515 results about "Collagen fibres" patented technology

The disclosure describes medical constructs with a plurality of collagen fibers twisted together and methods of twisting the collagen fibers to form the medical constructs.

An aspect of the invention is a split leather product provided with a base material comprising split leather, and a skin layer laminated on a surface of the base material. The skin layer comprises a composite body of an entangled nonwoven fabric formed from microfine fibers, and a polymeric elastomer that impregnates the gaps in the entangled nonwoven fabric. The entangled nonwoven fabric in such a split leather product has the effect of increasing physical strength without detracting from a leather-like texture, in the same way as the longitudinally and transversally crisscrossing collagen fibers in the reticular layer. It is thus possible to obtain a split leather product that resembles leather not only in outward appearance but also in the feel derived from wrinkles and the like resulting when the leather is bent.

At present, researchers do the research of reasonable utilization of chrome-containing leather shavings all the time; however, the leather shavings of leather making enterprises are difficult to treat in batch. The invention provides an environment-friendly collagen fiber leather substrate which is characterized by comprising an intermediate-layer fiber web, a surface-layer fiber web tightly connected on the upper surface of the intermediate-layer fiber web, and a bottom-layer fiber web tightly connected on the lower surface of the intermediate-layer fiber web, wherein the surface-layer fiber web and the bottom-layer fiber web respectively comprise the components by mass percentage of: 90 to 97 percent of collagen fibers, 3 to 10 percent of polylactic acid bi-component fibers; the intermediate-layer fiber web is made of polylactic acid bi-component fibers; the weight of the surface-layer fiber web and the weight of the bottom-layer fiber web are both 30 to 150 grams per square meter; and the weight of the intermediate-layer fiber web is 10 to 30 grams per square meter. The non-woven synthetic leather substrate prepared by using collagen fiber extracted from leather leftovers has good simulation performance, avoids secondary pollution to the environment, and is beneficial to the follow-up coating of PU (polyurethane).

An adsorbing collagen fibre-solidified tannin material for adsorbing and separating metallic ions (Pb, Cd, Hg, Cr and Cu) is prepared from animal skin through preparing collagen fibre membrane or granules, adding the said collagen fibres along with the aqueous solution of tannin to a reactor, reacting at 10-45 deg.c for 6-24 hr, laying aside for 12-24 hr, filtering, washing to remove excessive tannin, adding cross-linking agent, reacting at 20-70 deg.c for 2-8 hr, filtering, washing and drying at 60 deg.c for 12-24 hr. It has very high adsorption selectivity.

Described herein are a device and a method for the treatment of varicose veins via laser radiation, and in particular using a holmium laser. The radiation of a laser source (5) is injected in a fiber (3) that can be inserted in the vessel to be treated. The laser source emits a radiation such as to cause a hyalinizing sclerosis with structural modifications both to the fibers of the collagen (shrinkage) and to the extracellular matrix of the median coat of the vein by the photothermal effect, substantially without thermal stress of the morphological component of the tunica media and of the tunica intima.

The invention discloses a ply tissue engineering corneal frame and a manufacturing method and an application thereof, relating to a ply tissue engineering corneal frame of the biomedicine. Compared with the existing materials, the invention provides a ply tissue engineering corneal frame and a manufacturing method and an application thereof, which has abundant resources, high transparency, good biocompatibility, thorough decellularization and strong safety, and the performance approaches to that of a fresh cornea, so that the ply tissue engineering corneal frame can be accepted by majority of patients and applied clinically for a long term. The ply tissue engineering corneal frame is an animal derived decellularized ply corneal stroma sheet and does not contain cellular constituents; collagenous fibers are tidily arranged, and gaps are regular; corneal light transmittance is 80-95%, and tensile strength is 2-5N/mm<2>. The ply tissue engineering corneal frame can be served as the substitute for various donor materials for corneal transplantation and can be used for treating a series of diseases of corneal trauma and chemical burn, corneal tumor and a series of hyperplastic diseases, a series of diseases of corneal neovascularisation and scar, corneal immune diseases, a series of diseases caused by corneal transplantation exclusive reaction and other keratopathy.

