38 results about "Spinal cord lesion" patented technology

This invention relates to diselenylene-bridged mesoporous silica nanoparticles loaded with baicalin and their applications. The invention constructs a ROS-responsive, microglia-inspired Ba@Se-MSN&BV2 nanoplatform targeting the secondary phase of spinal cord injury. The BV2 cell membrane-mediated enrichment at the injury site, combined with the diselenylene-triggered, on-demand release of baicalin, enables targeted intervention in a highly oxidative and pro-inflammatory microenvironment. In vitro and in vivo studies show that Ba@Se-MSN&BV2 can alleviate oxidative stress and mitochondrial dysfunction, inhibit apoptosis, promote axonal and neuronal survival, and improve motor circuit function. This invention provides a new therapeutic target and research direction for neuroinflammatory injuries driven by redox homeostasis disruption, with significant clinical application prospects and social value.

The application belongs to the field of biomedical materials and biomedical engineering technology, and discloses a pH-responsive curcumin sustained-release microsphere hydrogel, a preparation method and application thereof in spinal cord injury repair materials. The preparation method is as follows: short fibers are prepared by pretreating and grinding discarded eggshell membranes; after sodium alginate is dissolved, the sodium alginate is blended with the eggshell membrane short fibers, curcumin is added, and stirring is uniformly performed to form a mixed solution; the mixed solution is injected into a calcium chloride solution by using a drip crosslinking method to form, and an eggshell membrane and sodium alginate curcumin microsphere hydrogel is obtained. The hydrogel has excellent water content, swelling performance, biocompatibility, blood compatibility and drug sustained-release function, and can be applied to spinal cord injury repair.

This invention relates to the field of biomedical materials technology, specifically disclosing a hydrogel material for spinal cord nerve repair containing magnetic nanospheres and its preparation method. The hydrogel material provided by this invention is a photocurable hydrogel formulated using chitosan, dimethylaminoethyl methacrylate, methacryloyloxyethyltrimethylammonium chloride, and magnetic iron oxide nanospheres. Compared with traditional spinal cord nerve repair materials, the hydrogel material provided by this invention has good biocompatibility, is safe and non-toxic, and has a simple and easy preparation method. Photocuring is performed when the hydrogel is injected into the spinal cord injury site, while a magnetic field is simultaneously applied to the injury site. This causes the magnetic nanospheres to rotate according to the magnetic field, forming a series of magnetic channels. As the hydrogel gradually solidifies, the magnetic channels are permanently retained. During the spinal cord nerve repair process, the magnetic channels guide nerve growth, preventing incorrect nerve connections and ensuring the effectiveness of nerve repair.

The disclosure relates to antibodies and antibody-drug conjugates (ADCs) with blood-brain barrier (BBB) and / or cellular penetration and their use in methods of treating neurological disorders, neurodegenerative diseases, and traumatic brain or spinal injuries in a subject.

The application belongs to the technical field of animal experiment instruments, and discloses a pneumatic impact type spinal cord injury animal model preparation device, which comprises an operation table; a fixing unit is arranged on the operation table, the fixing unit is used for fixing an experimental animal and enabling a spinal cord region of the experimental animal to be on the outside; a gas outlet head is arranged vertically downward on the upper side of the operation table, and the spinal cord of the experimental animal is located directly below the gas outlet head; a pneumatic generating unit is connected to the upper end of the gas outlet head, the pneumatic generating unit is used for generating compressed gas, and the compressed gas is discharged downward through the gas outlet head to form a gas column, and the exposed spinal cord region is subjected to non-contact pneumatic impact through the gas column; a lifting driving part is arranged on the upper side of the operation table, an output end of the lifting driving part is connected with the pneumatic generating unit, and the lifting driving part is used for driving the pneumatic generating unit and the gas outlet head to move up and down; the problems that the heavy object falling method in the prior art causes additional tissue damage due to mechanical contact and the standardization degree of the model is low are effectively solved.

