31 results about "Dermal matrix" patented technology

There is disclosed a packaged allograft implant configured for implantation in a human recipient. In an embodiment, the packaged allograft implant includes an acellular dermal matrix sheet having a top surface and a bottom surface in opposition to one another, a perimeter surrounding the top surface and the bottom surface, and a thickness extending between the top surface and the bottom surface. The packaged allograft implant further includes a mesh pattern extending across at least a portion of the top surface and the bottom surface of the acellular dermal matrix sheet, the mesh pattern providing through-holes extending between the top surface and the bottom surface of the acellular dermal matrix sheet, and the mesh pattern having a plurality of mesh lines extending in a first direction and a second direction. The first direction and the second direction are orthogonal to one another so as to allow a given amount of stretch in each of the first direction and the second direction. Other embodiments are also disclosed.

Allografts for soft tissue repair, including breast reconstruction and other plastic surgery procedures, are disclosed. In an exemplary embodiment, first and second cuts are made into the reticular layer of a skin sample to separate an improved acellular dermal matrix (ACDM) which forms a dermal tissue form useful as a graft. The dermal tissue form consists essentially of reticular dermis and has a first exposed surface formed by reticular dermis, an opposite second exposed surface formed by reticular dermis, and has substantially uniform density and uniform porosity between the first and second exposed surfaces, which allows and promotes rapid and efficient cellular ingrowth and tissue ingrowth substantially equally from either the first or second exposed surfaces upon implantation.

The invention provides a decellularized dermal matrix particle and a preparation method thereof, the preparation method comprises the steps of pretreatment, degreasing, virus inactivation, decellularization, grinding, crosslinking, freeze-drying and sterilization subpackaging, and solves the problems that the existing decellularized particle product is non-uniform in particle size, incomplete in decellularization, poor in injection effect and incapable of meeting the requirements of tissue repair and regeneration. The invention develops a product which is short in production cycle, relatively concentrated in particle size distribution, thorough in cell removal and complete in matrix function preservation, can effectively remove fat and viruses in animal skin, and has the advantages of being thorough in decellularization, short in time, high in efficiency, light in damage to dermal matrix, capable of removing antigens, capable of retaining a natural structure and capable of effectively guiding tissue regeneration. When the temperature of the freeze-dried particle dispersion liquid is reduced, the water binding capacity of the particles is reduced, free water in the prepared solution is increased, the particles naturally float and gather (dense at the upper part and sparse at the lower part), and the free water is in the lower layer and still has good dispersity after being sterilized.

The invention provides double-crosslinking acellular matrix hydrogel as well as a preparation method and application thereof, and belongs to the technical field of hydrogel. The preparation method comprises the following steps: (1) carrying out decellularization treatment on a porcine dermal layer to obtain a decellularized porcine dermal matrix, (2) putting freeze-dried powder of the decellularized porcine dermal matrix into an acidic pepsin solution, stirring and digesting, adjusting the concentration, adding 10xPBS, adjusting the pH value to obtain a pregel solution, and carrying out freeze-drying on the pregel solution to obtain the porcine dermal matrix freeze-dried powder. (3) putting gel formed by the pre-gel solution into a tannic acid solution for cross-linking to obtain mono-cross-linked acellular matrix hydrogel; (4) smashing the mono-cross-linked acellular matrix hydrogel, and mixing the smashed mono-cross-linked acellular matrix hydrogel with a glucose oxidase solution and a ferrous ion solution with the concentration of 0.1-10mg / ml to obtain a bi-cross-linked acellular matrix hydrogel precursor; 5, the double-crosslinking acellular matrix hydrogel precursor is placed under the condition of glucose with the concentration being 10-200 mg / dl to form the double-crosslinking acellular matrix hydrogel. The hydrogel prepared through the method can achieve the balance of safety, supporting performance and degradability, and is wide in application range.

A composition includes a drying method-applied fibrous acellular dermal matrix. Such a composition includes 48 to 95.5% by weight of a fibrous acellular dermal matrix; and 0.5 to 52% by weight of water. The composition has no biocompatible polymer added thereto. A method for preparing the composition includes steps of: (a) pulverizing an acellular dermal matrix to prepare a fibrous acellular dermal matrix; (b) adding water to the fibrous acellular dermal matrix to rehydrate it; and (c) heat-drying the rehydrated fibrous acellular dermal matrix.

