95 results about "Leymus" patented technology

The invention relates to a Chinese wildrye rhizosphere growth-promoting bacterium and application of the Chinese wildrye rhizosphere growth-promoting bacterium in growth promotion and drought resistance of Chinese wildrye, and belongs to the technical field of microorganisms, the strain is classified and named as Paraburkholderia graminis, the strain number is B24, and the preservation number is CGMCC No.31907. The strain provided by the invention is derived from the intergrassland soil of leymus chinensis in an excessive grazing grassland, has good growth promoting characteristics of producing IAA, producing ACC deaminase, fixing nitrogen, dissolving phosphorus and the like, and also can obviously enhance the drought resistance of the leymus chinensis. When the strain is inoculated to the rhizosphere of a leymus chinensis seedling, the growth of the leymus chinensis can be obviously promoted, and the overground and underground biomass of the leymus chinensis is increased; the activity of antioxidant enzymes is reduced and the hormone content in leymus chinensis leaves is changed, so that drought stress is relieved, the photosynthesis of leymus chinensis is enhanced, and the drought resistance of leymus chinensis is remarkably improved. Therefore, the Burkholderia parakholderia can be used for development of a microbial fertilizer for grassland plants and applied to a drought stress habitat, so that the drought resistance of the grassland plant leymus chinensis is improved, and grassland management is optimized.

The invention belongs to the technical field of whole-genome gene chips, and particularly relates to a leymus chinensis whole-genome 50K liquid chip and application thereof. According to the leymus chinensis whole genome 50K liquid phase chip, a whole genome re-sequencing technology is utilized, an SNP molecular marker combination of leymus chinensis is determined, and then the leymus chinensis whole genome 50K liquid phase chip is developed. The chip is combined with a high-density SNP probe, realizes high-throughput and high-precision genetic typing of leymus chinensis genomes, and particularly has wide application prospects in the field of molecular breeding.

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.

The invention discloses a Chinese wildrye hydroponic nutrient solution as well as a preparation method and application thereof, and belongs to the technical field of plant cultivation. Each liter of the nutrient solution comprises the following components: 80 to 120 mg of ammonium nitrate, 45 to 55 mg of sodium dihydrogen phosphate dihydrate, 80 to 90 mg of potassium sulfate, 110 to 115 mg of anhydrous calcium chloride, 400 to 410 mg of magnesium sulfate heptahydrate, 1.5 to 2.0 mg of manganese chloride tetrahydrate, 0.08 to 0.10 mg of ammonium molybdate tetrahydrate, 1.1 to 1.2 mg of boric acid, 0.02 to 0.05 mg of zinc sulfate heptahydrate, 0.02 to 0.05 mg of copper sulfate pentahydrate, 15 to 35 mg of ferrous sulfate heptahydrate and 20 to 50 mg of disodium ethylene diamine tetraacetate. The hydroponic nutrient solution provided by the invention meets the nutritional requirements of leymus chinensis, seedlings grow quickly and vigorously by utilizing the nutrient solution, the biomass and nutrient content of the overground part are remarkably increased, and the high-quality pasture characteristics of the leymus chinensis are ensured.

The invention discloses a growth-promoting bacterium and application thereof, and belongs to the technical field of microorganisms, the bacterial strain is classified and named as Phyllobacterium myrsinacearum, the bacterial strain number is B68, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.31906. The invention further discloses a preparation method of the growth-promoting bacterium. The strain provided by the invention is separated from intergrassland soil of overgrazing sample plots, and is obtained by artificial separation and purification. The strain has high yield of 3-indoleacetic acid, has strong dissolving capacity for insoluble phosphate, and has good growth promoting characteristics of nitrogen fixation, ACC deaminase production, IAA production and the like. The prepared strain fermentation liquor is inoculated to leymus chinensis seedlings, and the strain can be colonized among the leymus chinensis roots to adjust the bacterial community structure of the soil among the leymus chinensis roots; growth of leymus chinensis is obviously promoted, photosynthesis is enhanced, and chlorophyll content is increased; the content of hormones and nutrients in plant leaves and roots is obviously improved; the contents of rapidly available phosphorus, total carbon and organic matters in rhizosphere soil are obviously improved. Therefore, the ardisia japonica phyllobacterium can be used for subsequent development and related research of microbial fertilizers for grazing grassland.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The invention discloses application of a leymus chinensis U3 gene promoter proLcU3a, and belongs to the technical field of biology. The leymus chinensis U3 gene promoter proLcU3a with the nucleotide sequence shown as SEQ ID NO: 15 has the starting activity and can drive sgRNA transcription of a targeted leymus chinensis gene, so that a CRISPR-Cas9 gene editing system with the sgRNA transcription driven by the promoter proLcU3a can be constructed, editing of the leymus chinensis gene is realized, and the leymus chinensis U3 gene promoter proLcU3a can be applied to the technical fields of leymus chinensis genetic transformation, molecular breeding and the like.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.

