32 results about "Tissue expression" patented technology

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-human carbohydrate antigen CA125 monoclonal antibody and application thereof. The amino acid sequences of CDR1-3 on a light chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.3-5, and the amino acid sequences of CDR1-3 on a heavy chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.8-10. The monoclonal antibody provided by the invention can specifically recognize and bind to a natural carbohydrate antigen CA125 expressed by human cells or tissues, has good affinity and relatively high sensitivity of binding of the antibody and the antigen, has strong anti-interference ability to complex non-target protein components in the cells / tissues, is beneficial to significantly improving the accuracy and reliability of immunodetection of human CA125 protein, and has good application prospects. The method is suitable for high-specificity, high-sensitivity and high-reliability detection of the human CA125 protein, and has good applicability in a plurality of detection systems such as immunoblotting, immunoprecipitation, immunohistochemistry and the like.

The invention provides a disease marker epitope prediction and antibody screening method, which belongs to the technical field of disease markers, and comprises the following steps: firstly, establishing a training data set containing a known antigen-antibody compound structure and disease tissue expression data; and obtaining target protein sequence information through liquid chromatography-mass spectrometry analysis and carrying out sequence comparison. And then a deep convolutional neural network is utilized to extract sequence features, and surface exposure sites are identified by combining secondary structure prediction and solvent accessibility analysis. After the features are integrated with sequence evolution conservative properties, a prediction model is constructed by using a random forest classifier. The method comprises the following steps: carrying out molecular dynamics simulation on a prediction result, screening first 10% of candidate sequences through a comprehensive scoring function and K-means clustering, and finally determining an antigen epitope sequence with the strongest binding activity through verification of an antigen chip and a fluorescence labeled antibody system, so that the technical problem that specific antigen epitopes are difficult to accurately predict and recognize in the prior art is solved.

The invention discloses application of a SmCYP82D47 gene in improvement of eggplant bacterial wilt resistance, and belongs to the technical field of molecular biology. 1, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 2. On the basis of positioning an eggplant bacterial wilt resistance linkage site by GWAS, the eggplant bacterial wilt resistance candidate gene SmCYP82D47 is screened through bioinformatics analysis such as gene sequence analysis and haplotype analysis; the tissue expression specificity, hormone response expression characteristics and the like of candidate genes are analyzed; by silencing candidate genes in a resistant material S027 and overexpressing the candidate genes in a susceptible material S054, the disease resistance conditions of a silent plant and a wild type plant as well as the wild type plant and an overexpressed plant are observed, so that the accuracy of a positioning result can be verified, and the effect of the candidate genes in regulating and controlling the bacterial wilt resistance of the eggplant is determined; important theoretical basis and technical means can be provided for eggplant disease-resistant molecular breeding.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The invention discloses application of an mRNAs marker in early diagnosis of lung signet-ring cell carcinoma and a kit. The marker is a combination of C1QA, CRISPLD2, ROBO4, SPARCL1, SFTPB, LAMC3, FN1, EMP1, CD68, THBS1, PRG4, ICAM1, PODXL, KDM6B, ENPP2, KLF10 and THBD (tetrahydrofuran). According to the invention, FFPE cancer tissue of a patient with primary pulmonary signet ring cell carcinoma diagnosed by an existing detection means in clinic is used as a sample, para-carcinoma tissue is used as a control sample, mRNA expression profiles of the cancer tissue and the control tissue are detected by adopting an RNA sealing technology, and 17 differentially expressed mRNA markers are found by applying statistics and a machine learning method. Based on the 17 found markers, a risk scoring model with high sensitivity and specificity is constructed, and the sensitivity gt of the model to early-stage lung signet-ring cell carcinoma diagnosis; the specificity is gt; 89%.

The present invention belongs to the field of antibody technology, and in particular relates to monoclonal antibodies, antibody conjugates and applications thereof against p21 proteins. The amino acid sequences of the complementary determining regions CDR1-3 on the light chain variable region of the monoclonal antibody are shown as SEQ ID NO.3-5, respectively; the amino acid sequences of the complementary determining regions CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.8-10, respectively. The monoclonal antibody provided by the present invention can specifically recognize p21 proteins expressed by human cells and tissues, has good affinity for binding to p21 proteins, high recognition sensitivity, and strong anti-interference ability, is suitable for immunodiagnostic analysis of p21 proteins in tissues / cells, and has broad application prospects and good market prospects in clinical diagnosis and scientific research detection. In addition, the antibody has cross-reactivity to homologous p21 proteins of humans, rats and mice, and has certain applicability to the detection of rat and mouse p21 proteins.