The present invention relates to an apparatus for adjusting the thickness of an acellular dermal matrix, the apparatus being capable of fine adjustment and planarization, and comprising: a main body part; a first support part located in front of the main body part; a second support part which is located in front of the main body part and is adjacent to and located in front of the first support part; a pair of roller parts which each extend in the width direction and which rotate in opposite directions in conjunction with the first support part and the second support part; and a cutter part located between the pair of roller parts. The acellular dermal matrix is inserted between the pair of roller parts, descends vertically due to the rotation of the pair of roller parts, and at the same time, comes into contact with the cutter part and is cut.

The present invention relates to a composition comprising a fibrotic acellular dermal matrix, which does not apply a biocompatible polymer, thereby having improved physical properties and tensile strength, and which is easier to use than in the prior art, and a method for preparing the same.

The present disclosure relates to novel pharmaceutical compositions and their use in general tissue reconstructive and augmentative methods. The pharmaceutical compositions comprise multilayered acellular matrices, such as dermal matrices (ADMs), of two or more layers. The compositions may be used in any general tissue reconstructive or augmentative medical procedure where the goal is to increase the volume in the area of the body in which the multilayered ADM compositions are introduced.

A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes and method of manufacture. A universal biological prosthesis of acellular dermal matrix (ADM) intended for use in body reconstruction processes, comprising: a first layer of ADM of biological origin arranged inferiorly to delimit a cavity with a lower edge; a filling material disposed in the cavity surrounded by the first layer of ADM consisting of a ground ADM having the consistency of a fibrous paste; and a second layer of ADM of biological origin arranged on the upper part covering the filling material and whose upper edge rests peripherally on the lower edge of the first layer of ADM, thus defining a rim;where the first layer of ADM and the second layer of ADM are joined by an eyebrow overlap along the upper edge of the first layer of ADM with the lower edge of the peripheral rim using a biocompatible adhesive, or by absorbable suture; where the first layer of ADM, the second layer of ADM and the ADM filling material are not immunogenic; and where the ADM comprises collagen fibers, fibronectin, elastin, laminin, glycosaminoglycans and hyaluronic acid. Method for its manufacture.

This invention discloses a method for culturing skin organoids for large-scale production and the skin organoids used for large-scale production, belonging to the fields of tissue engineering and biomanufacturing technology. The method involves laying a dermal matrix layer composed of orthogonal double-network hydrogels embedding fibroblasts in a bioreactor containing multiple independently operable culture modules. After inoculating keratinocytes, negative pressure is used to drive a uniform descent of the culture medium level to achieve gas-liquid interface culture. The first network of the orthogonal double-network hydrogel is formed by photocrosslinking of methacrylamide recombinant collagen, and the second network is formed by Michael addition of thiolated polysaccharides and multi-arm polyethylene glycol maleimide. During the culture process, the barrier maturity is assessed in real time by online monitoring of transepithelial resistance, and the negative pressure is controlled accordingly. A single batch can produce 3000 cm³ of skin organoids. 2 The above-mentioned qualified skin organoids have a transepithelial resistance coefficient of variation of less than 10%.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a filler comprising the same, and uses thereof. More specifically, the present invention relates to a filler comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal filler according to the present invention has excellent biological stability and biocompatibility, and extracellular matrix components such as collagen and elastin are significantly preserved therein. In addition, the filling agent also shows excellent biochemical stability, the pH value or the collagen content and the elastin content are kept unchanged in the storage process, and the filling agent also shows physical stability and can continuously maintain injectability. Therefore, the acellular dermal filler can be effectively used as an injectable filler for repair or volume expansion of soft tissue and reduction of wrinkles in the field of plastic and regenerative medicine.

