Method for controlling quality of ginkgo leaves and extract thereof

A quality control method, ginkgo biloba technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of complex processing, cumbersome pre-processing, and long time consumption, and achieve high detection sensitivity, ensure clinical efficacy, and control quality.

Inactive Publication Date: 2013-06-26
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] The current 2010 edition of "Chinese Pharmacopoeia" stipulates in the quality standard of Ginkgo biloba that: the total flavonol glycosides are controlled by measuring the contents of quercetin, kaempferol and isorhamnetin after acid hydrolysis by HPLC-UV method. Processing is loaded down with trivial details, time-consuming is long, and can not fully reflect flavonoid active ingredient; The terpene lactone component in the ginkgo biloba of prior art is to measure with HPLC-ELSD method, and the preparation of this method need testing solution needs pre-treatment through acidic alumina column, The same process is complicated; and there is no quality control method for ginkgo biloba acids, and the active ingredients of ginkgo biloba cannot be fully detected.

Method used

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  • Method for controlling quality of ginkgo leaves and extract thereof
  • Method for controlling quality of ginkgo leaves and extract thereof
  • Method for controlling quality of ginkgo leaves and extract thereof

Examples

Experimental program
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Embodiment 1

[0034] 1, a kind of quality control method of Ginkgo biloba, it comprises the following steps:

[0035] (1) Preparation of sample solution

[0036] Precisely weigh Ginkgo biloba or Ginkgo biloba extract, add 8 times the concentration of 70% methanol, ultrasonically extract twice, each time for 2 hours, combine the extracts, filter, and take the subsequent filtrate to pass through a 0.22 μm filter membrane to obtain a sample solution;

[0037] (2) Preparation of reference substance solution

[0038] Accurately weighed epigallocatechin, catechin, epicatechin, rutin, isoquercitrin, bilobalide, ginkgolide C, quercetin 3-O-α-L-glucosyl -β-D-1,2-rhamnoside, quercetin, ginkgolide B, ginkgolide A, luteolin, quercetin, apigenin, kaempferol, isorhamnetin, spicardin 24 reference substances of flavonoids, norgingko biflavones, genkwain, ginkgo biflavanones, isoginko biflavanones, golden pine biflavanones, ginkgoic acid, and ginkgoic acid were placed in measuring bottles, and the concent...

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PUM

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Abstract

The invention discloses a method for controlling the quality of ginkgo leaves and an extract thereof. After the screening of a great quantity of experiments, the method adopts ultra-high performance liquid chromatography for detection; and the method can be used for detecting three types of components including flavone, terpene lactones and phenolic acid, twenty-four active compounds in total, in the ginkgo leaves and the extract of the ginkgo leaves. The results of the experiments prove that the method is high in detection sensitivity and good in stability, and can be used for objectively, comprehensively and accurately evaluating the quality of ginkgo leaf medicines, the extract of the ginkgo leaves and ginkgo leaf preparations, thus having important significance on controlling the quality and guaranteeing the clinical treatment effect.

Description

technical field [0001] The invention relates to a quality control method of Chinese medicinal materials, in particular to a quality control method of ginkgo leaves and extracts thereof. Background technique [0002] Ginkgo biloba is the dry leaf of Ginkgo biloba L., which is harvested in autumn when the leaves are still green. Nature and flavor: sweet, bitter, astringent, flat. It has the effects of promoting blood circulation and removing blood stasis, dredging collaterals to relieve pain, astringing the lungs to relieve asthma, and reducing turbidity and fat. [0003] The current 2010 edition of "Chinese Pharmacopoeia" stipulates in the quality standard of Ginkgo biloba that: the total flavonol glycosides are controlled by measuring the contents of quercetin, kaempferol and isorhamnetin after acid hydrolysis by HPLC-UV method. Processing is loaded down with trivial details, time-consuming is long, and can not fully reflect flavonoid active ingredient; The terpene lactone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 段金廒钱大玮尚尔鑫唐于平宿树兰
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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