The invention discloses a biomarker for monitoring the curative effect of a PD-1 antibody of a PD-L1Positive gastric cancer patient, and relates to the technical field of biomedical detection, and the biomarker is laminin gamma 2. According to the invention, the laminin gamma2 is used as a novel biomarker for monitoring the curative effect of the PD-1 treatment antibody of the PD-L1Positive gastric cancer patient, experiments prove that the serum level of the laminin gamma2 is higher than that of healthy people in the gastric cancer patient and is in positive correlation with a prognosis related factor sPD-L1, the tissue expression of the PD-L1Positive patient is higher, and the serum level of a person without PD-1 antibody treatment response is higher; it is proved that the marker has good progress monitoring value, treatment reaction differences can be effectively distinguished, and the blank of lack of PD-1 antibody curative effect monitoring markers for patients of the type is filled up.

The invention discloses a structure-driven single cell transcriptomics immune cell annotation system and method, and the system comprises a data loading and preprocessing module which is used for loading a marker table and data after single cell clustering, and extracting the expression characteristic statistic of each cluster from the marker table and data to form a characteristic matrix; the basic structure annotation module is used for carrying out preliminary structure annotation on peripheral blood mononuclear cells and tissue samples; the tissue extension annotation module is used for completing structure extension and refinement under tissue expression drift on the basis of preliminary structure annotation according to a sample structure annotation requirement from peripheral tissues; and the structure dimension auxiliary annotation module is configured to integrate the dimension reduction coordinates and the adjacency graph information after being triggered, and perform spatial consistency verification and auxiliary correction on the low-confidence annotation result. According to the method, an automatic annotation function with high accuracy, strong controllability and good interpretability can be realized, and the method has high interpretability and robustness.

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.

The invention discloses an adeno-associated virus overexpressing GPR110 and application of the adeno-associated virus in preparation of a medicine for treating metabolism-related steatohepatitis. The adeno-associated virus comprises a GPR110 gene sequence encoded and operably connected to a liver specific promoter TBG, an in-vivo experiment is carried out in a constructed MASH mouse model, and the result shows that the expression of the GPR110 gene in the liver tissue of the MASH mouse model is reduced, so that the GPR110 gene can be used as a biomarker and a therapeutic target of MASH, and the application of the GPR110 gene in the MASH mouse model is proven. According to the present invention, the MASH-related symptoms can be significantly improved through the AAV adeno-associated virus targeting liver specific overexpression GPR110, wherein the MASH-related symptoms comprise liver triglyceride content reducing, liver injury relieving, liver inflammation improving and liver fibrosis improving; the AAV-mediated GPR110 overexpression can effectively improve the MASH pathological process, and new intervention drugs and means can be provided for MASH treatment.

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-human prostate specific membrane antigen (PSMA) antibody and application thereof. The amino acid sequences of CDR1-3 on a light chain variable region of the antibody are respectively shown as SEQ ID NO.3-5, and the amino acid sequences of CDR1-3 on a heavy chain variable region of the antibody are respectively shown as SEQ ID NO.8-10. The antibody with the complementarity determining region sequence provided by the invention can specifically recognize PSMA expressed by cells and tissues, has good affinity in combination with the PSMA, has high recognition sensitivity, can effectively resist the interference of complex non-target protein components in the cells / tissues, has no specific combination with non-target antigens, is suitable for immunological detection of the PSMA, and has good application prospects. The kit is especially suitable for western blot detection and immunohistochemical methods, and can reduce the occurrence rate of false positive and false negative results and improve the accuracy of immunodiagnosis.

The application discloses a kind of ZmDAR1b protein in the method for regulating corn height, comprising the following steps: S1 cloning corn gene ZmDAR1b;S2 analysis corn gene ZmDAR1b's tissue expression pattern;S3 create corn ZmDAR1b gene editing knockout mutant.The ZmDAR1b protein described in the application, regulate protein activity or content or regulate ZmDAR1b protein coding gene expression substance, can regulate plant height, stem length, cell expansion, kernel size.The application obtains the transgenic knockout mutant material of ZmDAR1b by gene editing technology and corn genetic transformation technology, and carries out multi-year multi-point field test and observation, and the biological function of ZmDAR1b gene in corn is determined, which provides important gene resources for corn excellent variety breeding, and has very important application value.

