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35 results about "Leymus" patented technology

Leymus is a genus of plants in the grass family Poaceae (Gramineae). It is widespread across Europe, Asia, and the Americas.

Rapid restoration method for degraded grassland based on microbial agent

The invention discloses a rapid restoration method for degraded grassland based on a microbial agent. The microbial agent is formed by compounding 15-30 parts of a nitrogen-fixing microbial agent, 10-20 parts of a phosphate-solubilizing microbial agent, 8-15 parts of a plant growth-promoting microbial agent, 25-40 parts of a humic acid carrier, 3-8 parts of an embedding agent sodium alginate and 5-12 parts of a nutritional agent molasses. Wherein the nitrogen-fixing bacteria are azotobacter chroococcum and alfalfa rhizobium (the viable count is greater than or equal to 5 * 10 < 9 > CFU / g), the phosphate solubilizing bacteria are bacillus megatherium (the viable count is greater than or equal to 1 * 10 < 10 > CFU / g), and the growth-promoting bacteria are bacillus subtilis and pseudomonas fluorescens (the viable count is greater than or equal to 2 * 10 < 10 > CFU / g). The microbial inoculum is subjected to activation, carrier adsorption, sodium alginate embedding granulation and CaCl2 cross-linking processes to be prepared into slow-release particles of 1-3 mm. 200-250 kg of organic fertilizer is applied to each hectare in a mild area, 300-400 kg of organic fertilizer is applied to each hectare in a moderate and severe area, and leymus chinensis and wheatgrass are sown in a mixed mode. After 90 days of application, the vegetation coverage is improved by 40% or above, the soil organic matter is increased by 25-40%, the microbial diversity index is larger than or equal to 3.5, and the comprehensive remediation cost is reduced by 53%.
Owner:BAIHULING FOREST FARM OF GUANDI MOUNTAIN STATE FORESTRY ADMINISTRATION OF SHANXI PROVINCE

Application of LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis

The invention belongs to the technical field of plant genetic engineering, and discloses application of an LcCBP60b-LcALY2 molecular module in regulation and control of growth and development and maturing rate of leymus chinensis. The invention discovers that the calmodulin binding protein coding gene LcCBP60b negatively regulates the growth and development and the maturing rate of the leymus chinensis for the first time, and a yeast double-impurity experiment discovers that the LcCBP60b interacts with the LcALY2 protein to regulate the related characters of the maturing rate of the leymus chinensis. The LcCBP60b negatively regulates the activity of the LcALY2 protein and inhibits the regulation effect of the LcALY2 coded THO protein complex 4B in the flowering and ear forming process of leymus chinensis. The LcCBP60b-LcALY2 molecular module verified by the invention provides a new perspective for deeply understanding a Leymus chinensis fruiting regulation mechanism, and provides a direct molecular target for directionally creating new varieties of high-fruiting-rate pasture and cereal crops.
Owner:CHINA AGRI UNIV

Method suitable for planting, carbon sequestration and sand stabilization under photovoltaic panel in muddy Dacromet sand land

The invention provides a shrub and grass matched wind prevention and sand fixation method under a photovoltaic panel of a mudshandack sand land. The method comprises the following steps: S1, planting herbaceous plants matched with small half shrubs in front of the photovoltaic panel; s2, adopting sand-barrier automatic sowing equipment to plant shrubs and weeds in and behind the photovoltaic panel; the herbaceous plants comprise artemisia desertorum, agriophyllum squarrosum, liquorice, wheatgrass, leymus chinensis and astragalus mongholicus in North China; the small half shrubs are caragana microphylla, caragana angustifolia and camellia hupehensis; the shrubs are red sand, caragana microphylla, caragana angustifolia, amygdalus pedunculata, alpinia hupehensis, salix babylonica, artemisia stelleriana, caragana microphylla and thyme; the weeds are agriophyllum squarrosum, agriophyllum squarrosum, pugionium Wind prevention and sand fixation engineering and photovoltaic industry land are ingeniously combined, adaptive vegetation is planted under the photovoltaic panel which is most prone to wind erosion and in the peripheral area, surface soil can be effectively fixed, sand dune mobility is reduced, a photovoltaic panel support foundation is protected against wind erosion damage, and the service life of a power station is prolonged.
Owner:MENGCAO ECOLOGICAL ENVIRONMENT (GRP) CO LTD

SNP molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of SNP molecular marker combination

