concentration of the feed, meeting the energy needs of ruminants. Palm oil fat powder can effectively improve body fat distribution, enhance animal slaughter and reproductive performance, and alleviate heat stress. Rumen-protected glucose can rapidly increase blood glucose concentration, reduce ketone body levels, alleviate negative energy balance, improve animal production performance, and improve milk and meat quality. Furthermore, the feed provided by this invention is free of antibiotics and hormones, improves mutton quality, increases economic benefits, and is safe and harmless.

The invention provides a liquid-state fermentation concentrate supplement as well as a preparation method and application thereof, and belongs to the technical field of feeds. After the liquid-state fermentation refined supplementary feed replaces a part of complete feed in a lactating cattle formula, the milk yield of the dairy cattle and the feed conversion rate are remarkably increased; rumen fermentation parameters and micro-ecological zones are regulated and controlled, and the relative abundance of partial protozoa and methanogens is reduced, so that the yield of greenhouse gas is reduced; the yield of beneficial volatile acids and metabolites in intestinal tracts of dairy cows is increased, the score of excrement of lactating cows is improved, and diarrhea is relieved.

The invention provides a method for synergistically degrading lignin through compound bacteria-compound enzyme and application, and relates to the technical field of biological fermentation feed. The preparation method comprises the following steps: mixing corn straw, caragana microphylla, salix mongolica, bran and soybean meal in proportion, crushing, sterilizing at 121 DEG C, regulating the water content and pH value, inoculating a complex microbial inoculum consisting of pandoraea, bacillus thiamine and white-rot fungi, spraying a complex enzyme preparation consisting of laccase, xylanase and manganese peroxidase, and performing staged fermentation at 45-60 DEG C to obtain the high-yield organic fertilizer. The method realizes efficient degradation of lignin, cellulose and hemicellulose, increases the content of crude protein and crude fat, improves the rumen fermentation condition and meat quality of mutton sheep, and breaks through the limitations of low degradation efficiency and poor adaptability in the prior art.