67 results about "Tissue expression" patented technology

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-human carbohydrate antigen CA125 monoclonal antibody and application thereof. The amino acid sequences of CDR1-3 on a light chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.3-5, and the amino acid sequences of CDR1-3 on a heavy chain variable region of the monoclonal antibody are respectively shown as SEQ ID NO.8-10. The monoclonal antibody provided by the invention can specifically recognize and bind to a natural carbohydrate antigen CA125 expressed by human cells or tissues, has good affinity and relatively high sensitivity of binding of the antibody and the antigen, has strong anti-interference ability to complex non-target protein components in the cells / tissues, is beneficial to significantly improving the accuracy and reliability of immunodetection of human CA125 protein, and has good application prospects. The method is suitable for high-specificity, high-sensitivity and high-reliability detection of the human CA125 protein, and has good applicability in a plurality of detection systems such as immunoblotting, immunoprecipitation, immunohistochemistry and the like.

The invention discloses a polygonatum cyrtonema transcription factor gene PcWRKY1 as well as an encoding protein and application thereof. According to the application, a WRKY transcription factor PcWRKY1 which is obviously inhibited by high-temperature stress is screened, the transcription level of the PcWRKY1 transcription factor gene responding to the high-temperature stress is further determined, a tissue expression mode and subcellular sites of the PcWRKY1 transcription factor gene are analyzed, and finally, the regulation effect of the PcWRKY1 transcription factor gene on the high-temperature tolerance of the polygonatum cyrtonema protoplast is determined. The PcWRKY1 transcription factor gene is located in a cell nucleus, codes a protein containing 519 amino acids and is expressed in roots, stems, leaves, tubers and seeds of polygonatum cyrtonema, the expression quantity in the seeds is the highest, and the PcWRKY1 transcription factor gene can inhibit the increase of MDA content and promote the improvement of CAT enzyme activity under high temperature stress. Further, the tolerance of the polygonatum cyrtonema protoplast under high-temperature stress is improved.

The present invention belongs to the field of genetic engineering and biotechnology, and relates to the screening, construction and application of a modified type of AAV-8 serotype for gene targeting and expression. The modified type of the AAV-8 serotype of the present invention inserts 10 amino acid sequences between the 590th amino acid and the 591st amino acid of the AAV-8 serotype capsid protein sequence as shown in SEQ ID NO.2, wherein the 3rd to 9th amino acids are amino acid sequences obtained by screening. The modified serotype of the present invention targets each layer of the retina, and the fluorescence effect after infection is stronger, and it can be clearly and intuitively observed that the exogenous gene can be efficiently expressed in each layer of the retina of the targeted tissue, including the retinal ganglion cell layer, the inner nuclear layer, the outer nuclear layer, the photoreceptor cell layer and the retinal pigment epithelial cell layer, and has better and more targeted tissue expression than the wild-type serotype when the same amount of virus is used.

The invention belongs to the technical field of gene engineering and biology, discloses a modified adeno-associated virus AAV-8 serotype for retina wide targeting and expression, and further relates to construction and application of the modified type. 10 amino acid sequences as shown in SEQ ID NO.3 are inserted between the 590th amino acid and the 591th amino acid of an AAV-8 serotype capsid protein sequence as shown in SEQ ID NO.2, the first, second and tenth amino acids are protected amino acids, and the third to ninth amino acids are amino acid sequences obtained by screening. The modified serotype disclosed by the invention is stronger in fluorescence effect after infection and can be obviously and intuitively observed, an exogenous gene can be widely and efficiently expressed in a specific targeted tissue retina whole region, and the expression efficiency is greatly improved under the condition of using the same virus amount. And compared with a wild serotype, the serotype has better and wider targeted tissue expression and less non-targeted tissue expression.

The invention discloses a plant glandular hair development regulation gene NtJAZ11 as well as an encoding protein, an expression vector and application thereof, and aims to solve the technical problems that at present, different development mechanisms for glandular hair and protection hair of plants (such as tobacco) are unknown, and effective regulation means and utilization ways are lacked. A gene NtJAZ11 capable of regulating glandular hair and protecting hair development is identified, an overexpression vector and a gene editing vector of the gene NtJAZ11 are constructed, and a corresponding overexpression strain and a corresponding gene editing strain are obtained through transformation; subcellular localization, tissue expression, MeJA response mode analysis, plant phenotypic characteristic identification and the like show that NtJAZ11-2 can negatively regulate and control the development of tobacco glandular hair, and has important application value in regulating and controlling the development process of plant epidermal hair.

