137 results about "Tissue expression" patented technology

Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants.

Described is a low voltage, pulsed electrical stimulation device for controlling expression of, for example, follistatin, a muscle formation promotion protein, by tissues. Epicardial stimulation is especially useful for heart treatment. Follistatin controlled release is also useful for treating other ailments, such as erectile dysfunction, aortic aneurysm, and failing heart valves.

A cyp71av1 gene promoter relates to the field of genetic engineering technique, the promoter sequence has a nucleotide sequence in the 2100bp of SEQ ID NO.1 in the sequence table; the promoter can lead the specific expression of the gene in the plant tentacle, and can be applied in the genetic engineering breeding using the plant tentacle tissue expression and producing metabolites. The cyp71av1 gene promoter obtained by the invention can lead the specific expression of the gene in the plant tentacle, and has great significance to the genetic engineering breeding using the plant tentacle tissue expression and producing metabolites.

The invention discloses miRNA (microribonucleic acid) biomarkers and a detection kit for ovarian cancer diagnosis. The microRNA biomarkers are composed of the following microRNAs: has-miR-193b-3p, has-miR-155-5p, has-miR-145-5p, has-miR-132-3p and has-miR-143-3p. The serological expression analysis on the five miRNAs has-miR-193b-3p, has-miR-155-5p, has-miR-145-5p, has-miR-132-3p and has-miR-143-3p of ovarian cancer with obvious para-carcinoma tissue expression differences (the differential expression level is greater than 2 folds, and the CT value in RT-PCR (reverse transcription-polymerase chain reaction) is less than 30) indicates that the five miRNAs have stable expression in serum, the serum miRNA expression has favorable consistency with the tissue, the expressions of the has-miR-193b-3p, has-miR-155-5p and has-miR-145-5p are enhanced, and the expressions of the has-miR-132-3p and has-miR-143-3p are lowered. The five miRNAs can be used as biomarkers for ovarian cancer diagnosis, and the sensitivity and specificity of combined diagnosis are obviously higher than the sensitivity and specificity of single-miRNA diagnosis.

The invention discloses a DBR2 gene promoter in the technical field of gene engineering. The promoter has an SEQ ID NO.1 1520bp nucleotide sequence in a sequence table; and the promoter can guide the gene to specifically express in plant glandular hairs and can be applied to genetic engineering breeding for plant glandular tissue expression utilization and metabolite production. The DBR2 gene promoter can guide the gene to specifically express in plant glandular hairs and is significant to the genetic engineering breeding for plant glandular tissue expression utilization and metabolite production.

The invention discloses a recombinant carp Nrf2 (NF-E2-related factor 2) gene, a recombinant carp Nrf2 protein, preparation and detection methods and application of the recombinant carp Nrf2 gene. The recombinant carp Nrf2 gene has a sequence shown by SEQIDNO:1, and the recombinant carp Nrf2 protein has a sequence shown by the SEQIDNO:2. By the nucleotide sequence of the carp Nrf2 gene complementary deoxyribonucleic acid (cDNA) disclosed by the invention, the tissue specific expression of the Nrf2 gene can be further detected through a reverse transcription-polymerase chain reaction (RT-PCR) or hybridization method, and the full-length cDNA of the carp Nrf2 gene can be further cloned. The complete Nrf2 protein may be expressed through recombining the cDNA of the recombinant carp Nrf2 gene onto an expression vector, so that the Nrf2 protein can be used for producing antibodies, and the Nrf2 gene expression states of cells, tissues, early embryos and individuals of carps under different states are detected.

The invention discloses a gene promoter, belonging to the field of plant biotechnology. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1, and the promoter is capable of regulating the specific expression of target genes in T shape trichome of a plant. Meanwhile, the invention further relates to application of the gene promoter in genetic engineering breeding which realizes expression of a gene in trichome tissues of the plant and produces metabolites. When the promoter realizing specific expression of the gene in trichome of the plant is used for carrying out genetic manipulation on the trichome system of the plant, no harm is caused to the growth or development of the plant; therefore, the promoter disclosed by the invention has an important significance for the genetic engineering breeding which realizes expression of the gene in trichome tissues of the plant and produces metabolites.

The invention relates to a promoter for regulating and controlling the expression of an AaPDR1 gene in glandular trichomes and application of the promoter. A nucleotide sequence of the promoter is shown as SEQ ID NO.1. Meanwhile, the invention also relates to a purpose of an AaPDR1 gene promoter in genetic engineering breeding for expressing and producing metabolites by plant glandular trichomes tissues. The promoter provided by the invention can guide a reporter gene to realize specific expression in plant glandular trichomes, and has important significance on the genetic engineering breeding for expressing and producing the metabolites by the plant glandular trichomes tissues.

