60 results about "Leymus" patented technology

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.

The invention provides a shrub and grass matched wind prevention and sand fixation method under a photovoltaic panel of a mudshandack sand land. The method comprises the following steps: S1, planting herbaceous plants matched with small half shrubs in front of the photovoltaic panel; s2, adopting sand-barrier automatic sowing equipment to plant shrubs and weeds in and behind the photovoltaic panel; the herbaceous plants comprise artemisia desertorum, agriophyllum squarrosum, liquorice, wheatgrass, leymus chinensis and astragalus mongholicus in North China; the small half shrubs are caragana microphylla, caragana angustifolia and camellia hupehensis; the shrubs are red sand, caragana microphylla, caragana angustifolia, amygdalus pedunculata, alpinia hupehensis, salix babylonica, artemisia stelleriana, caragana microphylla and thyme; the weeds are agriophyllum squarrosum, agriophyllum squarrosum, pugionium Wind prevention and sand fixation engineering and photovoltaic industry land are ingeniously combined, adaptive vegetation is planted under the photovoltaic panel which is most prone to wind erosion and in the peripheral area, surface soil can be effectively fixed, sand dune mobility is reduced, a photovoltaic panel support foundation is protected against wind erosion damage, and the service life of a power station is prolonged.

The invention relates to the technical field of plant genetic engineering, and provides application of Leymus chinensis LcCBP60b gene in regulation and control of plant tiller number and biological yield. It is found for the first time that the LcCBP60b gene can improve the tiller number and the biological yield of leymus chinensis, and the LcCBP60b gene has important guiding significance on cultivation of high-yield and high-quality leymus chinensis varieties; the invention also clarifies the molecular mechanism of the LcCBP60b gene in regulation and control of the tiller number and the biological yield of Leymus chinensis, further analyzes the molecular module for regulating and controlling the tiller number and the biological yield of plants, can enrich the corresponding molecular mechanism, and provides a new breeding direction for breeding high-yield and high-quality varieties.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of the SNP molecular marker combination. The SNP molecular marker combination is composed of five SNP molecular markers, the SNP molecular markers in the group are located at the 80176348th site of a chromosome 6 of elymus dahuricus, the 490854164 site of a chromosome 9 of elymus dahuricus, the 291625249 site of a chromosome 11 of elymus dahuricus and the 64884340th site and the 65147058 site of a chromosome 12 of elymus dahuricus, base polymorphism CC / CG (GG), TT / TG, AA / TT, AA / GG and TT / CC exist at the sites, CC-TT-AA-AA-AA-TT is a saline-alkaline tolerant genotype, and CG / GG-TG-TT-GG-CC is a saline-alkaline sensitive The SNP molecular marker combination disclosed by the invention is used for detecting the saline-alkaline tolerance of the elymus dahuricus, is accurate and reliable, and effectively improves the identification efficiency of the germplasm and variety of the saline-alkaline tolerance elymus dahuricus.

The invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing, which comprises the following steps: equally dividing a target land into a plurality of grid areas, collecting soil samples in different grid areas to measure the pH and total nitrogen content of soil, and dividing the soil into an ecological restoration area, a forage grass production area and an economic plant area according to the measured pH and total nitrogen content of the soil. Suaeda glauca and sesbania are planted in the ecological restoration area, alfalfa and leymus chinensis are planted in the forage grass production area, radix isatidis and purple perilla are planted in the economic plant area, and crop rotation planting is conducted on the three areas with two years as the cycle. According to the design, through complementation of plant communities, the fertilization requirement can be reduced, the soil remediation speed can be increased, grassland rotation management of multi-subject land sharing is achieved through ecological function complementation, the land utilization efficiency can be effectively improved, the grassland production-ecology-life three-generation functions are considered, and sustainable development of the saline-alkali grassland is promoted.