The invention relates to the technical field of image processing, in particular to a diabetic cognitive function obstruction detection method based on an MRI (Magnetic Resonance Imaging) image, which comprises the following steps: acquiring a T1 weighted image and a T2 weighted image; obtaining brain pixel points according to the positions of the pixel points in the two weighted images; according to the gray expression of the brain pixel points in the two weighted images, the possibility that the brain pixel points are brain quality pixel points is obtained, and the tissue expression degree of each pixel point in the two weighted images is further obtained; according to the pixel points of the brain pixel points in the two weighted images, combining the tissue expression degree of each pixel point in the two weighted images to obtain the edge features of the brain pixel points; and de-noising the two weighted images according to the edge features of the brain pixel points and the possibility that the brain pixel points are brain quality pixel points. According to the method, the gray level expressions of the pixel points in the two weighted images are analyzed, the unique features of the noise are combined, and smoothing parameters are given to each pixel point, so that the loss of detail features is avoided.

The invention provides a disease marker epitope prediction and antibody screening method, which belongs to the technical field of disease markers, and comprises the following steps: firstly, establishing a training data set containing a known antigen-antibody compound structure and disease tissue expression data; and obtaining target protein sequence information through liquid chromatography-mass spectrometry analysis and carrying out sequence comparison. And then a deep convolutional neural network is utilized to extract sequence features, and surface exposure sites are identified by combining secondary structure prediction and solvent accessibility analysis. After the features are integrated with sequence evolution conservative properties, a prediction model is constructed by using a random forest classifier. The method comprises the following steps: carrying out molecular dynamics simulation on a prediction result, screening first 10% of candidate sequences through a comprehensive scoring function and K-means clustering, and finally determining an antigen epitope sequence with the strongest binding activity through verification of an antigen chip and a fluorescence labeled antibody system, so that the technical problem that specific antigen epitopes are difficult to accurately predict and recognize in the prior art is solved.

The invention relates to the technical field of biology, and discloses application of a cucumber CsTOR gene in cucumber grafted seedlings, and the cucumber CsTOR gene has high expression level in tender tissues of root tips, stem tips and male flower buds of cucumbers; subcells of the cucumber CsTOR gene are localized in a cell nucleus and a cell membrane; the cucumber CsTOR gene is used for regulating and controlling the survival rate of cucumber grafted seedlings. The cucumber-pumpkin grafted seedlings treated by a control group and the TOR inhibitor are further subjected to transcriptome and metabolome analysis, and it is found that after the TOR kinase activity is inhibited, the content of plant hormone metabolism related genes and hormones is remarkably changed, especially auxin. The CsTOR-mediated auxin-regulated grafting interface healing mechanism research is preliminarily disclosed, and while the research on the heterologous grafting interface healing mechanism is enriched, theoretical support is provided for creating a rapid healing material and optimizing a grafted cucumber production technology in the future.

The invention discloses application of a SmCYP82D47 gene in improvement of eggplant bacterial wilt resistance, and belongs to the technical field of molecular biology. 1, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 2. On the basis of positioning an eggplant bacterial wilt resistance linkage site by GWAS, the eggplant bacterial wilt resistance candidate gene SmCYP82D47 is screened through bioinformatics analysis such as gene sequence analysis and haplotype analysis; the tissue expression specificity, hormone response expression characteristics and the like of candidate genes are analyzed; by silencing candidate genes in a resistant material S027 and overexpressing the candidate genes in a susceptible material S054, the disease resistance conditions of a silent plant and a wild type plant as well as the wild type plant and an overexpressed plant are observed, so that the accuracy of a positioning result can be verified, and the effect of the candidate genes in regulating and controlling the bacterial wilt resistance of the eggplant is determined; important theoretical basis and technical means can be provided for eggplant disease-resistant molecular breeding.