The invention relates to the technical field of grassland ecological restoration, in particular to a root cutting device for a degraded leymus chinensis grassland and a restoration method. A suspension frame is arranged on a rack of the root cutting device, and the rack is constructed to walk on a sheep grass land along with movement of traction equipment. A punching assembly, a root cutting assembly and a fertilizing assembly are sequentially arranged on the rack in the walking direction. The punching assembly is configured to rotate along with walking of the rack and punch holes in the sheep grass land along the walking path of the rack, so that a plurality of punching belts are formed in the sheep grass land. The root cutting assembly is configured to rotationally cut in downwards from the surface of the leymus chinensis grassland through power transmission, loosen a soil hardening layer between the adjacent punching belts, cut off leymus chinensis roots in soil and construct a root cutting area between the punching belts along the walking path of the rack. The fertilizing assembly is used for fertilizing the root cutting area. According to the root cutting device for the degraded leymus chinensis grassland, the regeneration efficiency of leymus chinensis roots and the restoration quality of the grassland are improved.

The invention provides a shrub and grass matched wind prevention and sand fixation method under a photovoltaic panel of a mudshandack sand land. The method comprises the following steps: S1, planting herbaceous plants matched with small half shrubs in front of the photovoltaic panel; s2, adopting sand-barrier automatic sowing equipment to plant shrubs and weeds in and behind the photovoltaic panel; the herbaceous plants comprise artemisia desertorum, agriophyllum squarrosum, liquorice, wheatgrass, leymus chinensis and astragalus mongholicus in North China; the small half shrubs are caragana microphylla, caragana angustifolia and camellia hupehensis; the shrubs are red sand, caragana microphylla, caragana angustifolia, amygdalus pedunculata, alpinia hupehensis, salix babylonica, artemisia stelleriana, caragana microphylla and thyme; the weeds are agriophyllum squarrosum, agriophyllum squarrosum, pugionium Wind prevention and sand fixation engineering and photovoltaic industry land are ingeniously combined, adaptive vegetation is planted under the photovoltaic panel which is most prone to wind erosion and in the peripheral area, surface soil can be effectively fixed, sand dune mobility is reduced, a photovoltaic panel support foundation is protected against wind erosion damage, and the service life of a power station is prolonged.

The invention discloses a leymus chinensis accelerant and a method for repairing grassland by using the leymus chinensis accelerant, and belongs to the technical field of plant accelerant preparation. The method for repairing the grassland through the leymus chinensis accelerant comprises the following steps that P1, the underground part of leymus chinensis is cut up, and a pretreated leymus chinensis bud propagule is obtained; p2, spraying a leymus chinensis accelerant to the pretreated leymus chinensis bud propagules to obtain bud propagule segments; and P3, sowing the propagule section into the natural grassland by using a sowing machine, and repairing the grassland. By spraying the leymus chinensis accelerant, the vigor of leymus chinensis bud propagules can be effectively improved, the growth of leymus chinensis is promoted, and the biomass is increased, so that the restoration of grassland is realized.

The invention relates to the technical field of plant genetic engineering, and provides application of Leymus chinensis LcCBP60b gene in regulation and control of plant tiller number and biological yield. It is found for the first time that the LcCBP60b gene can improve the tiller number and the biological yield of leymus chinensis, and the LcCBP60b gene has important guiding significance on cultivation of high-yield and high-quality leymus chinensis varieties; the invention also clarifies the molecular mechanism of the LcCBP60b gene in regulation and control of the tiller number and the biological yield of Leymus chinensis, further analyzes the molecular module for regulating and controlling the tiller number and the biological yield of plants, can enrich the corresponding molecular mechanism, and provides a new breeding direction for breeding high-yield and high-quality varieties.