The invention relates to the technical field of medical detection. The invention aims to provide a nano-probe for detecting the expression of an epidermal growth factor receptor of head and neck squamous carcinoma cells. The nano-probe is a nano-particle with a core-shell structure, the nano particle takes a nano Au particle as a core, and 4-mercaptobenzoic acid (4-MBA) is adsorbed on the surface of the Au core as a Raman signal internal reference molecule; according to the nanoparticle, SiO2 is used as a shell, and PEG and EGFR antibodies are linked to the SiO2 shell. According to the nanoprobe, head and neck squamous cell cancer cell and tissue imaging is researched by adopting a surface enhanced Raman spectroscopy technology, tumor cell and tissue EGFR expression images are drawn, and quantitative analysis and research are carried out. A rapid, visual and accurate new tool is provided for clinically detecting the squamous cell carcinoma of the head and neck and the EGFR expression condition of the squamous cell carcinoma of the head and neck.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The invention discloses an mRNAs marker and a kit for early diagnosis of pulmonary signet-ring cell carcinoma. The marker is a combination of FASN, RGPD2, ACSL1, ABCA3, MT2A, ITPKC, FKBP5, CEACAM6, HP, CAPN8, FCGBP and MUC16, and is characterized in that the marker is a combination of FASN, RGPD2, ACSL1, ABCA3, MT2A, ITPKC, FKBP5, CEACAM6, HP, CAPN8 and MUC16. According to the invention, FFPE cancer tissue of a patient with primary pulmonary signet-ring cell carcinoma diagnosed by an existing detection means in clinic is used as a sample, para-carcinoma tissue is used as a control sample, mRNA expression profiles of the cancer tissue and the control tissue are detected by adopting an RNA sealing technology, and 12 differentially expressed mRNA markers are found by applying statistics and machine learning methods. Based on the found 12 markers, a risk scoring model with high sensitivity and specificity is constructed, and the sensitivity gt of the model to early-stage lung signet-ring cell carcinoma diagnosis; the specificity is gt; 60%.

The invention provides garlic-derived exosome-like nano-vesicles as well as an extraction method and application thereof in acute lung injury, and belongs to the technical field of biological medicines. The invention provides an extraction method of garlic-derived exosome-like nano-vesicles, which comprises the following steps: adjusting to five times of centrifugation, sequentially increasing centrifugal force, and after the five times of centrifugation, precipitate contains the garlic-derived exosome-like nano-vesicles. By utilizing the garlic-derived exosome-like nano-vesicle, the content of inflammatory factors in mouse alveolar lavage fluid and lung tissues can be reduced, and the mRNA expression level can be reduced; the mouse lung tissue expression of cGAS, Sting, TBK1, p-TBK1, IRF3, p-IRF3, p65 and p-p65 is reduced, the cGAS / Stin / NF-kappa B signal channel is regulated, and the occurrence and development of acute lung injury are effectively retarded.

The application belongs to the technical field of genetic engineering, and particularly relates to a gene for regulating plant seed size, an overexpression vector, an agrobacterium and application. The gene is BnaA03.MKK6 gene, the nucleotide sequence of which is shown as SEQ ID NO:1, the coding sequence is shown as SEQ ID NO:2, and the protein sequence is shown as SEQ ID NO:3; the plant includes Brassica napus and / or Arabidopsis thaliana. The application constructs the transgenic material of BnaA03.MKK6 and performs agronomic trait investigation, and preliminarily explores the basic function and molecular mechanism. It is found that the BnaA03.MKK6 protein is expressed on the cell nucleus and cell membrane, has tissue expression specificity, and positively regulates the plant seed size and primary root elongation. The application provides a new idea and direction for high-quality molecular breeding of Brassica napus.

The application relates to the field of biological medicine, and discloses application of REXO2 as a glioblastoma diagnosis / prognosis biomarker and a treatment target point, the expression amount of REXO2 in tumor tissues of GBM patients is higher, the patients with high expression of REXO2 have shorter survival periods, the expression amount of REXO2 increases with the increase of the malignant degree of patients, it is proved that REXO2 can be used for the diagnosis and prognosis evaluation of glioblastoma, the knockdown of REXO2 can significantly inhibit the clone formation ability of U251 cells, and the proliferation ability and tumorigenicity are reduced, which shows that REXO2 can be used as the treatment target point of glioblastoma.