Biomaterials and methods and uses for repair or augmentation of tissues are provided. In particular, the invention provides a multi-layered, naturally occurring multi-axial oriented biomaterial comprising predominately type I collagen fibers. The invention further provides methods and uses for repair or augmentation of tissues using biomaterials of the invention.

The present invention provides a preparation method of high-strength bionic collagen membrane. The preparation method is by conducting gradient dialysis process on collagen to rearrange collagen molecules into an ordered structure, and endow the molecules with more consistent and more extensive collagen fiber orientation, thus improving the mechanical properties of the material. The collagen membrane prepared by using the method has similar appearance, structure and performance to natural dura and amnion of mammals, and good rehydration, and is free to bend and fold after water absorption; the bionic collagen membrane with high tensile strength can be stitched by using suture and can be used in clinical guided tissue regeneration or repair of various tissues.

The invention provides a biological tissue matrix material, and a preparation method and a purpose thereof. The biological tissue matrix material is characterized by comprising an extracellular matrix, wherein the extracellular matrix comprises collagenous fiber, growth factors and fibronectin. The biological tissue matrix material is prepared from small intestinal submucosa matrix materials. The preparation method is characterized by comprising the following steps that decellularization is performed: decellularized liquid contains trypsin and PBS solution, and also contains EDTA, EDTA-2Na or EDTA-4Na; the decellularized liquid is used for treatment in multi-frequency ultrasonic environment for decellularization; the dual-frequency ultrasound at least contains two ultrasonic frequencies with different frequencies. The decellularization process technology is improved; the DNA residual quantity of the obtained product is lower; the immunogenicity is lower; the anti-infection capability is higher; the restoration capability is higher.

The invention relates to a kind of decellularized blood vessel matrix gel, a preparation method therefor and applications thereof. The preparation method is as follows: first, after blood vessel outer membranes and anadesma are removed, the blood vessels are cut into blood vessel pieces, the blood vessel pieces are subjected to decellularized treatment in a TritonX-100 solution, then subjected to freeze drying, smashed into powder, and filtered with a 60-mesh screen, and decellularized blood vessel matrix powder is obtained; second, the decellularized blood vessel matrix powder is placed in a hydrochloric acid solution containing pepsin with a concentration of 0.09-0.11%, and subjected to digestion for 66-78h, a sodium hydroxide solution is added to neutralize hydrochloric acid, then PBS is added to balance ion concentration, finally, the gel solution is placed in an incubator with a temperature of 37 DEG C for gel forming. The decellularized blood vessel matrix gel has good molding capability and mechanical properties, the interior is loose, the gel has certain poriness, and the collagen fiber has appropriate diameters and good stability. The gel can meet requirements of preparation of medicines treating ischemic diseases, support materials of tissue engineering and/or cell culture.

At present, the synthetic leathers are made of artificial fibers, and due to the performance difference between the artificial fibers and real leather collagen fibers, the performances of the artificial leathers are greatly different from the performances of the real leathers. The existing reclaimed leathers have harder handfeel and the environment is polluted in the production processes of the reclaimed leathers. The invention provides a method for producing an artificial leather base material by compounding collagen fibers by utilizing spun-laced water jetting. The method comprises the following steps: 1) firstly preparing the collagen fibers into a collagen fiber sheet for compounding by utilizing spun-laced water jetting; and 2) opening and entangling the collagen fiber sheet by utilizing spun-laced water jetting in a spun-laced nonwoven process, at the same time, compounding the collagen fiber sheet with an opened and carded synthetic fiber sheet, and after compounding, implanting the synthetic fibers in the collagen fibers so that the two different fiber sheets are firmly bonded as a whole. The method has the following beneficial effects: after undergoing the subsequent coating processing, the obtained base material has stronger leather feel and better simulation than the traditional synthetic leathers and softer handfeel than the reclaimed leathers; and the method is more environment-friendly than the traditional production methods of the reclaimed leathers.

