321 results about "Spinal cord lesion" patented technology

The invention discloses a patient improvement effect analysis method for controlling spinal cord electrical stimulation through an implantable brain-computer interface, and relates to the technical field of medical rehabilitation, and the method comprises the steps: multi-dimensional collaborative data collection: implanting electrodes in a target brain region and below a spinal cord injury segment, installing a detection element at an exoskeleton key part, and carrying out multi-dimensional collaborative data collection; a sensor is attached to a lower limb preset muscle group, electroencephalogram signals, SCS stimulation parameters, EXS motion data and neuromuscular response data are synchronously collected, and time correlation marks are embedded; according to the method, the reliability of motion intention decoding is remarkably improved by adopting a mode of combining multi-source signal preprocessing and a multi-mode intention recognition model, and in the signal preprocessing stage, the self-adaptive filtering algorithm combining Kalman filtering and wavelet threshold denoising is applied, so that the motion intention decoding efficiency is improved. SCS electrical stimulation interference, EXS motor noise and physiological noise in the electroencephalogram signals are effectively removed.

The invention discloses a spinal cord injury analysis method and device based on deep learning. The method comprises the following steps: acquiring processed medical image information of a spinal cord part and spinal cord injury physiological information; performing space-time alignment on the medical image of the spinal cord part and the spinal cord injury physiological information so as to obtain an image sequence and an index time sequence after space-time alignment; establishing space-time diagram features according to the image sequence and the index time sequence; splicing the space-time diagram features, the medical image information of the spinal cord part and the physiological information of the spinal cord injury to generate joint features; generating a cross-modal fusion feature; acquiring a trained spinal cord injury analysis model; and inputting the cross-modal fusion features into the trained spinal cord injury analysis model to obtain an injury result. MRI images and physiological indexes are synchronously collected, the limitation of traditional single-mode analysis is broken through, and dual-dimension information of an anatomical structure and functional metabolism is provided for spinal cord injury assessment.

The invention discloses a spinal cord injury state evaluation system fusing pathological data and clinical characteristics, and the system comprises a multi-modal data synchronous collection module which is used for multi-modal original data flow; the damage feature collaborative extraction module is used for extracting structural damage features and functional damage features through a dual-channel deep learning network based on an attention mechanism, and calculating an association weight matrix; the individualized state quantitative evaluation module is used for generating damage state vectors based on weight matrix fusion features and outputting a comprehensive evaluation report through a pre-training model; and the self-adaptive rehabilitation strategy generation module is used for generating a personalized staged rehabilitation treatment scheme according to the comprehensive evaluation report and decomposing the personalized staged rehabilitation treatment scheme into an executable instruction sequence. The method has the following advantages and effects: accurate evaluation of the spinal cord injury state and accurate formulation of the rehabilitation strategy are realized by quantifying the personalized mapping relationship between the spinal cord structural injury and the body functional disorder.

The invention relates to the field of medical dynamic monitoring, in particular to a dynamic monitoring system for a spinal cord injury reconstruction process. Comprising an acquisition and fusion module, a dynamic modeling module, a function evaluation module and an adaptation control module. According to the system, nerve and muscle electrophysiological signals are collected through a wearable surface electrode and a flexible microelectrode array, timing sequence errors are corrected in combination with a calibration sample, and multi-modal signal fusion is achieved. And constructing a topological sequence and time sequence dependence structure, and extracting an incubation period, an activation gradient and response dispersion by adopting a time sequence convolutional network. And identifying function transition and abnormal compensation behaviors through a function evaluation module, and outputting a neural pathway response sequence diagram. And the sampling frequency, the feature weight and the evaluation period are dynamically adjusted based on a Bayesian optimization strategy, and self-adaptive optimization of the monitoring strategy is realized. Therefore, precise modeling and efficient tracking of the neural pathway function reconstruction process are realized.

The invention relates to the technical field of rehabilitation medical treatment and neural engineering, and particularly discloses an epidural electrical stimulation lower limb rehabilitation system and method based on a brain-computer interface, the epidural electrical stimulation lower limb rehabilitation system comprises a weight reduction suspension device, an epidural electrical stimulation module and a brain-computer interface module, the epidural electrical stimulation module controls left and right electrical stimulation through a program controller, and the brain-computer interface module comprises a wireless electroencephalogram acquisition device and an interaction terminal and is used for acquiring electroencephalogram signals of a movement area of a patient and presenting a virtual gait scene and voice prompt at a computer end. The method comprises the steps of electroencephalogram signal collection and feature extraction, motion intention judgment, stimulation control and feedback backtracking, and by combining a motion intention decoding result with electrical stimulation output, activation of a lower limb target muscle group is achieved, a training closed loop is constructed, and the method is used for postoperative rehabilitation of a spinal cord injury patient.

