257 results about "Ginkgolide" patented technology

A process for extracting ginkalide B and biobalide from gingko leaf includes such steps as baking gingko leaves, removing impurities, breaking, extracting in aqueous solution of alcohol, purifying by adsorptive column, extracting gingkalide purifying by alumina column, step crystallizing and recrystallizing.

The invention relates to a composite of active ingredients of Chinese herb medicine used for preventing the damage of cerebral ischemia and relevant diseases, in particular to a preparation which is prepared by the active ingredients of the Chinese herb medicine; the preparation is prepared by the active ingredients of the Chinese herb medicine and the conventional preparation technique which is used by carriers permitted by pharmacy, and comprises the following components with the parts by weight: 2 to 10 parts of ginsenoside Rb, 2 to 10 parts of ginsenoside Rg1, 1 to 5 parts of ginsenoside Rd, 1 to 5 parts of ginsenoside Re, 2 to 10 parts of stilbene glycoside, 1 to 5 parts of ginkgolide and 1 to 5 parts of flavonoid mixture which consists of kaempferol and quercetin with the weight rate to be 1:1. The invention has the advantages that the defects that in the traditional compounded Chinese medicine, the composition is complex, the product quality is not controlled effectively and the curative effect is not stable are overcome, has clear ingredients, definite effect, stable quality and obvious control effect on ischemic stroke, sequela, cerebral arteriosclerosis and vascular dementia, can improve the functions of motor nerve, and learning and memory of the patients, has low toxicity and simple preparation method without obvious side effect and is suitable for industrial mass production.

The present invention discloses new process of extracting and separating high purity bilobalide and ginkgetin. By means of macroporous adsorption resin chromatographic separation technology, the water extract of ginkgo material is eluted in macroporous adsorption resin column successively with distilled water, 5-25 % concentration alcohol solution, 0.01-10 % concentration alkali solution, distilled water and 30-95 % concentration alcohol solution, and the elute of alkali solution and the elute of 30-95 % concentration alcohol solution are collected separately, concentrated and purified to obtain bilobalide or ginkgetin. The process has high efficiency, simple operate, low production cost and environment friendship, and the product has high quality and high yield.

The present invention relates to Ginkgolide derivatives, compositions and extracts comprising one or more Ginkgolides and / or derivatives thereof and methods of use of the compositions to treat neurological disorders and as imaging agents.

The invention discloses a process for extracting ginkgolide, ginkgolide injection and process for preparing same, wherein the extracting process consists of disintegrating the ginkgo leaves, leaching by diluted acetone solution, recovering acetone, removing impurities, extracting and purifying with acetic ether, removing impurities with sodium acetate again, reclaiming acetic ether, recrystallizing in ethanol or methanol, filtering, low temperature drying, solubilizing the bilobalide with hydroxypropyl-beta-cyclodextrin to obtain the bilobalide injection.

The invention relates to application of 10-O-(dimethylaminoethyl) Ginkgolide B in preparing antipyretic, analgesic and anti-inflammatory medicaments. Indicated by tests, the 10-O-(dimethylaminoethyl) Ginkgolide B can inhibit cyclooxygenase-2, has obvious effects of dissociating heat, easing pain and resisting various acute and chronic inflammations and has the advantages of less side effect and low toxicity.

The invention discloses a ginkgolide B raw material, which is characterized in that: the ginkgolide B raw material is made through the following method that: ginkgo leaves or ginkgo leaf extracts are taken and boiled in a hydrochloric acid solution; the boiled liquid undergoes organic extraction after filtration; when the obtained extract is condensed, 100 to 200-mesh silica gel is added in the extract to be mixed and dried; silica gel column chromatography is used to collect eluent rich in ginkgolide B, and the ginkgolide B raw material is obtained through crystallization; and the content of ginkgolide B in the ginkgolide B raw material is more than or equal to 90 percent. The ginkgolide B raw material made through adopting the method has high purity of ginkgolide B, simple preparation technological process, high transfer rate and low cost, and is suitable for industrial production.