The invention provides a method for establishing a colon cancer prognosis prediction model. The method comprises the following steps of: detecting the expression levels of SPARCL1 and P53 proteins in colon cancer tissues by immunohistochemistry; grading the tissue expression levels of the SPARCL1 and P53 proteins by a semi-quantitative method; and combining, analyzing and verifying the expression levels of the SPARCL1 and P53 proteins through a support vector machine, and finally establishing a judgment model. Immunohistochemistry detection, marker combination and support vector machine analysis are combined for establishing the colon cancer prediction model. The research shows that the model constructed by using the composition of the SPARCL1 and the P53 as a marker has experiment aid effect of predicting the prognosis of colon cancer patients. The model can be applied in postoperative transfer relapse risk prediction experiments of the colon cancer patients.

The invention discloses an miRNA biomarker and a detection kit for thyroid cancer diagnosis. The miRNA biomarker comprises microRNAs, namely hsa-miR-193b-3p, hsa-miR-384, hsa-miR-145-5p, hsa-miR-424-5p and hsa-miR-143-3p. The five miRNAs, namely the hsa-miR-193b-3p, the hsa-miR-384, the hsa-miR-424-5p, the hsa-miR-145-5p and the hsa-miR-143-3p, in which expression differences between the thyroid cancer and para-carcinoma tissues are obvious (difference expression amount is larger than 2 folds, and the CT value in RT-PCR is smaller than 30), are subjected to serologic expression analysis; results show that the five miRNAs are expressed stably in serum, expression and organization of serum miRNAs have high uniformity, expression of the hsa-miR-193b-3p, the hsa-miR-384 and the hsa-miR-145-5p is regulated down, and expression of the hsa-miR-424-5p and the hsa-miR-143-3p is regulated up. The five microRNAs can serve as the miRNA biomarker for thyroid cancer diagnosis, and sensitivity and specificity of combined diagnosis are higher than those of single miRNA diagnosis remarkably.

The invention discloses a plant tissue specific expression promoter and an application of the plant tissue specific expression promoter. A DNA fragment provided by the invention is any one of the following DNA molecules from (1)-(3): (1) a DNA molecule shown in a sequence 1 of a sequence table, (2) a DNA molecule which is hybridized with the DNA molecule in (1) under the strict condition and has the function of a promoter, and (3) a DNA molecule which has more than 90% of homology with the DNA molecule in (1) and has the function of the promoter. Experiments show that the invention provides the DNA fragment which is the promoter, can be highly expressed at roots, hypocotyl (especially the joints of leaves and the hypocotyl), and the joints of the leaves and stems of arabidopsis, and cannot be expressed in the leaves, flowers or pods. Experiments indicate that the promoter is a specific promoter which can be expressed in specific plant tissue, and can play an important role in the artificial control genetic breeding, the expression of related genes of stress resistance and stress tolerance, and the transgenic research on the cultivation of plants with the stress resistance and the stress tolerance.

The present invention discloses preparation and applications of a giant panda follicle-stimulating hormone beta subunit monoclonal antibody, wherein an anti-giant panda FSH[beta] subunit monoclonal antibody hybridoma cell line 09199 is provided, and the preservation number is CCTCC NO: C2016144. According to the present invention, a cell fusion technology is used, the hybridoma cell line secreting the anti-FSH[beat] subunit monoclonal antibody is established, the cell line culture supernatant is collected and purified, the highly-specific FSH[beta] subunit monoclonal antibody is obtained, application of the highly-specific FSH[beta] subunit monoclonal antibody in Western Blot protein detection is established to provide information for FSH protein location and tissue expression, and the deep researches on the ligand, the antagonist and the anti-antagonist of the FSH receptor are performed with the highly-specific FSH[beta] subunit monoclonal antibody so as to create the conditions for the founding of the specific ligand, the antagonist and the anti-antagonist; and the new data and the new idea are provided for the further exploration of the FSH secretion, the further regulation of the genes, and the revealing of the reproductive physiological laws.

The invention provides a rice STD1 gene and an encoding protein thereof. The sequence of the gene is shown as SEQ ID No.1. The sequence of the protein is shown as SEQ ID No.2. A mutant containing the gene has the main phenotype that the mutant is very short, cannot bear fruit and can only be stored in a heterozygous form. In addition, more cells in the mutant can be blocked in the stages S and the G2 / M, and the cell shape and the arrangement are abnormal. The STD1 mainly expresses in strongly cracking tissues. The STD1 gene directly controls the rice plant height through regulating and controlling cell cracking; the single property of short-straw gene is favorably improved; the rice STD1 gene can be used as a novel short gene resource and can be applied to rice plant height regulation and control.

