128 results about "Leymus" patented technology

The invention discloses a method to promote the rejuvenation and plant growth of leymus chinensis, which is characterized in that, during the turning green period or before the rains on the growth declination period of leymus chinensis, root cutting operation is performed to leymus chinensis grass lawn. The method has the advantages that, permeability is improved by cutting the roots, and the high yield of leymus chinensis is maintained; in addition, during the root cutting operation, the vegetative soil structure of the leymus chinensis is loosen and improved, and the soil hardening factor restricting the vegetative propagation of leymus chinensis is eliminated; therefore the permeability of the soil is improved; good environment is created for grassland vegetation growth, and the productivity of grasslands is improved; the method is suitable for the ecological restoration and management of naturally degraded leymus chinensis grass lawn; the service life of artificial leymus chinensis grass lawn is prolonged, and leymus chinensis with good quality is provided.

An organic sheep feed for improving the quality of mutton is prepared by using the following raw materials, by weight, 100-120 parts of bur clover, 100-120 parts of Leymus chinensis, 70-80 parts of grapefruit peel, 300-320 parts of corn, 80-90 parts of corn germ meal, 5-6 parts of medicated leaven, 6-7 parts of malt, 4-5 parts of radish seed and 3-4 parts of Rangooncreeper Fruit. The addition of the grapefruit peel and the corn germ meal added to the organic sheep feed for improving the quality of mutton makes the feed have abundant nutrition and fragrant taste, and the addition of powders of medicated leaven, malt, radish seed and Rangooncreeper Fruit makes increases the daily weight increase of mutton sheep.

The invention discloses a special feed for meat donkeys and a preparation method of the special feed. The special feed comprises the following formula components in parts by mass: 25-27 parts of soybean straw, 21-23 parts of paddy rice, 14-18 parts of peas, 12-16 parts of wheat bran, 11-13 parts of rice bran, 9-12 parts of mung bean meal, 8-10 parts of carrot tops, 9-13 parts of potatoes, 6-9 parts of a cottonseed cake, 5-8 parts of bone meal, 6-9 parts of dry leymus chinensis, 7-10 parts of ryegrass, 3-5 parts of lysine, 2-6 parts of pumpkin vine powder, 1-4 parts of fennel seed powder, 2-4 parts of common salt and 50-70 parts of water. The special feed disclosed by the invention is simple in preparation, low in cost, balanced in nutrients and scientific in mixture ratio, provides various nutritional substances required by growth of the meat donkeys and enhances the disease resistance of the meat donkeys.

The invention belongs to the planting technique field, which is a chinensis germination method of combined low temperature seed soaking and temperature changing. The invention mainly comprises two parts of partially releasing the chinensis seed dormancy by the seed soaking method in the seed pretreatment technique and rapidly increasing the seed germination rate of chinensis by applying the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) low temperature seed soaking, 3) seed disinfection and 4) variable temperature germination. The invention can increase the chinensis germination rate from 56.7 percent to above 88.7 percent compared with the chinensis seed treatment by a single method. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.

The invention discloses a method for reseeding alfalfa in degenerated leymus chinensis pasture. The method comprises the following steps: reseeding alfalfa into natural pasture, and performing fertilization and pasture management of clipping at regular time, so as to maintain the ratio of the alfalfa in the natural pasture. Tests show that the pasture reseeding technique is simple to operate, and materials for reseeding, fertilizers and the like are wide in source, simple in production cost, low in cost and applicable to large-scale popularization. By adopting the reseeding technique disclosed by the invention, not only are productivity and forage grass quality of the degenerated leymus chinensis pasture improved, but also the nutrients of nitrogen in pasture soil are increased, utilization and circulation of the nitrogen in a pasture ecological system are promoted, diversity and stability of a pasture system are maintained, and technical supports are provided to establish high-yield stable natural pasture. The method has very high economic and ecological values, relatively high practicability and generalizability, and very wide application prospects.