The invention discloses a golden fungus gingko yellow wine. Alcoholic strength is 8-45%, general flavone content is more than or equal to 1-5 mg / L, ginkgolide is more than or equal to 1-6 mg / kg, total triterpenoid compound content is 30-800 mg / L, protein is more than or equal to 60 mg / kg, soluble sugar is less than or equal to 2.0%, gingko phenolic acid is less than or equal to 5 ppm, and hydrocyanic acid is less than or equal to 0.1 mu g / L. A double-enzyme method is adopted to hydrolyze gingko to obtain gingko hydrolysate which contains active compounds, such as ginkgo flavone, triterpenoid compound, amino acid, peptide, starch and the like, wherein the content of amino acid and peptide is more than or equal to 1%, and starch content is more than or equal to 2%. After being added with gingko leaf extract, the hydrolysate is converted by golden fungus strains, and rice koji is added to obtain fermentation liquor; the fermentation liquor is filtered and blended to obtain golden fungus gingko yellow wine. The wine contains gingko and golden fungus active ingredients and has the efficacy of preventing diabetes and hyperlipidemia, preventing oxidation and improving immunity of body.

The invention discloses a removal technique of ginkgolic acid in a ginkgo leaf extract, which utilizes ginkgo leaf extract which passes through a macroreticular resinous to be raw material, and the process comprises firstly utilizing polyamide which is pre-processed to be a removing agent of the ginkgolic acid, and then changing superpositions of resin pillar with pre-processing strong alkalinity macroporous ion to remove ginkgolic acid. The process has simple technique, stability and low cost, which can effectively remove ginkgolic acid in ginkgo leaf extract, and can not affect yield rates of ginkgo biloba flavonoids and ginkgolide.

A freeze dried ginkgolide powder as injection is prepared through dissolving the raw mixed ginkgolide in alkali solution, stirring thoroughly, dissolving mannitol in it, filtering, aseptic packing, and freeze drying. Its advantages are simple preparing process, high stability and high water solubility.

The invention discloses low-acid ginkgo flavone and an extraction method thereof. The invention is characterized in that the content of the ginkgo flavonoid glycosides of the low-acid ginkgo flavone is over 27 percent, the content of ginkgolides is over 6.5 percent, and the content of ginkgoic acid is lower than 1PPM. The low-acid ginkgo flavone is extracted according to the following method: crushing folium ginkgo, marinating the crushed folium ginkgo in ethanol; recollecting the ethanol used for marinating the crushed folium ginkgo; removing impurities from the marinated folium ginkgo; adding resin to absorb the ginkgoic acid for the first time; eluting with ethanol so as to obtain an eluting solution; adding resin to absorb the ginkgoic acid for the second time; eluting with ethanol so as to obtain an eluting solution; recollecting the ethanol; obtaining the low-acid ginkgo flavone after the drying. Compared with other extraction methods, the extraction efficiency of the low-acid ginkgo flavone extracted through the adoption of the method is improved by 0.2 percent, the content of the ginkgo flavonoid glycosides is improved by 3 percent, the content of the ginkgolides is improved by 0.5 percent and the content of ginkgoic acid is lower than 1PPM.

A separation and purification process for bilobalide monomers is provided. The column chromatography-recrystallization-supercritical CO2 refinement purification combined process is adopted to prepare high purity bilobalide monomers. The column chromatography adopts positive or reverse fixed packing material. Water and ethyl acetate are used for gradient elution. Supercritical CO2 is used for refinement purification and crystallographic form arrangement. The process conditions are: the pressure is less than or equal to 100Mpa, and the temperature is less than or equal to 150 Celsius degrees. The obtained monomers content amounts to 99.8% at the utmost, and the total yield is greater than or equal to 90%.

The invention discloses a gingkgo active ingredient extraction and separation method and use of extracted active matters in medicines for treating cardiovascular and cerebrovascular diseases. The method comprises the following steps: 1) grinding gingkgo leaves, and extracting gingkgo leaf active ingredients, namely flavone and terpene lactone compounds; 2) removing ginkgolic acids; separating gingkgo flavones from terpene lactones; and 4) purifying the terpene lactones by high performance liquid chromatography. The tests of effects of high-purity gingkgo terpene lactone medicines on the heart function of adult and old rats at organ, body and cell levels prove gingkgo terpene lactones have protective effects on the heart of Sprague Dawley (SD) adult and old rats. The method has the advantage that four terpene lactones with high purity of over 98 percent are prepared by high performance liquid chromatography. The ginkgoic acid content in the gingkgo leaf extract is controlled to be within 1ppm, the separation of flavones from terpene lactones is realized, the content of gingkgo flavones after separation reaches over 55 percent, and the gingkgo terpene lactone content reaches over 25 percent. All of the three indexes reach national standards.