The utility model discloses a water bath device for dermal matrix processing, and belongs to the field of water bath devices. The water bath device for leather matrix processing comprises a discharging assembly, a cleaning assembly is arranged on the discharging assembly, the discharging assembly comprises a discharging box used for storing water and containing leather, a plurality of sets of partition plates are fixedly installed on a roller, and the multiple sets of partition plates form cavities on the outer side of the roller. According to the water bath device for leather matrix processing, water can be stored in the discharging box, so that leather can be wetted and cleaned, meanwhile, the driving assembly and the cleaning assembly are used in cooperation, the roller can rotate, and therefore the leather can be cleaned, and the leather can be cleaned. According to the cleaning device for the leather, the cleaning efficiency of the leather can be further improved, and after the leather is cleaned, water on the leather can be thrown out through the cleaning assembly, so that the effect of removing dampness is achieved.

There is disclosed an acellular dermal matrix (ADM) graft stored as a packaged ADM graft pocket product prepared by a process that includes providing a portion of ADM tissue having a thickness between 1 mm and 2 mm. The process includes scoring the portion of the ADM tissue into a pre-defined shape to form the domed shape ADM graft. The process includes verifying the thickness of the domed shape ADM graft; shaping the domed shape ADM graft to form an ADM graft pocket configured to receive a breast implant. The process includes packaging the ADM graft pocket to form a packaged ADM graft pocket. The process includes irradiating the packaged ADM graft pocket to a sterility assurance level of a desired level to form the packaged ADM graft pocket product. Other embodiments are also disclosed.

The invention relates to the technical field of skin care products, and particularly discloses an anti-aging polypeptide composition as well as a preparation method and application thereof. According to the anti-aging polypeptide composition disclosed by the invention, through a multi-target-point and multi-layer synergistic effect, efficient wrinkle smoothing, tightening and anti-aging are realized. The anti-aging polypeptide composition can act on a neuromuscular joint and a dermal matrix at the same time, and has double effects of immediate improvement and long-term repair. According to the anti-aging polypeptide composition, the active ingredients and the penetration enhancer are synergistic, and compared with exogenous supplement collagen, the anti-aging polypeptide composition can be effectively absorbed in a transdermal mode, enhance the regeneration capacity of autologous collagen, better regulate extracellular matrixes and improve the anti-aging effect of the composition, and the anti-aging polypeptide composition has the advantages that by endogenous stimulation of regeneration of structural protein such as skin collagen, the anti-aging effect of the anti-aging polypeptide composition is improved, and the anti-aging effect of the anti-aging polypeptide composition is improved. The problem of skin laxity and aging caused by loss of structural proteins such as skin collagen is thoroughly solved, and the efficient wrinkle smoothing and anti-aging effects are achieved.

The invention provides a moldable and self-adhesive cartilage repair material and a preparation method thereof, and the preparation method comprises the following steps: (1) uniformly mixing decellularized cartilage particles obtained by treating fresh articular cartilage tissue, a decellularized fibrous dermal matrix obtained by treating fresh skin tissue, mussel protein and purified water to obtain composite slurry; the composite slurry is prepared from the following components in percentage by mass: 1 to 30 weight percent of decellularized cartilage particles, 55 to 73 weight percent of decellularized fibrous dermal matrix, 2 to 5 weight percent of mussel protein and 5 to 21 weight percent of purified water; and (2) carrying out freeze-drying treatment on the composite slurry to obtain the moldable and self-adhesive cartilage repair material. The cartilage repair material provided by the invention retains main components of cartilage, has plasticity and self-adhesion performance, and can effectively simulate a cartilage regeneration repair microenvironment.

The present invention relates to: a composition comprising a fibrous acellular dermal matrix, which does not employ a biocompatible polymer and is subjected to a drying process using a sieve, thereby exhibiting improved physical properties, tensile strength, and suture strength, and thus providing enhanced usability compared to conventional compositions; and a method for preparing same.

The application discloses a preparation method of a collagen matrix multifunctional self-power generation heart valve repair material, and specifically comprises the following steps: firstly, stirring and mixing an ammonium sulfate solution, gelatin, acrylic acid and acrylamide, then stirring and adding N, N-methylene bisacrylamide, and then adopting electrostatic spinning to form a film to obtain a power generation layer; secondly, preparing a bonding layer by using sodium carboxymethyl cellulose and a pyrrole monomer; and finally, using a decellularized pig dermal matrix as a support, sequentially bonding the power generation layer and the bonding layer with a chitosan solution, and the heart valve repair material is prepared. The heart valve repair material prepared by the application has the function of self-power generation, can self-generate a micro-current to implement electric stimulation on a heart valve wound site, further promotes wound healing, and can also reduce the risk of infection and pain of a patient.

