171 results about "Terpene lactones" patented technology

The invention discloses a method for controlling the quality of ginkgo leaves and an extract thereof. After the screening of a great quantity of experiments, the method adopts ultra-high performance liquid chromatography for detection; and the method can be used for detecting three types of components including flavone, terpene lactones and phenolic acid, twenty-four active compounds in total, in the ginkgo leaves and the extract of the ginkgo leaves. The results of the experiments prove that the method is high in detection sensitivity and good in stability, and can be used for objectively, comprehensively and accurately evaluating the quality of ginkgo leaf medicines, the extract of the ginkgo leaves and ginkgo leaf preparations, thus having important significance on controlling the quality and guaranteeing the clinical treatment effect.

The invention discloses a preparation method of a ginkgo leaf biological feed additive. The preparation method comprises the following steps: with the ginkgo leaf as a main solid state fermentation substrate and probiotic bacillus as a strain, performing solid state fermentation under suitable nutrient conditions and culture conditions; and drying and crushing to obtain the biological feed additive. The biological feed additive contains a variety of bioactive substances such as flavonoid compounds and terpene lactone and can improve the immunity and the antioxidant capacity of an animal body; through biological fermentation, the probiotic bacilli are rich in the biological feed additive, so that the biological feed additive can adjust the intestinal flora balance of the animal, improve the intestinal microecological environment, promote the animal growth, reduce the intestinal diseases of the animal, and improve the disease resistance of the animal body; and the biological feed additive can significantly improve the productivity of livestock and poultry, and can substitute or partially substitute antibiotics, thus providing a health guarantee for consumption and use of livestock and poultry food.

The invention discloses pentacyclic triterpene-13,28-lactones with antineoplastic activity, shown as a general formula I, or pharmaceutically acceptable salts thereof as well as a preparation method and medical application thereof, belonging to the field of biological medicines. The preparation method of the compounds, provided by the invention, has the advantages of mild reaction conditions, simplicity in operation, higher yield as well as strong practicability and structural universality. Shown by a pharmacologic test result, the compounds provided by the invention have favorable antineoplastic activity and can be applied as an antineoplastic drug in clinic.

The invention relates to an effective method of extracting puerarin and green food medicines made by it, and the steps include: using alcohol to soak, extracting several times, evaporating the alcohol, converting the extract into water solution, filtering out suspended substances; adopting polymeric absorbent to absorb, concentrating and drying and obtaining Gegen extract, by absorbing and eluting, further making flavone glycoside and terpene inner ester reach the specified indexes. Putting the extracted puerarin in drinks, beers, distilled spirits, teas, milk powders, etc; or adding the puerarin in the tablets, capsules, and electuaries or injectas. The pure plant natural food has proper taste. The Gegen is moderate, peppery and sweet, disperses the internal coldness and relieves the heat of muscle, promotes blood circulation, quenching the thirst and detoxicates alcohol toxicity, etc. and it has good curing effect on high blood pressure, coronary heart disease, heartstroke, dispels the wind, etc.

The present invention belongs to the field of Chinese medicine preparing technology. The ginkgo leaf extract has total flavone and total terpene lactone content over 80 %, total flavone content over 70 %, flavonoid glycoside content over 40 % and total terpene lactone content over 10 %. The extraction process includes the following steps: extracting, purifying and separating, concentration, drying, etc. Compared with available technology, the present invention has the advantages of reasonable technological path, being suitable for industrial production, low production cost, high extracting rate of effective components, etc. The present invention also discloses the infusion liquid and powder for injection with the ginkgo leaf extract and their preparation process. The ginkgo leaf extract has wide application in medicine for treating cardiac vascular diseases.