The application discloses a method for promoting the absorption of methane in sheep grassland, and belongs to the field of environmental protection.The method is achieved by the following steps: applying nitrogen fertilizer to artificial sheep grassland in the growing season (June-September) to promote the absorption of methane in the grassland, so as to achieve the purpose of methane emission reduction.The nitrogen fertilizer added in the method is ammonium nitrate, the adding frequency is once a month, and the total dose is 100 kg N / hm 2 . Through the method, the total absorption amount of methane in the grassland in the growing season and the next spring freeze-thaw period is increased by more than 140% compared with the natural condition. The method is simple to operate and low in cost, and is helpful to the realization of the low-carbon target in the management process of artificial sheep grassland. In particular, in the next spring freeze-thaw period, the average value of the nitrogen application group is 7.1 mg m ‑2 , the average value of the nitrogen-free group is-5.0 mg m ‑2 , and the nitrogen application treatment increases the cumulative absorption amount of CH4 in the grassland soil in the spring freeze-thaw period by 240.4%.

This invention discloses a method for synergistically improving grassland plant diversity and productivity, relating to the field of degraded grassland ecological restoration technology. The method involves selecting degraded Leymus chinensis meadow steppe as the restoration target and delineating restoration plots. Within the operational window, targeted aeration and fertilization are carried out on the restoration plots to obtain improved restoration plots. These improved restoration plots are then continuously fenced off for at least two growing seasons for natural enclosure. This invention, through the synergistic effect of targeted aeration and precise fertilization, breaks through soil physical limitations and replenishes nutrient bottlenecks, achieving simultaneous improvement in plant diversity and productivity of degraded Leymus chinensis meadow steppe. It effectively optimizes the plant community structure and promotes the evolution of degraded communities into stable perennial herbaceous communities. Furthermore, this invention features highly standardized and mechanized operational parameters, high restoration efficiency, and is suitable for the large-scale restoration needs of degraded Leymus chinensis meadow steppe, balancing ecological benefits and production potential, and has broad application prospects.

The application discloses application and a method of 1-Kestose in improving drought resistance of plants, and the drought resistance of plants is improved by using a 1-Kestose spraying agent, wherein the effective concentration of the leaf spraying agent is 0.1-10 muM, the optimal concentration of alfalfa is 10 muM, the concentration of Leymus chinensis, wheat and tall fescue is 1 muM, and the concentration of corn, cotton and ryegrass is 0.1 muM. The continuous leaf spraying is performed when the crops are two weeks old, and then drought stress treatment is performed. The drought resistance of crops can be significantly improved by externally applying trace 1-Kestose, and the method has the advantages of low concentration, simple operation, low cost, environmental friendliness and the like, and has a wide application prospect in drought-resistant agricultural production.

The invention discloses a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in a field, belongs to the field of crop genetic breeding, and particularly relates to a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in the field. In order to solve the problem of large character data error caused by initial non-uniformity and non-uniformity of transplanting damage of leymus chinensis tillering seedlings in existing field evaluation, the method comprises the following steps: 1, carrying out first homogenization treatment; 2, transplanting for the first time; 3, performing homogenization treatment for the second time; 4, transplanting for the second time; 5, observing a saline-alkaline tolerance phenotype; 6, evaluating saline-alkaline tolerance of the saline-alkaline tolerant plant germplasm. The core of the method is that two times of homogenization treatment and two times of transplanting are carried out on leymus chinensis tillering seedlings, the initial uniformity of test plants is greatly improved through the process, and systematic errors caused by the transplanting link are effectively controlled.

The method for saline-alkali land remediation based on simultaneous activation of Leymus chinensis rhizome and sexual complementary sowing in different layers belongs to the technical field of ecological remediation and sustainable utilization of grassland. The method solves the technical problem that there is currently a lack of a collaborative remediation technology that can combine low disturbance, activation of the original Leymus chinensis vegetative propagation potential, and efficient introduction of sexual reproduction. The method comprises the following steps: S1, determining a saline-alkali region that needs to be remediated; S2, determining a time window for remediation operation; S3, implementing narrow-width low-disturbance deep ploughing, and leaving a width between adjacent deep ploughing strips that is greater than the width of the deep ploughing narrow strips; S4, implementing in-situ collaborative seeding and directional root induction, and synchronously implementing precision seeding, positioning fertilization and habitat directional induction in the narrow strips where deep ploughing is completed by using a mechanical linkage device; and S5, field management.