This invention relates to the field of natural language processing technology, specifically to a triage method and system for neurological diseases, comprising the following steps: segmenting the syntactic structure of the patient's chief complaint text sequence and identifying nodes; calculating and quantifying the nesting degree of the patient's linguistic organization logic; and obtaining a syntactic potential depth value. This invention can filter out noise interference from complex clinical expressions and extract neurological syndrome features by comparing them with a preset internal capsule lesion pattern. By jointly analyzing feature tensors pointing to limb paralysis with syntactic potential depth values ​​below the normal threshold, dual cross-validation of somatic motor disorders and central cognitive impairment is achieved. Furthermore, by comparing with feature thresholds of peripheral nerve or spinal cord lesions, the specificity of identifying central acute and severe illnesses such as ischemic stroke is improved, optimizing the accuracy of emergency triage.

The present invention pertains to the anti-inflammatory therapeutic effect of xantohumol in spinal cord injury (SCI). The continuous administration of xanthohumol for 1-8 weeks to SCI rats resulted in improved results in 4 clinical tests used and in lowering and faster elimination of macrophages from the SCI lesion. Since the infiltration of the SCI lesion by numerous phagocytic macrophages indicates a severe destructive inflammation of extraordinary longevity, administration of xanthohumol is neuroprotective and resulted in a better and faster recovery of the locomotor function and the strength and sensory function in the hind limbs, in a shorter period of paralysis of the urinary bladder and in the recovery of the body weight lost due to the SCI surgery. Since the traumatic brain injury (TBI) involving the white matter, and stroke involving the white matter initiate the severe destructive inflammation as in the SCI, the administration of xanthohumol is expected to be anti-inflammatory and neuroprotective in both brain diseases. Since neurodegenerative diseases including Alzheimer's disease, frontotemporal dementia and Parkinson's disease, immune mediated neuroinflammation including multiple sclerosis and neuromyelitis optica and cerebrospinal infections have inflammatory pathogenesis involving microgliosis and infiltration by macrophages, the administration of xanthohumol is expected to result in therapeutic inhibition of progression of all above diseases as well as of related neurodegenerative, immune mediated and infectious diseases of the brain and of the spinal cord.

The application discloses application of an ACSS1 gene in preparation of a drug for promoting growth of neuron axons in vitro, and belongs to the technical field of biological medicines. The application knows that expression of the ACSS1 protein presents a progressive downward trend with time passing after injury through construction of a spinal cord injury model and collection of spinal cord tissues, and then the adeno-associated virus is used to overexpress the ACSS1 in neuron primary cells, and the result shows that the length of neuron axons is significantly increased after overexpression of the ACSS1. Therefore, a substance for promoting expression of the ACSS1 gene can be used as a reagent for preparing a drug for promoting growth of neuron axons in vitro.

A miniature implantable spinal cord stimulator, comprising an electrode, a stimulator body and a control system, wherein the electrode, the stimulator body and the control system operate in cooperation, the electrode and the stimulator body are integrally connected to each other without any extension lead, the stimulator body is implanted beneath a spinous process of a spine, and the upper end of the stimulator body is flush with other spinous processes of the spine; and the control system comprises: a main controller unit, a radio frequency unit, a channel decoding unit, a transmitter unit, a power divider / combiner unit, a main power supply unit, an energy storage unit, an electrical stimulation unit, an array electrode unit and a posture recognition unit. By implanting the miniature implantable spinal cord stimulator in combination with spinal cord decompression surgery and spinal fixation surgery in an early stage after a spinal cord injury, the stimulator, having a small size and short electrode leads, avoids post-operative protrusion of an implant, and a golden window period after the spinal cord injury can thus be effectively utilized, so as to create favorable conditions for the rehabilitation of the spinal cord injury and improve rehabilitation outcomes.

