113 results about "Dermal matrix" patented technology

A breast implant includes an elastomer shell; a dermal matrix disposed on the implant shell; and a support interposed between the elastomer shell and dermal matrix to separate the elastomer shell and dermal matrix. A process for making a breast implant includes disposing an elastomer shell in a support; and disposing a dermal matrix on an outer surface of the support. A method of using the breast implant include disposing the breast implant in a subject and attaching the support to tissue of the subject.

An object of the present invention is to provide a method for producing an ADM suitable as a scaffold for a cultured skin, that is, a separation and decellularization method that enables various types of extracellular matrixes such as a basement membrane to be preserved, allows the epidermal layer to be peeled off easily, and does not cause any damage to the dermal matrix. The present invention relates to a skin separation and decellularization method comprising a step of freeze thawing harvested skin and then separating the skin into epidermis and dermis by a treatment with hypertonic saline, and a step of washing the separated dermis.

The present invention relates to a composition consisting of a combination of peptides that regenerate the dermal matrix and a rapid acting muscle relaxant derived from the extract of the plant, Acemella oleracea. By limiting cutaneous deformation caused by the contraction of facial muscles the extract increases the efficiency of the peptides in reducing expression lines.

Flowable matrix compositions and methods of their use and manufacture are provided. Exemplary compositions may include a flowable, syringeable, putty-like form of acellular human dermal matrix. In some cases, compositions may include a moldable acellular collagen extracellular matrix. In use, the matrix compositions can be used to fill or treat skin voids, channel wounds, and other soft tissue deficiencies.

The invention provides a method for constructing tissue engineering skin. In the method, human epidermal stem cells and hypodermal fibroblasts are taken as seed cells, and a homologous acellular dermal matrix modified by a human placental type IV collagen is taken as a stent, so that active dual-layer engineering skin is compounded and constructed by using a gas-liquid interface separate culture method. The tissue engineering skin constructed by using the method is closer to the structure of natural skin; and as proved by histomorphology, the tissue engineering skin has an epidermal and hypodermal double-layer structure, wherein the hypodermal collagen stent has a complete structure, an epidermal layer is provided with a plurality of layers of cells of different differentiation degrees, and the morphological requirement of the tissue engineering skin is met.

Flowable matrix compositions and methods of their use and manufacture are provided. Exemplary compositions may include a flowable, syringeable, putty-like form of acellular human dermal matrix. In some cases, compositions may include a moldable acellular collagen extracellular matrix. In use, the matrix compositions can be used to fill or treat skin voids, channel wounds, and other soft tissue deficiencies.

The invention is based on selective inhibition of the enzyme (MMP-1), which causes the dermal matrix damage in humans, while sparing the enzyme(s) (MMP-9 and perhaps MMP-2) which not only do not cause the damage (based on extrapolation from our in vitro collagen gel system to real skin) but actually "clear away" the damage produced by MMP-1 to restore normal function to the skin. Matrix metalloproteinase-1 (MMP-1; fibroblast collagenase) is induced by UV radiation from the sun and is naturally elevated in old age. Human fibroblasts exposed to the degradation products of MMP-1 contract collagen, but when this debris is removed from their environment, the fibroblasts behave normally. Inhibiting MMP-1 but sparing enzymes that remove the debris improves human skin after onslaught from solar UV radiation, old age, and acne.

A stent may help to reconstruct tissue in a vessel by causing the tissue to re-epithelialize. The stent may include a biodegradable frame and a sheet that coats the frame. The sheet may contain a biological material and may flex in unison with the frame in a radial direction. When placed in a vessel, the stent may at least partially conform to at least a portion of a vessel wall. The stent may be capable of being absorbed over a period of time, such as five years, one year, or six months. The stent may flex as the vessel wall dilates and constricts. The stent may be placed in any type of vessel including an artery and mobile vessels. The biodegradable frame may be made of a poly-lactide and / or magnesium. The sheet may contain a biological material including biologic arterial graft and / or an acellular dermal matrix.

The present invention relates to the Synthesis of Triterpenoid peptides and mechanism of action for Anti ageing and skin care. The present invention is directed towards anti-aging skin care compositions comprising peptides which are made by linking herbal actives to a pentapeptide for enhanced anti ageing activity by regenerating the dermal matrix. In detail, the present invention relates to the synthesis of Triterpenoid peptides, providing an enhanced and synergistic activity for reducing the consequences of ageing such as appearance of fine expression lines and wrinkles on the skin by cosmetic modes of application. The Triterpenoid peptides of the present invention with its novel dual action mode can be used for skin ageing & collagen insufficiency. Its Triterpenoid group acts by preventing oxidation and excess activity of serine proteases like elastase and collagenase that result in wrinkling of skin. With added peptides which boost the collagen and other matrix protein, Triterpenoid peptides provide a complete protection against pre mature ageing and functions as a best anti ageing ingredient.