The invention relates to the technical field of feed mixing devices, in particular to a mixing device for Leymus chinensis single-cell protein feed processing, which comprises a stirrer, a stirring mechanism mounted on the stirrer, a dust removal mechanism, a spraying mechanism and a discharging mechanism, a premixing mechanism is mounted on the side surface of the stirrer, and a transmission mechanism is mounted on the dust removal mechanism; when forage is added into the stirrer and in the earlier stage of stirring, large feed dust can be raised, an axial flow fan is connected with an external power supply to suck out the feed dust on the stirrer, the feed dust is collected by a filter screen, clean air is discharged from the tail end of a connecting shell, and a first motor is started to drive a stirring shaft to rotate; the stirring shaft can drive the filter screen to rotate on the connecting shell after rotating, the filter screen can abut against the inner bottom of the connecting shell when rotating anticlockwise, the collected feed dust falls into the connecting hopper along with rotation of the filter screen, collection and treatment of the feed dust are facilitated, and air pollution and feed waste are avoided.

The invention relates to the technical field of plant tissue culture and plant transgenosis, in particular to a method for co-transformation of an LcSUCLA alpha-LcSUCLA beta-pEGFC binary expression vector and Leymus chinensis double genes. The method comprises the following steps: obtaining leymus chinensis tissue culture sterile calluses, transforming agrobacterium competent by a freeze thawing method, activating agrobacterium tumefaciens, infecting the leymus chinensis calluses and co-culturing, screening and culturing plant transformation materials, differentiating and screening a culture medium and culture conditions, and differentiating and screening a culture medium and culture conditions as well as a rooting culture medium and culture conditions. According to the invention, double-gene co-transformation of leymus chinensis is realized, and a leymus chinensis double-transgenic plant is obtained. The cotransformation of the LcSUCLA alpha and the LcSUCLA beta in the leymus chinensis can synthesize more succinic acid than the single transformation, so that the tolerance of the leymus chinensis under the stress of basic salt is further improved.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of the SNP molecular marker combination. The SNP molecular marker combination is composed of five SNP molecular markers, the SNP molecular markers in the group are located at the 80176348th site of a chromosome 6 of elymus dahuricus, the 490854164 site of a chromosome 9 of elymus dahuricus, the 291625249 site of a chromosome 11 of elymus dahuricus and the 64884340th site and the 65147058 site of a chromosome 12 of elymus dahuricus, base polymorphism CC / CG (GG), TT / TG, AA / TT, AA / GG and TT / CC exist at the sites, CC-TT-AA-AA-AA-TT is a saline-alkaline tolerant genotype, and CG / GG-TG-TT-GG-CC is a saline-alkaline sensitive The SNP molecular marker combination disclosed by the invention is used for detecting the saline-alkaline tolerance of the elymus dahuricus, is accurate and reliable, and effectively improves the identification efficiency of the germplasm and variety of the saline-alkaline tolerance elymus dahuricus.

The invention is applicable to the field of protoplast extraction application, and provides an enzymatic hydrolysate for Leymus chinensis protoplast extraction, which comprises the following components: 2.5-5% of cellulase R-10, 0.5-1% of macerozyme R-10, 0.7-1 M of mannitol, 5-9 mM of potassium chloride, 10-20 mM of MES buffer solution, 10-20 mM of calcium chloride, 0.05-0.2% of BSA, and the balance of ultrapure water. The invention also provides a preparation method and an application method of the enzymatic hydrolysate. In conclusion, according to the particularity of the leymus chinensis cell wall, an enzymatic hydrolysate formula is optimized, extraction conditions are adjusted, cell damage is effectively reduced, higher protoplast activity and quantity are obtained in leymus chinensis extraction, the extraction efficiency of the leymus chinensis protoplast is remarkably improved, and further conversion and gene expression research requirements are met.

The invention discloses a saline-alkali grassland management method based on ecological rotation and land sharing, which comprises the following steps: equally dividing a target land into a plurality of grid areas, collecting soil samples in different grid areas to measure the pH and total nitrogen content of soil, and dividing the soil into an ecological restoration area, a forage grass production area and an economic plant area according to the measured pH and total nitrogen content of the soil. Suaeda glauca and sesbania are planted in the ecological restoration area, alfalfa and leymus chinensis are planted in the forage grass production area, radix isatidis and purple perilla are planted in the economic plant area, and crop rotation planting is conducted on the three areas with two years as the cycle. According to the design, through complementation of plant communities, the fertilization requirement can be reduced, the soil remediation speed can be increased, grassland rotation management of multi-subject land sharing is achieved through ecological function complementation, the land utilization efficiency can be effectively improved, the grassland production-ecology-life three-generation functions are considered, and sustainable development of the saline-alkali grassland is promoted.