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination related to saline-alkaline tolerance of elymus dahuricus and application of the SNP molecular marker combination. The SNP molecular marker combination is composed of five SNP molecular markers, the SNP molecular markers in the group are located at the 80176348th site of a chromosome 6 of elymus dahuricus, the 490854164 site of a chromosome 9 of elymus dahuricus, the 291625249 site of a chromosome 11 of elymus dahuricus and the 64884340th site and the 65147058 site of a chromosome 12 of elymus dahuricus, base polymorphism CC / CG (GG), TT / TG, AA / TT, AA / GG and TT / CC exist at the sites, CC-TT-AA-AA-AA-TT is a saline-alkaline tolerant genotype, and CG / GG-TG-TT-GG-CC is a saline-alkaline sensitive The SNP molecular marker combination disclosed by the invention is used for detecting the saline-alkaline tolerance of the elymus dahuricus, is accurate and reliable, and effectively improves the identification efficiency of the germplasm and variety of the saline-alkaline tolerance elymus dahuricus.
Owner:INNER MONGOLIA AGRICULTURAL UNIVERSITY

A method for promoting methane uptake in a leymus chinensis grassland

ActiveCN118985248BFertilising methodsGrowing seasonLeymus
The application discloses a method for promoting the absorption of methane in sheep grassland, and belongs to the field of environmental protection.The method is achieved by the following steps: applying nitrogen fertilizer to artificial sheep grassland in the growing season (June-September) to promote the absorption of methane in the grassland, so as to achieve the purpose of methane emission reduction.The nitrogen fertilizer added in the method is ammonium nitrate, the adding frequency is once a month, and the total dose is 100 kg N / hm 2 . Through the method, the total absorption amount of methane in the grassland in the growing season and the next spring freeze-thaw period is increased by more than 140% compared with the natural condition. The method is simple to operate and low in cost, and is helpful to the realization of the low-carbon target in the management process of artificial sheep grassland. In particular, in the next spring freeze-thaw period, the average value of the nitrogen application group is 7.1 mg m ‑2 , the average value of the nitrogen-free group is-5.0 mg m ‑2 , and the nitrogen application treatment increases the cumulative absorption amount of CH4 in the grassland soil in the spring freeze-thaw period by 240.4%.
Owner:NORTHEAST NORMAL UNIVERSITY

A method for synergistically improving grassland plant diversity and productivity

This invention discloses a method for synergistically improving grassland plant diversity and productivity, relating to the field of degraded grassland ecological restoration technology. The method involves selecting degraded Leymus chinensis meadow steppe as the restoration target and delineating restoration plots. Within the operational window, targeted aeration and fertilization are carried out on the restoration plots to obtain improved restoration plots. These improved restoration plots are then continuously fenced off for at least two growing seasons for natural enclosure. This invention, through the synergistic effect of targeted aeration and precise fertilization, breaks through soil physical limitations and replenishes nutrient bottlenecks, achieving simultaneous improvement in plant diversity and productivity of degraded Leymus chinensis meadow steppe. It effectively optimizes the plant community structure and promotes the evolution of degraded communities into stable perennial herbaceous communities. Furthermore, this invention features highly standardized and mechanized operational parameters, high restoration efficiency, and is suitable for the large-scale restoration needs of degraded Leymus chinensis meadow steppe, balancing ecological benefits and production potential, and has broad application prospects.
Owner:INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI

1 -kestose for use in improving drought resistance in plants and methods

PendingCN122139753ABiocidePlant growth regulatorsBiotechnologyLolium perenne
The application discloses application and a method of 1-Kestose in improving drought resistance of plants, and the drought resistance of plants is improved by using a 1-Kestose spraying agent, wherein the effective concentration of the leaf spraying agent is 0.1-10 muM, the optimal concentration of alfalfa is 10 muM, the concentration of Leymus chinensis, wheat and tall fescue is 1 muM, and the concentration of corn, cotton and ryegrass is 0.1 muM. The continuous leaf spraying is performed when the crops are two weeks old, and then drought stress treatment is performed. The drought resistance of crops can be significantly improved by externally applying trace 1-Kestose, and the method has the advantages of low concentration, simple operation, low cost, environmental friendliness and the like, and has a wide application prospect in drought-resistant agricultural production.
Owner:NANJING AGRICULTURAL UNIVERSITY

Method for evaluating saline-alkaline resistance of different Leymus chinensis germplasm resources in field