The present invention discloses an anti-giant panda LIF monoclonal antibody, a hybridoma cell line, and use thereof, and belongs to the field of biotechnology. The antibody comprises a heavy chain variable region having an amino acid sequence set forth in SEQ ID NO. 1 and a light chain variable region having an amino acid sequence set forth in SEQ ID NO. 2. The hybridoma cell line LIF-2 is deposited at China Center for Type Culture Collection on Oct. 28, 2021 with the accession number of CCTCC NO: C202171. The anti-giant panda LIF monoclonal antibody developed by the present invention can accurately identify the target protein LIF, and lays an important foundation for LIF protein localization, tissue expression information, study on LIF and a receptor and a target cell thereof by using the high-specificity LIF monoclonal antibody and exploration of the effect of LIF in embryonic diapause in giant pandas.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The invention discloses application of an mRNAs marker in early diagnosis of lung signet-ring cell carcinoma and a kit. The marker is a combination of C1QA, CRISPLD2, ROBO4, SPARCL1, SFTPB, LAMC3, FN1, EMP1, CD68, THBS1, PRG4, ICAM1, PODXL, KDM6B, ENPP2, KLF10 and THBD (tetrahydrofuran). According to the invention, FFPE cancer tissue of a patient with primary pulmonary signet ring cell carcinoma diagnosed by an existing detection means in clinic is used as a sample, para-carcinoma tissue is used as a control sample, mRNA expression profiles of the cancer tissue and the control tissue are detected by adopting an RNA sealing technology, and 17 differentially expressed mRNA markers are found by applying statistics and a machine learning method. Based on the 17 found markers, a risk scoring model with high sensitivity and specificity is constructed, and the sensitivity gt of the model to early-stage lung signet-ring cell carcinoma diagnosis; the specificity is gt; 89%.

The present invention belongs to the field of antibody technology, and in particular relates to monoclonal antibodies, antibody conjugates and applications thereof against p21 proteins. The amino acid sequences of the complementary determining regions CDR1-3 on the light chain variable region of the monoclonal antibody are shown as SEQ ID NO.3-5, respectively; the amino acid sequences of the complementary determining regions CDR1-3 on the heavy chain variable region are shown as SEQ ID NO.8-10, respectively. The monoclonal antibody provided by the present invention can specifically recognize p21 proteins expressed by human cells and tissues, has good affinity for binding to p21 proteins, high recognition sensitivity, and strong anti-interference ability, is suitable for immunodiagnostic analysis of p21 proteins in tissues / cells, and has broad application prospects and good market prospects in clinical diagnosis and scientific research detection. In addition, the antibody has cross-reactivity to homologous p21 proteins of humans, rats and mice, and has certain applicability to the detection of rat and mouse p21 proteins.

The invention discloses a biomarker for monitoring the curative effect of a PD-1 antibody of a PD-L1Positive gastric cancer patient, and relates to the technical field of biomedical detection, and the biomarker is laminin gamma 2. According to the invention, the laminin gamma2 is used as a novel biomarker for monitoring the curative effect of the PD-1 treatment antibody of the PD-L1Positive gastric cancer patient, experiments prove that the serum level of the laminin gamma2 is higher than that of healthy people in the gastric cancer patient and is in positive correlation with a prognosis related factor sPD-L1, the tissue expression of the PD-L1Positive patient is higher, and the serum level of a person without PD-1 antibody treatment response is higher; it is proved that the marker has good progress monitoring value, treatment reaction differences can be effectively distinguished, and the blank of lack of PD-1 antibody curative effect monitoring markers for patients of the type is filled up.

The present invention relates to a nucleic acid encoding ATP7B, an adeno-associated virus vector, and a use thereof in preparing a drug for preventing and treating Wilson's disease. Provided is an ATP7B GOI vector that can be packaged into an adeno-associated virus having cellular activity, relatively high yield and good integrity, which can be used in AAV gene therapy for WD. By means of using an engineered tissue-specific promoter and a truncated natural ATP7B gene having a unique sequence, the sequence length is suitable for packaging into an active adeno-associated virus particle, potential side effects caused by gene expression in tissues other than the liver are minimized, and it is expected to have better in vivo safety.