The invention relates to a leymus chinensis cutting device, in particular to an equidistant cutting device for leymus chinensis in animal husbandry. The equidistant cutting device, provided by the invention, for the leymus chinensis in the animal husbandry can cut equidistantly and is easy to operate and high in cutting efficiency. In order to solve the technical problem, the equidistant cutting device, provided by the invention, for the leymus chinensis in the animal husbandry comprises a bottom plate, a left side plate, a first sliding rail, a first pulley, a right side plate, an L-shaped rod, a guide rod, a first connecting rod, a second connecting rod, a motor, a first pawl, a lifting rod, a fixed stop block, an elastic piece, a blade, a contact wheel, a second pawl, a second spring, a pulling line, a pulling ring, a first sliding block, a first spring, a placement frame, a second sliding rail, a second sliding block, a double-sided ratchet, a second pulley, a pulling rope and a third spring. The equidistant cutting device for the leymus chinensis in the animal husbandry is driven by the motor; the double-sided ratchet can move only a short distance every time by rotating the motor anticlockwise, and the movement distances of each time are equal.

The invention provides a direct-sowing seedling culture method capable of increasing survival rate of leymus chinensis growing in moderate-severe soda saline-alkali soil and relates to a direct-sowing seedling culture method of the leymus chinensis in order to solve the problems of low germination rate, low seed emergence rate and low transplanting survival rate of the leymus chinensis in a conventional leymus chinensis direct-sowing seedling culture technology under the background of moderate-severe saline-alkali soil. The direct-sowing seedling culture method comprises steps as follows: 1, impurity removal and soaking of leymus chinensis seeds; 2, sieving of saline-alkali soil for seedling culture; 3, selection of culturing vessels; 4, filling of the culturing vessels with the saline-alkali soil; 5, trenching on the upper surface of moderate seedling culture soil and addition of isolation media; 6, sowing; 7, management after sowing. According to the direct-sowing seedling culture method, the leymus chinensis seeds can be isolated from the severe saline-alkali soil stress environment in the germination period and the acicular seedling period, a better environment is provided for growth of the leymus chinensis, and the germination rate of the leymus chinensis and the survival rate of the seedlings are increased to the great extent; the germination rate of the leymus chinensis reaches 80% or higher, and the survival rate reaches 90% or higher. The method is suitable for direct-sowing seedling culture of the leymus chinensis.

The invention relates to an efficient germination method of leymus chinensis seeds and aims to solve the technical problems that experiment varieties for germination of leymus chinensis seeds are limited to bred single varieties, have poorer data comparability, are time-consuming and effort-consuming and have no repeatability at present. The method comprises the steps as follows: 1, seed selection; 2, wall breakage and ultrasonic treatment; 3, preparation of a culture medium; 4, soaking with an ethanol aqueous solution and sterilization with sodium hypochlorite; 5, culture in the culture medium. The method has the advantages as follows: 1) germination of leymus chinensis can be not limited by season and environment and has the advantages of being simple, convenient, quick, efficient, seldom restricted by varieties and the like; 2) dormancy of the leymus chinensis seeds is broken through effective combination of physical, chemical and hormone for the first time, and the germination rate of the leymus chinensis is significantly increased, so that sufficient seedlings can be obtained to be applied to establishment of a leymus chinensis regeneration system and a genetic transformation system and commercial production of pasture.

The present invention discloses a Chinese wildrye levan hydrolase, and an encoded gene and an application thereof. The albumen is Chinese wildrye levan hydrolase, which is the protein having one of the following amino acid residue sequence: 1) the amino acid residue sequence of the sequence 1 in the sequence table; and protein that is obtained by substituting and/or deficiency and/or addition of one or more amino acid residue to the amino acid residue sequence of the sequence 1 in the sequence table and has [beta]-2,1 glycosidic bond hydrolase function. The encode gene of the low temperature proof albumen can be transferred to a plant to improve the stress-resistance of the plant. The gene has a significant theoretical and practical significance for the research of the stress-resistance molecular mechanism of plant and the breeding of stress-resistance varieties of plant. The gene provides an economical, fast and effective approach for improving the stress-resistance of crop. The gene has wide applications and market prospect in the agriculture field.