The invention discloses a leymus chinensis planting integrated operation cooperation system applying intelligent control, and relates to the technical field of intelligent agriculture, and the system comprises a cloud server which is in communication connection with the following modules: a perception decision cooperation module which is used for comprehensively collecting soil parameters to construct a soil attribute spatial distribution map, and sending the soil attribute spatial distribution map to the cloud server; and a rotary tillage strategy is optimized by adopting cloud collaboration and a fruit fly algorithm, and the rotary tillage depth is determined so as to carry out adaptive regulation and control on the rotary tillage unit. A high-precision soil attribute spatial distribution diagram is constructed by collecting soil parameters, an optimal pressing depth combination of each rotary tillage unit is quickly calculated by using a fruit fly algorithm, and an instruction is issued to dynamically adjust a hydraulic execution mechanism, so that the limitation of a traditional mechanical fixed operation mode is broken through; an optimal soil physical environment is created for healthy growth of leymus chinensis roots, and the problem that the roots are difficult to penetrate through hard soil layers due to high spatial heterogeneity of soil hardness is fundamentally solved.

The invention provides a method for recovering grassland between photovoltaic panels, and belongs to the technical field of grassland recovery. The recovery method comprises the steps that during soil preparation and deep scarification, crop waste is thrown, and an organic interlayer is constructed; meanwhile, base fertilizer is applied during soil preparation and deep scarification; the deep scarification depth ranges from 30 cm to 40 cm; before sowing, microbial fertilizer treatment is carried out, pasture mixed seeds are sown, and the pasture mixed seeds are northeast leymus chinensis, puccinellia koreana and elymus dahuricus. According to the method, through the synergistic effect of the northeast Chinese wildrye, the puccinellia koreana and the elymus dahuricus and the methods of organic interlayer salt resistance, fertilization, microbial fertilizer treatment and the like, the vegetation coverage, community density and overground biomass of the grassland between the photovoltaic panels are remarkably improved, the alkali spot area proportion of the grassland between the photovoltaic panels and the pH value of 0-20 cm surface soil are reduced, and compared with the current sowing year, the method has the advantages that the cost is reduced, and the yield is increased. The emergence rate of the pasture in the second year of sowing, the fresh weight of the overground plants and the dry weight of the overground plants are remarkably increased, and the recovery efficiency and ecological benefits of the grassland between the photovoltaic panels are improved.

The present invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing. The target plot is first divided into multiple grid areas. Soil samples are collected from different grid areas to determine the soil pH and total nitrogen content. Then, based on the measured soil pH and total nitrogen content, the area is divided into an ecological restoration area, a forage production area, and an economic plant area. Salicornia and Sesbania are planted in the ecological restoration area, alfalfa and Leymus chinensis are planted in the forage production area, and Isatis indigotica and Perilla are planted in the economic plant area. Crops are rotated in the three areas on a two-year cycle. The above design can reduce fertilization requirements and increase soil remediation speed through the complementarity of plant communities. It can achieve multi-subject land sharing grassland rotation management through the complementarity of ecological functions, effectively improve land use efficiency, take into account the "three lives" of grassland production, ecology, and life, and promote the sustainable development of saline-alkali grassland.

The invention discloses a method for increasing the germination rate of leymus chinensis seeds by using a plant growth regulator fluridone, and belongs to the technical field of leymus chinensis cultivation. The method provided by the invention comprises the step of treating ears of leymus chinensis plants by using a fluridone solution. According to the method, the fluridone solution is applied in the ear development process and directly acts on the seed development process, so that the fluridone can be more effectively absorbed and utilized by the seeds, and the germination rate is promoted to be increased; the fluridone enters the seeds through a natural physiological process, can be uniformly distributed at ears, and can improve the physiological status of the seeds in a seed formation stage, so that the subsequent germination rate is increased; extra treatment after harvesting is not needed, and time and labor are saved. Compared with a method for directly treating the seeds by using an agent after the seeds are harvested, the method has the advantages of more efficient absorption, uniform distribution, improvement of the seed quality in advance, simplification of subsequent steps and the like, and the physiological status and germination potential of the seeds can be more effectively improved.

