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Composite mesenchymal stem cell functional collagen scaffold and applications thereof

A collagen scaffold and stem cell technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as nerve dysfunction that cannot be effectively solved

Inactive Publication Date: 2018-08-21
BEIJING ZKZKTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current clinical treatment methods are mainly surgical treatment and drug treatment. Although the progression of the disease can be alleviated to a certain extent, they cannot effectively solve the problem of neurological dysfunction.

Method used

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  • Composite mesenchymal stem cell functional collagen scaffold and applications thereof
  • Composite mesenchymal stem cell functional collagen scaffold and applications thereof
  • Composite mesenchymal stem cell functional collagen scaffold and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0101] Embodiment 1, the preparation of the functional collagen scaffold of composite mesenchymal stem cells

[0102] 1. Isolation and culture of placental mesenchymal stem cells

[0103] 1. Take the placenta of the full-term caesarean section fetus (according to the hospital's regulations and the consent of the family members), and separate the placental chorionic tissue.

[0104] 2. Wash the placental chorionic tissue obtained in step 1 with PBS buffer and cut it into small pieces, digest it with 0.1% (mass fraction) type II collagenase at 37° C. for 90 min, and then use 10% (volume percentage) fetal bovine serum The L-DMEM culture solution was pipetted repeatedly to stop the digestion, and the cell suspension was obtained by passing through a 200-mesh sieve, and the cell suspension was centrifuged at 160 g for 5 min, the supernatant was discarded, and the cell pellet was collected.

[0105] 3. Resuspend the cell pellet obtained in step 2 with L-DMEM medium (containing 10mL...

Embodiment 2

[0127] Example 2. Application of Collagen Scaffold Composite with Mesenchymal Stem Cells to Treat Canine Acute Spinal Cord Transection Injury

[0128] 1. Preparation of canine acute spinal cord transection injury model

[0129] Experimental animals: 1.5-year-old female Beagle dogs (body weight 8-10kg).

[0130] After the experimental animals were anesthetized, they were randomly divided into 6 groups, 6 animals in each group, and the following operations were performed:

[0131] Control group I: The T7-T9 lamina was opened to expose the spinal cord tissue, the spinal cord at the T8 segment was resected by 5 mm, no collagen scaffold was transplanted, and the dura mater was tightly sutured.

[0132] Control group II: Open the T7-T9 lamina to expose the spinal cord tissue, resect the spinal cord at the T8 segment 5 mm, transplant the 5 mm long and 5 mm total diameter collagen scaffold bundle prepared in Step 3 of Example 1, and tightly suture the dura mater.

[0133] Experiment...

Embodiment 3

[0161] Example 3. Application of Collagen Scaffold Composite with Mesenchymal Stem Cells to Treat Dogs with Old Spinal Cord Transection Injury

[0162] 1. Preparation of canine old total transection injury model of spinal cord

[0163] Experimental animals: 1.5-year-old female Beagle dogs (body weight 8-10kg).

[0164] 1. After anesthetizing the experimental animals, open the T7-T9 lamina to expose the spinal cord tissue, resect the spinal cord at the T8 segment by 5 mm, and suture the dura mater tightly.

[0165] 2. At the 9th week (after 2 months) after completing step 1, the experimental animals were anesthetized and randomly divided into 6 groups with 7 animals in each group, and the following operations were performed:

[0166] Control group I: The skin was cut along the original incision, and the epidural and subdural scar tissue was excised under a microscope until the normal spinal cord tissue at both ends was exposed. No collagen scaffold was transplanted, and the du...

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Abstract

The invention discloses a composite mesenchymal stem cell functional collagen scaffold and applications thereof. According to the present invention, isolated animal fascia is soaked sequentially withacetone, a sodium deoxycholate solution, a Triton X-100 solution, a pepsin aqueous solution and a papain solution, and free-drying is performed to obtain the composite mesenchymal stem cell functionalcollagen scaffold; the composite mesenchymal stem cell functional collagen scaffold is used for acute spinal cord injury and old spinal cord injury, and the comprehensive evaluation results from various aspects show that the composite mesenchymal stem cell functional collagen scaffold can substantially promote the movement function recovery, nerve regeneration, axon myelination and synapse formation of acute and old spinal cord injury dogs; and the functional biomaterial can provide great significance in the repair of spinal cord injury, and can provide theoretical support for the applicationof functional biomaterials in the future.

Description

technical field [0001] The invention relates to a functional collagen scaffold of composite mesenchymal stem cells and application thereof. Background technique [0002] Spinal cord injury (SCI) is a serious trauma to the nervous system, which can cause sensory and motor disturbances below the injury, abnormal reflexes, and incontinence and other corresponding pathological changes. A big problem. The current clinical treatment methods are mainly surgical treatment and drug treatment. Although the progression of the disease can be alleviated to a certain extent, they cannot effectively solve the problem of neurological dysfunction. [0003] In recent years, with the deepening understanding of the pathophysiological process of spinal cord injury, cell transplantation has shown a good application prospect in the treatment of spinal cord injury. Cell transplantation after spinal cord injury has several purposes: filling the damage cavity; replacing dead cells (such as providin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24
CPCA61L27/24A61L27/3834A61L27/3878A61L2430/32A61L2430/40
Inventor 戴建武赵燕南陈冰肖志峰韩素芳李星
Owner BEIJING ZKZKTECH CO LTD
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