DBR2 gene promoter for specific expression in plant glandular hairs

A plant glandular hair and promoter technology is applied in the field of gene promoters in the field of genetic engineering technology, and can solve problems such as normal growth burden and harm of plants

Inactive Publication Date: 2012-10-31
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This promoter can drive the expression of foreign genes in all tissues and organs, which may cause certain burden and harm to the normal growth of plants

Method used

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  • DBR2 gene promoter for specific expression in plant glandular hairs
  • DBR2 gene promoter for specific expression in plant glandular hairs
  • DBR2 gene promoter for specific expression in plant glandular hairs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0027] Step 1, Cultivate Artemisia annua sterile vaccine

[0028] Artemisia annua seeds were soaked in 75% (v / v) ethanol for 1 min, then soaked in 20% (w / v) NaClO for 20 min, rinsed with sterile water for 3-4 times, blotted the surface moisture with sterile absorbent paper, and inoculated Cultivate on hormone-free MS solid medium at 25°C under 16h / 8h (light / dark) light (14 days) to obtain sterile vaccines of Artemisia annua.

[0029] Step 2: small amount of extraction of total DNA from Artemisia annua leaves by CTAB method

[0030] Take about 100mg of leaves into a 1.5mLEP tube, add 600μL of preheated CTAB buffer containing 2% (v / v) β-mercaptoethanol, grind the leaves with a grinding rod until there are no obvious particles; place the EP tube in The cells were fully lysed in a water bath at 65°C for 45 minutes. After the solution is cooled, add an equal volume (600 μL) of 24:1 (v / v) chloroform:isoamyl alcohol, mix well, let stand for 5 minutes, and centrifuge at 25°C and 13,...

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Abstract

The invention discloses a DBR2 gene promoter in the technical field of gene engineering. The promoter has an SEQ ID NO.1 1520bp nucleotide sequence in a sequence table; and the promoter can guide the gene to specifically express in plant glandular hairs and can be applied to genetic engineering breeding for plant glandular tissue expression utilization and metabolite production. The DBR2 gene promoter can guide the gene to specifically express in plant glandular hairs and is significant to the genetic engineering breeding for plant glandular tissue expression utilization and metabolite production.

Description

technical field [0001] The invention relates to a gene promoter in the technical field of genetic engineering, in particular to a DBR2 gene promoter specifically expressed in plant glandular hairs. Background technique [0002] Artemisia annua L. is an annual herb belonging to the family Asteraceae. Artemisinin is a sesquiterpene lactone compound containing a peroxide bridge structure isolated from its aerial part. It is currently recognized as the most effective drug for treating malaria in the world, especially for cerebral malaria and chloroquine-resistant malaria. efficient. Currently, the most effective treatment for malaria recommended by the World Health Organization is artemisinin combination therapy (ACTs). In addition, with the gradual deepening of pharmacological research on artemisinin, scientists have found that artemisinin and its derivatives also have anti-inflammatory, anti-schistosomiasis, anti-tumor and immune regulation functions. It can be seen that art...

Claims

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Application Information

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IPC IPC(8): C12N15/113A01H5/00
Inventor 唐克轩陈韵斐王博石张凯思
Owner SHANGHAI JIAO TONG UNIV
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