9 results about "Tissue expression" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to a gene for regulating plant seed size, an overexpression vector, an agrobacterium and application. The gene is BnaA03.MKK6 gene, the nucleotide sequence of which is shown as SEQ ID NO:1, the coding sequence is shown as SEQ ID NO:2, and the protein sequence is shown as SEQ ID NO:3; the plant includes Brassica napus and / or Arabidopsis thaliana. The application constructs the transgenic material of BnaA03.MKK6 and performs agronomic trait investigation, and preliminarily explores the basic function and molecular mechanism. It is found that the BnaA03.MKK6 protein is expressed on the cell nucleus and cell membrane, has tissue expression specificity, and positively regulates the plant seed size and primary root elongation. The application provides a new idea and direction for high-quality molecular breeding of Brassica napus.

The application relates to the field of biological medicine, and discloses application of REXO2 as a glioblastoma diagnosis / prognosis biomarker and a treatment target point, the expression amount of REXO2 in tumor tissues of GBM patients is higher, the patients with high expression of REXO2 have shorter survival periods, the expression amount of REXO2 increases with the increase of the malignant degree of patients, it is proved that REXO2 can be used for the diagnosis and prognosis evaluation of glioblastoma, the knockdown of REXO2 can significantly inhibit the clone formation ability of U251 cells, and the proliferation ability and tumorigenicity are reduced, which shows that REXO2 can be used as the treatment target point of glioblastoma.

This invention relates to a method and system for predicting insect multi-tissue expression levels based on multimodal feature fusion. Using the target gene as the basic prediction unit, it simultaneously inputs the target gene's regulatory sequence information, RNA stability characteristics, and protein representation information. Joint modeling is performed through a multi-tower deep learning network, outputting predicted expression levels of the target gene in multiple tissues. Advantages: It overcomes the limitations of traditional methods that primarily rely on single-modality data. For the first time, it integrates insect gene regulatory sequences, RNA stability features, and pre-trained protein language models into a unified expression prediction framework. Utilizing the multimodal prediction model, it extracts, fuses, and predicts multimodal data features, providing accurate and comprehensive gene expression level prediction results. Furthermore, it introduces a gating backoff mechanism for missing protein annotations, avoiding the misinterpretation of missing values ​​as zero or noise, thus ensuring prediction stability.

The invention belongs to the technical field of plant molecular biology, and particularly relates to a plant stem tip meristem preferential expression promoter and application thereof. A promoter sequence with preferential expression characteristics of stem tip meristem is cloned from cotton, the promoter is constructed in a GUS reporter gene vector through agrobacterium-mediated transformation, and a model plant arabidopsis thaliana is introduced for functional verification. Results show that the promoter can drive high-level GUS expression in stem tip meristem tissue of arabidopsis thaliana, presents accompanying expression in vascular related tissue such as petiole midribs and shows obvious spatial expression preference. Therefore, the promoter not only has good tissue expression selectivity, but also has cross-species recognizability and application potential.

The application of the inhibiting substance of E3 ubiquitin ligase SYVN1 in the preparation of a stomach cancer treatment drug, the small interfering RNA used in the application is aimed at the target point SYVN1, the expression level of the target point SYVN1 in the cancer tissue of a stomach cancer patient is higher than that in the cancer-adjacent tissue, and the target point SYVN1 is negatively correlated with the survival time, and has unique clinical application value.

The application discloses application of a SmCYP82D47 gene in improving resistance of eggplants to bacterial wilt, and belongs to the technical field of molecular biology. The nucleotide sequence is shown as SEQ ID NO. 2. On the basis of GWAS positioning of an eggplant bacterial wilt resistance linkage site, the application screens an eggplant bacterial wilt resistance candidate gene SmCYP82D47 through bioinformatics analysis such as gene sequence analysis and haplotype analysis; analyzes tissue expression specificity and hormone response expression characteristics of the candidate gene; and observes the disease resistance of a silencing plant and a wild type plant, and the disease resistance of a wild type plant and an overexpression plant by silencing the candidate gene in a resistant material S027 and overexpressing the candidate gene in a susceptible material S054, so as to not only verify the accuracy of the positioning result and determine the role of the candidate gene in regulating the bacterial wilt resistance of the eggplant, but also provide an important theoretical basis and technical means for eggplant disease resistance molecular breeding.

This invention discloses a gene regulating plant glandular trichome development. NtJAZ11, This application aims to address the current technical problem of unclear developmental mechanisms and lack of effective regulatory methods and utilization pathways for glandular trichomes and protective trichomes in plants (such as tobacco). The gene encoding this gene, its expression vector, and its applications are described. NtJAZ11 Overexpression and gene-editing vectors were constructed, and corresponding overexpression and gene-editing lines were obtained through transformation. Subcellular localization, tissue expression, MeJA response pattern analysis, and plant phenotypic identification showed that... NtJAZ11‑2 It can negatively regulate the development of tobacco glandular hairs, and has important application value in regulating the development of plant epidermal hairs.

This invention relates to the fields of tumor biology and cell engineering, specifically disclosing the construction and application of a human prostate cancer cell line. The human prostate cancer cell line is derived from primary prostate cancer tissue, expresses prostate-specific antigen and α-methylacyl-CoA racemic enzyme, and has a 46,XY karyotype. The construction method employs an innovative strategy of co-culturing fully digested cell suspensions with incompletely digested tissue blocks, significantly improving the success rate of establishing the cell line from the primary lesion. This cell line can stably proliferate in conventional culture media supplemented with testosterone and has been successfully applied to subcutaneous tumorigenesis models in immunodeficient mice and in vivo migration models in zebrafish. The primary lesion-derived prostate cancer cell line provided by this invention offers a highly relevant, stable, and reliable new experimental tool for studying the biological characteristics of primary prostate cancer, drug screening, and personalized treatment.