The present invention relates to a novel compound YJ102 for the prevention or treatment of bladder diseases, a composition for the prevention or treatment of neuropathic pain, neuroinflammation, or inflammatory skin diseases, or for the treatment of intractable neurological diseases including neurogenic bladder. The compound YJ102 of the present invention suppresses neuropathic pain caused by various factors with superior efficacy compared to conventional drugs, improves urinary dysfunction caused by spinal cord injury, restores bladder enlargement and bladder function, suppresses the development of erythema and scaling, which are major symptoms of psoriasis in animal models of psoriasis, suppresses thickening of the epidermal layer, and suppresses splenomegaly caused by psoriasis. Therefore, it can be utilized for the treatment of various intractable neurological diseases or bladder diseases.

This invention provides the application of fasudil in the preparation of a drug for the prevention and / or treatment of neuropathic pain following spinal cord injury, relating to the field of pharmaceutical technology. Experimental data show that fasudil can significantly improve the pain behavior of rats with spinal cord injury, and its mechanism of action may be related to the inhibition of Rho / ROCK signaling pathway activation. Further studies have found that fasudil intervention during the acute phase of spinal cord injury can produce a relatively long-lasting analgesic effect, showing promising application prospects.

This invention relates to mutants of the adeno-associated virus (AAV) capsid protein. Recombinant viral vectors carrying mutants of the AAV1 capsid protein can be used for the specific expression of the introduced gene in astrocytes at the site of spinal cord injury lesions.

1. The name of the design product: front drive type lower extremity exoskeleton robot. 2. The use of the design product: auxiliary robot for lower extremity dysfunction groups such as spinal cord injury, stroke, postoperative rehabilitation patients in orthopedics, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The application discloses an experimental animal spinal column specimen dissection instrument and a dissection method. The dissection instrument comprises a fixing device, a scanning device, a cutting device and a control device. The fixing device is used for fixing the experimental animal spinal column specimen. The scanning device is used for scanning the fixed experimental animal spinal column specimen and transmitting a scanning image to the control device. The cutting device cuts the experimental animal spinal column specimen according to a cutting instruction of the control device or manually. The dissection instrument of the application integrates the fixing and cutting functions, significantly improves the efficiency and precision of the experimental animal spinal column specimen dissection, reduces the error and labor intensity of manual operation, makes the dissection process more consistent and reliable, and has high flexibility and adaptability, so that the dissection instrument can meet the dissection requirements of spinal column specimens with different sizes and curved shapes, and provide more reliable dissection samples and experimental data support for the fields of spinal column disease research, spinal cord injury repair and artificial vertebral body / intervertebral disc implant research and development.

The application discloses a nano material for targeting and driving resuscitation of dying cells, and a preparation method and application thereof. The nano material is a nano platelet vesicle with phosphatidylserine exposed to the outer layer of the membrane after nanoization and centrifugal treatment. The material is obtained by separating and enriching platelets from peripheral blood, then nanoizing the platelets, and then inducing the phosphatidylserine on the platelet membrane to evert through high-speed centrifugation. The material is used for preparing a medicine for promoting tissue repair, and the medicine promotes resuscitation of dying cells by targeting the dying cells and enhancing the membrane repair capacity of the dying cells, so as to promote tissue repair, including repair of orthopedic, muscle, skin, spinal cord or visceral tissue, especially repair of diabetic foot, skin avulsion injury, ischemic skin flap or spinal cord injury. The resuscitation of dying cells is promoted successfully without intervention of lysis programmed cell death initiation, so that the problems of off-target effect and treatment paradox existing in current mainstream small molecule inhibitor strategy are effectively solved.

The application belongs to the technical field of biomedical experimental animal model construction, and provides a rat severe spinal cord injury immediate decompression model construction method. The method establishes a standardized surgical procedure, including minimally invasive exposure through the paraspinal muscle gap, precise dura mater incision, warm saline low-pressure lavage debridement, and strict dura mater suture repair. The method realizes immediate and effective reduction of intrathecal pressure, removal of harmful substances in the injury area, and stabilization of the local microenvironment after severe spinal cord injury. The application solves the problems of instability, poor repeatability, and difficulty in simulating the clinical decompression effect caused by the non-standard dura mater incision, uncontrollable lavage, and high risk of secondary injury in the prior art. The model constructed by the method is highly standardized and has strong repeatability, and is suitable for in-depth study of the neuroprotective mechanism of dura mater incision decompression treatment for spinal cord injury.