The invention provides a method for detecting a ginkgo leaf extract and diphyridamole injection preparation. The method comprises the step of fingerprint test and content test of the ginkgo leaf extract and diphyridamole injection preparation, wherein the fingerprint test refers to fingerprint test of flavonoid and terpene lactones in a gingko leaf extract; and the content test refers to test of flavonoid and terpene lactones in the gingko leaf extract. Compared with the prior art, the method can realize more effective product quality control, as well as authentication and content test of effective component monomers, of the ginkgo leaf extract and diphyridamole injection preparation, is very good in precision, stability and repeatability and is another excellent guarantee for the variety curative effect.

The present invention relates to a new-type high-effective safety medicine-ginkgo leaf total lactone for curing depression. It is a terpene lactone extracted from ginkgo biloba. L or other portions of said plant, such as ginkgo nut, ginkgo root bark and ginkgo twing, in which five terpene lactone compounds of bilobalide A, bilobalide B, bilobalide C, bilobalide J and bilobalide are mainly contained.

The invention discloses a separation technique for multiple components in ginkgo leaves. Ginkgo leaves are used as a raw material. By combination of ultrasonic extraction and supercritical CO2 extraction-distillation, separation of multiple effective components is realized. The separation technique provided by the invention comprises the following steps: firstly, extracting the ginkgo leaves by ultrasonication in ethanol solutions with different concentrations for two times, and carrying out the following processes on filter liquor and filter residue obtained by filtration: (1) carrying out supercritical CO2 extraction and separation on the filter residue to obtain polyprenol compounds, and separating raffinate by water extraction and alcohol precipitation to obtain ginkgo polysaccharides; and (2) carrying out ultrafiltration and concentration on the filter liquor, and firstly separating to obtain ginkgolic acids by the supercritical CO2 extraction at a proper temperature and a properpressure; and changing extraction conditions, starting a rectifying column, heating by the temperature variation in the axial direction in four segments, and continuously extracting and separating toobtain ginkgo terpene lactones and flavonoid compounds. The separation technique provided by the invention has the advantages that active ingredients are difficult to decompose or destroy, harmful chemical reagent residues do not exist, the extraction efficiency is high, the separation effect is good and the like, solves the problem that the content of poisonous phenolic acid in ginkgo leaf extract exceeds the standard, and is an environment-friendly, economic and industrialization-suitable technique.

The invention discloses a folium ginkgo and aloe tobacco substitute product and a preparing method thereof, and belongs to the technical field of tobacco substitute products. The fallibility of a tobacco rod is increased through complementation mixing of folium ginkgo and aloe; a cellulose and an activating agent are added in the anaerobic digestion process of the folium ginkgo and the aloe, so that the contents of phenolic acid, cyanide and anthraquinone in the folium ginkgo and aloe tobacco substitute product are effectively reduced, and the contents of effective ingredients including a flavonoid compound and terpene lactones are improved; in the preparing method, through three-stage type initial baking and rebaking in the backing process, the produced tobacco rod is bright, oily and moist; a tobacco extract is sprayed for adjusting the color and increasing the smell of tobaccos, and therefore the two main materials are evenly mixed; a moisture keeping material, a burning adjusting material, a mildew preventive, a seasoner and a perfume increasing material are sprayed, so that the offensive odors of the produced tobacco rod are fewer, thrill is small, and the fragrance is relatively pure; and through compatibility mixing of the product and traditional Chinese medicinal materials, the safety of the folium ginkgo and aloe tobacco substitute product is guaranteed, the taste is moderate, and the better medicine effect is achieved.

The medicated cardiovascular rack carries active component containing ginkgo terpene lactone. After being implanted inside in vivo lumen, the medicated cardiovascular rack can release ginkgo terpene lactone continuously to inhibit the proliferation of smooth muscle cell and the extracellular matrix synthesis. The medicated cardiovascular rack has also functions of promoting endothelium formation, antagonizing thrombosis, antagonizing oxidation, resisting inflammation, etc.