The invention relates to the technical field of spinal cord injury repair, and discloses a multi-mode nerve regulation electric acupuncture control system for spinal cord injury repair, a patient evaluation module is used for establishing a personalized spinal cord injury model of a patient and evaluating the current nerve function state and rehabilitation potential of the patient to obtain a nerve function evaluation result; the parameter setting module is used for setting multi-modal stimulation parameters in electroacupuncture treatment; the signal processing module is used for collecting local field potential of a spinal cord injury area and electromyographic signals around the injury in real time, preprocessing the collected signals and extracting time-frequency characteristics of the signals. The evaluation module is used for inputting the time-frequency characteristics of the signals into an electro-acupuncture evaluation model, evaluating the neural pathway in real time through the electro-acupuncture evaluation model and outputting an evaluation result; the adjusting module is used for adjusting the frequency, waveform and current intensity parameters of the electroacupuncture needle based on the evaluation result and a Q-learning algorithm, searching an optimal electroacupuncture needle parameter combination and feeding back the optimal electroacupuncture needle parameter combination to the electroacupuncture needle; according to the invention, the pertinence and effectiveness of treatment are improved.

The invention discloses application of a cell surface adhesion molecule CD22 as a target spot in preparation of drugs for treating spinal cord injury related diseases. The change of gene expression in the glial scar formation process is represented by space transcriptome sequencing, and the specific expression of CD22 in the glial scar region is up-regulated. According to single cell sequencing, in-situ hybridization and immunohistochemistry, specific high expression CD22 of part of microglial cells in a glial scar area is found. CD22 is knocked out through a genetic means, and it is found that formation of glial scars after spinal cord injury is remarkably reduced through inhibition of CD22. Behavioral detection finds that the error rate of irregular horizontal ladders is remarkably reduced by inhibiting CD22, and fine movement recovery of hind limbs of mice after spinal cord injury is promoted. The siRNA specifically targeting CD22 is injected into the sheath, so that the expression of microglial cells CD22 is inhibited, the error rate of irregular horizontal ladders of hind limbs can be reduced, and the recovery of the fine movement function of the hind limbs of the mouse after spinal cord injury is promoted. The invention provides a new possibility for development of drugs for spinal cord injury and treatment of spinal cord injury.

The invention relates to the technical field of biological medicine, in particular to a functionalized targeted liposome and application thereof, and the functionalized targeted liposome comprises lipid and a targeted ligand, wherein the targeting ligand can be specifically combined with a TREM2 receptor. According to the lipidosome, the targeting ligand can be specifically combined with the TREM2 receptor, accurate targeting of cells with the surface containing the TREM2 receptor is achieved, it is ensured that the cells directly act on target cells, the accuracy of a delivered substance is further improved, and the lipidosome has the advantages of being targeting, high in stability, small in side effect and high in bioavailability. The functionalized targeted liposome can be applied to the development of drug carriers, pharmaceutical compositions and the like, and has wide application prospects, for example, the liposome is dynamically connected with hydrogel molecules through a borate bond to prepare a hydrogel pharmaceutical composition containing the functionalized targeted liposome, and the application prospect is wide. The liposome disclosed by the invention can respond to an inflammatory environment according to pH and ROS changes of a surrounding environment to release the liposome disclosed by the invention, targets cells with TREM2 receptors on the surfaces, namely microglial cells (with the TREM2 receptors on the surfaces) by virtue of the target ligands exposed on the surfaces, regulates the polarization state of the cells, inhibits the polarization of the cells to a pro-inflammatory M1 type and promotes the polarization of the cells to an anti-inflammatory M2 type, so that the anti-inflammatory effect of the liposome is improved. Therefore, the local microenvironment of the spinal cord injury part is improved, and nerve regeneration is promoted.

The invention discloses a progressive dynamic pressure gradient bladder function regulation and control system and method, and aims to solve the problems of high infection rate, bladder injury risk, complicated operation, lack of dynamic pressure regulation and control and the like in the existing bladder management technology. According to the system, the urinary bladder internal pressure, the urine flow rate and the residual urine volume are monitored in real time through the urodynamic monitor, and the pressure gradient is dynamically adjusted according to the health condition of a patient. Progressive pressure training is adopted in the system, the bladder function is gradually enhanced, meanwhile, an emergency adjustment strategy is introduced, and the safety of a patient is ensured. In addition, the system also integrates machine learning and reinforcement learning algorithms, and realizes personalized treatment according to the treatment history and real-time feedback optimization adjustment strategy of the patient. The system has the advantages of precise regulation and control, personalized treatment, improvement of the comfort level of the patient and the like, and the bladder rehabilitation effect of the spinal cord injury patient is remarkably improved.