The invention provides a method for improving drought resistance of leymus chinensis under an extreme drought condition, and belongs to the technical field of drought-resistant cultivation. The invention provides an application of a salicylic acid solution in drought resistance of leymus chinensis, which proves that salicylic acid can participate in regulating stomatal movement of leymus chinensis, reduce water loss and improve the water utilization efficiency of plants; activation of an anti-oxidation system in plants can be promoted, and oxidative stress reaction caused by drought is relieved. In addition, the application of salicylic acid can also induce leymus chinensis to generate more stress response proteins and enhance the stability of cell walls, so that the survival rate of plants in a drought environment is increased. Therefore, the salicylic acid aqueous solution is applied to drought resistance of leymus chinensis, so that development of a new biotechnological means is facilitated, increasingly severe drought challenges are dealt with, and health and stability of a grassland ecosystem are protected.

The application discloses a method for promoting the absorption of methane in sheep grassland, and belongs to the field of environmental protection.The method is achieved by the following steps: applying nitrogen fertilizer to artificial sheep grassland in the growing season (June-September) to promote the absorption of methane in the grassland, so as to achieve the purpose of methane emission reduction.The nitrogen fertilizer added in the method is ammonium nitrate, the adding frequency is once a month, and the total dose is 100 kg N / hm 2 . Through the method, the total absorption amount of methane in the grassland in the growing season and the next spring freeze-thaw period is increased by more than 140% compared with the natural condition. The method is simple to operate and low in cost, and is helpful to the realization of the low-carbon target in the management process of artificial sheep grassland. In particular, in the next spring freeze-thaw period, the average value of the nitrogen application group is 7.1 mg m ‑2 , the average value of the nitrogen-free group is-5.0 mg m ‑2 , and the nitrogen application treatment increases the cumulative absorption amount of CH4 in the grassland soil in the spring freeze-thaw period by 240.4%.

This invention discloses a method for synergistically improving grassland plant diversity and productivity, relating to the field of degraded grassland ecological restoration technology. The method involves selecting degraded Leymus chinensis meadow steppe as the restoration target and delineating restoration plots. Within the operational window, targeted aeration and fertilization are carried out on the restoration plots to obtain improved restoration plots. These improved restoration plots are then continuously fenced off for at least two growing seasons for natural enclosure. This invention, through the synergistic effect of targeted aeration and precise fertilization, breaks through soil physical limitations and replenishes nutrient bottlenecks, achieving simultaneous improvement in plant diversity and productivity of degraded Leymus chinensis meadow steppe. It effectively optimizes the plant community structure and promotes the evolution of degraded communities into stable perennial herbaceous communities. Furthermore, this invention features highly standardized and mechanized operational parameters, high restoration efficiency, and is suitable for the large-scale restoration needs of degraded Leymus chinensis meadow steppe, balancing ecological benefits and production potential, and has broad application prospects.

The invention provides a plant growth regulator for improving the maturing rate of leymus chinensis and a spraying method of the plant growth regulator. The plant growth regulator for improving the maturing rate of Chinese wildrye comprises one or more of 2, 4-dichlorphenoxyacetic acid, indole-3-acetic acid, gibberellin and 6-benayl aminopurine. The concentration of the 2, 4-dichlorphenoxyacetic acid is 10 mg / L, the concentration of the indole-3-acetic acid is 2.8 mg / L, the concentration of the gibberellin is 2.5 mg / L, and the concentration of the 6-benayl aminopurine is 5 mg / L. The plant growth regulator for improving the maturing rate of leymus chinensis and the spraying method thereof have the advantages of being high in preparation efficiency and relatively simple in spraying method.

The application discloses application and a method of 1-Kestose in improving drought resistance of plants, and the drought resistance of plants is improved by using a 1-Kestose spraying agent, wherein the effective concentration of the leaf spraying agent is 0.1-10 muM, the optimal concentration of alfalfa is 10 muM, the concentration of Leymus chinensis, wheat and tall fescue is 1 muM, and the concentration of corn, cotton and ryegrass is 0.1 muM. The continuous leaf spraying is performed when the crops are two weeks old, and then drought stress treatment is performed. The drought resistance of crops can be significantly improved by externally applying trace 1-Kestose, and the method has the advantages of low concentration, simple operation, low cost, environmental friendliness and the like, and has a wide application prospect in drought-resistant agricultural production.