The invention discloses a salivary gland tissue specific transgenic carrier and a construction method thereof. The salivary gland tissue specific transgenic carrier is obtained by emerging an upstream regulation region sequence of a mice parotid gland protein promoter and a visual screened neo-EGFP combined double-label to a piggyBac transposon system and a Lox-Cre enzyme system; the transgenic carrier can make protein express in a particular tissue to achieve a high-efficient large-section emerging efficiency. The carrier also has a green luminescence label which is convenient for screening and a neomycin resistance gene which is convenient for eukaryotic cell screening, and makes the screening of transgenic cells and identification of transgenic animals be simpler, more direct, and more accurate. The transgenic carrier provided by the invention is a salivary gland specific expression high-efficient integration universal carrier, and is capable of being applied to fields of transgenic animals such as transgenic mice and pig, and the like. The invention clones the upstream regulation region of FVB mice for the first time, the cloning method is simple and high efficient, and the difficulty in construction of a large section carrier is effectively overcome.

The invention discloses a giant panda lutropin beta subunit monoclonal antibody preparation method and an application thereof, which belong to the technical field of biology. A preservation number ofan anti-giant panda lutropin beta subunit monoclonal antibody hybridomas cell strain 14081-9 is CCTCC NO:C2018128. A cell fusion technology is used for establishing the hybridomas cell strain for secreting the anti-giant panda lutropin beta subunit monoclonal antibody, the cell strains are collected and purified to culture a supernatant, and the high-specificity lutropin beta subunit monoclonal antibody is obtained, the established high-specificity lutropin beta subunit monoclonal antibody is used for Western Blot protein detection, LH protein positioning and tissue expression information, anddeep researches on LH acceptor ligand, an antagonist, and an anti-antagonist by the LH beta submit high-specificity monoclonal antibody can be carried out, and conditions can be created for discoveryof the specific ligand, the antagonist, and the anti-antagonist. The method provides novel data for discussing LH secretion and gene further regulation and control, reveals a reproduction physiological law, reveals reproduction physiological law in giant panda, and grasps giant panda oestrus ovulation and pregnancy rules.

The invention discloses a mevalonate kinase gene in the synthetic route of ginkgo terpene lactones, an encoded protein and application thereof. The invention discloses a nucleotide sequence of the gene and the gene encoded protein sequence. Tissue expression analysis shows that ginkgo biloba mevalonate kinase gene has the highest expression in leaves and significantly low expression in flowering organs. The yeast functional complementation of the ginkgo biloba mevalonate kinase gene verifies that the ginkgo biloba mevalonate kinase gene encoded protein has the enzymatic activity of mevalonatekinase and plays a catalytic role in the synthetic route of terpene lactones. Exogenous hormone methyl jasmonate and salicylic acid are employed to treat ginkgo biloba plants, the expression of ginkgobiloba mevalonate kinase gene and the content of terpene lactones are both increased. The ginkgo biloba mevalonate kinase gene provided by the invention is in positive correlation with the content ofterpene lactones in the synthetic route of ginkgo terpene lactones, and over-expression of the gene can improve the content of terpene lactones in ginkgo biloba.

The invention discloses an FTO gene for intramuscular fat deposition. The FTO gene is an FTO (fat mass and obesity associated) gene which is separated and cloned from Banna miniature pig muscle tissue and is associated with intramuscular fat deposition. The whole length of the CDA (Complete Coding Sequence) of the gene is 1518bp and the CDS nucleotide sequence is shown as SEQ.ID.NO.1. In addition, the CDS nucleotide sequence codes 505 amino acids of which the amino acid sequence is shown as SEQ.ID.NO.2. Through the detection of a tissue expression profile of the FTO gene in seven tissues, such as pig heart, liver, spleen, lung, kidney, muscle and fat, the result shows that the FTO gene is greatly expressed in the fat tissue and the functions of the FTO gene for regulating and controlling the fat deposition in fat cells are preliminarily shown.

