318 results about "Spinal cord lesion" patented technology

Fluorine-containing amino acid derivatives represented general formula (I), pharmaceutically acceptable salts thereof or hydrates of the same, wherein X1 represents hydrogen or fluorine; and R1 and R2 are the same or different and each represents hydrogen or lower C1-10 alkyl. These compounds are useful as drugs, in particular, group 2 metabotropic glutamate receptor agonists for treating and preventing psychiatric disorders such as schizophrenia, anxiety and associated diseases, depression, bipolar disturbance and epilepsy, and neurological diseases such as drug addiction, cognition disorder, Alzheimer's disease, Huntington's chorea, Parkinson's disease, motility disturbance associating muscular stiffness, cerebral ischemia, cerebral insufficiency, spinal cord lesion and head disturbance.

The invention provides a method for treating CNS disorders by administering a neural stem cell composition and a mesenchymal stem cell composition on opposing sides of the blood brain barrier. The neural stem cell composition is administered to the central nervous system, while the mesenchymal stem cell composition is administered to the circulatory system, such as by intravenous injection. The method finds use in the treatment of degenerative GNS disorders, as well as traumatic CNS disorders such as stroke and spinal cord injury.

One aspect of the invention provides a three-dimensional scaffold including at least one layer of highly-aligned fibers. The at least one layer of highly-aligned fibers is curved in a direction substantially perpendicular to a general direction of the fibers. Another aspect of the invention provides a method for fabricating a three-dimensional scaffold. The method includes: electro spinning a plurality of fibers to produce at least one layer of highly-aligned fibers and forming the at least one layer of highly-aligned fibers into a three-dimensional scaffold without disturbing the alignment of the highly-aligned polymer fibers. A further aspect of the invention provides methods for using a three-dimensional scaffold to treat nerve or spinal cord injury.

This application discloses a light-responsive hydrogel-based platform that can modulate multiple microenvironmental signals to direct the differentiation of human induced pluripotent stem cell-derived neural progenitor cells (hiPSC-NPCs) into neuronal cells. The invention provides novel methods for directing differentiation of neural stem cells into neurons useful for treatment of degenerative diseases or disorders, including but not limited to Alzheimer's, Parkinson's, or spinal cord injury (SCI).

At least one electrical brain signal is received from a patient and is demultiplexed into an efferent motor intention signal and at least one afferent sensory signal (such as an afferent touch sense signal and/or an afferent proprioception signal). A functional electrical stimulation (FES) device is controlled to apply FES to control a paralyzed portion of the patient that is paralyzed due to a spinal cord injury of the patient. The controlling of the FES device is based on at least the efferent motor intention signal. A demultiplexed afferent touch sense signal may be used to control a haptic device. The afferent sensory signal(s) may be used to adjust the FES control.

The present invention provides methods, compounds and kits for increasing the viability of cells. The methods involve treating cells that make up a tissue graft with erythropoietin before, during or after delivery or administration. The method can employ cells of different types, including cells of neural or paraneural origin, such as adrenal chromaffin cells. Also useful are cell lines grown in vitro. Cells not of neural or paraneural origin, such as fibroblasts, may also be used following genetic alteration to express a desired neural product such as a neurotransmitter or a neuronal growth factor. The method is used to treat neurological diseases such as Parkinson's disease, Alzheimer's disease, Huntington's disease, epilepsy, and traumatic brain or spinal cord injury.

The invention discloses a method and a device for injury potential compensation after spinal cord injury. The method comprises the following steps of: detecting an injury potential after spinal cord injury, observing the value of injury potential in real time, and controlling a stimulation voltage according to the observed value of injury potentials to allow the injury potential close to zero. The method achieves injury potential compensation to ensure that the spinal cord microenvironment is relatively stable. The device for implementing the compensation method comprises four stimulating electrodes, a stimulation voltage generating circuit (2), an injury potential measuring electrode, an injury potential amplifying circuit (4) and voltage display equipment (5). The stimulation voltage generating circuit (2) generates an adjustable stimulation voltage, the stimulation voltage is applied onto the spinal cord of a patient through the stimulating electrodes to stimulate the spinal cord, the injury potential measuring electrode is used for measuring an injury potential, the injury potential amplifying circuit (4) magnifies the injury potential and filters off high-frequency interference in the injury potential, the voltage display equipment (5) displays the value of magnified injury potential, and the stimulation voltage is regulated according to the value of magnified injury potential.