This invention discloses a shallow-sowing drip irrigation process for Leymus chinensis cultivation, belonging to the field of seedling cultivation. The invention first determines the drip irrigation coefficient for each seedling cup by observing the growth status of Leymus chinensis and using this growth status as a constraint. Based on the drip irrigation coefficient, the growth status of Leymus chinensis in the current seedling cup is determined. When the drip irrigation coefficient of the seedling cup is greater than the target drip irrigation coefficient, the Leymus chinensis is considered to be growing vigorously; otherwise, the growth is considered abnormal. If abnormal growth is detected, drip irrigation is first performed at a baseline water supply, and then the drip irrigation coefficient is continuously assessed. If the drip irrigation coefficient of the next cycle is less than or equal to the current drip irrigation coefficient, drip irrigation is stopped to avoid water waste. Furthermore, when the Leymus chinensis in the seedling cup is determined to be growing vigorously, the water supply coefficient of the seedling cup is determined based on the soil moisture and the temperature and humidity of the seedling room. The water supply coefficient and the drip irrigation coefficient of the seedling cup are used as control factors.

The present invention discloses a substance, method, and application for detecting multiple insertion / deletion polymorphisms in Leymus chinensis, belonging to the technical field of assays or detection methods involving enzymes, nucleic acids, or microorganisms. This application can be used to identify or assist in the identification of Leymus chinensis fruit set rate using 30 primer pairs, from InDel-1-P to InDel-30-P. These 30 primer pairs can accurately and efficiently identify varieties with this trait, facilitating the identification and screening of high-fruit-set Leymus chinensis germplasm during the seedling stage by breeders, accelerating the selection of new Leymus chinensis varieties.

The invention discloses a saline-alkali soil remediation method based on leymus chinensis rhizome activation and sexual reseeding synchronous different layers. Belongs to the technical field of ecological restoration and grassland sustainable utilization. The technical problem that at present, a synergistic remediation technology which can be combined with low disturbance, activate the asexual propagation potential of the native leymus chinensis and efficiently introduce sexual propagation is lacked is solved. The method comprises the following steps: S1, determining a saline-alkali land area needing to be repaired; s2, determining a time window for repairing operation; s3, narrow low-interference deep scarification is implemented, and the reserved width between every two adjacent deep scarification strips is larger than the width of a deep scarification narrow strip; s4, implementing in-situ collaborative reseeding and directional root guiding of the seeds, and synchronously implementing precision seeding, positioned fertilization and habitat directional induction by using a mechanical linkage device in a narrow strip after deep scarification; s5, field management.

The invention belongs to the technical field of biology, and discloses an application of an LcCBP60b-LcNAC2-LcALY2 molecular module in regulating and controlling related traits of the maturing rate of leymus chinensis. The invention provides and verifies that the molecular module LcCBP60b-LcNAC2 regulates the transcription of LcALY2, and further regulates the formation of the setting rate character of Leymus chinensis. Compared with a wild type, the LcCBP60b silent transgenic plant has the advantages that the maturing rate is obviously increased, the ear length and the spikelet number are obviously increased, in the LcCBP60b silent transgenic plant, the transcription level of the LcNAC2 gene is reduced, and the inhibition effect of an LcNAC2 transcription factor on LcALY2 is reduced. The regulation and control change is helpful to stimulate the transmission of flowering and seed development related signals in the leymus chinensis, so that the maturing rate of the leymus chinensis is improved. The invention lays a technical foundation for improving the maturing rate of leymus chinensis and cereal crops through molecular design breeding.

The application provides application of LcSFC6 protein and related biological materials thereof. The amino acid sequence of the LcSFC6 protein is shown as SEQ ID No: 2. The application takes wild Leymus chinensis as a receptor material to obtain LcSFC6 The overexpression plant of the gene. Compared with the wild type, LcSFC6 The stress resistance of the overexpression plant is significantly improved. The LcSFC6 and the coding gene thereof have important theoretical and practical significance for cultivating new varieties of Leymus chinensis and other plants with improved stress resistance, and can be applied to cultivation and identification of plant varieties with resistance required by agriculture, animal husbandry and ecological environment management, and have high practical application value. The application has wide application prospect in the field of agriculture.