The invention relates to construction of an efficient double promoter PLEGFP-N1-spMyoD1 green fluorescence retrovirus vector, belonging to the technical fields of animal gene engineering and animal molecule breeding engineering. The construction of the efficient double promoter PLEGFP-N1-spMyoD1 green fluorescence retrovirus vector is characterized by artificially synthesizing a skeletal muscle specific promoter sequence, using a RT-PCR method to amplify a gene overall length sequence from a skeletal muscle tissue of a pig, designing a fusion primer, using an overlapped PCR amplification method to obtain the promoter and a fusion gene sequence, cloning T / A to a pMD18-T vector, constructing the efficient expression vector of the PLEGFP-N1-spMyoD1 double promoter, and identifying with restriction enzyme and sequencing, packaging with a liposome method and amplifying, and monitoring virus titer and efficiency of infection. The invention has the beneficial effects: 1, Primer Premier 5.0, DNA Club and Oligo 6.0 are utilized to self-design the primer; 2, the restriction that an objective gene can not be subject to specific tissue expression in the past is overcome; 3, pertinency and accuracy of study on the objective gene functions are simultaneously improved; and 4, detection is fast and convenient, and has strong reliability.

The invention belongs to the technical field of gene engineering and particularly discloses a DXR promoter for lily flower part peculiarities and wound inductions and application of the DXR promoter. The nucleotide sequence of the promoter is shown in SEQ ID NO:2. The target gene regulated by the DXR promoter is only expressed in the flower tissue in transgenic tobacco, and the expression of the target gene is related to the flower development process and is induced by damage and jasmonic acid methyl ester. In addition, the DXR promoter can be connected to any plant transformation carrier and guided into lilies or other plant cells, the transgene flowery flavor containing the promoter and resistance can be obtained, and therefore the promoter can be used for production. Peculiar molecular markers can also be generated according to promoter sequence information, used for identifying lilies or other plant flowery flavors and resistance gene types and used for molecular marker auxiliary selective breeding, and therefore the selecting efficiency of breeding is improved.

The invention discloses a promoter regulating expression of genes in non-secreted glandular hair. A nucleotide sequence of the promoter is shown as SEQ ID No:1. The invention further provides a preparation method of the promoter, a carrier with the same and an expression box. The promoter can regulate expression of target genes temporally and spatially, origination and development of the non-secreted glandular hair and metabolic regulation of secondary metabolite therein can be studied deeply, and the promoter can be applied in genetic engineering breeding for producing metabolite. Consequently, the promoter is of important significance in utilizing plant glandular hair tissue expression and genetic engineering breeding for producing the metabolite.

The invention discloses a miRNA biomarker and a detection kit which are used for renal cancer diagnosis. The microRNA biomarker is formed by the following microRNAs: hsa miR 10b 5p, hsa miR 384, hsa miR 206, hsa miR 424 5p and hsa miR 224 5p. According to the invention, serology expression analysis is carried out on five miRNAs obviously in differential expression of renal cancer and para-carcinoma tissues (differential expression quantity is more than 2 folds, and CT value in RT PCR is smaller than 30) hsa miR 10b 5p, hsa miR 384, hsa miR 424 5p, hsa miR 206 and hsa miR 224 5p, results show that the five miRNAs are stably expressed in serum, consistency between expression of serum miRNA and tissues is good, hsa miR 10b 5p, has miR 384 and has miR 206 are in down-regulated expression, and has miR 424 5p and has miR 224 5p are in up-regulated expression. The five miRNAs can serve as a biomarker used for the renal cancer diagnosis, and the sensitivity and specificity of combined diagnosis are obviously higher than those of single miRNA diagnosis.

The invention discloses an AaADH1 gene promoter and a preparation method. The nucleotide sequence of the promoter is shown in SEQ iD NO:1. The invention further relates to application of the AaADH1 gene promoter in genetic engineering breeding utilizing plant glandular hair tissue expression and metabolite production. The promoter can guide genes to be specifically expressed in plant glandular hair, due to the fact that the plant glandular hair specifically-expressed promoter carries out genetic manipulation on a plant glandular hair system, no harm is caused to growth and development of plants, and the AaADH1 gene promoter has great significance to genetic engineering breeding utilizing plant glandular hair tissue expression and metabolite production.

The invention relates to a nucleotide sequence of a cDNA code area of a goat Scp3 gene and a corresponding coded amino acid sequence, in particular to the cDNA which is used for coding a Scp3 protein and separated from goat spermary cells and an amino acid sequence corresponding to the cDNA. In the invention, a conserved region is found through comparing the known nucleotide sequences of Scp3 genes of human, rat and mouse; a pair of degenerate primers for amplifying the cDNA code area of the goat Scp3 gene with a RT-PCR method is designed without breaking amino acid coding, and the pair of the degenerate primers is amplified to form a specific fragment through the RT-PCR method; and sequencing is carried out to obtain the nucleotide sequence of the cDNA code area of the goat Scp3 gene and the amino acid sequence corresponding to the nucleotide sequence. The obtained nucleotide sequence of 708 bp can be used for detecting the tissue expression specificity of the Scp3 gene through the RT-PCR method or a hybridization method, and can be used for preparing an Scp3 antibody after a protein is obtained by recombined expression.