The invention discloses a structure-driven single cell transcriptomics immune cell annotation system and method, and the system comprises a data loading and preprocessing module which is used for loading a marker table and data after single cell clustering, and extracting the expression characteristic statistic of each cluster from the marker table and data to form a characteristic matrix; the basic structure annotation module is used for carrying out preliminary structure annotation on peripheral blood mononuclear cells and tissue samples; the tissue extension annotation module is used for completing structure extension and refinement under tissue expression drift on the basis of preliminary structure annotation according to a sample structure annotation requirement from peripheral tissues; and the structure dimension auxiliary annotation module is configured to integrate the dimension reduction coordinates and the adjacency graph information after being triggered, and perform spatial consistency verification and auxiliary correction on the low-confidence annotation result. According to the method, an automatic annotation function with high accuracy, strong controllability and good interpretability can be realized, and the method has high interpretability and robustness.

The invention discloses Lap3a, Lap3b and Lap3c genes of bactrocera cucurbitae and an application of the Lap3a, Lap3b and Lap3c genes. The space-time and tissue expression profiles of the Lap3a, Lap3b and Lap3c genes of the bactrocera cucurbitae show that the three genes are specifically and highly expressed in testis of male bactrocera cucurbitae, especially in transformation regions in testis tissues of adult bactrocera cucurbitae. The Lap3a, Lap3b and Lap3c genes play important functions in the process of participating in maintaining the male reproduction of the bactrocera cucurbitae, when the Lap3a, Lap3b or Lap3c genes of male bactrocera cucurbitae are inhibited, the number of male sperms of the bactrocera cucurbitae is reduced, the reproductive power of the male bactrocera cucurbitae is reduced, the treated male bactrocera cucurbitae is mated with normal female bactrocera cucurbitae, and the egg laying amount and egg hatching rate of the female bactrocera cucurbitae are reduced. The three genes are expected to become SIT potential targets to be applied to pest control, and a new thought is provided for green control of bactrocera cucurbitae.

According to the application of the inhibiting substance of the E3 ubiquitin ligase SYVN1 in preparation of the gastric cancer treatment medicine, the expression level of the target SYVN1 targeted by the adopted small interfering RNA in cancer tissue of a gastric cancer patient is higher than that in para-carcinoma tissue, the expression level is in negative correlation with survival time, and the unique clinical application value is achieved.

The invention discloses an adeno-associated virus overexpressing GPR110 and application of the adeno-associated virus in preparation of a medicine for treating metabolism-related steatohepatitis. The adeno-associated virus comprises a GPR110 gene sequence encoded and operably connected to a liver specific promoter TBG, an in-vivo experiment is carried out in a constructed MASH mouse model, and the result shows that the expression of the GPR110 gene in the liver tissue of the MASH mouse model is reduced, so that the GPR110 gene can be used as a biomarker and a therapeutic target of MASH, and the application of the GPR110 gene in the MASH mouse model is proven. According to the present invention, the MASH-related symptoms can be significantly improved through the AAV adeno-associated virus targeting liver specific overexpression GPR110, wherein the MASH-related symptoms comprise liver triglyceride content reducing, liver injury relieving, liver inflammation improving and liver fibrosis improving; the AAV-mediated GPR110 overexpression can effectively improve the MASH pathological process, and new intervention drugs and means can be provided for MASH treatment.

Particularly relates to nucleic acid for coding ATP7B, an adeno-associated virus vector and application of the adeno-associated virus vector in preparation of medicines for preventing and treating hepatolenticular degeneration. The invention provides an ATP7B GOI vector which can be packaged into an adeno-associated virus with cell viability, high yield and good integrity, and can be used for AAV gene therapy of WD. By using an engineered tissue-specific promoter and a truncated natural ATP7B gene with a unique sequence, the sequence length is suitable for being packaged into live adeno-associated virus particles, potential side effects caused by expression of the gene in tissues outside the liver are reduced to the greatest extent, and better in-vivo safety is expected.

