The invention relates to the technical field of dog feed, in particular to feed for improving disease resistance of meat dogs and a preparation method. The feed for improving the disease resistance of the meat dogs is prepared from, by weight, 20-30 parts of corn, 25-35 parts of brown rice, 15-20 parts of peanut cakes, 12-18 parts of fermented soybean meal, 8-12 parts of vinasse, 10-15 parts of bone meal, 6-10 parts of beef powder, 8-10 parts of chicken powder, 8-12 parts of pig liver powder, 3-5 parts of lettuce, 3-4 parts of celery, 3-4 parts of cauliflowers, 2-3 parts of white gourds, 2-4 parts of alfalfa sprouts, 6-8 parts of sweet potato leaves, 4-6 parts of fructus hippophae, 10-12 parts of tomato pomace, 2-3 parts of lentinula edodes powder, 4-6 parts of celosia cristata flowers, 3-4 parts of radix astragali, 2-3 parts of radix codonopsis, 2-3 parts of yeast powder, 1-2 parts of cod liver oil, 1-2 parts of salt and 1-2 parts of additive. The preparation method mainly comprises the steps of raw material weighing, mixing, fermentation and the like. The feed is reasonable and practical in formula, comprehensive in nutrition, good in palatability and easy to digest, the immunity of the meat dogs is enhanced, the disease resistance of the meat dogs is improved, and the meat dogs can grow quickly.