The invention discloses a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in a field, belongs to the field of crop genetic breeding, and particularly relates to a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in the field. In order to solve the problem of large character data error caused by initial non-uniformity and non-uniformity of transplanting damage of leymus chinensis tillering seedlings in existing field evaluation, the method comprises the following steps: 1, carrying out first homogenization treatment; 2, transplanting for the first time; 3, performing homogenization treatment for the second time; 4, transplanting for the second time; 5, observing a saline-alkaline tolerance phenotype; 6, evaluating saline-alkaline tolerance of the saline-alkaline tolerant plant germplasm. The core of the method is that two times of homogenization treatment and two times of transplanting are carried out on leymus chinensis tillering seedlings, the initial uniformity of test plants is greatly improved through the process, and systematic errors caused by the transplanting link are effectively controlled.

The invention provides a grass checkered sand barrier with a seed bank and a planting method and application of the grass checkered sand barrier, and belongs to the technical field of environmental governance. The planting method of the grass checkered sand-protecting barrier comprises the following steps: mixing and weaving the overground part of a drought-resistant and barren-resistant grass shrub perennial plant, a xerophyte cuttage plant branch and rice straw straws into a grass-protecting barrier, and planting the grass checkered sand-protecting barrier; the drought-tolerant and barren-tolerant grass shrub perennial plants are selected from at least two of ladywood, lespedeza floriflora, lespedeza tenuifolia and leymus chinensis at the upper part of a mowing field in a fruit maturation period; the xerophyte cutting plant is selected from one or more of yellow willow, salix psammophila and tamarix chinensis. In the grass checkered sand barrier, the rice straw plays a role in protection, the cutting branches have a cutting survival function, seeds carried by perennial plants can germinate and grow plants in good time under the shielding of the grass checkered sand barrier, vegetation in sand or desert areas can be recovered, the vegetation coverage and density are improved, and the survival rate of the perennial plants is increased. The vegetation coverage can be improved by more than 30% compared with that of an exposed plot after planting for 2 years.

The method for saline-alkali land remediation based on simultaneous activation of Leymus chinensis rhizome and sexual complementary sowing in different layers belongs to the technical field of ecological remediation and sustainable utilization of grassland. The method solves the technical problem that there is currently a lack of a collaborative remediation technology that can combine low disturbance, activation of the original Leymus chinensis vegetative propagation potential, and efficient introduction of sexual reproduction. The method comprises the following steps: S1, determining a saline-alkali region that needs to be remediated; S2, determining a time window for remediation operation; S3, implementing narrow-width low-disturbance deep ploughing, and leaving a width between adjacent deep ploughing strips that is greater than the width of the deep ploughing narrow strips; S4, implementing in-situ collaborative seeding and directional root induction, and synchronously implementing precision seeding, positioning fertilization and habitat directional induction in the narrow strips where deep ploughing is completed by using a mechanical linkage device; and S5, field management.

The present invention relates to a plant growth-promoting rhizobacterium of Leymus chinensis and its application in promoting the growth and drought resistance of Leymus chinensis, belonging to the field of microbial technology. The strain is classified and named as Paraburkholderia graminis , with the strain number B24 and the preservation number CGMCC No. 31907. The strain of the present invention is derived from the rhizosphere soil of Leymus chinensis in overgrazed grasslands. This strain not only has good plant growth-promoting characteristics such as IAA production, ACC deaminase production, nitrogen fixation, and phosphorus solubilization, but also can significantly enhance the drought resistance of Leymus chinensis. Inoculating this bacterium into the rhizosphere of Leymus chinensis seedlings can significantly promote the growth of Leymus chinensis, increase the aboveground and underground biomass of Leymus chinensis; reduce the antioxidant enzyme activity and change the hormone content in the leaves of Leymus chinensis, thereby alleviating drought stress, enhancing the photosynthesis of Leymus chinensis, and thus significantly improving the drought resistance of Leymus chinensis. Therefore, the Burkholderia parakniphia of the present invention can be used for the development of microbial fertilizers for grassland plants and applied in arid stress habitats, so as to improve the drought resistance of the grassland plant Leymus chinensis and optimize grassland management.