The invention discloses a method for removing ginkgolic acid from a ginkgo biloba extract. The method comprises the following steps of: extracting, performing column chromatography, and drying, wherein the column chromatography process comprises the following steps of: regulating the relative density of a filtrate obtained by extracting and filtering by using water to be 1.05 to 110 to obtain an extracting solution, allowing the extracting solution to pass through a macroporous adsorption resin exchange column in a ratio of adding 1.5 to 3 milliliters of extracting solution into each gram of resin, washing by using the water, eluting by using 60 to 80 mass percent ethanol solution, and collecting 60 to 80 mass percent ethanol eluent, wherein the macroporous adsorption resin is an LSA series macroporous adsorption resin. According to the ginkgo biloba extract obtained by the method, the content of total flavonol glycosides is more than 35 percent, the content of total terpene lactones is more than 12 percent, and the content of total ginkgolic acid is less than 1 ppm.

The invention discloses an anti-aging compound which comprises spirulina polysaccharide and folium ginkgo extract, and the mass ratio of the spirulina polysaccharide to the folium ginkgo extract is 0.35 to 1.5:0.35 to 1.5. The preferred mass ratio of the spirulina polysaccharide to the folium ginkgo extract is 2:1. The spirulina polysaccharide is water-soluble heteropolysaccharide, and the content of the polysaccharide thereof in percentage by mass is more than or equal to 93 percent. The content of general flavone of the folium ginkgo extract in percentage by mass is more than or equal to 24 percent, and the content of terpene lactones thereof in percentage by mass is more than or equal to 6 percent. The anti-aging compound comprises the spirulina polysaccharide and the folium ginkgo extract, can play the cooperative and synergistic roles by combination. because both the spirulina polysaccharide and the folium ginkgo extract contain hydroxyl or phenolic hydroxyl structures, thereby significantly improving the anti-aging effect.

The patent refers to the field of 'treatment of fibres, threads, yarns, fabrics, feathers, or fibrous goods made from such materials'. The invention discloses a nanometer antibiosis cashmere knit fabric which has a moderate price, slippery handfeel and soft gloss and can remove peculiar smell and inhibit the growing of bacteria. The nanometer antibiosis cashmere knit fabric comprises 0.0005 to 0.0025 weight percent of nano silver, 0.01 to 0.1 weight percent of ginkgo leaf extract, and the balance of cashmere, wherein the main antibacterial ingredients of the ginkgo leaf extract comprise ginkgo biloba terpene lactones, ginkgo flavones, and ginkgoic acid. The invention also discloses a production method of the nanometer antibiosis cashmere knit fabric. The method comprises the following steps of: preparing a ginkgo leaf extract/ nano silver composite antibacterial agent; treating the cashmere knit fabric with the ginkgo leaf extract/ nano silver composite antibacterial agent; and then performing conventional process flow of dehydration, soaking in an adhesive, performing dehydration, rolling and washing in a soft way, drying, and ironing to obtain a finished product. The treated cashmere knit fabric has excellent and lasting antibacterial performance, can effectively inhibit the growing of bacteria when clothes are worn, also can remove peculiar smell and reduce worm damage, does not have toxicity or stimulation to skin, and is safe and clean and protects heath.

The invention discloses a preparation method of total terpene lactones with low phenolic acid, which uses ginkgo biloba extract as a raw material, uses ethyl acetate backflow to extract and uses amide resin to purify and deacidify. The preparation method takes the ginkgo biloba extract as the raw material and water saturated ethyl acetate as a solvent; at the weight measurement ratio of extractives to the solvent of 1:1-15, the extractive and solvent are extracted for 0.5-5 hours at the temperature of 20-90 DEG C and filtered; filtrate is concentrated to obtain extractum; after the extractum is adsorbed by polyamide resin, the extractum is eluted by 10-50% of lower alcohol solution; fraction is collected, concerted and dried; and the fraction is smashed to obtain dry powder in which the total terpene lactone content is above 50% and ginkgoic acid is lowered than 1 ppm. The preparation process of the invention has short extract time, low extract temperature, high extract efficiency, small solvent amount, solvent and energy saveing and lowered production cost; the deacidifying process method is convenient to operate and can lower the ginkgolic acid below 1 ppm; and the technical method provided by the invention can effectively remove tannin and pigment in extractives.