The application belongs to the technical field of hydrogel, and particularly relates to a magnetic drug-loaded hydrogel microsphere, a preparation method and application thereof. The magnetic drug-loaded hydrogel microsphere comprises the following raw materials in mass parts: 1-40 parts of hydrogel crosslinking precursor, 2-30 parts of initiator, 0.1-1 part of magnetic nanoparticle loaded with small molecule drug, and 0-1 part of macromolecular drug. The application has the following beneficial effects: the magnetic drug-loaded hydrogel microsphere has injectability and magnetic responsiveness, can simultaneously load bioactive macromolecules and small molecules, and can realize double release of anti-inflammatory small molecules and nerve growth promoting macromolecules in the magnetic drug-loaded hydrogel microsphere, so as to synergistically reduce the harmfulness of spinal cord injury; the magnetic nanoparticle can realize flexible loading of small molecule anti-inflammatory drugs through surface functionalization; and the magnetic nanoparticle can also be used as a magnetic response clue to realize magnetic intervention on the spinal cord injury tissue in cooperation with an external static magnetic field.

The utility model discloses an adjustable spinal cord injury patient standing training auxiliary device relates to medical instrument technical field, including mechanism table, the inboard of mechanism table two sides close to left upper side's position rotationally connected with first rotation board, the upper side sliding connection of first rotation board has second rotation board, the downside swing mounting of first rotation board and second rotation board has elevating system, the two sides of mechanism table close to left side's position fixedly connected with support mechanism. The utility model discloses an adjustable spinal cord injury patient standing training auxiliary device, through elevating system drive first rotation board and the one side lifting of second rotation board make its rotation, and then make the patient be pushed to standing state, and the patient stands by first support subassembly and second support subassembly, owing to patient's body not being in the limited state, make the patient can rely on self -power to stabilize self, reach the purpose of exercise.

The application belongs to the technical field of biological medicine, and discloses a spinal cord injury treatment drug based on a LINC00311 / miR-4318 / MYLK regulation axis and an application thereof, and aims to solve the technical problems of poor targeting and large side effects of the existing anti-inflammatory treatment of spinal cord injury. The effective component of the drug is an inhibitor of LINC00311, the inhibitor is selected from one or more of shRNA, siRNA, antisense oligonucleotide and CRISPRi system, can target the specific nucleotide sequence of LINC00311, and can be used in combination with miR-4318 mimic, and is administered by intrathecal injection, slow virus carrier delivery and the like. The in-vitro experiment proves that the inhibitor can significantly inhibit the release of TNF-alpha, IL-1beta, IL-6 and other pro-inflammatory factors by microglia cells; the in-vivo experiment shows that it can significantly improve the BBB motor function score of spinal cord injury rats, and reduce spinal cord tissue edema and necrosis. The application first discloses the mechanism of the LINC00311 / miR-4318 / MYLK ceRNA regulation axis in spinal cord injury, has the advantages of novel target, significant effect and high transformation potential, and provides a new scheme for the clinical treatment of spinal cord injury.

This application discloses the application of Bcl11a in the preparation of drugs for nerve injury repair, belonging to the field of biomedical technology. The nerve injury is a central nervous system injury. Overexpression of Bcl11a promotes the transdifferentiation of an intermediate state astrocyte in the cerebral cortex into pyramidal neurons. Using Bcl11a as a molecular intervention target, targeting the M1 motor area of ​​the cerebral cortex, overexpression of Bcl11a in astrocyte subset 2 promotes the reprogramming of astrocytes into pyramidal neurons, promoting the repair of central nervous system injury. Using spatial transcriptome sequencing technology and single-cell sequencing technology, the dynamic trajectory between astrocytes and pyramidal cells was depicted, supporting the transformation of an intermediate transitional state astrocyte in the cortex into pyramidal cell subset 3 after spinal cord injury, indicating that Bcl11a and Zmart4 are key transcription factors in the connection between pyramidal cells and glial cells.