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-human prostate specific membrane antigen (PSMA) antibody and application thereof. The amino acid sequences of CDR1-3 on a light chain variable region of the antibody are respectively shown as SEQ ID NO.3-5, and the amino acid sequences of CDR1-3 on a heavy chain variable region of the antibody are respectively shown as SEQ ID NO.8-10. The antibody with the complementarity determining region sequence provided by the invention can specifically recognize PSMA expressed by cells and tissues, has good affinity in combination with the PSMA, has high recognition sensitivity, can effectively resist the interference of complex non-target protein components in the cells / tissues, has no specific combination with non-target antigens, is suitable for immunological detection of the PSMA, and has good application prospects. The kit is especially suitable for western blot detection and immunohistochemical methods, and can reduce the occurrence rate of false positive and false negative results and improve the accuracy of immunodiagnosis.

The invention belongs to the technical field of gene engineering and biology, and relates to screening, construction and application of a modified AAV-8 serotype for gene targeting and expression. According to the modified AAV-8 serotype, ten amino acid sequences are inserted between the 590th amino acid and the 591th amino acid of a capsid protein sequence of the AAV-8 serotype as shown in SEQ ID NO.2, the first, second and tenth amino acids are protected amino acids, and the third to ninth amino acids are amino acid sequences obtained by screening. The modified serotype targeted retina outer nuclear layer and the retina pigment epithelial cell layer have stronger fluorescence effect after infection and can be obviously and intuitively observed, an exogenous gene is efficiently and widely expressed in the targeted tissue retina outer nuclear layer and the retina pigment epithelial cell layer, and under the condition that the same virus amount is used, the expression efficiency is greatly improved. And compared with a wild serotype, the serotype has better and more targeted tissue expression.

Disclosed herein is a method for detecting the expression of xanthine oxidase in polycystic kidney disease, or for delaying or treating the onset of polycystic kidney disease, or both. The inventors have made the remarkable discovery that abnormal purine metabolism is not associated with the disease, but rather that increased tissue expression of xanthine oxidase / xanthine dehydrogenase (XO / XDH) expression and the production of uric acid and oxygen radicals acting alone or in combination may act as primary mediators of the progression of structural and functional dysfunction. A method involving measuring and evaluating tissue XO / XDH expression in polycystic kidney disease or any cystic tissue is specifically exemplified. Moreover, a method involving administering a composition of agents, including urate lowering agents, to a patient susceptible to developing polycystic kidney disease in a regimen that reduces the expression of XO / XDH and reduces the expression of intracellular uric acid or free oxygen radicals in tissue is specifically exemplified.

The application discloses a kind of ZmDAR1b protein in the method for regulating corn height, comprising the following steps: S1 cloning corn gene ZmDAR1b;S2 analysis corn gene ZmDAR1b's tissue expression pattern;S3 create corn ZmDAR1b gene editing knockout mutant.The ZmDAR1b protein described in the application, regulate protein activity or content or regulate ZmDAR1b protein coding gene expression substance, can regulate plant height, stem length, cell expansion, kernel size.The application obtains the transgenic knockout mutant material of ZmDAR1b by gene editing technology and corn genetic transformation technology, and carries out multi-year multi-point field test and observation, and the biological function of ZmDAR1b gene in corn is determined, which provides important gene resources for corn excellent variety breeding, and has very important application value.

The invention relates to the technical field of medical detection. The invention aims to provide a nano-probe for detecting the expression of an epidermal growth factor receptor of head and neck squamous carcinoma cells. The nano-probe is a nano-particle with a core-shell structure, the nano particle takes a nano Au particle as a core, and 4-mercaptobenzoic acid (4-MBA) is adsorbed on the surface of the Au core as a Raman signal internal reference molecule; according to the nanoparticle, SiO2 is used as a shell, and PEG and EGFR antibodies are linked to the SiO2 shell. According to the nanoprobe, head and neck squamous cell cancer cell and tissue imaging is researched by adopting a surface enhanced Raman spectroscopy technology, tumor cell and tissue EGFR expression images are drawn, and quantitative analysis and research are carried out. A rapid, visual and accurate new tool is provided for clinically detecting the squamous cell carcinoma of the head and neck and the EGFR expression condition of the squamous cell carcinoma of the head and neck.