The invention is a chinensis germination method of combined high temperature seed soaking and temperature changing, which belongs to the planting technique field. The invention mainly comprises two parts of partially releasing the chinensis seed dormancy by utilizing the seed soaking method in the seed pretreatment technique and rapidly increasing the seed germination rate of chinensis by applying the variable temperature technique. The invention concretely has four processes of 1) seed selection and impurity removal, 2) high temperature seed soaking, 3) seed disinfection and 4) variable temperature germination. The invention can increase the chinensis germination rate from 56.7 percent to above 84.0 percent compared with the chinensis seed treatment by a single method. Therefore, the invention has significant practical meaning and broad application value to both the chinensis seed cultivation and experimental study.

The invention discloses a leymus chinensis accelerant and an application thereof, and belongs to the technical field of biology. The leymus chinensis accelerant provided by the invention comprises nitrogen elements, phosphorus elements and brassinolide in the mass ratio of the nitrogen elements to the phosphorus elements to the brassinolide being 10000 to (1000-2500) to (0.0001-0.0003). The leymus chinensis accelerant is used for restoring degraded leymus chinensis grasslands. The leymus chinensis accelerant disclosed by the invention can quickly increase the proportion of leymus chinensis in community by promoting tiller differentiation of the leymus chinensis, so as to restore the degraded leymus chinensis grassland.

The invention discloses a Leymus chinensis gamogenesis capability improving method comprising the following steps: 1, applying mixed fertilizer on a Leymus chinensis meadow in a Leymus chinensis after-fruit nutrition period, and watering the Leymus chinensis meadow so as to dissolve the fertilizer in the soil; 2, burning the Leymus chinensis in spring the next year, and maintaining soil water content at 20-35% in a Leymus chinensis turning green period; 3, spraying water solution, mixing heteroauxin with naphthalene acetic acid, on the Leymus chinensis meadow when the Leymus chinensis grows to a jointing period, and keeping spraying until the flowering stage of the Leymus chinensis; 4, spraying water solution, containing 5-amino-laevulic acid and potassium nitrate, on the flowering Leymus chinensis when the Leymus chinensis grows to the full flowering stage, and keeping spraying until the Leymus chinensis ripening state. The method couples several artificial means so as to improve Leymus chinensis heading rate, ripening rate, and seed germination capacity, thus solving the problems that conventional research can only improve one index of three low indexes of the Leymus chinensis.

The invention discloses a germination method of Leymus chinensis seeds, relating to a seed germination method, and solving the technical problems of long dormancy period and low germination rate of Leymus chinensis seeds. The method comprises the following steps of: 1, soaking shelled Leymus chinensis seeds in sterile water at 5 DEG C and in a dark condition, disinfecting the seeds with ethanol and NaClO solution, washing the seeds with sterile water, and sowing the seeds in an MS culture medium; 2, culturing for 16 hours in the dark condition and at 28 DEG C, then culturing for 8 hours at 22 DEG C, and then circulating by taking 24 hours as a cycle; and 3, germinating for 2-14 days in the conditions in the step 2 to finally obtain the germinated Leymus chinensis seeds. The method disclosed by the invention is simple and easy, simple in operation, obvious in effect, and capable of increasing the germination rate of the shelled Leymus chinensis seeds to 75.42% after low-temperature (5 DEG C) treatment from 54.47% without the low-temperature (5 DEG C) treatment.

The present invention discloses a cold resistance correlated protein from leymus chinensis, an encoding gene thereof and an application thereof. The protein provided by the invention is (a) or (b): (a) a protein with an amino acid sequence as shown in a sequence 1 in a sequence list; (b) a protein obtained by substituting and/or deleting and/or adding one or more amino acid residues of the amino acid sequence as shown in the sequence 1 and related with plant cold resistance. The protein related with the plant cold resistance and the encoding gene Lc3919 of the protein provided by the invention are induced at a low temperature; a transgenic plant of the protein has relatively high cold resistance; according to the experiments, a wild type arabidopsis plant subjected to overnight cold stress at (-)8 DEG C only has a survival rate of 23.33% while the survival rate of the Lc3919 transgenic plant can be increased to 46.29% which is twice that of the survival rate of wild type arabidopsis. The Lc3919 gene can be used as a candidate gene of plant cold resistance genetic engineering for improving cold resistance of a plant.