The invention discloses a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in a field, belongs to the field of crop genetic breeding, and particularly relates to a method for evaluating saline-alkaline tolerance of different Leymus chinensis germplasm resources in the field. In order to solve the problem of large character data error caused by initial non-uniformity and non-uniformity of transplanting damage of leymus chinensis tillering seedlings in existing field evaluation, the method comprises the following steps: 1, carrying out first homogenization treatment; 2, transplanting for the first time; 3, performing homogenization treatment for the second time; 4, transplanting for the second time; 5, observing a saline-alkaline tolerance phenotype; 6, evaluating saline-alkaline tolerance of the saline-alkaline tolerant plant germplasm. The core of the method is that two times of homogenization treatment and two times of transplanting are carried out on leymus chinensis tillering seedlings, the initial uniformity of test plants is greatly improved through the process, and systematic errors caused by the transplanting link are effectively controlled.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Saline-alkali land remediation method based on simultaneous activation of leymus chinensis rhizome and sexual complementary sowing in different layers

The method for saline-alkali land remediation based on simultaneous activation of Leymus chinensis rhizome and sexual complementary sowing in different layers belongs to the technical field of ecological remediation and sustainable utilization of grassland. The method solves the technical problem that there is currently a lack of a collaborative remediation technology that can combine low disturbance, activation of the original Leymus chinensis vegetative propagation potential, and efficient introduction of sexual reproduction. The method comprises the following steps: S1, determining a saline-alkali region that needs to be remediated; S2, determining a time window for remediation operation; S3, implementing narrow-width low-disturbance deep ploughing, and leaving a width between adjacent deep ploughing strips that is greater than the width of the deep ploughing narrow strips; S4, implementing in-situ collaborative seeding and directional root induction, and synchronously implementing precision seeding, positioning fertilization and habitat directional induction in the narrow strips where deep ploughing is completed by using a mechanical linkage device; and S5, field management.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Leymus chinensis planting integrated operation cooperation system applying intelligent control

The invention discloses a leymus chinensis planting integrated operation cooperation system applying intelligent control, and relates to the technical field of intelligent agriculture, and the system comprises a cloud server which is in communication connection with the following modules: a perception decision cooperation module which is used for comprehensively collecting soil parameters to construct a soil attribute spatial distribution map, and sending the soil attribute spatial distribution map to the cloud server; and a rotary tillage strategy is optimized by adopting cloud collaboration and a fruit fly algorithm, and the rotary tillage depth is determined so as to carry out adaptive regulation and control on the rotary tillage unit. A high-precision soil attribute spatial distribution diagram is constructed by collecting soil parameters, an optimal pressing depth combination of each rotary tillage unit is quickly calculated by using a fruit fly algorithm, and an instruction is issued to dynamically adjust a hydraulic execution mechanism, so that the limitation of a traditional mechanical fixed operation mode is broken through; an optimal soil physical environment is created for healthy growth of leymus chinensis roots, and the problem that the roots are difficult to penetrate through hard soil layers due to high spatial heterogeneity of soil hardness is fundamentally solved.
Owner:BAICHENG ANIMAL HUSBANDRY SCI RES INST +1

Saline-alkali soil remediation method based on synchronous different-layer activation and sexual reseeding of leymus chinensis rhizomes

The invention discloses a saline-alkali soil remediation method based on leymus chinensis rhizome activation and sexual reseeding synchronous different layers. Belongs to the technical field of ecological restoration and grassland sustainable utilization. The technical problem that at present, a synergistic remediation technology which can be combined with low disturbance, activate the asexual propagation potential of the native leymus chinensis and efficiently introduce sexual propagation is lacked is solved. The method comprises the following steps: S1, determining a saline-alkali land area needing to be repaired; s2, determining a time window for repairing operation; s3, narrow low-interference deep scarification is implemented, and the reserved width between every two adjacent deep scarification strips is larger than the width of a deep scarification narrow strip; s4, implementing in-situ collaborative reseeding and directional root guiding of the seeds, and synchronously implementing precision seeding, positioned fertilization and habitat directional induction by using a mechanical linkage device in a narrow strip after deep scarification; s5, field management.
Owner:NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S

Application of LcCBP60b-LcNAC2-LcALY2 molecular module in regulating and controlling related traits of maturing rate of leymus chinensis

