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Herniorrhaphy sheet and preparation method thereof

A technology of patch and skin patch, applied in the field of hernia patch and its preparation, can solve the problems of large material damage, unqualified, easy to have residual material cytotoxicity, etc.

Active Publication Date: 2016-10-12
SUZHOU HENRUI DISHENG MEDICAL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (2) Use trypsin to separate the epidermis and dermis, NaOH strong alkali and detergent SDS to decellularize. Among them, trypsin, NaOH strong alkali and detergent SDS have great damage to the material, and SDS is also difficult to clean, and it is easy to cause residues. The cytotoxicity of the material is unqualified;
[0009] (3) Repeated freezing and thawing and ultrasonic methods are used to remove cells, which has poor decellularization effect;

Method used

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  • Herniorrhaphy sheet and preparation method thereof
  • Herniorrhaphy sheet and preparation method thereof
  • Herniorrhaphy sheet and preparation method thereof

Examples

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preparation example Construction

[0103] The inventor has developed a hernia patch and a preparation method thereof for the first time through extensive and in-depth research. The method comprises the steps of: peeling and sorting, degreasing and decellularization, cutting, and irradiation sterilization. The preparation method of the hernia patch proposed by the present invention avoids the use of strong acid and alkali and enzymes, and can retain the natural three-dimensional collagen scaffold fiber structure of the pig dermis matrix, so that the hernia patch has good mechanical properties to strengthen or repair the defective tissue. The prepared hernia mesh can completely retain the natural structure of pig dermal matrix, has good tissue compatibility, and has no hidden danger of virus transmission.

[0104] the term

[0105] As used herein, the terms "biological patch cutting device" and "cutting device" all refer to the same device.

[0106] Hernia mesh

[0107] In one embodiment, a cell-free hernia pa...

Embodiment 1

[0200] Step 1. Peeling and sorting

[0201] Provide a dehaired pigskin with the subcutaneous tissue removed, with figure 1 The thickness measuring system shown measures its thickness, and determines the area of ​​uniform skin thickness according to the measurement data (with 0.5mm as the step, the thickness of the skin is divided into 0.8-1.3mm, 1.3-1.8mm, 1.8-2.3mm , 2.3 ~ 2.8mm, 2.8mm or more can be used), divide the skin slices according to the area, and then use a large dermatome to cut off the epidermis and basement membrane to obtain dermal slices with uniform regional thickness.

[0202] Step 2, first degreasing and then decellularization

[0203] Select the process parameters of the dermis skin sheet prepared in step 1 according to the following table, and complete the processing of each step in the order of the following table, and the processing temperature is 20°C:

[0204]

[0205]

[0206] Step 3. Cutting

[0207] The degreased and decellularized porcine ...

Embodiment 2

[0217] Step 1. Peeling and sorting

[0218] Provide a dehaired pigskin with the subcutaneous tissue removed, with figure 1 The thickness measurement system shown measures its thickness, and determines the area of ​​uniform thickness of the skin according to the measurement data (with a step of 1mm, the thickness of the skin is divided into 0.8-1.8mm, 1.8-2.8mm, 2.8mm or more, etc. ), divide the skin slices by region, and then use an electric dermatome to cut off the epidermis and basement membrane to obtain dermis skin slices with uniform regional thickness.

[0219] Step 2: decellularize first and then degrease

[0220] Select the process parameters of the dermis sheet obtained in step 1 according to the following table, and complete the processing of each step in the order of the following table, and the processing temperature is 30°C:

[0221]

[0222] Step 3. Cutting

[0223] The decellularized degreased porcine dermal matrix prepared in step 3, using figure 2 The ...

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Abstract

The invention discloses a herniorrhaphy sheet and a preparation method thereof. The preparation method comprises the following steps of peeling sorting, degreasing and decellularization, cutting and irradiation sterilization. The preparation method of the herniorrhaphy sheet provided by the invention has the advantages that the use of strong acid, strong alkali and enzymes is avoided; a natural three-dimensional collagen scaffold fiber structure of the pig skin substrate can be remained, so that the herniorrhaphy sheet has good mechanical performance so as to achieve the effects of reinforcing or restoring defect tissues; the prepared herniorrhaphy sheet can completely remain the natural structure of the pig skin substrate; good tissue compatibility is achieved; no virus spreading hidden danger exists.

Description

technical field [0001] The invention relates to the technical field of tissue engineering medical biomaterials, in particular to a hernia patch and a preparation method thereof. Background technique [0002] Hernia, commonly known as a hernia, means that a part of a human tissue or organ leaves its original site and enters another site through a gap, defect or weakness in the human body. Hernias include umbilical hernias, direct inguinal hernias, indirect hernias, and incisional hernias. The treatment of hernia mainly includes conventional treatment, conservative treatment and surgical treatment, wherein the application of conventional treatment and conservative treatment is relatively small, generally only applied to infants under one year old and elderly people with poor tolerance. Adult hernias do not heal on their own, and surgery remains the only cure. The method of surgical treatment of hernia is hernia repair, which mainly includes traditional repair and tension-fre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36
Inventor 刘博文周宁辉王志东朱鑫建俞鸿飞
Owner SUZHOU HENRUI DISHENG MEDICAL CO LTD
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