The invention discloses application of siRNA of silence Ier3 gene in preparation of medicine for preventing or treating meibomian gland dysfunction type dry eye, Ier3 gene expression is regulated and controlled through RNA interference technology, and meibomian gland dysfunction in plateau environment is relieved. The invention discovers that the expression of the early response gene Ier3 in hypoxia-induced meibomian gland dysfunction is up-regulated for the first time, and designs and synthesizes three specific siRNA sequences. Experiments adopt a low-pressure oxygen chamber to simulate an altitude of 5000 meters to construct a plateau mouse model, intervention is performed through an eye drop administration mode, and results show that Ier3 siRNA can significantly inhibit meibomian gland tissue Ier3 expression, prolong tear film rupture time and inhibit meibomian gland duct cell proliferation. The invention provides a precise treatment strategy based on the RNA interference technology, has the characteristics of strong targeting property, high gene silencing efficiency and small side effect, and provides a new intervention target and drug selection for the treatment of the plateau environment related meibomian gland dysfunction type dry eye.

This invention discloses mRNA markers and kits for the early diagnosis of lung signet ring cell carcinoma. The markers are a combination of FASN, RGPD2, ACSL1, ABCA3, MT2A, ITPKC, FKBP5, CEACAM6, HP, CAPN8, FCGBP, and MUC16. This invention uses FFPE cancer tissue from patients clinically diagnosed with primary lung signet ring cell carcinoma using existing detection methods as samples, and adjacent normal tissue as a control sample. RNA sequencing technology is used to detect the mRNA expression profiles of cancer tissue and control tissue. Statistical and machine learning methods are applied to identify 12 differentially expressed mRNA markers. Based on these 12 markers, a risk scoring model with high sensitivity and specificity is constructed. The model has a sensitivity >80% and a specificity >60% for the diagnosis of early lung signet ring cell carcinoma.

This invention relates to a method and system for predicting insect multi-tissue expression levels based on multimodal feature fusion. Using the target gene as the basic prediction unit, it simultaneously inputs the target gene's regulatory sequence information, RNA stability characteristics, and protein representation information. Joint modeling is performed through a multi-tower deep learning network, outputting predicted expression levels of the target gene in multiple tissues. Advantages: It overcomes the limitations of traditional methods that primarily rely on single-modality data. For the first time, it integrates insect gene regulatory sequences, RNA stability features, and pre-trained protein language models into a unified expression prediction framework. Utilizing the multimodal prediction model, it extracts, fuses, and predicts multimodal data features, providing accurate and comprehensive gene expression level prediction results. Furthermore, it introduces a gating backoff mechanism for missing protein annotations, avoiding the misinterpretation of missing values ​​as zero or noise, thus ensuring prediction stability.

The invention belongs to the technical field of plant molecular biology, and particularly relates to a plant stem tip meristem preferential expression promoter and application thereof. A promoter sequence with preferential expression characteristics of stem tip meristem is cloned from cotton, the promoter is constructed in a GUS reporter gene vector through agrobacterium-mediated transformation, and a model plant arabidopsis thaliana is introduced for functional verification. Results show that the promoter can drive high-level GUS expression in stem tip meristem tissue of arabidopsis thaliana, presents accompanying expression in vascular related tissue such as petiole midribs and shows obvious spatial expression preference. Therefore, the promoter not only has good tissue expression selectivity, but also has cross-species recognizability and application potential.

The application of the inhibiting substance of E3 ubiquitin ligase SYVN1 in the preparation of a stomach cancer treatment drug, the small interfering RNA used in the application is aimed at the target point SYVN1, the expression level of the target point SYVN1 in the cancer tissue of a stomach cancer patient is higher than that in the cancer-adjacent tissue, and the target point SYVN1 is negatively correlated with the survival time, and has unique clinical application value.

The application discloses application of a SmCYP82D47 gene in improving resistance of eggplants to bacterial wilt, and belongs to the technical field of molecular biology. The nucleotide sequence is shown as SEQ ID NO. 2. On the basis of GWAS positioning of an eggplant bacterial wilt resistance linkage site, the application screens an eggplant bacterial wilt resistance candidate gene SmCYP82D47 through bioinformatics analysis such as gene sequence analysis and haplotype analysis; analyzes tissue expression specificity and hormone response expression characteristics of the candidate gene; and observes the disease resistance of a silencing plant and a wild type plant, and the disease resistance of a wild type plant and an overexpression plant by silencing the candidate gene in a resistant material S027 and overexpressing the candidate gene in a susceptible material S054, so as to not only verify the accuracy of the positioning result and determine the role of the candidate gene in regulating the bacterial wilt resistance of the eggplant, but also provide an important theoretical basis and technical means for eggplant disease resistance molecular breeding.