The invention belongs to the technical field of biology, and discloses an application of an LcCBP60b-LcNAC2-LcALY2 molecular module in regulating and controlling related traits of the maturing rate of leymus chinensis. The invention provides and verifies that the molecular module LcCBP60b-LcNAC2 regulates the transcription of LcALY2, and further regulates the formation of the setting rate character of Leymus chinensis. Compared with a wild type, the LcCBP60b silent transgenic plant has the advantages that the maturing rate is obviously increased, the ear length and the spikelet number are obviously increased, in the LcCBP60b silent transgenic plant, the transcription level of the LcNAC2 gene is reduced, and the inhibition effect of an LcNAC2 transcription factor on LcALY2 is reduced. The regulation and control change is helpful to stimulate the transmission of flowering and seed development related signals in the leymus chinensis, so that the maturing rate of the leymus chinensis is improved. The invention lays a technical foundation for improving the maturing rate of leymus chinensis and cereal crops through molecular design breeding.
Owner:CHINA AGRI UNIV

Use of lc sfc6 protein and related biological materials

The application provides application of LcSFC6 protein and related biological materials thereof. The amino acid sequence of the LcSFC6 protein is shown as SEQ ID No: 2. The application takes wild Leymus chinensis as a receptor material to obtain LcSFC6 The overexpression plant of the gene. Compared with the wild type, LcSFC6 The stress resistance of the overexpression plant is significantly improved. The LcSFC6 and the coding gene thereof have important theoretical and practical significance for cultivating new varieties of Leymus chinensis and other plants with improved stress resistance, and can be applied to cultivation and identification of plant varieties with resistance required by agriculture, animal husbandry and ecological environment management, and have high practical application value. The application has wide application prospect in the field of agriculture.
Owner:INST OF BOTANY CHINESE ACAD OF SCI

Methods and apparatus for determining nitrogen fertilizer application rate for Leymus chinensis forage grasses

This application provides a method and apparatus for determining the nitrogen fertilizer application rate for *Leymus chinensis* forage. The method includes: collecting growth data and biological samples of target *Leymus chinensis* forage within a target area; determining the aboveground dry matter, belowground dry matter, and nitrogen concentration data of the target *Leymus chinensis* forage using the biological samples; constructing a first critical nitrogen dilution curve based on the aboveground dry matter and nitrogen concentration data; and determining the nitrogen fertilizer application rate for the target area based on the belowground dry matter, the first critical nitrogen dilution curve, and the growth data. This method enables precise application of nitrogen fertilizer during the production of *Leymus chinensis* forage, ensuring yield and root growth while reducing fertilization costs and environmental pollution.
Owner:SOUTHWEST UNIVERSITY FOR NATIONALITIES

Method for obtaining selfing fruiting new variety of leymus chinensis and molecular marker thereof

The invention belongs to the technical field of molecular marker breeding, and particularly relates to a method for obtaining a new selfing and fruiting variety of leymus chinensis, a molecular marker LcDUF247II-S3 and application of the molecular marker LcDUF247II-S3.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1

Desulfurized gypsum and fly ash experimental device and method for rebuilding leymus chinensis vegetation in mining area

The invention discloses a desulfurized gypsum and fly ash experimental device and method for mining area leymus chinensis vegetation reconstruction, and relates to the technical field of ecological restoration, the desulfurized gypsum and fly ash experimental device comprises a mounting cover, a mixing box, a stirring mechanism, a flapping mechanism, a conveying belt, a placing box, a culture basin, an air freezing and thawing testing machine, a detection machine, a data processing end and a cleaning machine, the rear end of the top in the mounting cover is fixedly connected with a mixing box, a stirring mechanism is arranged, through rotation and movement of two sets of weak direct-current electric field mechanisms and work of an ultrasonic atomization spray head, material caking is broken, mixing dead corners are eliminated, the uniformity of matrixes with different proportions is accurately regulated and controlled, meanwhile, materials penetrate, dead-corner-free infiltration is achieved, and the mixing efficiency is improved. The interface combination of the mineral substances, the organic matters and the biological bacterial liquid is enhanced, the matrix stability and the nutrient effectiveness are improved, and a uniform and high-activity mixed system is provided for the construction of the ternary composite artificial soil.
Owner:TONGLIAO ENVIRONMENTAL PROTECTION INVESTMENT CO LTD

Preparation method and application of multi-element synergistic compost material for repairing heavily degraded grassland