High affinity relaxin receptors, polypeptide compositions related thereto, as well as nucleotide compositions encoding the same, are provided. These proteins, herein termed LGR7 and LGR8, are orphan leucine-repeat-containing, G protein-coupled receptors. These receptors have a wide and a unique tissue expression pattern. The receptors, particularly soluble fragments thereof, are useful as therapeutic agents capable of inhibiting the action of relaxin and lnsL3. The receptors and fragments thereof also find use in the screening and design of relaxin agonists and antagonists. Conditions treatable with relaxin agonists or antagonists include prevention or induction of labor, treatment of endometriosis, treatment of skin conditions such as scleroderma that require collagen or extracellular matrix remodelling. Additionally, relaxin has been implicated in the dilation of blood vessels' smooth muscle cells directly and through release of nitric oxide and atrial natriuretic peptide. Relaxin has also been used in the treatment of severe chronic pain, particularly pain arising from stretching, swelling, or dislocation of tissues.

The invention discloses a longan gene DlGRAS34, a protein and an application of the longan gene DlGRAS34 and the protein in regulating and controlling flowering of plants. A cDNA nucleotide sequence of the longan gene DlGRAS34 is shown as SEQ ID No.1. The full length of an open reading frame of the longan gene DlGRAS34 is 1578bp, 525 amino acids are encoded, and the longan gene DlGRAS34 has a typical GRAS structural domain and a DELLA structure and belongs to GRAS protein. A qRT-PCR result shows that the gene has tissue expression specificity, has the highest relative expression quantity in pericarp, and has the second relative expression quantity in stem, leaf and flower organs; expression is down-regulated in the early flowering induction stage of 'Sijimi' longan, and expression is up-regulated in the later flowering stage of 'Shixia' longan. A transient expression result of the arabidopsis protoplast shows that a fluorescence signal is mainly concentrated on a cell nucleus. A transgenic arabidopsis thaliana result shows that a DlGRAS34-overexpressed transgenic plant shows a late flowering phenomenon that a wild type plant blooms in about 27 days, and a transgenic line blooms in 32-35 days; and meanwhile, rosette leaves become large, and plants become short. The results show that as a typical transcription factor, the protein coded by the DlGRAS34 is located in a cell nucleus, plant flowering is negatively regulated, and the overexpression of the DlGRAS34 gene can significantly inhibit plant flowering.

The invention provides an AaDBR2 gene promoter DBR2pro1 and applications of the AaDBR2 gene promoter DBR2pro1, belonging to the technical field of genetic engineering. The nucleotide sequence of the promoter is shown as SEQ ID NO.1. The invention further relates to the applications of the AaDBR2 gene promoter DBR2pro1 in genetic engineering breeding utilizing plant secretion glandular hair tissue expression and production metabolites. The AaDBR2 gene promoter DBR2pro1 provided by the invention can guide the exogenous gene to be specifically expressed in the plant secretion glandular hair, thus having great significance in the genetic engineering breeding utilizing plant secretion glandular hair tissue expression and production metabolites.

The invention discloses a method for detecting ferric-chelate reductase family genes of Citrus reticulata Blanco. The method comprises 1) selecting one-year-old seedlings of Ziyang orange, Carrizo citrange and trifoliate orange as tissue expression analysis materials, 2) extracting RNAs of the root, stem and leaf tissue of the seedlings, 3) carrying out inverse transcription synthesis through the RNAs as templates to obtain a first chain of cDNA, 4) carrying out fluorescence quantitative PCR, and 5) carrying out analysis on the test data through a 2 delta Ct method. The method realizes identification of expression conditions of five types of ferric-chelate reductase genes in different parts of Citrus reticulata Blanco through the specific primers and reaction system and is beneficial to research Citrus reticulata Blanco resistance to iron deficiency.

The invention relates to the technical field of biology, in particular to a seed-specific promoter sequence separated from soybean and applications thereof. The nucleotide sequence is shown in SEQ No.1. The seed-specific promoter sequence is used as the seed-specific promoter of soybean. In the invention, the stearic acid-acyl carrier protein (ACP) desaturase promoter of soybean is cloned and the tissue expression characteristics and expression efficiency of the promoter in soybean are researched, thus an evidence for the effective use of the promoter is provided; and the promoter provides a good tool for the transgenic research of soybean and creates conditions for the researches and developments by utilizing the soybean seed as bioreactor.