To provide a composition comprising a fibrous acellular dermal matrix having enhanced physical properties, which is easily applicable even to a bent wound site, and a method for producing the same.SOLUTION: A composition comprising 48 to 95.5 wt.% of a fibrous acellular dermal matrix and 0.5 to 52 wt.% of water, wherein a biocompatible polymer is not added, an average tensile strength value of 0.2 N / mm2 or more is satisfied, and the composition is a sheet type.SELECTED DRAWING: Figure 1

The present disclosure relates to a method of obtaining a decellularized dermal matrix from a skin graft of a rabbit, hare or beaver, the method comprising the steps of: degreasing the skin graft using a hydrocarbon solvent, perchloroethylene, a modified alcohol or supercritical carbon dioxide, or a combination thereof; liming the degreased graft by immersing the graft in a solution comprising calcium hydroxide, sodium hydroxide, hydrogen peroxide, or a mixture thereof; fleshing the skin graft; optionally, de-ashing the skin graft; incubating the skin graft in an ethylenediamine tetraacetic acid solution; the skin graft is treated with the detergent solution for 30 minutes to 3 hours to obtain a decellularized dermal matrix. The present disclosure also relates to a decellularized dermal matrix obtained by said method; a composition comprising the decellularized dermal matrix for use in a medicament or veterinary medicament; and a dermal-epidermal skin substitute scaffold comprising the disclosed decellularized dermal matrix.

The invention discloses a skin care preparation based on miRNA combination and a delivery system thereof, and belongs to the technical field of skin care. The invention relates to a skin care preparation based on miRNA combination and a delivery system thereof, the skin care preparation comprises at least eight kinds of chemically modified miRNA mimics, the miRNA mimics are highly expressed in young functional stem cell exosomes, the miRNA can regulate and control senescence related genes in a targeted mode, and the miRNA exists in multiple kinds of adult stem cell exosomes but can be screened after being peculiar to abnormal somatic cells; the miRNA composition disclosed by the invention can be used for accurately inhibiting expression of aging marker genes such as p16INK4a and p21Cip1, reducing accumulation of aging cells, blocking release of inflammatory factors such as IL-6 and IL-1beta, and remarkably improving a chronic inflammation microenvironment. On the basis, skin structure repair and regeneration are promoted by activating dermal matrix synthesis pathways (such as collagen and elastin).

The invention provides a striae gravidarum hexagonal matrix therapy method. Firstly, laser energy penetrates through dermis, melanin is smashed, pigment molecules are metabolized, and purplish red lines are eliminated to improve the skin color; secondly, targeted anti-aging is carried out aiming at grain fibroblasts, the dermal matrix capacity is increased, skin circulation is remodeled, subcutaneous cracks are released, the fibroblast activity is stimulated, and collagen is induced to be generated so as to flatten the skin; thirdly, radio frequency energy is used for activating the deep layer of dermis and the epidermal layer, synthesis of collagen, elastin and hyaluronic acid is promoted, regeneration of skin fiber tissue is stimulated, and the fracture surface is repaired for deep renewal; fourthly, damaged skin soft tissues are repaired, red metabolism is accelerated, and epidermis repair is achieved; 5, aiming at postpartum abdominal fat accumulation, matching with proper tightening and shaping equipment to act on subcutaneous fat cells to enable the subcutaneous fat cells to be split, apoptotic and metabolized so as to reduce fat and shape; and finally, the dermal collagenous fibers are shrunk through deep three-dimensional heating, so that the tightening and lifting of abdomen tightening, pulling and anti-aging are realized.

The invention relates to a preparation method of an acellular dermal matrix as well as a product and application of the acellular dermal matrix, the preparation method comprises the following steps: mixing skin tissues with an alkali solution, and removing hair, an epidermal layer and subcutaneous tissues to obtain a dermal layer; and mixing the corium layer with an alkali solution, and cleaning to obtain the corium layer. The method is easy and convenient to operate, the steps are greatly simplified, manpower and cost input is remarkably reduced, damage or residue of enzymes, surfactants and the like to the dermis structure is avoided, main components and the complete structure of the dermis layer are reserved to the maximum extent, and it is ensured that the obtained dermis matrix has good mechanical strength and biological performance.