According to the technical route of prokaryotic expression, protein purification and enzyme activity verification, novel synthase AaTPS19 in artemisia apiacea is found, GPP is catalyzed by the novel synthase AaTPS19 to generate 2-Pinanol and Geraniol, the novel synthase AaTPS19 is the first identified 2-PinanolSynthase, the product type of the monoterpene synthase is broadened, potential functions and evolution characteristics of the monoterpene synthase are analyzed, and the novel synthase AaTPS19 has the advantages that the novel synthase AaTPS19 has the advantages that the novel synthase AaTPS19 has the advantages that the novel synthase AaTPS19 is used for catalyzing GPP to generate the 2-Pinanol and the Geraniol; and a tissue expression mode of the gene is obtained through fluorescent quantitative PCR, so that a foundation is laid for prokaryotic expression and in-vitro functional verification of the TPS.

This invention discloses a gene regulating plant glandular trichome development. NtJAZ11, This application aims to address the current technical problem of unclear developmental mechanisms and lack of effective regulatory methods and utilization pathways for glandular trichomes and protective trichomes in plants (such as tobacco). The gene encoding this gene, its expression vector, and its applications are described. NtJAZ11 Overexpression and gene-editing vectors were constructed, and corresponding overexpression and gene-editing lines were obtained through transformation. Subcellular localization, tissue expression, MeJA response pattern analysis, and plant phenotypic identification showed that... NtJAZ11‑2 It can negatively regulate the development of tobacco glandular hairs, and has important application value in regulating the development of plant epidermal hairs.

This invention discloses the application of mRNA biomarkers in the early diagnosis of lung signet ring cell carcinoma and a kit. The biomarkers are a combination of C1QA, CRISPLD2, ROBO4, SPARCL1, SFTPB, LAMC3, FN1, EMP1, CD68, THBS1, PRG4, ICAM1, PODXL, KDM6B, ENPP2, KLF10, and THBD. This invention uses FFPE cancer tissue from patients clinically diagnosed with primary lung signet ring cell carcinoma using existing detection methods as samples, and adjacent normal tissue as control samples. RNA sequencing technology is used to detect the mRNA expression profiles of cancer tissue and control tissue. Statistical and machine learning methods are applied to identify 17 differentially expressed mRNA biomarkers. Based on these 17 biomarkers, a risk scoring model with high sensitivity and specificity is constructed. The model has a sensitivity >75% and a specificity >89% for the diagnosis of early lung signet ring cell carcinoma.

The invention relates to the technical field of biological medicines, and particularly discloses application of Metrnl as a cisplatin renal injury marker and a cisplatin renal injury prevention and treatment medicine. The Metrnl level change in cisplatin-induced acute and chronic renal injury models is researched, and the results show that the Metrnl protein level of model animal blood is increased, and the Metrnl expression of renal tissues is reduced. It is proved for the first time that METRNL can be used as a diagnosis marker of cisplatin-induced renal injury. Metrnl knockout and overexpression experiments prove that knockout of Metrnl reduces the proliferation activity of HK-2 cells under cis-platinum stimulation, aggravates cell apoptosis and aggravates renal injury induced by cis-platinum; through metrnl overexpression, HK-2 cell injury and inflammation levels under cis-platinum stimulation can be relieved, and cis-platinum induced renal injury can be relieved. Metrnl has the effect of preventing and / or treating the cisplatin renal injury.

The present invention provides methods for treating, reducing the severity, or inhibiting the growth of cancer (e.g., lung cancer). The methods of the present invention comprise administering a therapeutically effective amount of a programmed death 1 (PD-1) antagonist (e.g., an anti-PD-1 antibody), to a subject with lung cancer wherein the cancer tissue expresses PD-L1.

The invention discloses an OsNAC028 gene for regulating and controlling plant salt tolerance and application of the OsNAC028 gene, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the OsNAC028 gene for regulating and controlling the salt tolerance of the plants is shown as SEQ ID NO.1, and the amino acid sequence of the OsNAC028 gene for regulating and controlling the salt tolerance of the plants is shown as SEQ ID NO.2. The gene function is analyzed through methods of subcellular localization, tissue expression quantity detection, OsNAC028 response salt stress induction detection, OsNAC028 gene overexpression plant and mutant knockout plant salt stress phenotype identification and the like, it is proved that the OsNAC028 gene can positively regulate the plant salt stress response process, the expression quantity of the OsNAC028 gene in a plant is increased, and the salt tolerance of the plant can be improved; and theoretical and production practice foundations are laid for cultivating new varieties of salt-resistant transgenic crops.