This invention discloses a method for preparing a porous electrospun scaffold based on polycaprolactone and acetylglucan, and its application. The preparation method includes: dissolving glucan in an organic solvent, reacting it with pyridinium 4-methylbenzenesulfonate and 2-methoxypropylene under nitrogen protection, followed by termination, precipitation, centrifugation, washing, and freeze-drying to obtain acetylated glucan; subsequently, dissolving it and a polymer separately in a mixed solvent of dichloromethane and N,N-dimethylformamide, and spinning it into a film using a bilayer coaxial electrospinning technique to prepare a porous scaffold. This scaffold exhibits strong stability, excellent biocompatibility, and the ability to promote tissue repair, making it suitable for the treatment of spinal cord injuries. In particular, it can precisely target the injured site via in-situ drug delivery, reducing the impact on normal tissues and achieving highly efficient repair effects.

The application discloses an implantable brain-computer interface control spinal cord electrical stimulation patient improvement effect analysis method, relates to the technical field of medical rehabilitation, and the method comprises the following steps: multi-dimensional cooperative data acquisition: electrodes are implanted below target brain areas and spinal cord injury segments, detection elements are installed at key positions of exoskeletons, sensors are attached to preset muscle groups of lower limbs, electroencephalogram signals, SCS stimulation parameters, EXS motion data and neuromuscular response data are synchronously collected, and time correlation marks are embedded; by adopting the mode that multi-source signal preprocessing is combined with a multi-modal intention recognition model, the reliability of motion intention decoding is remarkably improved, in the signal preprocessing stage, an adaptive filtering algorithm combining Kalman filtering and wavelet threshold denoising is used, and SCS electrical stimulation interference, EXS motor noise and physiological noise in the electroencephalogram signals are effectively removed.

The present application relates to nerve growth factor inducible protein or its gene in the preparation of treating spinal cord injury drug application. The present application proves that the lentivirus of overexpressing nerve growth factor inducible protein gene can promote the regeneration of oligodendrocytes, and then can effectively improve the recovery of motor function of spinal cord injury animal model through cell experiment and animal experiment.

This invention discloses a spinal cord injury testing auxiliary device, belonging to the field of injury testing technology. The spinal cord injury testing auxiliary device includes a basic shell, a body position adjustment system, a head-mounted detection system, and a safety protection system. A seat body is located on top of the basic shell, possessing height self-adjustment capabilities, enabling personalized testing plans for patients of different body types. The body position adjustment system, through the coordinated work of a bidirectional threaded rod and a moving block, achieves linked adjustment of the seat tilt angle and foot pedal position, ensuring that patients of various body types can obtain the optimal testing position. The facial fitting mechanism employs temperature-controlled expansion technology, allowing the flexible airbag to adapt to different facial contours. The elastic buffer structure of the head fixation mechanism ensures wearing comfort and stability. This comprehensive self-adjustment significantly improves the applicability of the device and the patient experience, ensuring that different individuals can complete the testing under optimal conditions, effectively eliminating data deviations caused by device compatibility issues.