A deacidified ginkgo leaf and its extract are disclosed. The deacidified ginkgo leaf contains flavone glycoside and terpene lactone, but almost not contains alkylphenate of original ginkgo leaf. The flavone glycoside and terpene lactone can be extracted by organic solvent or supercritical CO2 method. The deacidified ginkgo leaf can be used to prepare medicine for preventing and curing coronary heart disease, angine pectoris, cerebral thrombus and cerebral disfunction, health-care food and health-care tea.

The invention relates to a water-soluble ginkgo leaf extract and its preparation technology, The method of the invention comprises: taking common ginkgo biloba as a raw material, mixing with acetone and extracting, concentrating, diluting by adding water, cryoprecipitating, filtering, performing a gradient elution to a filtrate through macroporous adsorption resin, carrying out an alcohol precipitation by ethanol with high purity, water extraction and cryoprecipitating, concentrating, drying to obtain the water-soluble ginkgo leaf extract, wherein the content of total flavonol glycoside is greater than or equal to 27%; the content of lactone content is 9%-12%, wherein the content of Terpene Lactones A is greater than or equal to 2.1%, the content of Terpene Lactones B is greater than or equal to 0.9%, the content of Terpene Lactones C is greater than or equal to 1.2%, and the lactone ratio of GA:GB:GC is close to 7:3:4; the water-solubility is greater than and equal to 10%; the ginkgolic acid is less than and equal to 1ppm, which can be taken as a raw material used for injection. The invention has the advantages of simple production technology, high and stable product quality, high finished product yield, low cost and good economic benefit.

The invention discloses a preparation method of a low-acid and high-quality ginkgo leaf extractive, which comprises the steps of extraction, fine purification and separation and concentrated drying. In the ginkgo leaf extractive obtained in the method, the total content of flavones is up to over 65% (wherein a flavonoide content is up to over 35%), the total content of terpene lactone is up to over 10%, the content of 3,3'-dimethoxy-4,4'-dyhydroxy diphenylethene is up to over 0.05%, and the content of ginkgoic acid can be less than 2ppm. Compared with the prior art, in the invention, the content of the 3,3'-dimethoxy-4,4'-dyhydroxy diphenylethene is quantitatively controlled for the first time, the low-acid and high-quality ginkgo leaf extractive has high enrichment and extraction rate ofactive constituents, low content of the ginkgoic acid as a harmful constituent and has the characteristics that a production process meets the requirement of industrial large-scale production, and a process route is more reasonable.

The invention discloses a preparation technology of a low-acidity and high-purity terpene lactone extract in gingko root and bark. The technology comprises the following steps: by taking gingko root and bark as raw materials, crushing into coarse powder; adding high-concentration ethanol to extract, concentrating, adding water to dilute, adsorbing by polyamide adsorption resin and eluting; concentrating an eluate, recrystallizing by ethanol, and separating the obtained coarse crystal by high-performance liquid chromatography, so as to prepare the terpene lactone extract, wherein the total lactone content is greater than or equal to 95%, and the total ginkgo acid content is smaller than or equal to 1ppm. By adopting the method disclosed by the invention, the defects of the existing production technology are overcome, and the method has the characteristics of being stable and controllable in production technology, simple and convenient to operate, high and stable in product quality, high in product yield, low in production cost and the like; the gingko root and bark can be changed into precious resources, so as to fully exert the economical value.