The invention relates to a ginkgolide B injection and a method for making the same. The method comprises the following steps that: ginkgolide B is taken as a raw material and is added in ethanol, glycerol and PEG600, wherein the ratio between the ginkgolide B and the ethanol is between 1:25 and 1:200; the ratio between the ginkgolide B and the PEG600 is between 1:25 and 1:600; and the ratio between the ginkgolide B and the glycerol is between 1:12.5 and 1:50. The ginkgolide B injection has good stability and low cost, and each index of the injection meets the requirements of preparation; moreover, the making process of the injection has the characteristics of quick dissolution of a raw material and simple process, and is suitable for industrialized production.

The invention relates to a quality control method of injection, in particular to a quality control method of ginkgolide injection. The invention provides a method for controlling the quality of the ginkgolide injection by adopting high performance liquid chromatography. The method can control the quality of the ginkgolide injection by adopting one or more of a content determination method, a limittest method of dissociation ginkgolide and a chromatographic fingerprint method. The quality control method provided by the invention has the advantages of having high precision, stability and repeatability, and achieving the aim of effectively controlling the quality of the ginkgolide injection in an all-round way.

The present invention relates to a bilobalide B dripping pills preparation and its preparation method. It is formed from effective component bilobalide B and matrix for making dripping pills. The weight ratio of bilobalide B and matrix is 1:1.5-9.

The invention discloses a compound of ginkgolides K of which a general formula is shown in (I), and a composition is prepared by mixing the ginkgolides K compound and pharmaceutically-acceptable medicine carrier, wherein G is alkaline substances. The invention also discloses a preparation method of the compound and treatment use thereof. In the invention, the ginkgolides K compound changes the physical property of the ginkgolides K, thereby the invention has the advantages of good stability, and the ginkgolides K compound has good water-solubility and higher stability. In the compound, a natural structure of the ginkgolides K can be kept, and when the compound is used as an effective component to enter the human body, the activity of the ginkgolides K can be fully developed to achieve theaim of treating an illness.

The invention discloses a composite capable of preventing bacteria, viruses, oxidation and pigment deposition, which is characterized by being prepared from the following raw materials in proportion by weight: 0.1-10 of epigallocatechin gallate (EGCG), 0-10 of chlorogenic acid, 0-10 of asiaticoside and 0-10 of ginkgolide. The invention also discloses application of the composite in preparing drugs, health-care food and cosmetics, which are capable of preventing bacteria, viruses, oxidation and pigment deposition. The composite of the invention has favorable safety, is suitable for curing respiratory tract infection, condyloma acuminatum, herpes progenitalis, chromatosis and other diseases, simultaneously has anti-oxidation and skin whitening functions, protects cells and DNA from being damaged, can prevent and cure dementia and chloasma, and also can be used for cancer adjuvant therapy.

The invention relates to a ginkgolide injection and a preparation method thereof. The ginkgolide injection contains an active component and adjuvant for injection, wherein the active component is composed of ginkgolide A and ginkgolide B. Experimental studies shows that the preparation containing the combination of ginkgolide A and ginkgolide B has synergic effect and excellent mutual solubility thereof, increases the solubility of ginkgolide B and improves stability at a low temperature. Tests prove that the inventive ginkgolide injection has improved stability at a low temperature, so that the shelf life, safety and effectiveness of the injection in cold areas are improved.

The invention discloses a gingko drop pill for prevention and cure of cardio-cerebrovascular diseases, which mainly solves the problem of slow effect caused by the slow absorption rate of the ginkgo tablet during the disease treatment process. The drop pill comprises the following constituents with parts by weight, ginkgo leaf extract 1-60 parts, red sage root extract 0-60 parts, ground substance 20-80 parts. The content of active bilobanone ingredient in the medicament is 1-60%, the content of bilobalide is 0.5-6%.

The present invention discloses a medicine composition containing rhodiola root extract and ginkgo leaf extract for curing angiocardiopathy and cerebrovascular diseases of cerebral apoplexy, coronary heart disease and angina pectoris, etc. Said medicine composition can be made into oral tablet, capsule, granules and injection preparation.