The invention discloses a preparation method and application of a multi-element synergistic compost material for repairing heavily-degraded grassland, and relates to the technical field of ecological restoration of the heavily-degraded grassland. According to the preparation method and application of the multi-element synergistic compost material for repairing the severely degraded grassland, the compost period is shortened to about 35 days by optimizing the compost raw material ratio, the fermentation process and the turning strategy, and compared with the effective period of 3-5 years in the prior art, the early-stage effect development speed of repairing the degraded grassland is greatly increased, and the cost is reduced. After the compost material is applied, the physical and chemical environment of soil can be quickly improved, and suitable conditions are provided for vegetation growth. Meanwhile, a multi-element synergistic composting system and a scientific application method form resultant force, so that the soil structure of the severely degraded grassland is effectively improved, the soil bulk density is remarkably reduced, the pH value is close to neutral, the salt content is greatly reduced, the comprehensive quality index of the soil is remarkably improved compared with a control group, and the survival rate and the growth index of leymus chinensis are further remarkably improved.
Owner:NORTHEAST NORMAL UNIVERSITY

Biological product for regulating tiller number by espti15 gene expression

This invention relates to the field of biotechnology, specifically to the application of bioproducts expressing the EsPTI15 gene to regulate tiller number. The nucleotide sequence of the EsPTI15 gene is shown in SEQ ID NO:1. This invention is the first to discover that, compared to wild-type *Leymus chinensis*, EsPTI1… 5 Overexpression of the EsPTI15 gene significantly increased the number of tillers in *Triticum aestivum*, confirming that overexpression of the EsPTI15 gene positively regulates tillering development. This finding not only enriches the molecular mechanism theory of plant tillering regulation but also demonstrates the potential of the EsPTI15 gene to increase the number of tillers, paving the way for subsequent gene manipulation to introduce EsPTI1. 5 The research and development of technologies that increase the number of tillers in forage plants and thus improve biomass has provided key theoretical support and genetic resources.
Owner:INSTITUTE OF ANIMAL SCIENCES OF CHINESE ACADEMY OF AGRICULTURAL SCIENCES

A method for underground drip irrigation of puccinellia distans in soda saline soil

The application belongs to the technical field of planting and soil improvement method of Leymus chinensis, and relates to a method for underground drip irrigation of Leymus chinensis in soda saline-alkali soil, and specifically comprises the following steps: step one, drip irrigation of humic acid-citric acid complex and potassium dihydrogen phosphate solution in the early stage; drip irrigation of gypsum-sulfur activated suspension and potassium silicate solution in the middle stage; drip irrigation of bacteria-algae complex and zinc sulfate-ammonium molybdate mixed solution in the late stage; drip irrigation of humic-like polymer in the intermittent stage; and after each time of drip irrigation, the dripper is flushed with a sugar lipid solution; through the time-sequencing targeted drip irrigation system, the method realizes the trinity of "soil improvement, growth promotion and soil nurturing" of Leymus chinensis planting in soda saline-alkali soil, solves three technical problems of salt recirculation, loss of biological activity and system maintenance difficulty, and provides a closed-loop solution for ecological restoration of saline-alkali soil.
Owner:INST OF WATER RESOURCES FOR PASTERAL AREA MINIST OF WATER RESOURCES P R C

Methods for obtaining new self-pollinating varieties of Leymus chinensis and the self-pollinating gene of Leymus chinensis

This invention belongs to the field of molecular marker breeding technology, specifically relating to a method for obtaining new self-fertile varieties of Leymus chinensis and the self-fertile gene and its application.
Owner:INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI +1

Leymus chinensis seed low-loss high-cleanliness separating screen

The utility model discloses a leymus chinensis seed low-loss high-net separating screen, which comprises a separating screen body, a top screen, a middle screen and a bottom screen, the top screen, the middle screen and the bottom screen are sequentially mounted on the separating screen body from top to bottom, the top screen comprises a first screen and a second screen which are arranged next to each other front and back, the first screen is a wave-shaped screen, the second screen comprises a front screen and a rear screen, and the front screen is a wave-shaped screen. The front screen comprises a plurality of sawtooth-shaped screen pieces arranged in the cleaning direction at intervals and a plurality of adjusting long strip pieces which are perpendicular to the cleaning direction and arranged in parallel at intervals, and the angle of the adjusting long strip pieces can be adjusted. The rear screen is a woven screen. The middle-layer sieve comprises a small-section wave sieve and a rear woven sieve which are arranged next to each other front and back; and the bottom-layer sieve is a fish scale sieve. The separating screen device is installed on the combined harvester for the leymus chinensis seeds, and through structural innovation of the separating screen device, the harvesting loss of the leymus chinensis seeds can be effectively reduced, the harvesting cleanliness of the leymus chinensis seeds is improved, the content of impurities in a leymus chinensis seed mixture is reduced, and the labor intensity of follow-up treatment of the leymus chinensis seeds is reduced.
Owner:INSTITUTE OF GRASSLAND RESEARCH OF CAAS