This application provides a method and apparatus for determining the nitrogen fertilizer application rate for *Leymus chinensis* forage. The method includes: collecting growth data and biological samples of target *Leymus chinensis* forage within a target area; determining the aboveground dry matter, belowground dry matter, and nitrogen concentration data of the target *Leymus chinensis* forage using the biological samples; constructing a first critical nitrogen dilution curve based on the aboveground dry matter and nitrogen concentration data; and determining the nitrogen fertilizer application rate for the target area based on the belowground dry matter, the first critical nitrogen dilution curve, and the growth data. This method enables precise application of nitrogen fertilizer during the production of *Leymus chinensis* forage, ensuring yield and root growth while reducing fertilization costs and environmental pollution.

This invention relates to a chromosome-specific ND-FISH probe of *Leymus chinensis* and its applications and kits, belonging to the field of molecular genetics. The *Leymus chinensis* chromosome-specific ND-FISH probe of this invention has a nucleotide sequence of 5'-CAATGGGCCTCTACTATAGGGTGTTAGGCTCACACTAGAGAA-3', with a fluorescent label at the 5' end. This *Leymus chinensis* chromosome-specific ND-FISH probe can efficiently distinguish between wheat and *Leymus chinensis* chromosomes within four hours through non-denaturing fluorescent in situ hybridization, greatly improving the screening efficiency of wheat-*Leymus chinensis* derived progeny, reducing experimental costs, and laying the foundation for improving wheat breeding using the closely related species *Leymus chinensis*, thus broadening wheat genetic diversity.

The invention belongs to the technical field of molecular marker breeding, and particularly relates to a method for obtaining a new selfing and fruiting variety of leymus chinensis, a molecular marker LcDUF247II-S3 and application of the molecular marker LcDUF247II-S3.

The invention discloses a desulfurized gypsum and fly ash experimental device and method for mining area leymus chinensis vegetation reconstruction, and relates to the technical field of ecological restoration, the desulfurized gypsum and fly ash experimental device comprises a mounting cover, a mixing box, a stirring mechanism, a flapping mechanism, a conveying belt, a placing box, a culture basin, an air freezing and thawing testing machine, a detection machine, a data processing end and a cleaning machine, the rear end of the top in the mounting cover is fixedly connected with a mixing box, a stirring mechanism is arranged, through rotation and movement of two sets of weak direct-current electric field mechanisms and work of an ultrasonic atomization spray head, material caking is broken, mixing dead corners are eliminated, the uniformity of matrixes with different proportions is accurately regulated and controlled, meanwhile, materials penetrate, dead-corner-free infiltration is achieved, and the mixing efficiency is improved. The interface combination of the mineral substances, the organic matters and the biological bacterial liquid is enhanced, the matrix stability and the nutrient effectiveness are improved, and a uniform and high-activity mixed system is provided for the construction of the ternary composite artificial soil.

The invention discloses a phenology-based leymus chinensis community distribution remote sensing identification method. The method comprises the following steps: step 1, obtaining a time sequence remote sensing image; 2, analyzing an NDVI time sequence curve; step 3, determining an effective time period; step 4, constructing a classification sample set; 5, constructing a classification feature set; step 6, remote sensing image classification; according to the method, the NDVI mutation time period caused by human factors in the NDVI time sequence curve is eliminated, and the effective time period of leymus chinensis community identification is determined, so that the accuracy of data is ensured; a dynamic time warping algorithm is used for evaluating the similarity between a time sequence curve of a pixel position and a time sequence curve of a typical sample point, sample amplification is completed, and a more representative classification sample set is constructed; a classification feature set is constructed by combining and integrating typical phenological parameters and common image features, so that the classification result is more accurate, and efficient and accurate recognition of the leymus chinensis community based on the random forest algorithm is realized.