The invention discloses a magnesium-containing nanoparticle modified biological scaffold composite material as well as a preparation method and application thereof. The magnesium-containing nano particle modified biological scaffold composite material comprises a biological scaffold material and magnesium-containing nano particles modified on the biological scaffold material, the magnesium-containing nano particles comprise nano magnesium oxide particles and / or nano magnesium oxide-polyphenol compound particles, the nano magnesium oxide-polyphenol compound particles are of a shell-core structure, and the shell-core structure is of a hollow structure. Comprising magnesium oxide serving as a core and a polyphenol compound shell layer coating the surface of the magnesium oxide. The magnesium-containing nanoparticle-modified biological scaffold composite material provided by the invention can prolong the degradation and release of magnesium ions, promote improvement of microenvironment problems such as inflammation and active oxygen outbreak after spinal cord injury, regulate and control oxidative stress and inflammatory response, promote nerve regeneration and motor function recovery, and improve the survival rate of spinal cord injury. The problem that nanoparticles stop in a spinal cord injury area is effectively solved, and support is provided for migration, growth and differentiation of endogenous neural stem cells.

The invention provides a culture method, application and detection method of mesenchymal stem cells for treating nervous system diseases, and belongs to the technical field of biological medicines. The invention provides a culture medium of mesenchymal stem cells, a method for culturing and subculturing the mesenchymal stem cells by using the culture medium, and a method for detecting the biological efficacy of the mesenchymal stem cells for treating nervous system diseases. The mesenchymal stem cell preparation disclosed by the invention has a relatively good treatment effect on nervous system diseases, and can be used for improving the new object recognition capability and the cerebral infarction range, inhibiting the release of inflammatory factors, reducing the oxidative stress level and narrowing the spinal cord lesion range so as to improve nervous system injury.

The invention provides application of ACT001 in preparation of a medicine for treating spinal cord injury, and relates to the technical field of biomedicine. The parthenolide structure-improved compound ACT001 disclosed by the invention, as a novel neuroprotective drug, has a remarkable effect, and can be used for practically relieving inflammatory response after spinal cord injury, inhibiting formation of glial scars after injury and protecting neurons so as to promote functional recovery. The medicine can improve the lower limb function recovery of the SCI patient, improve the life quality of the patient, and bring a brand new treatment choice for acute and chronic spinal cord injury patients.

The present invention discloses an FES cycling control method, system, electronic device, and computer program product, relating to the technical field of rehabilitation training methods. The FES cycling control method combines a flywheel, an electromagnetic clutch, and a bicycle crank to provide an FES cycling control method for the quadriceps femoris of patients with spinal cord injury. This method addresses technical difficulties in FES-based fixed cycling rehabilitation exercises, such as the limited torque of electrically stimulated muscles, the difficulty of precise control of muscles as nonlinear elements, and the tendency for long-term continuous electrical stimulation to induce muscle fatigue. The practical effects of FES-based fixed cycling rehabilitation exercises still need to be improved. Compared with traditional electrical stimulation treatment methods, the present invention effectively reduces the intensity of electrical stimulation, improves energy utilization, and achieves energy-saving effects while ensuring rehabilitation needs.

The invention provides a nerve regulation and control system and method for a spinal cord ventral epidural region, and the system employs a microprocessor to generate multiple paths of pulse width modulation signals, and drives a voltage-controlled current source after amplitude adjustment. Stimulation current with adjustable frequency, pulse width and amplitude is output to act on a spinal cord ventral nerve structure. Real-time monitoring of stimulation current of each channel is realized through combination of voltage sampling and a single-pole multi-throw analog switch, feedback information of a body position, myoelectricity, pressure and other multi-mode sensors is fused, a closed-loop control mechanism is constructed, and dynamic adjustment and individualized optimization of electrical stimulation parameters are realized. The system has the advantages of being more accurate in electrical stimulation positioning, more sensitive in response, more intelligent in control and the like, and is suitable for intelligent rehabilitation intervention in scenes of spinal cord injury, cerebral palsy, neurological dysfunction and the like.