The invention relates to the technical field of sandy land treatment and provides a method for sowing plant seeds in sand dunes to treat sandy land. The method includes: setting sand barriers at windgaps; applying base fertilizer; evenly mixing various seeds, and sowing 4-6kg of seeds into each mu of the sandy land, wherein the sown seed combination is optional one of the combination of Caraganamicrophylla, barley, Caragana korshinskii Kom. and wheatgrass, the combination of Hedysarum laeve Maxim, Artemisia desertorum, Caragana microphylla and wheatgrass, the combination of Artemisia halodendron, Achnatherum splendens, Medicago varia Martin. cv. Caoyuan No.3, Caragana microphylla, Calamagrostis epigejos, Artemisia frigida, Leymus secalinus, Leymus chinensis, Astragalus melilotoides Pall,Allium chrysanthum, Medicago sativa L. cv. Longmu 806, wild soybean, radix sileris, Elytrigia repens and Mengnong wheatgrass, and the like. By the method, plant coverage rate can reach 75-95% after the seeds sown into to the sand dunes grow into plants. The invention further provides a sandy land treatment method which comprises the method for sowing the plant seeds in the sand dunes to treat thesandy land and is capable of effectively treating the sandy land.

The invention discloses plant low-temperature resistance related protein, its encoded gene and application. The plant low-temperature resistance related protein provided in the invention is protein of (a) or (b) as the following that: (a) is protein composed of amino acid sequences as shown in sequence 1 of a sequence table; (b) is plant low-temperature resistance related protein that is derived from (a) and is obtained by substitution and/or deletion and/or adding of one or several amino acid residues in amino acid residue sequences as shown in sequence 1 of the sequence table. Experiments prove that, LcWRKY5 has tissue expression specificity, and under low-temperature induced expression, can participate in a response of Leymus chinensis to multiple kinds of adversity stress so as to improve stress resistance of plants. LcWRKY5 and its encoded gene are of great theoretical and practical significance for cultivating low-temperature resistance improved Leymus chinensis and other new plant varieties, and can be used for cultivating and identifying resistant plant varieties needed in agriculture and animal husbandry as well as ecological environment improvement, thus boasting high practical application value.

The invention discloses a method for constructing a mixed sowing grassland by reseeding alfalfa in a slightly degraded leymus chinensis meadow, and relates to a method for constructing an artificial grassland in a degraded leymus chinensis meadow. The invention aims to improve the community structure of the leymus chinensis meadow and improve the yield and quality of forage grass. The method comprises the following steps that 1, in early July, the slightly degraded leymus chinensis meadow is cut, stubbles of 3-5 cm are reserved, and light raking operation is conducted; 2, after light raking operation, alfalfa seeds are sown, the sowing rate is 10 kg/hectare, and after sowing, a compacting machine is used for slight compacting; 3, in the current year of alfalfa sowing, the grassland is forbidden to be cut, the grassland is cut twice every year from the next year, the cutting time is selected at the initial flowering stage of alfalfa each time, and after the first cutting, the grassland is fertilized. According to the method, the yield of high-quality forage grass can be increased by 1.5-2 tons/hectare per hectare, the price of the forage grass is 1000 yuan/ton, the income can be increased by at least 1500 yuan/hectare every year, and the economic benefit in the later period is very considerable. The invention belongs to the field of degraded grassland recovery.

The invention discloses a Leymus chinensis seed germinator, which is prepared from the following raw materials by weight: 24-26 parts of biogas residue, 10-12 parts of ears of sorghum, 5-6 parts of crude wood vinegar, 3-4 parts of micro silica, 2-3 parts of silica fume, 3-4 parts of polyethylene glycol, 55-56 parts of fresh Eupatorium adenophorum, 16-17 parts of fresh oyster, 1.3-1.5 parts of Pseudomonas aeruginosa microbial inoculum, 2.2-2.4 parts of high-temperature cellulolytic decomposer microbial inoculum, a proper amount of 6mol / L HCl solution and a proper amount of water. Aiming at low germination rate of Leymus chinensis seed, polyethylene glycol dissolve the organic matter layer such as a wax layer on the seed surface, so as to improve external material exchange ability of the seed with the outside, promote seed germination, significantly improve the germination rate of Leymus chinensis seeds and greatly promote large-scale cultivation of Leymus chinensis.