The invention relates to the technical field of image processing, in particular to a leymus chinensis seed setting rate obtaining method, electronic equipment and a storage medium, and the method comprises the following steps: obtaining a leymus chinensis spike X-ray graph corresponding to a leymus chinensis sample, and carrying out pixel segmentation processing on the leymus chinensis spike X-ray graph based on a target pixel segmentation threshold to obtain a leymus chinensis seed setting rate; the method comprises the following steps of: obtaining a binary image corresponding to a Leymus chinensis spike X-ray graph, determining a plurality of Leymus chinensis spike regions and a plurality of Leymus chinensis granular regions from the binary image, performing image pixel iterative segmentation on the Leymus chinensis spike regions and the Leymus chinensis granular regions to obtain a plurality of second seed regions, and obtaining a plurality of target floret regions according to the binary image, the proportion of the number of the second seed areas to the number of the target floret areas is determined as the single plant setting rate corresponding to the Leymus chinensis samples, and then the Leymus chinensis seed setting rate is obtained.According to the method, the Leymus chinensis seeds and floret can be automatically and rapidly recognized in a high-throughput mode, and then the Leymus chinensis seed setting rate is accurately and rapidly obtained.

The invention provides application of a protein LcSAT1 and a related biological material of the protein LcSAT1. The amino acid sequence of the LcSAT1 protein is as shown in SEQ ID No: 2. Wild leymus chinensis is used as a receptor material, and an LcSAT1 overexpression plant is obtained. Compared with a wild type, the stress resistance of an LcSAT1 overexpressed plant is remarkably improved. The LcSAT1 and the coding gene thereof have important theoretical and practical significance for cultivating leymus chinensis and other new plant varieties with improved stress resistance, can be applied to cultivation and identification of resistant plant varieties required by agriculture and animal husbandry and ecological environment management, and have relatively high practical application value. The method has a wide application prospect in the field of agriculture.

The invention discloses a leymus chinensis planting integrated operation cooperation system applying intelligent control, and relates to the technical field of intelligent agriculture, and the system comprises a cloud server which is in communication connection with the following modules: a perception decision cooperation module which is used for comprehensively collecting soil parameters to construct a soil attribute spatial distribution map, and sending the soil attribute spatial distribution map to the cloud server; and a rotary tillage strategy is optimized by adopting cloud collaboration and a fruit fly algorithm, and the rotary tillage depth is determined so as to carry out adaptive regulation and control on the rotary tillage unit. A high-precision soil attribute spatial distribution diagram is constructed by collecting soil parameters, an optimal pressing depth combination of each rotary tillage unit is quickly calculated by using a fruit fly algorithm, and an instruction is issued to dynamically adjust a hydraulic execution mechanism, so that the limitation of a traditional mechanical fixed operation mode is broken through; an optimal soil physical environment is created for healthy growth of leymus chinensis roots, and the problem that the roots are difficult to penetrate through hard soil layers due to high spatial heterogeneity of soil hardness is fundamentally solved.

The invention provides a method for recovering grassland between photovoltaic panels, and belongs to the technical field of grassland recovery. The recovery method comprises the steps that during soil preparation and deep scarification, crop waste is thrown, and an organic interlayer is constructed; meanwhile, base fertilizer is applied during soil preparation and deep scarification; the deep scarification depth ranges from 30 cm to 40 cm; before sowing, microbial fertilizer treatment is carried out, pasture mixed seeds are sown, and the pasture mixed seeds are northeast leymus chinensis, puccinellia koreana and elymus dahuricus. According to the method, through the synergistic effect of the northeast Chinese wildrye, the puccinellia koreana and the elymus dahuricus and the methods of organic interlayer salt resistance, fertilization, microbial fertilizer treatment and the like, the vegetation coverage, community density and overground biomass of the grassland between the photovoltaic panels are remarkably improved, the alkali spot area proportion of the grassland between the photovoltaic panels and the pH value of 0-20 cm surface soil are reduced, and compared with the current sowing year, the method has the advantages that the cost is reduced, and the yield is increased. The emergence rate of the pasture in the second year of sowing, the fresh weight of the overground plants and the dry weight of the overground plants are remarkably increased, and the recovery efficiency and ecological benefits of the grassland between the photovoltaic panels are improved.