The invention discloses a functional hydrogel microsphere and a preparation method and application thereof, and relates to the technical field of medicines.The preparation method comprises the steps that a hyaluronic acid methacrylic acid precursor solution and an IL-1beta shRNA adenovirus stock solution are mixed to obtain an Ad-atHAMA precursor solution; preparing the Ad (at) HAMA precursor solution through an oil-in-water micro-fluidic technology to obtain an Ad (at) HAMA microsphere; and sequentially adding the Ad (at) HAMA microspheres, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide into a 2-morpholinoethanesulfonic acid buffer solution, carrying out activating treatment, adding E selectin, and incubating to obtain the functionalized hydrogel microspheres. The functionalized hydrogel microspheres can inhibit a related mechanism of acute spinal cord injury pathological progress and promote neural function repair by targeting adsorption of neutrophil to regulate Nets-triggered innate immune inflammation cascade.

A system and method is disclosed for measuring muscle reflexes (e.g., a bulbocavernosus reflex) as a tool for identifying / diagnosing dysfunctions (e.g., spinal cord abnormalities, bladder voiding dysfunction, and sexual organ dysfunction) non-invasively by using mechanical stimulation. The system and method includes a probe having a predetermined patient contacting portion, wherein when the contacting portion is moved into contact with a particular area of the patient (e.g., the patient's genitals), the contact induces a muscle reflex. The probe detects the pressure resulting from the contacting portion being abruptly and forcibly brought into contact with the particular area. Such detection is used to electronically initiate capture of electrical responses from a plurality of electrodes placed on the patient's skin in proximity to the particular area. Such electrical responses are processed to determine characteristics of the patient's reflexes of one or more muscles adjacent to the electrodes.

The invention discloses a fusion polypeptide TGNL and application of neural stem cells modified by the fusion polypeptide TGNL in spinal cord injury repair. The fusion polypeptide TGNL is composed of a TAT penetrating domain, a GSTP targeting domain, an NICDi inhibition domain, an MMP-2 activation domain and a flexible Linker connecting the three domains, and the full-length amino acid sequence is as shown in SEQ ID NO. 1. The invention also provides a preparation method of the polypeptide, a neural stem cell (hNSCs-TGNL) for expressing the polypeptide and a construction method of the neural stem cell (hNSCs-TGNL). Experiments show that the fusion polypeptide TGNL can efficiently penetrate nerve cells, specifically target glial scars, is selectively activated by MMP-2, and promotes neural stem cells to differentiate into neurons by inhibiting Notch signals; the hNSCs-TGNL has high survival rate and strong penetrability in an in-vitro glial scar model, and can significantly promote motor function recovery, reduce injury volume, increase axon density and reduce glial scars in a rat spinal cord contusion model. The invention provides a novel candidate drug and a cell treatment strategy for clinical treatment of spinal cord injury, and has remarkable clinical application value.

The invention discloses engineered small extracellular vesicle hydrogel as well as a preparation method and application thereof. The engineered small extracellular vesicle hydrogel comprises a hydrogel matrix, and GDNF-sEVs and a polyphenol compound which are loaded in the hydrogel matrix, wherein the hydrogel matrix comprises a polyacrylamide polymer, xanthan gum and carbomer, the polyacrylamide polymer comprises the following structural unit: [-CH2CH (-CONH-RX-CONH2)-], RX is C1-4 alkylene, and the polyacrylamide polymer comprises the following structural unit:-CH2CH (-CONH-RX-CONH2)-[-CH2CH (-CONH-RX-CONH2)-]. The GDNF-sEVs are small extracellular vesicles loaded with glial cell-derived neurotrophic factors (GDNF-sEVs). According to the engineered small extracellular vesicle hydrogel, through the synergistic effect of the small extracellular vesicles, the glial cell-derived neurotrophic factors and the hydrogel matrix, the injury microenvironment after spinal cord injury can be regulated and controlled, and repair of spinal cord injury is promoted.

The invention relates to the technical field of hydrogel preparation, and particularly discloses injectable self-healing conductive hydrogel with immunoregulation performance, and a preparation method of the hydrogel comprises the following steps: S1, preparing oxidized hyaluronic acid; s2, obtaining a polydopamine nano particle; s3, the polypyrrole nano particles are obtained; s4, preparing two kinds of dispersion liquid and two kinds of premixed solution; s5, adding the two dispersion solutions into oxidized hyaluronic acid, fully stirring to obtain a solution A, adding the two premixed solutions into carboxymethyl chitosan, mixing to obtain a solution B, mixing and fully stirring the solution A and the solution B, and performing Schiff base reaction and self-assembly to form the injectable self-healing conductive hydrogel with the immunoregulation performance. The injectable self-healing conductive hydrogel prepared in the invention has good mechanical properties, can effectively bear stress in application, can effectively control inflammation in the early stage of spinal cord injury, has a good electrical microenvironment, and also has excellent biodegradability.