A composition consisting of a combination of peptides that regenerate the dermal matrix and a rapid acting muscle relaxant derived from the extract of the plant, Acmella oleracea. By limiting cutaneous deformation caused by the contraction of facial muscles the extract increases the efficiency of the peptides in reducing expression lines.

The invention provides a dura graft and a preparation method thereof. The dura graft is prepared from an acellular dermal matrix. The acellular dermal matrix is obtained from allogenic skin by crosslinking treatment, digestion treatment with a protease solution, decellularization with a hypertonic saline solution containing a surfactant, DNA degradation, amino acid nutrition treatment and freeze drying. The invention also provides applications of the dura graft in preparing repair materials used in the treatment of dural damage. The acellular dermal matrix is obtained from human skin tissue and undergoes physicochemical and biochemical treatment, so that all the components that may cause immunological rejection are removed. A fibrous stereoscopic scaffold structure of the original tissue is completely reserved, and new vessels and fibroblasts rapidly grow in the dura graft after implantation. Therefore, the dura graft has good clinical effects.

The invention discloses tissue engineering skin which comprises seed cells and an acellular allogenic dermal matrix, wherein the seed cells and the acellular allogenic dermal matrix form a skin appendage; the seed cells are arranged, spread flat and printed at corresponding positions on the front side of the acellular allogenic dermal matrix according to an anatomical structure of normal skin by using a 3D (Three-dimensional) printer. The invention also discloses a method for preparing the tissue engineering skin. The invention provides perfect tissue engineering skin, and the tissue engineering skin can be rapidly constructed in a short time in an operating room (the operation is finished). Meanwhile, the tissue engineering skin comprises the seed cells and dermal matrix, the seed cells are basal layer cells, have the activities of multipotential stem cells and multipotential differentiation functions and are arranged and printed in the corresponding positions according to the anatomical structure of the normal skin, the skin appendage is easily formed, the cure rate is accelerated after the operation, and functions of the skin are restored.

Soft tissue repair grafts are provided for supporting, covering, and / or retaining an implant positioned in the body of a subject. The grafts are particularly suitable for use for pre-pectoral breast reconstruction with a breast implant or tissue expander. The grafts include positional notches for more accurate positioning in a subject. The grafts also include at least one cuff element which is folded to form a reinforced folded edge for suturing the graft more securely to adjacent tissues than previously known grafts. The grafts also include a plurality of arcuate slots which form a plurality of circular patterns arranged concentrically about a focal point, thereby enabling the grafts to expand without tearing and to conform more closely to the implant and / or adjacent body tissues such as the breast pocket, than previously known grafts. Acellular dermal matrices are particularly suitable for making the soft tissue repair grafts.

The invention relates to a hernia patch, a preparation method and application thereof in herniorrhaphy. The hernia patch is prepared by completely removing all cells possibly causing host immunological rejection from an allogenic skin raw material and integrallty retaining extracellular matrix with the same original tissue structure. The acellular allogenic dermal matrix has tensile strength of 3.0MPa-7.0MPa, stitching strength of 18N-22N, tensile elongation of 10%-30% and bursting strength of 20KPa-30KPa. The hernia patch provided by the invention has the effect of inducing tissue generation,and can be recognized as autologous tissue by human tissue cells after implantation, and soon new blood vessels and fibroblasts can grow into the patch to guide orderly growth of cells along the collagen frame, thus reaching the purpose of supplementation, especially fast repair. The patch is non-degradable within 24 weeks after filling.

The invention belongs to the field of xenogenic acellular dermal matrixes and preparation methods thereof. The preparation method of a freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix is characterized by comprising the following steps: chipping a skin graft of piglet skin, and drawing the skin graft; perforating with laser; cleaning by adopting a buffer solution; performing freeze-thawing, and adding the buffer solution which is enough to immerse the skin graft for ultrasonic oscillation so as to remove destroyed dead cells and cell debris to maintain the completeness of structure and base membrane in acellular derma; cleaning with purified water, adding sulfadiazine zinc less than or equal to 0.12mg / cm<2>, and freezing at minus 20 DEG C; and sealing, disinfecting and sterilizing. The freeze-thawing xenogenic laser microporous irradiated acellular dermal matrix prepared with the method is characterized in that: the thickness is between 0.3mm and 0.4mm, the pitch is between 1.0mm and 2.0mm, and the pore diameter is between 500um and 808um.