The invention relates to a casing of meat sausage product, and especially discloses a production method of collagen casing. The production method comprises following steps of washing the leather materials to be used, softening the leather materials by liming, water washing, cutting, removing calcium salt, puffing, extracting collagen, stirring, extruding and moulding, pre-solidifying, water washing, drying and fixing, folding and packaging. The invention is aimed to provide a collagen casing, which is produced by a special technology. The technology takes animal skins such as pigs or cows as the raw materials and soybean protein as the auxiliary material. The collagen casing has the advantages of natural flavor and color, unique taste and safe edibility. The preparation method overcomes the shortage of overlarge contraction by adopting part skins with relative large protein denaturation and adding a certain amount of short fiber protein such as soybean protein; through adding a great amount of water, the collagen solid is less, so that viscosity is low, treatment is convenient, and errors of product indexes are correspondingly reduced.

The invention discloses antigen-free porcine dermal collagen fibers and a preparation method thereof. The antigen-free porcine dermal collagen fibers with excellent performance and wide application are prepared by using purified porcine skin as a raw material and adopting a method of combining physics, chemistry and biochemistry to perform a series of treatment such as fluffing, fiber decomposition, fiber separation, carding and the like on the purified porcine skin on the premise of ensuring good mechanical property of the purified porcine skin unchangeable. The antigen-free porcine dermal collagen fibers have good biocompatibility, good mechanical property and proper degradability, and can be widely applied to preparation of medicinal biological materials such as tissue engineering scaffold materials, hemostatic materials, medicinal operation stitches and the like.

Methods and compositions are described for organizing collagen into fibrillar networks, e.g, short and long-range organization. Collagen produced by the disclosed methods can be used for tissue engineering.

The invention provides a biocompatible article having a surface comprising collagen fibrils attached to said surface via one or more linker molecules, wherein each of said collagen fibrils is attached to at least one of said one or more linker molecules at a proximal end of the fibril, and wherein each of said collagen fibril has a proximal portion extending from said proximal end to a point P along said fibril, wherein, for a majority of said fibrils, each fibril at said point P is oriented so as to form an angle α_P in the range of 0° to 45° to the surface normal N at the point of attachment of said fibril to said surface. The collagen fibril-coated surface has improved biocompatibility and is useful in a medical implant intended for implantation into soft tissue or bone tissue.

The present invention relates to a solid composition useful for tissue gluing, tissue sealing and haemostasis consisting essentially of a) a carrier which has at least one of the following physical properties: elasticity module in the range of 5-100 N/cm, density of 1-10 mg/cm3, chamber diameter of more than 0.75 mm and less than 4 mm and/or having a chamber diameter average below 3 mm and evenly distributed and fixed upon said carrier, b) solid fibrinogen, and c) solid thrombin. The carrier is a biodegradable polymer such as a polyhyaluronic acid, polyhydroxy acid, e.g. lactic acid, glucolic acid, hydroxybutanoic acid, a cellulose, gelatine or collagen, such as a collagen sponge, e.g. a collagen sponge consisting essentially of collagen type I fibres. The fibrinogen and thrombin are preferably human, purified from a natural source, or transgenic or recombinant human fibrinogen and/or thrombin. In a preferred embodiment the composition does not comprise any antifibronolytic agent such as aprotinin, epsi-aminocaproic acid or alpha2-antiplasmin,

Relating to the fields of biotechnologies and gene therapy, the invention provides application of a gene modified mesenchymal stem cell in pulmonary fibrosis treatment. The gene modified mesenchymal stem cells are obtained through: in-vitro isolated culture and amplification of a mesenchymal stem cell (MSC) deriving from bone marrow and an umbilical cord, and recombinant adenovirus Ad-HGF mediated in-vitro modification of the MSC by a hepatocyte growth factor (HGF). By transplanting the gene modified MSC to a C57 mouse to intervene in radiation induced lung injury and fibrosis, exudation of a plurality proteins including albumin, IgM and the like from an alveolar space can be reduced, local inflammatory responses of the lung can be alleviated, and expression of TNF-alpha, soluble ICAM-1 and multiple factors is inhibited, expression of the profibrotic factor TGF-beta, the collagen gene col1 alpha 1 and col 3 alpha 1 can be inhibited, and pulmonary tissue collagen fiber deposition is reduced. The expression results of endogenous HGF and its receptor cmet show that endogenous HGF expression can be induced and endogenous MSC can home to injured parts. Therefore, the employment of HGF modified MSC in treatment of lung injury and fibrosis brought by various pathogenic causes is of great significance.