Synthesis of amide adsorptive resin with weak hydrophobic skeleton is carried out by co-polymerizing methyl acrylate with vinyl benzene to obtain ester-based resin, aminolyzing for ester base, acidylating to obtain amide adsorptive resin with weak hydrophobic skeleton, adsorbing flavone compound from Ginkgo extract onto resin by the resin and hydrogen bond synergistic function, flowing ginkgo-lactone, re-crystallizing and eluting to obtain final product. The purity is above 90%, it's simple and non-toxic, it has friendly environment and can be used to supply experimental samples for development of ginkgo lactone.

The invention provides a ginkgolide osmotic pump tablet and a preparation method thereof. The ginkgolide osmotic pump tablet provided by the invention is a double-layer tablet composed of a drug-containing layer and a push layer, and is coated, wherein the drug-containing layer comprises drug release holes. The ginkgolide osmotic pump tablet uses ginkgolide at effective positions of the ginkgo biloba extract as an active component, and a total amount of ginkgolide A, ginkgolide B, ginkgolide Cand bilobalide is not less than 70 %. According to the invention, by combining a solid dispersion solubilising technique and an osmotic pump tablets control-release technology, the ginkgolide is firstly prepared into the solid dispersion, and then further prepared into the ginkgolide osmotic pump tablet on a basis of improving solubility of poorly soluble components, thereby realizing synchronous slow release of complex components in the ginkgolide, reducing drug administration times and improving patient compliance.

The invention relates to a water-soluble ginkgo leaf extract and its preparation technology, The method of the invention comprises: taking common ginkgo biloba as a raw material, mixing with acetone and extracting, concentrating, diluting by adding water, cryoprecipitating, filtering, performing a gradient elution to a filtrate through macroporous adsorption resin, carrying out an alcohol precipitation by ethanol with high purity, water extraction and cryoprecipitating, concentrating, drying to obtain the water-soluble ginkgo leaf extract, wherein the content of total flavonol glycoside is greater than or equal to 27%; the content of lactone content is 9%-12%, wherein the content of Terpene Lactones A is greater than or equal to 2.1%, the content of Terpene Lactones B is greater than or equal to 0.9%, the content of Terpene Lactones C is greater than or equal to 1.2%, and the lactone ratio of GA:GB:GC is close to 7:3:4; the water-solubility is greater than and equal to 10%; the ginkgolic acid is less than and equal to 1ppm, which can be taken as a raw material used for injection. The invention has the advantages of simple production technology, high and stable product quality, high finished product yield, low cost and good economic benefit.

A separation and purification method of ginkgolide A and B monomers takes ginkgolides with larger than or equal to 95% purity as a starting raw material and comprises steps of separation, purification and drying. The purification adopts recrystallization-supercritical CO2 extraction combination technology; firstly monomer GA and GB obtained by crystallizing separation are respectively recrystallized with absolute ethyl alcohol for at least three times according to the ratio of material to liquid 1:5-80, thus obtaining the monomer GA and GB with larger than or equal to 95% purity; and then the supercritical CO2 extraction method is used for respectively extracting the monomer GA and GB with larger than or equal to 95% purity; wherein the extraction pressure is 10-100Mpa and the extraction temperature is 32-150 DEG C. The extraction is repeated for one time to prepare the monomer GA and GB with larger than or equal to 95% purity. According to the weight of the monomer in the staring material, the yield is larger than or equal to 90%. In the invention, no other organic solvent is touched apart from alcohol and CO2; and the method is a green environmental protection technology, has high yield and is particularly suitable for the manufacture of crude drugs.

A composite medicine for treating cardiovascular and cerebrovascular diseases is proportionally prepared from gingko leaf, or its extract, or its general flavone, or the ginkalide and kakonein.

A fatty emulsion of ginkalide B and its preparing process are disclosed. It has the strong antagonism to platelet activating factor.

The invention aims to industrially produce ginkgolide B liposome medicinal composition injection and ginkgolide B liposome medicinal composition oral preparations through uniform formula, process and equipment by a molecule dispersing method. The invention provides a molar ratio formula of the component raw materials for preparing the ginkgolide B liposome medicinal composition, steps and method for preparing ginkgolide B liposome medicinal composition freeze-dried injection and oral preparations, and embodiments for preparation. The ginkgolide B liposome medicinal composition is used for treating cerebral and vascular diseases.