The invention discloses a medical image-based spinal cord injury analysis method and system, and belongs to the technical field of image analysis, and the method comprises the steps: obtaining MRI images of a target object at a plurality of time points, carrying out the registration and normalization processing, and constructing a time sequence image set; extracting a spinal cord and an injury area by adopting a dynamic segmentation network guided by a spinal cord structure; constructing a time sequence deformation path of the damaged area based on a form tracking algorithm; extracting high-order morphological characteristics including a curvature change rate, an edge contour jump value and a spinal cord compression rate; performing double-domain modeling on the features and neural function scores, and training a structure-function coupling model; the model is used for performing functional state prediction, risk area identification and rehabilitation suggestion output on a new patient, and the method has the advantages of being high in predictability, high in accuracy, remarkable in clinical auxiliary value and the like.

The invention discloses a preparation method and application of oxidative stress microenvironment response intelligent semi-conversion hydrogel, and belongs to the technical field of biological medicine. According to the preparation method disclosed by the invention, a therapeutic drug can be wrapped in the drug and placed in a local injury part, and a large amount of ROS released by oxidative stress in the early stage of spinal cord injury (SCI) is responded, so that conversion from a gel state to a semi-gel sol state is realized, and explosive release of the drug is caused while the ROS is consumed; the medicine treatment concentration is quickly reached in the acute stage of SCI; after the SCI oxidative stress period, ROS release is reduced, and under the action of in-vivo reducing substances, the hydrogel can be recovered from a semi-sol-gel state to a gel state, so that long-term slow release of to-be-released drugs is realized, and later-stage neural restoration of SCI is promoted; through the conversion from the gel state to the semi-sol gel state to the gel state, intelligent drug controlled release responding to oxidative stress can be achieved, and therefore the optimal SCI treatment effect is achieved.

The invention discloses a bionic bacterial outer membrane nano-particle for drug delivery. The bionic bacterial outer membrane nano-particle is characterized by comprising low-toxicity outer membrane vesicles extracted from escherichia coli with msbB genes knocked out, and low-toxicity outer membrane vesicles extracted from escherichia coli with msbB genes knocked out; the medicine carrying liposome is prepared from phosphatidylcholine, cholesterol and DSPE-PEG2000 (Distearoyl Phosphatidylcholine-Polyethylene Glycol 2000) according to a preset mass ratio; the low-toxicity outer membrane vesicles and the drug-loaded liposome are subjected to ultrasonic assistance and 100nm filter membrane extrusion to obtain bionic bacterial outer membrane nanoparticles, the average particle size of the bionic bacterial outer membrane nanoparticles is about 100nm, and the encapsulation efficiency exceeds 85%. The BM-NPs system disclosed by the invention can load small molecule compounds, proteins, nucleic acids and gene therapy vectors, and is suitable for various central nervous system diseases such as spinal cord injury, cerebral apoplexy, traumatic brain injury, ischemia-reperfusion injury and the like.

The invention relates to application of epalrestat in preparation of a medicine for treating spinal cord injury, and belongs to the technical field of biological medicine. The invention provides a novel pharmaceutical application of epalrestat, namely an application of epalrestat in preparation of a medicine for treating spinal cord injury. It is found for the first time that epalrestat can reduce the pyroptosis level of neurons after spinal cord injury by inhibiting an aldose reductase mediated NOX4 / ROCK / Poldip2 oxidative stress pathway, and it is proposed that epalrestat is used for preparing the medicine for treating spinal cord injury. Animal model experiments prove that after epalrestat gavage treatment, AR expression of a mouse spinal cord injury area and pyroptosis of neurons are remarkably reduced, and the motor function and nerve injury of a mouse are remarkably improved. The application of the epalrestat-containing medicine in clinical treatment of spinal cord injury is expected to provide benefits and help for improvement of prognosis of patients.