Application of LcCBP60b gene in regulating tiller number and biomass of Leymus chinensis

This invention relates to the field of plant genetic engineering technology, and provides sheepgrass. LcCBP60b The application of genes in regulating plant tiller number and biomass yield. This invention is the first to discover... LcCBP60b Genes can increase the number of tillers and biomass of Leymus chinensis, which is of great guiding significance for breeding high-yield and high-quality Leymus chinensis varieties; this invention also clarifies that LcCBP60b Investigating the molecular mechanisms by which genes regulate tiller number and biomass of Leymus chinensis, and further elucidating the molecular modules that regulate plant tiller number and biomass, can enrich the corresponding molecular mechanisms and provide new breeding directions for selecting high-yield and high-quality varieties.
Owner:CHINA AGRI UNIV

Paddy field post-emergence weeding composition and application thereof

PendingCN121264478ABiocideAnimal repellantsPropargiteOrganic chemistry
The invention belongs to the field of agricultural chemical application, and particularly relates to a synergistic weeding composition and application of the synergistic weeding composition in prevention and removal of moleplant seeds, the weeding composition is composed of three active ingredients of dichloro-clomazone, cyhalofop-butyl and propanil, the weight ratio of the dichloro-clomazone to the cyhalofop-butyl to the propanil is 1: (2-6): (5-15), and the preferable weight ratio is 1: 4: 10. The weeding composition is prepared into missible oil through dosage form processing, is diluted with water into a milk white homogeneous state, is used for controlling annual weeds in paddy fields through stem and leaf spraying, and has an excellent control effect on moleplant seeds.
Owner:ZHEJIANG TIANFENG BIOLOGICAL SCI

A type of rough-spike wheatgrass 1H t S chromosome-specific KASP molecular markers and their applications

PendingCN122326797ANucleotideElymus
This invention discloses a type of Leymus chinensis 1H t This paper discusses S-chromosome-specific KASP molecular markers and their applications, relating to the fields of biotechnology and crop genetics and breeding. The KASP molecular marker is KASP-1H. t The primers for this marker include an upstream primer for the *Elymus styrax* allele, an upstream primer for the wheat allele, and a shared downstream primer, with nucleotide sequences shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively. This marker possesses advantages such as good genetic stability, high specificity, high resolution, and high detection efficiency. It enables high-throughput, automated genotyping without gel electrophoresis, and can accurately detect and track *Elymus styrax* 1H in the complex genetic background of wheat. t The S chromosome. This marker can be widely used in the identification of wheat-Leymus chinensis distant hybrids, chromosome engineering breeding, and molecular marker-assisted selection breeding, providing important technical support for the efficient transfer and utilization of superior genes in Leymus chinensis.
Owner:CROP RES INST SHANDONG ACAD OF AGRI SCI

Enterobacter cloacae strain and application thereof

The invention is applicable to the technical field of microorganisms, and provides an enterobacter cloacae strain and application thereof, the strain is named as enterobacter cloacae DJ, and is preserved in the China Center for Type Culture Collection on July 2, 2025, and the preservation number is CCTCC NO: M20251510. The enterobacter cloacae DJ is separated from rhizosphere soil of Leymus chinensis Tzvel in the western grassland region of Jilin Province, the enterobacter cloacae DJ has a growth promoting effect under the saline-alkaline condition, the germination rate of crop seeds can be obviously increased after the enterobacter cloacae DJ is treated with a bacterial liquid under the saline-alkaline condition, the plant resistance under the saline-alkaline condition can be improved, the utilization rate of saline-alkaline land can be increased, and the application prospect is wide. The method has a wide application prospect in agricultural production.
Owner:CHANGCHUN NORMAL UNIV