The invention relates to a preparing method of a heterogeneous acellular dermal matrix substrate with good biocompatibility. The method concretely comprises the following steps of (1) split thickness skin graft preparation; (2) epidermis and dermis separation; (3) acellular processing; (4) cross linking; (5) virus inactivation; (6) refining; (7) Co-60 irradiation sterilization. The ecellular tissue engineering materials obtained by the method provided by the invention has the advantages that the toxicity is stable, and the cell compatibility is better.

The invention relates to acellular polysaccharide dermal matrix materials and a production method and application thereof. The acellular polysaccharide dermal matrix materials comprise acellular dermal matrix materials and polysaccharide, wherein the polysaccharide solution is composite solution of carboxymethyl chitosan, chondroitin sulfate and hyaluronic acid. The production method includes: selecting encapsulated and sterilized PADM, weighing, compositing carboxymethyl chitosan, compositing chondroitin sulfate, crosslinking with glutaraldehyde, compositing hyaluronic acid, washing with a PBS buffer solution, encapsulating, sterilizing, and the like. Functional materials can be added into the acellular polysaccharide dermal matrix materials to produce biologic dressings, composite transplant stents and tissue engineering epidermis stents. The acellular polysaccharide dermal matrix materials are good in in-vitro degradability, high in tensile strength, high in water vapor permeability, high in porosity and excellent in antibacterial performance.

The present invention relates to a production method for cryopreserved acellular dermal matrix and to cryopreserved acellular dermal matrix produced thereby, and more specifically it relates to a method in which a cryopreservation agent is made by adding sucrose to basic components consisting of glycerol and a basic solution and in which the resulting solution is used in the cryopreservation of skin tissue from which the cells in the epidermis and dermis have been removed, and relates to cryopreserved acellular dermal matrix produced thereby.

A method for manufacturing an animal acellular tissue matrix material and a tissue matrix material manufactured by the same. The tissue matrix material manufactured by the method retains an original basic scaffold structure of a tissue extracellular matrix, with an antigen causing immunological rejection in a human body being effectively removed from the animal tissue. An animal dermal matrix manufactured by the method retains the biological integrity of a natural dermal tissue matrix and can be used for restoration and repair of lesion and missing tissues.

The invention discloses a nano silver-porcine acellular dermal matrix (PADM) biological dressing and a preparation method thereof. The biological dressing comprises PADM and nano-silver particles assembled on the PADM, wherein, the content of the nano-silver particles is 435.66-447.66 mu g / g, and the particle size of the nano-silver particles is 55-75nm. The preparation method of the biological dressing comprises the following steps: (1) reducing silver nitrate with trisodium citrate to prepare nano silver solution; (2) carrying out gradient dehydration with alcohol and ultrasonic treatment on the PADM; and (3) placing the treated PADM obtained from the step (2) in the nano silver solution for self-assembly, thus finally obtaining the nano silver-PADM biological dressing. The biological dressing obtained in the invention has the advantages of strong antibacterial property, high biological safety and good tissue compatibility; and simultaneously, the process is simple, and the use is convenient.

The invention belongs to the technical field of biomedical engineering, in particular relates to the technical field of skin wound dressing preparation, and discloses an antibacterial acellular dermal dressing with vascularization inducing capability and a preparation method thereof. The antibacterial acellular dermal matrix dressing comprises porcine acellular dermal matrix, poly dopamine, vascular endothelial growth factor (VEGF) composition and antibacterial peptide LL37. The antibacterial acellular dermal dressing with the vascularization inducing capability has the advantages of low immunogenicity, good biocompatibility and antibacterial ability, and has obvious wound healing and vascularization promoting effect.

The invention discloses a preparation method of a xenogenic acellular dermal matrix. The method specifically includes the following steps of taking dermis, removing epidermal layers, conducting acellular disposal, punching holes through lasers, and conducting sterilizing. By means of the method, cell components are removed thoroughly, antigenicity is low, the dermal collagen structure is kept, histocompatibility is high, the time of the whole preparation process is only about 60 hours, disposal time is short, disposal cost is low, and good social benefits and economic benefits are achieved.

Flowable matrix compositions and methods of their use and manufacture are provided. Exemplary compositions may include a flowable, syringeable, putty-like form of acellular human dermal matrix. In some cases, compositions may include a moldable acellular collagen extracellular matrix. In use, the matrix compositions can be used to fill or treat skin voids, channel wounds, and other soft tissue deficiencies.