The invention discloses a method for obtaining Leymus chinensis and New Wheat Straw distant hybridization offspring using young embryo rescue technology, comprising the following steps: 1) hybridization: Leymus chinensis is as female parent, and New Wheat Straw is as male parent; Collect male parent pollen, pollen is pointed on female parent pistil stigma with tweezers for pollination, and immediately bag; 2) disinfection: take hybrid spike after pollination, strip out non-aborted ovary, use volume fraction 75% alcohol to soak 1min, sterile water rinse three times, sodium hypochlorite soak 10min, rear sterile water rinse 5 times; 3) use one-step seedling method or induced callus to expand and multiply, differentiate into seedling method seedling; 4) practice seedling transplanting: when tissue culture seedling grows to 10cm 15cm, ultrapure water is added in culture bottle, after indoor seedling hardening 7d, plant root culture medium is rinsed clean, transplanted into sterilized nutrient soil and cultivated. The present invention has higher young embryo germination rate, induced callus rate and differentiation rate, obtains the hybrid offspring of Leymus chinensis and New Wheat Straw in a relatively short time.

The invention discloses a preparation method and application of a multi-element synergistic compost material for repairing heavily-degraded grassland, and relates to the technical field of ecological restoration of the heavily-degraded grassland. According to the preparation method and application of the multi-element synergistic compost material for repairing the severely degraded grassland, the compost period is shortened to about 35 days by optimizing the compost raw material ratio, the fermentation process and the turning strategy, and compared with the effective period of 3-5 years in the prior art, the early-stage effect development speed of repairing the degraded grassland is greatly increased, and the cost is reduced. After the compost material is applied, the physical and chemical environment of soil can be quickly improved, and suitable conditions are provided for vegetation growth. Meanwhile, a multi-element synergistic composting system and a scientific application method form resultant force, so that the soil structure of the severely degraded grassland is effectively improved, the soil bulk density is remarkably reduced, the pH value is close to neutral, the salt content is greatly reduced, the comprehensive quality index of the soil is remarkably improved compared with a control group, and the survival rate and the growth index of leymus chinensis are further remarkably improved.

This invention relates to the field of biotechnology, specifically to the application of bioproducts expressing the EsPTI15 gene to regulate tiller number. The nucleotide sequence of the EsPTI15 gene is shown in SEQ ID NO:1. This invention is the first to discover that, compared to wild-type *Leymus chinensis*, EsPTI1… 5 Overexpression of the EsPTI15 gene significantly increased the number of tillers in *Triticum aestivum*, confirming that overexpression of the EsPTI15 gene positively regulates tillering development. This finding not only enriches the molecular mechanism theory of plant tillering regulation but also demonstrates the potential of the EsPTI15 gene to increase the number of tillers, paving the way for subsequent gene manipulation to introduce EsPTI1. 5 The research and development of technologies that increase the number of tillers in forage plants and thus improve biomass has provided key theoretical support and genetic resources.

The present invention relates to the technical field of image processing, and in particular to a method for obtaining a seed setting rate of Leymus chinensis, an electronic device, and a storage medium. The method comprises: obtaining a Leymus chinensis spike X-ray image corresponding to a Leymus chinensis sample, performing pixel segmentation processing on the Leymus chinensis spike X-ray image based on a target pixel segmentation threshold, obtaining a binarized image corresponding to the Leymus chinensis spike X-ray image, determining a plurality of Leymus chinensis spike regions and a plurality of Leymus chinensis scattered grain regions from the binarized image, performing iterative image pixel segmentation on the Leymus chinensis spike regions and the Leymus chinensis scattered grain regions, obtaining a plurality of second seed regions, and then obtaining a plurality of target floret regions according to the binarized image, determining the ratio of the number of the second seed regions to the number of the target floret regions as the single plant setting rate corresponding to the Leymus chinensis sample, and thereby obtaining the Leymus chinensis seed setting rate. The present invention can automatically, rapidly, and with high throughput identify Leymus chinensis seeds and florets, thereby realizing accurate and rapid acquisition of the Leymus chinensis seed setting rate.