Intelligent counting method based on leymus chinensis tillering number

The invention relates to the technical field of agricultural information, and discloses an intelligent counting method based on leymus chinensis tillering quantity. According to the method, band fusion is carried out on a leymus chinensis canopy multispectral image to enhance features, then effective tillering clusters are detected and screened out, and sub-pixel-level edge refinement is carried out on the effective tillering clusters. Constructing a tillering connection graph based on the spatial topological relation of the tillering units, and applying a graph segmentation algorithm to remove tillering adhesion and divide overlapping region affiliation; afterwards, a framework of the independent tillering unit is extracted, branch points are analyzed, merging and false removing are carried out according to consistency constraints of space distribution of new tillering points and the growth direction of a main stem, and a final tillering node is confirmed. And finally, counting the number of nodes, performing time sequence verification and correction in combination with historical data, and associatively storing a result. The leymus chinensis tillering automatic counting device realizes automatic and high-precision counting of leymus chinensis tillering.
Owner:INSTITUTE OF GRASSLAND RESEARCH OF CAAS

Saline-alkali land leymus chinensis root planting device and method

The invention relates to the technical field of planting, and discloses a saline-alkali land leymus chinensis root planting device and method.The saline-alkali land leymus chinensis root planting device comprises a walking frame, a soil preparation frame is evenly arranged at the bottom of one end of the walking frame, a furrow opener is arranged at one end of the soil preparation frame, a soil covering plate is arranged at the other end of the soil preparation frame, and a conveying belt used for conveying leymus chinensis roots is arranged on one side of the upper end of the walking frame; transplanting cylinders in one-to-one correspondence with the soil preparation frames are uniformly arranged at one end of the walking frame, and a feeding opening corresponding to the conveying belt is formed in one side of the upper end of each transplanting cylinder. According to the device, through the synergistic effect of the conveying belt, the protective cylinder, the soil shifting plate and the supporting semi-rings, flexible conveying and precise guiding of rhizome soil balls are achieved, it is guaranteed that root systems are not damaged and enter grooves in an ideal vertical posture, and a foundation is laid for subsequent rapid rooting; and the V-shaped groove can be actively shaped into a more matched circular groove at the moment that the rhizome falls down, so that the gap between root soil is thoroughly eliminated.
Owner:INSTITUTE OF GRASSLAND RESEARCH OF CAAS +1

Application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis

PendingCN122074515AImprove nutrient deficienciesImprove imbalanceBiocidePlant growth regulatorsBiotechnologyCarboxylic acid
This invention provides the application and method of Streptomyces S16-1 in promoting the growth and salt stress tolerance of Leymus chinensis, belonging to the field of microbial technology. Streptomyces S16-1 was isolated from saline soil, with the preservation number CCTCC M 20251940, and its 16S rDNA sequence is shown in SEQ ID NO:1. The Streptomyces S16-1 has functional activities such as salt and alkali tolerance, phosphorus solubilization, nitrogen fixation, and production of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase. The microbial fertilizer is obtained by expanding the culture of Streptomyces S16-1. Compared with chemical fertilizers, the microbial fertilizer used in this invention is more environmentally friendly. It can not only improve soil fertility and help plants improve their salt and alkali resistance, but also promote the balance and diversity of soil microbial communities, which helps maintain the health and stability of the soil ecosystem.
Owner:INSTITUTE OF GRASSLAND RESEARCH OF CAAS

Root cutting planting method suitable for leymus chinensis seedlings in severe saline-alkali soil

The invention relates to the technical field of vegetation recovery, in particular to a leymus chinensis seedling root cutting planting method suitable for severe saline-alkali soil, which comprises the steps of seedling root cutting treatment, layered soil improvement, micro-ridge film mulching salt inhibition, transplanting and management, topdressing regulation and control and pest control. Lateral root differentiation is stimulated through root cutting, damage is repaired in cooperation with a root promoting agent, the physical and chemical properties of soil are optimized through layered improvement, the salt return phenomenon is inhibited through micro-ridge film mulching, and the survival rate and growth efficiency of seedlings are remarkably improved. According to the method, the planting period can be shortened to 12 months to form continuous vegetation coverage, the surface EC value is reduced by 62%, the pH value is stabilized below 8.5, comprehensive measures are green and efficient, the resource utilization rate is high, and the method is suitable for ecological restoration of the severe saline-alkali soil and has remarkable economic and ecological benefits.
Owner:INSTITUTE OF GRASSLAND RESEARCH OF CAAS