The invention relates to the field of gelatin-based biomedical materials, in particular to a preparation method and application of a bionic electronic skin medical stent with a self-repairing function. The method is characterized in that gelatin extracted from an acellular fetal calfskin dermal matrix with good biological safety is used as a raw material; then, conducting polymer poly (3, 4-ethylenedioxythiophene) with good biocompatibility is used; compounding a polystyrene sulfonate (PEDOT: PSS) solution with self-made acidified carbon nanotubes (H-MWCNTs); the hydrogel stent with a multifunctional three-dimensional network space structure is obtained through crosslinking of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and the hydrogel is good in biocompatibility and high in mechanical strength, has both self-repairing performance and tissue regeneration inducing repairing performance and can be widely applied to skin wound repairing treatment.

The invention discloses a breast patch and a preparation method thereof. The preparation method comprises the following steps of: paring and sorting; degreasing and decellularizing; cutting; irradiating and sterilizing. By adopting the preparation method of the breast patch provided by the invention, application of strong acid, strong alkali and enzyme can be avoided, the natural three-dimensional collagen scaffold fiber structure of porcine acellular dermal matrix can be maintained, and the breast patch has excellent mechanical performance to achieve the effect of strengthening or repairing defective tissues. The prepared breast patch has tidy edge and smooth curve, can meet basic requirement for medical appliance products, and guarantees biological safety.

The invention relates to a preparation method of a collagen membrane and the collagen membrane thereof, which belongs to the biology field. The collagen membrane has the compact performance and appropriate degradability, and can effectively protect an acellular dermal matrix layer and avoid wound infection. The membrane comparison overcomes the defects of rarefaction and improper usage as a protection layer for the corium layer of tissue engineering skin in prior art. The preparation method of the collagen membrane comprises the following steps: preparing a collagen solution, wherein pH value of the collagen solution is 68-; gelling the collagen solution to obtain collagen block; concussing and crosslinking the collagen block under effect of a cross-linking agent, and then cleaning; and pressing the cleaned collagen block to obtain the collagen membrane.

Acne-affected skin has been found to be accompanied by the presence of matrix-degrading enzymes such as MMPs and neutrophil elastase, induction of neutrophils, and a reduction in procollagen biosynthesis. This invention treats scarring and inflammation accompanying acne by administering, topically or systemically, at least one of (i) an inhibitor of the matrix degrading enzymes and (ii) a cytokine inhibitor that alleviates inflammation and thus also alleviate neutrophil infiltration. Alleviating the matrix degradation and renormalizing procollagen biosynthesis allows for reduced inflammation and better natural repair of acne-affected skin. Inhibiting cytokines alleviates induction of MMPs in resident skin cells, and also alleviates inflammation with its concommitant induction of neutrophils from the blood stream bringing MMPs and elastase into the acne lesion. Dimishing the presence of matrix-degrading enzymes in the acne lesion reduces imperfect repair of the skin and thus decreases scarring in acne-affected skin.

The invention discloses a xenogenic acellular dermal matrix particle soft tissue filling agent and a preparation method thereof. The product consists of xenogenic acellular dermal matrix particles which are not treated by a cross-linking treatment, xenogenic acellular dermal matrix particles treated by a cross-linking treatment and medical collagen powder. The filling agent disclosed by the invention is convenient to prepare and excellent in filling agent, and is specifically suitable for filling soft tissue for aesthetic and plastic surgery, such as winkle eliminating and depression filling.

UVB radiation of about 300-310 nm wavelength and UVA radiation of about 380-390 nm wavelength, each of which exists in solar light, induces MMPs (matrix metalloproteinases) in human skin that degrade the collagen of the dermal matrix. This degradation contributes to photoaging of human skin, which can be prevented by blocking these wavelengths of solar radiation. In contrast, diseases that result in the overproduction of collagen can be treated by exposing the affected with to radiation having wavelengths in those regions, for these wavelengths not only induce MMPs but also inhibit collagen biosynthesis. For lighter skinned people so affected, the UVA wavelengths are preferred because of the reduced amount of erythema, whereas dark skinned people can be treated with the UVB radiation because they generally do not suffer from erythema.

Soft tissue repair grafts are provided for supporting, covering, and / or retaining an implant positioned in the body of a subject. The grafts are particularly suitable for use for pre-pectoral breast reconstruction with a breast implant or tissue expander. The grafts include positional notches for more accurate positioning in a subject. The grafts also include at least one cuff element which is folded to form a reinforced folded edge for suturing the graft more securely to adjacent tissues than previously known grafts. The grafts also include a plurality of arcuate slots which form a plurality of circular patterns arranged concentrically about a focal point, thereby enabling the grafts to expand without tearing and to conform more closely to the implant and / or adjacent body tissues such as the breast pocket, than previously known grafts. Acellular dermal matrices are particularly suitable for making the soft tissue repair grafts.