The method for screening key genes, metabolites and pathways of spinal cord injury based on multi-omics joint analysis comprises the following steps: (1) establishing an experimental animal model of spinal cord injury, setting a spinal cord injury group and a sham operation group, and observing the motor function of the injury side of the experimental animal by motor function score; (2) extracting spinal cord tissue samples of each group, performing histological detection by HE staining and Nissl staining, performing transcriptome sequencing by RNA-seq technology, and performing metabolome sequencing by LC-MS / MS technology; (3) applying bioinformatics to analyze the biological significance of a single omics; (4) integrating transcriptome data and metabolome data for joint analysis, exploring key genes, metabolites and pathways affecting the pathophysiological mechanism of spinal cord injury, and constructing a gene-metabolite network interaction diagram. The molecular mechanism affecting the pathophysiological process of spinal cord injury and the relationship between injury microenvironment genes and metabolites are disclosed, which provides a research basis for multi-omics joint analysis and action of spinal cord injury.

This invention relates to the fields of biomedicine and tissue engineering, and discloses a DNA hydrogel-dental pulp stem cell complex, its preparation method, and its application in the preparation of drugs for spinal cord injury repair. The DNA hydrogel comprises multiple DNA chains, at least one of which contains multiple Apt19s sequences. These Apt19s sequences specifically bind to the ALPL protein on the surface of dental pulp stem cells. This invention co-incubates the DNA hydrogel with dental pulp stem cells, utilizing the high affinity of aptamers and DNA self-assembly to construct a DNA hydrogel-dental pulp stem cell complex that integrates specific adhesion, anti-inflammatory, and antioxidant effects of dental pulp stem cells, thereby achieving effective repair of spinal cord injuries.

The embodiment of the application discloses a kind of identification methods of spinal cord injury based on deep learning, it is characterized in that: including the following steps: obtaining the MRI image of the spinal cord of several personnel, the MRI image includes normal spinal cord and injury spinal cord, and the MRI image is preprocessed, training data set is constructed;The training data set is input to convolutional neural network and is learned, and the hyperparameter of the convolutional neural network is updated using improved cuckoo optimization algorithm, and the convolutional neural network that learns is completed is obtained;The MRI image of the spinal cord of the personnel to be detected is obtained, it is input to the convolutional neural network that learns is completed, and the spinal cord injury detection result and corresponding injury position of the personnel to be detected are obtained.The present application realizes the detection of spinal cord injury based on deep learning technology, effectively solves the technical problems that the accuracy and efficiency of the existing technology are not high in spinal cord injury detection, realizes the automatic detection of spinal cord injury, and does not depend on professional medical personnel.

This invention discloses a material-empowered stem cell-derived exosome, its preparation method, and its application, belonging to the field of biomedical technology. The method involves the following steps: mesenchymal stem cells are seeded onto a substrate fused with N / VE-cadherin-Fc fusion protein and cultured to assemble into aggregates; the aggregates are dynamically cultured under staged shear stress; the culture medium is collected, and the exosomes are separated and purified. The preparation method of this invention overcomes the shortcomings of existing mesenchymal stem cell exosome preparation technologies, such as low yield and insufficient neurovascular repair function. It significantly activates exosome biogenesis-related molecular pathways, increases exosome yield by approximately four times, and is rich in miRNAs related to neurovascular repair. It can significantly improve neurological deficits in stroke mice and hindlimb motor function in rats with spinal cord injury, providing a highly efficient and stable cell-free therapeutic strategy for the treatment of central nervous system injuries.

This utility model discloses an electrostimulation therapy helmet with rehabilitation functions, belonging to the field of rehabilitation assistive device technology. It includes a front shell and a rear shell, which are connected together by a head circumference adjuster to form a helmet body adapted to the human head. A strap extending towards the top of the head is provided on the helmet body, and the strap is equipped with a tension adjuster and a head cover. Electrode plates are provided on the inner surfaces of both the rear shell and the head cover, and the inner surfaces of the electrode plates are designed to contact the human head. Specifically, the electrode plates on the inner surface of the rear shell correspond to the balance zone of the human head, and the electrode plates on the inner surface of the head cover correspond to the motor zone of the human head. This utility model provides electrostimulation therapy to the balance and motor zones for people recovering from spinal cord injuries, myelitis, or strokes, helping patients obtain effective rehabilitation stimulation in a home environment and accelerating the rehabilitation process.