The invention relates to the technical field of curative effect prediction, in particular to a curative effect prediction method and system for treating spinal cord injury by stem cells, and the method comprises the following steps: obtaining a multi-parameter metabolism sequence and constructing an activation rhythm map, recognizing a neural restoration synchronization section, extracting a metabolism dominant path and evaluating electrical signal response, generating a para-position map layer and predicting a curative effect trend. According to the method, through introduction of cross-time-sequence metabolism and trend synchronous identification of electrophysiological indexes, dynamic mapping of stem cell activation rhythms is realized, neural axon morphological changes and cerebrospinal fluid metabolism parameters are linked, and distribution positions of key response points in a treatment cycle are determined; the method comprises the following steps: establishing a causal path based on a sequence of metabolic mutation and neural restoration events, extracting an evolution stage dominated by a metabolic driving effect, marking a matching trend of a curative effect response and an intervention path on a time axis, identifying the number and positions of trend convergence, withdrawal and interruption areas, and realizing collaborative prediction of a restoration progress and a curative effect trend.

This invention relates to diselenylene-bridged mesoporous silica nanoparticles loaded with baicalin and their applications. The invention constructs a ROS-responsive, microglia-inspired Ba@Se-MSN&BV2 nanoplatform targeting the secondary phase of spinal cord injury. The BV2 cell membrane-mediated enrichment at the injury site, combined with the diselenylene-triggered, on-demand release of baicalin, enables targeted intervention in a highly oxidative and pro-inflammatory microenvironment. In vitro and in vivo studies show that Ba@Se-MSN&BV2 can alleviate oxidative stress and mitochondrial dysfunction, inhibit apoptosis, promote axonal and neuronal survival, and improve motor circuit function. This invention provides a new therapeutic target and research direction for neuroinflammatory injuries driven by redox homeostasis disruption, with significant clinical application prospects and social value.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

Disclosed herein are anti-RGMa antibodies and methods of using these antibodies to treat spinal cord injury, including promoting axonal regeneration, functional recovery, or both and to treat pain, including neuropathic pain arising from spinal cord injury.

The utility model relates to the field of medical instruments, and discloses a working cannula for cervical endoscopic surgery, which comprises a cannula main body provided with a through hole, a first opening of the cannula main body is obliquely arranged, one side, close to a second opening of the cannula main body, of the first opening is defined as a first side, and one side, far away from the second opening, of the first opening is defined as a second side; the sleeve main body is provided with a limiting thread above the first opening, and the limiting thread is matched with the limiting ring to adjust and control the working depth of the sleeve main body; the limiting threads are arranged and matched with the limiting ring, so that the movement of the cannula body can be well limited, the depth of the cannula placed in the intervertebral space is controlled and stabilized during operation, the situation that the cannula body is too deep to penetrate the rear wall of the intervertebral disc and damage the spinal cord is avoided, the operation risk is reduced, and related complications of spinal cord injury are reduced.

The invention belongs to the technical field of biological medicine, and particularly relates to a single-domain antibody VHH-L1 targeting LINGO-1 protein, and the amino acid sequence of the single-domain antibody VHH-L1 is shown as SEQ ID NO: 1. The single-domain antibody has nanomole-level high affinity and excellent specificity, can effectively block the interaction between LINGO-1 and ligands thereof, and remarkably promotes differentiation and myelination of oligodendroglia cells. The invention further provides a neural stem cell subjected to genetic engineering modification, and the neural stem cell can stably and continuously secrete the single-domain antibody VHH-L1. In a spinal cord injury animal model, the engineered stem cell shows an excellent treatment effect, can significantly promote motor function recovery, axon regeneration and myelin sheath repair, and effectively inhibits glial scar formation. The double advantages of cell therapy and long-acting protein delivery are fused, and a brand new efficient treatment strategy is provided for demyelination diseases such as multiple sclerosis and spinal cord injury.

The invention relates to nervonic acid-loaded near-infrared light response type injectable hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological engineering. According to the invention, P (NIPAM-co-NHMAM) and a modified natural polymer material are combined to prepare a temperature-responsive hydrogel capable of realizing reversible volume shrinkage / expansion at a specific temperature, near-infrared light physiotherapy and irradiation are combined to damage local skin, and the hydrogel is stimulated to realize reversible volume deformation by controlling tissue temperature, so that the temperature-responsive hydrogel can be used for preparing the hydrogel with the reversible volume shrinkage / expansion at the specific temperature. Therefore, accurate and wireless space-time regulation and control of nervonic acid release behaviors are realized. Therefore, the nervonic acid loaded near-infrared light response type injectable hydrogel disclosed by the invention can realize efficient carrying and light response controllable release of nervonic acid, promotes functional recovery after spinal cord injury, and has a relatively good application prospect and research significance.