The invention relates to the field of medicine detection, in particular to a high-performance liquid chromatography detecting method for measuring the content of flavonoid compounds and terpene lactone compounds in ginkgo leaves (or ginkgo leaf extract) or preparations of the ginkgo leaves at the same time. The HPLC-DAD-ELSD method is set up, the method can measure the eight flavonoid compounds and three terpene lactone compounds in the ginkgo leaves (or the ginkgo leaf extract) or preparations of the ginkgo leaves at the same time, the measurement result is analyzed, and a new analysis method and a basis are provided for quality control over the ginkgo leaf extract and the preparations of the ginkgo leaf extract.

The invention provides a preparation method of ginkgo dipyridolum injection with high content of ginkgo terpene lactones. The preparation method comprises the following steps: 1), collecting, processing, detecting and classifying ginkgo leaves; 2), extracting and preparing ginkgo terpene lactones extract from ginkgo leaves; 3), extracting ginkgo leaf flavonoid extract; 4), reflowing ginkgo leaf flavonoid extract and partial dipyridamole by adding water, and then filtering so as to be prepared into mixed liquor; 5), mixing the mixed liquor, ginkgo terpene lactones extract, the rest dipyridamole and auxiliary material to be prepared into ginkgo dipyridolum injection as per conventional preparation method; and in the ginkgo dipyridolum injection, the content of the total ginkgo leaf flavonoid and that of the dipyridamole conform to the conventional requirements, and the content of the ginkgo terpene lactones ranges from 0.5 to 1.2 mg/mL.

The invention discloses a method for inducing flavonoids and terpene lactones to accumulate in plant leaves by using methyl jasmonate and application, and belongs to the technical field of biology. The method comprises the following step: when plant leaf cells are completely mature, spraying a 20-80mg/L methyl jasmonate solution onto the plant leaves for 1-2 times, so that a ginkgo biloba extractwith high active ingredient content can be obtained from the treated plant leaves through a conventional method. Compared with the prior art, the method has the advantages of being simple and easy toimplement, low in cost, free of pollution, stable and efficient; the flavonoids and terpene lactones obtained after regulation and control by the method disclosed by the invention are high in content;wherein the contents of quercetin, kaempferol, isorhamnetin, total flavonoids, ginkgo terpene lactone A, ginkgo terpene lactone B and total terpene lactone are respectively increased by 29.84%, 18.08%, 35.94%, 26.24%, 30.36%, 60.65% and 35.41% compared with a control group.

The invention discloses a process for preparing a water-soluble ginkgo biloba extract used for injection, which comprises the following steps: preparing a medicinal gelatin aqueous solution of 0.1-0.3 percent; dissolving the water-soluble ginkgo biloba extract by the gelatin aqueous solution; ultrafiltering and drying the dissolved ginkgo biloba extract in vacuum to prepare total ginkgo terpene lactones and a total ginkgo flavone-glycoides extract; and generally mixing the components according to respective contents so as to prepare a finished product. In the water-soluble ginkgo biloba extract, the content ratio of total flavonol glycosides to the total ginkgo terpene lactones is 4:1, thus the water-soluble ginkgo biloba extract can be used as a water-soluble ginkgo injection raw material, thereby enlarging the range of application and ensuring the safety, the effectiveness, the stability and the controllability of product quality.

The invention discloses a preparation method and product of minimal acid water soluble ginkgo leaf extract. According to the method, the ginkgo leaf is extracted by using water as a solvent, the content of ginkgolic acid is reduced by flocculating settling combined with refrigeration, and gingko flavone and terpene lactone are purified and concentrated by resin column chromatography. The content of ginkgolic acid of the product is less than 0.1ppm, the solubility of the product in cold water is 5g/100ml, and two main problems of high content of ginkgolic acid and poor water solubility of ginkgo leaf extract are solved simultaneously. Compared with the prior art, the extraction and preparation process disclosed by the invention has the advantages of little production investment, simplicityof equipment, flow and operation, low production cost, safety of used reagents, no intervention of toxic organic solvent, stable product quality, strong operability in industrialized production and the like.