The invention provides a gene with an antiviral effect and application thereof, the nucleotide sequence of an open reading frame of the gene is SEQ ID NO: 2, and the amino acid sequence of a coding protein of the gene is SEQ ID NO: 1. The invention also provides a method for increasing the sensitivity of the beet armyworm to the beet armyworm nuclear polyhedrosis virus, which is used for reducing the expression quantity of the gene in the beet armyworm. According to the invention, a gene with an antiviral effect is screened from an important agricultural pest beet armyworm. The sequence structure and tissue expression mode of the gene are analyzed, incremental expression and RNA interference technologies are utilized to prove that the gene has an anti-SeMNPV effect, a new target is provided for better utilizing baculovirus to prevent and control beet armyworms in production, and the gene has a good application prospect.

The present invention discloses a protein that mediates probiotic colonization and its application in regulating intestinal homeostasis, belonging to the field of disease prevention and control and aquaculture technology. The present invention reports for the first time a protein that mediates probiotic colonization and its encoding gene. Through research, the present invention found that the protein functions in the intestine at the expression level of various tissues of shrimp. The protein has the function of helping probiotics to colonize in the intestine and can increase the survival rate of shrimp under Vibrio infection. It shows excellent disease resistance in shrimp farming, especially in the prevention and control of Vibrio disease. The technology of using the protein to increase the content of probiotics in the intestine of shrimp and then prevent Vibrio infection has the characteristics of being green and environmentally friendly, easy to operate, and long-term effective. It provides an efficient and safe solution for shrimp farming and has broad application prospects and economic benefits.

The invention discloses a DLL1 antibody which comprises three different scFv sequences, and each scFv sequence correspondingly comprises one of a 3P53 sequence, a 3P47 sequence or a 4P108 sequence; 3P53-IgG, 3P47-IgG and 4P108-IgG antibodies having high affinity with human and mouse DLL1 are constructed by using VH and VL sequences derived from 3P53, 3P47 and 4P108 sequences correspondingly contained in different scFv sequences, and the antibodies can recognize human and mouse DLL1 protein molecules, DLL1 expressed by cell membranes and DLL1 expressed by tissues, can be used for ELISA, WB, IHC and other detection methods, and can be used for detecting human and mouse DLL1 protein molecules, cell membrane expression DLL1 and tissue expression DLL1. Therefore, the DLL1 has a better application prospect in the aspect of detecting molecule, cell and tissue levels.

The invention discloses application of an mRNAs marker in early diagnosis of lung signet-ring cell carcinoma and a kit. The marker is a combination of C1QA, CRISPLD2, ROBO4, SPARCL1, SFTPB, LAMC3, FN1, EMP1, CD68, THBS1, PRG4, ICAM1, PODXL, KDM6B, ENPP2, KLF10 and THBD (tetrahydrofuran). According to the invention, FFPE cancer tissue of a patient with primary pulmonary signet ring cell carcinoma diagnosed by an existing detection means in clinic is used as a sample, para-carcinoma tissue is used as a control sample, mRNA expression profiles of the cancer tissue and the control tissue are detected by adopting an RNA sealing technology, and 17 differentially expressed mRNA markers are found by applying statistics and a machine learning method. Based on the 17 found markers, a risk scoring model with high sensitivity and specificity is constructed, and the sensitivity gt of the model to early-stage lung signet-ring cell carcinoma diagnosis; the specificity is gt; 89%.

The invention discloses an mRNAs marker and a kit for early diagnosis of pulmonary signet-ring cell carcinoma. The marker is a combination of FASN, RGPD2, ACSL1, ABCA3, MT2A, ITPKC, FKBP5, CEACAM6, HP, CAPN8, FCGBP and MUC16, and is characterized in that the marker is a combination of FASN, RGPD2, ACSL1, ABCA3, MT2A, ITPKC, FKBP5, CEACAM6, HP, CAPN8 and MUC16. According to the invention, FFPE cancer tissue of a patient with primary pulmonary signet-ring cell carcinoma diagnosed by an existing detection means in clinic is used as a sample, para-carcinoma tissue is used as a control sample, mRNA expression profiles of the cancer tissue and the control tissue are detected by adopting an RNA sealing technology, and 12 differentially expressed mRNA markers are found by applying statistics and machine learning methods. Based on the found 12 markers, a risk scoring model with high sensitivity and specificity is constructed, and the sensitivity gt of the model to early-stage lung signet-ring cell carcinoma diagnosis; the specificity is gt; 60%.