An acellular dermal matrix patch, a preparation method for the acellular dermal matrix patch, and an application of the acellular dermal matrix patch in repairing tissue damage. The acellular dermal matrix patch comprises a plurality of patch regions, and the patch regions have at least two crosslinking characteristics. The present invention further provides a preparation method for the acellular dermal matrix patch, and an application of the acellular dermal matrix patch in repairing tissue damage.

To provide a composition comprising a fibrous acellular dermal matrix having enhanced physical properties, which is easily applicable even to a bent wound site, and a method for producing the same.SOLUTION: A composition comprising 48 to 95.5 wt.% of a fibrous acellular dermal matrix and 0.5 to 52 wt.% of water, wherein a biocompatible polymer is not added, an average tensile strength value of 0.2 N / mm2 or more is satisfied, and the composition is a sheet type.SELECTED DRAWING: Figure 1

There is disclosed a packaged allograft implant configured for implantation in a human recipient. In an embodiment, the packaged allograft implant includes an acellular dermal matrix sheet having a top surface and a bottom surface in opposition to one another, a perimeter surrounding the top surface and the bottom surface, and a thickness extending between the top surface and the bottom surface. The packaged allograft implant further includes a mesh pattern extending across at least a portion of the top surface and the bottom surface of the acellular dermal matrix sheet, the mesh pattern providing through-holes extending between the top surface and the bottom surface of the acellular dermal matrix sheet, and the mesh pattern having a plurality of mesh lines extending in a first direction and a second direction. The first direction and the second direction are orthogonal to one another so as to allow a given amount of stretch in each of the first direction and the second direction. Other embodiments are also disclosed.

The present invention relates to a dispersion comprising acellular dermal matrix microparticles, a membrane comprising the same, and uses thereof. More specifically, the present invention relates to a membrane comprising a dispersion containing acellular dermal matrix microparticles obtained by supercritical fluid extraction and high pressure dispersion operations, and a method for preparing the same. The acellular dermal membrane according to the present invention exhibits excellent biological stability and biocompatibility, and shows significantly better preservation of extracellular matrix components such as collagen. In addition, the material has excellent mechanical properties, so that the material is easily applied to transplantation operation and suture, and has a porous structure, so that body fluid flow is facilitated, and tissue regeneration can be supported. Thus, the acellular dermal membrane can be applied as wound dressing, adhesion barrier and implantable biomaterials such as drug delivery grafts and artificial skin in the field of regenerative medicine.

The present invention relates to a biomatrix and a manufacturing method therefor, the biomatrix comprising, as active ingredients, a fibrous acellular dermal matrix and a biocompatible material and having a layered cross-section and thus having enhanced physical properties.

The invention discloses a guided tissue regeneration cartilage repair material and a preparation method thereof, and belongs to the technical field of medical materials. The tissue regeneration guided cartilage repair material is composed of three layers of different materials, namely a cartilage repair layer, a semi-permeable isolation layer and a subchondral bone repair layer from top to bottom in sequence, and the three layers of materials are bonded together through a biological adhesive to form a whole. Wherein the cartilage repair layer is composed of homogeneous or heterogeneous cartilage extracellular matrixes, is derived from natural cartilage tissues, is rich in collagen and glycosaminoglycan, has good biocompatibility, multiple growth factors and a vertical pore structure, and is beneficial to adhesion and growth of cells; the semi-permeable isolation layer is composed of a homogeneous or heterogeneous acellular dermal matrix, serves as a barrier, can prevent cells or blood vessels in subchondral bone tissue from growing into cartilage and affecting cartilage repair after being implanted, has permeability, and is beneficial for nutritional ingredients in tissue fluid below the cartilage to permeate into a cartilage area and nourish cartilage cells. The subchondral bone repair layer is a homogeneous or heterogeneous cancellous bone or a decalcified cancellous bone, so that bone tissue repair is facilitated, and clinical operation and fixation are facilitated. According to the three-layer cartilage repair material, in-vivo bone and cartilage repair environments are simulated respectively, bone and cartilage tissues are guided to be synchronously repaired, and the repair effect of articular cartilage defects is improved.