The invention discloses a method for establishing a severe spinal cord injury prognosis prediction model, and the method is characterized by comprising the following steps: extracting clinical data of cases of patients diagnosed as spinal cord injury: 1) incorporating the following clinical characteristics; 2) preprocessing the clinical features: processing missing data through different filling methods according to the types of the clinical features; 3) incorporating an algorithm combination of a feature selection method and a machine learning classification algorithm, wherein the feature selection method is used for screening clinical features with significant prediction values, and the selected clinical features are used for training the machine learning classification algorithm; 4) selecting the algorithm combination with the maximum area AUC under the micro average curve from the prediction performance of the algorithm combination in the step 3) in the training data set, and integrating the algorithm combination by using a stacking method to obtain a prediction model. The invention has accurate and objective performance for predicting the prognosis of the patient with severe spinal cord injury.

The invention relates to the technical field of tissue engineering, in particular to a tissue engineering material for nerve injury repair as well as a preparation method and application of the tissueengineering material. The tissue engineering material for nerve injury repair is a linear ordered collagen stent of crosslinked N-cadherin. The tissue engineering material prepared by crosslinkingtheN-cadherin in the linear ordered collagen stent can efficiently induce neural stem cells to migrate towards an injury region, enrich neutral stem cells in the injury region, effectively inhibit deposition of inhibitors such as chondroitin sulfate proteolgycan and promote differentiation of the neutral stem cells towards neurons so as to promote recovery of electrophysiological and motion functions; and meanwhile, the N-cadherin crosslinked linear ordered collagen stent also has stable and ordered topological structures and excellent mechanical properties and thus can be used for repairing nerve injuries such as spinal cord injury.

The invention discloses an M2 type bone marrow macrophage exosome, application thereof and a spinal cord injury treatment preparation, and belongs to the technical field of spinal cord injury treatment. The M2 type bone marrow macrophage exosome highly expressing the OTULIN protein can play roles in improving a local ischemia and hypoxia environment after spinal cord injury and promoting revascularization, and is more beneficial to nerve regeneration and survival of local tissues. An exosome sustained-release system is formed by combining with light-cured hydrogel and is applied to local spinal cord injury, so that the treatment efficiency of the exosome is improved, liver and kidney metabolism is avoided, long-time action can be realized by continuous sustained release, and functional recovery of mice after spinal cord injury is remarkably improved.

The invention discloses a human spinal cord neural stem cell induced differentiation culture system and method.The human spinal cord neural stem cell induced differentiation culture system mainly comprises a spinal cord neural stem cell induction culture medium and a spinal cord neural stem cell maintenance culture medium, and the spinal cord neural stem cell induction culture medium takes an N2B27 culture medium as a basic culture medium; every 10 ml of the N2B27 culture medium comprises 10 to 12 [mu] l of a BMP inhibitor, 10 to 12 [mu] l of an ALK5 inhibitor, 10 to 12 [mu] l of a GSK-3 inhibitor, 10 to 12 [mu] l of FGF2 and 10 to 12 [mu] l of FGF8. The spinal cord neural stem cell maintenance culture medium takes an N2B27 culture medium as a basic culture medium, and every 50 ml of the N2B27 culture medium comprises 10-12 [mu] l of an ALK5 inhibitor, 15-18 [mu] l of a GSK-3 inhibitor and 1-1.2 [mu] l of a Hedgehog agonist. The induced differentiation culture system is used for carrying out induced differentiation culture on the human embryonic stem cells, the human spinal cord NSC cells can be directionally differentiated and formed, and the human spinal cord neural stem cells have multidirectional differentiation potential, so that the human spinal cord development/regeneration related cell process can be better understood; the method is of great significance to follow-up treatment strategies for diseases such as human spinal cord injury and degeneration.

The invention relates to a dendritic cell bacterin, in particular to a dendritic cell bacterin which can effectively promote the functional recovery of spinal cord injury and a manufacturing method thereof.

The invention discloses a method for promoting mesenchymal stem cells to differentiate into neurons, comprising the following steps: 1) preparing human mesenchymal stem cells and subculturing; 2) differentiating the passaged human mesenchymal stem cells into human mesenchymal stem cells in a differentiation medium-neurosphere; 3) differentiating the human mesenchymal stem cell-neurosphere into neurons in an induction culture medium. Neurotrophic factors are added to the induction culture medium to promote the differentiation into neurons, and the results show that expressing mature nerve marker (MAP2ab) and immature nerve marker (beta-tubulin III) were significantly improved relative to the blank control. The method of the invention provides a certain direction for a new treatment method for promoting the repair of spinal cord injury.